AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98949


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98949.t1 MTR_1g031740 83.333 144 17 2 1 138 1 143 1.24e-75 225
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAGGAGAATCTGGGTTT+TGG 0.221469 1.4:-16602414 None:intergenic
GGAGAGTGGCTGGGTGAAAA+TGG 0.232098 1.4:+16602865 MS.gene98949:CDS
GAAGAAGAAGGAGGAGAATC+TGG 0.270359 1.4:-16602421 None:intergenic
TGCTGAAGGCCCTGCGTGTT+GGG 0.285965 1.4:-16603300 None:intergenic
TGGAGGAAGATTGGTTTGTT+TGG 0.288556 1.4:-16602446 None:intergenic
GGTCGACGGGGAAGCTTGTT+TGG 0.309086 1.4:-16602520 None:intergenic
CTGCTGAAGGCCCTGCGTGT+TGG 0.314819 1.4:-16603301 None:intergenic
ATTAGTAATAAGAATGGATT+TGG 0.315469 1.4:-16602380 None:intergenic
TCTCGAGATCTACAAGTCTT+TGG 0.331857 1.4:+16603234 MS.gene98949:CDS
GGAGGAAGATTGGTTTGTTT+GGG 0.355312 1.4:-16602445 None:intergenic
TGCGTGTTGGGTGGTTTGCT+TGG 0.363451 1.4:-16603288 None:intergenic
GACCACGATGGATTTGTTCT+TGG 0.365556 1.4:+16602798 MS.gene98949:CDS
ACCACGATGGATTTGTTCTT+GGG 0.379690 1.4:+16602799 MS.gene98949:CDS
GAGAGTGGCTGGGTGAAAAT+GGG 0.391269 1.4:+16602866 MS.gene98949:CDS
GGTGTTAGTGGAGCGAAATC+TGG 0.391808 1.4:-16602893 None:intergenic
TTGCTACTTACTGGTGTTAG+TGG 0.404594 1.4:-16602905 None:intergenic
CGAAGATGGTGATGGTCGAC+GGG 0.447807 1.4:-16602533 None:intergenic
GAGGGAAACAGGAGAGTGGC+TGG 0.452230 1.4:+16602855 MS.gene98949:CDS
TCAATTTGCATGAGCGAAGA+TGG 0.459616 1.4:-16602547 None:intergenic
GCGTGTTGGGTGGTTTGCTT+GGG 0.461631 1.4:-16603287 None:intergenic
GTCTCATAGTGTTGAGGGAG+AGG 0.464051 1.4:-16603332 None:intergenic
AAGAAGAAGGAGGAGAATCT+GGG 0.467707 1.4:-16602420 None:intergenic
GTTTGTTTGGGTGAAGAAGA+AGG 0.476332 1.4:-16602433 None:intergenic
AGTTGCATTAGTAATAAGAA+TGG 0.478683 1.4:-16602386 None:intergenic
TTCTTGGGACAAGTATTTGA+AGG 0.485835 1.4:+16602814 MS.gene98949:CDS
AAGCAAACCACCCAACACGC+AGG 0.489944 1.4:+16603290 MS.gene98949:CDS
AGTGTTGAGGGAGAGGCAAT+AGG 0.490057 1.4:-16603325 None:intergenic
ATTGAACGTGCCCTTGGCGC+TGG 0.500974 1.4:+16602565 MS.gene98949:CDS
TAGATGTCTCATAGTGTTGA+GGG 0.510803 1.4:-16603337 None:intergenic
ATGCAAATTGAACGTGCCCT+TGG 0.511985 1.4:+16602559 MS.gene98949:CDS
GAGCATGCATACTTGAGGTC+AGG 0.520818 1.4:-16602607 None:intergenic
CTCTGAAGCTACCAGCGCCA+AGG 0.529782 1.4:-16602576 None:intergenic
AGCAAACCACCCAACACGCA+GGG 0.539603 1.4:+16603291 MS.gene98949:CDS
GCGAAGATGGTGATGGTCGA+CGG 0.545160 1.4:-16602534 None:intergenic
CTAGATGTCTCATAGTGTTG+AGG 0.553465 1.4:-16603338 None:intergenic
TCTTGGGACAAGTATTTGAA+GGG 0.567555 1.4:+16602815 MS.gene98949:CDS
TGAAGGCCCTGCGTGTTGGG+TGG 0.574001 1.4:-16603297 None:intergenic
CGACGGGGAAGCTTGTTTGG+AGG 0.576924 1.4:-16602517 None:intergenic
AGAGTGGCTGGGTGAAAATG+GGG 0.590303 1.4:+16602867 MS.gene98949:CDS
TCTGAAGCTACCAGCGCCAA+GGG 0.590855 1.4:-16602575 None:intergenic
TATCTAGTTCAAACACTACA+TGG 0.602133 1.4:+16603258 MS.gene98949:CDS
CTCGAGACAAGCAATGGACG+TGG 0.602857 1.4:-16603218 None:intergenic
TGTTTGGGTGAAGAAGAAGG+AGG 0.610626 1.4:-16602430 None:intergenic
AGGGAAACAGGAGAGTGGCT+GGG 0.620803 1.4:+16602856 MS.gene98949:CDS
GGCGCTGGTAGCTTCAGAGA+CGG 0.635842 1.4:+16602580 MS.gene98949:CDS
AGAGGCAATAGGTCTGCTGA+AGG 0.636549 1.4:-16603314 None:intergenic
GTAGATCTCGAGACAAGCAA+TGG 0.668961 1.4:-16603224 None:intergenic
AGCTGAGGGAAACAGGAGAG+TGG 0.671817 1.4:+16602851 MS.gene98949:CDS
GAAGATGGTGATGGTCGACG+GGG 0.719959 1.4:-16602532 None:intergenic
TGCATGAGCGAAGATGGTGA+TGG 0.732147 1.4:-16602541 None:intergenic
TCCCAAGAACAAATCCATCG+TGG 0.736351 1.4:-16602800 None:intergenic
AGACGTAAAGAAGACCACGA+TGG 0.745068 1.4:+16602786 MS.gene98949:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAACATTATGATGAAT+AGG + chr1.4:16602660-16602679 MS.gene98949:intron 15.0%
!!! TTGTTTTTTAAATAAGAAAC+TGG + chr1.4:16603097-16603116 MS.gene98949:intron 15.0%
!! GTTGTAAAAAGTAATAATCT+TGG + chr1.4:16603155-16603174 MS.gene98949:intron 20.0%
!! TGTTAAAGAGACAAAAAATA+AGG - chr1.4:16602946-16602965 None:intergenic 20.0%
!!! AGATTATTACTTTTTACAAC+TGG - chr1.4:16603155-16603174 None:intergenic 20.0%
!!! ATTAGTAATAAGAATGGATT+TGG - chr1.4:16602383-16602402 None:intergenic 20.0%
!!! CACTTTTAACATTATGAAAT+TGG - chr1.4:16602702-16602721 None:intergenic 20.0%
!!! TATTTTTTGTCTCTTTAACA+AGG + chr1.4:16602946-16602965 MS.gene98949:intron 20.0%
! AGTTGCATTAGTAATAAGAA+TGG - chr1.4:16602389-16602408 None:intergenic 25.0%
! ATCAAAATCCAGAATGAAAT+TGG - chr1.4:16602727-16602746 None:intergenic 25.0%
!! TAATGAAGTAGTAGAGTTTT+TGG - chr1.4:16602497-16602516 None:intergenic 25.0%
! ATAAGTGAAGGTTTTTGCTT+TGG - chr1.4:16602469-16602488 None:intergenic 30.0%
! TATCTAGTTCAAACACTACA+TGG + chr1.4:16603258-16603277 MS.gene98949:CDS 30.0%
!! CATGTCTTTTGCTTTGTTAT+AGG + chr1.4:16602980-16602999 MS.gene98949:intron 30.0%
!!! TTTTTGGAGCTTATAAGTGA+AGG - chr1.4:16602481-16602500 None:intergenic 30.0%
GTTATAGGTGATTATGAGTG+AGG + chr1.4:16602995-16603014 MS.gene98949:intron 35.0%
TCTTGGGACAAGTATTTGAA+GGG + chr1.4:16602815-16602834 MS.gene98949:CDS 35.0%
TTCTTGGGACAAGTATTTGA+AGG + chr1.4:16602814-16602833 MS.gene98949:CDS 35.0%
! AAAGTGCTCCAATTTCATTC+TGG + chr1.4:16602716-16602735 MS.gene98949:intron 35.0%
! TAGATGTCTCATAGTGTTGA+GGG - chr1.4:16603340-16603359 None:intergenic 35.0%
!!! TTTTTGCTTTGGAGGAAGAT+TGG - chr1.4:16602458-16602477 None:intergenic 35.0%
AAACAGAGCATGCATACTTG+AGG - chr1.4:16602615-16602634 None:intergenic 40.0%
AAGAAGAAGGAGGAGAATCT+GGG - chr1.4:16602423-16602442 None:intergenic 40.0%
CTAGATGTCTCATAGTGTTG+AGG - chr1.4:16603341-16603360 None:intergenic 40.0%
GTTTGTTTGGGTGAAGAAGA+AGG - chr1.4:16602436-16602455 None:intergenic 40.0%
TAACACCAGTAAGTAGCAAC+TGG + chr1.4:16602909-16602928 MS.gene98949:intron 40.0%
TCAATTTGCATGAGCGAAGA+TGG - chr1.4:16602550-16602569 None:intergenic 40.0%
TCTCGAGATCTACAAGTCTT+TGG + chr1.4:16603234-16603253 MS.gene98949:CDS 40.0%
! ACCACGATGGATTTGTTCTT+GGG + chr1.4:16602799-16602818 MS.gene98949:CDS 40.0%
! AGTGAAGGTTTTTGCTTTGG+AGG - chr1.4:16602466-16602485 None:intergenic 40.0%
! TTGCTACTTACTGGTGTTAG+TGG - chr1.4:16602908-16602927 None:intergenic 40.0%
!! GGAGGAAGATTGGTTTGTTT+GGG - chr1.4:16602448-16602467 None:intergenic 40.0%
!! TGGAGGAAGATTGGTTTGTT+TGG - chr1.4:16602449-16602468 None:intergenic 40.0%
AGACGTAAAGAAGACCACGA+TGG + chr1.4:16602786-16602805 MS.gene98949:CDS 45.0%
ATGCAAATTGAACGTGCCCT+TGG + chr1.4:16602559-16602578 MS.gene98949:CDS 45.0%
CAGAAAAAGCTGAGGGAAAC+AGG + chr1.4:16602844-16602863 MS.gene98949:CDS 45.0%
GAAGAAGAAGGAGGAGAATC+TGG - chr1.4:16602424-16602443 None:intergenic 45.0%
GACTGTGCAGAAAAAGCTGA+GGG + chr1.4:16602837-16602856 MS.gene98949:CDS 45.0%
GTAGATCTCGAGACAAGCAA+TGG - chr1.4:16603227-16603246 None:intergenic 45.0%
TCCCAAGAACAAATCCATCG+TGG - chr1.4:16602803-16602822 None:intergenic 45.0%
TGTTTGGGTGAAGAAGAAGG+AGG - chr1.4:16602433-16602452 None:intergenic 45.0%
! AAGGAGGAGAATCTGGGTTT+TGG - chr1.4:16602417-16602436 None:intergenic 45.0%
! AGGAGGAGAATCTGGGTTTT+GGG - chr1.4:16602416-16602435 None:intergenic 45.0%
! CGTTACCAGTTGCTACTTAC+TGG - chr1.4:16602917-16602936 None:intergenic 45.0%
! GACCACGATGGATTTGTTCT+TGG + chr1.4:16602798-16602817 MS.gene98949:CDS 45.0%
AGAGGCAATAGGTCTGCTGA+AGG - chr1.4:16603317-16603336 None:intergenic 50.0%
AGAGTGGCTGGGTGAAAATG+GGG + chr1.4:16602867-16602886 MS.gene98949:CDS 50.0%
CGAGGCCTTCGCTAAATTGT+AGG + chr1.4:16603195-16603214 MS.gene98949:intron 50.0%
CGTGGCCTACAATTTAGCGA+AGG - chr1.4:16603203-16603222 None:intergenic 50.0%
GAGAGTGGCTGGGTGAAAAT+GGG + chr1.4:16602866-16602885 MS.gene98949:CDS 50.0%
GAGCATGCATACTTGAGGTC+AGG - chr1.4:16602610-16602629 None:intergenic 50.0%
GGACTGTGCAGAAAAAGCTG+AGG + chr1.4:16602836-16602855 MS.gene98949:CDS 50.0%
GGTGTTAGTGGAGCGAAATC+TGG - chr1.4:16602896-16602915 None:intergenic 50.0%
TGCATGAGCGAAGATGGTGA+TGG - chr1.4:16602544-16602563 None:intergenic 50.0%
! AGTGTTGAGGGAGAGGCAAT+AGG - chr1.4:16603328-16603347 None:intergenic 50.0%
! GAGACTAGCTGATGAAGACG+AGG + chr1.4:16603177-16603196 MS.gene98949:intron 50.0%
! GGAGGAGAATCTGGGTTTTG+GGG - chr1.4:16602415-16602434 None:intergenic 50.0%
! GTCTCATAGTGTTGAGGGAG+AGG - chr1.4:16603335-16603354 None:intergenic 50.0%
AAGCAAACCACCCAACACGC+AGG + chr1.4:16603290-16603309 MS.gene98949:CDS 55.0%
AGCAAACCACCCAACACGCA+GGG + chr1.4:16603291-16603310 MS.gene98949:CDS 55.0%
AGCTGAGGGAAACAGGAGAG+TGG + chr1.4:16602851-16602870 MS.gene98949:CDS 55.0%
AGGGAAACAGGAGAGTGGCT+GGG + chr1.4:16602856-16602875 MS.gene98949:CDS 55.0%
CTCGAGACAAGCAATGGACG+TGG - chr1.4:16603221-16603240 None:intergenic 55.0%
GGAGAGTGGCTGGGTGAAAA+TGG + chr1.4:16602865-16602884 MS.gene98949:CDS 55.0%
! TCTGAAGCTACCAGCGCCAA+GGG - chr1.4:16602578-16602597 None:intergenic 55.0%
!! CGAAGATGGTGATGGTCGAC+GGG - chr1.4:16602536-16602555 None:intergenic 55.0%
!! GAAGATGGTGATGGTCGACG+GGG - chr1.4:16602535-16602554 None:intergenic 55.0%
!! GCGAAGATGGTGATGGTCGA+CGG - chr1.4:16602537-16602556 None:intergenic 55.0%
!! GCGTGTTGGGTGGTTTGCTT+GGG - chr1.4:16603290-16603309 None:intergenic 55.0%
!! TGCGTGTTGGGTGGTTTGCT+TGG - chr1.4:16603291-16603310 None:intergenic 55.0%
ATTGAACGTGCCCTTGGCGC+TGG + chr1.4:16602565-16602584 MS.gene98949:CDS 60.0%
GAGGGAAACAGGAGAGTGGC+TGG + chr1.4:16602855-16602874 MS.gene98949:CDS 60.0%
TGCTGAAGGCCCTGCGTGTT+GGG - chr1.4:16603303-16603322 None:intergenic 60.0%
! CGACGGGGAAGCTTGTTTGG+AGG - chr1.4:16602520-16602539 None:intergenic 60.0%
! GGTCGACGGGGAAGCTTGTT+TGG - chr1.4:16602523-16602542 None:intergenic 60.0%
!! CTCTGAAGCTACCAGCGCCA+AGG - chr1.4:16602579-16602598 None:intergenic 60.0%
!! GGCGCTGGTAGCTTCAGAGA+CGG + chr1.4:16602580-16602599 MS.gene98949:CDS 60.0%
CTGCTGAAGGCCCTGCGTGT+TGG - chr1.4:16603304-16603323 None:intergenic 65.0%
TGAAGGCCCTGCGTGTTGGG+TGG - chr1.4:16603300-16603319 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 16602349 16603376 16602349 ID=MS.gene98949
chr1.4 mRNA 16602349 16603376 16602349 ID=MS.gene98949.t1;Parent=MS.gene98949
chr1.4 exon 16602349 16602617 16602349 ID=MS.gene98949.t1.exon1;Parent=MS.gene98949.t1
chr1.4 CDS 16602349 16602617 16602349 ID=cds.MS.gene98949.t1;Parent=MS.gene98949.t1
chr1.4 exon 16602771 16602916 16602771 ID=MS.gene98949.t1.exon2;Parent=MS.gene98949.t1
chr1.4 CDS 16602771 16602916 16602771 ID=cds.MS.gene98949.t1;Parent=MS.gene98949.t1
chr1.4 exon 16603002 16603011 16603002 ID=MS.gene98949.t1.exon3;Parent=MS.gene98949.t1
chr1.4 CDS 16603002 16603011 16603002 ID=cds.MS.gene98949.t1;Parent=MS.gene98949.t1
chr1.4 exon 16603217 16603376 16603217 ID=MS.gene98949.t1.exon4;Parent=MS.gene98949.t1
chr1.4 CDS 16603217 16603376 16603217 ID=cds.MS.gene98949.t1;Parent=MS.gene98949.t1
Gene Sequence

>MS.gene98949

ATGTTTTGCTTATGCAACAATCTTTCTATAACCAAATCCATTCTTATTACTAATGCAACTCTTCCCCAAAACCCAGATTCTCCTCCTTCTTCTTCACCCAAACAAACCAATCTTCCTCCAAAGCAAAAACCTTCACTTATAAGCTCCAAAAACTCTACTACTTCATTACCTCCAAACAAGCTTCCCCGTCGACCATCACCATCTTCGCTCATGCAAATTGAACGTGCCCTTGGCGCTGGTAGCTTCAGAGACGGTGAACCTGACCTCAAGTATGCATGCTCTGTTTGAATAAACAACTTATTTTGCAGCTTATTATAACATTATGATGAATAGGCTATTTCTATTCACTACCAATTTCATAATGTTAAAAGTGCTCCAATTTCATTCTGGATTTTGATTTTTCTGCATTTTTTTTTTAAAAGAATGAAGAACGATTCAGACGTAAAGAAGACCACGATGGATTTGTTCTTGGGACAAGTATTTGAAGGGACTGTGCAGAAAAAGCTGAGGGAAACAGGAGAGTGGCTGGGTGAAAATGGGGAGACCAGATTTCGCTCCACTAACACCAGTAAGTAGCAACTGGTAACGACTTTTCCTTATTTTTTGTCTCTTTAACAAGGATTGTAGCATGCATGTCTTTTGCTTTGTTATAGGTGATTATGAGTGAGGAAAAACAGAAGTTATAGAGTAATAGTATGAATGATTAATTGAGCACATATCTTTATAGCATTTATTCTTGAGATAACATTTGTTTTTTAAATAAGAAACTGGATGCAATTTATTATCAATCAAAAATGAAATAACCAGTTGTAAAAAGTAATAATCTTGGAGACTAGCTGATGAAGACGAGGCCTTCGCTAAATTGTAGGCCACGTCCATTGCTTGTCTCGAGATCTACAAGTCTTTGGCTATCTAGTTCAAACACTACATGGTGCAAACCCAAGCAAACCACCCAACACGCAGGGCCTTCAGCAGACCTATTGCCTCTCCCTCAACACTATGAGACATCTAGTCAGTTCTTGAACTGA

Protein sequence

>MS.gene98949.t1

MFCLCNNLSITKSILITNATLPQNPDSPPSSSPKQTNLPPKQKPSLISSKNSTTSLPPNKLPRRPSPSSLMQIERALGAGSFRDGEPDLKMKNDSDVKKTTMDLFLGQVFEGTVQKKLRETGEWLGENGETRFRSTNTSDYEPRPLLVSRSTSLWLSSSNTTWCKPKQTTQHAGPSADLLPLPQHYETSSQFLN