AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028519.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028519.01.T01 MTR_5g073420 75.556 225 43 1 1 225 73 285 3.19e-118 342
MsG0580028519.01.T01 MTR_5g061680 59.111 225 62 3 1 225 73 267 5.24e-81 245
MsG0580028519.01.T01 MTR_1g066830 53.219 233 92 5 2 225 70 294 3.33e-65 207
MsG0580028519.01.T01 MTR_5g058000 50.679 221 94 6 2 220 70 277 5.29e-60 193
MsG0580028519.01.T01 MTR_1089s0010 46.903 226 107 3 2 225 70 284 3.63e-58 188
MsG0580028519.01.T01 MTR_5g058100 47.556 225 103 6 2 220 71 286 2.48e-55 181
MsG0580028519.01.T01 MTR_6g040270 45.701 221 109 3 2 220 70 281 4.48e-52 172
MsG0580028519.01.T01 MTR_1g085120 44.444 216 107 6 2 210 241 450 2.57e-45 157
MsG0580028519.01.T01 MTR_1g085120 44.444 216 107 6 2 210 154 363 3.59e-45 157
MsG0580028519.01.T01 MTR_1g085120 44.444 216 107 6 2 210 229 438 1.04e-44 157
MsG0580028519.01.T01 MTR_5g076150 44.444 207 86 5 41 225 4 203 3.40e-43 145
MsG0580028519.01.T01 MTR_6g022500 42.035 226 114 6 2 220 163 378 5.85e-43 151
MsG0580028519.01.T01 MTR_6g011460 44.608 204 96 6 1 199 107 298 1.87e-41 146
MsG0580028519.01.T01 MTR_4g039440 41.096 219 113 9 1 210 90 301 5.86e-40 142
MsG0580028519.01.T01 MTR_7g055943 41.872 203 104 6 2 199 102 295 3.04e-35 129
MsG0580028519.01.T01 MTR_0009s0080 38.793 232 118 9 2 220 80 300 1.17e-34 128
MsG0580028519.01.T01 MTR_3g095600 45.198 177 62 6 2 175 68 212 3.74e-33 121
MsG0580028519.01.T01 MTR_6g045333 35.745 235 129 8 1 223 124 348 7.81e-33 123
MsG0580028519.01.T01 MTR_6g022360 41.262 206 102 8 2 199 92 286 2.37e-31 119
MsG0580028519.01.T01 MTR_6g022520 42.411 224 111 9 6 220 1 215 3.11e-31 117
MsG0580028519.01.T01 MTR_3g071850 34.703 219 123 6 1 208 87 296 3.42e-31 120
MsG0580028519.01.T01 MTR_5g007410 36.052 233 132 6 1 223 87 312 3.47e-31 119
MsG0580028519.01.T01 MTR_7g026500 34.874 238 126 8 2 223 76 300 4.28e-31 118
MsG0580028519.01.T01 MTR_1g108820 37.186 199 112 5 16 204 80 275 1.94e-29 113
MsG0580028519.01.T01 MTR_1g108820 37.186 199 112 5 16 204 80 275 2.74e-29 112
MsG0580028519.01.T01 MTR_5g045510 37.054 224 108 8 1 209 73 278 6.33e-29 112
MsG0580028519.01.T01 MTR_1g108800 38.974 195 106 6 13 204 48 232 2.62e-28 109
MsG0580028519.01.T01 MTR_5g058350 61.053 95 34 2 37 131 79 170 2.52e-27 103
MsG0580028519.01.T01 MTR_5g045170 35.043 234 131 6 2 223 71 295 2.99e-26 105
MsG0580028519.01.T01 MTR_3g108560 35.814 215 103 11 2 205 79 269 1.33e-23 96.7
MsG0580028519.01.T01 MTR_3g108560 35.814 215 103 11 2 205 79 269 2.64e-23 96.7
MsG0580028519.01.T01 MTR_5g039470 34.862 218 121 6 2 208 47 254 5.53e-23 95.1
MsG0580028519.01.T01 MTR_2g036470 36.986 219 114 10 1 206 75 282 2.34e-21 91.7
MsG0580028519.01.T01 MTR_5g073340 36.458 192 100 9 25 204 66 247 4.14e-21 89.7
MsG0580028519.01.T01 MTR_5g031970 32.308 195 128 3 2 193 62 255 3.72e-20 87.8
MsG0580028519.01.T01 MTR_7g007370 41.781 146 73 4 65 206 97 234 5.94e-20 87.0
MsG0580028519.01.T01 MTR_6g044572 35.714 196 93 6 29 220 119 285 6.53e-20 87.4
MsG0580028519.01.T01 MTR_5g073370 35.859 198 109 7 16 204 77 265 8.62e-20 87.0
MsG0580028519.01.T01 MTR_3g107100 35.938 192 109 7 16 199 83 268 1.53e-19 86.7
MsG0580028519.01.T01 MTR_7g056287 33.628 226 127 8 1 220 65 273 1.63e-19 86.3
MsG0580028519.01.T01 MTR_7g056277 33.962 212 118 7 1 206 321 516 1.75e-19 87.0
MsG0580028519.01.T01 MTR_6g044560 38.418 177 77 6 29 201 295 443 4.08e-19 85.9
MsG0580028519.01.T01 MTR_3g107120 33.649 211 126 6 16 220 93 295 1.48e-18 83.6
MsG0580028519.01.T01 MTR_7g088260 32.889 225 125 9 10 223 157 366 2.23e-18 83.6
MsG0580028519.01.T01 MTR_7g007340 43.046 151 65 8 56 199 102 238 2.38e-18 82.8
MsG0580028519.01.T01 MTR_3g095640 47.917 96 43 3 2 92 82 175 2.81e-18 79.3
MsG0580028519.01.T01 MTR_1g041245 34.146 205 115 9 2 199 70 261 8.11e-18 81.6
MsG0580028519.01.T01 MTR_2g007400 33.000 200 117 7 13 199 86 281 9.78e-18 81.3
MsG0580028519.01.T01 MTR_5g073400 43.478 115 57 5 13 125 4 112 1.74e-17 76.3
MsG0580028519.01.T01 MTR_0492s0020 33.684 190 110 5 10 199 152 325 2.00e-17 80.5
MsG0580028519.01.T01 MTR_0543s0010 33.684 190 110 5 10 199 152 325 2.20e-17 80.5
MsG0580028519.01.T01 MTR_3g087260 32.632 190 106 5 43 223 182 358 2.29e-16 77.8
MsG0580028519.01.T01 MTR_2g005990 32.955 176 104 5 31 197 101 271 6.70e-16 76.3
MsG0580028519.01.T01 MTR_5g039450 31.250 208 106 6 2 199 68 248 1.23e-15 75.1
MsG0580028519.01.T01 MTR_7g056447 37.903 124 74 3 10 132 75 196 2.39e-15 73.6
MsG0580028519.01.T01 MTR_5g063950 30.622 209 120 10 1 200 46 238 2.83e-15 73.9
MsG0580028519.01.T01 MTR_7g007080 30.137 219 134 9 10 220 73 280 3.16e-15 73.9
MsG0580028519.01.T01 MTR_3g095630 45.783 83 42 2 141 220 6 88 4.66e-15 71.2
MsG0580028519.01.T01 MTR_5g039490 31.937 191 99 5 1 177 81 254 4.94e-15 73.6
MsG0580028519.01.T01 MTR_3g108150 32.828 198 112 9 10 199 79 263 7.58e-15 73.2
MsG0580028519.01.T01 MTR_5g061410 33.333 168 94 4 39 199 81 237 1.03e-14 71.6
MsG0580028519.01.T01 MTR_7g098570 31.443 194 120 6 10 199 86 270 1.38e-14 72.4
MsG0580028519.01.T01 MTR_5g083890 34.239 184 103 7 38 206 110 290 1.83e-14 72.0
MsG0580028519.01.T01 MTR_5g065100 35.393 178 96 8 2 177 71 231 2.26e-14 71.6
MsG0580028519.01.T01 MTR_1g059860 35.955 178 82 7 35 207 38 188 3.05e-14 70.9
MsG0580028519.01.T01 MTR_7g098480 31.633 196 119 5 10 201 84 268 4.04e-14 71.2
MsG0580028519.01.T01 MTR_1377s0010 46.032 63 34 0 163 225 5 67 4.44e-14 67.4
MsG0580028519.01.T01 MTR_7g115270 30.000 210 128 5 10 203 78 284 5.00e-14 70.9
MsG0580028519.01.T01 MTR_2g007500 32.787 183 101 6 38 204 95 271 9.96e-14 69.3
MsG0580028519.01.T01 MTR_3g109982 39.655 116 67 2 8 123 77 189 2.16e-13 69.3
MsG0580028519.01.T01 MTR_3g109982 34.532 139 73 4 66 199 224 349 8.01e-12 64.7
MsG0580028519.01.T01 MTR_3g110010 39.655 116 67 2 8 123 77 189 2.85e-13 68.9
MsG0580028519.01.T01 MTR_3g110010 34.532 139 73 4 66 199 224 349 1.36e-11 63.9
MsG0580028519.01.T01 MTR_3g107140 38.938 113 65 2 16 125 73 184 3.64e-13 66.2
MsG0580028519.01.T01 MTR_3g099830 38.686 137 70 5 66 197 122 249 5.67e-13 67.4
MsG0580028519.01.T01 MTR_7g056020 30.660 212 114 8 8 212 73 258 5.84e-13 67.8
MsG0580028519.01.T01 MTR_7g098510 28.934 197 127 6 10 202 84 271 7.76e-13 67.0
MsG0580028519.01.T01 MTR_5g054920 36.066 122 55 3 109 220 2 110 1.15e-12 64.7
MsG0580028519.01.T01 MTR_5g033200 41.333 75 44 0 151 225 13 87 1.62e-12 63.9
MsG0580028519.01.T01 MTR_7g075820 35.664 143 79 5 62 197 142 278 1.68e-12 66.2
MsG0580028519.01.T01 MTR_4g134150 32.540 126 80 3 10 134 76 197 3.23e-12 64.3
MsG0580028519.01.T01 MTR_1g053025 30.000 200 112 7 38 221 105 292 5.00e-12 64.7
MsG0580028519.01.T01 MTR_1g107385 36.170 141 75 5 65 197 169 302 5.12e-12 64.7
MsG0580028519.01.T01 MTR_5g013500 33.113 151 83 3 10 159 61 194 6.26e-12 64.3
MsG0580028519.01.T01 MTR_7g060470 28.713 202 128 6 9 199 76 272 8.02e-12 64.3
MsG0580028519.01.T01 MTR_2g007450 27.917 240 127 11 16 220 93 321 1.04e-11 63.9
MsG0580028519.01.T01 MTR_7g056430 29.952 207 107 8 10 209 75 250 1.28e-11 63.9
MsG0580028519.01.T01 MTR_7g056420 29.952 207 107 8 10 209 75 250 1.36e-11 63.9
MsG0580028519.01.T01 MTR_6g083130 40.206 97 45 2 29 125 226 309 2.20e-11 62.8
MsG0580028519.01.T01 MTR_7g034120 30.049 203 115 9 10 199 4 192 2.71e-11 62.4
MsG0580028519.01.T01 MTR_2g008310 34.043 141 84 5 65 199 122 259 3.35e-11 62.4
MsG0580028519.01.T01 MTR_2g008320 34.228 149 73 6 65 199 176 313 4.10e-11 62.4
MsG0580028519.01.T01 MTR_3g107160 31.429 175 101 7 35 200 105 269 5.25e-11 62.0
MsG0580028519.01.T01 MTR_7g034115 28.070 228 141 10 2 220 70 283 6.73e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028519.01.T01 AT5G22660 33.028 109 67 3 38 143 104 209 2.67e-11 62.4
MsG0580028519.01.T01 AT5G22660 33.028 109 67 3 38 143 104 209 6.53e-11 61.6

Find 34 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAGTACAAGAATGATTTA+AGG 0.188423 5:+81755899 MsG0580028519.01.T01:CDS
GATTTAAGGAAACTTCTTAA+TGG 0.262391 5:+81755913 MsG0580028519.01.T01:CDS
ACTTAATTCACATTAAACTA+TGG 0.293230 5:+81756166 MsG0580028519.01.T01:CDS
CTTGAAGCTTTGGGCAATGC+TGG 0.352982 5:-81756234 None:intergenic
GTTTCAGAACAACTAGGGTT+TGG 0.360325 5:-81755795 None:intergenic
TTTCAGAACAACTAGGGTTT+GGG 0.373808 5:-81755794 None:intergenic
TGCTCTAACCAACTTTGAAA+AGG 0.383090 5:-81756037 None:intergenic
TGGTTGCATGACTTTATTGA+TGG 0.400423 5:+81756186 MsG0580028519.01.T01:CDS
GTTTGGGAGGTGAAAATTGT+GGG 0.416123 5:-81755778 None:intergenic
AACAGATTTCTTTCAAATGT+AGG 0.424636 5:-81755876 None:intergenic
GTATGTAAATCTTCTAGAAC+TGG 0.435027 5:-81755940 None:intergenic
TGCATGACTTTATTGATGGC+TGG 0.443969 5:+81756190 MsG0580028519.01.T01:CDS
AGATTATTAATTGCATCAAA+AGG 0.457631 5:-81756081 None:intergenic
GCTCTAACCAACTTTGAAAA+GGG 0.462352 5:-81756036 None:intergenic
TGCAGTCATGTGTTCTCCTC+GGG 0.476557 5:+81755597 MsG0580028519.01.T01:CDS
TTCATTATTGTGAGAAAGGT+AGG 0.482675 5:+81755662 MsG0580028519.01.T01:CDS
GACTTTATTGATGGCTGGAA+TGG 0.484192 5:+81756195 MsG0580028519.01.T01:CDS
CTCAAGTTTCAGAACAACTA+GGG 0.498410 5:-81755800 None:intergenic
CGCGACACAGCGCCGAGTTG+AGG 0.510117 5:+81755711 MsG0580028519.01.T01:CDS
TCTCAAGTTTCAGAACAACT+AGG 0.513803 5:-81755801 None:intergenic
ATGCAGTCATGTGTTCTCCT+CGG 0.523204 5:+81755596 MsG0580028519.01.T01:CDS
CTCAACTCGGCGCTGTGTCG+CGG 0.532818 5:-81755710 None:intergenic
TTTATTGATGGCTGGAATGG+TGG 0.563639 5:+81756198 MsG0580028519.01.T01:CDS
ATTGATGGCTGGAATGGTGG+TGG 0.565871 5:+81756201 MsG0580028519.01.T01:CDS
GAAGAAAATAATGCAGCGGA+AGG 0.572516 5:+81756006 MsG0580028519.01.T01:CDS
CAGAACAACTAGGGTTTGGG+AGG 0.591012 5:-81755791 None:intergenic
GGTTTGGGAGGTGAAAATTG+TGG 0.591857 5:-81755779 None:intergenic
TGAGTTTCTTTGCATACGCG+TGG 0.602883 5:+81756104 MsG0580028519.01.T01:CDS
ATGGGTTGGTTTATTTCCCG+AGG 0.607650 5:-81755613 None:intergenic
AAGAAAATAATGCAGCGGAA+GGG 0.629433 5:+81756007 MsG0580028519.01.T01:CDS
TTTAATCTCTCGATAGTCAG+TGG 0.652814 5:+81755736 MsG0580028519.01.T01:CDS
AGTTGAAGAAAATAATGCAG+CGG 0.658593 5:+81756002 MsG0580028519.01.T01:CDS
AATTGTGGGATTCAACATGA+AGG 0.662017 5:-81755764 None:intergenic
AGAGATTAAATTCCTCAACT+CGG 0.663070 5:-81755723 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAATTCACATTAAACTA+TGG + Chr5:81756166-81756185 MsG0580028519.01.T01:CDS 20.0%
!! AGATTATTAATTGCATCAAA+AGG - Chr5:81756084-81756103 None:intergenic 20.0%
!! TTAAGTACAAGAATGATTTA+AGG + Chr5:81755899-81755918 MsG0580028519.01.T01:CDS 20.0%
!!! TGAATTAAGTTTTGAAACAA+TGG - Chr5:81756156-81756175 None:intergenic 20.0%
! AACAGATTTCTTTCAAATGT+AGG - Chr5:81755879-81755898 None:intergenic 25.0%
! GATTTAAGGAAACTTCTTAA+TGG + Chr5:81755913-81755932 MsG0580028519.01.T01:CDS 25.0%
!! AGTTTTCATTATTGTGAGAA+AGG + Chr5:81755658-81755677 MsG0580028519.01.T01:CDS 25.0%
!!! TAAGTTTTGAAACAATGGAA+TGG - Chr5:81756151-81756170 None:intergenic 25.0%
!!! TTGATATGAAACGTTTTGAT+GGG - Chr5:81755634-81755653 None:intergenic 25.0%
AGAGATTAAATTCCTCAACT+CGG - Chr5:81755726-81755745 None:intergenic 30.0%
AGTTGAAGAAAATAATGCAG+CGG + Chr5:81756002-81756021 MsG0580028519.01.T01:CDS 30.0%
GAAAAAGAACTTGAAGCTTT+GGG - Chr5:81756246-81756265 None:intergenic 30.0%
TGAAAAAGAACTTGAAGCTT+TGG - Chr5:81756247-81756266 None:intergenic 30.0%
TTCATTATTGTGAGAAAGGT+AGG + Chr5:81755662-81755681 MsG0580028519.01.T01:CDS 30.0%
! GTTGATATGAAACGTTTTGA+TGG - Chr5:81755635-81755654 None:intergenic 30.0%
! GTTTAAACCCTTTTCAAAGT+TGG + Chr5:81756029-81756048 MsG0580028519.01.T01:CDS 30.0%
!! AAATGTAGGATTTTGAGAGA+TGG - Chr5:81755865-81755884 None:intergenic 30.0%
!! GTATGTAAATCTTCTAGAAC+TGG - Chr5:81755943-81755962 None:intergenic 30.0%
AAGAAAATAATGCAGCGGAA+GGG + Chr5:81756007-81756026 MsG0580028519.01.T01:CDS 35.0%
AATTGTGGGATTCAACATGA+AGG - Chr5:81755767-81755786 None:intergenic 35.0%
CTCAAGTTTCAGAACAACTA+GGG - Chr5:81755803-81755822 None:intergenic 35.0%
TCTCAAGTTTCAGAACAACT+AGG - Chr5:81755804-81755823 None:intergenic 35.0%
TGGTTGCATGACTTTATTGA+TGG + Chr5:81756186-81756205 MsG0580028519.01.T01:CDS 35.0%
TTTAATCTCTCGATAGTCAG+TGG + Chr5:81755736-81755755 MsG0580028519.01.T01:CDS 35.0%
TTTCAGAACAACTAGGGTTT+GGG - Chr5:81755797-81755816 None:intergenic 35.0%
! GCTCTAACCAACTTTGAAAA+GGG - Chr5:81756039-81756058 None:intergenic 35.0%
! TAGGACTATTTTCGATGCAT+GGG + Chr5:81755681-81755700 MsG0580028519.01.T01:CDS 35.0%
! TGCTCTAACCAACTTTGAAA+AGG - Chr5:81756040-81756059 None:intergenic 35.0%
!! TATGAAACGTTTTGATGGGT+TGG - Chr5:81755630-81755649 None:intergenic 35.0%
GAAGAAAATAATGCAGCGGA+AGG + Chr5:81756006-81756025 MsG0580028519.01.T01:CDS 40.0%
GTTTCAGAACAACTAGGGTT+TGG - Chr5:81755798-81755817 None:intergenic 40.0%
GTTTGGGAGGTGAAAATTGT+GGG - Chr5:81755781-81755800 None:intergenic 40.0%
! GACTTTATTGATGGCTGGAA+TGG + Chr5:81756195-81756214 MsG0580028519.01.T01:CDS 40.0%
! GTAGGACTATTTTCGATGCA+TGG + Chr5:81755680-81755699 MsG0580028519.01.T01:CDS 40.0%
! TGCATGACTTTATTGATGGC+TGG + Chr5:81756190-81756209 MsG0580028519.01.T01:CDS 40.0%
! TTTATTGATGGCTGGAATGG+TGG + Chr5:81756198-81756217 MsG0580028519.01.T01:CDS 40.0%
ATGCAGTCATGTGTTCTCCT+CGG + Chr5:81755596-81755615 MsG0580028519.01.T01:CDS 45.0%
GGTTTGGGAGGTGAAAATTG+TGG - Chr5:81755782-81755801 None:intergenic 45.0%
TGAGTTTCTTTGCATACGCG+TGG + Chr5:81756104-81756123 MsG0580028519.01.T01:CDS 45.0%
!! ATGGGTTGGTTTATTTCCCG+AGG - Chr5:81755616-81755635 None:intergenic 45.0%
CAGAACAACTAGGGTTTGGG+AGG - Chr5:81755794-81755813 None:intergenic 50.0%
TGCAGTCATGTGTTCTCCTC+GGG + Chr5:81755597-81755616 MsG0580028519.01.T01:CDS 50.0%
! ATTGATGGCTGGAATGGTGG+TGG + Chr5:81756201-81756220 MsG0580028519.01.T01:CDS 50.0%
!! CTTGAAGCTTTGGGCAATGC+TGG - Chr5:81756237-81756256 None:intergenic 50.0%
CTCAACTCGGCGCTGTGTCG+CGG - Chr5:81755713-81755732 None:intergenic 65.0%
! CGCGACACAGCGCCGAGTTG+AGG + Chr5:81755711-81755730 MsG0580028519.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 81755589 81756269 81755589 ID=MsG0580028519.01;Name=MsG0580028519.01
Chr5 mRNA 81755589 81756269 81755589 ID=MsG0580028519.01.T01;Parent=MsG0580028519.01;Name=MsG0580028519.01.T01;_AED=0.47;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|226
Chr5 exon 81755589 81756269 81755589 ID=MsG0580028519.01.T01:exon:32976;Parent=MsG0580028519.01.T01
Chr5 CDS 81755589 81756269 81755589 ID=MsG0580028519.01.T01:cds;Parent=MsG0580028519.01.T01
Gene Sequence

>MsG0580028519.01.T01

ATGGTGGATGCAGTCATGTGTTCTCCTCGGGAAATAAACCAACCCATCAAAACGTTTCATATCAACTGTAGTTTTCATTATTGTGAGAAAGGTAGGACTATTTTCGATGCATGGGTAGAAGCCGCGACACAGCGCCGAGTTGAGGAATTTAATCTCTCGATAGTCAGTGGTATCACCTTCATGTTGAATCCCACAATTTTCACCTCCCAAACCCTAGTTGTTCTGAAACTTGAGAGATTCGTAGTTAAAGCTGAAAATTTGTGTGTTGATCTTCCATCTCTCAAAATCCTACATTTGAAAGAAATCTGTTTTAAGTACAAGAATGATTTAAGGAAACTTCTTAATGGTTGTCCAGTTCTAGAAGATTTACATACTTATCGTCGTACATATAGTTATCGTCGTACATATATGAGAGTTGAAGAAAATAATGCAGCGGAAGGGTTTAAACCCTTTTCAAAGTTGGTTAGAGCAGATATCAATTCAAATGATGTTCCTTTTGATGCAATTAATAATCTTGAGTTTCTTTGCATACGCGTGGCACGAGAAAAATCTTACAAAACCATTCCATTGTTTCAAAACTTAATTCACATTAAACTATGGTTGCATGACTTTATTGATGGCTGGAATGGTGGTGGTAGAGTTTCTCCAGCATTGCCCAAAGCTTCAAGTTCTTTTTCATGA
Protein sequence

>MsG0580028519.01.T01

MVDAVMCSPREINQPIKTFHINCSFHYCEKGRTIFDAWVEAATQRRVEEFNLSIVSGITFMLNPTIFTSQTLVVLKLERFVVKAENLCVDLPSLKILHLKEICFKYKNDLRKLLNGCPVLEDLHTYRRTYSYRRTYMRVEENNAAEGFKPFSKLVRADINSNDVPFDAINNLEFLCIRVAREKSYKTIPLFQNLIHIKLWLHDFIDGWNGGGRVSPALPKASSSFS*