AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047363.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047363.01.T01 MTR_4g125600 95.868 121 1 1 1 121 1 117 4.51e-74 216
MsG0880047363.01.T01 MTR_3g104950 57.724 123 45 5 1 120 1 119 1.57e-35 118
MsG0880047363.01.T01 MTR_1g015290 58.730 126 44 5 1 120 1 124 3.59e-34 115
MsG0880047363.01.T01 MTR_2g041260 65.289 121 39 3 1 120 1 119 7.36e-33 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047363.01.T01 AT2G06520 57.724 123 41 4 1 120 1 115 3.99e-29 102

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGCTCAGCATCGTTTC+TGG 0.146677 8:-85274190 MsG0880047363.01.T01:CDS
GCTATTCTTGGTGCTGTTAT+TGG 0.188543 8:-85274151 MsG0880047363.01.T01:CDS
TTCTTGCTGGCACAAGTTAA+TGG 0.260311 8:+85274427 None:intergenic
TCGCTTAACAGGATCGAAAT+TGG 0.294106 8:+85274123 None:intergenic
GTTGTGGTTACTGCTATTCT+TGG 0.319417 8:-85274163 MsG0880047363.01.T01:CDS
ATTCATCAACCTCGCTTAAC+AGG 0.324240 8:+85274112 None:intergenic
TGGTGCCTCTAGCTTCTTGC+TGG 0.342131 8:+85274414 None:intergenic
TGTCACAAGTTTCTCTTTCA+AGG 0.346815 8:+85274297 None:intergenic
GTTGCTGCCACAGACTTTGA+TGG 0.351954 8:+85274352 None:intergenic
TTCAACTTCCACAATTTCAA+TGG 0.410680 8:-85274455 MsG0880047363.01.T01:CDS
AACCTCTCAAGCATTCTTGA+AGG 0.466332 8:-85274392 MsG0880047363.01.T01:CDS
TTTGATGGCTTCAAAGTCAA+TGG 0.476094 8:+85274367 None:intergenic
TTGAAAGAGAAACTTGTGAC+AGG 0.488854 8:-85274295 MsG0880047363.01.T01:CDS
GTAGCAGCTTCAGCCACGTC+AGG 0.500301 8:+85274235 None:intergenic
TTCAAGAATGCTTGAGAGGT+TGG 0.501532 8:+85274394 None:intergenic
TCACTACAAACCTTGCGTTA+CGG 0.517131 8:+85274326 None:intergenic
TGGCACAGCCATTGAAATTG+TGG 0.527378 8:+85274447 None:intergenic
TTCAATGATTTCTCCTGACG+TGG 0.557151 8:-85274248 MsG0880047363.01.T01:CDS
CATCGTTTCTGGTGGTGTTG+TGG 0.559998 8:-85274179 MsG0880047363.01.T01:CDS
AGCAAGAAATTCTTGAGAGA+AGG 0.566334 8:+85274205 None:intergenic
TTGCTCAGCATCGTTTCTGG+TGG 0.567414 8:-85274187 MsG0880047363.01.T01:CDS
AATTTCGATCCTGTTAAGCG+AGG 0.632764 8:-85274121 MsG0880047363.01.T01:CDS
CAGCAACAAACCGTAACGCA+AGG 0.648127 8:-85274336 MsG0880047363.01.T01:CDS
TGAAAGAGAAACTTGTGACA+GGG 0.650173 8:-85274294 MsG0880047363.01.T01:CDS
TTTGAAGCCATCAAAGTCTG+TGG 0.657555 8:-85274359 MsG0880047363.01.T01:CDS
TGCCTTCAAGAATGCTTGAG+AGG 0.659518 8:+85274390 None:intergenic
TTGTGCCAGCAAGAAGCTAG+AGG 0.660281 8:-85274419 MsG0880047363.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTCAACTTCCACAATTTCAA+TGG - Chr8:85274122-85274141 MsG0880047363.01.T01:CDS 30.0%
AGCAAGAAATTCTTGAGAGA+AGG + Chr8:85274375-85274394 None:intergenic 35.0%
TGAAAGAGAAACTTGTGACA+GGG - Chr8:85274283-85274302 MsG0880047363.01.T01:CDS 35.0%
TGTCACAAGTTTCTCTTTCA+AGG + Chr8:85274283-85274302 None:intergenic 35.0%
TTGAAAGAGAAACTTGTGAC+AGG - Chr8:85274282-85274301 MsG0880047363.01.T01:CDS 35.0%
! TTTGATGGCTTCAAAGTCAA+TGG + Chr8:85274213-85274232 None:intergenic 35.0%
AACCTCTCAAGCATTCTTGA+AGG - Chr8:85274185-85274204 MsG0880047363.01.T01:CDS 40.0%
AATTTCGATCCTGTTAAGCG+AGG - Chr8:85274456-85274475 MsG0880047363.01.T01:CDS 40.0%
GTTGTGGTTACTGCTATTCT+TGG - Chr8:85274414-85274433 MsG0880047363.01.T01:CDS 40.0%
TCACTACAAACCTTGCGTTA+CGG + Chr8:85274254-85274273 None:intergenic 40.0%
TCGCTTAACAGGATCGAAAT+TGG + Chr8:85274457-85274476 None:intergenic 40.0%
TTCAAGAATGCTTGAGAGGT+TGG + Chr8:85274186-85274205 None:intergenic 40.0%
TTCAATGATTTCTCCTGACG+TGG - Chr8:85274329-85274348 MsG0880047363.01.T01:CDS 40.0%
TTCTTGCTGGCACAAGTTAA+TGG + Chr8:85274153-85274172 None:intergenic 40.0%
! TTTGAAGCCATCAAAGTCTG+TGG - Chr8:85274218-85274237 MsG0880047363.01.T01:CDS 40.0%
!! GCTATTCTTGGTGCTGTTAT+TGG - Chr8:85274426-85274445 MsG0880047363.01.T01:CDS 40.0%
TGCCTTCAAGAATGCTTGAG+AGG + Chr8:85274190-85274209 None:intergenic 45.0%
TGGCACAGCCATTGAAATTG+TGG + Chr8:85274133-85274152 None:intergenic 45.0%
TTCTTGCTCAGCATCGTTTC+TGG - Chr8:85274387-85274406 MsG0880047363.01.T01:CDS 45.0%
CAGCAACAAACCGTAACGCA+AGG - Chr8:85274241-85274260 MsG0880047363.01.T01:CDS 50.0%
GTTGCTGCCACAGACTTTGA+TGG + Chr8:85274228-85274247 None:intergenic 50.0%
TTGTGCCAGCAAGAAGCTAG+AGG - Chr8:85274158-85274177 MsG0880047363.01.T01:CDS 50.0%
! TTGCTCAGCATCGTTTCTGG+TGG - Chr8:85274390-85274409 MsG0880047363.01.T01:CDS 50.0%
!! CATCGTTTCTGGTGGTGTTG+TGG - Chr8:85274398-85274417 MsG0880047363.01.T01:CDS 50.0%
! TGGTGCCTCTAGCTTCTTGC+TGG + Chr8:85274166-85274185 None:intergenic 55.0%
GTAGCAGCTTCAGCCACGTC+AGG + Chr8:85274345-85274364 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 85274117 85274482 85274117 ID=MsG0880047363.01;Name=MsG0880047363.01
Chr8 mRNA 85274117 85274482 85274117 ID=MsG0880047363.01.T01;Parent=MsG0880047363.01;Name=MsG0880047363.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|121
Chr8 exon 85274117 85274482 85274117 ID=MsG0880047363.01.T01:exon:36380;Parent=MsG0880047363.01.T01
Chr8 CDS 85274117 85274482 85274117 ID=MsG0880047363.01.T01:cds;Parent=MsG0880047363.01.T01
Gene Sequence

>MsG0880047363.01.T01

ATGGCTTCAACTTCCACAATTTCAATGGCTGTGCCATTAACTTGTGCCAGCAAGAAGCTAGAGGCACCAACCTCTCAAGCATTCTTGAAGGCACCATTGACTTTGAAGCCATCAAAGTCTGTGGCAGCAACAAACCGTAACGCAAGGTTTGTAGTGAAAGCATCCTTGAAAGAGAAACTTGTGACAGGGTTAACTGCAGCTGCATTGACAGCTTCAATGATTTCTCCTGACGTGGCTGAAGCTGCTACTGTTTCACCTTCTCTCAAGAATTTCTTGCTCAGCATCGTTTCTGGTGGTGTTGTGGTTACTGCTATTCTTGGTGCTGTTATTGGTGTTTCCAATTTCGATCCTGTTAAGCGAGGTTGA

Protein sequence

>MsG0880047363.01.T01

MASTSTISMAVPLTCASKKLEAPTSQAFLKAPLTLKPSKSVAATNRNARFVVKASLKEKLVTGLTAAALTASMISPDVAEAATVSPSLKNFLLSIVSGGVVVTAILGAVIGVSNFDPVKRG*