AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022465.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022465.01.T01 MTR_4g088845 62.712 472 124 1 1 420 1 472 0.0 584
MsG0480022465.01.T01 MTR_7g076760 59.789 475 131 5 1 416 1 474 0.0 558
MsG0480022465.01.T01 MTR_7g076570 60.638 470 129 3 1 420 29 492 0.0 553
MsG0480022465.01.T01 MTR_7g050670 54.352 471 159 6 1 416 1 470 5.50e-168 480
MsG0480022465.01.T01 MTR_0049s0110 24.648 426 255 15 20 395 6 415 5.51e-14 73.6
MsG0480022465.01.T01 MTR_1g011430 24.370 357 218 11 24 363 12 333 9.11e-14 72.8
MsG0480022465.01.T01 MTR_0049s0110 25.503 298 197 10 16 299 5 291 1.89e-12 68.6
MsG0480022465.01.T01 MTR_0049s0110 25.503 298 197 10 16 299 5 291 2.22e-12 68.2
MsG0480022465.01.T01 MTR_0049s0110 28.342 187 124 4 20 198 6 190 2.26e-12 67.4
MsG0480022465.01.T01 MTR_1g021820 24.535 269 168 7 5 265 14 255 3.98e-11 65.1
MsG0480022465.01.T01 MTR_1g013560 23.194 263 162 7 13 262 13 248 7.32e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022465.01.T01 AT1G61330 24.938 405 240 8 24 365 15 418 2.27e-30 122
MsG0480022465.01.T01 AT1G61320 24.466 421 253 10 9 365 11 430 1.27e-29 120

Find 60 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAATTTCAATTTCTTAA+TGG 0.178800 4:+72858711 None:intergenic
TTTGCAAAGAGCAATAAATT+TGG 0.216126 4:-72856059 MsG0480022465.01.T01:CDS
TTACCTCAGTTAATTGGTTT+TGG 0.246189 4:+72859301 None:intergenic
TATTGGTAATAAAGAGAAAA+TGG 0.261048 4:+72859227 None:intergenic
CACTTGGAACCTTTGGTAAC+TGG 0.301004 4:+72858851 None:intergenic
AGCAATAAATTTGGAGAGGT+TGG 0.308631 4:-72856050 MsG0480022465.01.T01:CDS
GATTCTCCCGATGATCTTCC+TGG 0.312743 4:-72859264 MsG0480022465.01.T01:CDS
TGCAAGCCACTTGGAACCTT+TGG 0.328566 4:+72858844 None:intergenic
GTTGTTGATCGTATCGTAAA+TGG 0.350712 4:+72859115 None:intergenic
TATTTAACCTTGGACTGTAT+TGG 0.356106 4:-72856198 MsG0480022465.01.T01:CDS
AGCCAGCATCTTGGATGGAT+TGG 0.393452 4:+72855881 None:intergenic
ATCATCGGGAGAATCAGTTT+TGG 0.406424 4:+72859272 None:intergenic
AATTGAGAATCGCTTGTTGC+CGG 0.419042 4:-72858663 MsG0480022465.01.T01:CDS
CATTTATTCAATTCGCATGA+AGG 0.444100 4:-72859072 MsG0480022465.01.T01:CDS
ACGATACGATCAACAACATA+TGG 0.447906 4:-72859109 MsG0480022465.01.T01:CDS
TTGCAGAGGCGATAAACCTT+TGG 0.456686 4:+72856016 None:intergenic
CACATCACTGAAGATATGTT+TGG 0.460765 4:-72858790 MsG0480022465.01.T01:CDS
CAGAGGCGATAAACCTTTGG+TGG 0.476365 4:+72856019 None:intergenic
ATTGAGAATCGCTTGTTGCC+GGG 0.476391 4:-72858662 MsG0480022465.01.T01:CDS
TACAGGTGTAGTGGTGAGAA+TGG 0.478938 4:+72858456 None:intergenic
CACTACACCTGTATTTATTG+AGG 0.480671 4:-72858446 MsG0480022465.01.T01:intron
GTTACAAATATGCACTCAAA+AGG 0.480867 4:-72858983 MsG0480022465.01.T01:CDS
TCAAAGGACACAATTGTATG+AGG 0.482482 4:-72858560 MsG0480022465.01.T01:CDS
ATTCTCCCGATGATCTTCCT+GGG 0.484154 4:-72859263 MsG0480022465.01.T01:CDS
AGTAAAGCTTCGATTCCAAT+GGG 0.491578 4:+72859015 None:intergenic
TTTCAATCTCTTTCAGATCC+CGG 0.493103 4:+72858644 None:intergenic
TCTGTATATAGCCAGCATCT+TGG 0.495828 4:+72855872 None:intergenic
AATGATTATATATTTAACCT+TGG 0.505018 4:-72856208 MsG0480022465.01.T01:CDS
TTATATACCTCAATAAATAC+AGG 0.505788 4:+72858439 None:intergenic
CTCCTATGTACAAAAGATCA+TGG 0.506236 4:-72859173 MsG0480022465.01.T01:CDS
AAGTAAAGCTTCGATTCCAA+TGG 0.510910 4:+72859014 None:intergenic
CTACTTCGAGTATACTAAGC+TGG 0.518883 4:-72858509 MsG0480022465.01.T01:CDS
ATATCCCCAGGAAGATCATC+GGG 0.521924 4:+72859258 None:intergenic
CTCAATAAATACAGGTGTAG+TGG 0.530505 4:+72858447 None:intergenic
AAGTTACATGTCAATCCCAT+TGG 0.538150 4:-72859030 MsG0480022465.01.T01:CDS
GTCCAATCCATCCAAGATGC+TGG 0.540343 4:-72855883 MsG0480022465.01.T01:CDS
CAATAGTTCGAATTGTTCAA+AGG 0.540408 4:-72858576 MsG0480022465.01.T01:CDS
TCGAAGCTTTACTTAAGAGA+TGG 0.540693 4:-72859005 MsG0480022465.01.T01:CDS
GCATTTGCCAATACAGTCCA+AGG 0.542064 4:+72856191 None:intergenic
TAGCTGCAATTGTCAAGTTG+AGG 0.548570 4:+72856160 None:intergenic
GAGAGCATCAACAAGGGCCG+TGG 0.553145 4:-72855945 MsG0480022465.01.T01:CDS
TATATAGCCAGCATCTTGGA+TGG 0.554906 4:+72855876 None:intergenic
GTTGGAGTTAATTCCACCAA+AGG 0.563134 4:-72856032 MsG0480022465.01.T01:CDS
GAGTTGCATCTTTACCGACC+TGG 0.565040 4:-72858946 MsG0480022465.01.T01:CDS
ATTTACTCAACTCAAAGTTG+TGG 0.581039 4:-72856134 MsG0480022465.01.T01:CDS
GATATCCCCAGGAAGATCAT+CGG 0.582925 4:+72859257 None:intergenic
ATGGTTTGAATGATATCCCC+AGG 0.585279 4:+72859246 None:intergenic
AAAGAGCAATAAATTTGGAG+AGG 0.594988 4:-72856054 MsG0480022465.01.T01:CDS
GCATCTTGGATGGATTGGAC+TGG 0.600480 4:+72855886 None:intergenic
GTTCTTGGAGAGCATCAACA+AGG 0.603617 4:-72855952 MsG0480022465.01.T01:CDS
ATGTGCGATCCAGTTACCAA+AGG 0.604288 4:-72858860 MsG0480022465.01.T01:CDS
CAAACATATCTTCAGTGATG+TGG 0.613965 4:+72858791 None:intergenic
ACATATCTTCAGTGATGTGG+AGG 0.615616 4:+72858794 None:intergenic
ATATTTATGTAACTTTACCA+CGG 0.623225 4:+72855928 None:intergenic
CTAAATTCAGTTGTGAAACC+AGG 0.629922 4:+72858928 None:intergenic
TTCTCCCGATGATCTTCCTG+GGG 0.653681 4:-72859262 MsG0480022465.01.T01:CDS
TTCTTGGAGAGCATCAACAA+GGG 0.661655 4:-72855951 MsG0480022465.01.T01:CDS
CAGTTACCAAAGGTTCCAAG+TGG 0.674160 4:-72858850 MsG0480022465.01.T01:CDS
ATTCAGTTGTGAAACCAGGT+CGG 0.683624 4:+72858932 None:intergenic
AAACCAAAACCAATTAACTG+AGG 0.694893 4:-72859304 MsG0480022465.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+GGG + Chr4:72857515-72857496 None:intergenic 0.0%
!! TATATATATATATATATATA+TGG + Chr4:72857514-72857495 None:intergenic 0.0%
!! AATTAATTTCAATTTCTTAA+TGG + Chr4:72856472-72856491 None:intergenic 10.0%
!! TTGAAATAAATAAATAATAG+AGG - Chr4:72858878-72858897 MsG0480022465.01.T01:CDS 10.0%
!!! AAAAAGGTTTTTTTAAAAAA+TGG - Chr4:72858178-72858197 MsG0480022465.01.T01:intron 10.0%
!!! AATTTTTTCAACTAAAAATA+TGG + Chr4:72857776-72857795 None:intergenic 10.0%
!!! CATTTTAAAACAATTTTTTT+TGG + Chr4:72857257-72857276 None:intergenic 10.0%
!! AAATTATCCTTATTAACTAT+TGG + Chr4:72857384-72857403 None:intergenic 15.0%
!! AACCAATAAAAATTATCTTT+AGG - Chr4:72858471-72858490 MsG0480022465.01.T01:CDS 15.0%
!! AATAGTTAATAAGGATAATT+TGG - Chr4:72857383-72857402 MsG0480022465.01.T01:intron 15.0%
!! AATGATTATATATTTAACCT+TGG - Chr4:72858972-72858991 MsG0480022465.01.T01:CDS 15.0%
!! CAAACACTTTATATAAAAAT+GGG + Chr4:72857972-72857991 None:intergenic 15.0%
!! GTTTAATAATTTATACATGT+TGG + Chr4:72856913-72856932 None:intergenic 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG + Chr4:72857360-72857379 None:intergenic 15.0%
!! AAAAAAAAAATGAATTGCTC+AGG + Chr4:72858919-72858938 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG - Chr4:72857374-72857393 MsG0480022465.01.T01:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + Chr4:72857297-72857316 None:intergenic 20.0%
!! ATAATTACTCAATAATCCAT+TGG + Chr4:72858635-72858654 None:intergenic 20.0%
!! ATATTTATGTAACTTTACCA+CGG + Chr4:72859255-72859274 None:intergenic 20.0%
!! ATTATTGAGTAATTATGTCT+AGG - Chr4:72858639-72858658 MsG0480022465.01.T01:CDS 20.0%
!! ATTCTCAACAAATTAGTTAA+CGG - Chr4:72857127-72857146 MsG0480022465.01.T01:intron 20.0%
!! CAAGTTAAAAACACTAAAAT+TGG - Chr4:72856293-72856312 MsG0480022465.01.T01:intron 20.0%
!! GCAAACACTTTATATAAAAA+TGG + Chr4:72857973-72857992 None:intergenic 20.0%
!! GGTAACTATTAATTAATTGT+TGG - Chr4:72857148-72857167 MsG0480022465.01.T01:intron 20.0%
!! TATATATTAGGAAAAACATG+CGG + Chr4:72857202-72857221 None:intergenic 20.0%
!! TTATATACCTCAATAAATAC+AGG + Chr4:72856744-72856763 None:intergenic 20.0%
!!! CAAGTTGATTAAAAAATTCA+TGG - Chr4:72858116-72858135 MsG0480022465.01.T01:intron 20.0%
!!! CACGTAAAGATAATTTTTAT+TGG + Chr4:72858476-72858495 None:intergenic 20.0%
!!! TAATTTATACATGTTGGTAA+AGG + Chr4:72856907-72856926 None:intergenic 20.0%
!!! TATTGGTAATAAAGAGAAAA+TGG + Chr4:72855956-72855975 None:intergenic 20.0%
!!! TTTAATCTTCTTGTTTTTCT+AGG + Chr4:72858028-72858047 None:intergenic 20.0%
!!! TTTGTTTAGCTTTACTTTTT+TGG + Chr4:72858001-72858020 None:intergenic 20.0%
!!! TTTTATTTACCTCAGTTAAT+TGG + Chr4:72855888-72855907 None:intergenic 20.0%
! AATGATTAAGAGAAAGAGAA+TGG + Chr4:72857417-72857436 None:intergenic 25.0%
! AATTGTTTATTCCAATCCAA+CGG + Chr4:72857622-72857641 None:intergenic 25.0%
! AGCTTCAATTATTTATGCAT+GGG - Chr4:72857013-72857032 MsG0480022465.01.T01:intron 25.0%
! TAAAAAATTGAACGAAGAGT+TGG + Chr4:72856570-72856589 None:intergenic 25.0%
! TCTAAAAAATTGTCCATCAT+TGG - Chr4:72857071-72857090 MsG0480022465.01.T01:intron 25.0%
! TCTCAAAGGAATAAAAATGT+TGG + Chr4:72857839-72857858 None:intergenic 25.0%
! TGGAGTTGAAAAACTAAAAA+AGG + Chr4:72857746-72857765 None:intergenic 25.0%
! TTTGCAAAGAGCAATAAATT+TGG - Chr4:72859121-72859140 MsG0480022465.01.T01:CDS 25.0%
!! ACTAAAAATATGGACTTTTC+TGG + Chr4:72857766-72857785 None:intergenic 25.0%
!! CAAAAAAGATTTTCGTATGT+TGG + Chr4:72859209-72859228 None:intergenic 25.0%
!! CATAATCGTCAATTTTTGTT+TGG - Chr4:72856033-72856052 MsG0480022465.01.T01:CDS 25.0%
!! CGCCTAAAGATAATTTTTAT+TGG + Chr4:72858584-72858603 None:intergenic 25.0%
!! GATTAAAATTAGCTTAAGCT+TGG - Chr4:72858041-72858060 MsG0480022465.01.T01:intron 25.0%
!! GTGGAAACAAATATCTTTTA+GGG - Chr4:72858072-72858091 MsG0480022465.01.T01:intron 25.0%
!! GTTACAATTGCATCTTTTAT+TGG + Chr4:72855973-72855992 None:intergenic 25.0%
!! TTCTGTTGATTAGAAGAATA+AGG - Chr4:72858220-72858239 MsG0480022465.01.T01:intron 25.0%
!!! ACGAAAATCTTTTTTGTTCT+TGG - Chr4:72859213-72859232 MsG0480022465.01.T01:CDS 25.0%
!!! ACTAATTTGTTGAGAATGAT+TGG + Chr4:72857124-72857143 None:intergenic 25.0%
!!! CTTCGTTCAATTTTTTATCA+TGG - Chr4:72856573-72856592 MsG0480022465.01.T01:intron 25.0%
!!! TGGACAATTTTTTAGAAACA+TGG + Chr4:72857067-72857086 None:intergenic 25.0%
!!! TTCAAATTTGTGTTAGCTTT+AGG - Chr4:72858141-72858160 MsG0480022465.01.T01:intron 25.0%
AAACCAAAACCAATTAACTG+AGG - Chr4:72855876-72855895 MsG0480022465.01.T01:CDS 30.0%
AAACGGATGGAGTATATATT+AGG + Chr4:72857214-72857233 None:intergenic 30.0%
AAAGAGCAATAAATTTGGAG+AGG - Chr4:72859126-72859145 MsG0480022465.01.T01:CDS 30.0%
AAAGAGGAAGAAAATCTCAA+AGG + Chr4:72857853-72857872 None:intergenic 30.0%
AAATTATCTTTACGTGTCAG+AGG - Chr4:72858480-72858499 MsG0480022465.01.T01:CDS 30.0%
AACCGTTTATTTCAATCCAA+TGG - Chr4:72858352-72858371 MsG0480022465.01.T01:intron 30.0%
AATTTCAGAGTATGTCTACA+AGG - Chr4:72856938-72856957 MsG0480022465.01.T01:intron 30.0%
AGAAAACAAAGAAAGAGACT+GGG - Chr4:72858258-72858277 MsG0480022465.01.T01:intron 30.0%
AGAAACATGGTTATATCACA+TGG + Chr4:72857054-72857073 None:intergenic 30.0%
AGAGGACATTTATAATGAGA+GGG + Chr4:72857597-72857578 None:intergenic 30.0%
ATGATTACTCGATCATTCAT+TGG + Chr4:72858527-72858546 None:intergenic 30.0%
ATTACTCAATAATCCATTGG+AGG + Chr4:72858632-72858651 None:intergenic 30.0%
ATTTACTCAACTCAAAGTTG+TGG - Chr4:72859046-72859065 MsG0480022465.01.T01:CDS 30.0%
CAAAGGAATAAAAATGTTGG+TGG + Chr4:72857836-72857855 None:intergenic 30.0%
CAATAGTTCGAATTGTTCAA+AGG - Chr4:72856604-72856623 MsG0480022465.01.T01:intron 30.0%
CAGATTCACAAAACAATACT+GGG + Chr4:72856989-72857008 None:intergenic 30.0%
CATTTATTCAATTCGCATGA+AGG - Chr4:72856108-72856127 MsG0480022465.01.T01:CDS 30.0%
CTTCAATTATTTATGCATGG+GGG - Chr4:72857015-72857034 MsG0480022465.01.T01:intron 30.0%
GACCATTGGATTGAAATAAA+CGG + Chr4:72858357-72858376 None:intergenic 30.0%
GAGCTTCAATTATTTATGCA+TGG - Chr4:72857012-72857031 MsG0480022465.01.T01:intron 30.0%
GCTTCAATTATTTATGCATG+GGG - Chr4:72857014-72857033 MsG0480022465.01.T01:intron 30.0%
GTTACAAATATGCACTCAAA+AGG - Chr4:72856197-72856216 MsG0480022465.01.T01:CDS 30.0%
TAATCATGTCTAGGTAAATC+TGG - Chr4:72858540-72858559 MsG0480022465.01.T01:CDS 30.0%
TATTTAACCTTGGACTGTAT+TGG - Chr4:72858982-72859001 MsG0480022465.01.T01:CDS 30.0%
TCAGATTCACAAAACAATAC+TGG + Chr4:72856990-72857009 None:intergenic 30.0%
! AACCTTTTTCTGTTCCAAAT+CGG + Chr4:72858167-72858186 None:intergenic 30.0%
! AGTATACTCGAAGTAGTTTT+TGG + Chr4:72856667-72856686 None:intergenic 30.0%
! ATGTGCAGAGTATTAATTTG+AGG + Chr4:72857332-72857351 None:intergenic 30.0%
! ATTCTCGGTACTAATTTTGA+TGG - Chr4:72858826-72858845 MsG0480022465.01.T01:CDS 30.0%
! GGTGGAAACAAATATCTTTT+AGG - Chr4:72858071-72858090 MsG0480022465.01.T01:intron 30.0%
! GTATACTCGAAGTAGTTTTT+GGG + Chr4:72856666-72856685 None:intergenic 30.0%
! TGTGCAGAGTATTAATTTGA+GGG + Chr4:72857331-72857350 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG - Chr4:72857466-72857485 MsG0480022465.01.T01:intron 30.0%
!! AGTATTGTTTTGTGAATCTG+AGG - Chr4:72856989-72857008 MsG0480022465.01.T01:intron 30.0%
!! GTATTGTTTTGTGAATCTGA+GGG - Chr4:72856990-72857009 MsG0480022465.01.T01:intron 30.0%
!! TTACCTCAGTTAATTGGTTT+TGG + Chr4:72855882-72855901 None:intergenic 30.0%
!!! GTGGTGTTGACTTAAAATTT+TGG - Chr4:72858779-72858798 MsG0480022465.01.T01:CDS 30.0%
!!! GTTTAGCTTTACTTTTTTGG+TGG + Chr4:72857998-72858017 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + Chr4:72857465-72857484 None:intergenic 30.0%
AAATTATCTTTAGGCGTCAG+AGG - Chr4:72858588-72858607 MsG0480022465.01.T01:CDS 35.0%
AAGTAAAGCTTCGATTCCAA+TGG + Chr4:72856169-72856188 None:intergenic 35.0%
AAGTGAACGAGAGATAAAAG+AGG + Chr4:72857869-72857888 None:intergenic 35.0%
AAGTTACATGTCAATCCCAT+TGG - Chr4:72856150-72856169 MsG0480022465.01.T01:CDS 35.0%
ACGATACGATCAACAACATA+TGG - Chr4:72856071-72856090 MsG0480022465.01.T01:CDS 35.0%
AGCAATAAATTTGGAGAGGT+TGG - Chr4:72859130-72859149 MsG0480022465.01.T01:CDS 35.0%
AGTAAAGCTTCGATTCCAAT+GGG + Chr4:72856168-72856187 None:intergenic 35.0%
ATGAACAATGTCACCAATGA+TGG + Chr4:72857087-72857106 None:intergenic 35.0%
ATGAATTGCTCAGGAGATAA+TGG + Chr4:72858910-72858929 None:intergenic 35.0%
ATGATCGAGTAATCATGTCT+AGG - Chr4:72858531-72858550 MsG0480022465.01.T01:CDS 35.0%
ATTACTCGATCATTCATTGG+AGG + Chr4:72858524-72858543 None:intergenic 35.0%
CAAACATATCTTCAGTGATG+TGG + Chr4:72856392-72856411 None:intergenic 35.0%
CACATCACTGAAGATATGTT+TGG - Chr4:72856390-72856409 MsG0480022465.01.T01:intron 35.0%
CACTACACCTGTATTTATTG+AGG - Chr4:72856734-72856753 MsG0480022465.01.T01:intron 35.0%
CTAAATTCAGTTGTGAAACC+AGG + Chr4:72856255-72856274 None:intergenic 35.0%
CTCAATAAATACAGGTGTAG+TGG + Chr4:72856736-72856755 None:intergenic 35.0%
CTCCTATGTACAAAAGATCA+TGG - Chr4:72856007-72856026 MsG0480022465.01.T01:CDS 35.0%
CTCTCAAGTTCGATTATTCT+CGG - Chr4:72858811-72858830 MsG0480022465.01.T01:CDS 35.0%
GAGAAAACAAAGAAAGAGAC+TGG - Chr4:72858257-72858276 MsG0480022465.01.T01:intron 35.0%
GAGAGGACATTTATAATGAG+AGG - Chr4:72857577-72857596 MsG0480022465.01.T01:intron 35.0%
GAGAGGACATTTATAATGAG+AGG + Chr4:72857596-72857577 None:intergenic 35.0%
GTTGTTGATCGTATCGTAAA+TGG + Chr4:72856068-72856087 None:intergenic 35.0%
TATGGGATGTATCAAATGAG+AGG + Chr4:72858415-72858434 None:intergenic 35.0%
TCAAAGGACACAATTGTATG+AGG - Chr4:72856620-72856639 MsG0480022465.01.T01:intron 35.0%
TCAATAATCCATTGGAGGTT+TGG + Chr4:72858627-72858646 None:intergenic 35.0%
TCGAAGCTTTACTTAAGAGA+TGG - Chr4:72856175-72856194 MsG0480022465.01.T01:CDS 35.0%
TGTACACTAATTACACATCC+AGG - Chr4:72857801-72857820 MsG0480022465.01.T01:intron 35.0%
TTACATTATGACCGTTGGAT+TGG - Chr4:72857608-72857627 MsG0480022465.01.T01:intron 35.0%
TTTCAATCTCTTTCAGATCC+CGG + Chr4:72856539-72856558 None:intergenic 35.0%
! CACCATGATCTTTTGTACAT+AGG + Chr4:72856012-72856031 None:intergenic 35.0%
! GTAATTATGTCTAGGACTCT+AGG - Chr4:72858647-72858666 MsG0480022465.01.T01:CDS 35.0%
! TAGCTTAAGCTTGGTAACAA+TGG - Chr4:72858050-72858069 MsG0480022465.01.T01:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + Chr4:72857231-72857250 None:intergenic 35.0%
!! AGACATACTCTGAAATTGCA+AGG + Chr4:72856935-72856954 None:intergenic 35.0%
!!! CTTCTTTTTTGAACTTGCAG+AGG + Chr4:72859181-72859200 None:intergenic 35.0%
AAAAGGAGAGAGAGAGACAA+TGG + Chr4:72857729-72857748 None:intergenic 40.0%
AAAGGAGAGAGAGAGACAAT+GGG + Chr4:72857728-72857747 None:intergenic 40.0%
ACAATGGTTGAAAACCCACT+TGG - Chr4:72858747-72858766 MsG0480022465.01.T01:CDS 40.0%
ACACACACACACACACATAT+AGG - Chr4:72857542-72857561 MsG0480022465.01.T01:intron 40.0%
ACACACACACACACATATAG+GGG - Chr4:72857544-72857563 MsG0480022465.01.T01:intron 40.0%
ACATATCTTCAGTGATGTGG+AGG + Chr4:72856389-72856408 None:intergenic 40.0%
ATGGTTTGAATGATATCCCC+AGG + Chr4:72855937-72855956 None:intergenic 40.0%
ATGTCTACAAGGATTCGTGA+CGG - Chr4:72856949-72856968 MsG0480022465.01.T01:intron 40.0%
ATTCAGTTGTGAAACCAGGT+CGG + Chr4:72856251-72856270 None:intergenic 40.0%
CAATGGTTGAAAACCCACTT+GGG - Chr4:72858748-72858767 MsG0480022465.01.T01:CDS 40.0%
CACACACACACACACATATA+GGG - Chr4:72857543-72857562 MsG0480022465.01.T01:intron 40.0%
CGAGAATAATCGAACTTGAG+AGG + Chr4:72858813-72858832 None:intergenic 40.0%
CTACTTCGAGTATACTAAGC+TGG - Chr4:72856671-72856690 MsG0480022465.01.T01:intron 40.0%
GAGCATTACATTATGACCGT+TGG - Chr4:72857603-72857622 MsG0480022465.01.T01:intron 40.0%
GAGCATTACTTATGGACCAT+TGG + Chr4:72858371-72858390 None:intergenic 40.0%
GTTGGAGTTAATTCCACCAA+AGG - Chr4:72859148-72859167 MsG0480022465.01.T01:CDS 40.0%
TATATAGCCAGCATCTTGGA+TGG + Chr4:72859307-72859326 None:intergenic 40.0%
TCTGTATATAGCCAGCATCT+TGG + Chr4:72859311-72859330 None:intergenic 40.0%
TGCAAAAATTGCAAGCCACT+TGG + Chr4:72856348-72856367 None:intergenic 40.0%
! AATTGAGAATCGCTTGTTGC+CGG - Chr4:72856517-72856536 MsG0480022465.01.T01:intron 40.0%
! ATAGGGGTGTATCAAATGAG+AGG - Chr4:72857560-72857579 MsG0480022465.01.T01:intron 40.0%
! ATCATCGGGAGAATCAGTTT+TGG + Chr4:72855911-72855930 None:intergenic 40.0%
! CTTAAGCTTGGTAACAATGG+TGG - Chr4:72858053-72858072 MsG0480022465.01.T01:intron 40.0%
! TAGCTGCAATTGTCAAGTTG+AGG + Chr4:72859023-72859042 None:intergenic 40.0%
! TCTAGGACTCTAGGTAAATC+TGG - Chr4:72858656-72858675 MsG0480022465.01.T01:CDS 40.0%
!! TACTCGAAGTAGTTTTTGGG+AGG + Chr4:72856663-72856682 None:intergenic 40.0%
!! TTCTTGGAGAGCATCAACAA+GGG - Chr4:72859229-72859248 MsG0480022465.01.T01:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - Chr4:72857470-72857489 MsG0480022465.01.T01:intron 40.0%
!!! GACACTCATTTTGAAACGGA+TGG + Chr4:72857489-72857470 None:intergenic 40.0%
ATATCCCCAGGAAGATCATC+GGG + Chr4:72855925-72855944 None:intergenic 45.0%
ATGTGCGATCCAGTTACCAA+AGG - Chr4:72856320-72856339 MsG0480022465.01.T01:intron 45.0%
ATTCTCCCGATGATCTTCCT+GGG - Chr4:72855917-72855936 MsG0480022465.01.T01:CDS 45.0%
CAGTTACCAAAGGTTCCAAG+TGG - Chr4:72856330-72856349 MsG0480022465.01.T01:intron 45.0%
GATATCCCCAGGAAGATCAT+CGG + Chr4:72855926-72855945 None:intergenic 45.0%
GCATTTGCCAATACAGTCCA+AGG + Chr4:72858992-72859011 None:intergenic 45.0%
GCGTGGAAAGAAGGAGAAAA+GGG - Chr4:72858297-72858316 MsG0480022465.01.T01:intron 45.0%
TACAGGTGTAGTGGTGAGAA+TGG + Chr4:72856727-72856746 None:intergenic 45.0%
TCGATCATTCATTGGAGGCT+TGG + Chr4:72858519-72858538 None:intergenic 45.0%
TTGCAGAGGCGATAAACCTT+TGG + Chr4:72859167-72859186 None:intergenic 45.0%
! ATTGAGAATCGCTTGTTGCC+GGG - Chr4:72856518-72856537 MsG0480022465.01.T01:intron 45.0%
! CACTTGGAACCTTTGGTAAC+TGG + Chr4:72856332-72856351 None:intergenic 45.0%
! GATTCGTGACGGAGTATTTC+GGG - Chr4:72856960-72856979 MsG0480022465.01.T01:intron 45.0%
! GGATTCGTGACGGAGTATTT+CGG - Chr4:72856959-72856978 MsG0480022465.01.T01:intron 45.0%
! TGTTGGTGGAGTCTTTATCC+TGG + Chr4:72857822-72857841 None:intergenic 45.0%
!! AAAACCCACTTGGGTTGGTT+CGG - Chr4:72858757-72858776 MsG0480022465.01.T01:CDS 45.0%
!! GAGGGTGAGAGCATTACTTA+TGG + Chr4:72858379-72858398 None:intergenic 45.0%
!! GGCCGATTTGGAACAGAAAA+AGG - Chr4:72858162-72858181 MsG0480022465.01.T01:intron 45.0%
!! GTGTTAGCTTTAGGCCGATT+TGG - Chr4:72858150-72858169 MsG0480022465.01.T01:intron 45.0%
!! GTTCTTGGAGAGCATCAACA+AGG - Chr4:72859228-72859247 MsG0480022465.01.T01:CDS 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - Chr4:72857469-72857488 MsG0480022465.01.T01:intron 45.0%
!! AATAAAATAAATTAAAACAA+TGG - Chr4:72858731-72858750 MsG0480022465.01.T01:CDS 5.0%
AGACAATGGGAACTGGTGAG+TGG + Chr4:72857715-72857734 None:intergenic 50.0%
AGCCAGCATCTTGGATGGAT+TGG + Chr4:72859302-72859321 None:intergenic 50.0%
ATTGGAGGCTTGGATGTCAG+AGG + Chr4:72858509-72858528 None:intergenic 50.0%
ATTGGAGGTTTGGACGTCAG+AGG + Chr4:72858617-72858636 None:intergenic 50.0%
CAGAGGCGATAAACCTTTGG+TGG + Chr4:72859164-72859183 None:intergenic 50.0%
CGCGTGGAAAGAAGGAGAAA+AGG - Chr4:72858296-72858315 MsG0480022465.01.T01:intron 50.0%
GAGAGAGAGACAATGGGAAC+TGG + Chr4:72857722-72857741 None:intergenic 50.0%
GAGTTGCATCTTTACCGACC+TGG - Chr4:72856234-72856253 MsG0480022465.01.T01:intron 50.0%
GATTCTCCCGATGATCTTCC+TGG - Chr4:72855916-72855935 MsG0480022465.01.T01:CDS 50.0%
GGTTGAAAACCCACTTGGGT+TGG - Chr4:72858752-72858771 MsG0480022465.01.T01:CDS 50.0%
GTCCAATCCATCCAAGATGC+TGG - Chr4:72859297-72859316 MsG0480022465.01.T01:CDS 50.0%
TCTGACGTCCAAACCTCCAA+TGG - Chr4:72858616-72858635 MsG0480022465.01.T01:CDS 50.0%
TGCAAGCCACTTGGAACCTT+TGG + Chr4:72856339-72856358 None:intergenic 50.0%
TTCTCCCGATGATCTTCCTG+GGG - Chr4:72855918-72855937 MsG0480022465.01.T01:CDS 50.0%
! GAAGAAAGCGCGTGGAAAGA+AGG - Chr4:72858288-72858307 MsG0480022465.01.T01:intron 50.0%
! GTTGAGAAGAAGAAAGCGCG+TGG - Chr4:72858280-72858299 MsG0480022465.01.T01:intron 50.0%
!! GCATCTTGGATGGATTGGAC+TGG + Chr4:72859297-72859316 None:intergenic 50.0%
ACCACCGAACCAACCCAAGT+GGG + Chr4:72858764-72858783 None:intergenic 55.0%
GAGAAAGTTTCACACGCGCG+TGG + Chr4:72857682-72857701 None:intergenic 55.0%
CACCACCGAACCAACCCAAG+TGG + Chr4:72858765-72858784 None:intergenic 60.0%
!! ACCCACTTGGGTTGGTTCGG+TGG - Chr4:72858760-72858779 MsG0480022465.01.T01:CDS 60.0%
!! GAGAGCATCAACAAGGGCCG+TGG - Chr4:72859235-72859254 MsG0480022465.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 72855862 72859340 72855862 ID=MsG0480022465.01;Name=MsG0480022465.01
Chr4 mRNA 72855862 72859340 72855862 ID=MsG0480022465.01.T01;Parent=MsG0480022465.01;Name=MsG0480022465.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|421
Chr4 exon 72858447 72859340 72858447 ID=MsG0480022465.01.T01:exon:10199;Parent=MsG0480022465.01.T01
Chr4 exon 72855862 72856233 72855862 ID=MsG0480022465.01.T01:exon:10198;Parent=MsG0480022465.01.T01
Chr4 CDS 72858447 72859340 72858447 ID=MsG0480022465.01.T01:cds;Parent=MsG0480022465.01.T01
Chr4 CDS 72855862 72856233 72855862 ID=MsG0480022465.01.T01:cds;Parent=MsG0480022465.01.T01
Gene Sequence

>MsG0480022465.01.T01

ATGACAAGTTTTAAAAACCAAAACCAATTAACTGAGGTAAATAAAACCAAAACTGATTCTCCCGATGATCTTCCTGGGGATATCATTCAAACCATTTTCTCTTTATTACCAATAAAAGATGCAATTGTAACAACATCAACTGTATCTCCTATGTACAAAAGATCATGGTGTCATAATCGTCAATTTTTGTTTGGTAGAGACTTCCATTTACGATACGATCAACAACATATGGCTGCAATTGTTGATCATTTATTCAATTCGCATGAAGGTAACAAAATTAAAACATTTAAGTTACATGTCAATCCCATTGGAATCGAAGCTTTACTTAAGAGATGGTTACAAATATGCACTCAAAAGGATCTAGAAGATCTCGAGTTGCATCTTTACCGACCTGGTTTCACAACTGAATTTAGTGTTTTTAACGCACTACACAAGTTAAAAACACTAAAATTGGTTAAATGTGCGATCCAGTTACCAAAGGTTCCAAGTGGCTTGCAATTTTTGCAAACACTAACTCTGTGTAACCTCCACATCACTGAAGATATGTTTGGTGTGTTGATTGAACATTGTAAAATGCTTAAAACTGTTGATCTGATTAAATGCTATACCATTAAGAAATTGAAATTAATTGTTAGAGAAAATAAACATTTCAAAAAATTGAGAATCGCTTGTTGCCGGGATCTGAAAGAGATTGAAATTGATTCACCAACTCTTCGTTCAATTTTTTATCATGGAAAATTTTCAATAGTTCGAATTGTTCAAAGGACACAATTGTATGAGGCATTCTTTCACTTTATACCTCCCAAAAACTACTTCGAGTATACTAAGCTGGAAGCGTTAGTCAAAGATCTCTCTCATGTCTCCATTCTCACCACTACACCTGTATTTATTGAGCTAAATGATTATATATTTAACCTTGGACTGTATTGGCAAATGCACGATAAACCTCAACTTGACAATTGCAGCTATAAATTTACTCAACTCAAAGTTGTGGTGCTGAGAAGTTTTAAGTATTTGTCTTCTGAACTTGAGTTAGTAAAGATACTTTTGCAAAGAGCAATAAATTTGGAGAGGTTGGAGTTAATTCCACCAAAGGTTTATCGCCTCTGCAAGTTCAAAAAAGAAGATACACCAACATACGAAAATCTTTTTTGTTCTTGGAGAGCATCAACAAGGGCCGTGGTAAAGTTACATAAATATCATGTTAAGAAGAGTTTCGCCAGTCCAATCCATCCAAGATGCTGGCTATATACAGATGCATGTTGA
Protein sequence

>MsG0480022465.01.T01

MTSFKNQNQLTEVNKTKTDSPDDLPGDIIQTIFSLLPIKDAIVTTSTVSPMYKRSWCHNRQFLFGRDFHLRYDQQHMAAIVDHLFNSHEGNKIKTFKLHVNPIGIEALLKRWLQICTQKDLEDLELHLYRPGFTTEFSVFNALHKLKTLKLVKCAIQLPKVPSGLQFLQTLTLCNLHITEDMFGVLIEHCKMLKTVDLIKCYTIKKLKLIVRENKHFKKLRIACCRDLKEIEIDSPTLRSIFYHGKFSIVRIVQRTQLYEAFFHFIPPKNYFEYTKLEALVKDLSHVSILTTTPVFIELNDYIFNLGLYWQMHDKPQLDNCSYKFTQLKVVVLRSFKYLSSELELVKILLQRAINLERLELIPPKVYRLCKFKKEDTPTYENLFCSWRASTRAVVKLHKYHVKKSFASPIHPRCWLYTDAC*