AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040894.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040894.01.T01 MTR_7g061255 88.679 53 6 0 22 74 21 73 1.92e-28 99.0
MsG0780040894.01.T01 MTR_7g100570 88.679 53 6 0 22 74 75 127 6.90e-28 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040894.01.T01 AT3G53470 66.102 59 20 0 19 77 64 122 1.30e-21 82.0
MsG0780040894.01.T01 AT3G53470 66.102 59 20 0 19 77 63 121 1.39e-21 82.0

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAGCTCACATATTTAT+TGG 0.198892 7:+83950691 None:intergenic
GATCTTTCTGTTCTCTTGAT+AGG 0.273479 7:+83950629 None:intergenic
AAAGATCAAACCTCAAATTC+TGG 0.301138 7:-83950613 MsG0780040894.01.T01:CDS
TGATAAATCATCAACGGATT+GGG 0.428760 7:-83950772 MsG0780040894.01.T01:CDS
AACTCTTCGTTCAATGTCTC+AGG 0.439058 7:-83950795 None:intergenic
GTGATAAATCATCAACGGAT+TGG 0.467245 7:-83950773 MsG0780040894.01.T01:CDS
TAGGACTAGTCCAGAATTTG+AGG 0.468478 7:+83950603 None:intergenic
ATGGTCAAACTTCAAGAAGC+AGG 0.484213 7:-83950742 MsG0780040894.01.T01:CDS
AATATGTGAGCTGGAATCCT+AGG 0.492726 7:-83950685 MsG0780040894.01.T01:CDS
TCTCCAATAAATATGTGAGC+TGG 0.494159 7:-83950694 MsG0780040894.01.T01:CDS
GGATCAACTTCTTCAGAAAG+TGG 0.503418 7:+83950650 None:intergenic
CAACGGATTGGGACAAGGCA+TGG 0.546580 7:-83950761 MsG0780040894.01.T01:CDS
ATCATCAACGGATTGGGACA+AGG 0.594053 7:-83950766 MsG0780040894.01.T01:CDS
AGTGGAAAATCTGAACGCCT+AGG 0.596558 7:+83950668 None:intergenic
CTCAGGTGATAAATCATCAA+CGG 0.602810 7:-83950778 MsG0780040894.01.T01:CDS
TGGTCAAACTTCAAGAAGCA+GGG 0.615004 7:-83950741 MsG0780040894.01.T01:CDS
GCCCCACCAAGAGTAAAAGT+AGG 0.658878 7:+83950584 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGATCAAACCTCAAATTC+TGG - Chr7:83950737-83950756 MsG0780040894.01.T01:CDS 30.0%
ATTCCAGCTCACATATTTAT+TGG + Chr7:83950662-83950681 None:intergenic 30.0%
! TGATAAATCATCAACGGATT+GGG - Chr7:83950578-83950597 MsG0780040894.01.T01:CDS 30.0%
CTCAGGTGATAAATCATCAA+CGG - Chr7:83950572-83950591 MsG0780040894.01.T01:CDS 35.0%
GATCTTTCTGTTCTCTTGAT+AGG + Chr7:83950724-83950743 None:intergenic 35.0%
GTGATAAATCATCAACGGAT+TGG - Chr7:83950577-83950596 MsG0780040894.01.T01:CDS 35.0%
TCTCCAATAAATATGTGAGC+TGG - Chr7:83950656-83950675 MsG0780040894.01.T01:CDS 35.0%
!! ACTAGTCCTACTTTTACTCT+TGG - Chr7:83950760-83950779 MsG0780040894.01.T01:CDS 35.0%
AATATGTGAGCTGGAATCCT+AGG - Chr7:83950665-83950684 MsG0780040894.01.T01:CDS 40.0%
ATGGTCAAACTTCAAGAAGC+AGG - Chr7:83950608-83950627 MsG0780040894.01.T01:CDS 40.0%
GGATCAACTTCTTCAGAAAG+TGG + Chr7:83950703-83950722 None:intergenic 40.0%
TGGTCAAACTTCAAGAAGCA+GGG - Chr7:83950609-83950628 MsG0780040894.01.T01:CDS 40.0%
!! AGTCCTACTTTTACTCTTGG+TGG - Chr7:83950763-83950782 MsG0780040894.01.T01:CDS 40.0%
!! GTCCTACTTTTACTCTTGGT+GGG - Chr7:83950764-83950783 MsG0780040894.01.T01:CDS 40.0%
!! TAGGACTAGTCCAGAATTTG+AGG + Chr7:83950750-83950769 None:intergenic 40.0%
!! TCCTACTTTTACTCTTGGTG+GGG - Chr7:83950765-83950784 MsG0780040894.01.T01:CDS 40.0%
AGTGGAAAATCTGAACGCCT+AGG + Chr7:83950685-83950704 None:intergenic 45.0%
!! ATCATCAACGGATTGGGACA+AGG - Chr7:83950584-83950603 MsG0780040894.01.T01:CDS 45.0%
GCCCCACCAAGAGTAAAAGT+AGG + Chr7:83950769-83950788 None:intergenic 50.0%
!! CAACGGATTGGGACAAGGCA+TGG - Chr7:83950589-83950608 MsG0780040894.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83950568 83950804 83950568 ID=MsG0780040894.01;Name=MsG0780040894.01
Chr7 mRNA 83950568 83950804 83950568 ID=MsG0780040894.01.T01;Parent=MsG0780040894.01;Name=MsG0780040894.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|0|78
Chr7 exon 83950568 83950804 83950568 ID=MsG0780040894.01.T01:exon:11485;Parent=MsG0780040894.01.T01
Chr7 CDS 83950568 83950804 83950568 ID=MsG0780040894.01.T01:cds;Parent=MsG0780040894.01.T01
Gene Sequence

>MsG0780040894.01.T01

ATGTCTCAGGTGATAAATCATCAACGGATTGGGACAAGGCATGGTCAAACTTCAAGAAGCAGGGAAAAAAAACTCTCTTCTCAATTTTTCTCCAATAAATATGTGAGCTGGAATCCTAGGCGTTCAGATTTTCCACTTTCTGAAGAAGTTGATCCTATCAAGAGAACAGAAAGATCAAACCTCAAATTCTGGACTAGTCCTACTTTTACTCTTGGTGGGGCACTTATATTCTCATAG

Protein sequence

>MsG0780040894.01.T01

MSQVINHQRIGTRHGQTSRSREKKLSSQFFSNKYVSWNPRRSDFPLSEEVDPIKRTERSNLKFWTSPTFTLGGALIFS*