AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039902.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039902.01.T01 MTR_7g081270 97.087 103 3 0 1 103 182 284 4.28e-71 213
MsG0780039902.01.T01 MTR_2g049780 50.526 95 46 1 4 98 211 304 7.88e-26 97.8
MsG0780039902.01.T01 MTR_5g041630 47.619 84 44 0 12 95 206 289 5.06e-20 82.4
MsG0780039902.01.T01 MTR_2g067230 43.750 80 45 0 12 91 213 292 7.08e-18 77.0
MsG0780039902.01.T01 MTR_6g003960 35.165 91 59 0 12 102 501 591 5.37e-14 66.6
MsG0780039902.01.T01 MTR_2g034600 41.304 92 51 2 7 98 455 543 1.51e-13 65.1
MsG0780039902.01.T01 MTR_2g034600 41.304 92 51 2 7 98 459 547 1.54e-13 65.1
MsG0780039902.01.T01 MTR_2g034600 41.304 92 51 2 7 98 394 482 2.75e-13 64.3
MsG0780039902.01.T01 MTR_4g070060 36.782 87 54 1 12 98 500 585 4.16e-12 61.2
MsG0780039902.01.T01 MTR_4g084630 36.264 91 58 0 12 102 232 322 6.40e-12 60.5
MsG0780039902.01.T01 MTR_5g068860 33.673 98 63 2 1 98 387 482 4.37e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039902.01.T01 AT3G18870 65.049 103 35 1 1 103 172 273 1.28e-43 143
MsG0780039902.01.T01 AT2G03050 49.474 95 48 0 4 98 174 268 8.68e-27 100
MsG0780039902.01.T01 AT2G03050 49.474 95 48 0 4 98 175 269 8.84e-27 100
MsG0780039902.01.T01 AT2G34620 48.421 95 48 1 4 98 197 290 9.49e-25 95.1
MsG0780039902.01.T01 AT2G36000 41.053 95 55 1 4 98 214 307 1.96e-17 75.9
MsG0780039902.01.T01 AT2G36000 41.053 95 55 1 4 98 214 307 4.03e-17 74.7
MsG0780039902.01.T01 AT1G78930 39.394 99 59 1 4 102 481 578 3.03e-15 70.1
MsG0780039902.01.T01 AT2G21710 39.080 87 52 1 12 98 507 592 5.25e-13 63.9
MsG0780039902.01.T01 AT5G55580 36.893 103 59 4 4 102 262 362 1.79e-12 62.0
MsG0780039902.01.T01 AT4G14605 40.000 85 48 2 7 91 379 460 5.33e-12 60.8
MsG0780039902.01.T01 AT4G14605 40.000 85 48 2 7 91 379 460 5.33e-12 60.8
MsG0780039902.01.T01 AT5G55580 36.893 103 59 4 4 102 368 468 1.43e-11 59.7

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAGGTTGCCGGCGATAT+TGG 0.211818 7:-70278838 MsG0780039902.01.T01:CDS
GCAAGGTTGCCGGCGATATT+GGG 0.271051 7:-70278837 MsG0780039902.01.T01:CDS
GTGGCCGAAGTTTGTGTATT+TGG 0.292279 7:-70278793 MsG0780039902.01.T01:CDS
GAATGTTTGTGCAAGGTTGC+CGG 0.395975 7:-70278847 MsG0780039902.01.T01:CDS
ATTTGAAAGAAAGAGGTGTT+AGG 0.409873 7:-70278677 MsG0780039902.01.T01:CDS
GAAAGAGATGGAGAGGGAGG+TGG 0.414641 7:-70278769 MsG0780039902.01.T01:CDS
AGGTTTCTTGAGGAAGTGGT+TGG 0.427900 7:-70278891 MsG0780039902.01.T01:CDS
TCTTTCAAATGCAAATGCCT+TGG 0.434972 7:+70278690 None:intergenic
CATTTGCATTTGAAAGAAAG+AGG 0.462822 7:-70278684 MsG0780039902.01.T01:CDS
GGTTTCTTGAGGAAGTGGTT+GGG 0.466379 7:-70278890 MsG0780039902.01.T01:CDS
GTTGAGGTTTCTTGAGGAAG+TGG 0.478350 7:-70278895 MsG0780039902.01.T01:CDS
AGAAGATGCGCATGAGGTTG+AGG 0.495323 7:-70278911 None:intergenic
TTGGGTTTAGTTTGGAGAAG+AGG 0.509649 7:-70278719 MsG0780039902.01.T01:CDS
TTCACCAAATACACAAACTT+CGG 0.521850 7:+70278789 None:intergenic
ATTTGGTGAAAGAGATGGAG+AGG 0.546039 7:-70278776 MsG0780039902.01.T01:CDS
CACATCAACATTCTATTCAA+AGG 0.546735 7:+70278651 None:intergenic
GGTGAAAGAGATGGAGAGGG+AGG 0.550774 7:-70278772 MsG0780039902.01.T01:CDS
CATGAGGTTGAGGTTTCTTG+AGG 0.557913 7:-70278901 MsG0780039902.01.T01:CDS
CAAGGTTGCCGGCGATATTG+GGG 0.558881 7:-70278836 MsG0780039902.01.T01:CDS
TGTGTATTTGGTGAAAGAGA+TGG 0.569565 7:-70278781 MsG0780039902.01.T01:CDS
TGGAGAAGAGGATTGTTCCA+AGG 0.570343 7:-70278707 MsG0780039902.01.T01:CDS
CTTTGAATAGAATGTTGATG+TGG 0.576656 7:-70278650 MsG0780039902.01.T01:CDS
TTTGAATAGAATGTTGATGT+GGG 0.578021 7:-70278649 MsG0780039902.01.T01:CDS
TTTGGTGAAAGAGATGGAGA+GGG 0.581785 7:-70278775 MsG0780039902.01.T01:CDS
AAGCGAGGAATGTTTGTGCA+AGG 0.596914 7:-70278854 MsG0780039902.01.T01:CDS
AGTGGTTGGGTTTACGTACG+AGG 0.606381 7:-70278877 MsG0780039902.01.T01:CDS
ATGATAAGTTCTATGCAAAG+TGG 0.608848 7:-70278623 MsG0780039902.01.T01:CDS
ACATCGTACCCCAATATCGC+CGG 0.610968 7:+70278828 None:intergenic
GGTTTACGTACGAGGAAGCG+AGG 0.621107 7:-70278869 MsG0780039902.01.T01:CDS
GGCGATATTGGGGTACGATG+TGG 0.628281 7:-70278826 MsG0780039902.01.T01:CDS
GTGTGGAGAAGATGCGCATG+AGG 0.685391 7:-70278917 None:intergenic
ACGATGTGGAGAATAACTTG+TGG 0.706381 7:-70278812 MsG0780039902.01.T01:CDS
TTGAATAGAATGTTGATGTG+GGG 0.710612 7:-70278648 MsG0780039902.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTGAATAGAATGTTGATGT+GGG - Chr7:70278874-70278893 MsG0780039902.01.T01:CDS 25.0%
AAACTAAACCCAAAAAACTG+CGG + Chr7:70278797-70278816 None:intergenic 30.0%
ATGATAAGTTCTATGCAAAG+TGG - Chr7:70278900-70278919 MsG0780039902.01.T01:CDS 30.0%
CACATCAACATTCTATTCAA+AGG + Chr7:70278875-70278894 None:intergenic 30.0%
CATTTGCATTTGAAAGAAAG+AGG - Chr7:70278839-70278858 MsG0780039902.01.T01:CDS 30.0%
CTTTGAATAGAATGTTGATG+TGG - Chr7:70278873-70278892 MsG0780039902.01.T01:CDS 30.0%
TTCACCAAATACACAAACTT+CGG + Chr7:70278737-70278756 None:intergenic 30.0%
TTGAATAGAATGTTGATGTG+GGG - Chr7:70278875-70278894 MsG0780039902.01.T01:CDS 30.0%
!! ATTTGAAAGAAAGAGGTGTT+AGG - Chr7:70278846-70278865 MsG0780039902.01.T01:CDS 30.0%
TCTTTCAAATGCAAATGCCT+TGG + Chr7:70278836-70278855 None:intergenic 35.0%
! TGTGTATTTGGTGAAAGAGA+TGG - Chr7:70278742-70278761 MsG0780039902.01.T01:CDS 35.0%
!!! AAGAAGTTTCCGCAGTTTTT+TGG - Chr7:70278785-70278804 MsG0780039902.01.T01:CDS 35.0%
!!! AGAAGTTTCCGCAGTTTTTT+GGG - Chr7:70278786-70278805 MsG0780039902.01.T01:CDS 35.0%
!!! GCAGTTTTTTGGGTTTAGTT+TGG - Chr7:70278796-70278815 MsG0780039902.01.T01:CDS 35.0%
ACGATGTGGAGAATAACTTG+TGG - Chr7:70278711-70278730 MsG0780039902.01.T01:CDS 40.0%
TTTGGTGAAAGAGATGGAGA+GGG - Chr7:70278748-70278767 MsG0780039902.01.T01:CDS 40.0%
! ATTTGGTGAAAGAGATGGAG+AGG - Chr7:70278747-70278766 MsG0780039902.01.T01:CDS 40.0%
!! TTGGGTTTAGTTTGGAGAAG+AGG - Chr7:70278804-70278823 MsG0780039902.01.T01:CDS 40.0%
AAGCGAGGAATGTTTGTGCA+AGG - Chr7:70278669-70278688 MsG0780039902.01.T01:CDS 45.0%
AGGTTTCTTGAGGAAGTGGT+TGG - Chr7:70278632-70278651 MsG0780039902.01.T01:CDS 45.0%
CATGAGGTTGAGGTTTCTTG+AGG - Chr7:70278622-70278641 MsG0780039902.01.T01:CDS 45.0%
GAATGTTTGTGCAAGGTTGC+CGG - Chr7:70278676-70278695 MsG0780039902.01.T01:CDS 45.0%
GGTTTCTTGAGGAAGTGGTT+GGG - Chr7:70278633-70278652 MsG0780039902.01.T01:CDS 45.0%
GTGGCCGAAGTTTGTGTATT+TGG - Chr7:70278730-70278749 MsG0780039902.01.T01:CDS 45.0%
GTTGAGGTTTCTTGAGGAAG+TGG - Chr7:70278628-70278647 MsG0780039902.01.T01:CDS 45.0%
TGGAGAAGAGGATTGTTCCA+AGG - Chr7:70278816-70278835 MsG0780039902.01.T01:CDS 45.0%
ACATCGTACCCCAATATCGC+CGG + Chr7:70278698-70278717 None:intergenic 50.0%
AGTGGTTGGGTTTACGTACG+AGG - Chr7:70278646-70278665 MsG0780039902.01.T01:CDS 50.0%
CAAGGTTGCCGGCGATATTG+GGG - Chr7:70278687-70278706 MsG0780039902.01.T01:CDS 55.0%
GAAAGAGATGGAGAGGGAGG+TGG - Chr7:70278754-70278773 MsG0780039902.01.T01:CDS 55.0%
GCAAGGTTGCCGGCGATATT+GGG - Chr7:70278686-70278705 MsG0780039902.01.T01:CDS 55.0%
GGCGATATTGGGGTACGATG+TGG - Chr7:70278697-70278716 MsG0780039902.01.T01:CDS 55.0%
GGTGAAAGAGATGGAGAGGG+AGG - Chr7:70278751-70278770 MsG0780039902.01.T01:CDS 55.0%
GGTTTACGTACGAGGAAGCG+AGG - Chr7:70278654-70278673 MsG0780039902.01.T01:CDS 55.0%
TGCAAGGTTGCCGGCGATAT+TGG - Chr7:70278685-70278704 MsG0780039902.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 70278617 70278928 70278617 ID=MsG0780039902.01;Name=MsG0780039902.01
Chr7 mRNA 70278617 70278928 70278617 ID=MsG0780039902.01.T01;Parent=MsG0780039902.01;Name=MsG0780039902.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|103
Chr7 exon 70278617 70278928 70278617 ID=MsG0780039902.01.T01:exon:13131;Parent=MsG0780039902.01.T01
Chr7 CDS 70278617 70278928 70278617 ID=MsG0780039902.01.T01:cds;Parent=MsG0780039902.01.T01
Gene Sequence

>MsG0780039902.01.T01

ATGCGCATGAGGTTGAGGTTTCTTGAGGAAGTGGTTGGGTTTACGTACGAGGAAGCGAGGAATGTTTGTGCAAGGTTGCCGGCGATATTGGGGTACGATGTGGAGAATAACTTGTGGCCGAAGTTTGTGTATTTGGTGAAAGAGATGGAGAGGGAGGTGGAAGAGTTGAAGAAGTTTCCGCAGTTTTTTGGGTTTAGTTTGGAGAAGAGGATTGTTCCAAGGCATTTGCATTTGAAAGAAAGAGGTGTTAGGATTCCTTTGAATAGAATGTTGATGTGGGGTGATGATAAGTTCTATGCAAAGTGGAAGTGA

Protein sequence

>MsG0780039902.01.T01

MRMRLRFLEEVVGFTYEEARNVCARLPAILGYDVENNLWPKFVYLVKEMEREVEELKKFPQFFGFSLEKRIVPRHLHLKERGVRIPLNRMLMWGDDKFYAKWK*