AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061958


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061958.t1 MTR_6g032820 97.087 103 3 0 1 103 12 114 5.07e-67 207
MS.gene061958.t1 MTR_7g085390 74.757 103 25 1 1 103 12 113 8.01e-47 156
MS.gene061958.t1 MTR_1g010120 55.769 104 44 2 1 103 12 114 2.59e-30 112
MS.gene061958.t1 MTR_4g112025 55.340 103 41 3 2 103 21 119 1.28e-27 105
MS.gene061958.t1 MTR_3g108600 56.989 93 38 2 12 103 28 119 3.58e-27 103
MS.gene061958.t1 MTR_1g053320 58.889 90 35 2 15 103 30 118 4.17e-25 97.8
MS.gene061958.t1 MTR_8g092070 51.613 93 43 2 12 103 31 122 2.66e-24 95.9
MS.gene061958.t1 MTR_3g095050 50.538 93 43 3 12 103 31 121 3.37e-23 92.4
MS.gene061958.t1 MTR_3g031770 53.659 82 37 1 13 93 25 106 2.82e-21 87.0
MS.gene061958.t1 MTR_2g027560 48.913 92 45 2 13 103 26 116 9.06e-21 85.5
MS.gene061958.t1 MTR_4g124440 46.512 86 45 1 14 98 27 112 1.25e-20 85.1
MS.gene061958.t1 MTR_3g080410 41.573 89 45 2 13 97 24 109 3.73e-14 66.6
MS.gene061958.t1 MTR_4g114850 35.443 79 50 1 16 93 27 105 1.33e-13 65.1
MS.gene061958.t1 MTR_1g007810 36.170 94 57 2 1 93 4 95 1.63e-13 65.1
MS.gene061958.t1 MTR_4g114850 35.443 79 50 1 16 93 27 105 1.85e-13 64.7
MS.gene061958.t1 MTR_4g114850 35.443 79 50 1 16 93 27 105 2.24e-13 64.7
MS.gene061958.t1 MTR_1g077990 35.802 81 52 0 13 93 29 109 8.30e-13 63.2
MS.gene061958.t1 MTR_5g085580 40.000 80 47 1 15 93 25 104 1.61e-12 62.4
MS.gene061958.t1 MTR_1g031900 40.260 77 45 1 15 90 24 100 1.99e-12 62.0
MS.gene061958.t1 MTR_4g010200 37.975 79 48 1 16 93 34 112 2.03e-12 62.0
MS.gene061958.t1 MTR_1g056640 38.554 83 44 2 15 93 38 117 2.61e-12 61.6
MS.gene061958.t1 MTR_1g031900 40.260 77 45 1 15 90 24 100 3.79e-12 61.2
MS.gene061958.t1 MTR_4g084480 31.395 86 59 0 8 93 25 110 5.84e-12 60.5
MS.gene061958.t1 MTR_4g084480 31.395 86 59 0 8 93 25 110 6.29e-12 60.5
MS.gene061958.t1 MTR_4g084480 31.395 86 59 0 8 93 18 103 7.36e-12 60.5
MS.gene061958.t1 MTR_7g026340 36.145 83 52 1 12 93 20 102 7.38e-12 60.5
MS.gene061958.t1 MTR_5g078200 36.250 80 50 1 15 93 23 102 1.07e-11 60.1
MS.gene061958.t1 MTR_3g065460 36.250 80 50 1 15 93 24 103 2.31e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061958.t1 AT1G64760 78.022 91 19 1 12 102 22 111 3.06e-41 141
MS.gene061958.t1 AT1G64760 78.022 91 19 1 12 102 22 111 3.06e-41 141
MS.gene061958.t1 AT2G19440 76.923 91 20 1 12 102 18 107 3.16e-40 139
MS.gene061958.t1 AT2G19440 76.923 91 20 1 12 102 18 107 3.16e-40 139
MS.gene061958.t1 AT3G04010 59.434 106 39 2 1 103 16 120 2.72e-35 125
MS.gene061958.t1 AT5G18220 69.880 83 25 0 12 94 27 109 4.41e-35 125
MS.gene061958.t1 AT5G64790 57.692 104 42 2 1 103 14 116 2.99e-31 114
MS.gene061958.t1 AT5G58090 57.778 90 36 2 15 103 22 110 1.33e-27 104
MS.gene061958.t1 AT5G20870 53.684 95 43 1 5 98 20 114 4.51e-27 103
MS.gene061958.t1 AT4G31140 48.039 102 51 2 3 103 15 115 6.01e-26 100
MS.gene061958.t1 AT3G24330 53.333 90 40 2 15 103 39 127 9.60e-23 91.3
MS.gene061958.t1 AT5G58480 51.807 83 39 1 12 93 23 105 3.79e-22 89.7
MS.gene061958.t1 AT4G17180 46.512 86 42 2 14 99 24 105 2.03e-18 79.0
MS.gene061958.t1 AT5G42100 42.683 82 47 0 12 93 24 105 1.17e-15 71.2
MS.gene061958.t1 AT5G42100 42.683 82 47 0 12 93 24 105 1.18e-15 71.2
MS.gene061958.t1 AT5G55180 39.759 83 43 2 15 93 27 106 5.57e-14 66.6
MS.gene061958.t1 AT5G55180 38.462 91 47 3 9 93 19 106 8.65e-14 65.9
MS.gene061958.t1 AT2G01630 38.750 80 48 1 15 93 23 102 8.91e-14 65.9
MS.gene061958.t1 AT2G01630 38.750 80 48 1 15 93 23 102 1.47e-13 65.1
MS.gene061958.t1 AT2G01630 38.750 80 48 1 15 93 23 102 1.74e-13 65.1
MS.gene061958.t1 AT2G05790 41.975 81 46 1 15 94 25 105 2.55e-13 64.7
MS.gene061958.t1 AT3G15800 35.484 93 57 2 11 102 39 129 4.62e-13 63.9
MS.gene061958.t1 AT4G26830 37.500 80 49 1 15 93 25 104 7.41e-13 63.2
MS.gene061958.t1 AT4G26830 37.500 80 49 1 15 93 29 108 9.47e-13 62.8
MS.gene061958.t1 AT2G26600 34.524 84 54 1 11 93 30 113 2.06e-12 62.0
MS.gene061958.t1 AT1G32860 34.177 79 52 0 15 93 29 107 3.19e-12 61.6
MS.gene061958.t1 AT1G11820 35.000 80 51 1 15 93 44 123 1.91e-11 59.3
MS.gene061958.t1 AT1G11820 35.000 80 51 1 15 93 44 123 1.96e-11 59.3
MS.gene061958.t1 AT1G11820 35.000 80 51 1 15 93 44 123 2.48e-11 58.9
MS.gene061958.t1 AT4G18340 29.592 98 64 1 1 93 15 112 3.39e-11 58.5
MS.gene061958.t1 AT4G18340 29.592 98 64 1 1 93 15 112 3.77e-11 58.5
MS.gene061958.t1 AT1G30080 29.126 103 71 1 2 102 23 125 5.27e-11 57.8
MS.gene061958.t1 AT1G30080 29.126 103 71 1 2 102 23 125 5.29e-11 57.8
MS.gene061958.t1 AT3G13560 36.250 80 50 1 15 93 26 105 7.29e-11 57.8
MS.gene061958.t1 AT3G13560 36.250 80 50 1 15 93 26 105 7.29e-11 57.8
MS.gene061958.t1 AT3G13560 36.250 80 50 1 15 93 26 105 7.29e-11 57.8
MS.gene061958.t1 AT3G13560 36.250 80 50 1 15 93 26 105 7.29e-11 57.8
MS.gene061958.t1 AT3G13560 36.250 80 50 1 15 93 26 105 7.29e-11 57.8

Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCAGCTAATTGATTATT+AGG 0.201538 6.2:+20956674 None:intergenic
AACATTTGTACAACTGTATC+TGG 0.259099 6.2:+20956794 None:intergenic
GGAAGGACTTGGTGTGAATT+GGG 0.317702 6.2:-20956843 MS.gene061958:CDS
TGGAAGGACTTGGTGTGAAT+TGG 0.327363 6.2:-20956844 MS.gene061958:CDS
GTACAAATGTTGAAAGATAA+TGG 0.351290 6.2:-20956782 MS.gene061958:CDS
ACTCGTTATAACTTCAAATC+TGG 0.376451 6.2:-20956608 MS.gene061958:CDS
ATGAGTGCACTTGGTGGGTC+AGG 0.429886 6.2:-20956719 MS.gene061958:CDS
TTATGATCGTGCTTTGCAAT+GGG 0.469916 6.2:-20956645 MS.gene061958:CDS
ACAACTGTATCTGGTTTCAA+TGG 0.506632 6.2:+20956803 None:intergenic
ATTATGATCGTGCTTTGCAA+TGG 0.517702 6.2:-20956646 MS.gene061958:CDS
TGGGTCAGGTATTGAAGTAA+TGG 0.555721 6.2:-20956705 MS.gene061958:CDS
CGTTATAACTTCAAATCTGG+TGG 0.565387 6.2:-20956605 MS.gene061958:CDS
AAACTATGAGTGCACTTGGT+GGG 0.583255 6.2:-20956724 MS.gene061958:CDS
GAAGGACTTGGTGTGAATTG+GGG 0.620435 6.2:-20956842 MS.gene061958:CDS
GAAGAAACTATGAGTGCACT+TGG 0.642757 6.2:-20956728 MS.gene061958:CDS
GAAACTATGAGTGCACTTGG+TGG 0.667838 6.2:-20956725 MS.gene061958:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCAGCTAATTGATTATT+AGG + chr6.2:20956796-20956815 None:intergenic 20.0%
! GTACAAATGTTGAAAGATAA+TGG - chr6.2:20956685-20956704 MS.gene061958:CDS 25.0%
!!! TGATGATGAGTACAATTTTT+TGG - chr6.2:20956596-20956615 MS.gene061958:CDS 25.0%
AACATTTGTACAACTGTATC+TGG + chr6.2:20956676-20956695 None:intergenic 30.0%
ACTCGTTATAACTTCAAATC+TGG - chr6.2:20956859-20956878 MS.gene061958:CDS 30.0%
!!! ACAATTTTTTGGTATGTGGA+AGG - chr6.2:20956607-20956626 MS.gene061958:CDS 30.0%
!!! GAGTACAATTTTTTGGTATG+TGG - chr6.2:20956603-20956622 MS.gene061958:CDS 30.0%
ATTATGATCGTGCTTTGCAA+TGG - chr6.2:20956821-20956840 MS.gene061958:CDS 35.0%
CGTTATAACTTCAAATCTGG+TGG - chr6.2:20956862-20956881 MS.gene061958:CDS 35.0%
TTATGATCGTGCTTTGCAAT+GGG - chr6.2:20956822-20956841 MS.gene061958:CDS 35.0%
! ACAACTGTATCTGGTTTCAA+TGG + chr6.2:20956667-20956686 None:intergenic 35.0%
AAACTATGAGTGCACTTGGT+GGG - chr6.2:20956743-20956762 MS.gene061958:CDS 40.0%
GAAGAAACTATGAGTGCACT+TGG - chr6.2:20956739-20956758 MS.gene061958:CDS 40.0%
! TTTTGGTATGTGGAAGGACT+TGG - chr6.2:20956613-20956632 MS.gene061958:CDS 40.0%
!! TGGGTCAGGTATTGAAGTAA+TGG - chr6.2:20956762-20956781 MS.gene061958:CDS 40.0%
GAAACTATGAGTGCACTTGG+TGG - chr6.2:20956742-20956761 MS.gene061958:CDS 45.0%
!! GAAGGACTTGGTGTGAATTG+GGG - chr6.2:20956625-20956644 MS.gene061958:CDS 45.0%
!! GGAAGGACTTGGTGTGAATT+GGG - chr6.2:20956624-20956643 MS.gene061958:CDS 45.0%
!! TGGAAGGACTTGGTGTGAAT+TGG - chr6.2:20956623-20956642 MS.gene061958:CDS 45.0%
! ATGAGTGCACTTGGTGGGTC+AGG - chr6.2:20956748-20956767 MS.gene061958:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 20956589 20956900 20956589 ID=MS.gene061958
chr6.2 mRNA 20956589 20956900 20956589 ID=MS.gene061958.t1;Parent=MS.gene061958
chr6.2 exon 20956589 20956900 20956589 ID=MS.gene061958.t1.exon1;Parent=MS.gene061958.t1
chr6.2 CDS 20956589 20956900 20956589 ID=cds.MS.gene061958.t1;Parent=MS.gene061958.t1
Gene Sequence

>MS.gene061958

ATGTTTATGATGATGAGTACAATTTTTTGGTATGTGGAAGGACTTGGTGTGAATTGGGGAACACAAGCAACACATCCATTGAAACCAGATACAGTTGTACAAATGTTGAAAGATAATGGAATTGAAAAAGTGAAGTTATTTGATGCTGATGAAGAAACTATGAGTGCACTTGGTGGGTCAGGTATTGAAGTAATGGTTGCTATTCCTAATAATCAATTAGCTGAAATGAGTTATTATGATCGTGCTTTGCAATGGGTTAGAAAGAATGTTACTCGTTATAACTTCAAATCTGGTGGAGTAAATATCAAGTAA

Protein sequence

>MS.gene061958.t1

MFMMMSTIFWYVEGLGVNWGTQATHPLKPDTVVQMLKDNGIEKVKLFDADEETMSALGGSGIEVMVAIPNNQLAEMSYYDRALQWVRKNVTRYNFKSGGVNIK