AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37213


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTCAAGATTTGTTA+AGG + 42363:33177-33196 None:intergenic 15.0%
!!! AAACAGTTTTCATAAATTTT+TGG - 42363:32026-32045 MS.gene37213:CDS 15.0%
!!! AACAGTTTTCATAAATTTTT+GGG - 42363:32027-32046 MS.gene37213:CDS 15.0%
!!! ACAAATCTTGAAAATTTTAA+AGG - 42363:33179-33198 MS.gene37213:intron 15.0%
!!! ATTTTTTTGTATTTACACAA+AGG - 42363:33690-33709 MS.gene37213:CDS 15.0%
!!! GTATTTTTTTTTCTTTTTTC+AGG - 42363:32776-32795 MS.gene37213:intron 15.0%
!!! GTTTATTATTTACTTTTTGA+GGG - 42363:33649-33668 MS.gene37213:CDS 15.0%
!!! TGTTTATTATTTACTTTTTG+AGG - 42363:33648-33667 MS.gene37213:CDS 15.0%
!!! TTTTTTTGTATTTACACAAA+GGG - 42363:33691-33710 MS.gene37213:CDS 15.0%
!! AAAAAATACATAAGCATGTT+TGG + 42363:32765-32784 None:intergenic 20.0%
! AAATGATTAACTAAGAAGGA+TGG + 42363:32386-32405 None:intergenic 25.0%
! AAGGAAGAAATGAACTTAAA+TGG - 42363:33293-33312 MS.gene37213:intron 25.0%
! ACCATTATTGTTCTATGAAA+AGG - 42363:33055-33074 MS.gene37213:intron 25.0%
! ATAACAATGTAACATGCAAA+TGG - 42363:31725-31744 MS.gene37213:intron 25.0%
! ATTTAGTCTCAAATTTACTG+TGG - 42363:32627-32646 MS.gene37213:intron 25.0%
! CAAATGTCATATATTTCAGA+CGG - 42363:32835-32854 MS.gene37213:intron 25.0%
! CATGAAATTATATGTAGATG+AGG - 42363:32959-32978 MS.gene37213:intron 25.0%
! CATGTTACATTGTTATAGTA+AGG + 42363:31721-31740 None:intergenic 25.0%
! CCATTATTGTTCTATGAAAA+GGG - 42363:33056-33075 MS.gene37213:intron 25.0%
! CTAAATTAACCCATCTAAAT+TGG + 42363:32153-32172 None:intergenic 25.0%
! TCACTATTATGGTTACAAAA+TGG - 42363:33130-33149 MS.gene37213:intron 25.0%
! TGTGAAATGATTAACTAAGA+AGG + 42363:32390-32409 None:intergenic 25.0%
! TTTACAATGATTGTGTTCAT+TGG - 42363:33486-33505 MS.gene37213:CDS 25.0%
!! AATTGAGTTGCATTAAACAA+CGG + 42363:31838-31857 None:intergenic 25.0%
!!! GTTTTCATAAATTTTTGGGT+TGG - 42363:32031-32050 MS.gene37213:CDS 25.0%
!!! GTTTTTTTTTTCAAAAGAGC+AGG - 42363:31981-32000 MS.gene37213:CDS 25.0%
!!! TTAAAGGTGTGACATTTTTT+AGG - 42363:33195-33214 MS.gene37213:intron 25.0%
AAACCAAACATTCACTAAGA+TGG + 42363:32467-32486 None:intergenic 30.0%
AATCCATCTTAGTGAATGTT+TGG - 42363:32461-32480 MS.gene37213:intron 30.0%
ATAGCATGTCCATAGTTATT+AGG + 42363:33449-33468 None:intergenic 30.0%
ATGAAAAAACCTTGATGTCA+CGG + 42363:32233-32252 None:intergenic 30.0%
ATGGTTACAAAATGGCATTT+AGG - 42363:33138-33157 MS.gene37213:intron 30.0%
CAAGTTAGAATCAATTCTAG+AGG + 42363:32573-32592 None:intergenic 30.0%
GATCATTACTTACTTGACTT+AGG + 42363:31942-31961 None:intergenic 30.0%
GTATTTATGAGAAACATCCT+CGG + 42363:32811-32830 None:intergenic 30.0%
TAATAACTATGGACATGCTA+TGG - 42363:33448-33467 MS.gene37213:intron 30.0%
TAGCATGTCCATAGTTATTA+GGG + 42363:33448-33467 None:intergenic 30.0%
TATGAAAAGGGTAAACTTGT+TGG - 42363:33068-33087 MS.gene37213:intron 30.0%
TATTTATGAGAAACATCCTC+GGG + 42363:32810-32829 None:intergenic 30.0%
TCTAACTTGAAGCTAGAATT+CGG - 42363:32584-32603 MS.gene37213:intron 30.0%
TTCATTTCTTCCTTGCTAAA+TGG + 42363:33287-33306 None:intergenic 30.0%
TTGTATTTACACAAAGGGTA+GGG - 42363:33696-33715 MS.gene37213:CDS 30.0%
TTTGTATTTACACAAAGGGT+AGG - 42363:33695-33714 MS.gene37213:CDS 30.0%
! CCCTTTTCATAGAACAATAA+TGG + 42363:33059-33078 None:intergenic 30.0%
! GGAAAGAAAATGGTTTTTGT+TGG - 42363:31874-31893 MS.gene37213:intron 30.0%
! TGAATGTTTGGTTTCACATT+TGG - 42363:32473-32492 MS.gene37213:intron 30.0%
! TGAATTTAAAGTAGCACAAG+AGG - 42363:33349-33368 MS.gene37213:intron 30.0%
! TTTTTGTTGGAGATTCTGTT+GGG - 42363:31887-31906 MS.gene37213:intron 30.0%
AAAACTGCATGAAGTTTGGT+AGG - 42363:31761-31780 MS.gene37213:intron 35.0%
AACTCATTCCATGTATCAAC+TGG + 42363:33524-33543 None:intergenic 35.0%
AAGAAGGATGGATACAATCA+AGG + 42363:32374-32393 None:intergenic 35.0%
AATTGGATTCCTCATTCTGA+AGG - 42363:31694-31713 MS.gene37213:intron 35.0%
ACAGATCTTGCTCACTATTA+TGG - 42363:33119-33138 MS.gene37213:intron 35.0%
ACCAAGGGTGAAATTGTAAT+CGG + 42363:32871-32890 None:intergenic 35.0%
ACCATACTTTGTGAGATCAA+AGG - 42363:32902-32921 MS.gene37213:intron 35.0%
AGAAGGATGGATACAATCAA+GGG + 42363:32373-32392 None:intergenic 35.0%
AGGGAACAAGGATAAAAACA+AGG + 42363:32349-32368 None:intergenic 35.0%
ATTATCTAAGAATGCAACCG+TGG - 42363:32310-32329 MS.gene37213:intron 35.0%
ATTATGTTGTTGAGACCTGA+TGG - 42363:33419-33438 MS.gene37213:intron 35.0%
CATAAGCATGTTTGGTTATC+TGG + 42363:32757-32776 None:intergenic 35.0%
CATTACTTACTTGACTTAGG+AGG + 42363:31939-31958 None:intergenic 35.0%
GAACAAAACTGCATGAAGTT+TGG - 42363:31757-31776 MS.gene37213:intron 35.0%
GGTTGCATTCTTAGATAATG+CGG + 42363:32309-32328 None:intergenic 35.0%
GTACAAAGTCACACATCTTA+TGG - 42363:33563-33582 MS.gene37213:CDS 35.0%
TCAAAGGATGCTGACAAAAA+TGG - 42363:32918-32937 MS.gene37213:intron 35.0%
TCCTTTGATCTCACAAAGTA+TGG + 42363:32906-32925 None:intergenic 35.0%
TCTCCATTCTCAAAATGTGA+TGG + 42363:33230-33249 None:intergenic 35.0%
TGTATTTACACAAAGGGTAG+GGG - 42363:33697-33716 MS.gene37213:CDS 35.0%
! AAAGGATGTGACATCTTTTC+TGG - 42363:31670-31689 MS.gene37213:CDS 35.0%
! ATGTTTGGTTTCACATTTGG+AGG - 42363:32476-32495 MS.gene37213:intron 35.0%
! GCTTGTTAGAGGAAAGAAAA+TGG - 42363:31864-31883 MS.gene37213:intron 35.0%
! GGAGGAAAAATTGATTCTAG+AGG - 42363:32497-32516 MS.gene37213:intron 35.0%
! GGTTATCTGGTGTCAAAAAT+TGG + 42363:32744-32763 None:intergenic 35.0%
! GTTTTTGTTGGAGATTCTGT+TGG - 42363:31886-31905 MS.gene37213:intron 35.0%
!! CAATTCTTAGAGCTTGTTAG+AGG - 42363:31853-31872 MS.gene37213:intron 35.0%
!! CACATTTTGAGAATGGAGAT+TGG - 42363:33231-33250 MS.gene37213:intron 35.0%
!! GTGACATCTTTTCTGGTAAT+TGG - 42363:31677-31696 MS.gene37213:intron 35.0%
!! TCTCCATCACATTTTGAGAA+TGG - 42363:33224-33243 MS.gene37213:intron 35.0%
!!! TTTTCCTCACTGATTTGTGT+TGG - 42363:32254-32273 MS.gene37213:CDS 35.0%
AATGGAGATTGGAACAAAGG+AGG - 42363:33242-33261 MS.gene37213:intron 40.0%
ATGAGTATCTTCGTTGGAGA+TGG - 42363:31791-31810 MS.gene37213:intron 40.0%
ATGGAGATTGGAACAAAGGA+GGG - 42363:33243-33262 MS.gene37213:intron 40.0%
CTTGTTGGATGCAACAAATG+TGG - 42363:33083-33102 MS.gene37213:intron 40.0%
GACATAGTGATCATTTCAGC+AGG - 42363:33017-33036 MS.gene37213:intron 40.0%
GAGAATGGAGATTGGAACAA+AGG - 42363:33239-33258 MS.gene37213:intron 40.0%
GAGGACAAAACCATTTAGCA+AGG - 42363:33274-33293 MS.gene37213:intron 40.0%
GATGGACACCCTAATAACTA+TGG - 42363:33437-33456 MS.gene37213:intron 40.0%
GATGGATACAATCAAGGGAA+GGG + 42363:32368-32387 None:intergenic 40.0%
TAACCCATCTAAATTGGCAC+CGG + 42363:32147-32166 None:intergenic 40.0%
TAGATGAGGTAGATGAAGCA+TGG - 42363:32973-32992 MS.gene37213:intron 40.0%
TAGTAAGGTCCTTCAGAATG+AGG + 42363:31706-31725 None:intergenic 40.0%
TGATCATTTCAGCAGGACAT+TGG - 42363:33024-33043 MS.gene37213:intron 40.0%
TGGATGCAACAAATGTGGAA+TGG - 42363:33088-33107 MS.gene37213:intron 40.0%
! ATTTCACCCTTGGTAATCTG+TGG - 42363:32877-32896 MS.gene37213:intron 40.0%
! TAGTTATTAGGGTGTCCATC+AGG + 42363:33437-33456 None:intergenic 40.0%
! TGTGTTCATTGGTGTTTACC+TGG - 42363:33497-33516 MS.gene37213:CDS 40.0%
! TTTGGTTTCACATTTGGAGG+AGG - 42363:32479-32498 MS.gene37213:intron 40.0%
!! CAAGAGGAAGCATCAAAGAA+AGG - 42363:33365-33384 MS.gene37213:intron 40.0%
!!! AAGGTTTTAAATCGCAGCCA+CGG + 42363:32330-32349 None:intergenic 40.0%
!!! CATAAATTTTTGGGTTGGCC+TGG - 42363:32036-32055 MS.gene37213:CDS 40.0%
AACCCATCTAAATTGGCACC+GGG + 42363:32146-32165 None:intergenic 45.0%
AACGGAAGCTCGCATTCATT+TGG + 42363:31820-31839 None:intergenic 45.0%
ACAAAGGAGGGAATTGTGTG+AGG - 42363:33255-33274 MS.gene37213:intron 45.0%
ACAATCAAGGGAAGGGAACA+AGG + 42363:32361-32380 None:intergenic 45.0%
AGATCGAACCTGATAACTCG+AGG + 42363:32095-32114 None:intergenic 45.0%
AGTCTCAAACCAATACCACC+AGG + 42363:32057-32076 None:intergenic 45.0%
ATGGTGACCACAGATTACCA+AGG + 42363:32887-32906 None:intergenic 45.0%
CAACGAAGATACTCATCGTC+CGG + 42363:31787-31806 None:intergenic 45.0%
GCCGATTACAATTTCACCCT+TGG - 42363:32867-32886 MS.gene37213:intron 45.0%
GGATGGATACAATCAAGGGA+AGG + 42363:32369-32388 None:intergenic 45.0%
TGGTGACCACAGATTACCAA+GGG + 42363:32886-32905 None:intergenic 45.0%
TTCCATGTATCAACTGGTCC+AGG + 42363:33518-33537 None:intergenic 45.0%
! TACCTGGACCAGTTGATACA+TGG - 42363:33513-33532 MS.gene37213:CDS 45.0%
!!! AAATTTTTGGGTTGGCCTGG+TGG - 42363:32039-32058 MS.gene37213:CDS 45.0%
ACCCATCTAAATTGGCACCG+GGG + 42363:32145-32164 None:intergenic 50.0%
CGGACGATGAGTATCTTCGT+TGG - 42363:31785-31804 MS.gene37213:intron 50.0%
CTGCATGAAGTTTGGTAGGC+CGG - 42363:31765-31784 MS.gene37213:intron 50.0%
GAATCGAACCTGATACCTCG+AGG + 42363:32121-32140 None:intergenic 50.0%
GCGACCAACACAAATCAGTG+AGG + 42363:32261-32280 None:intergenic 50.0%
GTATCAGGTTCGATTCTCCC+CGG - 42363:32125-32144 MS.gene37213:CDS 50.0%
TCGAACCTGATAACTCGAGG+AGG + 42363:32092-32111 None:intergenic 50.0%
! TCCCCGGTGCCAATTTAGAT+GGG - 42363:32141-32160 MS.gene37213:CDS 50.0%
CGACTTGAGCCGTGACATCA+AGG - 42363:32221-32240 MS.gene37213:CDS 55.0%
GTGCTCCTCCTCGAGTTATC+AGG - 42363:32084-32103 MS.gene37213:CDS 55.0%
TCGAACCTGATACCTCGAGG+AGG + 42363:32118-32137 None:intergenic 55.0%
! CTCCCCGGTGCCAATTTAGA+TGG - 42363:32140-32159 MS.gene37213:CDS 55.0%
! GATCTCCTCCTCGAGGTATC+AGG - 42363:32110-32129 MS.gene37213:CDS 55.0%
!! TTGGGTTGGCCTGGTGGTAT+TGG - 42363:32045-32064 MS.gene37213:CDS 55.0%
!! TTTTCAGGCGTCTCAACCCG+AGG - 42363:32791-32810 MS.gene37213:intron 55.0%
CACGGCTCAAGTCGCAGTCA+CGG + 42363:32215-32234 None:intergenic 60.0%
CAGGTTCGATCTCCTCCTCG+AGG - 42363:32103-32122 MS.gene37213:CDS 60.0%


Chromosome Type Strat End Strand Name
42363 gene 31665 33738 31665 ID=MS.gene37213
42363 mRNA 31665 33738 31665 ID=MS.gene37213.t1;Parent=MS.gene37213
42363 exon 33455 33738 33455 ID=MS.gene37213.t1.exon1;Parent=MS.gene37213.t1
42363 CDS 33455 33738 33455 ID=cds.MS.gene37213.t1;Parent=MS.gene37213.t1
42363 exon 31934 32326 31934 ID=MS.gene37213.t1.exon2;Parent=MS.gene37213.t1
42363 CDS 31934 32326 31934 ID=cds.MS.gene37213.t1;Parent=MS.gene37213.t1
42363 exon 31665 31691 31665 ID=MS.gene37213.t1.exon3;Parent=MS.gene37213.t1
42363 CDS 31665 31691 31665 ID=cds.MS.gene37213.t1;Parent=MS.gene37213.t1
Gene Sequence

>MS.gene37213

AAACAAAAGGATGTGACATCTTTTCTGGTAATTGGATTCCTCATTCTGAAGGACCTTACTATAACAATGTAACATGCAAATGGATGATTGATGAACAAAACTGCATGAAGTTTGGTAGGCCGGACGATGAGTATCTTCGTTGGAGATGGAAACCAAATGAATGCGAGCTTCCGTTGTTTAATGCAACTCAATTCTTAGAGCTTGTTAGAGGAAAGAAAATGGTTTTTGTTGGAGATTCTGTTGGGAGAAATCAGTTGCAATCTTTGTTGTGCCTCCTAAGTCAAGTAAGTAATGATCAACTCATACTGTTTTCTCTGTTTTTTTTTTCAAAAGAGCAGGAGAATTTTTTTTTGTTTACATCAAACAGTTTTCATAAATTTTTGGGTTGGCCTGGTGGTATTGGTTTGAGACTTGAGAGTGTGCTCCTCCTCGAGTTATCAGGTTCGATCTCCTCCTCGAGGTATCAGGTTCGATTCTCCCCGGTGCCAATTTAGATGGGTTAATTTAGTTTCTTCAAAAAAAGTTTTCATAACATAGTATACTATGTCCGTGACTGCGACTTGAGCCGTGACATCAAGGTTTTTTCATGTTTTCCTCACTGATTTGTGTTGGTCGCGTCAATCGTATTTGATTGTGATTCTCCGCATTATCTAAGAATGCAACCGTGGCTGCGATTTAAAACCTTGTTTTTATCCTTGTTCCCTTCCCTTGATTGTATCCATCCTTCTTAGTTAATCATTTCACAAATGTTTACACAAATTTTTGTTGATGATCTTGTCTGCATAGTTATATTGCAAATCCATCTTAGTGAATGTTTGGTTTCACATTTGGAGGAGGAAAAATTGATTCTAGAGGTGTAAAATTAATTTTGACATGCTTCGATGTTTTAAAGTAAATTGATTTTGCCTCTAGAATTGATTCTAACTTGAAGCTAGAATTCGGAACTTTTGACTCTACGATTAATTTAGTCTCAAATTTACTGTGGAACTCACTTTTTCATTTATATATCTAAAATCAATTTACATTCAACTCACTTTTAGCTAGAATCTATTTGACAAAATCAATTATATACAAAACCAATTTTTGACACCAGATAACCAAACATGCTTATGTATTTTTTTTTCTTTTTTCAGGCGTCTCAACCCGAGGATGTTTCTCATAAATACACAACAAATGTCATATATTTCAGACGGTATTTCTTCGCCGATTACAATTTCACCCTTGGTAATCTGTGGTCACCATACTTTGTGAGATCAAAGGATGCTGACAAAAATGGTCATTCATACAACAGCATCATGAAATTATATGTAGATGAGGTAGATGAAGCATGGTCTAGTCAAGTTAAAAACTTTGACATAGTGATCATTTCAGCAGGACATTGGTTTTTTAGACCATTATTGTTCTATGAAAAGGGTAAACTTGTTGGATGCAACAAATGTGGAATGGAGAATGTAACAGATCTTGCTCACTATTATGGTTACAAAATGGCATTTAGGACTGCATTTAAAACCTTAACAAATCTTGAAAATTTTAAAGGTGTGACATTTTTTAGGACATTTTCTCCATCACATTTTGAGAATGGAGATTGGAACAAAGGAGGGAATTGTGTGAGGACAAAACCATTTAGCAAGGAAGAAATGAACTTAAATGGTTACATTCTTGAGACATATTTAACACAAGTGAATGAATTTAAAGTAGCACAAGAGGAAGCATCAAAGAAAGGTTTGAAATTTTTGATGTTAAATACAACTGAAATTATGTTGTTGAGACCTGATGGACACCCTAATAACTATGGACATGCTATGGTAAAAAATGTGACATTTTACAATGATTGTGTTCATTGGTGTTTACCTGGACCAGTTGATACATGGAATGAGTTTTTGCTATATATGTTGAATGTACAAAGTCACACATCTTATGGTTCAAAGCTAGAGAGTGTTTTATGAAAATAAGATTTACATATGTTAACTATATTGAAGAGATTGTTTATTATTTACTTTTTGAGGGTTTGCTTACTCTATTGTAATTTTTTTGTATTTACACAAAGGGTAGGGGAAATGATGAAAGAGATTGA

Protein sequence

>MS.gene37213.t1

KQKDVTSFLVIGFLILKDLTITMUHANGULMNKTAUSLVGRTMSIFVGDGNQMNASFRCLMQLNSUSLLEERKWFLLEILLGEISCNLCCASUVKVNLLDATNVEWRMUQILLTIMVTKWHLGLHLKPUQILKILKVUHFLGHFLHHILRMEIGTKEGIVUGQNHLARKKUTUMVTFLRHIUHKUMNLKUHKRKHQRKVUNFUCUIQLKLCCUDLMDTLITMDMLWVGEMMKEI