AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66702


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66702.t1 MTR_4g052010 98.404 188 3 0 1 188 1 188 1.19e-134 374
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66702.t1 AT2G27290 62.827 191 51 4 6 181 5 190 1.01e-62 193

Find 36 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCTTGGTTACTTCTTC+AGG 0.280999 4.3:-47862546 None:intergenic
ACAAAGCTGCATCTCCAATT+AGG 0.288679 4.3:+47863874 MS.gene66702:CDS
AAGGAGTTGCTGGCAAAATA+TGG 0.314060 4.3:+47863591 MS.gene66702:CDS
AGCTTCAAGACCATACTTCT+TGG 0.339383 4.3:-47862559 None:intergenic
ATGGTCTTGAAGCTGGTCTT+TGG 0.345812 4.3:+47862568 MS.gene66702:CDS
ACTGTGGGTGGAAACCTAAT+TGG 0.379765 4.3:-47863888 None:intergenic
TGATCAAGCCAAGGAGTTGC+TGG 0.407305 4.3:+47863581 MS.gene66702:CDS
CAATCCAACCAGCTACGATT+GGG 0.413841 4.3:-47863920 None:intergenic
AAAAGTCGGTACCTTTGCTT+TGG 0.429669 4.3:+47863839 MS.gene66702:CDS
GAAGAAGTAACCAAGAAGTA+TGG 0.441811 4.3:+47862549 MS.gene66702:CDS
ATTAGATTTACGTGACTGAA+TGG 0.446882 4.3:-47862451 None:intergenic
CCAATCCAACCAGCTACGAT+TGG 0.459512 4.3:-47863921 None:intergenic
TTCAGGTGAAGATTGCTTTG+AGG 0.462272 4.3:-47862529 None:intergenic
TGTTATGTATTAATCAATGC+TGG 0.464473 4.3:+47863666 MS.gene66702:CDS
CCAATCGTAGCTGGTTGGAT+TGG 0.470086 4.3:+47863921 MS.gene66702:CDS
AAGAAGTATGGTCTTGAAGC+TGG 0.499317 4.3:+47862561 MS.gene66702:CDS
TGTTCAAACTTTGCTTCAGA+AGG 0.506472 4.3:+47863695 MS.gene66702:CDS
GCTCTTACCCCAATCGTAGC+TGG 0.519207 4.3:+47863912 MS.gene66702:CDS
GGGGAACAACAGAAATCAAA+AGG 0.520589 4.3:+47863558 MS.gene66702:CDS
TTACCCCAATCGTAGCTGGT+TGG 0.540207 4.3:+47863916 MS.gene66702:CDS
TTTCAGTTCAAAGGAAGAAG+GGG 0.549822 4.3:+47863539 MS.gene66702:CDS
AGAGATCAATGACAGAGTAA+TGG 0.561630 4.3:-47863634 None:intergenic
TCTTGAAGCTGGTCTTTGGA+AGG 0.564484 4.3:+47862572 MS.gene66702:CDS
AAAGAAGCACAAGAAGGTGA+TGG 0.588002 4.3:-47862387 None:intergenic
CTCAGAAAAGAAGCACAAGA+AGG 0.593610 4.3:-47862393 None:intergenic
ATCAAAAGGTGATCAAGCCA+AGG 0.614436 4.3:+47863572 MS.gene66702:CDS
GGGGTAAGAGCTACTGTGGG+TGG 0.621082 4.3:-47863900 None:intergenic
GATCAATGACAGAGTAATGG+AGG 0.626103 4.3:-47863631 None:intergenic
CAATGACAGAGTAATGGAGG+TGG 0.627479 4.3:-47863628 None:intergenic
GGAATCACTACTGATGCTAC+CGG 0.632753 4.3:+47863813 MS.gene66702:CDS
TGCAGCATATGCCAAAGCAA+AGG 0.641846 4.3:-47863850 None:intergenic
GATTGGGGTAAGAGCTACTG+TGG 0.646696 4.3:-47863904 None:intergenic
AATCCAACCAGCTACGATTG+GGG 0.648062 4.3:-47863919 None:intergenic
GAGTTGCTGGCAAAATATGG+AGG 0.682334 4.3:+47863594 MS.gene66702:CDS
ATTGGGGTAAGAGCTACTGT+GGG 0.725389 4.3:-47863903 None:intergenic
AGCTGTTAAAGAGAAAACTG+AGG 0.740712 4.3:+47862494 MS.gene66702:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGAAGGAAAAAAAAA+AGG - chr4.3:47862735-47862754 None:intergenic 15.0%
!! AAATAAATAAGAGAAAGAAA+TGG - chr4.3:47862601-47862620 None:intergenic 15.0%
!! AATAAATAAGAGAAAGAAAT+GGG - chr4.3:47862600-47862619 None:intergenic 15.0%
!! ATTATACTTAAATCTACATT+GGG - chr4.3:47863739-47863758 None:intergenic 15.0%
!!! ATTTAAAGCAATAAAAATGA+AGG - chr4.3:47862747-47862766 None:intergenic 15.0%
!!! GTTCTAAAAAGTATATATAA+AGG + chr4.3:47862800-47862819 MS.gene66702:intron 15.0%
!!! TTTCTTTCTCTTATTTATTT+TGG + chr4.3:47862601-47862620 MS.gene66702:intron 15.0%
!! CATTATACTTAAATCTACAT+TGG - chr4.3:47863740-47863759 None:intergenic 20.0%
!! TATAAAGGATATTCAAAGAT+AGG + chr4.3:47862815-47862834 MS.gene66702:intron 20.0%
!! TCTGTGTAAAATGAAAATTA+TGG + chr4.3:47863504-47863523 MS.gene66702:intron 20.0%
!! TTCAAACTTCTACTTTAAAT+TGG - chr4.3:47863199-47863218 None:intergenic 20.0%
!! TTGGTTTAATTATGATTATG+TGG + chr4.3:47862620-47862639 MS.gene66702:intron 20.0%
!!! GAAATATAAGTTGAGTTTTA+AGG + chr4.3:47863281-47863300 MS.gene66702:intron 20.0%
!!! TTGTTTACGAGTTTTATTTT+AGG + chr4.3:47863016-47863035 MS.gene66702:intron 20.0%
! AAAAGATGAATTTCTCACTT+AGG - chr4.3:47862672-47862691 None:intergenic 25.0%
! ACTAATGATTGAAAATCGTT+TGG - chr4.3:47863427-47863446 None:intergenic 25.0%
! TGTTATGTATTAATCAATGC+TGG + chr4.3:47863666-47863685 MS.gene66702:CDS 25.0%
!! AAGTGAGAAATTCATCTTTT+TGG + chr4.3:47862672-47862691 MS.gene66702:intron 25.0%
!! AGTGAGAAATTCATCTTTTT+GGG + chr4.3:47862673-47862692 MS.gene66702:intron 25.0%
!! GACATGGATTTTATACTTAT+GGG + chr4.3:47863378-47863397 MS.gene66702:intron 25.0%
!! TGACATGGATTTTATACTTA+TGG + chr4.3:47863377-47863396 MS.gene66702:intron 25.0%
!!! TAGGTTTTATTTCCATTTCT+TGG + chr4.3:47863035-47863054 MS.gene66702:intron 25.0%
ATTAGATTTACGTGACTGAA+TGG - chr4.3:47862454-47862473 None:intergenic 30.0%
TATCTCAGCAGTAAAATTGT+CGG - chr4.3:47863225-47863244 None:intergenic 30.0%
TTTCTTGTCATGTAGAACTT+GGG + chr4.3:47862930-47862949 MS.gene66702:intron 30.0%
! ATTTTATTGCCGAAATTTGC+AGG + chr4.3:47862838-47862857 MS.gene66702:intron 30.0%
! ATTTTCAGTTCAAAGGAAGA+AGG + chr4.3:47863537-47863556 MS.gene66702:CDS 30.0%
! TGTATCTTTCCCTGATTTTA+GGG + chr4.3:47863068-47863087 MS.gene66702:intron 30.0%
! TTTGCAGATTTTCAGTTCAA+AGG + chr4.3:47863530-47863549 MS.gene66702:intron 30.0%
! TTTTATTGCCGAAATTTGCA+GGG + chr4.3:47862839-47862858 MS.gene66702:intron 30.0%
! TTTTCAGTTCAAAGGAAGAA+GGG + chr4.3:47863538-47863557 MS.gene66702:CDS 30.0%
! TTTTCTTGTCATGTAGAACT+TGG + chr4.3:47862929-47862948 MS.gene66702:intron 30.0%
AGAGATCAATGACAGAGTAA+TGG - chr4.3:47863637-47863656 None:intergenic 35.0%
AGCTGTTAAAGAGAAAACTG+AGG + chr4.3:47862494-47862513 MS.gene66702:CDS 35.0%
CAGAATCGATATGCAAACAT+TGG - chr4.3:47863163-47863182 None:intergenic 35.0%
CAGTACAGAACATTTCTTGA+AGG - chr4.3:47863125-47863144 None:intergenic 35.0%
CAGTATGCTAAACCAAGAAA+TGG - chr4.3:47863050-47863069 None:intergenic 35.0%
CATTAAAACGATAACCCAAC+TGG - chr4.3:47863343-47863362 None:intergenic 35.0%
CATTATGTCTGTATATCTGC+AGG + chr4.3:47863788-47863807 MS.gene66702:intron 35.0%
GAAGAAGTAACCAAGAAGTA+TGG + chr4.3:47862549-47862568 MS.gene66702:CDS 35.0%
TACTTCTTGGTTACTTCTTC+AGG - chr4.3:47862549-47862568 None:intergenic 35.0%
TGTTCAAACTTTGCTTCAGA+AGG + chr4.3:47863695-47863714 MS.gene66702:CDS 35.0%
TTTACACAGAATGAAAAGCG+CGG - chr4.3:47863494-47863513 None:intergenic 35.0%
TTTCAGTTCAAAGGAAGAAG+GGG + chr4.3:47863539-47863558 MS.gene66702:CDS 35.0%
TTTCCTTTCCCTGCAAATTT+CGG - chr4.3:47862850-47862869 None:intergenic 35.0%
! ATTTTGCTCCCCTAAAATCA+GGG - chr4.3:47863080-47863099 None:intergenic 35.0%
! CAGTTGGGTTATCGTTTTAA+TGG + chr4.3:47863341-47863360 MS.gene66702:intron 35.0%
! CTGTATCTTTCCCTGATTTT+AGG + chr4.3:47863067-47863086 MS.gene66702:intron 35.0%
! GTATCTTTCCCTGATTTTAG+GGG + chr4.3:47863069-47863088 MS.gene66702:intron 35.0%
! TATTTTGCTCCCCTAAAATC+AGG - chr4.3:47863081-47863100 None:intergenic 35.0%
AAAAGTCGGTACCTTTGCTT+TGG + chr4.3:47863839-47863858 MS.gene66702:CDS 40.0%
AAGGAGTTGCTGGCAAAATA+TGG + chr4.3:47863591-47863610 MS.gene66702:CDS 40.0%
ACAAAGCTGCATCTCCAATT+AGG + chr4.3:47863874-47863893 MS.gene66702:CDS 40.0%
AGCTTCAAGACCATACTTCT+TGG - chr4.3:47862562-47862581 None:intergenic 40.0%
ATCAAAAGGTGATCAAGCCA+AGG + chr4.3:47863572-47863591 MS.gene66702:CDS 40.0%
ATGTCTGTATATCTGCAGGT+AGG + chr4.3:47863792-47863811 MS.gene66702:intron 40.0%
GATCAATGACAGAGTAATGG+AGG - chr4.3:47863634-47863653 None:intergenic 40.0%
GGAGAGGATGAATAATGACA+TGG + chr4.3:47863362-47863381 MS.gene66702:intron 40.0%
GGGAGCAAAATATGTGTTGT+GGG + chr4.3:47863089-47863108 MS.gene66702:intron 40.0%
GGGGAACAACAGAAATCAAA+AGG + chr4.3:47863558-47863577 MS.gene66702:CDS 40.0%
GGTTGAGTCGACAAAAATCA+AGG - chr4.3:47863322-47863341 None:intergenic 40.0%
TTCAGGTGAAGATTGCTTTG+AGG - chr4.3:47862532-47862551 None:intergenic 40.0%
! AAAGAAGCACAAGAAGGTGA+TGG - chr4.3:47862390-47862409 None:intergenic 40.0%
! AAGAAGTATGGTCTTGAAGC+TGG + chr4.3:47862561-47862580 MS.gene66702:CDS 40.0%
! ATATTTTGCCAGCAACTCCT+TGG - chr4.3:47863592-47863611 None:intergenic 40.0%
! CTCAGAAAAGAAGCACAAGA+AGG - chr4.3:47862396-47862415 None:intergenic 40.0%
! GAAGTGTCTCGTATAGTCTA+CGG - chr4.3:47863453-47863472 None:intergenic 40.0%
!! GGGTTATCGTTTTAATGGAG+AGG + chr4.3:47863346-47863365 MS.gene66702:intron 40.0%
!! TTATGGGAGTGTCATCATGT+CGG + chr4.3:47863394-47863413 MS.gene66702:intron 40.0%
!!! AAAGGTACCGACTTTTTCAC+CGG - chr4.3:47863835-47863854 None:intergenic 40.0%
!!! TTTTGTCGACTCAACCAGTT+GGG + chr4.3:47863326-47863345 MS.gene66702:intron 40.0%
!!! TTTTTGTCGACTCAACCAGT+TGG + chr4.3:47863325-47863344 MS.gene66702:intron 40.0%
AATCCAACCAGCTACGATTG+GGG - chr4.3:47863922-47863941 None:intergenic 45.0%
ACTGTGGGTGGAAACCTAAT+TGG - chr4.3:47863891-47863910 None:intergenic 45.0%
ATTGGGGTAAGAGCTACTGT+GGG - chr4.3:47863906-47863925 None:intergenic 45.0%
CAATCCAACCAGCTACGATT+GGG - chr4.3:47863923-47863942 None:intergenic 45.0%
GAGTTGCTGGCAAAATATGG+AGG + chr4.3:47863594-47863613 MS.gene66702:CDS 45.0%
GATGCTACCGGTGAAAAAGT+CGG + chr4.3:47863825-47863844 MS.gene66702:CDS 45.0%
GGAATCACTACTGATGCTAC+CGG + chr4.3:47863813-47863832 MS.gene66702:CDS 45.0%
GGGGAGCAAAATATGTGTTG+TGG + chr4.3:47863088-47863107 MS.gene66702:intron 45.0%
TGCAGCATATGCCAAAGCAA+AGG - chr4.3:47863853-47863872 None:intergenic 45.0%
TTGCCGAAATTTGCAGGGAA+AGG + chr4.3:47862844-47862863 MS.gene66702:intron 45.0%
! AAAAGCGCGGCTTTGACTTT+AGG - chr4.3:47863481-47863500 None:intergenic 45.0%
! AAAGCGCGGCTTTGACTTTA+GGG - chr4.3:47863480-47863499 None:intergenic 45.0%
! CAATGACAGAGTAATGGAGG+TGG - chr4.3:47863631-47863650 None:intergenic 45.0%
!! ATGGTCTTGAAGCTGGTCTT+TGG + chr4.3:47862568-47862587 MS.gene66702:CDS 45.0%
!! TCTTGAAGCTGGTCTTTGGA+AGG + chr4.3:47862572-47862591 MS.gene66702:CDS 45.0%
CCAATCCAACCAGCTACGAT+TGG - chr4.3:47863924-47863943 None:intergenic 50.0%
CCAATCGTAGCTGGTTGGAT+TGG + chr4.3:47863921-47863940 MS.gene66702:CDS 50.0%
GATTGGGGTAAGAGCTACTG+TGG - chr4.3:47863907-47863926 None:intergenic 50.0%
TGATCAAGCCAAGGAGTTGC+TGG + chr4.3:47863581-47863600 MS.gene66702:CDS 50.0%
TTACCCCAATCGTAGCTGGT+TGG + chr4.3:47863916-47863935 MS.gene66702:CDS 50.0%
!! GCGGCTTTGACTTTAGGGTT+AGG - chr4.3:47863475-47863494 None:intergenic 50.0%
GCTCTTACCCCAATCGTAGC+TGG + chr4.3:47863912-47863931 MS.gene66702:CDS 55.0%
GGGGTAAGAGCTACTGTGGG+TGG - chr4.3:47863903-47863922 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 47862366 47863968 47862366 ID=MS.gene66702
chr4.3 mRNA 47862366 47863968 47862366 ID=MS.gene66702.t1;Parent=MS.gene66702
chr4.3 exon 47862366 47862593 47862366 ID=MS.gene66702.t1.exon1;Parent=MS.gene66702.t1
chr4.3 CDS 47862366 47862593 47862366 ID=cds.MS.gene66702.t1;Parent=MS.gene66702.t1
chr4.3 exon 47863537 47863716 47863537 ID=MS.gene66702.t1.exon2;Parent=MS.gene66702.t1
chr4.3 CDS 47863537 47863716 47863537 ID=cds.MS.gene66702.t1;Parent=MS.gene66702.t1
chr4.3 exon 47863810 47863968 47863810 ID=MS.gene66702.t1.exon3;Parent=MS.gene66702.t1
chr4.3 CDS 47863810 47863968 47863810 ID=cds.MS.gene66702.t1;Parent=MS.gene66702.t1
Gene Sequence

>MS.gene66702

ATGACAACGTTTTCGTTGCTACCATCACCTTCTTGTGCTTCTTTTCTGAGCAACAAGAAGAGTTCAAGATTTTGCAGTCTTGCTTCCATTCAGTCACGTAAATCTAATGTTAAAGTGTTCAGAGTTAGAGCTGTTAAAGAGAAAACTGAGGAAATCAAAAGCTCCTCAAAGCAATCTTCACCTGAAGAAGTAACCAAGAAGTATGGTCTTGAAGCTGGTCTTTGGAAGGTGCCCATTTCTTTCTCTTATTTATTTTGGTTTAATTATGATTATGTGGTTAGTATAAAGTTTCAATTTCATTAACCTAAGTGAGAAATTCATCTTTTTGGGTTGCTTCTTGTTTATGTATCATTTGCTAGCATGCAACCTTTTTTTTTTCCTTCATTTTTATTGCTTTAAATGCAAAGAGTGTATTGTTTATACTCTATAAATCTGTTCTAAAAAGTATATATAAAGGATATTCAAAGATAGGATTTTATTGCCGAAATTTGCAGGGAAAGGAAATAAAGCTTTTAAAGAAAGAATTTAGTTTGAACTTTGAACAAGTTAGACAAAATGAGTAGTTTTCTTGTCATGTAGAACTTGGGCTGTATCAGATTAAATGTTGCGTTTAAAACAAGAGATATCACTTTATCAGTTATTTCGTGTGTTTGTTTACGAGTTTTATTTTAGGTTTTATTTCCATTTCTTGGTTTAGCATACTGTATCTTTCCCTGATTTTAGGGGAGCAAAATATGTGTTGTGGGTGAAATTTTACCTTCAAGAAATGTTCTGTACTGAATCAAGATTGAGTACCAATGTTTGCATATCGATTCTGACTACTTGTGTCGCCAATTTAAAGTAGAAGTTTGAATTTCCGACAATTTTACTGCTGAGATAGTAATTTAATCAGTGTTGATTTGTTAAAAATTATCAGAAATATAAGTTGAGTTTTAAGGTAGTGAAATTTGTTTCCTTGATTTTTGTCGACTCAACCAGTTGGGTTATCGTTTTAATGGAGAGGATGAATAATGACATGGATTTTATACTTATGGGAGTGTCATCATGTCGGTAGAGCTCCAAACGATTTTCAATCATTAGTTTACCGTAGACTATACGAGACACTTCCTAACCCTAAAGTCAAAGCCGCGCTTTTCATTCTGTGTAAAATGAAAATTATGGTCTTTTGCAGATTTTCAGTTCAAAGGAAGAAGGGGAACAACAGAAATCAAAAGGTGATCAAGCCAAGGAGTTGCTGGCAAAATATGGAGGAGCATACTTAGCCACCTCCATTACTCTGTCATTGATCTCTTTCGCTCTTTGTTATGTATTAATCAATGCTGGAGTAGATGTTCAAACTTTGCTTCAGAAGGTGCAGTGCTCATACATAACCCAATGTAGATTTAAGTATAATGAAAACAAAGTAACTTCTATGAATTAACACATTATGTCTGTATATCTGCAGGTAGGAATCACTACTGATGCTACCGGTGAAAAAGTCGGTACCTTTGCTTTGGCATATGCTGCACACAAAGCTGCATCTCCAATTAGGTTTCCACCCACAGTAGCTCTTACCCCAATCGTAGCTGGTTGGATTGGAAAGAAAGCTGATAAAGATAAGTAG

Protein sequence

>MS.gene66702.t1

MTTFSLLPSPSCASFLSNKKSSRFCSLASIQSRKSNVKVFRVRAVKEKTEEIKSSSKQSSPEEVTKKYGLEAGLWKIFSSKEEGEQQKSKGDQAKELLAKYGGAYLATSITLSLISFALCYVLINAGVDVQTLLQKVGITTDATGEKVGTFALAYAAHKAASPIRFPPTVALTPIVAGWIGKKADKDK