AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043287.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043287.01.T01 MTR_8g432480 96.237 186 6 1 1 186 1 185 4.75e-129 360
MsG0880043287.01.T01 MTR_8g432440 54.494 178 57 6 7 167 5 175 2.04e-53 168
MsG0880043287.01.T01 MTR_8g432420 54.494 178 57 6 7 167 5 175 2.04e-53 168
MsG0880043287.01.T01 MTR_8g432460 54.237 177 60 5 7 168 5 175 4.10e-52 165
MsG0880043287.01.T01 MTR_8g432450 51.648 182 57 5 7 167 5 176 1.54e-50 161
MsG0880043287.01.T01 MTR_8g432470 51.977 177 63 5 7 167 5 175 3.41e-50 160
MsG0880043287.01.T01 MTR_8g432490 52.273 176 63 5 7 167 5 174 3.39e-49 157
MsG0880043287.01.T01 MTR_3g064780 51.977 177 64 5 7 168 5 175 1.72e-48 158
MsG0880043287.01.T01 MTR_8g432370 37.209 129 74 4 7 131 2 127 1.20e-21 87.4
MsG0880043287.01.T01 MTR_8g432400 38.281 128 75 3 7 131 2 128 2.93e-21 86.3
MsG0880043287.01.T01 MTR_8g432390 38.462 130 72 5 7 131 2 128 8.94e-21 85.1
MsG0880043287.01.T01 MTR_8g432350 36.923 130 74 5 7 131 2 128 6.36e-20 82.8
MsG0880043287.01.T01 MTR_7g076000 37.059 170 86 9 7 168 2 158 1.41e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCATTGGGATTCCAGTTT+TGG 0.209973 8:-22000189 MsG0880043287.01.T01:CDS
TTAGAAATCACCATTATTTC+CGG 0.251148 8:-22000647 MsG0880043287.01.T01:CDS
AAAATGAGGGTTTACTTTCA+TGG 0.303350 8:-22000526 MsG0880043287.01.T01:CDS
GAGTTATGGGTTGAGGGATT+GGG 0.319102 8:-22000342 MsG0880043287.01.T01:CDS
TGAGTTATGGGTTGAGGGAT+TGG 0.329761 8:-22000343 MsG0880043287.01.T01:CDS
AATTCAAATCATGTTGTCAT+TGG 0.330631 8:-22000203 MsG0880043287.01.T01:CDS
ATGTTCAAACTTTGAGTTAT+GGG 0.393724 8:-22000355 MsG0880043287.01.T01:CDS
AGTGGCTAGGATAGCCCTTT+CGG 0.395513 8:-22000411 MsG0880043287.01.T01:CDS
ATTCAAATCATGTTGTCATT+GGG 0.399832 8:-22000202 MsG0880043287.01.T01:CDS
AACTTTGAGTTATGGGTTGA+GGG 0.424777 8:-22000348 MsG0880043287.01.T01:CDS
GATGTTCAAACTTTGAGTTA+TGG 0.428751 8:-22000356 MsG0880043287.01.T01:CDS
TATTGAGGATGTGGGGTTGA+AGG 0.431275 8:-22000237 MsG0880043287.01.T01:CDS
AAACTTTGAGTTATGGGTTG+AGG 0.441165 8:-22000349 MsG0880043287.01.T01:CDS
AGAACAAGGTATTGAGGATG+TGG 0.474654 8:-22000246 MsG0880043287.01.T01:CDS
TGCAAAATCAGTGACGTTTG+AGG 0.499980 8:-22000471 MsG0880043287.01.T01:CDS
TGAGGATGTGGGGTTGAAGG+TGG 0.512663 8:-22000234 MsG0880043287.01.T01:CDS
TACAAAGGAGCTCGTCCCAT+TGG 0.515512 8:-22000434 MsG0880043287.01.T01:CDS
AAGTGTTGATCGAAATTCAA+AGG 0.521135 8:-22000615 MsG0880043287.01.T01:CDS
GCTATCCTAGCCACTCCAAT+GGG 0.521541 8:+22000419 None:intergenic
GAACAAGGTATTGAGGATGT+GGG 0.522715 8:-22000245 MsG0880043287.01.T01:CDS
GGTTGAGGGATTGGGATGGT+AGG 0.532351 8:-22000334 MsG0880043287.01.T01:CDS
GGCTATCCTAGCCACTCCAA+TGG 0.540754 8:+22000418 None:intergenic
AGTGTTGATCGAAATTCAAA+GGG 0.547290 8:-22000614 MsG0880043287.01.T01:CDS
GTCACCAAGAAAATCCGAAA+GGG 0.572767 8:+22000397 None:intergenic
GTGATTTCTAATGTGCGTGC+TGG 0.574354 8:+22000659 None:intergenic
GTGAGAAGGGTGACACAAGA+AGG 0.595191 8:-22000287 MsG0880043287.01.T01:CDS
TATGGGTTGAGGGATTGGGA+TGG 0.614237 8:-22000338 MsG0880043287.01.T01:CDS
AACAAGGTATTGAGGATGTG+GGG 0.625303 8:-22000244 MsG0880043287.01.T01:CDS
AAAATGGTGAAGGAAAATGA+GGG 0.646639 8:-22000539 MsG0880043287.01.T01:CDS
CTCGTCCCATTGGAGTGGCT+AGG 0.651174 8:-22000424 MsG0880043287.01.T01:CDS
GTACAATGCAAGAAGTACAA+AGG 0.653282 8:-22000449 MsG0880043287.01.T01:CDS
CGTCACCAAGAAAATCCGAA+AGG 0.654815 8:+22000396 None:intergenic
AGGAGCTCGTCCCATTGGAG+TGG 0.656408 8:-22000429 MsG0880043287.01.T01:CDS
TATTTATGTTGTTGTTCGTG+CGG 0.668109 8:-22000585 MsG0880043287.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAATTTTAGTGTGAGAA+GGG - Chr8:22000510-22000529 MsG0880043287.01.T01:CDS 20.0%
!!! TTATTAATTTTAGTGTGAGA+AGG - Chr8:22000509-22000528 MsG0880043287.01.T01:CDS 20.0%
! AATTCAAATCATGTTGTCAT+TGG - Chr8:22000607-22000626 MsG0880043287.01.T01:CDS 25.0%
! ATTCAAATCATGTTGTCATT+GGG - Chr8:22000608-22000627 MsG0880043287.01.T01:CDS 25.0%
! TTAGAAATCACCATTATTTC+CGG - Chr8:22000163-22000182 MsG0880043287.01.T01:CDS 25.0%
!! ATGTTCAAACTTTGAGTTAT+GGG - Chr8:22000455-22000474 MsG0880043287.01.T01:CDS 25.0%
!!! TTGGGTTTTTTGTTTCTTTT+GGG - Chr8:22000640-22000659 MsG0880043287.01.T01:CDS 25.0%
!!! TTTGGGTTTTTTGTTTCTTT+TGG - Chr8:22000639-22000658 MsG0880043287.01.T01:CDS 25.0%
AAAAATGGTGAAGGAAAATG+AGG - Chr8:22000270-22000289 MsG0880043287.01.T01:CDS 30.0%
AAAATGAGGGTTTACTTTCA+TGG - Chr8:22000284-22000303 MsG0880043287.01.T01:CDS 30.0%
AAAATGGTGAAGGAAAATGA+GGG - Chr8:22000271-22000290 MsG0880043287.01.T01:CDS 30.0%
AGAAACAAAAAACCCAAAAC+TGG + Chr8:22000637-22000656 None:intergenic 30.0%
TTGTGTGTAGAAAAAGAACA+AGG - Chr8:22000550-22000569 MsG0880043287.01.T01:CDS 30.0%
! ATCAACACTTATGTTTTGTC+CGG + Chr8:22000185-22000204 None:intergenic 30.0%
! GATGTTCAAACTTTGAGTTA+TGG - Chr8:22000454-22000473 MsG0880043287.01.T01:CDS 30.0%
! TATGTTTTGTCCGGAAATAA+TGG + Chr8:22000176-22000195 None:intergenic 30.0%
! TATTTATGTTGTTGTTCGTG+CGG - Chr8:22000225-22000244 MsG0880043287.01.T01:CDS 30.0%
!! AAGTGTTGATCGAAATTCAA+AGG - Chr8:22000195-22000214 MsG0880043287.01.T01:CDS 30.0%
!! AGAAAAAGAACAAGGTATTG+AGG - Chr8:22000558-22000577 MsG0880043287.01.T01:CDS 30.0%
!! AGTGTTGATCGAAATTCAAA+GGG - Chr8:22000196-22000215 MsG0880043287.01.T01:CDS 30.0%
! AAACTTTGAGTTATGGGTTG+AGG - Chr8:22000461-22000480 MsG0880043287.01.T01:CDS 35.0%
! AACTTTGAGTTATGGGTTGA+GGG - Chr8:22000462-22000481 MsG0880043287.01.T01:CDS 35.0%
! GTACAATGCAAGAAGTACAA+AGG - Chr8:22000361-22000380 MsG0880043287.01.T01:CDS 35.0%
CAACTCTTGCACGACAAAAA+TGG - Chr8:22000255-22000274 MsG0880043287.01.T01:CDS 40.0%
GATTGGGATGGTAGGAAAAA+TGG - Chr8:22000484-22000503 MsG0880043287.01.T01:CDS 40.0%
GTCACCAAGAAAATCCGAAA+GGG + Chr8:22000416-22000435 None:intergenic 40.0%
TGCAAAATCAGTGACGTTTG+AGG - Chr8:22000339-22000358 MsG0880043287.01.T01:CDS 40.0%
TTGCACGACAAAAATGGTGA+AGG - Chr8:22000261-22000280 MsG0880043287.01.T01:CDS 40.0%
! ATAGCCCTTTCGGATTTTCT+TGG - Chr8:22000409-22000428 MsG0880043287.01.T01:CDS 40.0%
! GTCATTGGGATTCCAGTTTT+GGG - Chr8:22000622-22000641 MsG0880043287.01.T01:CDS 40.0%
! TGTCATTGGGATTCCAGTTT+TGG - Chr8:22000621-22000640 MsG0880043287.01.T01:CDS 40.0%
!! AACAAGGTATTGAGGATGTG+GGG - Chr8:22000566-22000585 MsG0880043287.01.T01:CDS 40.0%
!! AGAACAAGGTATTGAGGATG+TGG - Chr8:22000564-22000583 MsG0880043287.01.T01:CDS 40.0%
!! GAACAAGGTATTGAGGATGT+GGG - Chr8:22000565-22000584 MsG0880043287.01.T01:CDS 40.0%
!!! AACGTCACTGATTTTGCATG+CGG + Chr8:22000337-22000356 None:intergenic 40.0%
CGTCACCAAGAAAATCCGAA+AGG + Chr8:22000417-22000436 None:intergenic 45.0%
GTGATTTCTAATGTGCGTGC+TGG + Chr8:22000154-22000173 None:intergenic 45.0%
TATTGAGGATGTGGGGTTGA+AGG - Chr8:22000573-22000592 MsG0880043287.01.T01:CDS 45.0%
! GAGTTATGGGTTGAGGGATT+GGG - Chr8:22000468-22000487 MsG0880043287.01.T01:CDS 45.0%
! TGAGTTATGGGTTGAGGGAT+TGG - Chr8:22000467-22000486 MsG0880043287.01.T01:CDS 45.0%
AGTGGCTAGGATAGCCCTTT+CGG - Chr8:22000399-22000418 MsG0880043287.01.T01:CDS 50.0%
GCTATCCTAGCCACTCCAAT+GGG + Chr8:22000394-22000413 None:intergenic 50.0%
GTGAGAAGGGTGACACAAGA+AGG - Chr8:22000523-22000542 MsG0880043287.01.T01:CDS 50.0%
TACAAAGGAGCTCGTCCCAT+TGG - Chr8:22000376-22000395 MsG0880043287.01.T01:CDS 50.0%
! TATGGGTTGAGGGATTGGGA+TGG - Chr8:22000472-22000491 MsG0880043287.01.T01:CDS 50.0%
!!! GTCACTGATTTTGCATGCGG+TGG + Chr8:22000334-22000353 None:intergenic 50.0%
GGCTATCCTAGCCACTCCAA+TGG + Chr8:22000395-22000414 None:intergenic 55.0%
GGTTGAGGGATTGGGATGGT+AGG - Chr8:22000476-22000495 MsG0880043287.01.T01:CDS 55.0%
TGAGGATGTGGGGTTGAAGG+TGG - Chr8:22000576-22000595 MsG0880043287.01.T01:CDS 55.0%
AGGAGCTCGTCCCATTGGAG+TGG - Chr8:22000381-22000400 MsG0880043287.01.T01:CDS 60.0%
CTCGTCCCATTGGAGTGGCT+AGG - Chr8:22000386-22000405 MsG0880043287.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 22000136 22000696 22000136 ID=MsG0880043287.01;Name=MsG0880043287.01
Chr8 mRNA 22000136 22000696 22000136 ID=MsG0880043287.01.T01;Parent=MsG0880043287.01;Name=MsG0880043287.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|186
Chr8 exon 22000136 22000696 22000136 ID=MsG0880043287.01.T01:exon:5831;Parent=MsG0880043287.01.T01
Chr8 CDS 22000136 22000696 22000136 ID=MsG0880043287.01.T01:cds;Parent=MsG0880043287.01.T01
Gene Sequence

>MsG0880043287.01.T01

ATGGAAGGAAAACAACCAGCACGCACATTAGAAATCACCATTATTTCCGGACAAAACATAAGTGTTGATCGAAATTCAAAGGGAGATGATATTTATGTTGTTGTTCGTGCGGAGTCTCTCAACTCTTGCACGACAAAAATGGTGAAGGAAAATGAGGGTTTACTTTCATGGAACGAAAAATTCTTATTAGATATTCCACCGCATGCAAAATCAGTGACGTTTGAGGTACAATGCAAGAAGTACAAAGGAGCTCGTCCCATTGGAGTGGCTAGGATAGCCCTTTCGGATTTTCTTGGTGACGATTTGTCGTTGCAAAGTGATGTTCAAACTTTGAGTTATGGGTTGAGGGATTGGGATGGTAGGAAAAATGGTGTTATTAATTTTAGTGTGAGAAGGGTGACACAAGAAGGTAATTTGTGTGTAGAAAAAGAACAAGGTATTGAGGATGTGGGGTTGAAGGTGGATGAGAAGAATTCAAATCATGTTGTCATTGGGATTCCAGTTTTGGGTTTTTTGTTTCTTTTGGGCTATTCTCTTTTCTCTTTTGTTCGTCGAAAGTAA

Protein sequence

>MsG0880043287.01.T01

MEGKQPARTLEITIISGQNISVDRNSKGDDIYVVVRAESLNSCTTKMVKENEGLLSWNEKFLLDIPPHAKSVTFEVQCKKYKGARPIGVARIALSDFLGDDLSLQSDVQTLSYGLRDWDGRKNGVINFSVRRVTQEGNLCVEKEQGIEDVGLKVDEKNSNHVVIGIPVLGFLFLLGYSLFSFVRRK*