Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39160.t1 | XP_013467705.1 | 95.1 | 265 | 10 | 2 | 1 | 265 | 1 | 262 | 3.70E-99 | 371.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39160.t1 | Q84WI0 | 41.8 | 285 | 114 | 11 | 1 | 265 | 1 | 253 | 2.4e-22 | 107.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39160.t1 | A0A072VIV1 | 95.1 | 265 | 10 | 2 | 1 | 265 | 1 | 262 | 2.7e-99 | 371.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene39160 | 0.827276 | 1.71E-54 | -1.69E-46 |
| MS.gene049173 | MS.gene39160 | 0.821591 | 3.72E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene39160 | 0.856538 | 3.01E-62 | -1.69E-46 |
| MS.gene049222 | MS.gene39160 | 0.801794 | 7.61E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene39160 | 0.809124 | 2.21E-50 | -1.69E-46 |
| MS.gene049351 | MS.gene39160 | 0.813915 | 2.01E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene39160 | 0.80265 | 5.07E-49 | -1.69E-46 |
| MS.gene049491 | MS.gene39160 | 0.805788 | 1.13E-49 | -1.69E-46 |
| MS.gene049609 | MS.gene39160 | 0.813319 | 2.72E-51 | -1.69E-46 |
| MS.gene049614 | MS.gene39160 | 0.812649 | 3.81E-51 | -1.69E-46 |
| MS.gene049760 | MS.gene39160 | 0.803294 | 3.73E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene39160 | 0.832328 | 1.01E-55 | -1.69E-46 |
| MS.gene049907 | MS.gene39160 | 0.804115 | 2.52E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene39160 | 0.82104 | 4.99E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene39160 | 0.810555 | 1.09E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene39160 | 0.81611 | 6.55E-52 | -1.69E-46 |
| MS.gene050475 | MS.gene39160 | -0.819995 | 8.66E-53 | -1.69E-46 |
| MS.gene050520 | MS.gene39160 | 0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene050541 | MS.gene39160 | -0.817574 | 3.07E-52 | -1.69E-46 |
| MS.gene050686 | MS.gene39160 | 0.8231 | 1.66E-53 | -1.69E-46 |
| MS.gene050767 | MS.gene39160 | 0.827766 | 1.31E-54 | -1.69E-46 |
| MS.gene050770 | MS.gene39160 | 0.830692 | 2.55E-55 | -1.69E-46 |
| MS.gene050772 | MS.gene39160 | 0.830091 | 3.57E-55 | -1.69E-46 |
| MS.gene05100 | MS.gene39160 | 0.810878 | 9.26E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene39160 | 0.842784 | 2.09E-58 | -1.69E-46 |
| MS.gene051228 | MS.gene39160 | 0.810307 | 1.23E-50 | -1.69E-46 |
| MS.gene051255 | MS.gene39160 | 0.805593 | 1.24E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene39160 | 0.805205 | 1.49E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene39160 | 0.825317 | 5.01E-54 | -1.69E-46 |
| MS.gene051692 | MS.gene39160 | 0.829026 | 6.48E-55 | -1.69E-46 |
| MS.gene051810 | MS.gene39160 | 0.831018 | 2.12E-55 | -1.69E-46 |
| MS.gene051858 | MS.gene39160 | 0.855935 | 4.52E-62 | -1.69E-46 |
| MS.gene051877 | MS.gene39160 | 0.804812 | 1.81E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene39160 | 0.808253 | 3.39E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene39160 | 0.879053 | 1.71E-69 | -1.69E-46 |
| MS.gene052290 | MS.gene39160 | 0.813337 | 2.69E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene39160 | 0.805368 | 1.38E-49 | -1.69E-46 |
| MS.gene052322 | MS.gene39160 | 0.802843 | 4.63E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene39160 | 0.831469 | 1.64E-55 | -1.69E-46 |
| MS.gene052421 | MS.gene39160 | 0.824119 | 9.59E-54 | -1.69E-46 |
| MS.gene052481 | MS.gene39160 | 0.810673 | 1.03E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene39160 | 0.837585 | 4.76E-57 | -1.69E-46 |
| MS.gene052485 | MS.gene39160 | 0.823406 | 1.41E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene39160 | 0.837885 | 3.99E-57 | -1.69E-46 |
| MS.gene053009 | MS.gene39160 | 0.834983 | 2.19E-56 | -1.69E-46 |
| MS.gene053103 | MS.gene39160 | 0.822607 | 2.16E-53 | -1.69E-46 |
| MS.gene053104 | MS.gene39160 | 0.806401 | 8.38E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene39160 | 0.841343 | 5.03E-58 | -1.69E-46 |
| MS.gene053209 | MS.gene39160 | 0.818795 | 1.63E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene39160 | 0.810292 | 1.24E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene39160 | 0.856755 | 2.60E-62 | -1.69E-46 |
| MS.gene053449 | MS.gene39160 | 0.808873 | 2.50E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene39160 | 0.854992 | 8.50E-62 | -1.69E-46 |
| MS.gene053813 | MS.gene39160 | 0.860583 | 1.87E-63 | -1.69E-46 |
| MS.gene053915 | MS.gene39160 | 0.817422 | 3.33E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene39160 | 0.808691 | 2.74E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene39160 | 0.802826 | 4.67E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene39160 | 0.837664 | 4.55E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene39160 | 0.817648 | 2.96E-52 | -1.69E-46 |
| MS.gene054802 | MS.gene39160 | 0.824287 | 8.76E-54 | -1.69E-46 |
| MS.gene054803 | MS.gene39160 | 0.819953 | 8.86E-53 | -1.69E-46 |
| MS.gene054804 | MS.gene39160 | 0.826209 | 3.08E-54 | -1.69E-46 |
| MS.gene05505 | MS.gene39160 | 0.841711 | 4.03E-58 | -1.69E-46 |
| MS.gene055206 | MS.gene39160 | 0.833274 | 5.86E-56 | -1.69E-46 |
| MS.gene055254 | MS.gene39160 | 0.813721 | 2.22E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene39160 | 0.802794 | 4.74E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene39160 | 0.800978 | 1.12E-48 | -1.69E-46 |
| MS.gene055397 | MS.gene39160 | 0.828387 | 9.25E-55 | -1.69E-46 |
| MS.gene055412 | MS.gene39160 | 0.824248 | 8.94E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene39160 | 0.832836 | 7.53E-56 | -1.69E-46 |
| MS.gene055502 | MS.gene39160 | 0.807337 | 5.31E-50 | -1.69E-46 |
| MS.gene055636 | MS.gene39160 | 0.825045 | 5.81E-54 | -1.69E-46 |
| MS.gene055638 | MS.gene39160 | 0.816167 | 6.36E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene39160 | 0.845456 | 4.02E-59 | -1.69E-46 |
| MS.gene055722 | MS.gene39160 | 0.84657 | 2.00E-59 | -1.69E-46 |
| MS.gene05592 | MS.gene39160 | 0.836319 | 1.00E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene39160 | 0.826133 | 3.21E-54 | -1.69E-46 |
| MS.gene056191 | MS.gene39160 | 0.825392 | 4.81E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene39160 | 0.833543 | 5.02E-56 | -1.69E-46 |
| MS.gene056410 | MS.gene39160 | 0.829047 | 6.41E-55 | -1.69E-46 |
| MS.gene056449 | MS.gene39160 | 0.816737 | 4.74E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene39160 | 0.826126 | 3.22E-54 | -1.69E-46 |
| MS.gene056611 | MS.gene39160 | 0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene39160 | 0.809511 | 1.83E-50 | -1.69E-46 |
| MS.gene056942 | MS.gene39160 | 0.817208 | 3.72E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene39160 | 0.810109 | 1.36E-50 | -1.69E-46 |
| MS.gene057123 | MS.gene39160 | 0.800959 | 1.13E-48 | -1.69E-46 |
| MS.gene057129 | MS.gene39160 | 0.861768 | 8.14E-64 | -1.69E-46 |
| MS.gene057146 | MS.gene39160 | 0.831065 | 2.06E-55 | -1.69E-46 |
| MS.gene057285 | MS.gene39160 | 0.807579 | 4.72E-50 | -1.69E-46 |
| MS.gene057295 | MS.gene39160 | 0.812918 | 3.33E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene39160 | 0.834925 | 2.26E-56 | -1.69E-46 |
| MS.gene057749 | MS.gene39160 | 0.81348 | 2.51E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene39160 | 0.824694 | 7.03E-54 | -1.69E-46 |
| MS.gene057926 | MS.gene39160 | 0.805779 | 1.13E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene39160 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene39160 | 0.815127 | 1.08E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene39160 | 0.824517 | 7.73E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene39160 | 0.840603 | 7.87E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene39160 | 0.816756 | 4.69E-52 | -1.69E-46 |
| MS.gene058903 | MS.gene39160 | 0.810082 | 1.38E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene39160 | 0.80625 | 9.01E-50 | -1.69E-46 |
| MS.gene058993 | MS.gene39160 | 0.826284 | 2.95E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene39160 | 0.803345 | 3.64E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene39160 | 0.819089 | 1.39E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene39160 | 0.807794 | 4.25E-50 | -1.69E-46 |
| MS.gene059368 | MS.gene39160 | 0.812122 | 4.97E-51 | -1.69E-46 |
| MS.gene059455 | MS.gene39160 | 0.80229 | 6.02E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene39160 | 0.838008 | 3.71E-57 | -1.69E-46 |
| MS.gene059676 | MS.gene39160 | 0.817343 | 3.46E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene39160 | 0.802707 | 4.94E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene39160 | 0.821646 | 3.61E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene39160 | 0.832932 | 7.13E-56 | -1.69E-46 |
| MS.gene059695 | MS.gene39160 | 0.83314 | 6.33E-56 | -1.69E-46 |
| MS.gene059711 | MS.gene39160 | 0.847182 | 1.36E-59 | -1.69E-46 |
| MS.gene059718 | MS.gene39160 | 0.820817 | 5.61E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene39160 | 0.853285 | 2.64E-61 | -1.69E-46 |
| MS.gene059960 | MS.gene39160 | 0.825383 | 4.83E-54 | -1.69E-46 |
| MS.gene059974 | MS.gene39160 | 0.802121 | 6.52E-49 | -1.69E-46 |
| MS.gene060356 | MS.gene39160 | 0.823385 | 1.43E-53 | -1.69E-46 |
| MS.gene060727 | MS.gene39160 | 0.802024 | 6.82E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene39160 | 0.818954 | 1.50E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene39160 | 0.843791 | 1.13E-58 | -1.69E-46 |
| MS.gene060837 | MS.gene39160 | 0.844473 | 7.40E-59 | -1.69E-46 |
| MS.gene06092 | MS.gene39160 | 0.803181 | 3.94E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene39160 | 0.824782 | 6.70E-54 | -1.69E-46 |
| MS.gene061150 | MS.gene39160 | 0.804368 | 2.23E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene39160 | 0.869881 | 2.22E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39160.t1 | MTR_1g054450 | 96.604 | 265 | 6 | 2 | 1 | 265 | 1 | 262 | 0.0 | 521 |
| MS.gene39160.t1 | MTR_3g012010 | 61.111 | 54 | 18 | 1 | 72 | 122 | 79 | 132 | 2.32e-12 | 66.2 |
| MS.gene39160.t1 | MTR_1g013130 | 44.565 | 92 | 43 | 3 | 51 | 140 | 63 | 148 | 1.18e-11 | 63.5 |
| MS.gene39160.t1 | MTR_3g106230 | 36.129 | 155 | 77 | 6 | 63 | 197 | 43 | 195 | 1.60e-11 | 62.8 |
| MS.gene39160.t1 | MTR_5g008280 | 40.426 | 94 | 48 | 3 | 78 | 167 | 36 | 125 | 1.96e-11 | 63.9 |
| MS.gene39160.t1 | MTR_3g011990 | 57.407 | 54 | 20 | 1 | 72 | 122 | 79 | 132 | 5.52e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39160.t1 | AT3G58070 | 40.071 | 282 | 123 | 13 | 1 | 265 | 1 | 253 | 1.28e-41 | 143 |
| MS.gene39160.t1 | AT2G41940 | 39.465 | 299 | 105 | 9 | 1 | 265 | 1 | 257 | 2.49e-41 | 143 |
| MS.gene39160.t1 | AT5G06650 | 35.094 | 265 | 89 | 12 | 9 | 265 | 2 | 191 | 1.08e-27 | 105 |
| MS.gene39160.t1 | AT1G68360 | 63.265 | 49 | 18 | 0 | 88 | 136 | 71 | 119 | 1.59e-14 | 71.6 |
| MS.gene39160.t1 | AT1G67030 | 44.615 | 65 | 36 | 0 | 63 | 127 | 14 | 78 | 2.43e-14 | 70.1 |
| MS.gene39160.t1 | AT1G24625 | 35.965 | 114 | 55 | 3 | 44 | 157 | 16 | 111 | 1.94e-11 | 62.4 |
Find 53 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGGAAAGATCAAATTTA+TGG | 0.180908 | 1.2:+41879500 | MS.gene39160:CDS |
| TTGTTGTTGTTGGTTGTTTC+CGG | 0.244675 | 1.2:-41878995 | None:intergenic |
| TCTCAACAACAACCTATTAA+TGG | 0.266540 | 1.2:+41879388 | MS.gene39160:CDS |
| TACTCTGAACTGTGCCGTTT+GGG | 0.291886 | 1.2:-41879435 | None:intergenic |
| GCTAAAGGACTACCATTAAT+AGG | 0.319842 | 1.2:-41879400 | None:intergenic |
| GCGGATGCCTTTCTCTTTGT+TGG | 0.329503 | 1.2:-41878918 | None:intergenic |
| CGCCTCTTCGGTATCGAATT+CGG | 0.358178 | 1.2:+41878938 | MS.gene39160:CDS |
| GGTGGGAGGAGGACGGATGA+AGG | 0.394261 | 1.2:-41878897 | None:intergenic |
| TTACTCTGAACTGTGCCGTT+TGG | 0.396162 | 1.2:-41879436 | None:intergenic |
| ACGTAACGACCATGTTAGTT+TGG | 0.409802 | 1.2:+41879591 | MS.gene39160:CDS |
| ACAACATCTTCAATCAACTA+TGG | 0.410424 | 1.2:+41879192 | MS.gene39160:CDS |
| TGAGAGTAAGAAGAAGTTGT+TGG | 0.413123 | 1.2:-41879370 | None:intergenic |
| TTCTCTTTGTTGGTGGGAGG+AGG | 0.418390 | 1.2:-41878908 | None:intergenic |
| TTTACCTTTGCTTGCTTCTG+AGG | 0.423534 | 1.2:+41879483 | MS.gene39160:CDS |
| TTTCCTACTTCACAAGCCTT+AGG | 0.446992 | 1.2:+41879124 | MS.gene39160:CDS |
| GTGTTCCATGTTGGATAAGA+AGG | 0.460404 | 1.2:-41879322 | None:intergenic |
| ATGCCTTTCTCTTTGTTGGT+GGG | 0.461202 | 1.2:-41878914 | None:intergenic |
| GAGAAAGGCATCCGCCTCTT+CGG | 0.461897 | 1.2:+41878926 | MS.gene39160:CDS |
| AGACGACTCCTCCGAGTCTC+CGG | 0.464774 | 1.2:+41878976 | MS.gene39160:CDS |
| GGACGTTCATGGTTGAAACT+AGG | 0.467117 | 1.2:-41879460 | None:intergenic |
| CCACCTAAGGCTTGTGAAGT+AGG | 0.467927 | 1.2:-41879127 | None:intergenic |
| GATGCCTTTCTCTTTGTTGG+TGG | 0.472628 | 1.2:-41878915 | None:intergenic |
| GACGTTCATGGTTGAAACTA+GGG | 0.477100 | 1.2:-41879459 | None:intergenic |
| AAAGAGGATGGTGTTGTTGA+TGG | 0.481398 | 1.2:-41879258 | None:intergenic |
| CCTTTCTCTTTGTTGGTGGG+AGG | 0.482012 | 1.2:-41878911 | None:intergenic |
| GTTGGTTGTTTCCGGAGACT+CGG | 0.483985 | 1.2:-41878987 | None:intergenic |
| GCAAAGGTAAAGGACGTTCA+TGG | 0.484971 | 1.2:-41879471 | None:intergenic |
| AGACTCGGAGGAGTCGTCTT+CGG | 0.500113 | 1.2:-41878972 | None:intergenic |
| CTTTGTTGGTGGGAGGAGGA+CGG | 0.508001 | 1.2:-41878904 | None:intergenic |
| GGTGTTGTTGATGGTGATGA+TGG | 0.514460 | 1.2:-41879249 | None:intergenic |
| CATGGAACACAAACTCAACT+AGG | 0.515152 | 1.2:+41879335 | MS.gene39160:CDS |
| TTCGGTATCGAATTCGGTGG+TGG | 0.520561 | 1.2:+41878944 | MS.gene39160:CDS |
| GTGGGAGGAGGACGGATGAA+GGG | 0.532067 | 1.2:-41878896 | None:intergenic |
| GTTGAGTTTGTGTTCCATGT+TGG | 0.535384 | 1.2:-41879331 | None:intergenic |
| CCTCCCACCAACAAAGAGAA+AGG | 0.537991 | 1.2:+41878911 | MS.gene39160:CDS |
| CAACAACATTAACTCGTTCG+AGG | 0.551035 | 1.2:+41879015 | MS.gene39160:CDS |
| AATTTATGGCACAGTGGACA+AGG | 0.565618 | 1.2:+41879514 | MS.gene39160:CDS |
| CCTACTTCACAAGCCTTAGG+TGG | 0.566151 | 1.2:+41879127 | MS.gene39160:CDS |
| AGTAAGAAGAAGTTGTTGGA+TGG | 0.569777 | 1.2:-41879366 | None:intergenic |
| TCAGAAGCAAGCAAAGGTAA+AGG | 0.571923 | 1.2:-41879481 | None:intergenic |
| CATCACCTTCTTATCCAACA+TGG | 0.575046 | 1.2:+41879317 | MS.gene39160:CDS |
| TGGTGATGATGGTAGAGATG+TGG | 0.592964 | 1.2:-41879238 | None:intergenic |
| GGTGATGATGATAATGATGA+TGG | 0.595521 | 1.2:-41879301 | None:intergenic |
| CTCGGAGGAGTCGTCTTCGG+TGG | 0.604278 | 1.2:-41878969 | None:intergenic |
| GATGATGTGAATCTATAAAG+AGG | 0.609056 | 1.2:-41879274 | None:intergenic |
| ATGTGAATCTATAAAGAGGA+TGG | 0.616266 | 1.2:-41879270 | None:intergenic |
| GGTTGTTTCCGGAGACTCGG+AGG | 0.647158 | 1.2:-41878984 | None:intergenic |
| CGAATTCGATACCGAAGAGG+CGG | 0.654284 | 1.2:-41878937 | None:intergenic |
| CTTTCCTCAGAAGCAAGCAA+AGG | 0.654611 | 1.2:-41879487 | None:intergenic |
| CACCGAATTCGATACCGAAG+AGG | 0.659063 | 1.2:-41878940 | None:intergenic |
| CTCTTCGGTATCGAATTCGG+TGG | 0.666246 | 1.2:+41878941 | MS.gene39160:CDS |
| GATGAAGATCCAAACTAACA+TGG | 0.681809 | 1.2:-41879600 | None:intergenic |
| AGATCAAATTTATGGCACAG+TGG | 0.730014 | 1.2:+41879508 | MS.gene39160:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAACATCTTCAATCAACTA+TGG | + | chr1.2:41879192-41879211 | MS.gene39160:CDS | 30.0% | |
| ATGTGAATCTATAAAGAGGA+TGG | - | chr1.2:41879273-41879292 | None:intergenic | 30.0% | |
| CTGAGGAAAGATCAAATTTA+TGG | + | chr1.2:41879500-41879519 | MS.gene39160:CDS | 30.0% | |
| GATGATGTGAATCTATAAAG+AGG | - | chr1.2:41879277-41879296 | None:intergenic | 30.0% | |
| TCTCAACAACAACCTATTAA+TGG | + | chr1.2:41879388-41879407 | MS.gene39160:CDS | 30.0% | |
| ! | GTTAATGTTGTTGTTGTTGT+TGG | - | chr1.2:41879008-41879027 | None:intergenic | 30.0% |
| AGATCAAATTTATGGCACAG+TGG | + | chr1.2:41879508-41879527 | MS.gene39160:CDS | 35.0% | |
| AGTAAGAAGAAGTTGTTGGA+TGG | - | chr1.2:41879369-41879388 | None:intergenic | 35.0% | |
| GATGAAGATCCAAACTAACA+TGG | - | chr1.2:41879603-41879622 | None:intergenic | 35.0% | |
| GCTAAAGGACTACCATTAAT+AGG | - | chr1.2:41879403-41879422 | None:intergenic | 35.0% | |
| TGAGAGTAAGAAGAAGTTGT+TGG | - | chr1.2:41879373-41879392 | None:intergenic | 35.0% | |
| ! | GGTGATGATGATAATGATGA+TGG | - | chr1.2:41879304-41879323 | None:intergenic | 35.0% |
| ! | TTGTTGTTGTTGGTTGTTTC+CGG | - | chr1.2:41878998-41879017 | None:intergenic | 35.0% |
| !!! | ATGGTAGTCCTTTAGCTTTT+TGG | + | chr1.2:41879407-41879426 | MS.gene39160:CDS | 35.0% |
| AATTTATGGCACAGTGGACA+AGG | + | chr1.2:41879514-41879533 | MS.gene39160:CDS | 40.0% | |
| ACGTAACGACCATGTTAGTT+TGG | + | chr1.2:41879591-41879610 | MS.gene39160:CDS | 40.0% | |
| ATGCCTTTCTCTTTGTTGGT+GGG | - | chr1.2:41878917-41878936 | None:intergenic | 40.0% | |
| CAACAACATTAACTCGTTCG+AGG | + | chr1.2:41879015-41879034 | MS.gene39160:CDS | 40.0% | |
| CATCACCTTCTTATCCAACA+TGG | + | chr1.2:41879317-41879336 | MS.gene39160:CDS | 40.0% | |
| CATGGAACACAAACTCAACT+AGG | + | chr1.2:41879335-41879354 | MS.gene39160:CDS | 40.0% | |
| GAAGCAGAAGAAGAAGAAGA+AGG | - | chr1.2:41879544-41879563 | None:intergenic | 40.0% | |
| GACGTTCATGGTTGAAACTA+GGG | - | chr1.2:41879462-41879481 | None:intergenic | 40.0% | |
| GTGTTCCATGTTGGATAAGA+AGG | - | chr1.2:41879325-41879344 | None:intergenic | 40.0% | |
| GTTGAGTTTGTGTTCCATGT+TGG | - | chr1.2:41879334-41879353 | None:intergenic | 40.0% | |
| TCAGAAGCAAGCAAAGGTAA+AGG | - | chr1.2:41879484-41879503 | None:intergenic | 40.0% | |
| TTTACCTTTGCTTGCTTCTG+AGG | + | chr1.2:41879483-41879502 | MS.gene39160:CDS | 40.0% | |
| TTTCCTACTTCACAAGCCTT+AGG | + | chr1.2:41879124-41879143 | MS.gene39160:CDS | 40.0% | |
| !! | AAAGAGGATGGTGTTGTTGA+TGG | - | chr1.2:41879261-41879280 | None:intergenic | 40.0% |
| CTTTCCTCAGAAGCAAGCAA+AGG | - | chr1.2:41879490-41879509 | None:intergenic | 45.0% | |
| GATGCCTTTCTCTTTGTTGG+TGG | - | chr1.2:41878918-41878937 | None:intergenic | 45.0% | |
| GCAAAGGTAAAGGACGTTCA+TGG | - | chr1.2:41879474-41879493 | None:intergenic | 45.0% | |
| GGACGTTCATGGTTGAAACT+AGG | - | chr1.2:41879463-41879482 | None:intergenic | 45.0% | |
| ! | TTACTCTGAACTGTGCCGTT+TGG | - | chr1.2:41879439-41879458 | None:intergenic | 45.0% |
| !! | GGTGTTGTTGATGGTGATGA+TGG | - | chr1.2:41879252-41879271 | None:intergenic | 45.0% |
| !! | TACTCTGAACTGTGCCGTTT+GGG | - | chr1.2:41879438-41879457 | None:intergenic | 45.0% |
| !! | TGCGTTTTGATGACCACCTA+AGG | - | chr1.2:41879143-41879162 | None:intergenic | 45.0% |
| !! | TGGTGATGATGGTAGAGATG+TGG | - | chr1.2:41879241-41879260 | None:intergenic | 45.0% |
| CACCGAATTCGATACCGAAG+AGG | - | chr1.2:41878943-41878962 | None:intergenic | 50.0% | |
| CCACCTAAGGCTTGTGAAGT+AGG | - | chr1.2:41879130-41879149 | None:intergenic | 50.0% | |
| CCTACTTCACAAGCCTTAGG+TGG | + | chr1.2:41879127-41879146 | MS.gene39160:CDS | 50.0% | |
| CCTCCCACCAACAAAGAGAA+AGG | + | chr1.2:41878911-41878930 | MS.gene39160:CDS | 50.0% | |
| CGAATTCGATACCGAAGAGG+CGG | - | chr1.2:41878940-41878959 | None:intergenic | 50.0% | |
| GCGGATGCCTTTCTCTTTGT+TGG | - | chr1.2:41878921-41878940 | None:intergenic | 50.0% | |
| GGGACACGCCAAAAAGCTAA+AGG | - | chr1.2:41879418-41879437 | None:intergenic | 50.0% | |
| ! | CCTTTCTCTTTGTTGGTGGG+AGG | - | chr1.2:41878914-41878933 | None:intergenic | 50.0% |
| ! | CGCCTCTTCGGTATCGAATT+CGG | + | chr1.2:41878938-41878957 | MS.gene39160:CDS | 50.0% |
| ! | CTCTTCGGTATCGAATTCGG+TGG | + | chr1.2:41878941-41878960 | MS.gene39160:CDS | 50.0% |
| ! | GTTGGTTGTTTCCGGAGACT+CGG | - | chr1.2:41878990-41879009 | None:intergenic | 50.0% |
| ! | TTCGGTATCGAATTCGGTGG+TGG | + | chr1.2:41878944-41878963 | MS.gene39160:CDS | 50.0% |
| ! | TTCTCTTTGTTGGTGGGAGG+AGG | - | chr1.2:41878911-41878930 | None:intergenic | 50.0% |
| !! | GCTTTTTGGCGTGTCCCAAA+CGG | + | chr1.2:41879421-41879440 | MS.gene39160:CDS | 50.0% |
| AGACTCGGAGGAGTCGTCTT+CGG | - | chr1.2:41878975-41878994 | None:intergenic | 55.0% | |
| ! | CTTTGTTGGTGGGAGGAGGA+CGG | - | chr1.2:41878907-41878926 | None:intergenic | 55.0% |
| !! | GAGAAAGGCATCCGCCTCTT+CGG | + | chr1.2:41878926-41878945 | MS.gene39160:CDS | 55.0% |
| AGACGACTCCTCCGAGTCTC+CGG | + | chr1.2:41878976-41878995 | MS.gene39160:CDS | 60.0% | |
| GTGGGAGGAGGACGGATGAA+GGG | - | chr1.2:41878899-41878918 | None:intergenic | 60.0% | |
| ! | GGTTGTTTCCGGAGACTCGG+AGG | - | chr1.2:41878987-41879006 | None:intergenic | 60.0% |
| CTCGGAGGAGTCGTCTTCGG+TGG | - | chr1.2:41878972-41878991 | None:intergenic | 65.0% | |
| GGTGGGAGGAGGACGGATGA+AGG | - | chr1.2:41878900-41878919 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 41878830 | 41879627 | 41878830 | ID=MS.gene39160 |
| chr1.2 | mRNA | 41878830 | 41879627 | 41878830 | ID=MS.gene39160.t1;Parent=MS.gene39160 |
| chr1.2 | exon | 41878830 | 41879627 | 41878830 | ID=MS.gene39160.t1.exon1;Parent=MS.gene39160.t1 |
| chr1.2 | CDS | 41878830 | 41879627 | 41878830 | ID=cds.MS.gene39160.t1;Parent=MS.gene39160.t1 |
| Gene Sequence |
| Protein sequence |
