AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039903.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATGTGGTTGATGGTATC+TGG 0.150412 7:+70279248 MsG0780039903.01.T01:CDS
GGAGAGGGAGGTAGGGGAGA+TGG 0.331242 7:+70279136 MsG0780039903.01.T01:CDS
GAGGGAGGTAGGGGAGATGG+AGG 0.337923 7:+70279139 MsG0780039903.01.T01:CDS
AGATGGAGGTTGTGAAAAGG+TGG 0.364567 7:+70279153 MsG0780039903.01.T01:CDS
GGGAGAATGGTGGACTAGCC+GGG 0.384778 7:+70279175 MsG0780039903.01.T01:CDS
GGGGAGAATGGTGGACTAGC+CGG 0.402579 7:+70279174 MsG0780039903.01.T01:CDS
ACGTCGGTGAAGAGGAGGTT+AGG 0.409537 7:+70279041 MsG0780039903.01.T01:CDS
TGGTTGATGGTATCTGGGTG+AGG 0.426999 7:+70279254 MsG0780039903.01.T01:CDS
TTGTGAAAAGGTGGGGAGAA+TGG 0.428753 7:+70279162 MsG0780039903.01.T01:CDS
GATGGTGAGGATGTGGTTGA+TGG 0.436022 7:+70279241 MsG0780039903.01.T01:CDS
GGAGAATGGTGGACTAGCCG+GGG 0.455928 7:+70279176 MsG0780039903.01.T01:CDS
GAAGTGAGGAAGGTGAAGAC+GGG 0.460673 7:+70279113 MsG0780039903.01.T01:CDS
GGATGTGGTTGATGGTATCT+GGG 0.481384 7:+70279249 MsG0780039903.01.T01:CDS
GGGGATGTCTGCCTCGGTGA+AGG 0.482904 7:+70279196 MsG0780039903.01.T01:CDS
CGGTGAAGGAGTGGGATTTG+AGG 0.484295 7:+70279210 MsG0780039903.01.T01:CDS
GGTTAGGGCAACGAAGGATA+AGG 0.486468 7:+70279057 MsG0780039903.01.T01:CDS
GGGAGATGGAGGTTGTGAAA+AGG 0.502247 7:+70279150 MsG0780039903.01.T01:CDS
GAGAATGGTGGACTAGCCGG+GGG 0.511187 7:+70279177 MsG0780039903.01.T01:CDS
GGAAGTGAGGAAGGTGAAGA+CGG 0.514910 7:+70279112 MsG0780039903.01.T01:CDS
TCACCGAGGCAGACATCCCC+CGG 0.522004 7:-70279193 None:intergenic
GTCTGCCTCGGTGAAGGAGT+GGG 0.530680 7:+70279202 MsG0780039903.01.T01:CDS
CGTCGGTGAAGAGGAGGTTA+GGG 0.540321 7:+70279042 MsG0780039903.01.T01:CDS
TGAAAAGGTGGGGAGAATGG+TGG 0.545799 7:+70279165 MsG0780039903.01.T01:CDS
GATGGAGGTTGTGAAAAGGT+GGG 0.554116 7:+70279154 MsG0780039903.01.T01:CDS
GGTTGATGGTATCTGGGTGA+GGG 0.557111 7:+70279255 MsG0780039903.01.T01:CDS
TGTCTGCCTCGGTGAAGGAG+TGG 0.557235 7:+70279201 MsG0780039903.01.T01:CDS
GAGGAGGTCGGAAGTGAGGA+AGG 0.563733 7:+70279103 MsG0780039903.01.T01:CDS
GGATTTGAGGAATGCTATGA+TGG 0.566479 7:+70279223 MsG0780039903.01.T01:CDS
ATGCTATGATGGTGAGGATG+TGG 0.572731 7:+70279234 MsG0780039903.01.T01:CDS
TGTGGTTTACGTCGGTGAAG+AGG 0.589418 7:+70279033 MsG0780039903.01.T01:CDS
TAGCGAGGAGGTCGGAAGTG+AGG 0.591060 7:+70279099 MsG0780039903.01.T01:CDS
TGAGGAATGCTATGATGGTG+AGG 0.593394 7:+70279228 MsG0780039903.01.T01:CDS
TTTCAGGATGTGGTTTACGT+CGG 0.616502 7:+70279025 None:intergenic
TTCAGCAGTAGCGAGGAGGT+CGG 0.617278 7:+70279091 MsG0780039903.01.T01:CDS
GGTTTACGTCGGTGAAGAGG+AGG 0.624023 7:+70279036 MsG0780039903.01.T01:CDS
ATGGAGGTTGTGAAAAGGTG+GGG 0.628996 7:+70279155 MsG0780039903.01.T01:CDS
AAGTGAGGAAGGTGAAGACG+GGG 0.649652 7:+70279114 MsG0780039903.01.T01:CDS
AGAGGAGGTTAGGGCAACGA+AGG 0.654398 7:+70279051 MsG0780039903.01.T01:CDS
GAGAGTCTTCAGCAGTAGCG+AGG 0.658786 7:+70279084 MsG0780039903.01.T01:CDS
AGTCTTCAGCAGTAGCGAGG+AGG 0.690004 7:+70279087 MsG0780039903.01.T01:CDS
AGTGAGGAAGGTGAAGACGG+GGG 0.690327 7:+70279115 MsG0780039903.01.T01:CDS
GCTACTGCTGAAGACTCTCG+CGG 0.739378 7:-70279080 None:intergenic
CAAATCCCACTCCTTCACCG+AGG 0.758427 7:-70279207 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GGATTTGAGGAATGCTATGA+TGG + Chr7:70279223-70279242 MsG0780039903.01.T01:CDS 40.0%
AGATGGAGGTTGTGAAAAGG+TGG + Chr7:70279153-70279172 MsG0780039903.01.T01:CDS 45.0%
ATGGAGGTTGTGAAAAGGTG+GGG + Chr7:70279155-70279174 MsG0780039903.01.T01:CDS 45.0%
GATGGAGGTTGTGAAAAGGT+GGG + Chr7:70279154-70279173 MsG0780039903.01.T01:CDS 45.0%
TTGTGAAAAGGTGGGGAGAA+TGG + Chr7:70279162-70279181 MsG0780039903.01.T01:CDS 45.0%
! ATGCTATGATGGTGAGGATG+TGG + Chr7:70279234-70279253 MsG0780039903.01.T01:CDS 45.0%
! TGAGGAATGCTATGATGGTG+AGG + Chr7:70279228-70279247 MsG0780039903.01.T01:CDS 45.0%
!! AGGATGTGGTTGATGGTATC+TGG + Chr7:70279248-70279267 MsG0780039903.01.T01:CDS 45.0%
!! GGATGTGGTTGATGGTATCT+GGG + Chr7:70279249-70279268 MsG0780039903.01.T01:CDS 45.0%
AAGTGAGGAAGGTGAAGACG+GGG + Chr7:70279114-70279133 MsG0780039903.01.T01:CDS 50.0%
GAAGTGAGGAAGGTGAAGAC+GGG + Chr7:70279113-70279132 MsG0780039903.01.T01:CDS 50.0%
GATGGTGAGGATGTGGTTGA+TGG + Chr7:70279241-70279260 MsG0780039903.01.T01:CDS 50.0%
GGAAGTGAGGAAGGTGAAGA+CGG + Chr7:70279112-70279131 MsG0780039903.01.T01:CDS 50.0%
GGGAGATGGAGGTTGTGAAA+AGG + Chr7:70279150-70279169 MsG0780039903.01.T01:CDS 50.0%
GGTTAGGGCAACGAAGGATA+AGG + Chr7:70279057-70279076 MsG0780039903.01.T01:CDS 50.0%
TGAAAAGGTGGGGAGAATGG+TGG + Chr7:70279165-70279184 MsG0780039903.01.T01:CDS 50.0%
TGTGGTTTACGTCGGTGAAG+AGG + Chr7:70279033-70279052 MsG0780039903.01.T01:CDS 50.0%
ACGTCGGTGAAGAGGAGGTT+AGG + Chr7:70279041-70279060 MsG0780039903.01.T01:CDS 55.0%
AGAGGAGGTTAGGGCAACGA+AGG + Chr7:70279051-70279070 MsG0780039903.01.T01:CDS 55.0%
AGTCTTCAGCAGTAGCGAGG+AGG + Chr7:70279087-70279106 MsG0780039903.01.T01:CDS 55.0%
AGTGAGGAAGGTGAAGACGG+GGG + Chr7:70279115-70279134 MsG0780039903.01.T01:CDS 55.0%
CAAATCCCACTCCTTCACCG+AGG - Chr7:70279210-70279229 None:intergenic 55.0%
CGGTGAAGGAGTGGGATTTG+AGG + Chr7:70279210-70279229 MsG0780039903.01.T01:CDS 55.0%
CGTCGGTGAAGAGGAGGTTA+GGG + Chr7:70279042-70279061 MsG0780039903.01.T01:CDS 55.0%
GAGAGTCTTCAGCAGTAGCG+AGG + Chr7:70279084-70279103 MsG0780039903.01.T01:CDS 55.0%
GCTACTGCTGAAGACTCTCG+CGG - Chr7:70279083-70279102 None:intergenic 55.0%
GGTTTACGTCGGTGAAGAGG+AGG + Chr7:70279036-70279055 MsG0780039903.01.T01:CDS 55.0%
TTCAGCAGTAGCGAGGAGGT+CGG + Chr7:70279091-70279110 MsG0780039903.01.T01:CDS 55.0%
GAAGGTGAAGACGGGGGAGA+GGG + Chr7:70279121-70279140 MsG0780039903.01.T01:CDS 60.0%
GAGGAGGTCGGAAGTGAGGA+AGG + Chr7:70279103-70279122 MsG0780039903.01.T01:CDS 60.0%
GTCTGCCTCGGTGAAGGAGT+GGG + Chr7:70279202-70279221 MsG0780039903.01.T01:CDS 60.0%
TAGCGAGGAGGTCGGAAGTG+AGG + Chr7:70279099-70279118 MsG0780039903.01.T01:CDS 60.0%
TGTCTGCCTCGGTGAAGGAG+TGG + Chr7:70279201-70279220 MsG0780039903.01.T01:CDS 60.0%
!! GAGAATGGTGGACTAGCCGG+GGG + Chr7:70279177-70279196 MsG0780039903.01.T01:CDS 60.0%
!! GGAGAATGGTGGACTAGCCG+GGG + Chr7:70279176-70279195 MsG0780039903.01.T01:CDS 60.0%
!! GGGAGAATGGTGGACTAGCC+GGG + Chr7:70279175-70279194 MsG0780039903.01.T01:CDS 60.0%
!! GGGGAGAATGGTGGACTAGC+CGG + Chr7:70279174-70279193 MsG0780039903.01.T01:CDS 60.0%
AAGACGGGGGAGAGGGAGGT+AGG + Chr7:70279128-70279147 MsG0780039903.01.T01:CDS 65.0%
AGACGGGGGAGAGGGAGGTA+GGG + Chr7:70279129-70279148 MsG0780039903.01.T01:CDS 65.0%
GAGGGAGGTAGGGGAGATGG+AGG + Chr7:70279139-70279158 MsG0780039903.01.T01:CDS 65.0%
GGAAGGTGAAGACGGGGGAG+AGG + Chr7:70279120-70279139 MsG0780039903.01.T01:CDS 65.0%
GGAGAGGGAGGTAGGGGAGA+TGG + Chr7:70279136-70279155 MsG0780039903.01.T01:CDS 65.0%
GGGGATGTCTGCCTCGGTGA+AGG + Chr7:70279196-70279215 MsG0780039903.01.T01:CDS 65.0%
TAGCCGGGGGATGTCTGCCT+CGG + Chr7:70279190-70279209 MsG0780039903.01.T01:CDS 65.0%
TCACCGAGGCAGACATCCCC+CGG - Chr7:70279196-70279215 None:intergenic 65.0%
GACGGGGGAGAGGGAGGTAG+GGG + Chr7:70279130-70279149 MsG0780039903.01.T01:CDS 70.0%
GGTGAAGACGGGGGAGAGGG+AGG + Chr7:70279124-70279143 MsG0780039903.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 70279032 70279274 70279032 ID=MsG0780039903.01;Name=MsG0780039903.01
Chr7 mRNA 70279032 70279274 70279032 ID=MsG0780039903.01.T01;Parent=MsG0780039903.01;Name=MsG0780039903.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|80
Chr7 exon 70279032 70279274 70279032 ID=MsG0780039903.01.T01:exon:13078;Parent=MsG0780039903.01.T01
Chr7 CDS 70279032 70279274 70279032 ID=MsG0780039903.01.T01:cds;Parent=MsG0780039903.01.T01
Gene Sequence

>MsG0780039903.01.T01

ATGTGGTTTACGTCGGTGAAGAGGAGGTTAGGGCAACGAAGGATAAGGCCGCGAGAGTCTTCAGCAGTAGCGAGGAGGTCGGAAGTGAGGAAGGTGAAGACGGGGGAGAGGGAGGTAGGGGAGATGGAGGTTGTGAAAAGGTGGGGAGAATGGTGGACTAGCCGGGGGATGTCTGCCTCGGTGAAGGAGTGGGATTTGAGGAATGCTATGATGGTGAGGATGTGGTTGATGGTATCTGGGTGA

Protein sequence

>MsG0780039903.01.T01

MWFTSVKRRLGQRRIRPRESSAVARRSEVRKVKTGEREVGEMEVVKRWGEWWTSRGMSASVKEWDLRNAMMVRMWLMVSG*