AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026641.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026641.01.T01 MTR_0009s0080 79.805 307 62 0 19 325 1 307 5.40e-175 492
MsG0580026641.01.T01 MTR_5g007410 52.469 324 144 6 11 330 4 321 3.78e-102 308
MsG0580026641.01.T01 MTR_6g045333 50.323 310 148 4 18 324 45 351 2.97e-88 272
MsG0580026641.01.T01 MTR_5g045170 49.180 305 141 6 32 331 10 305 2.42e-80 250
MsG0580026641.01.T01 MTR_5g045510 51.079 278 129 5 37 313 13 284 7.41e-78 243
MsG0580026641.01.T01 MTR_3g071850 50.853 293 136 6 37 324 25 314 2.30e-75 248
MsG0580026641.01.T01 MTR_2g036470 46.626 326 146 9 23 335 3 313 3.19e-73 232
MsG0580026641.01.T01 MTR_5g039450 44.186 301 135 6 32 324 2 277 2.72e-66 213
MsG0580026641.01.T01 MTR_5g039470 45.878 279 138 5 52 324 1 272 5.47e-66 211
MsG0580026641.01.T01 MTR_5g039490 47.170 265 129 4 37 295 21 280 3.31e-64 208
MsG0580026641.01.T01 MTR_5g073420 44.816 299 142 14 38 331 13 293 2.84e-62 203
MsG0580026641.01.T01 MTR_1g066830 42.414 290 162 4 38 325 10 296 1.06e-60 199
MsG0580026641.01.T01 MTR_5g058100 42.424 297 160 7 38 331 11 299 2.53e-59 196
MsG0580026641.01.T01 MTR_5g058000 41.806 299 151 8 38 331 10 290 1.02e-57 191
MsG0580026641.01.T01 MTR_5g061410 42.808 292 131 7 38 325 7 266 4.61e-57 186
MsG0580026641.01.T01 MTR_1g085120 40.956 293 162 4 38 325 181 467 1.66e-55 188
MsG0580026641.01.T01 MTR_1g085120 40.956 293 162 4 38 325 94 380 1.92e-55 187
MsG0580026641.01.T01 MTR_6g022500 39.116 294 170 5 38 329 103 389 3.73e-55 187
MsG0580026641.01.T01 MTR_1g085120 40.956 293 162 4 38 325 169 455 9.22e-55 187
MsG0580026641.01.T01 MTR_5g031970 39.735 302 135 7 32 329 12 270 2.01e-51 174
MsG0580026641.01.T01 MTR_1089s0010 38.014 292 170 4 38 329 10 290 4.90e-51 174
MsG0580026641.01.T01 MTR_7g026500 37.700 313 160 9 38 330 16 313 9.89e-51 174
MsG0580026641.01.T01 MTR_7g055943 35.873 315 185 4 20 325 18 324 3.52e-50 173
MsG0580026641.01.T01 MTR_5g063950 41.522 289 134 7 38 324 10 265 6.19e-50 170
MsG0580026641.01.T01 MTR_6g011460 38.356 292 170 5 38 328 48 330 1.42e-49 171
MsG0580026641.01.T01 MTR_1g108820 39.933 298 157 8 38 324 10 296 1.48e-49 169
MsG0580026641.01.T01 MTR_1g108820 39.933 298 157 8 38 324 10 296 4.82e-49 169
MsG0580026641.01.T01 MTR_1g059860 41.603 262 115 6 98 324 1 259 5.48e-49 167
MsG0580026641.01.T01 MTR_6g040270 37.000 300 172 6 29 324 1 287 7.93e-48 165
MsG0580026641.01.T01 MTR_4g039440 37.500 296 164 7 38 325 36 318 1.32e-47 166
MsG0580026641.01.T01 MTR_5g061680 39.456 294 135 10 38 325 13 269 1.28e-44 155
MsG0580026641.01.T01 MTR_6g022360 39.041 292 153 6 38 325 45 315 5.78e-44 156
MsG0580026641.01.T01 MTR_1g108800 39.446 289 114 9 38 317 10 246 1.55e-40 145
MsG0580026641.01.T01 MTR_3g095600 39.130 230 114 4 38 264 6 212 3.54e-38 138
MsG0580026641.01.T01 MTR_5g073340 35.690 297 153 9 38 329 10 273 3.23e-37 135
MsG0580026641.01.T01 MTR_7g056433 36.700 297 165 11 38 325 14 296 1.03e-36 136
MsG0580026641.01.T01 MTR_6g044572 34.891 321 148 11 38 327 4 294 1.30e-36 136
MsG0580026641.01.T01 MTR_6g044560 34.891 321 148 11 38 327 180 470 1.07e-35 136
MsG0580026641.01.T01 MTR_5g065100 42.081 221 118 4 34 252 6 218 4.04e-35 133
MsG0580026641.01.T01 MTR_5g073370 35.000 300 164 11 38 325 7 287 7.99e-35 131
MsG0580026641.01.T01 MTR_3g071790 49.020 153 74 3 179 329 1 151 8.94e-33 121
MsG0580026641.01.T01 MTR_7g098510 32.803 314 180 8 23 325 5 298 5.77e-29 115
MsG0580026641.01.T01 MTR_3g107100 33.557 298 172 10 38 325 13 294 6.76e-29 115
MsG0580026641.01.T01 MTR_1g041245 33.333 300 165 15 38 324 13 290 5.91e-28 112
MsG0580026641.01.T01 MTR_7g115270 34.853 307 168 14 38 324 14 308 6.74e-28 112
MsG0580026641.01.T01 MTR_2g007400 31.579 304 177 9 38 324 14 303 1.28e-27 112
MsG0580026641.01.T01 MTR_5g076150 35.533 197 109 6 145 325 11 205 7.15e-27 106
MsG0580026641.01.T01 MTR_3g095640 41.317 167 90 6 28 187 10 175 1.02e-25 101
MsG0580026641.01.T01 MTR_7g098480 33.333 315 175 10 23 325 5 296 8.32e-25 104
MsG0580026641.01.T01 MTR_3g107120 32.581 310 167 11 38 324 11 301 1.29e-24 103
MsG0580026641.01.T01 MTR_2g007450 31.579 323 184 9 38 329 18 334 4.09e-24 102
MsG0580026641.01.T01 MTR_7g032380 30.717 293 148 13 41 324 16 262 5.81e-23 98.2
MsG0580026641.01.T01 MTR_7g056447 37.017 181 106 5 38 216 11 185 8.17e-23 96.7
MsG0580026641.01.T01 MTR_3g108560 33.981 309 149 15 38 325 22 296 8.81e-23 97.1
MsG0580026641.01.T01 MTR_7g098570 30.938 320 189 8 18 325 1 300 1.55e-22 98.2
MsG0580026641.01.T01 MTR_3g108560 33.981 309 149 15 38 325 22 296 2.49e-22 97.1
MsG0580026641.01.T01 MTR_0492s0020 32.323 297 165 11 38 325 88 357 3.42e-22 97.1
MsG0580026641.01.T01 MTR_3g107160 29.373 303 177 10 38 324 12 293 3.47e-22 96.7
MsG0580026641.01.T01 MTR_0543s0010 32.323 297 165 11 38 325 88 357 3.62e-22 97.1
MsG0580026641.01.T01 MTR_2g007500 28.797 316 182 10 25 324 2 290 4.74e-22 95.5
MsG0580026641.01.T01 MTR_1g107385 30.945 307 178 9 38 324 34 326 4.97e-22 96.3
MsG0580026641.01.T01 MTR_7g033915 31.046 306 154 14 38 327 13 277 5.11e-22 95.9
MsG0580026641.01.T01 MTR_3g040700 31.353 303 160 14 38 325 45 314 6.61e-22 96.3
MsG0580026641.01.T01 MTR_6g022520 33.333 231 138 6 102 325 1 222 7.16e-22 94.7
MsG0580026641.01.T01 MTR_5g058350 35.052 194 98 2 29 222 1 166 8.42e-22 91.3
MsG0580026641.01.T01 MTR_7g075820 31.090 312 173 13 38 324 8 302 1.30e-21 95.1
MsG0580026641.01.T01 MTR_3g036250 33.553 304 152 15 38 325 7 276 1.53e-21 94.4
MsG0580026641.01.T01 MTR_5g008950 28.283 297 179 10 38 325 1 272 2.96e-21 93.6
MsG0580026641.01.T01 MTR_2g005990 30.065 306 177 10 38 324 8 295 4.00e-21 93.6
MsG0580026641.01.T01 MTR_3g099830 29.630 297 162 11 38 324 14 273 5.97e-21 92.8
MsG0580026641.01.T01 MTR_5g083890 30.323 310 179 10 38 324 8 303 7.40e-21 93.6
MsG0580026641.01.T01 MTR_3g023630 32.653 294 164 11 38 320 10 280 9.61e-21 92.4
MsG0580026641.01.T01 MTR_7g007080 29.530 298 182 10 38 324 6 286 1.54e-20 91.7
MsG0580026641.01.T01 MTR_5g008920 31.399 293 167 16 43 324 19 288 1.79e-20 91.7
MsG0580026641.01.T01 MTR_7g088260 30.887 327 187 15 17 330 75 375 1.98e-20 92.0
MsG0580026641.01.T01 MTR_7g056287 31.313 297 176 10 38 326 5 281 2.07e-20 91.3
MsG0580026641.01.T01 MTR_4g098590 31.788 302 174 11 40 324 3 289 3.36e-20 90.5
MsG0580026641.01.T01 MTR_7g056277 31.438 299 165 10 38 322 261 533 4.45e-20 91.7
MsG0580026641.01.T01 MTR_5g054920 40.164 122 67 2 204 325 2 117 1.03e-19 85.9
MsG0580026641.01.T01 MTR_1g053025 30.233 301 189 10 38 324 4 297 1.32e-19 89.0
MsG0580026641.01.T01 MTR_6g022390 42.188 128 66 3 38 158 7 133 3.49e-18 80.5
MsG0580026641.01.T01 MTR_5g024050 50.538 93 43 2 179 269 1 92 3.80e-18 79.3
MsG0580026641.01.T01 MTR_7g033990 31.803 305 171 13 38 327 13 295 4.98e-18 84.7
MsG0580026641.01.T01 MTR_7g007340 30.508 295 144 11 38 322 15 258 7.15e-18 84.0
MsG0580026641.01.T01 MTR_7g007370 30.479 292 137 10 38 324 19 249 1.18e-17 83.2
MsG0580026641.01.T01 MTR_3g109982 38.947 190 94 6 38 219 15 190 1.51e-17 84.0
MsG0580026641.01.T01 MTR_3g110010 38.947 190 94 6 38 219 15 190 2.30e-17 83.6
MsG0580026641.01.T01 MTR_3g105060 28.912 294 182 9 38 325 16 288 3.44e-17 82.0
MsG0580026641.01.T01 MTR_3g108150 30.508 295 180 11 38 324 15 292 6.23e-17 81.3
MsG0580026641.01.T01 MTR_2g008310 28.667 300 175 9 38 322 4 279 1.36e-16 80.5
MsG0580026641.01.T01 MTR_6g083130 51.685 89 30 2 132 219 232 308 1.81e-16 79.3
MsG0580026641.01.T01 MTR_7g034115 29.667 300 175 12 38 324 13 289 1.88e-16 79.7
MsG0580026641.01.T01 MTR_3g026920 28.354 328 164 11 38 324 21 318 3.41e-16 79.3
MsG0580026641.01.T01 MTR_3g467400 30.769 247 148 9 38 266 5 246 3.65e-16 79.0
MsG0580026641.01.T01 MTR_5g045700 58.333 60 21 2 26 85 6 61 1.35e-15 70.9
MsG0580026641.01.T01 MTR_3g082890 28.613 346 185 15 16 324 71 391 1.49e-15 77.8
MsG0580026641.01.T01 MTR_3g087260 30.675 326 161 14 25 335 1 276 2.17e-15 77.0
MsG0580026641.01.T01 MTR_3g087260 34.211 190 106 6 142 324 184 361 2.93e-12 67.8
MsG0580026641.01.T01 MTR_5g073400 44.643 112 53 4 110 218 5 110 2.22e-15 72.4
MsG0580026641.01.T01 MTR_3g082890 28.704 324 175 13 32 324 13 311 2.36e-15 77.0
MsG0580026641.01.T01 MTR_7g032370 29.066 289 155 16 51 324 5 258 1.51e-14 73.9
MsG0580026641.01.T01 MTR_0571s0010 48.750 80 40 1 38 117 34 112 2.14e-14 68.9
MsG0580026641.01.T01 MTR_7g060470 28.710 310 175 10 38 320 5 295 2.75e-14 73.6
MsG0580026641.01.T01 MTR_8g085430 33.503 197 100 11 41 219 16 199 4.11e-14 72.4
MsG0580026641.01.T01 MTR_7g056430 29.966 297 159 14 38 324 11 268 8.04e-14 73.2
MsG0580026641.01.T01 MTR_2g008320 27.517 298 185 9 38 322 54 333 8.34e-14 72.4
MsG0580026641.01.T01 MTR_7g056420 29.966 297 159 14 38 324 11 268 9.27e-14 72.8
MsG0580026641.01.T01 MTR_7g056020 29.195 298 165 12 38 324 18 280 3.43e-13 70.9
MsG0580026641.01.T01 MTR_7g114710 28.763 299 159 14 38 325 15 270 4.09e-13 69.7
MsG0580026641.01.T01 MTR_8g062310 31.122 196 119 7 38 229 27 210 7.89e-13 69.3
MsG0580026641.01.T01 MTR_7g034110 27.483 302 142 12 38 324 13 252 1.70e-12 67.8
MsG0580026641.01.T01 MTR_3g095630 39.535 86 44 2 240 325 18 95 2.54e-12 65.1
MsG0580026641.01.T01 MTR_4g134150 32.184 174 106 6 49 219 23 187 4.82e-12 65.1
MsG0580026641.01.T01 MTR_5g024040 50.847 59 29 0 17 75 3 61 1.01e-11 60.1
MsG0580026641.01.T01 MTR_3g114200 33.333 138 82 4 38 171 21 152 4.06e-11 61.2
MsG0580026641.01.T01 MTR_7g022105 26.471 306 183 7 38 325 51 332 4.15e-11 63.9
MsG0580026641.01.T01 MTR_4g032730 25.575 348 186 12 38 324 17 352 4.29e-11 64.3
MsG0580026641.01.T01 MTR_7g021990 27.063 303 182 9 38 325 50 328 4.46e-11 63.9
MsG0580026641.01.T01 MTR_7g033945 32.353 170 97 8 38 197 13 174 6.21e-11 61.2
MsG0580026641.01.T01 MTR_5g012840 28.626 262 140 14 61 308 14 242 7.43e-11 62.8
MsG0580026641.01.T01 MTR_7g021950 27.063 303 182 9 38 325 50 328 8.75e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026641.01.T01 AT3G59240 27.606 355 179 15 38 325 10 353 1.26e-14 75.1
MsG0580026641.01.T01 AT4G10400 31.183 186 116 7 38 218 4 182 2.12e-14 73.9
MsG0580026641.01.T01 AT4G10400 31.183 186 116 7 38 218 4 182 2.12e-14 73.9
MsG0580026641.01.T01 AT4G10400 31.183 186 116 7 38 218 4 182 2.12e-14 73.9
MsG0580026641.01.T01 AT1G06630 25.634 355 185 14 37 326 13 353 3.30e-14 73.2
MsG0580026641.01.T01 AT1G06630 25.634 355 185 14 37 326 13 353 3.30e-14 73.2
MsG0580026641.01.T01 AT5G38590 35.326 184 109 5 38 218 4 180 4.06e-14 72.0
MsG0580026641.01.T01 AT4G09920 31.183 186 116 7 38 218 4 182 4.66e-14 72.4
MsG0580026641.01.T01 AT4G09920 31.183 186 116 7 38 218 4 182 4.66e-14 72.4
MsG0580026641.01.T01 AT4G09920 31.183 186 116 7 38 218 4 182 5.20e-14 72.0
MsG0580026641.01.T01 AT4G09920 31.183 186 116 7 38 218 4 182 5.20e-14 72.0
MsG0580026641.01.T01 AT4G09920 31.183 186 116 7 38 218 4 182 5.20e-14 72.0
MsG0580026641.01.T01 AT3G56780 30.412 194 116 7 32 218 2 183 7.44e-14 72.4
MsG0580026641.01.T01 AT3G56780 30.412 194 116 7 32 218 2 183 8.71e-14 72.4
MsG0580026641.01.T01 AT3G56780 30.412 194 116 7 32 218 2 183 8.73e-14 72.4
MsG0580026641.01.T01 AT3G59200 34.211 190 111 7 38 218 9 193 1.05e-13 71.6
MsG0580026641.01.T01 AT4G14103 35.789 190 106 7 38 218 10 192 1.07e-13 72.0
MsG0580026641.01.T01 AT5G38590 35.326 184 109 5 38 218 4 180 1.18e-13 71.6
MsG0580026641.01.T01 AT3G59200 34.211 190 111 7 38 218 9 193 1.32e-13 72.0
MsG0580026641.01.T01 AT4G14103 35.789 190 106 7 38 218 10 192 1.46e-13 71.2
MsG0580026641.01.T01 AT4G14103 35.789 190 106 7 38 218 10 192 1.46e-13 71.2
MsG0580026641.01.T01 AT1G06630 25.348 359 189 14 32 325 17 361 1.61e-13 71.2
MsG0580026641.01.T01 AT5G41840 33.816 207 99 10 38 218 16 210 2.05e-13 71.2
MsG0580026641.01.T01 AT1G06630 25.424 354 185 14 37 325 13 352 2.06e-13 70.9
MsG0580026641.01.T01 AT1G06630 25.424 354 185 14 37 325 13 352 2.06e-13 70.9
MsG0580026641.01.T01 AT1G06630 25.424 354 185 14 37 325 13 352 2.06e-13 70.9
MsG0580026641.01.T01 AT1G06630 25.424 354 185 14 37 325 13 352 2.06e-13 70.9
MsG0580026641.01.T01 AT5G41830 28.108 370 167 19 38 322 15 370 3.20e-13 70.9
MsG0580026641.01.T01 AT1G06630 25.348 359 189 14 32 325 8 352 3.65e-13 70.5
MsG0580026641.01.T01 AT4G00160 33.838 198 116 6 28 218 8 197 5.24e-13 70.1
MsG0580026641.01.T01 AT2G26860 29.843 191 116 4 38 218 4 186 9.01e-12 66.2
MsG0580026641.01.T01 AT2G26860 29.843 191 116 4 38 218 4 186 9.01e-12 66.2
MsG0580026641.01.T01 AT5G56440 30.942 223 127 10 38 249 4 210 1.04e-11 65.9
MsG0580026641.01.T01 AT5G22670 31.282 195 116 8 30 218 5 187 1.15e-11 65.9
MsG0580026641.01.T01 AT4G00320 32.338 201 111 8 37 218 14 208 1.41e-11 65.9
MsG0580026641.01.T01 AT1G78750 27.805 205 135 5 23 218 5 205 1.55e-11 65.5
MsG0580026641.01.T01 AT1G78750 27.805 205 135 5 23 218 5 205 1.55e-11 65.5
MsG0580026641.01.T01 AT3G49150 33.158 190 112 6 38 218 18 201 1.64e-11 65.5
MsG0580026641.01.T01 AT3G42770 27.035 344 186 14 38 322 1 338 1.70e-11 65.5
MsG0580026641.01.T01 AT5G22720 29.787 188 116 5 38 218 25 203 2.10e-11 65.1
MsG0580026641.01.T01 AT4G26340 26.506 332 186 12 38 324 4 322 2.14e-11 65.1
MsG0580026641.01.T01 AT4G22280 25.806 341 189 10 38 326 4 332 2.40e-11 64.3
MsG0580026641.01.T01 AT5G56690 31.606 193 111 6 36 216 2 185 2.64e-11 64.7
MsG0580026641.01.T01 AT3G50710 32.461 191 110 6 38 218 4 185 4.15e-11 64.3
MsG0580026641.01.T01 AT3G26922 32.673 202 120 9 38 235 16 205 4.59e-11 63.5
MsG0580026641.01.T01 AT3G26922 32.673 202 120 9 38 235 16 205 4.59e-11 63.5
MsG0580026641.01.T01 AT4G00315 30.645 186 119 5 39 218 5 186 5.08e-11 63.9
MsG0580026641.01.T01 AT4G22280 26.453 344 189 11 38 329 4 335 5.21e-11 63.9
MsG0580026641.01.T01 AT5G56380 32.353 204 109 8 38 226 4 193 5.25e-11 63.9
MsG0580026641.01.T01 AT5G56380 32.353 204 109 8 38 226 4 193 5.25e-11 63.9

Find 49 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCAAACTCTTTCGATTA+AGG 0.181127 5:+41260052 MsG0580026641.01.T01:CDS
TTTGATGAGATTGGATAATA+AGG 0.225057 5:-41259718 None:intergenic
TTTGAAGTTTCGGACAATTC+TGG 0.256504 5:-41260037 None:intergenic
AAAACCCTTGATATGTGTTT+TGG 0.286635 5:+41259573 MsG0580026641.01.T01:CDS
ATCGAAAGAGTTTGAAGTTT+CGG 0.287818 5:-41260047 None:intergenic
AAAATTAGCTTTGATGAGAT+TGG 0.294902 5:-41259727 None:intergenic
ACATTATAAACTGCTCTAAA+AGG 0.304483 5:-41259765 None:intergenic
TCTCTCCGGCTGTCCCAAAT+TGG 0.341466 5:+41259629 MsG0580026641.01.T01:CDS
TAACGAAGTCTACACGAAAA+AGG 0.346588 5:+41259251 MsG0580026641.01.T01:CDS
AAGTGTTCTACAAACGAAAA+TGG 0.360911 5:-41259481 None:intergenic
TAGCTCTCCAATGATTAGAT+TGG 0.402800 5:-41259362 None:intergenic
AGGTTTATTTATCATTCCTA+TGG 0.406734 5:+41260072 MsG0580026641.01.T01:CDS
AGCCTCTATGTCTCGTTGTT+CGG 0.408220 5:+41259533 MsG0580026641.01.T01:CDS
TCGACTGCCAATCTAATCAT+TGG 0.435539 5:+41259355 MsG0580026641.01.T01:CDS
AAGAACCAAAACACATATCA+AGG 0.436415 5:-41259578 None:intergenic
TGATTGAACTTCGGTTATCC+TGG 0.438497 5:+41259970 MsG0580026641.01.T01:CDS
TCATGACTGGAGTGAGGTAC+TGG 0.438759 5:+41260007 MsG0580026641.01.T01:CDS
ATCCTCCAATTTGGGACAGC+CGG 0.450093 5:-41259634 None:intergenic
CTCCGGCTGTCCCAAATTGG+AGG 0.452689 5:+41259632 MsG0580026641.01.T01:CDS
TTGATGAGATTGGATAATAA+GGG 0.456611 5:-41259717 None:intergenic
TCAATTTCTCGTGCAGCCAT+CGG 0.503127 5:+41259053 MsG0580026641.01.T01:CDS
AAGAGAGAATGTAAATGAGT+AGG 0.505897 5:-41259137 None:intergenic
ATGAGTAACACGTGAAGTCC+AGG 0.514421 5:-41259988 None:intergenic
TTGAAAACTTGATTGAACTT+CGG 0.516260 5:+41259961 MsG0580026641.01.T01:CDS
GGTTCACTTTCGTAGAATTG+TGG 0.524320 5:+41259278 MsG0580026641.01.T01:CDS
GATTTCATGAAGCTTCTCTC+CGG 0.525661 5:+41259615 MsG0580026641.01.T01:CDS
AAACAGTGGGAACCATCGCT+TGG 0.526461 5:-41259199 None:intergenic
GACCGAACAACGAGACATAG+AGG 0.531527 5:-41259535 None:intergenic
GTTCTGAAAGCATATTTGAT+CGG 0.537910 5:-41259165 None:intergenic
GTTCTACAAACGAAAATGGT+AGG 0.543072 5:-41259477 None:intergenic
AGAAGCAAATCGACGTAGGG+TGG 0.545286 5:+41259416 MsG0580026641.01.T01:CDS
TTTCGTTTGTAGAACACTTG+TGG 0.547725 5:+41259485 MsG0580026641.01.T01:CDS
CTGGAAGACTGTTACCCAGT+TGG 0.550829 5:-41259674 None:intergenic
AGCAAATCGACGTAGGGTGG+AGG 0.553447 5:+41259419 MsG0580026641.01.T01:CDS
CTACCGTTCTTTCCAAGCGA+TGG 0.562465 5:+41259187 MsG0580026641.01.T01:CDS
CTTCACGTGTTACTCATGAC+TGG 0.578894 5:+41259994 MsG0580026641.01.T01:CDS
TCGAAGAAGCAAATCGACGT+AGG 0.588919 5:+41259412 MsG0580026641.01.T01:CDS
AACGAAAATGGTAGGCAACA+AGG 0.596542 5:-41259469 None:intergenic
AGTTTGTAGCTGCGCGTGTG+TGG 0.611019 5:-41259316 None:intergenic
CGAAGAAGCAAATCGACGTA+GGG 0.614641 5:+41259413 MsG0580026641.01.T01:CDS
ATCAAATTATAACTATCCAT+AGG 0.620605 5:-41260088 None:intergenic
GAAGCAAACAATTGTGTGTG+AGG 0.636000 5:-41259021 None:intergenic
AGAACCAAAACACATATCAA+GGG 0.643012 5:-41259577 None:intergenic
GAACCATCGCTTGGAAAGAA+CGG 0.645479 5:-41259190 None:intergenic
AAGCAAACAATTGTGTGTGA+GGG 0.669116 5:-41259020 None:intergenic
CTGCGAGAGAGTAAAACAGT+GGG 0.671795 5:-41259212 None:intergenic
ACTGCGAGAGAGTAAAACAG+TGG 0.680652 5:-41259213 None:intergenic
GCAGTTTCTGAATGTACTTG+AGG 0.702615 5:+41259788 MsG0580026641.01.T01:CDS
TGTTACTCATGACTGGAGTG+AGG 0.709741 5:+41260001 MsG0580026641.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTTATATATCCAACT+GGG + Chr5:41259660-41259679 MsG0580026641.01.T01:CDS 15.0%
!! GAAAAATTTATATATCCAAC+TGG + Chr5:41259659-41259678 MsG0580026641.01.T01:CDS 20.0%
!!! AATATTGATGATACAAAAAG+AGG - Chr5:41259844-41259863 None:intergenic 20.0%
!!! ATATGAAGTATTTTCTTCAT+TGG - Chr5:41259922-41259941 None:intergenic 20.0%
!!! TTTTGGTTCTTTTGAAAATA+AGG + Chr5:41259590-41259609 MsG0580026641.01.T01:CDS 20.0%
! AGGTTTATTTATCATTCCTA+TGG + Chr5:41260072-41260091 MsG0580026641.01.T01:CDS 25.0%
! TTGAAAACTTGATTGAACTT+CGG + Chr5:41259961-41259980 MsG0580026641.01.T01:CDS 25.0%
! TTGATGAGATTGGATAATAA+GGG - Chr5:41259720-41259739 None:intergenic 25.0%
! TTTGATGAGATTGGATAATA+AGG - Chr5:41259721-41259740 None:intergenic 25.0%
!! ACATTATAAACTGCTCTAAA+AGG - Chr5:41259768-41259787 None:intergenic 25.0%
!! TCAATCAAGTTTTCAAATAG+AGG - Chr5:41259957-41259976 None:intergenic 25.0%
!!! AAAATTAGCTTTGATGAGAT+TGG - Chr5:41259730-41259749 None:intergenic 25.0%
!!! ATTTTTCAAATCCTCCAATT+TGG - Chr5:41259646-41259665 None:intergenic 25.0%
!!! TTTTTCAAATCCTCCAATTT+GGG - Chr5:41259645-41259664 None:intergenic 25.0%
AAGAACCAAAACACATATCA+AGG - Chr5:41259581-41259600 None:intergenic 30.0%
AAGAGAGAATGTAAATGAGT+AGG - Chr5:41259140-41259159 None:intergenic 30.0%
AAGTGTTCTACAAACGAAAA+TGG - Chr5:41259484-41259503 None:intergenic 30.0%
ACTTCAAACTCTTTCGATTA+AGG + Chr5:41260052-41260071 MsG0580026641.01.T01:CDS 30.0%
AGAACCAAAACACATATCAA+GGG - Chr5:41259580-41259599 None:intergenic 30.0%
! AAAACCCTTGATATGTGTTT+TGG + Chr5:41259573-41259592 MsG0580026641.01.T01:CDS 30.0%
! ATCGAAAGAGTTTGAAGTTT+CGG - Chr5:41260050-41260069 None:intergenic 30.0%
! TTGATGATACAAAAAGAGGA+TGG - Chr5:41259840-41259859 None:intergenic 30.0%
! TTTTCTTCATTGGTAAGACT+AGG - Chr5:41259912-41259931 None:intergenic 30.0%
!! GTTCTGAAAGCATATTTGAT+CGG - Chr5:41259168-41259187 None:intergenic 30.0%
AAGCAAACAATTGTGTGTGA+GGG - Chr5:41259023-41259042 None:intergenic 35.0%
GTTCTACAAACGAAAATGGT+AGG - Chr5:41259480-41259499 None:intergenic 35.0%
TAACGAAGTCTACACGAAAA+AGG + Chr5:41259251-41259270 MsG0580026641.01.T01:CDS 35.0%
TAGCTCTCCAATGATTAGAT+TGG - Chr5:41259365-41259384 None:intergenic 35.0%
TTTCGTTTGTAGAACACTTG+TGG + Chr5:41259485-41259504 MsG0580026641.01.T01:CDS 35.0%
! TTTGAAGTTTCGGACAATTC+TGG - Chr5:41260040-41260059 None:intergenic 35.0%
! TTTTCAAAGTTCTCTATCGC+TGG - Chr5:41259696-41259715 None:intergenic 35.0%
!!! ACTCTTTTGTTGATTTCCGA+TGG - Chr5:41259072-41259091 None:intergenic 35.0%
!!! ATATCAAGGGTTTTAAGCGA+AGG - Chr5:41259567-41259586 None:intergenic 35.0%
!!! TTTTCGCTGTTTTGACACAT+GGG + Chr5:41259389-41259408 MsG0580026641.01.T01:CDS 35.0%
AACGAAAATGGTAGGCAACA+AGG - Chr5:41259472-41259491 None:intergenic 40.0%
AAGTCTACACGAAAAAGGCT+TGG + Chr5:41259256-41259275 MsG0580026641.01.T01:CDS 40.0%
AGTCTACACGAAAAAGGCTT+GGG + Chr5:41259257-41259276 MsG0580026641.01.T01:CDS 40.0%
GAAGCAAACAATTGTGTGTG+AGG - Chr5:41259024-41259043 None:intergenic 40.0%
GATTTCATGAAGCTTCTCTC+CGG + Chr5:41259615-41259634 MsG0580026641.01.T01:CDS 40.0%
GCAGTTTCTGAATGTACTTG+AGG + Chr5:41259788-41259807 MsG0580026641.01.T01:CDS 40.0%
GGTTCACTTTCGTAGAATTG+TGG + Chr5:41259278-41259297 MsG0580026641.01.T01:CDS 40.0%
TCGACTGCCAATCTAATCAT+TGG + Chr5:41259355-41259374 MsG0580026641.01.T01:CDS 40.0%
TGATTGAACTTCGGTTATCC+TGG + Chr5:41259970-41259989 MsG0580026641.01.T01:CDS 40.0%
TTTGTGTGTGATGTGCAGAT+GGG + Chr5:41259885-41259904 MsG0580026641.01.T01:intron 40.0%
!!! GTTTTCGCTGTTTTGACACA+TGG + Chr5:41259388-41259407 MsG0580026641.01.T01:CDS 40.0%
ACTGCGAGAGAGTAAAACAG+TGG - Chr5:41259216-41259235 None:intergenic 45.0%
AGCCTCTATGTCTCGTTGTT+CGG + Chr5:41259533-41259552 MsG0580026641.01.T01:CDS 45.0%
ATGAGTAACACGTGAAGTCC+AGG - Chr5:41259991-41260010 None:intergenic 45.0%
CGAAGAAGCAAATCGACGTA+GGG + Chr5:41259413-41259432 MsG0580026641.01.T01:CDS 45.0%
CTGCGAGAGAGTAAAACAGT+GGG - Chr5:41259215-41259234 None:intergenic 45.0%
GAACCATCGCTTGGAAAGAA+CGG - Chr5:41259193-41259212 None:intergenic 45.0%
GTTTGTGTGTGATGTGCAGA+TGG + Chr5:41259884-41259903 MsG0580026641.01.T01:intron 45.0%
TCAATTTCTCGTGCAGCCAT+CGG + Chr5:41259053-41259072 MsG0580026641.01.T01:CDS 45.0%
TCGAAGAAGCAAATCGACGT+AGG + Chr5:41259412-41259431 MsG0580026641.01.T01:CDS 45.0%
TGTTACTCATGACTGGAGTG+AGG + Chr5:41260001-41260020 MsG0580026641.01.T01:CDS 45.0%
TTGTGTGTGATGTGCAGATG+GGG + Chr5:41259886-41259905 MsG0580026641.01.T01:intron 45.0%
! CTTCACGTGTTACTCATGAC+TGG + Chr5:41259994-41260013 MsG0580026641.01.T01:CDS 45.0%
AAACAGTGGGAACCATCGCT+TGG - Chr5:41259202-41259221 None:intergenic 50.0%
AGAAGCAAATCGACGTAGGG+TGG + Chr5:41259416-41259435 MsG0580026641.01.T01:CDS 50.0%
CTACCGTTCTTTCCAAGCGA+TGG + Chr5:41259187-41259206 MsG0580026641.01.T01:CDS 50.0%
CTGGAAGACTGTTACCCAGT+TGG - Chr5:41259677-41259696 None:intergenic 50.0%
GACCGAACAACGAGACATAG+AGG - Chr5:41259538-41259557 None:intergenic 50.0%
TCATGACTGGAGTGAGGTAC+TGG + Chr5:41260007-41260026 MsG0580026641.01.T01:CDS 50.0%
! ATCCTCCAATTTGGGACAGC+CGG - Chr5:41259637-41259656 None:intergenic 50.0%
AGCAAATCGACGTAGGGTGG+AGG + Chr5:41259419-41259438 MsG0580026641.01.T01:CDS 55.0%
AGTTTGTAGCTGCGCGTGTG+TGG - Chr5:41259319-41259338 None:intergenic 55.0%
TCTCTCCGGCTGTCCCAAAT+TGG + Chr5:41259629-41259648 MsG0580026641.01.T01:CDS 55.0%
CTCCGGCTGTCCCAAATTGG+AGG + Chr5:41259632-41259651 MsG0580026641.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 41259006 41260109 41259006 ID=MsG0580026641.01;Name=MsG0580026641.01
Chr5 mRNA 41259006 41260109 41259006 ID=MsG0580026641.01.T01;Parent=MsG0580026641.01;Name=MsG0580026641.01.T01;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|1|2|0|336
Chr5 exon 41259006 41259809 41259006 ID=MsG0580026641.01.T01:exon:9870;Parent=MsG0580026641.01.T01
Chr5 exon 41259903 41260109 41259903 ID=MsG0580026641.01.T01:exon:9871;Parent=MsG0580026641.01.T01
Chr5 CDS 41259006 41259809 41259006 ID=MsG0580026641.01.T01:cds;Parent=MsG0580026641.01.T01
Chr5 CDS 41259903 41260109 41259903 ID=MsG0580026641.01.T01:cds;Parent=MsG0580026641.01.T01
Gene Sequence

>MsG0580026641.01.T01

ATGCACGTTCACAACCCTCACACACAATTGTTTGCTTCATTAGCGTATCAATTTCTCGTGCAGCCATCGGAAATCAACAAAAGAGTAAAACATACGACAAAGTCGTGTTCAATCAACGATCTTATAGACGACCTACTCATTTACATTCTCTCTTTTCTTCCGATCAAATATGCTTTCAGAACTACCGTTCTTTCCAAGCGATGGTTCCCACTGTTTTACTCTCTCGCAGTTATTCGCATATTCGATAACGAAGTCTACACGAAAAAGGCTTGGGTTCACTTTCGTAGAATTGTGGACAAAGTTATACTCTCCACACACGCGCAGCTACAAACTCTTAAAACATTTCATCTCGACTGCCAATCTAATCATTGGAGAGCTAATAGTTTTCGCTGTTTTGACACATGGGTCGAAGAAGCAAATCGACGTAGGGTGGAGGATCTCTATCTAAACTTGTTCGAAGTAACCTTGTTGCCTACCATTTTCGTTTGTAGAACACTTGTGGTTCTCAAATTTGATAGCTTACGTGTAGCCTCTATGTCTCGTTGTTCGGTCGATCTTCCTTCGCTTAAAACCCTTGATATGTGTTTTGGTTCTTTTGAAAATAAGGATGATTTCATGAAGCTTCTCTCCGGCTGTCCCAAATTGGAGGATTTGAAAAATTTATATATCCAACTGGGTAACAGTCTTCCAGCGATAGAGAACTTTGAAAAACCCTTATTATCCAATCTCATCAAAGCTAATTTTCGTTTATTCGAAGTTCCTTTTAGAGCAGTTTATAATGTGCAGTTTCTGAATGTACTTGAGATGGGGCCTAGTCTTACCAATGAAGAAAATACTTCATATTACAAAATCTTACCTCTATTTGAAAACTTGATTGAACTTCGGTTATCCTGGACTTCACGTGTTACTCATGACTGGAGTGAGGTACTGGAAATGCTCCAGAATTGTCCGAAACTTCAAACTCTTTCGATTAAGGTTTATTTATCATTCCTATGGATAGTTATAATTTGA
Protein sequence

>MsG0580026641.01.T01

MHVHNPHTQLFASLAYQFLVQPSEINKRVKHTTKSCSINDLIDDLLIYILSFLPIKYAFRTTVLSKRWFPLFYSLAVIRIFDNEVYTKKAWVHFRRIVDKVILSTHAQLQTLKTFHLDCQSNHWRANSFRCFDTWVEEANRRRVEDLYLNLFEVTLLPTIFVCRTLVVLKFDSLRVASMSRCSVDLPSLKTLDMCFGSFENKDDFMKLLSGCPKLEDLKNLYIQLGNSLPAIENFEKPLLSNLIKANFRLFEVPFRAVYNVQFLNVLEMGPSLTNEENTSYYKILPLFENLIELRLSWTSRVTHDWSEVLEMLQNCPKLQTLSIKVYLSFLWIVII*