AlfalfaGEDB | MS.gene98295

M. sativa cultivar XinJiangDaYe / MS.gene98295

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98295.t1	AES72346.1	95.5	44	2	0	1	44	1	44	2.50E-14	88.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98295.t1	G7J5J1	95.5	44	2	0	1	44	2	45	1.8e-14	88.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04998	MS.gene98295	0.860876	1.52E-63	-1.69E-46
MS.gene051226	MS.gene98295	0.876329	1.52E-68	-1.69E-46
MS.gene051837	MS.gene98295	0.803574	3.27E-49	-1.69E-46
MS.gene054893	MS.gene98295	0.800662	1.30E-48	-1.69E-46
MS.gene057093	MS.gene98295	0.846851	1.68E-59	-1.69E-46
MS.gene057622	MS.gene98295	0.844787	6.09E-59	-1.69E-46
MS.gene060964	MS.gene98295	0.812884	3.39E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene98295	MS.gene64316	PPI
MS.gene015470	MS.gene98295	PPI
MS.gene015658	MS.gene98295	PPI
MS.gene22553	MS.gene98295	PPI
MS.gene53662	MS.gene98295	PPI
MS.gene98295	MS.gene64317	PPI
MS.gene80011	MS.gene98295	PPI
MS.gene96914	MS.gene98295	PPI
MS.gene070168	MS.gene98295	PPI
MS.gene98295	MS.gene64310	PPI
MS.gene026940	MS.gene98295	PPI
MS.gene21155	MS.gene98295	PPI
MS.gene92775	MS.gene98295	PPI
MS.gene004899	MS.gene98295	PPI
MS.gene80013	MS.gene98295	PPI
MS.gene98295	MS.gene79028	PPI
MS.gene09934	MS.gene98295	PPI
MS.gene96922	MS.gene98295	PPI
MS.gene009021	MS.gene98295	PPI
MS.gene46237	MS.gene98295	PPI
MS.gene070167	MS.gene98295	PPI
MS.gene98295	MS.gene21696	PPI
MS.gene87673	MS.gene98295	PPI
MS.gene05284	MS.gene98295	PPI
MS.gene21931	MS.gene98295	PPI
MS.gene041795	MS.gene98295	PPI
MS.gene046988	MS.gene98295	PPI
MS.gene63000	MS.gene98295	PPI
MS.gene98295	MS.gene025797	PPI
MS.gene057293	MS.gene98295	PPI
MS.gene98295	MS.gene64314	PPI
MS.gene63281	MS.gene98295	PPI
MS.gene53648	MS.gene98295	PPI
MS.gene68345	MS.gene98295	PPI
MS.gene017446	MS.gene98295	PPI
MS.gene89216	MS.gene98295	PPI
MS.gene040127	MS.gene98295	PPI
MS.gene62258	MS.gene98295	PPI
MS.gene64310	MS.gene98295	PPI
MS.gene53659	MS.gene98295	PPI
MS.gene038456	MS.gene98295	PPI
MS.gene000724	MS.gene98295	PPI
MS.gene98295	MS.gene53650	PPI
MS.gene052672	MS.gene98295	PPI
MS.gene64311	MS.gene98295	PPI
MS.gene25186	MS.gene98295	PPI
MS.gene008831	MS.gene98295	PPI
MS.gene96913	MS.gene98295	PPI
MS.gene58906	MS.gene98295	PPI
MS.gene020515	MS.gene98295	PPI
MS.gene58860	MS.gene98295	PPI
MS.gene27516	MS.gene98295	PPI
MS.gene02545	MS.gene98295	PPI
MS.gene053319	MS.gene98295	PPI
MS.gene04915	MS.gene98295	PPI
MS.gene61009	MS.gene98295	PPI
MS.gene64327	MS.gene98295	PPI
MS.gene64307	MS.gene98295	PPI
MS.gene98295	MS.gene21931	PPI
MS.gene026618	MS.gene98295	PPI
MS.gene04917	MS.gene98295	PPI
MS.gene64316	MS.gene98295	PPI
MS.gene98295	MS.gene53659	PPI
MS.gene98295	MS.gene09960	PPI
MS.gene074045	MS.gene98295	PPI
MS.gene47195	MS.gene98295	PPI
MS.gene066863	MS.gene98295	PPI
MS.gene036930	MS.gene98295	PPI
MS.gene98295	MS.gene061768	PPI
MS.gene98295	MS.gene89216	PPI
MS.gene001246	MS.gene98295	PPI
MS.gene013748	MS.gene98295	PPI
MS.gene55545	MS.gene98295	PPI
MS.gene98295	MS.gene93763	PPI
MS.gene98295	MS.gene80012	PPI
MS.gene066862	MS.gene98295	PPI
MS.gene024717	MS.gene98295	PPI
MS.gene64308	MS.gene98295	PPI
MS.gene64313	MS.gene98295	PPI
MS.gene83068	MS.gene98295	PPI
MS.gene93087	MS.gene98295	PPI
MS.gene62689	MS.gene98295	PPI
MS.gene97948	MS.gene98295	PPI
MS.gene003880	MS.gene98295	PPI
MS.gene54637	MS.gene98295	PPI
MS.gene000738	MS.gene98295	PPI
MS.gene67281	MS.gene98295	PPI
MS.gene061261	MS.gene98295	PPI
MS.gene025777	MS.gene98295	PPI
MS.gene98295	MS.gene056461	PPI
MS.gene062239	MS.gene98295	PPI
MS.gene46886	MS.gene98295	PPI
MS.gene98295	MS.gene98972	PPI
MS.gene026530	MS.gene98295	PPI
MS.gene98295	MS.gene29459	PPI
MS.gene71207	MS.gene98295	PPI
MS.gene07422	MS.gene98295	PPI
MS.gene03228	MS.gene98295	PPI
MS.gene21932	MS.gene98295	PPI
MS.gene28338	MS.gene98295	PPI
MS.gene85596	MS.gene98295	PPI
MS.gene94551	MS.gene98295	PPI
MS.gene65954	MS.gene98295	PPI
MS.gene008225	MS.gene98295	PPI
MS.gene056383	MS.gene98295	PPI
MS.gene64324	MS.gene98295	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98295.t1	MTR_3g089090	95.683	139	6	0	1	139	1	139	5.12e-93	265
MS.gene98295.t1	MTR_3g089070	95.683	139	6	0	1	139	2	140	5.72e-93	265
MS.gene98295.t1	MTR_4g103630	72.993	137	37	0	3	139	4	140	2.92e-68	202
MS.gene98295.t1	MTR_4g103650	74.436	133	34	0	6	138	3	135	4.95e-67	199
MS.gene98295.t1	MTR_4g103655	72.794	136	37	0	3	138	4	139	8.10e-67	199
MS.gene98295.t1	MTR_6g023460	54.745	137	62	0	2	138	5	141	4.72e-51	159
MS.gene98295.t1	MTR_8g078270	48.148	135	69	1	5	138	53	187	5.98e-35	120
MS.gene98295.t1	MTR_3g067610	38.931	131	77	1	9	139	9	136	2.57e-28	101
MS.gene98295.t1	MTR_5g079340	40.152	132	75	2	9	138	8	137	1.51e-27	99.8
MS.gene98295.t1	MTR_7g075040	40.972	144	75	4	6	139	48	191	2.36e-27	100
MS.gene98295.t1	MTR_8g107110	37.313	134	84	0	6	139	25	158	2.78e-27	99.8
MS.gene98295.t1	MTR_1g032070	39.552	134	80	1	6	138	47	180	3.66e-27	100
MS.gene98295.t1	MTR_1g046950	39.098	133	78	1	9	138	8	140	4.75e-26	95.9
MS.gene98295.t1	MTR_8g105230	39.231	130	78	1	6	134	37	166	3.13e-25	95.1
MS.gene98295.t1	MTR_8g036075	38.462	143	79	3	6	139	50	192	8.17e-25	94.4
MS.gene98295.t1	MTR_1g047100	36.765	136	76	2	9	138	8	139	1.21e-23	89.7
MS.gene98295.t1	MTR_6g079570	36.567	134	84	1	6	138	27	160	2.02e-22	87.4
MS.gene98295.t1	MTR_5g017510	36.090	133	84	1	7	138	50	182	2.30e-22	87.8
MS.gene98295.t1	MTR_5g017560	36.090	133	84	1	7	138	50	182	2.30e-22	87.8
MS.gene98295.t1	MTR_4g067270	36.429	140	80	3	6	139	6	142	2.94e-22	86.3
MS.gene98295.t1	MTR_4g086260	34.615	130	84	1	9	138	61	189	9.92e-21	83.6
MS.gene98295.t1	MTR_5g017550	35.821	134	82	2	7	138	50	181	3.08e-20	82.0
MS.gene98295.t1	MTR_1g076650	33.813	139	81	2	6	138	13	146	5.89e-20	80.5
MS.gene98295.t1	MTR_7g087610	31.655	139	84	2	6	138	13	146	9.23e-20	80.1
MS.gene98295.t1	MTR_6g025320	30.935	139	85	2	6	138	13	146	1.59e-18	76.6
MS.gene98295.t1	MTR_5g088320	30.935	139	85	2	6	138	13	146	1.64e-18	76.6
MS.gene98295.t1	MTR_1g041285	33.858	127	83	1	13	138	20	146	2.50e-18	76.3
MS.gene98295.t1	MTR_7g089760	34.074	135	86	2	8	139	8	142	5.49e-18	75.5
MS.gene98295.t1	MTR_8g066630	32.593	135	79	2	9	135	21	151	2.85e-17	73.9
MS.gene98295.t1	MTR_1g071150	30.370	135	94	0	4	138	11	145	7.69e-17	72.4
MS.gene98295.t1	MTR_5g008705	32.283	127	85	1	13	138	2	128	1.13e-16	71.6
MS.gene98295.t1	MTR_7g090450	32.847	137	86	3	8	138	76	212	3.40e-16	72.0
MS.gene98295.t1	MTR_5g008695	35.135	111	69	1	30	137	2	112	5.11e-16	69.3
MS.gene98295.t1	MTR_3g083470	31.579	133	80	2	7	138	95	217	5.89e-16	71.6
MS.gene98295.t1	MTR_4g112460	32.331	133	87	2	9	138	15	147	1.07e-15	70.1
MS.gene98295.t1	MTR_7g074240	32.143	140	82	2	13	139	32	171	2.62e-14	66.6
MS.gene98295.t1	MTR_2g098890	33.333	138	87	2	7	139	66	203	4.55e-14	66.6
MS.gene98295.t1	MTR_5g009830	32.117	137	88	2	5	139	371	504	6.45e-14	67.8
MS.gene98295.t1	MTR_8g099095	31.387	137	89	2	5	139	371	504	1.03e-13	67.0
MS.gene98295.t1	MTR_4g115170	31.169	154	83	5	7	139	66	217	2.26e-13	64.7
MS.gene98295.t1	MTR_8g069915	29.323	133	91	2	9	138	16	148	3.07e-13	63.5
MS.gene98295.t1	MTR_0028s0170	28.148	135	96	1	5	139	383	516	4.11e-13	65.5
MS.gene98295.t1	MTR_0168s0120	33.019	106	58	3	41	138	10	110	5.49e-13	61.6
MS.gene98295.t1	MTR_6g025320	33.019	106	58	3	41	138	10	110	5.49e-13	61.6
MS.gene98295.t1	MTR_7g115040	31.343	134	91	1	6	138	13	146	6.50e-13	62.4
MS.gene98295.t1	MTR_5g027000	28.030	132	94	1	9	139	26	157	9.92e-13	62.0
MS.gene98295.t1	MTR_5g089320	28.676	136	92	2	5	138	373	505	9.97e-13	64.3
MS.gene98295.t1	MTR_7g068710	31.746	126	86	0	14	139	370	495	2.46e-12	63.2
MS.gene98295.t1	MTR_6g007613	30.709	127	87	1	13	138	37	163	5.00e-12	60.5
MS.gene98295.t1	MTR_5g092810	29.927	137	91	2	5	139	397	530	5.18e-12	62.4
MS.gene98295.t1	MTR_3g098090	30.147	136	91	2	5	139	337	469	1.04e-11	61.2
MS.gene98295.t1	MTR_3g098070	30.147	136	91	2	5	139	334	466	1.11e-11	61.2
MS.gene98295.t1	MTR_7g091890	30.714	140	89	2	4	139	362	497	1.47e-11	60.8
MS.gene98295.t1	MTR_1g026190	27.007	137	95	2	5	139	387	520	1.51e-11	60.8
MS.gene98295.t1	MTR_1g052530	29.412	136	96	0	4	139	356	491	1.55e-11	60.8
MS.gene98295.t1	MTR_1g054865	30.147	136	93	1	4	139	465	598	2.21e-11	60.5
MS.gene98295.t1	MTR_7g074020	29.921	127	88	1	13	138	38	164	2.22e-11	58.5
MS.gene98295.t1	MTR_3g051770	28.369	141	95	2	5	139	388	528	5.85e-11	59.3
MS.gene98295.t1	MTR_4g132070	28.467	137	93	2	5	139	396	529	7.74e-11	58.9
MS.gene98295.t1	MTR_4g066660	30.435	138	89	3	5	139	377	510	8.62e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98295.t1	AT1G76650	45.652	138	75	0	2	139	40	177	1.37e-36	124
MS.gene98295.t1	AT1G76650	45.652	138	75	0	2	139	40	177	1.37e-36	124
MS.gene98295.t1	AT1G76640	45.455	143	71	3	2	139	19	159	6.13e-34	116
MS.gene98295.t1	AT3G50770	46.715	137	67	2	8	138	67	203	8.49e-34	117
MS.gene98295.t1	AT1G76650	44.203	138	67	1	2	139	40	167	1.89e-33	115
MS.gene98295.t1	AT5G42380	42.857	133	74	1	9	139	53	185	8.15e-32	112
MS.gene98295.t1	AT1G24620	40.299	134	79	1	6	138	38	171	2.68e-27	100
MS.gene98295.t1	AT3G01830	39.416	137	78	2	6	139	12	146	4.70e-26	95.9
MS.gene98295.t1	AT5G37770	35.971	139	84	1	6	139	18	156	4.72e-25	94.0
MS.gene98295.t1	AT5G37770	35.971	139	84	1	6	139	18	156	4.72e-25	94.0
MS.gene98295.t1	AT1G66400	33.824	136	87	1	6	138	16	151	6.02e-25	93.6
MS.gene98295.t1	AT1G18210	36.842	133	81	1	6	138	24	153	6.27e-24	91.3
MS.gene98295.t1	AT1G18210	36.842	133	81	1	6	138	24	153	6.27e-24	91.3
MS.gene98295.t1	AT1G73630	33.588	131	84	1	9	139	24	151	2.47e-22	87.0
MS.gene98295.t1	AT3G07490	35.915	142	88	2	1	139	1	142	7.38e-21	82.8
MS.gene98295.t1	AT1G05990	36.232	138	84	2	6	139	6	143	3.64e-20	81.3
MS.gene98295.t1	AT2G15680	34.328	134	85	2	7	139	52	183	7.82e-20	81.3
MS.gene98295.t1	AT3G59440	34.815	135	86	1	7	139	53	187	1.24e-19	80.9
MS.gene98295.t1	AT4G03290	34.483	145	89	2	1	139	1	145	2.60e-19	79.0
MS.gene98295.t1	AT3G22930	32.836	134	89	1	6	138	36	169	3.67e-19	79.0
MS.gene98295.t1	AT2G43290	33.099	142	87	2	6	139	66	207	4.11e-19	80.1
MS.gene98295.t1	AT5G17470	32.090	134	88	1	9	139	6	139	4.71e-19	78.2
MS.gene98295.t1	AT3G56800	31.655	139	84	2	6	138	13	146	7.53e-19	77.8
MS.gene98295.t1	AT2G27030	31.655	139	84	2	6	138	13	146	7.53e-19	77.8
MS.gene98295.t1	AT2G41110	31.655	139	84	2	6	138	13	146	7.53e-19	77.8
MS.gene98295.t1	AT5G21274	31.655	139	84	2	6	138	13	146	7.94e-19	77.8
MS.gene98295.t1	AT5G37780	30.935	139	85	2	6	138	13	146	9.23e-19	77.4
MS.gene98295.t1	AT1G66410	30.935	139	85	2	6	138	13	146	9.23e-19	77.4
MS.gene98295.t1	AT3G43810	30.935	139	85	2	6	138	13	146	1.67e-18	76.6
MS.gene98295.t1	AT2G27030	31.655	139	84	2	6	138	13	146	1.88e-18	77.4
MS.gene98295.t1	AT4G14640	32.090	134	90	1	6	138	14	147	4.12e-18	75.9
MS.gene98295.t1	AT4G12860	34.815	135	85	2	8	139	8	142	4.17e-18	75.9
MS.gene98295.t1	AT3G10190	33.083	133	87	2	8	138	73	205	5.57e-18	76.6
MS.gene98295.t1	AT3G43810	30.935	139	85	2	6	138	13	146	9.00e-18	76.3
MS.gene98295.t1	AT3G50360	33.594	128	84	1	13	139	35	162	1.44e-16	72.4
MS.gene98295.t1	AT2G41100	28.477	151	90	2	6	138	67	217	2.02e-16	73.9
MS.gene98295.t1	AT2G41100	28.477	151	90	2	6	138	67	217	2.02e-16	73.9
MS.gene98295.t1	AT2G41100	28.477	151	90	2	6	138	67	217	2.02e-16	73.9
MS.gene98295.t1	AT2G41100	28.477	151	90	2	6	138	102	252	2.91e-16	73.9
MS.gene98295.t1	AT2G41100	26.000	150	94	2	6	138	13	162	4.62e-13	65.1
MS.gene98295.t1	AT2G41100	28.477	151	90	2	6	138	102	252	2.91e-16	73.9
MS.gene98295.t1	AT2G41100	26.000	150	94	2	6	138	13	162	4.62e-13	65.1
MS.gene98295.t1	AT2G41110	29.801	151	83	3	6	138	13	158	3.50e-16	71.2
MS.gene98295.t1	AT2G36180	29.630	135	91	2	9	139	4	138	4.35e-16	70.5
MS.gene98295.t1	AT3G03400	30.657	137	86	4	1	131	1	134	8.19e-16	69.3
MS.gene98295.t1	AT5G37780	27.922	154	85	3	6	138	13	161	1.20e-14	67.0
MS.gene98295.t1	AT1G18530	30.827	133	89	1	9	138	11	143	1.22e-14	67.0
MS.gene98295.t1	AT4G23650	33.577	137	86	2	5	139	383	516	2.61e-14	68.9
MS.gene98295.t1	AT5G12180	31.111	135	92	1	5	139	378	511	4.98e-14	68.2
MS.gene98295.t1	AT5G37780	29.365	126	78	2	19	138	13	133	9.00e-14	64.3
MS.gene98295.t1	AT4G14640	30.579	121	83	1	19	138	9	129	9.38e-14	63.9
MS.gene98295.t1	AT5G19360	30.370	135	93	1	5	139	373	506	9.50e-14	67.4
MS.gene98295.t1	AT1G66410	29.365	126	78	2	19	138	36	156	1.36e-13	64.3
MS.gene98295.t1	AT3G51850	33.333	126	84	0	14	139	368	493	1.75e-13	66.6
MS.gene98295.t1	AT5G37780	29.365	126	78	2	19	138	52	172	1.88e-13	64.3
MS.gene98295.t1	AT1G32250	29.927	137	81	2	9	134	20	152	2.44e-13	63.9
MS.gene98295.t1	AT1G66410	33.019	106	58	3	41	138	10	110	3.40e-13	62.0
MS.gene98295.t1	AT1G66410	33.019	106	58	3	41	138	10	110	3.40e-13	62.0
MS.gene98295.t1	AT3G43810	33.019	106	58	3	41	138	10	110	5.75e-13	61.6
MS.gene98295.t1	AT3G43810	33.019	106	58	3	41	138	10	110	5.75e-13	61.6
MS.gene98295.t1	AT1G76040	29.927	137	91	2	5	139	178	311	1.03e-12	63.9
MS.gene98295.t1	AT1G76040	29.927	137	91	2	5	139	178	311	1.03e-12	63.9
MS.gene98295.t1	AT1G76040	29.927	137	91	2	5	139	416	549	1.21e-12	64.3
MS.gene98295.t1	AT2G41090	27.068	133	95	1	6	138	13	143	1.95e-12	62.0
MS.gene98295.t1	AT4G37010	27.273	132	95	1	9	139	35	166	2.26e-12	61.2
MS.gene98295.t1	AT3G51920	27.612	134	96	1	6	138	13	146	2.27e-12	60.8
MS.gene98295.t1	AT4G37010	27.273	132	95	1	9	139	31	162	2.52e-12	61.2
MS.gene98295.t1	AT3G03000	29.851	134	92	1	4	135	19	152	1.09e-11	59.3
MS.gene98295.t1	AT3G10660	31.655	139	87	4	4	139	490	623	1.31e-11	61.2
MS.gene98295.t1	AT3G10660	31.655	139	87	4	4	139	490	623	1.31e-11	61.2
MS.gene98295.t1	AT5G12480	29.197	137	96	1	4	139	363	499	1.58e-11	60.8
MS.gene98295.t1	AT5G12480	29.197	137	96	1	4	139	269	405	1.71e-11	60.8
MS.gene98295.t1	AT1G50700	27.737	137	94	2	5	139	378	511	3.41e-11	60.1
MS.gene98295.t1	AT4G09570	29.412	136	92	2	5	139	330	462	3.59e-11	60.1
MS.gene98295.t1	AT4G04695	28.148	135	96	1	5	139	336	469	3.74e-11	59.7
MS.gene98295.t1	AT1G35670	29.412	136	92	2	5	139	331	463	3.80e-11	59.7
MS.gene98295.t1	AT1G74740	32.308	130	80	3	14	139	373	498	4.17e-11	59.7
MS.gene98295.t1	AT2G41410	28.889	135	92	3	8	138	73	207	4.86e-11	58.5
MS.gene98295.t1	AT3G20410	27.737	137	94	2	5	139	396	529	7.02e-11	58.9
MS.gene98295.t1	AT3G20410	27.737	137	94	2	5	139	396	529	7.02e-11	58.9
MS.gene98295.t1	AT4G21940	29.197	137	92	2	5	139	406	539	7.85e-11	58.9
MS.gene98295.t1	AT4G21940	29.197	137	92	2	5	139	406	539	8.55e-11	58.9
MS.gene98295.t1	AT2G31500	31.655	139	92	2	4	139	370	508	9.77e-11	58.5

Find 27 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCATCCTCTTCAAATTCTT+TGG	0.258063	3.1:+61370877	None:intergenic
GGAGGAATTAATTGCTTTGA+TGG	0.320451	3.1:-61370983	MS.gene98295:CDS
TTTGTTGAAAGAAGCTGAAA+TGG	0.376240	3.1:-61371049	MS.gene98295:CDS
TGGTGATGGATATTTGAGTT+TGG	0.381496	3.1:-61371004	MS.gene98295:CDS
AGACAAAGGCTGGGAGTAAT+GGG	0.401953	3.1:-61371081	MS.gene98295:CDS
AGCCTTTGTCTTAGCTCTGC+TGG	0.405548	3.1:+61371093	None:intergenic
GAAGAGGATGCTTAAGAAGA+TGG	0.422617	3.1:-61370866	MS.gene98295:CDS
AGCAGAGCTAAGACAAAGGC+TGG	0.426380	3.1:-61371091	MS.gene98295:CDS
TTAATTGCTTTGATGGAGGA+AGG	0.458662	3.1:-61370976	MS.gene98295:CDS
AAGACAAAGGCTGGGAGTAA+TGG	0.460108	3.1:-61371082	MS.gene98295:CDS
AGAAGCTGAAATGGCTATTG+AGG	0.481748	3.1:-61371040	MS.gene98295:CDS
AAGAGGATGCTTAAGAAGAT+GGG	0.484014	3.1:-61370865	MS.gene98295:CDS
TGGAGAGGAACAAAAGTTGA+AGG	0.498848	3.1:-61370953	MS.gene98295:CDS
ATTGCTTTGATGGAGGAAGG+TGG	0.541567	3.1:-61370973	MS.gene98295:CDS
TAACACCAAAGAATTTGAAG+AGG	0.563578	3.1:-61370882	MS.gene98295:CDS
TGATGGATATTTGAGTTTGG+AGG	0.566788	3.1:-61371001	MS.gene98295:CDS
GGAATTAATTGCTTTGATGG+AGG	0.567051	3.1:-61370980	MS.gene98295:CDS
TGAAATGGCTATTGAGGCAA+TGG	0.572267	3.1:-61371034	MS.gene98295:CDS
TGATGAAGATGGTGATGGAA+AGG	0.579290	3.1:-61371121	MS.gene98295:CDS
CACCAGCAGAGCTAAGACAA+AGG	0.624826	3.1:-61371095	MS.gene98295:CDS
GCAGAGCTAAGACAAAGGCT+GGG	0.627677	3.1:-61371090	MS.gene98295:CDS
TTTGATGGAGGAAGGTGGAG+AGG	0.629126	3.1:-61370968	MS.gene98295:CDS
ATTGAGGCAATGGATTCAGA+TGG	0.638749	3.1:-61371024	MS.gene98295:CDS
ATGTATGATAGTGAGAAGTG+TGG	0.644233	3.1:-61370910	MS.gene98295:CDS
GCAATGGATTCAGATGGTGA+TGG	0.644671	3.1:-61371018	MS.gene98295:CDS
TTATAAAAGATGAAGAACGC+GGG	0.655567	3.1:-61371168	None:intergenic
ATTATAAAAGATGAAGAACG+CGG	0.665781	3.1:-61371169	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATGATTAAACATTTTGATT+TGG	-	chr3.1:61371109-61371128	MS.gene98295:CDS	15.0%
!!!	ATTAAACATTTTGATTTGGA+TGG	-	chr3.1:61371113-61371132	MS.gene98295:CDS	20.0%
!!!	TTAAACATTTTGATTTGGAT+GGG	-	chr3.1:61371114-61371133	MS.gene98295:CDS	20.0%
!!!	CTTAGATATTTTGATGAAGA+TGG	-	chr3.1:61370789-61370808	MS.gene98295:CDS	25.0%
!!!	TAAACATTTTGATTTGGATG+GGG	-	chr3.1:61371115-61371134	MS.gene98295:CDS	25.0%
	TAACACCAAAGAATTTGAAG+AGG	-	chr3.1:61371039-61371058	MS.gene98295:CDS	30.0%
	TTTGTTGAAAGAAGCTGAAA+TGG	-	chr3.1:61370872-61370891	MS.gene98295:CDS	30.0%
!!!	TATTTTGATGAAGATGGTGA+TGG	-	chr3.1:61370795-61370814	MS.gene98295:CDS	30.0%
	AAGAGGATGCTTAAGAAGAT+GGG	-	chr3.1:61371056-61371075	MS.gene98295:CDS	35.0%
	AGCATCCTCTTCAAATTCTT+TGG	+	chr3.1:61371047-61371066	None:intergenic	35.0%
	GGAATTAATTGCTTTGATGG+AGG	-	chr3.1:61370941-61370960	MS.gene98295:CDS	35.0%
	GGAGGAATTAATTGCTTTGA+TGG	-	chr3.1:61370938-61370957	MS.gene98295:CDS	35.0%
	TTAATTGCTTTGATGGAGGA+AGG	-	chr3.1:61370945-61370964	MS.gene98295:CDS	35.0%
!	ATGTATGATAGTGAGAAGTG+TGG	-	chr3.1:61371011-61371030	MS.gene98295:CDS	35.0%
!	TGATGGATATTTGAGTTTGG+AGG	-	chr3.1:61370920-61370939	MS.gene98295:CDS	35.0%
!!	TGGTGATGGATATTTGAGTT+TGG	-	chr3.1:61370917-61370936	MS.gene98295:CDS	35.0%
	AGAAGCTGAAATGGCTATTG+AGG	-	chr3.1:61370881-61370900	MS.gene98295:CDS	40.0%
	GAAGAGGATGCTTAAGAAGA+TGG	-	chr3.1:61371055-61371074	MS.gene98295:CDS	40.0%
	TGAAATGGCTATTGAGGCAA+TGG	-	chr3.1:61370887-61370906	MS.gene98295:CDS	40.0%
	TGGAGAGGAACAAAAGTTGA+AGG	-	chr3.1:61370968-61370987	MS.gene98295:CDS	40.0%
!	ATTGAGGCAATGGATTCAGA+TGG	-	chr3.1:61370897-61370916	MS.gene98295:CDS	40.0%
!!	TGATGAAGATGGTGATGGAA+AGG	-	chr3.1:61370800-61370819	MS.gene98295:CDS	40.0%
!!!	CATTTTGATTTGGATGGGGA+TGG	-	chr3.1:61371119-61371138	MS.gene98295:CDS	40.0%
	AAGACAAAGGCTGGGAGTAA+TGG	-	chr3.1:61370839-61370858	MS.gene98295:CDS	45.0%
	AGACAAAGGCTGGGAGTAAT+GGG	-	chr3.1:61370840-61370859	MS.gene98295:CDS	45.0%
	ATTGCTTTGATGGAGGAAGG+TGG	-	chr3.1:61370948-61370967	MS.gene98295:CDS	45.0%
!	GCAATGGATTCAGATGGTGA+TGG	-	chr3.1:61370903-61370922	MS.gene98295:CDS	45.0%
	AGCAGAGCTAAGACAAAGGC+TGG	-	chr3.1:61370830-61370849	MS.gene98295:CDS	50.0%
	CACCAGCAGAGCTAAGACAA+AGG	-	chr3.1:61370826-61370845	MS.gene98295:CDS	50.0%
	GCAGAGCTAAGACAAAGGCT+GGG	-	chr3.1:61370831-61370850	MS.gene98295:CDS	50.0%
	TTTGATGGAGGAAGGTGGAG+AGG	-	chr3.1:61370953-61370972	MS.gene98295:CDS	50.0%
!!	AGCCTTTGTCTTAGCTCTGC+TGG	+	chr3.1:61370831-61370850	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	61370762	61371181	61370762	ID=MS.gene98295
chr3.1	mRNA	61370762	61371181	61370762	ID=MS.gene98295.t1;Parent=MS.gene98295
chr3.1	exon	61370762	61371181	61370762	ID=MS.gene98295.t1.exon1;Parent=MS.gene98295.t1
chr3.1	CDS	61370762	61371181	61370762	ID=cds.MS.gene98295.t1;Parent=MS.gene98295.t1

Gene Sequence

>MS.gene98295

ATGAAGAACGCGGGATTCGAGCATGTTCTTAGATATTTTGATGAAGATGGTGATGGAAAGGTTTCACCAGCAGAGCTAAGACAAAGGCTGGGAGTAATGGGAGAAGAGATTTTGTTGAAAGAAGCTGAAATGGCTATTGAGGCAATGGATTCAGATGGTGATGGATATTTGAGTTTGGAGGAATTAATTGCTTTGATGGAGGAAGGTGGAGAGGAACAAAAGTTGAAGGATTTGAGAGATGCATTTGAGATGTATGATAGTGAGAAGTGTGGATTTATAACACCAAAGAATTTGAAGAGGATGCTTAAGAAGATGGGTGAGTCTAAGTCTATTGATGAATGTAAAGCTATGATTAAACATTTTGATTTGGATGGGGATGGTTTGCTTAGCTTTGATGAATTCACAGTAATGATGCAGTGA

Protein sequence

>MS.gene98295.t1

MKNAGFEHVLRYFDEDGDGKVSPAELRQRLGVMGEEILLKEAEMAIEAMDSDGDGYLSLEELIALMEEGGEEQKLKDLRDAFEMYDSEKCGFITPKNLKRMLKKMGESKSIDECKAMIKHFDLDGDGLLSFDEFTVMMQ