AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024185.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024185.01.T01 MTR_5g006960 100.000 161 0 0 27 187 238 398 4.89e-115 332
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024185.01.T01 AT3G22590 90.184 163 14 1 27 187 253 415 1.28e-104 306

Find 53 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAGTATATTGTTAAATT+AGG 0.131153 5:-1711084 MsG0580024185.01.T01:three_prime_UTR
TATCCGAATATTGCAATATT+TGG 0.151297 5:-1711161 MsG0580024185.01.T01:three_prime_UTR
CAATATACTACATTGTTAAA+AGG 0.224048 5:+1711094 None:intergenic
GCGAAGGTTTCCGATTATTT+TGG 0.248133 5:-1711909 MsG0580024185.01.T01:CDS
CTGTTACTGCTTATGAAGTT+AGG 0.278775 5:-1711733 MsG0580024185.01.T01:CDS
ATGGGAAGTGCTGATGATTT+TGG 0.292521 5:-1711990 MsG0580024188.01.T01:intron
ATCTCGACATGATCCTTAAA+AGG 0.296624 5:+1711623 None:intergenic
TGTAGTATATTGTTAAATTA+GGG 0.298869 5:-1711083 MsG0580024185.01.T01:three_prime_UTR
GCATGAAAATTAATCTTAGA+TGG 0.367738 5:+1710925 None:intergenic
TTATGTACCTACTGATGTTA+AGG 0.369770 5:-1711822 MsG0580024185.01.T01:CDS
TATTACTATCTATAATGTTA+AGG 0.376267 5:-1711864 MsG0580024185.01.T01:CDS
CTGTTCAGAAGAAGCTTAGT+AGG 0.378789 5:-1711763 MsG0580024185.01.T01:CDS
ACACCAAATATTGCAATATT+CGG 0.379609 5:+1711158 None:intergenic
TCAAGACAGGGCTGCGGCAT+TGG 0.380453 5:-1711291 MsG0580024185.01.T01:CDS
TACAGTGATCGCTAAAAGTA+TGG 0.393026 5:+1711209 None:intergenic
TGCGCTTAAACCTGAGGATT+GGG 0.400010 5:-1711699 MsG0580024185.01.T01:CDS
ACCTAAAATGCATCTTAAAT+CGG 0.412984 5:-1711931 MsG0580024185.01.T01:CDS
ATATGCTATTTCACCGTGTA+AGG 0.419044 5:-1711021 MsG0580024185.01.T01:three_prime_UTR
GCACTCGGAACCAAAATAAT+CGG 0.435108 5:+1711899 None:intergenic
GGGAAAGGATTGGCAGTTCA+AGG 0.438397 5:-1711651 MsG0580024185.01.T01:CDS
GGCTGCGGCATTGGAGGTGT+GGG 0.440734 5:-1711282 MsG0580024185.01.T01:CDS
GGTGTCTTGTAATGTTATAT+CGG 0.449460 5:-1711140 MsG0580024185.01.T01:three_prime_UTR
GGGCTGCGGCATTGGAGGTG+TGG 0.449671 5:-1711283 MsG0580024185.01.T01:CDS
TGTTACTGCTTATGAAGTTA+GGG 0.450144 5:-1711732 MsG0580024185.01.T01:CDS
CTGCGCTTAAACCTGAGGAT+TGG 0.455759 5:-1711700 MsG0580024185.01.T01:CDS
ACCTACTGATGTTAAGGTTA+AGG 0.458526 5:-1711816 MsG0580024185.01.T01:CDS
AGAACAAGCGGCATCAAGAC+AGG 0.461687 5:-1711304 MsG0580024185.01.T01:CDS
TTCTGAACAGTAACACAATC+CGG 0.474450 5:+1711776 None:intergenic
GGCGATGAAAGGTGCTAAGC+CGG 0.488489 5:-1711795 MsG0580024185.01.T01:CDS
AGACAGGGCTGCGGCATTGG+AGG 0.490687 5:-1711288 MsG0580024185.01.T01:CDS
ATTGGAGGTGTGGGACAGAC+TGG 0.497768 5:-1711273 MsG0580024185.01.T01:CDS
ATCCATCTTTAGCACACAAA+AGG 0.498482 5:+1711042 None:intergenic
AGGATTGGCAGTTCAAGGAT+TGG 0.523053 5:-1711646 MsG0580024185.01.T01:CDS
TCCTCAGGTTTAAGCGCAGA+AGG 0.543271 5:+1711704 None:intergenic
TATTGCAATATTCGGATACA+AGG 0.546360 5:+1711166 None:intergenic
TGTTCAGAAGAAGCTTAGTA+GGG 0.562083 5:-1711762 MsG0580024185.01.T01:CDS
CTATTTCACCGTGTAAGGGA+TGG 0.566604 5:-1711016 MsG0580024185.01.T01:three_prime_UTR
GCCTTCTGCGCTTAAACCTG+AGG 0.566756 5:-1711705 MsG0580024185.01.T01:CDS
ACAACACGATCCCAATCCTC+AGG 0.568851 5:+1711689 None:intergenic
CACAAAAGGATATTAGTGAC+AGG 0.571107 5:+1711056 None:intergenic
AATGCATCTTAAATCGGCGA+AGG 0.577375 5:-1711925 MsG0580024185.01.T01:CDS
TATACTACATTGTTAAAAGG+TGG 0.584796 5:+1711097 None:intergenic
GCGGCATCAAGACAGGGCTG+CGG 0.587480 5:-1711297 MsG0580024185.01.T01:CDS
GTTAAGGTTAAGGCGATGAA+AGG 0.589838 5:-1711806 MsG0580024185.01.T01:CDS
TTCAGATTAGTAAGAACAAG+CGG 0.592064 5:-1711316 MsG0580024185.01.T01:intron
CTGCGAAGACTGTGAAGCAG+TGG 0.602270 5:-1711446 MsG0580024185.01.T01:CDS
TATGCTATTTCACCGTGTAA+GGG 0.618252 5:-1711020 MsG0580024185.01.T01:three_prime_UTR
GTGGAATGTGAAGATTATCT+CGG 0.629342 5:-1711427 MsG0580024185.01.T01:intron
GCCTTAACCTTAACATCAGT+AGG 0.634280 5:+1711815 None:intergenic
ATAAGTGTCTGAAAAGCACT+CGG 0.638006 5:+1711884 None:intergenic
GAACAAGCGGCATCAAGACA+GGG 0.639576 5:-1711303 MsG0580024185.01.T01:CDS
AGTGATCGCTAAAAGTATGG+TGG 0.690555 5:+1711212 None:intergenic
GGATTGGGATCGTGTTGTCG+CGG 0.703493 5:-1711684 MsG0580024185.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAACAATCAAAATAAAA+TGG + Chr5:1711316-1711335 None:intergenic 10.0%
!! ATGTAGTATATTGTTAAATT+AGG - Chr5:1711804-1711823 MsG0580024185.01.T01:CDS 15.0%
!! TATTACTATCTATAATGTTA+AGG - Chr5:1711024-1711043 MsG0580024185.01.T01:three_prime_UTR 15.0%
!! TGTAGTATATTGTTAAATTA+GGG - Chr5:1711805-1711824 MsG0580024185.01.T01:CDS 15.0%
!! CAATATACTACATTGTTAAA+AGG + Chr5:1711797-1711816 None:intergenic 20.0%
!!! CTATAATGTTAAGGATTTTT+TGG - Chr5:1711033-1711052 MsG0580024185.01.T01:three_prime_UTR 20.0%
! ACACCAAATATTGCAATATT+CGG + Chr5:1711733-1711752 None:intergenic 25.0%
! ACCTAAAATGCATCTTAAAT+CGG - Chr5:1710957-1710976 MsG0580024185.01.T01:three_prime_UTR 25.0%
! GCATGAAAATTAATCTTAGA+TGG + Chr5:1711966-1711985 None:intergenic 25.0%
! TATACTACATTGTTAAAAGG+TGG + Chr5:1711794-1711813 None:intergenic 25.0%
! TATCCGAATATTGCAATATT+TGG - Chr5:1711727-1711746 MsG0580024185.01.T01:CDS 25.0%
!! GATTAATTTTCATGCACATT+TGG - Chr5:1711971-1711990 MsG0580024185.01.T01:CDS 25.0%
!! TCGAGATTTTCAATAAAAGT+CGG - Chr5:1711281-1711300 MsG0580024185.01.T01:CDS 25.0%
!!! TTAAGATGCATTTTAGGTTT+CGG + Chr5:1710955-1710974 None:intergenic 25.0%
!!! TTAAGGATTTTTTGGAAGAT+GGG - Chr5:1711041-1711060 MsG0580024185.01.T01:three_prime_UTR 25.0%
GGTGTCTTGTAATGTTATAT+CGG - Chr5:1711748-1711767 MsG0580024185.01.T01:CDS 30.0%
TATTGCAATATTCGGATACA+AGG + Chr5:1711725-1711744 None:intergenic 30.0%
TGTTACTGCTTATGAAGTTA+GGG - Chr5:1711156-1711175 MsG0580024185.01.T01:three_prime_UTR 30.0%
TTATGTACCTACTGATGTTA+AGG - Chr5:1711066-1711085 MsG0580024185.01.T01:three_prime_UTR 30.0%
TTCAGATTAGTAAGAACAAG+CGG - Chr5:1711572-1711591 MsG0580024185.01.T01:intron 30.0%
!!! CAGTTACTGGATTTTTTATG+CGG - Chr5:1711397-1711416 MsG0580024185.01.T01:intron 30.0%
!!! GTTAAGGATTTTTTGGAAGA+TGG - Chr5:1711040-1711059 MsG0580024185.01.T01:three_prime_UTR 30.0%
!!! TAAGGATTTTTTGGAAGATG+GGG - Chr5:1711042-1711061 MsG0580024185.01.T01:three_prime_UTR 30.0%
!!! TTTTTGTTTTGGGAAAGGAT+TGG - Chr5:1711227-1711246 MsG0580024185.01.T01:exon 30.0%
AAAAACTTCCATCCCTTACA+CGG + Chr5:1711883-1711902 None:intergenic 35.0%
ACCTACTGATGTTAAGGTTA+AGG - Chr5:1711072-1711091 MsG0580024185.01.T01:three_prime_UTR 35.0%
AGTAACTGCAACAATTTGAC+AGG + Chr5:1711385-1711404 None:intergenic 35.0%
ATCCATCTTTAGCACACAAA+AGG + Chr5:1711849-1711868 None:intergenic 35.0%
ATCTCGACATGATCCTTAAA+AGG + Chr5:1711268-1711287 None:intergenic 35.0%
CACAAAAGGATATTAGTGAC+AGG + Chr5:1711835-1711854 None:intergenic 35.0%
CTGTTACTGCTTATGAAGTT+AGG - Chr5:1711155-1711174 MsG0580024185.01.T01:three_prime_UTR 35.0%
GTAACTGCAACAATTTGACA+GGG + Chr5:1711384-1711403 None:intergenic 35.0%
GTGCAGTGTAAGTTAAAAAC+TGG + Chr5:1711535-1711554 None:intergenic 35.0%
GTGGAATGTGAAGATTATCT+CGG - Chr5:1711461-1711480 MsG0580024185.01.T01:CDS 35.0%
TAACTGCAACAATTTGACAG+GGG + Chr5:1711383-1711402 None:intergenic 35.0%
TACAGTGATCGCTAAAAGTA+TGG + Chr5:1711682-1711701 None:intergenic 35.0%
TGTCAAATTGTTGCAGTTAC+TGG - Chr5:1711384-1711403 MsG0580024185.01.T01:intron 35.0%
TGTTCAGAAGAAGCTTAGTA+GGG - Chr5:1711126-1711145 MsG0580024185.01.T01:three_prime_UTR 35.0%
TTCTGAACAGTAACACAATC+CGG + Chr5:1711115-1711134 None:intergenic 35.0%
! ATCCTTTTGTGTGCTAAAGA+TGG - Chr5:1711844-1711863 MsG0580024185.01.T01:CDS 35.0%
!! ATAAGTGTCTGAAAAGCACT+CGG + Chr5:1711007-1711026 None:intergenic 35.0%
!! ATATGCTATTTCACCGTGTA+AGG - Chr5:1711867-1711886 MsG0580024185.01.T01:CDS 35.0%
!! GCCGATTTAAGATGCATTTT+AGG + Chr5:1710961-1710980 None:intergenic 35.0%
!! TATGCTATTTCACCGTGTAA+GGG - Chr5:1711868-1711887 MsG0580024185.01.T01:CDS 35.0%
!!! ATTTTGGTGGTGATGAAATG+GGG - Chr5:1710914-1710933 MsG0580024185.01.T01:three_prime_UTR 35.0%
!!! GATTTTGGTGGTGATGAAAT+GGG - Chr5:1710913-1710932 MsG0580024185.01.T01:three_prime_UTR 35.0%
!!! TGATTTTGGTGGTGATGAAA+TGG - Chr5:1710912-1710931 MsG0580024185.01.T01:three_prime_UTR 35.0%
AATGCATCTTAAATCGGCGA+AGG - Chr5:1710963-1710982 MsG0580024185.01.T01:three_prime_UTR 40.0%
CTGTTCAGAAGAAGCTTAGT+AGG - Chr5:1711125-1711144 MsG0580024185.01.T01:three_prime_UTR 40.0%
GCACTCGGAACCAAAATAAT+CGG + Chr5:1710992-1711011 None:intergenic 40.0%
GCATAGCAGTAGTTAGAACA+AGG + Chr5:1711497-1711516 None:intergenic 40.0%
GCCTTAACCTTAACATCAGT+AGG + Chr5:1711076-1711095 None:intergenic 40.0%
GTTAAGGTTAAGGCGATGAA+AGG - Chr5:1711082-1711101 MsG0580024185.01.T01:three_prime_UTR 40.0%
! AGTGATCGCTAAAAGTATGG+TGG + Chr5:1711679-1711698 None:intergenic 40.0%
! GCGAAGGTTTCCGATTATTT+TGG - Chr5:1710979-1710998 MsG0580024185.01.T01:three_prime_UTR 40.0%
!! TGTTGTCGCGGTTTTTGTTT+TGG - Chr5:1711216-1711235 MsG0580024185.01.T01:three_prime_UTR 40.0%
!!! GTTCAAGGATTGGCCTTTTA+AGG - Chr5:1711252-1711271 MsG0580024185.01.T01:CDS 40.0%
!!! GTTGTCGCGGTTTTTGTTTT+GGG - Chr5:1711217-1711236 MsG0580024185.01.T01:three_prime_UTR 40.0%
CTATTTCACCGTGTAAGGGA+TGG - Chr5:1711872-1711891 MsG0580024185.01.T01:CDS 45.0%
TGCGCTTAAACCTGAGGATT+GGG - Chr5:1711189-1711208 MsG0580024185.01.T01:three_prime_UTR 45.0%
! AGGATTGGCAGTTCAAGGAT+TGG - Chr5:1711242-1711261 MsG0580024185.01.T01:CDS 45.0%
!!! CGCGGTTTTTGTTTTGGGAA+AGG - Chr5:1711222-1711241 MsG0580024185.01.T01:exon 45.0%
ACAACACGATCCCAATCCTC+AGG + Chr5:1711202-1711221 None:intergenic 50.0%
AGAACAAGCGGCATCAAGAC+AGG - Chr5:1711584-1711603 MsG0580024185.01.T01:intron 50.0%
CTGCGCTTAAACCTGAGGAT+TGG - Chr5:1711188-1711207 MsG0580024185.01.T01:three_prime_UTR 50.0%
GAACAAGCGGCATCAAGACA+GGG - Chr5:1711585-1711604 MsG0580024185.01.T01:intron 50.0%
TCCTCAGGTTTAAGCGCAGA+AGG + Chr5:1711187-1711206 None:intergenic 50.0%
! GGGAAAGGATTGGCAGTTCA+AGG - Chr5:1711237-1711256 MsG0580024185.01.T01:exon 50.0%
CTGCGAAGACTGTGAAGCAG+TGG - Chr5:1711442-1711461 MsG0580024185.01.T01:CDS 55.0%
GCCTTCTGCGCTTAAACCTG+AGG - Chr5:1711183-1711202 MsG0580024185.01.T01:three_prime_UTR 55.0%
GGATTGGGATCGTGTTGTCG+CGG - Chr5:1711204-1711223 MsG0580024185.01.T01:three_prime_UTR 55.0%
! ATTGGAGGTGTGGGACAGAC+TGG - Chr5:1711615-1711634 MsG0580024185.01.T01:CDS 55.0%
!! GGCGATGAAAGGTGCTAAGC+CGG - Chr5:1711093-1711112 MsG0580024185.01.T01:three_prime_UTR 55.0%
TCAAGACAGGGCTGCGGCAT+TGG - Chr5:1711597-1711616 MsG0580024185.01.T01:intron 60.0%
GCGGCATCAAGACAGGGCTG+CGG - Chr5:1711591-1711610 MsG0580024185.01.T01:intron 65.0%
! AGACAGGGCTGCGGCATTGG+AGG - Chr5:1711600-1711619 MsG0580024185.01.T01:intron 65.0%
! GGCTGCGGCATTGGAGGTGT+GGG - Chr5:1711606-1711625 MsG0580024185.01.T01:intron 65.0%
! GGGCTGCGGCATTGGAGGTG+TGG - Chr5:1711605-1711624 MsG0580024185.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 1710911 1711999 1710911 ID=MsG0580024185.01;Name=MsG0580024185.01
Chr5 mRNA 1710911 1711999 1710911 ID=MsG0580024185.01.T01;Parent=MsG0580024185.01;Name=MsG0580024185.01.T01;_AED=0.29;_eAED=0.29;_QI=0|1|1|1|1|1|3|330|187
Chr5 exon 1710911 1711333 1710911 ID=MsG0580024185.01.T01:exon:490;Parent=MsG0580024185.01.T01
Chr5 exon 1711428 1711510 1711428 ID=MsG0580024185.01.T01:exon:489;Parent=MsG0580024185.01.T01
Chr5 exon 1711612 1711999 1711612 ID=MsG0580024185.01.T01:exon:488;Parent=MsG0580024185.01.T01
Chr5 CDS 1711612 1711999 1711612 ID=MsG0580024185.01.T01:cds;Parent=MsG0580024185.01.T01
Chr5 CDS 1711428 1711510 1711428 ID=MsG0580024185.01.T01:cds;Parent=MsG0580024185.01.T01
Chr5 CDS 1711241 1711333 1711241 ID=MsG0580024185.01.T01:cds;Parent=MsG0580024185.01.T01
Chr5 three_prime_UTR 1710911 1711240 1710911 ID=MsG0580024185.01.T01:three_prime_utr;Parent=MsG0580024185.01.T01
Gene Sequence

>MsG0580024185.01.T01

ATGATTTTGGTGGTGATGAAATGGGGTATGATCATCAAACACCGAAACCTAAAATGCATCTTAAATCGGCGAAGGTTTCCGATTATTTTGGTTCCGAGTGCTTTTCAGACACTTATTACTATCTATAATGTTAAGGATTTTTTGGAAGATGGGGTTTATGTACCTACTGATGTTAAGGTTAAGGCGATGAAAGGTGCTAAGCCGGATTGTGTTACTGTTCAGAAGAAGCTTAGTAGGGATAGAGCTGTTACTGCTTATGAAGTTAGGGATAAGCCTTCTGCGCTTAAACCTGAGGATTGGGATCGTGTTGTCGCGGTTTTTGTTTTGGGAAAGGATTGGCAGTTCAAGGATTGGCCTTTTAAGGATCATGTCGAGATTTTCAATAAAATTACTGGATTTTTTATGCGGTTTGAAGATGATAGTATAGAGTCTGCGAAGACTGTGAAGCAGTGGAATGTGAAGATTATCTCGATTAGTAAGAACAAGCGGCATCAAGACAGGGCTGCGGCATTGGAGGTGTGGGACAGACTGGAAGAATTTGTGCGCTCACGATCACATTCTTGA

Protein sequence

>MsG0580024185.01.T01

MILVVMKWGMIIKHRNLKCILNRRRFPIILVPSAFQTLITIYNVKDFLEDGVYVPTDVKVKAMKGAKPDCVTVQKKLSRDRAVTAYEVRDKPSALKPEDWDRVVAVFVLGKDWQFKDWPFKDHVEIFNKITGFFMRFEDDSIESAKTVKQWNVKIISISKNKRHQDRAAALEVWDRLEEFVRSRSHS*