Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013163.t1 | XP_003602787.1 | 96.3 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 2.10E-290 | 1007.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013163.t1 | Q8W475 | 47.3 | 529 | 246 | 9 | 11 | 534 | 8 | 508 | 6.2e-118 | 426.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013163.t1 | G7J3D7 | 96.3 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 1.5e-290 | 1007.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013163.t1 | MTR_3g099000 | 96.296 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 0.0 | 1072 |
| MS.gene013163.t1 | MTR_3g116120 | 28.409 | 528 | 258 | 12 | 9 | 524 | 50 | 469 | 2.50e-56 | 196 |
| MS.gene013163.t1 | MTR_5g085170 | 27.324 | 527 | 245 | 11 | 7 | 524 | 22 | 419 | 1.14e-49 | 177 |
| MS.gene013163.t1 | MTR_3g088480 | 27.072 | 543 | 325 | 15 | 15 | 525 | 170 | 673 | 4.20e-44 | 167 |
| MS.gene013163.t1 | MTR_4g069830 | 28.571 | 287 | 175 | 5 | 16 | 290 | 139 | 407 | 2.36e-30 | 126 |
| MS.gene013163.t1 | MTR_4g069830 | 28.571 | 287 | 175 | 5 | 16 | 290 | 139 | 407 | 2.40e-30 | 126 |
| MS.gene013163.t1 | MTR_6g053260 | 43.860 | 114 | 58 | 1 | 2 | 115 | 17 | 124 | 2.28e-22 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013163.t1 | AT2G47620 | 49.353 | 541 | 234 | 11 | 1 | 534 | 1 | 508 | 2.11e-151 | 444 |
| MS.gene013163.t1 | AT2G47620 | 50.605 | 413 | 169 | 8 | 1 | 410 | 1 | 381 | 3.88e-120 | 360 |
| MS.gene013163.t1 | AT2G33610 | 26.336 | 524 | 281 | 10 | 7 | 527 | 39 | 460 | 5.20e-52 | 184 |
| MS.gene013163.t1 | AT1G21700 | 27.462 | 528 | 328 | 17 | 15 | 527 | 175 | 662 | 4.12e-47 | 176 |
| MS.gene013163.t1 | AT4G34430 | 26.689 | 296 | 178 | 4 | 15 | 295 | 144 | 415 | 2.79e-26 | 114 |
| MS.gene013163.t1 | AT4G34430 | 26.689 | 296 | 178 | 4 | 15 | 295 | 144 | 415 | 2.79e-26 | 114 |
| MS.gene013163.t1 | AT4G34430 | 26.689 | 296 | 178 | 4 | 15 | 295 | 144 | 415 | 2.87e-26 | 114 |
| MS.gene013163.t1 | AT4G34430 | 26.689 | 296 | 178 | 4 | 15 | 295 | 144 | 415 | 2.94e-26 | 114 |
Find 116 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATATTCTTTGTAGATTT+TGG | 0.062137 | 3.3:-81425301 | None:intergenic |
| GAAATAGGCTTCAACGAATT+TGG | 0.175854 | 3.3:-81425573 | None:intergenic |
| TCGAGTTCTGAATCTGAAAT+TGG | 0.191544 | 3.3:-81425092 | None:intergenic |
| AACTCATTGAGCTGCCCTTT+GGG | 0.243008 | 3.3:+81427191 | MS.gene013163:CDS |
| GTTCAATCTCTCTATCTTCT+TGG | 0.261575 | 3.3:-81435500 | None:intergenic |
| CACTATTACTGTTATCATTT+CGG | 0.270449 | 3.3:-81427237 | None:intergenic |
| CCTGTGTCATTGAAGTTTCT+TGG | 0.272832 | 3.3:-81427312 | None:intergenic |
| CTGCCATTGGAACGGCTCTT+GGG | 0.282371 | 3.3:+81435445 | MS.gene013163:CDS |
| GTATCCCGCGGCAACGAAAT+AGG | 0.302183 | 3.3:-81425588 | None:intergenic |
| GATAACAGTAATAGTGTTAC+TGG | 0.321482 | 3.3:+81427244 | MS.gene013163:CDS |
| CCCTTTGGGGAGCTCATGTT+AGG | 0.346283 | 3.3:+81427205 | MS.gene013163:CDS |
| GCTGCCATTGGAACGGCTCT+TGG | 0.353657 | 3.3:+81435444 | MS.gene013163:CDS |
| ATGGTGGTAGTGCTGGGAGC+GGG | 0.366820 | 3.3:+81425622 | MS.gene013163:CDS |
| TGTGGTGATAAATGTGGTTC+TGG | 0.370702 | 3.3:+81425729 | MS.gene013163:CDS |
| AAACTCATTGAGCTGCCCTT+TGG | 0.381346 | 3.3:+81427190 | MS.gene013163:CDS |
| GTTGAGGAAGGTGCTCCTAA+TGG | 0.394494 | 3.3:+81425531 | MS.gene013163:CDS |
| GCATCGTATTCAGATGTTTA+CGG | 0.394898 | 3.3:+81425669 | MS.gene013163:CDS |
| CGCCTTCTGCTGGTGACGGT+GGG | 0.398334 | 3.3:+81425466 | MS.gene013163:CDS |
| GGTAGTGCTGGGAGCGGGGT+TGG | 0.399459 | 3.3:+81425627 | MS.gene013163:CDS |
| TGAATGAAAATCCTTCAAAA+AGG | 0.402501 | 3.3:+81427383 | MS.gene013163:CDS |
| TTAAATCTGGTGTCGCTAGA+TGG | 0.405889 | 3.3:+81435810 | MS.gene013163:CDS |
| GCTGCAGCAGCTGTGATATG+AGG | 0.409519 | 3.3:-81427930 | None:intergenic |
| AGCAACTATAATTGATGCTC+AGG | 0.419562 | 3.3:+81435530 | MS.gene013163:CDS |
| AACAGAGAAAGCCGCATTCA+AGG | 0.419615 | 3.3:+81425245 | MS.gene013163:CDS |
| TGCTGGTGACGGTGGGGAAG+CGG | 0.423290 | 3.3:+81425473 | MS.gene013163:CDS |
| GTGAATGTTAACCTTCTAGA+AGG | 0.426633 | 3.3:-81425354 | None:intergenic |
| CTGCTTCATTAGTGAACTGC+TGG | 0.426983 | 3.3:-81427431 | None:intergenic |
| ATTGCTGATGGTGGTAGTGC+TGG | 0.428983 | 3.3:+81425615 | MS.gene013163:CDS |
| GGAGAAGGAACACGCTGAAA+TGG | 0.441692 | 3.3:+81435733 | MS.gene013163:CDS |
| CGAGTTCTGAATCTGAAATT+GGG | 0.442640 | 3.3:-81425091 | None:intergenic |
| GTGCCAACCACACTGCTGTC+TGG | 0.444849 | 3.3:+81427059 | MS.gene013163:CDS |
| TTGAGGAAGGTGCTCCTAAT+GGG | 0.445261 | 3.3:+81425532 | MS.gene013163:CDS |
| AGAGGAGCGGTGTAAGTTGA+AGG | 0.454875 | 3.3:+81425509 | MS.gene013163:CDS |
| TGATTTGATTCGACGTAAAG+AGG | 0.456139 | 3.3:+81425692 | MS.gene013163:CDS |
| AACTACGGTGCGCCTTCTGC+TGG | 0.458132 | 3.3:+81425456 | MS.gene013163:CDS |
| GCAACAACACGAATCCCATT+AGG | 0.458762 | 3.3:-81425546 | None:intergenic |
| TGACGGTGGGGAAGCGGAGA+AGG | 0.465624 | 3.3:+81425479 | MS.gene013163:CDS |
| GAACAATGGAAACAAAGTTC+AGG | 0.466829 | 3.3:+81427273 | MS.gene013163:CDS |
| AACCCCAACGCTCCAAGAAA+AGG | 0.466969 | 3.3:-81425428 | None:intergenic |
| TCAACATCAAATATATCCCT+TGG | 0.467931 | 3.3:-81427993 | None:intergenic |
| ATCGCGAAGAACCTTCTAGA+AGG | 0.477685 | 3.3:+81425343 | MS.gene013163:CDS |
| GATGGTGGTAGTGCTGGGAG+CGG | 0.482401 | 3.3:+81425621 | MS.gene013163:CDS |
| GTCAGGGCTCTCGAGGCTGA+AGG | 0.483976 | 3.3:+81428163 | MS.gene013163:intron |
| CATTGAAGTTTCTTGGTGAT+CGG | 0.484104 | 3.3:-81427305 | None:intergenic |
| ACGGGAATTATGGGGAGAAA+AGG | 0.485836 | 3.3:+81426996 | MS.gene013163:CDS |
| AATACATTCAAGTTTAGTCT+TGG | 0.490532 | 3.3:-81427164 | None:intergenic |
| TTGCGGAAGACATTTAAATC+TGG | 0.491955 | 3.3:+81435797 | MS.gene013163:CDS |
| CGTTGGGGTTTGATTAACTA+CGG | 0.494430 | 3.3:+81425441 | MS.gene013163:CDS |
| GTGGAGGGGTCCACTCAATC+AGG | 0.498019 | 3.3:+81428196 | MS.gene013163:CDS |
| TGCTGCACGAGCAAAGTTGT+TGG | 0.507153 | 3.3:+81435473 | MS.gene013163:CDS |
| AGTGCTTACTGGAAGAGCTT+GGG | 0.513344 | 3.3:-81425127 | None:intergenic |
| ATTTAATTTGAAATCCTCCA+TGG | 0.517576 | 3.3:-81427020 | None:intergenic |
| GAGTGCTTACTGGAAGAGCT+TGG | 0.522921 | 3.3:-81425128 | None:intergenic |
| CAGCAGTTCACTAATGAAGC+AGG | 0.523188 | 3.3:+81427432 | MS.gene013163:CDS |
| GCGCCTTCTGCTGGTGACGG+TGG | 0.524490 | 3.3:+81425465 | MS.gene013163:CDS |
| TGGTGTTAAAATTGCGCCGT+TGG | 0.524858 | 3.3:+81425647 | MS.gene013163:CDS |
| CGACGTAAAGAGGTCAATTG+TGG | 0.526288 | 3.3:+81425702 | MS.gene013163:CDS |
| GACGTAAAGAGGTCAATTGT+GGG | 0.526317 | 3.3:+81425703 | MS.gene013163:CDS |
| CAACATCAAATATATCCCTT+GGG | 0.527017 | 3.3:-81427992 | None:intergenic |
| TGCCATTGGAACGGCTCTTG+GGG | 0.528099 | 3.3:+81435446 | MS.gene013163:CDS |
| GCTCTTATACGTAGTGTCAA+TGG | 0.528104 | 3.3:-81435423 | None:intergenic |
| CACACTGCTGTCTGGACTGA+GGG | 0.532324 | 3.3:+81427067 | MS.gene013163:CDS |
| GAAGCCTATTTCGTTGCCGC+GGG | 0.539844 | 3.3:+81425584 | MS.gene013163:CDS |
| TGGTGGTAGTGCTGGGAGCG+GGG | 0.541162 | 3.3:+81425623 | MS.gene013163:CDS |
| GTGATGAGAATTCGCTCCCA+AGG | 0.542135 | 3.3:+81427976 | MS.gene013163:CDS |
| TGCACGAGCAAAGTTGTTGG+CGG | 0.551025 | 3.3:+81435476 | MS.gene013163:CDS |
| GGTTGAGAAGTTGCAACAGA+AGG | 0.551352 | 3.3:+81435679 | MS.gene013163:intron |
| TGAAGGTTTAGAGATGGTGG+AGG | 0.556011 | 3.3:+81428180 | MS.gene013163:CDS |
| GGATATATTTGATGTTGAAG+AGG | 0.561648 | 3.3:+81427998 | MS.gene013163:CDS |
| AGCGGAGAAGGAACATGAAG+AGG | 0.562741 | 3.3:+81425491 | MS.gene013163:CDS |
| GCGGTGTAAGTTGAAGGTTG+AGG | 0.565042 | 3.3:+81425515 | MS.gene013163:CDS |
| CTGCAGCAGCTGTGATATGA+GGG | 0.565802 | 3.3:-81427929 | None:intergenic |
| ACTCATTGAGCTGCCCTTTG+GGG | 0.569298 | 3.3:+81427192 | MS.gene013163:CDS |
| GGCTGAAGGTTTAGAGATGG+TGG | 0.570333 | 3.3:+81428177 | MS.gene013163:CDS |
| AGCCCCAAGAGCCGTTCCAA+TGG | 0.577527 | 3.3:-81435448 | None:intergenic |
| CCTAACATGAGCTCCCCAAA+GGG | 0.577653 | 3.3:-81427205 | None:intergenic |
| GCCTTCTGCTGGTGACGGTG+GGG | 0.578188 | 3.3:+81425467 | MS.gene013163:CDS |
| TGACGTCACTTTCTTGAACA+AGG | 0.580657 | 3.3:+81425401 | MS.gene013163:CDS |
| GAAGGTTTAGAGATGGTGGA+GGG | 0.582672 | 3.3:+81428181 | MS.gene013163:CDS |
| TCCCCACCGTCACCAGCAGA+AGG | 0.584174 | 3.3:-81425468 | None:intergenic |
| CTACGTATAAGAGCTGCCAT+TGG | 0.589808 | 3.3:+81435432 | MS.gene013163:CDS |
| AAGATTCTATCTTAACTGAA+CGG | 0.593295 | 3.3:+81435765 | MS.gene013163:CDS |
| TATCTTTGTCAGGGCTCTCG+AGG | 0.594795 | 3.3:+81428156 | MS.gene013163:intron |
| TTGCTGATGGTGGTAGTGCT+GGG | 0.596207 | 3.3:+81425616 | MS.gene013163:CDS |
| AGTCCAGACAGCAGTGTGGT+TGG | 0.597006 | 3.3:-81427062 | None:intergenic |
| GGTGCGCCTTCTGCTGGTGA+CGG | 0.597438 | 3.3:+81425462 | MS.gene013163:CDS |
| TGATGAGAATTCGCTCCCAA+GGG | 0.601664 | 3.3:+81427977 | MS.gene013163:CDS |
| TGAAGCCTATTTCGTTGCCG+CGG | 0.604522 | 3.3:+81425583 | MS.gene013163:CDS |
| CGAGGCTGAAGGTTTAGAGA+TGG | 0.605211 | 3.3:+81428174 | MS.gene013163:CDS |
| GCCTAACATGAGCTCCCCAA+AGG | 0.608377 | 3.3:-81427206 | None:intergenic |
| CCAAGAAACTTCAATGACAC+AGG | 0.608710 | 3.3:+81427312 | MS.gene013163:CDS |
| TGGACATTACCGAAGTAGCA+AGG | 0.608925 | 3.3:+81425749 | MS.gene013163:CDS |
| TTTGAAGCATGGGGATGACT+GGG | 0.610350 | 3.3:+81427117 | MS.gene013163:CDS |
| GGTGGGACTGCTCTCAACTG+TGG | 0.615676 | 3.3:+81427902 | MS.gene013163:intron |
| GAGGTCAATTGTGGGAATTG+TGG | 0.618187 | 3.3:+81425711 | MS.gene013163:CDS |
| TATAAGAGCTGCCATTGGAA+CGG | 0.624681 | 3.3:+81435437 | MS.gene013163:CDS |
| GCATTCAAGGAGTATTTCGA+CGG | 0.625023 | 3.3:+81425258 | MS.gene013163:CDS |
| CGGGATACGAAGATTGCTGA+TGG | 0.626607 | 3.3:+81425603 | MS.gene013163:CDS |
| CCACACTGCTGTCTGGACTG+AGG | 0.629638 | 3.3:+81427066 | MS.gene013163:CDS |
| TTATGGGGAGAAAAGGTCCA+TGG | 0.637503 | 3.3:+81427003 | MS.gene013163:CDS |
| GGGAATTGTGGTGATAAATG+TGG | 0.639547 | 3.3:+81425723 | MS.gene013163:CDS |
| TGTAAGTTGAAGGTTGAGGA+AGG | 0.646841 | 3.3:+81425519 | MS.gene013163:CDS |
| GTTACTGGCATTGTGAACAA+TGG | 0.649639 | 3.3:+81427259 | MS.gene013163:CDS |
| AGAAGGAACATGAAGAGGAG+CGG | 0.666643 | 3.3:+81425496 | MS.gene013163:CDS |
| GATACGAAGATTGCTGATGG+TGG | 0.670531 | 3.3:+81425606 | MS.gene013163:CDS |
| ACATCTGAATACGATGCCAA+CGG | 0.670745 | 3.3:-81425663 | None:intergenic |
| GTCGAAATACTCCTTGAATG+CGG | 0.672398 | 3.3:-81425256 | None:intergenic |
| CTGAACGGATTGATGTGTTG+CGG | 0.676867 | 3.3:+81435780 | MS.gene013163:CDS |
| TCAGCAATCTTCGTATCCCG+CGG | 0.680115 | 3.3:-81425600 | None:intergenic |
| CTTACTGGAAGAGCTTGGGA+TGG | 0.682997 | 3.3:-81425123 | None:intergenic |
| AAGAGGATAATGCTTCAGCA+AGG | 0.686587 | 3.3:+81428015 | MS.gene013163:CDS |
| GAGGTACGAAAATCACTCGT+CGG | 0.686796 | 3.3:+81425378 | MS.gene013163:CDS |
| CCTCAGTCCAGACAGCAGTG+TGG | 0.701720 | 3.3:-81427066 | None:intergenic |
| AAGGTTTAGAGATGGTGGAG+GGG | 0.704288 | 3.3:+81428182 | MS.gene013163:CDS |
| TGGGGAGAAAAGGTCCATGG+AGG | 0.741307 | 3.3:+81427006 | MS.gene013163:CDS |
| TAGAAGGTTAACATTCACCG+AGG | 0.759653 | 3.3:+81425359 | MS.gene013163:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 81425074 | 81435859 | 81425074 | ID=MS.gene013163 |
| chr3.3 | mRNA | 81425074 | 81435859 | 81425074 | ID=MS.gene013163.t1;Parent=MS.gene013163 |
| chr3.3 | exon | 81425074 | 81425140 | 81425074 | ID=MS.gene013163.t1.exon1;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81425074 | 81425140 | 81425074 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| chr3.3 | exon | 81425217 | 81425770 | 81425217 | ID=MS.gene013163.t1.exon2;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81425217 | 81425770 | 81425217 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| chr3.3 | exon | 81426962 | 81427453 | 81426962 | ID=MS.gene013163.t1.exon3;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81426962 | 81427453 | 81426962 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| chr3.3 | exon | 81427903 | 81428036 | 81427903 | ID=MS.gene013163.t1.exon4;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81427903 | 81428036 | 81427903 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| chr3.3 | exon | 81428168 | 81428217 | 81428168 | ID=MS.gene013163.t1.exon5;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81428168 | 81428217 | 81428168 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| chr3.3 | exon | 81435406 | 81435551 | 81435406 | ID=MS.gene013163.t1.exon6;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81435406 | 81435551 | 81435406 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| chr3.3 | exon | 81435680 | 81435859 | 81435680 | ID=MS.gene013163.t1.exon7;Parent=MS.gene013163.t1 |
| chr3.3 | CDS | 81435680 | 81435859 | 81435680 | ID=cds.MS.gene013163.t1;Parent=MS.gene013163.t1 |
| Gene Sequence |
| Protein sequence |
