AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019164.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019164.01.T01 MTR_4g024370 97.170 106 3 0 1 106 1 106 1.15e-72 216
MsG0480019164.01.T01 MTR_4g024370 97.170 106 3 0 1 106 1 106 1.15e-72 216
MsG0480019164.01.T01 MTR_5g011800 58.095 105 39 1 1 105 1 100 1.82e-37 126
MsG0480019164.01.T01 MTR_4g102220 56.731 104 36 1 1 104 1 95 7.04e-36 122
MsG0480019164.01.T01 MTR_5g011820 56.190 105 40 1 1 105 4 102 1.22e-33 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019164.01.T01 AT2G45360 64.286 98 26 1 1 98 1 89 1.20e-36 124
MsG0480019164.01.T01 AT3G60780 60.638 94 28 1 1 94 1 85 1.76e-32 113
MsG0480019164.01.T01 AT1G62840 53.846 104 39 1 1 104 1 95 3.15e-26 97.4
MsG0480019164.01.T01 AT1G12320 52.000 100 39 1 1 100 1 91 2.73e-23 89.7

Find 37 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAACATTCTACGATGAATT+TGG 0.250717 4:+15246405 None:intergenic
TATACATCATGAAGCTTGTT+TGG 0.316497 4:-15247155 None:intergenic
AGGCCAACACTTGCGGCTAT+TGG 0.324490 4:+15246370 None:intergenic
TGTAATCTTGACCCTTCATC+TGG 0.339388 4:+15246301 None:intergenic
TGTGAGAAATTCAAGGAATC+TGG 0.400122 4:-15246469 MsG0480019164.01.T01:CDS
GGGATACACCCATCTCGGCT+AGG 0.414592 4:+15246266 None:intergenic
TATGCAGTGTGAGAAATTCA+AGG 0.454346 4:-15246476 MsG0480019164.01.T01:intron
GATGAATTTGGTGTTCCATC+CGG 0.471577 4:+15246417 None:intergenic
GGATACACCCATCTCGGCTA+GGG 0.478620 4:+15246267 None:intergenic
CACAAAAGCCCTAGCCGAGA+TGG 0.481827 4:-15246275 MsG0480019164.01.T01:CDS
TTGAGGTTGAAAGGTGCTTA+CGG 0.505934 4:+15247089 None:intergenic
ATTGATCTTACTTGTTGTTG+AGG 0.510185 4:+15247072 None:intergenic
GCGGTTGCTGCTCGCAACAC+CGG 0.525122 4:-15246346 MsG0480019164.01.T01:CDS
TGTTGCGAGCAGCAACCGCT+AGG 0.535059 4:+15246350 None:intergenic
TCACCGAGAACTATCTCCGT+GGG 0.538377 4:+15246242 None:intergenic
TACTTGTTGTTGAGGTTGAA+AGG 0.550183 4:+15247080 None:intergenic
ACAAAAGCCCTAGCCGAGAT+GGG 0.552505 4:-15246274 MsG0480019164.01.T01:CDS
GGTGTTCCATCCGGCTGCCA+TGG 0.552686 4:+15246426 None:intergenic
CTTCTATCATCCATGGCAGC+CGG 0.557426 4:-15246436 MsG0480019164.01.T01:CDS
ATCGTAGAATGTTATTCCCA+CGG 0.567348 4:-15246397 MsG0480019164.01.T01:CDS
TATGCACACGTGTCTTGCAC+CGG 0.569145 4:+15246327 None:intergenic
GTGTGCATAGTCCCAGATGA+AGG 0.570105 4:-15246313 MsG0480019164.01.T01:CDS
AACCGCTAGGCCAACACTTG+CGG 0.573043 4:+15246363 None:intergenic
GGTCCAATAGCCGCAAGTGT+TGG 0.576902 4:-15246373 MsG0480019164.01.T01:CDS
GTAATCTTGACCCTTCATCT+GGG 0.578141 4:+15246302 None:intergenic
AGCTGAGCTTCTATCATCCA+TGG 0.578564 4:-15246443 MsG0480019164.01.T01:CDS
TATCATCCATGGCAGCCGGA+TGG 0.579782 4:-15246432 MsG0480019164.01.T01:CDS
TGCGGCTATTGGACCGCCGT+GGG 0.584383 4:+15246381 None:intergenic
TTGCGGCTATTGGACCGCCG+TGG 0.612852 4:+15246380 None:intergenic
CCGAGAACTATCTCCGTGGG+GGG 0.618245 4:+15246245 None:intergenic
TGTGCATAGTCCCAGATGAA+GGG 0.619561 4:-15246312 MsG0480019164.01.T01:CDS
CGAGAACTATCTCCGTGGGG+GGG 0.626603 4:+15246246 None:intergenic
AGCCGCAAGTGTTGGCCTAG+CGG 0.628784 4:-15246365 MsG0480019164.01.T01:CDS
CACCGAGAACTATCTCCGTG+GGG 0.644556 4:+15246243 None:intergenic
TTCACCGAGAACTATCTCCG+TGG 0.644732 4:+15246241 None:intergenic
ACCGAGAACTATCTCCGTGG+GGG 0.654535 4:+15246244 None:intergenic
GTAGAATGTTATTCCCACGG+CGG 0.814857 4:-15246394 MsG0480019164.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAATATATATTGAAAAAT+TGG + Chr4:15246486-15246505 None:intergenic 10.0%
!! TTAATATGGTATAATTATTT+GGG - Chr4:15246894-15246913 MsG0480019164.01.T01:intron 10.0%
!! TTTAATATGGTATAATTATT+TGG - Chr4:15246893-15246912 MsG0480019164.01.T01:intron 10.0%
!! AAAAGAAAAAATTTACAAAG+AGG + Chr4:15246458-15246477 None:intergenic 15.0%
!! AAAGAAAAAATTTACAAAGA+GGG + Chr4:15246457-15246476 None:intergenic 15.0%
!! AACTAAAATTGAAAAAAGAA+AGG + Chr4:15246433-15246452 None:intergenic 15.0%
!! AAGAAAAAAAATTATACAAG+AGG + Chr4:15246726-15246745 None:intergenic 15.0%
!! ACTAAAATTGAAAAAAGAAA+GGG + Chr4:15246432-15246451 None:intergenic 15.0%
!! AGAAAAAAAATTATACAAGA+GGG + Chr4:15246725-15246744 None:intergenic 15.0%
!! TAGTGATTATAATTTATTCT+TGG + Chr4:15246832-15246851 None:intergenic 15.0%
!!! AAATTGATGATGTTTTAATA+TGG - Chr4:15246880-15246899 MsG0480019164.01.T01:intron 15.0%
!!! TTTTAAGACAAATAACAACA+AGG + Chr4:15246861-15246880 None:intergenic 20.0%
! ATAACATTCTACGATGAATT+TGG + Chr4:15246997-15247016 None:intergenic 25.0%
ACGTAAACTTATGTGATTGA+AGG + Chr4:15246608-15246627 None:intergenic 30.0%
ATACAAGAGGGAAATGAAAA+TGG + Chr4:15246713-15246732 None:intergenic 30.0%
CATATTATGTGATGTGATTG+CGG + Chr4:15246775-15246794 None:intergenic 30.0%
CGTAAACTTATGTGATTGAA+GGG + Chr4:15246607-15246626 None:intergenic 30.0%
! AGTGTCTATGTAAGCTTTTA+AGG + Chr4:15246283-15246302 None:intergenic 30.0%
!! ATTGATCTTACTTGTTGTTG+AGG + Chr4:15246330-15246349 None:intergenic 30.0%
AAAGGGACAAAGAAAGAAGA+AGG + Chr4:15246415-15246434 None:intergenic 35.0%
AAGGGACAAAGAAAGAAGAA+GGG + Chr4:15246414-15246433 None:intergenic 35.0%
ATCGTAGAATGTTATTCCCA+CGG - Chr4:15247002-15247021 MsG0480019164.01.T01:intron 35.0%
CGCAATCACATCACATAATA+TGG - Chr4:15246773-15246792 MsG0480019164.01.T01:intron 35.0%
GGGAAATGAAAATGGAAAAG+TGG + Chr4:15246705-15246724 None:intergenic 35.0%
TATGCAGTGTGAGAAATTCA+AGG - Chr4:15246923-15246942 MsG0480019164.01.T01:intron 35.0%
TGTGAGAAATTCAAGGAATC+TGG - Chr4:15246930-15246949 MsG0480019164.01.T01:intron 35.0%
!! TACTTGTTGTTGAGGTTGAA+AGG + Chr4:15246322-15246341 None:intergenic 35.0%
!!! TAAGCTTTTAAGGCAGTTTC+TGG + Chr4:15246273-15246292 None:intergenic 35.0%
GTAATCTTGACCCTTCATCT+GGG + Chr4:15247100-15247119 None:intergenic 40.0%
TGTAATCTTGACCCTTCATC+TGG + Chr4:15247101-15247120 None:intergenic 40.0%
!! GATGAATTTGGTGTTCCATC+CGG + Chr4:15246985-15247004 None:intergenic 40.0%
!! TTGAGGTTGAAAGGTGCTTA+CGG + Chr4:15246313-15246332 None:intergenic 40.0%
AGCTGAGCTTCTATCATCCA+TGG - Chr4:15246956-15246975 MsG0480019164.01.T01:intron 45.0%
GTAGAATGTTATTCCCACGG+CGG - Chr4:15247005-15247024 MsG0480019164.01.T01:intron 45.0%
TGTGCATAGTCCCAGATGAA+GGG - Chr4:15247087-15247106 MsG0480019164.01.T01:CDS 45.0%
ACAAAAGCCCTAGCCGAGAT+GGG - Chr4:15247125-15247144 MsG0480019164.01.T01:CDS 50.0%
CTTCTATCATCCATGGCAGC+CGG - Chr4:15246963-15246982 MsG0480019164.01.T01:intron 50.0%
GTGTGCATAGTCCCAGATGA+AGG - Chr4:15247086-15247105 MsG0480019164.01.T01:CDS 50.0%
TATGCACACGTGTCTTGCAC+CGG + Chr4:15247075-15247094 None:intergenic 50.0%
AACCGCTAGGCCAACACTTG+CGG + Chr4:15247039-15247058 None:intergenic 55.0%
AGGCCAACACTTGCGGCTAT+TGG + Chr4:15247032-15247051 None:intergenic 55.0%
CACAAAAGCCCTAGCCGAGA+TGG - Chr4:15247124-15247143 MsG0480019164.01.T01:CDS 55.0%
GGATACACCCATCTCGGCTA+GGG + Chr4:15247135-15247154 None:intergenic 55.0%
GGTCCAATAGCCGCAAGTGT+TGG - Chr4:15247026-15247045 MsG0480019164.01.T01:intron 55.0%
TATCATCCATGGCAGCCGGA+TGG - Chr4:15246967-15246986 MsG0480019164.01.T01:intron 55.0%
AGATGGGTGTATCCCCCCCA+CGG - Chr4:15247141-15247160 MsG0480019164.01.T01:CDS 60.0%
GGGATACACCCATCTCGGCT+AGG + Chr4:15247136-15247155 None:intergenic 60.0%
TGGGGGGGATACACCCATCT+CGG + Chr4:15247141-15247160 None:intergenic 60.0%
TGTTGCGAGCAGCAACCGCT+AGG + Chr4:15247052-15247071 None:intergenic 60.0%
!! AGCCGCAAGTGTTGGCCTAG+CGG - Chr4:15247034-15247053 MsG0480019164.01.T01:intron 60.0%
GCGGTTGCTGCTCGCAACAC+CGG - Chr4:15247053-15247072 MsG0480019164.01.T01:intron 65.0%
GGTGTTCCATCCGGCTGCCA+TGG + Chr4:15246976-15246995 None:intergenic 65.0%
TGCGGCTATTGGACCGCCGT+GGG + Chr4:15247021-15247040 None:intergenic 65.0%
TTGCGGCTATTGGACCGCCG+TGG + Chr4:15247022-15247041 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 15246252 15247169 15246252 ID=MsG0480019164.01;Name=MsG0480019164.01
Chr4 mRNA 15246252 15247169 15246252 ID=MsG0480019164.01.T01;Parent=MsG0480019164.01;Name=MsG0480019164.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|2|0|108
Chr4 exon 15247083 15247169 15247083 ID=MsG0480019164.01.T01:exon:4949;Parent=MsG0480019164.01.T01
Chr4 exon 15246252 15246491 15246252 ID=MsG0480019164.01.T01:exon:4948;Parent=MsG0480019164.01.T01
Chr4 CDS 15247083 15247169 15247083 ID=MsG0480019164.01.T01:cds;Parent=MsG0480019164.01.T01
Chr4 CDS 15246252 15246491 15246252 ID=MsG0480019164.01.T01:cds;Parent=MsG0480019164.01.T01
Gene Sequence

>MsG0480019164.01.T01

ATGAAGCTTGTTTGGTCTCCAGAAACTGCCTTAAAAGCTTACATAGACACTGTTAAATCCGTAAGCACCTTTCAACCTCAACAACAATGTGAGAAATTCAAGGAATCTGGAGTAGCTGAGCTTCTATCATCCATGGCAGCCGGATGGAACACCAAATTCATCGTAGAATGTTATTCCCACGGCGGTCCAATAGCCGCAAGTGTTGGCCTAGCGGTTGCTGCTCGCAACACCGGTGCAAGACACGTGTGCATAGTCCCAGATGAAGGGTCAAGATTACAATACACAAAAGCCCTAGCCGAGATGGGTGTATCCCCCCCACGGAGATAG

Protein sequence

>MsG0480019164.01.T01

MKLVWSPETALKAYIDTVKSVSTFQPQQQCEKFKESGVAELLSSMAAGWNTKFIVECYSHGGPIAASVGLAVAARNTGARHVCIVPDEGSRLQYTKALAEMGVSPPRR*