AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028939.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028939.01.T01 MTR_5g058000 67.277 382 87 3 1 351 1 375 2.11e-165 467
MsG0580028939.01.T01 MTR_5g058100 63.281 384 108 4 1 351 1 384 1.25e-154 440
MsG0580028939.01.T01 MTR_1g066830 53.886 386 142 4 1 350 1 386 7.23e-122 357
MsG0580028939.01.T01 MTR_1089s0010 51.421 387 148 6 1 353 1 381 9.65e-115 338
MsG0580028939.01.T01 MTR_5g073420 47.680 388 153 7 1 350 4 379 1.80e-104 312
MsG0580028939.01.T01 MTR_6g040270 48.042 383 161 4 1 349 1 379 2.87e-102 306
MsG0580028939.01.T01 MTR_1g085120 47.507 381 160 8 8 350 92 470 3.35e-93 286
MsG0580028939.01.T01 MTR_1g085120 47.507 381 160 8 8 350 167 545 1.83e-92 286
MsG0580028939.01.T01 MTR_4g039440 45.288 382 168 9 7 350 33 411 6.12e-87 268
MsG0580028939.01.T01 MTR_6g022500 45.093 377 171 6 7 349 100 474 2.13e-86 269
MsG0580028939.01.T01 MTR_6g011460 45.220 387 168 9 2 350 40 420 1.10e-82 258
MsG0580028939.01.T01 MTR_1g108820 42.239 393 178 10 1 351 1 386 2.65e-81 253
MsG0580028939.01.T01 MTR_7g055943 41.645 389 181 8 4 350 36 420 5.71e-77 243
MsG0580028939.01.T01 MTR_5g045170 40.568 387 184 8 4 350 10 390 5.31e-75 237
MsG0580028939.01.T01 MTR_6g045333 39.798 397 185 10 2 350 55 445 5.08e-74 236
MsG0580028939.01.T01 MTR_7g026500 39.055 402 183 8 1 352 7 396 3.97e-73 232
MsG0580028939.01.T01 MTR_1g108800 44.904 363 167 10 1 351 1 342 4.05e-72 228
MsG0580028939.01.T01 MTR_6g022360 40.691 376 183 6 7 351 42 408 1.25e-70 226
MsG0580028939.01.T01 MTR_5g061680 41.429 350 144 6 2 318 5 326 2.43e-69 220
MsG0580028939.01.T01 MTR_5g007410 38.667 375 187 6 10 343 28 400 1.23e-68 222
MsG0580028939.01.T01 MTR_0009s0080 39.842 379 183 9 1 337 11 386 1.69e-68 221
MsG0580028939.01.T01 MTR_6g044572 40.389 411 154 8 7 350 1 387 3.92e-68 219
MsG0580028939.01.T01 MTR_6g044560 40.144 416 158 8 2 350 172 563 6.26e-67 221
MsG0580028939.01.T01 MTR_6g022520 49.645 282 135 5 75 350 32 312 3.20e-65 209
MsG0580028939.01.T01 MTR_2g036470 38.021 384 195 5 8 350 14 395 1.84e-64 210
MsG0580028939.01.T01 MTR_3g071850 36.883 385 197 8 7 347 23 405 3.67e-63 216
MsG0580028939.01.T01 MTR_5g073340 42.904 303 154 8 1 287 1 300 1.94e-62 202
MsG0580028939.01.T01 MTR_5g073370 38.287 397 186 13 1 353 1 382 2.56e-62 204
MsG0580028939.01.T01 MTR_1g085120 46.575 292 117 7 8 262 179 468 1.10e-61 204
MsG0580028939.01.T01 MTR_3g095600 41.143 350 128 10 7 340 3 290 1.59e-61 199
MsG0580028939.01.T01 MTR_5g076150 50.455 220 85 5 85 283 11 227 1.70e-60 194
MsG0580028939.01.T01 MTR_5g031970 37.831 378 170 10 3 349 11 354 4.55e-58 192
MsG0580028939.01.T01 MTR_5g045510 37.435 382 182 11 7 351 11 372 1.21e-57 191
MsG0580028939.01.T01 MTR_1g108820 40.132 304 138 7 1 264 1 300 4.13e-54 181
MsG0580028939.01.T01 MTR_5g039490 34.783 391 184 8 4 352 16 377 4.61e-54 182
MsG0580028939.01.T01 MTR_5g039450 35.696 381 182 11 4 348 2 355 4.61e-53 179
MsG0580028939.01.T01 MTR_5g039470 37.500 328 169 8 24 315 1 328 9.63e-51 172
MsG0580028939.01.T01 MTR_3g095630 52.973 185 77 7 170 350 7 185 1.10e-49 165
MsG0580028939.01.T01 MTR_5g063950 32.603 365 209 7 8 348 8 359 1.63e-49 170
MsG0580028939.01.T01 MTR_7g056433 34.518 394 208 9 1 350 1 388 1.04e-47 166
MsG0580028939.01.T01 MTR_5g058350 54.494 178 73 3 1 170 1 178 5.95e-47 157
MsG0580028939.01.T01 MTR_7g098510 32.208 385 221 9 1 350 11 390 5.61e-45 159
MsG0580028939.01.T01 MTR_7g088260 33.679 386 212 9 1 350 84 461 2.26e-44 159
MsG0580028939.01.T01 MTR_5g033200 53.939 165 69 4 179 340 11 171 5.60e-44 150
MsG0580028939.01.T01 MTR_1377s0010 62.205 127 43 3 193 318 5 127 5.76e-44 148
MsG0580028939.01.T01 MTR_5g065100 33.590 390 153 11 13 350 13 348 1.58e-43 156
MsG0580028939.01.T01 MTR_3g107120 32.494 397 195 15 8 348 9 388 4.41e-42 151
MsG0580028939.01.T01 MTR_1g041245 32.990 388 204 13 7 350 10 385 2.67e-41 149
MsG0580028939.01.T01 MTR_3g107100 33.165 395 204 12 8 350 11 397 4.07e-41 149
MsG0580028939.01.T01 MTR_7g098570 30.809 383 219 9 2 346 14 388 5.54e-41 150
MsG0580028939.01.T01 MTR_3g108560 33.773 379 205 10 8 350 20 388 1.61e-40 147
MsG0580028939.01.T01 MTR_7g033990 34.211 380 187 13 7 340 10 372 9.60e-39 142
MsG0580028939.01.T01 MTR_7g098480 32.361 377 205 11 1 338 11 376 1.37e-38 143
MsG0580028939.01.T01 MTR_1g053025 31.658 398 204 14 7 348 1 386 1.71e-38 141
MsG0580028939.01.T01 MTR_2g007450 31.265 419 205 13 3 349 11 418 2.32e-38 142
MsG0580028939.01.T01 MTR_3g105060 31.117 376 211 9 7 350 13 372 3.47e-38 140
MsG0580028939.01.T01 MTR_7g033915 30.649 385 196 11 7 350 10 364 4.63e-38 140
MsG0580028939.01.T01 MTR_0543s0010 31.915 376 211 8 8 350 86 449 6.33e-38 141
MsG0580028939.01.T01 MTR_3g099830 33.333 387 200 18 2 349 5 372 3.63e-37 137
MsG0580028939.01.T01 MTR_2g007400 30.825 412 205 14 3 350 7 402 3.70e-37 138
MsG0580028939.01.T01 MTR_7g032380 31.458 391 187 14 2 350 4 355 8.32e-37 136
MsG0580028939.01.T01 MTR_7g007080 33.079 393 193 15 8 349 4 377 1.06e-36 137
MsG0580028939.01.T01 MTR_3g108150 32.723 382 210 8 8 350 13 386 1.38e-36 136
MsG0580028939.01.T01 MTR_0492s0020 31.768 362 202 8 8 336 86 435 1.86e-36 137
MsG0580028939.01.T01 MTR_3g036250 32.637 383 192 16 8 348 5 363 2.47e-36 135
MsG0580028939.01.T01 MTR_3g023630 31.768 362 186 11 1 318 1 345 7.04e-36 134
MsG0580028939.01.T01 MTR_7g115270 29.847 392 220 11 8 348 12 399 1.74e-35 134
MsG0580028939.01.T01 MTR_5g054920 40.299 201 103 5 143 335 2 193 2.29e-35 128
MsG0580028939.01.T01 MTR_2g005990 31.477 413 195 16 4 349 2 393 5.62e-35 132
MsG0580028939.01.T01 MTR_7g007340 33.983 359 182 15 2 329 6 340 7.06e-35 131
MsG0580028939.01.T01 MTR_1g107385 31.373 408 196 17 8 349 32 421 4.46e-34 130
MsG0580028939.01.T01 MTR_7g056277 31.266 387 204 11 8 350 259 627 5.44e-34 132
MsG0580028939.01.T01 MTR_7g056287 31.552 393 195 12 8 350 3 371 6.51e-34 129
MsG0580028939.01.T01 MTR_3g040700 31.686 344 176 10 8 311 43 367 7.86e-34 130
MsG0580028939.01.T01 MTR_3g107160 29.974 387 209 13 8 348 10 380 1.44e-33 128
MsG0580028939.01.T01 MTR_7g075820 32.020 406 193 16 8 349 6 392 1.84e-33 128
MsG0580028939.01.T01 MTR_2g008310 32.115 383 206 13 7 349 1 369 2.46e-33 127
MsG0580028939.01.T01 MTR_5g008950 31.383 376 211 10 10 350 1 364 1.43e-32 125
MsG0580028939.01.T01 MTR_2g007360 32.283 381 209 16 3 349 7 372 2.50e-32 124
MsG0580028939.01.T01 MTR_1g059860 33.438 317 154 13 40 311 7 311 3.31e-32 123
MsG0580028939.01.T01 MTR_4g098590 30.871 379 192 14 10 330 1 367 9.70e-32 123
MsG0580028939.01.T01 MTR_5g008920 32.564 390 209 14 1 350 5 380 2.90e-31 122
MsG0580028939.01.T01 MTR_2g008320 30.964 394 208 14 4 349 48 425 3.19e-31 122
MsG0580028939.01.T01 MTR_5g083890 31.343 402 191 16 8 340 6 391 4.77e-31 123
MsG0580028939.01.T01 MTR_7g034115 31.283 374 199 14 3 332 5 364 5.61e-31 121
MsG0580028939.01.T01 MTR_7g083740 29.521 376 220 11 2 350 9 366 6.22e-31 120
MsG0580028939.01.T01 MTR_2g007500 31.515 330 185 9 2 295 8 332 1.04e-30 119
MsG0580028939.01.T01 MTR_3g082890 29.457 387 206 13 8 337 97 473 7.03e-30 119
MsG0580028939.01.T01 MTR_3g095640 42.424 165 56 5 1 126 11 175 7.97e-30 112
MsG0580028939.01.T01 MTR_7g032370 30.601 366 203 11 20 350 2 351 1.49e-28 114
MsG0580028939.01.T01 MTR_2g007090 30.532 357 167 13 5 350 7 293 1.01e-27 110
MsG0580028939.01.T01 MTR_3g071790 34.946 186 110 4 136 312 19 202 1.35e-27 107
MsG0580028939.01.T01 MTR_5g012840 30.704 355 158 14 33 350 14 317 1.41e-27 110
MsG0580028939.01.T01 MTR_7g007370 33.146 356 185 13 7 347 16 333 1.85e-27 110
MsG0580028939.01.T01 MTR_7g056420 27.733 375 196 11 8 338 9 352 8.13e-27 112
MsG0580028939.01.T01 MTR_7g056430 27.733 375 196 11 8 338 9 352 9.40e-27 112
MsG0580028939.01.T01 MTR_3g087260 32.776 299 169 8 70 350 172 456 1.10e-26 110
MsG0580028939.01.T01 MTR_3g087260 33.456 272 145 10 8 261 12 265 8.10e-20 90.5
MsG0580028939.01.T01 MTR_3g082890 28.774 424 198 14 8 337 17 430 1.20e-26 110
MsG0580028939.01.T01 MTR_7g060470 30.412 388 203 14 8 338 3 380 1.24e-26 109
MsG0580028939.01.T01 MTR_7g034120 34.323 303 160 14 68 350 24 307 2.99e-26 107
MsG0580028939.01.T01 MTR_3g108560 34.375 288 146 9 8 262 20 297 1.17e-25 105
MsG0580028939.01.T01 MTR_5g061410 33.457 269 159 7 8 262 5 267 1.65e-25 103
MsG0580028939.01.T01 MTR_7g021990 28.302 371 216 9 8 336 48 410 1.90e-24 103
MsG0580028939.01.T01 MTR_7g114710 29.610 385 197 15 7 350 12 363 2.89e-24 102
MsG0580028939.01.T01 MTR_7g056020 30.518 367 196 11 8 337 16 360 3.49e-24 103
MsG0580028939.01.T01 MTR_7g021950 28.302 371 216 9 8 336 48 410 3.61e-24 103
MsG0580028939.01.T01 MTR_7g022105 28.533 368 219 9 8 336 49 411 8.95e-24 101
MsG0580028939.01.T01 MTR_4g080610 29.301 372 216 12 2 350 9 356 4.05e-22 96.3
MsG0580028939.01.T01 MTR_7g056447 36.413 184 87 4 1 154 1 184 4.56e-22 95.1
MsG0580028939.01.T01 MTR_5g024060 42.400 125 66 3 222 343 79 200 1.34e-21 92.8
MsG0580028939.01.T01 MTR_3g026920 27.078 421 196 13 5 348 16 402 4.34e-21 94.0
MsG0580028939.01.T01 MTR_3g109982 33.205 259 152 9 92 339 215 463 1.37e-20 93.6
MsG0580028939.01.T01 MTR_3g109982 30.877 285 152 8 8 261 13 283 4.06e-20 92.0
MsG0580028939.01.T01 MTR_3g110010 30.877 285 152 8 8 261 13 283 6.49e-20 91.7
MsG0580028939.01.T01 MTR_7g034110 25.575 348 176 10 7 318 10 310 3.87e-19 87.8
MsG0580028939.01.T01 MTR_6g022410 46.512 86 46 0 265 350 21 106 4.40e-18 79.3
MsG0580028939.01.T01 MTR_6g029350 34.404 218 107 11 147 350 2 197 8.23e-18 82.0
MsG0580028939.01.T01 MTR_6g033095 56.000 75 32 1 245 318 110 184 1.29e-17 80.5
MsG0580028939.01.T01 MTR_5g044790 35.294 153 61 3 231 349 290 438 2.37e-17 83.2
MsG0580028939.01.T01 MTR_2g007550 27.419 372 186 13 8 348 14 332 1.09e-16 80.5
MsG0580028939.01.T01 MTR_6g022390 40.000 130 40 2 7 98 4 133 2.48e-16 75.9
MsG0580028939.01.T01 MTR_5g013500 28.620 297 183 11 69 350 82 364 2.80e-16 79.3
MsG0580028939.01.T01 MTR_4g093690 34.066 182 84 7 8 154 18 198 1.18e-15 76.6
MsG0580028939.01.T01 MTR_0571s0010 65.574 61 19 2 4 64 29 87 1.40e-15 72.4
MsG0580028939.01.T01 MTR_5g080710 26.279 430 225 16 7 351 32 454 3.80e-15 76.6
MsG0580028939.01.T01 MTR_8g062310 24.700 417 236 14 3 350 20 427 7.25e-15 75.9
MsG0580028939.01.T01 MTR_7g034090 29.016 386 159 15 8 353 4 314 1.43e-14 74.7
MsG0580028939.01.T01 MTR_5g073400 40.984 122 63 4 67 181 19 138 3.22e-14 69.3
MsG0580028939.01.T01 MTR_3g115430 33.333 111 69 2 242 350 17 124 1.63e-13 67.0
MsG0580028939.01.T01 MTR_4g134150 32.065 184 94 5 8 164 14 193 1.71e-13 69.7
MsG0580028939.01.T01 MTR_2g020180 34.711 121 65 3 223 337 92 204 6.87e-13 67.4
MsG0580028939.01.T01 MTR_5g039950 24.064 374 212 12 8 318 23 387 7.59e-13 69.7
MsG0580028939.01.T01 MTR_6g083130 44.086 93 40 2 73 164 233 314 9.00e-13 68.6
MsG0580028939.01.T01 MTR_2g007610 32.857 140 75 6 218 349 21 149 9.33e-13 66.2
MsG0580028939.01.T01 MTR_8g467800 24.773 440 227 15 8 350 20 452 1.33e-12 68.9
MsG0580028939.01.T01 MTR_7g022060 32.512 203 111 6 8 184 10 212 1.60e-12 68.2
MsG0580028939.01.T01 MTR_7g021930 32.512 203 111 6 8 184 10 212 1.79e-12 67.8
MsG0580028939.01.T01 MTR_7g021970 32.512 203 111 6 8 184 10 212 1.79e-12 67.8
MsG0580028939.01.T01 MTR_5g092960 28.000 275 169 8 84 350 208 461 2.57e-12 68.2
MsG0580028939.01.T01 MTR_7g033945 32.121 165 75 4 7 135 10 173 4.53e-12 64.3
MsG0580028939.01.T01 MTR_5g045700 56.522 46 20 0 10 55 15 60 5.21e-12 60.8
MsG0580028939.01.T01 MTR_3g106960 32.099 162 91 9 174 323 23 177 6.91e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028939.01.T01 AT5G56820 27.737 411 225 13 8 348 15 423 8.40e-20 90.5
MsG0580028939.01.T01 AT4G00160 26.895 409 212 15 8 334 16 419 2.72e-19 89.0
MsG0580028939.01.T01 AT5G56420 25.714 420 231 12 8 350 6 421 5.94e-19 87.8
MsG0580028939.01.T01 AT5G56420 25.714 420 231 12 8 350 6 421 5.94e-19 87.8
MsG0580028939.01.T01 AT5G56420 25.714 420 231 12 8 350 6 421 5.94e-19 87.8
MsG0580028939.01.T01 AT3G50710 25.701 428 228 13 7 348 1 424 4.01e-18 85.5
MsG0580028939.01.T01 AT5G56370 26.230 427 227 16 7 351 1 421 2.28e-16 80.1
MsG0580028939.01.T01 AT5G56370 26.230 427 227 16 7 351 1 421 2.28e-16 80.1
MsG0580028939.01.T01 AT3G56780 33.146 178 86 4 10 157 9 183 6.95e-15 75.9
MsG0580028939.01.T01 AT4G00320 35.859 198 77 9 8 157 13 208 8.15e-15 75.9
MsG0580028939.01.T01 AT3G56780 33.146 178 86 4 10 157 9 183 8.58e-15 75.5
MsG0580028939.01.T01 AT3G56780 33.146 178 86 4 10 157 9 183 1.39e-14 74.7
MsG0580028939.01.T01 AT5G56400 25.117 426 234 14 7 350 32 454 1.87e-14 74.7
MsG0580028939.01.T01 AT5G60610 32.402 179 91 5 7 157 1 177 8.47e-14 72.4
MsG0580028939.01.T01 AT5G60610 32.402 179 91 5 7 157 1 177 8.47e-14 72.4
MsG0580028939.01.T01 AT4G15060 30.052 193 99 5 7 164 25 216 9.75e-14 72.4
MsG0580028939.01.T01 AT4G15060 30.052 193 99 5 7 164 171 362 1.33e-13 72.4
MsG0580028939.01.T01 AT3G58860 26.462 359 166 12 3 270 2 353 1.42e-13 72.0
MsG0580028939.01.T01 AT3G49150 32.447 188 87 7 8 157 16 201 5.73e-13 70.1
MsG0580028939.01.T01 AT5G22670 31.925 213 108 6 5 187 8 213 9.18e-13 69.3
MsG0580028939.01.T01 AT5G53635 30.601 183 91 5 9 158 1 180 4.31e-12 67.4
MsG0580028939.01.T01 AT5G56380 31.746 189 90 4 7 157 1 188 9.86e-12 66.2
MsG0580028939.01.T01 AT5G56380 31.746 189 90 4 7 157 1 188 9.86e-12 66.2
MsG0580028939.01.T01 AT5G25850 31.111 180 93 5 7 158 19 195 1.30e-11 65.9
MsG0580028939.01.T01 AT5G53640 27.273 176 101 4 8 157 22 196 1.38e-11 63.9
MsG0580028939.01.T01 AT5G56370 31.638 177 92 3 7 154 1 177 1.42e-11 65.1
MsG0580028939.01.T01 AT1G55660 32.979 188 87 8 7 157 52 237 2.64e-11 64.7
MsG0580028939.01.T01 AT1G51370 30.055 183 103 4 8 166 19 200 2.66e-11 64.7
MsG0580028939.01.T01 AT1G51370 29.570 186 106 4 8 169 19 203 3.00e-11 64.7
MsG0580028939.01.T01 AT1G55660 32.979 188 87 8 7 157 52 237 3.08e-11 64.7
MsG0580028939.01.T01 AT1G51370 29.570 186 106 4 8 169 19 203 3.72e-11 64.3
MsG0580028939.01.T01 AT3G59250 34.615 156 97 4 6 157 5 159 5.46e-11 63.9
MsG0580028939.01.T01 AT5G22660 33.708 178 89 5 8 157 13 189 7.59e-11 62.8

Find 58 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATTTAATAACCGAATT+TGG 0.060735 5:+89059010 None:intergenic
ATCCTTGTTCTTGTCCAAAT+TGG 0.227398 5:-89059381 MsG0580028939.01.T01:CDS
ATTTGGGTATCTCCATTTAT+TGG 0.242394 5:+89059027 None:intergenic
TTTGAAGCTTGGGACAATTC+TGG 0.266893 5:+89059165 None:intergenic
TTTGAGAACAACTAGGGTTT+TGG 0.270755 5:+89059604 None:intergenic
CTAGTTGTTCTCAAACTTGA+TGG 0.272062 5:-89059596 MsG0580028939.01.T01:CDS
GCAGAGTGGGGCCCATCTTT+TGG 0.276573 5:+89059891 None:intergenic
GTTCCTGCTTATCCAATAAA+TGG 0.278720 5:-89059039 MsG0580028939.01.T01:CDS
AAAATGGGCTCTAACCAATT+TGG 0.286895 5:+89059367 None:intergenic
ACTTAATTCATATTCAACTA+TGG 0.298184 5:-89059232 MsG0580028939.01.T01:CDS
CACATTTAATAACCGAATTT+GGG 0.303259 5:+89059011 None:intergenic
GATCTAAGCATAGTGAATTA+TGG 0.339416 5:-89059302 MsG0580028939.01.T01:CDS
TGTTTGACTTCTTTCAAGGC+TGG 0.349907 5:-89059208 MsG0580028939.01.T01:CDS
GCAAATGAAAGTTTGCATTC+AGG 0.351773 5:+89058800 None:intergenic
GCAAACTTTCATTTGCATCT+AGG 0.361613 5:-89058793 MsG0580028939.01.T01:CDS
ACATTAAACGGAATATCATT+TGG 0.371762 5:+89059341 None:intergenic
ACATTAGAAATTACATTAAA+CGG 0.375538 5:+89059329 None:intergenic
GGTGGTGCGGAAAGCAAGTT+TGG 0.375662 5:+89059921 None:intergenic
TGGTTGTTTGACTTCTTTCA+AGG 0.384531 5:-89059212 MsG0580028939.01.T01:CDS
TCATTATGTGCATCAGGTCT+TGG 0.399980 5:+89059419 None:intergenic
TCAAAATCTTCGTATTAGAA+AGG 0.429008 5:-89059147 MsG0580028939.01.T01:intron
TCCGCTTCATTATGTGCATC+AGG 0.429959 5:+89059413 None:intergenic
GTTTGACTTCTTTCAAGGCT+GGG 0.430465 5:-89059207 MsG0580028939.01.T01:CDS
TAAGTTGCTGATCATATCAA+TGG 0.454253 5:+89059984 None:intergenic
GACTTCTTTCAAGGCTGGGA+TGG 0.458053 5:-89059203 MsG0580028939.01.T01:CDS
CCACCCTTCTCTCCAAAAGA+TGG 0.459226 5:-89059903 MsG0580028939.01.T01:CDS
TCTCCATTTATTGGATAAGC+AGG 0.469343 5:+89059036 None:intergenic
TAAATGGAGATACCCAAATT+CGG 0.483611 5:-89059023 MsG0580028939.01.T01:CDS
CATCAAGTTTGAGAACAACT+AGG 0.485505 5:+89059597 None:intergenic
TTGAAAGGATTGCCTTTCAA+AGG 0.499323 5:+89059275 None:intergenic
TTGAAAGGCAATCCTTTCAA+AGG 0.502827 5:-89059272 MsG0580028939.01.T01:CDS
TAATGTAATTTCTAATGTTG+AGG 0.503146 5:-89059324 MsG0580028939.01.T01:CDS
GAAGCCGTCAGCATCTTTCA+CGG 0.504546 5:+89059834 None:intergenic
AACTAACTTGACGCAAATGT+AGG 0.515954 5:+89059522 None:intergenic
AAGATTTATCCTCCTTTCCA+TGG 0.516532 5:-89058829 MsG0580028939.01.T01:CDS
TTGCATTCAGGCGACATCCA+TGG 0.517644 5:+89058812 None:intergenic
AACACAGGAAAGCCTTTGAA+AGG 0.520947 5:+89059260 None:intergenic
GTGCGGAAAGCAAGTTTGGT+TGG 0.529155 5:+89059925 None:intergenic
ATCAAGTTTGAGAACAACTA+GGG 0.534200 5:+89059598 None:intergenic
ATAGTCAAAGCTTGGATGCA+TGG 0.543845 5:-89059709 MsG0580028939.01.T01:CDS
AATTATGGAGAGCCTTTGAA+AGG 0.544393 5:-89059287 MsG0580028939.01.T01:CDS
CACCCTTCTCTCCAAAAGAT+GGG 0.544584 5:-89059902 MsG0580028939.01.T01:CDS
TGAAGTTGATAGTCAAAGCT+TGG 0.545526 5:-89059717 MsG0580028939.01.T01:intron
TGCACATAATGAAGCGGAAG+AGG 0.548812 5:-89059408 MsG0580028939.01.T01:CDS
ACTAACTTGACGCAAATGTA+GGG 0.552868 5:+89059523 None:intergenic
AAGTTGCTGATCATATCAAT+GGG 0.558937 5:+89059985 None:intergenic
TAGTCAAAGCTTGGATGCAT+GGG 0.559042 5:-89059708 MsG0580028939.01.T01:CDS
TGAAAATAGTACATGATCTG+AGG 0.582616 5:+89058978 None:intergenic
AACCAATTTGGACAAGAACA+AGG 0.589070 5:+89059379 None:intergenic
ACCTGATGCACATAATGAAG+CGG 0.596618 5:-89059414 MsG0580028939.01.T01:CDS
TTCAGGCGACATCCATGGAA+AGG 0.599676 5:+89058817 None:intergenic
TTGTGAATGAGTTGCAGAGT+GGG 0.615940 5:+89059878 None:intergenic
AGGCGACATCCATGGAAAGG+AGG 0.649547 5:+89058820 None:intergenic
AGCCGCAAAACAACGCCACG+TGG 0.659312 5:-89059681 MsG0580028939.01.T01:CDS
TGTGAATGAGTTGCAGAGTG+GGG 0.684778 5:+89059879 None:intergenic
GTTGTGAATGAGTTGCAGAG+TGG 0.716400 5:+89059877 None:intergenic
GATACCGTGAAAGATGCTGA+CGG 0.730540 5:-89059838 MsG0580028939.01.T01:CDS
AGAGACGAAAATTCTCCACG+TGG 0.788206 5:+89059666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTAGAAATTACATTAAA+CGG + Chr5:89059464-89059483 None:intergenic 15.0%
!! AATATCATTTGGACAAAAAT+GGG + Chr5:89059441-89059460 None:intergenic 20.0%
!! ACTTAATTCATATTCAACTA+TGG - Chr5:89059558-89059577 MsG0580028939.01.T01:CDS 20.0%
!! TAATGTAATTTCTAATGTTG+AGG - Chr5:89059466-89059485 MsG0580028939.01.T01:CDS 20.0%
!!! GTACTATTTTCAATTTTGAT+GGG - Chr5:89059823-89059842 MsG0580028939.01.T01:intron 20.0%
!!! TGTACTATTTTCAATTTTGA+TGG - Chr5:89059822-89059841 MsG0580028939.01.T01:intron 20.0%
! ACACATTTAATAACCGAATT+TGG + Chr5:89059783-89059802 None:intergenic 25.0%
! ACATTAAACGGAATATCATT+TGG + Chr5:89059452-89059471 None:intergenic 25.0%
! CACATTTAATAACCGAATTT+GGG + Chr5:89059782-89059801 None:intergenic 25.0%
! GAATATCATTTGGACAAAAA+TGG + Chr5:89059442-89059461 None:intergenic 25.0%
! TCAAAATCTTCGTATTAGAA+AGG - Chr5:89059643-89059662 MsG0580028939.01.T01:CDS 25.0%
!! GTTTTCAAATCTAGTAGAAT+TGG + Chr5:89059335-89059354 None:intergenic 25.0%
!!! TGAATTAAGTTTTGAAACAC+AGG + Chr5:89059548-89059567 None:intergenic 25.0%
AAGTTGCTGATCATATCAAT+GGG + Chr5:89058808-89058827 None:intergenic 30.0%
ATCAAGTTTGAGAACAACTA+GGG + Chr5:89059195-89059214 None:intergenic 30.0%
ATTTGGGTATCTCCATTTAT+TGG + Chr5:89059766-89059785 None:intergenic 30.0%
GATCTAAGCATAGTGAATTA+TGG - Chr5:89059488-89059507 MsG0580028939.01.T01:CDS 30.0%
TAAATGGAGATACCCAAATT+CGG - Chr5:89059767-89059786 MsG0580028939.01.T01:intron 30.0%
TAAGTTGCTGATCATATCAA+TGG + Chr5:89058809-89058828 None:intergenic 30.0%
TGAAAATAGTACATGATCTG+AGG + Chr5:89059815-89059834 None:intergenic 30.0%
! TGCTTTTGCATTTCATTTGA+AGG + Chr5:89059930-89059949 None:intergenic 30.0%
!! TTAGGTGAAACGTTTTAATG+AGG + Chr5:89059028-89059047 None:intergenic 30.0%
!!! AATACGAAGATTTTGAAGCT+TGG + Chr5:89059639-89059658 None:intergenic 30.0%
!!! ATACGAAGATTTTGAAGCTT+GGG + Chr5:89059638-89059657 None:intergenic 30.0%
!!! GTCAAGTTAGTTTTCACAAT+CGG - Chr5:89059279-89059298 MsG0580028939.01.T01:CDS 30.0%
!!! GTTTTGGAGATGAAAATTGT+GGG + Chr5:89059173-89059192 None:intergenic 30.0%
AACCAATTTGGACAAGAACA+AGG + Chr5:89059414-89059433 None:intergenic 35.0%
AACTAACTTGACGCAAATGT+AGG + Chr5:89059271-89059290 None:intergenic 35.0%
AAGATTTATCCTCCTTTCCA+TGG - Chr5:89059961-89059980 MsG0580028939.01.T01:CDS 35.0%
AATTATGGAGAGCCTTTGAA+AGG - Chr5:89059503-89059522 MsG0580028939.01.T01:CDS 35.0%
ACTAACTTGACGCAAATGTA+GGG + Chr5:89059270-89059289 None:intergenic 35.0%
ATCCTTGTTCTTGTCCAAAT+TGG - Chr5:89059409-89059428 MsG0580028939.01.T01:CDS 35.0%
CATCAAGTTTGAGAACAACT+AGG + Chr5:89059196-89059215 None:intergenic 35.0%
CTAGTTGTTCTCAAACTTGA+TGG - Chr5:89059194-89059213 MsG0580028939.01.T01:CDS 35.0%
GCAAACTTTCATTTGCATCT+AGG - Chr5:89059997-89060016 MsG0580028939.01.T01:CDS 35.0%
GCAAATGAAAGTTTGCATTC+AGG + Chr5:89059993-89060012 None:intergenic 35.0%
GTTCCTGCTTATCCAATAAA+TGG - Chr5:89059751-89059770 MsG0580028939.01.T01:intron 35.0%
TCTCCATTTATTGGATAAGC+AGG + Chr5:89059757-89059776 None:intergenic 35.0%
TTGAAAGGATTGCCTTTCAA+AGG + Chr5:89059518-89059537 None:intergenic 35.0%
TTGAAAGGCAATCCTTTCAA+AGG - Chr5:89059518-89059537 MsG0580028939.01.T01:CDS 35.0%
! AAAATGGGCTCTAACCAATT+TGG + Chr5:89059426-89059445 None:intergenic 35.0%
! GAGAGAATCGATCGAATTTT+AGG + Chr5:89059046-89059065 None:intergenic 35.0%
! TGGTTGTTTGACTTCTTTCA+AGG - Chr5:89059578-89059597 MsG0580028939.01.T01:CDS 35.0%
! TTTGAGAACAACTAGGGTTT+TGG + Chr5:89059189-89059208 None:intergenic 35.0%
!! AAACGTTTTAATGAGGCATG+AGG + Chr5:89059021-89059040 None:intergenic 35.0%
!! CGTATTAGAAAGGTTTGTTG+AGG - Chr5:89059653-89059672 MsG0580028939.01.T01:CDS 35.0%
!! TGAAGTTGATAGTCAAAGCT+TGG - Chr5:89059073-89059092 MsG0580028939.01.T01:intron 35.0%
!!! AAATGTAGGGTTTTGAGAGA+TGG + Chr5:89059257-89059276 None:intergenic 35.0%
!!! GGTTTTGGAGATGAAAATTG+TGG + Chr5:89059174-89059193 None:intergenic 35.0%
AACACAGGAAAGCCTTTGAA+AGG + Chr5:89059533-89059552 None:intergenic 40.0%
ACCTGATGCACATAATGAAG+CGG - Chr5:89059376-89059395 MsG0580028939.01.T01:CDS 40.0%
ATAGTCAAAGCTTGGATGCA+TGG - Chr5:89059081-89059100 MsG0580028939.01.T01:intron 40.0%
CCACTGTCAAAGAGATCAAA+AGG + Chr5:89059730-89059749 None:intergenic 40.0%
TAGTCAAAGCTTGGATGCAT+GGG - Chr5:89059082-89059101 MsG0580028939.01.T01:intron 40.0%
TCATTATGTGCATCAGGTCT+TGG + Chr5:89059374-89059393 None:intergenic 40.0%
TTGTGAATGAGTTGCAGAGT+GGG + Chr5:89058915-89058934 None:intergenic 40.0%
! CCTTTTGATCTCTTTGACAG+TGG - Chr5:89059727-89059746 MsG0580028939.01.T01:intron 40.0%
! GTTTGACTTCTTTCAAGGCT+GGG - Chr5:89059583-89059602 MsG0580028939.01.T01:CDS 40.0%
! TGCAAGACTTTTACAGGTCA+TGG - Chr5:89059887-89059906 MsG0580028939.01.T01:CDS 40.0%
! TGTTTGACTTCTTTCAAGGC+TGG - Chr5:89059582-89059601 MsG0580028939.01.T01:CDS 40.0%
! TTTGAAGCTTGGGACAATTC+TGG + Chr5:89059628-89059647 None:intergenic 40.0%
AGAGACGAAAATTCTCCACG+TGG + Chr5:89059127-89059146 None:intergenic 45.0%
CACCCTTCTCTCCAAAAGAT+GGG - Chr5:89058888-89058907 MsG0580028939.01.T01:CDS 45.0%
GATACCGTGAAAGATGCTGA+CGG - Chr5:89058952-89058971 MsG0580028939.01.T01:CDS 45.0%
GTTGTGAATGAGTTGCAGAG+TGG + Chr5:89058916-89058935 None:intergenic 45.0%
TCCGCTTCATTATGTGCATC+AGG + Chr5:89059380-89059399 None:intergenic 45.0%
TGCACATAATGAAGCGGAAG+AGG - Chr5:89059382-89059401 MsG0580028939.01.T01:CDS 45.0%
TGTGAATGAGTTGCAGAGTG+GGG + Chr5:89058914-89058933 None:intergenic 45.0%
! GCAGAGTGCAAGACTTTTAC+AGG - Chr5:89059881-89059900 MsG0580028939.01.T01:CDS 45.0%
CCACCCTTCTCTCCAAAAGA+TGG - Chr5:89058887-89058906 MsG0580028939.01.T01:CDS 50.0%
GAAGCCGTCAGCATCTTTCA+CGG + Chr5:89058959-89058978 None:intergenic 50.0%
GACTTCTTTCAAGGCTGGGA+TGG - Chr5:89059587-89059606 MsG0580028939.01.T01:CDS 50.0%
GTGCGGAAAGCAAGTTTGGT+TGG + Chr5:89058868-89058887 None:intergenic 50.0%
TTCAGGCGACATCCATGGAA+AGG + Chr5:89059976-89059995 None:intergenic 50.0%
TTGCATTCAGGCGACATCCA+TGG + Chr5:89059981-89060000 None:intergenic 50.0%
!! CCATCTTTTGGAGAGAAGGG+TGG + Chr5:89058890-89058909 None:intergenic 50.0%
!! GGCCCATCTTTTGGAGAGAA+GGG + Chr5:89058893-89058912 None:intergenic 50.0%
!! TTTTGGAGAGAAGGGTGGTG+CGG + Chr5:89058885-89058904 None:intergenic 50.0%
AGGCGACATCCATGGAAAGG+AGG + Chr5:89059973-89059992 None:intergenic 55.0%
GGTGGTGCGGAAAGCAAGTT+TGG + Chr5:89058872-89058891 None:intergenic 55.0%
!! CTCCACGTGGCGTTGTTTTG+CGG + Chr5:89059114-89059133 None:intergenic 55.0%
!! GGGCCCATCTTTTGGAGAGA+AGG + Chr5:89058894-89058913 None:intergenic 55.0%
AGCCGCAAAACAACGCCACG+TGG - Chr5:89059109-89059128 MsG0580028939.01.T01:intron 60.0%
! GCAGAGTGGGGCCCATCTTT+TGG + Chr5:89058902-89058921 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 89058790 89060022 89058790 ID=MsG0580028939.01;Name=MsG0580028939.01
Chr5 mRNA 89058790 89060022 89058790 ID=MsG0580028939.01.T01;Parent=MsG0580028939.01;Name=MsG0580028939.01.T01;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|3|0|353
Chr5 exon 89059824 89060022 89059824 ID=MsG0580028939.01.T01:exon:25954;Parent=MsG0580028939.01.T01
Chr5 exon 89059148 89059734 89059148 ID=MsG0580028939.01.T01:exon:25953;Parent=MsG0580028939.01.T01
Chr5 exon 89058790 89059065 89058790 ID=MsG0580028939.01.T01:exon:25952;Parent=MsG0580028939.01.T01
Chr5 CDS 89059824 89060022 89059824 ID=MsG0580028939.01.T01:cds;Parent=MsG0580028939.01.T01
Chr5 CDS 89059148 89059734 89059148 ID=MsG0580028939.01.T01:cds;Parent=MsG0580028939.01.T01
Chr5 CDS 89058790 89059065 89058790 ID=MsG0580028939.01.T01:cds;Parent=MsG0580028939.01.T01
Gene Sequence

>MsG0580028939.01.T01

ATGGAGAACTCAACGCCCATTGATATGATCAGCAACTTATGCGATGATTTACTCTCTCATATTCTCTCTTTTCTTCCAACCAAACTTGCTTTCCGCACCACCCTTCTCTCCAAAAGATGGGCCCCACTCTGCAACTCATTCACAACTCTTCGCTTCGACGTTGATACCGTGAAAGATGCTGACGGCTTCAATCGCTTCTTTGATAGTCAAAGCTTGGATGCATGGGTAGAAGCCGCAAAACAACGCCACGTGGAGAATTTTCGTCTCTTCTTCTATAACGTAACATTGAGTCCCACAATTTTCATCTCCAAAACCCTAGTTGTTCTCAAACTTGATGGATTAAACGTTGAAACTAATAATTTGTGTGTTGATCTTCCATCTCTCAAAACCCTACATTTGCGTCAAGTTAGTTTTCACAATCGGAATGATTTCACGAAACTTCTTAATGCTTGTCCAATTCTACTAGATTTGAAAACTTCTTTTTCTACATATCCAAGACCTGATGCACATAATGAAGCGGAAGAGGTTAAATCCTTGTTCTTGTCCAAATTGGTTAGAGCCCATTTTTGTCCAAATGATATTCCGTTTAATGTAATTTCTAATGTTGAGGATCTAAGCATAGTGAATTATGGAGAGCCTTTGAAAGGCAATCCTTTCAAAGGCTTTCCTGTGTTTCAAAACTTAATTCATATTCAACTATGGTTGTTTGACTTCTTTCAAGGCTGGGATGGTGTAGTAGATTTGCTCCAGAATTGTCCCAAGCTTCAAAATCTTCGTATTAGAAAGTGGAGTTCCTGCTTATCCAATAAATGGAGATACCCAAATTCGGTTATTAAATGTGTTTCATCTTGCCTCAGATCATGTACTATTTTCAATTTTGATGGGTCAGCAGATGATCTTGCTTTCGCAACATATATTTTGCAGAGTGCAAGACTTTTACAGGTCATGGCAATCTGTACTGCTCAACCTTCAAATGAAATGCAAAAGCATCAAATTAAGCAAGATTTATCCTCCTTTCCATGGATGTCGCCTGAATGCAAACTTTCATTTGCATCTAGGTAG
Protein sequence

>MsG0580028939.01.T01

MENSTPIDMISNLCDDLLSHILSFLPTKLAFRTTLLSKRWAPLCNSFTTLRFDVDTVKDADGFNRFFDSQSLDAWVEAAKQRHVENFRLFFYNVTLSPTIFISKTLVVLKLDGLNVETNNLCVDLPSLKTLHLRQVSFHNRNDFTKLLNACPILLDLKTSFSTYPRPDAHNEAEEVKSLFLSKLVRAHFCPNDIPFNVISNVEDLSIVNYGEPLKGNPFKGFPVFQNLIHIQLWLFDFFQGWDGVVDLLQNCPKLQNLRIRKWSSCLSNKWRYPNSVIKCVSSCLRSCTIFNFDGSADDLAFATYILQSARLLQVMAICTAQPSNEMQKHQIKQDLSSFPWMSPECKLSFASR*