AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025315.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025315.01.T01 MTR_5g026290 93.333 135 8 1 1 134 1 135 2.80e-91 260
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTTGTACTTTGTTAATT+TGG 0.112303 5:+16869182 None:intergenic
CTTTGTTAATTTGGAATAAC+TGG 0.189219 5:+16869191 None:intergenic
TCTTTGAAGTTGCTTTCTTC+AGG 0.231770 5:+16869437 None:intergenic
ATGCATTGTGCAGCTTCTTA+TGG 0.341347 5:-16869536 MsG0580025315.01.T01:CDS
AAGGGATCATCACATGAAAT+AGG 0.348210 5:-16869404 MsG0580025315.01.T01:CDS
CTGCACAATGCATTAGTGTT+TGG 0.388403 5:+16869546 None:intergenic
CTTTGAAGTTGCTTTCTTCA+GGG 0.422990 5:+16869438 None:intergenic
GCACAAAAGCTTCACAGGTT+GGG 0.439585 5:-16869353 MsG0580025315.01.T01:CDS
CTCAAGCACAAAAGCTTCAC+AGG 0.495602 5:-16869358 MsG0580025315.01.T01:CDS
GCAGCTTCTTATGGTGAAAG+AGG 0.521502 5:-16869527 MsG0580025315.01.T01:CDS
AGCACAAAAGCTTCACAGGT+TGG 0.529568 5:-16869354 MsG0580025315.01.T01:CDS
TGCTAGTGAATCAAGGATGA+TGG 0.543307 5:-16869252 MsG0580025315.01.T01:CDS
CCATCTCGTCGCAAAAGCTA+TGG 0.544204 5:-16869491 MsG0580025315.01.T01:CDS
GAGATGTTGCTAGTGAATCA+AGG 0.609919 5:-16869259 MsG0580025315.01.T01:CDS
GCTAGTGAATCAAGGATGAT+GGG 0.613297 5:-16869251 MsG0580025315.01.T01:CDS
ATTCACTAGCAACATCTCCA+AGG 0.613391 5:+16869264 None:intergenic
GATGGGAATTGATGCACCAA+TGG 0.622941 5:-16869234 MsG0580025315.01.T01:CDS
CGATGCTATTCTCTACTCGA+CGG 0.702175 5:+16869465 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTTACATAGTAATTA+AGG - Chr5:16869465-16869484 MsG0580025315.01.T01:CDS 15.0%
!! TTCAAAGAACAAAAAAACAA+GGG - Chr5:16869352-16869371 MsG0580025315.01.T01:CDS 20.0%
! ATAACTGGAACAGAAAAATA+TGG + Chr5:16869571-16869590 None:intergenic 25.0%
! CTTCAAAGAACAAAAAAACA+AGG - Chr5:16869351-16869370 MsG0580025315.01.T01:CDS 25.0%
!!! AAGGTTAAAGCATTTTACAA+TGG - Chr5:16869484-16869503 MsG0580025315.01.T01:CDS 25.0%
AAGGGATCATCACATGAAAT+AGG - Chr5:16869370-16869389 MsG0580025315.01.T01:CDS 35.0%
! GAAAAATATGGTTCTGCCAT+TGG + Chr5:16869559-16869578 None:intergenic 35.0%
! GCATTTTACAATGGTTTCCT+TGG - Chr5:16869493-16869512 MsG0580025315.01.T01:CDS 35.0%
!! CTTTGAAGTTGCTTTCTTCA+GGG + Chr5:16869339-16869358 None:intergenic 35.0%
!! TCTTTGAAGTTGCTTTCTTC+AGG + Chr5:16869340-16869359 None:intergenic 35.0%
ATGCATTGTGCAGCTTCTTA+TGG - Chr5:16869238-16869257 MsG0580025315.01.T01:CDS 40.0%
ATTCACTAGCAACATCTCCA+AGG + Chr5:16869513-16869532 None:intergenic 40.0%
CTGCACAATGCATTAGTGTT+TGG + Chr5:16869231-16869250 None:intergenic 40.0%
GAGATGTTGCTAGTGAATCA+AGG - Chr5:16869515-16869534 MsG0580025315.01.T01:CDS 40.0%
GCTAGTGAATCAAGGATGAT+GGG - Chr5:16869523-16869542 MsG0580025315.01.T01:CDS 40.0%
TGCTAGTGAATCAAGGATGA+TGG - Chr5:16869522-16869541 MsG0580025315.01.T01:CDS 40.0%
AGCACAAAAGCTTCACAGGT+TGG - Chr5:16869420-16869439 MsG0580025315.01.T01:CDS 45.0%
CGATGCTATTCTCTACTCGA+CGG + Chr5:16869312-16869331 None:intergenic 45.0%
CTCAAGCACAAAAGCTTCAC+AGG - Chr5:16869416-16869435 MsG0580025315.01.T01:CDS 45.0%
GATGGGAATTGATGCACCAA+TGG - Chr5:16869540-16869559 MsG0580025315.01.T01:CDS 45.0%
GCACAAAAGCTTCACAGGTT+GGG - Chr5:16869421-16869440 MsG0580025315.01.T01:CDS 45.0%
GCAGCTTCTTATGGTGAAAG+AGG - Chr5:16869247-16869266 MsG0580025315.01.T01:CDS 45.0%
CCATCTCGTCGCAAAAGCTA+TGG - Chr5:16869283-16869302 MsG0580025315.01.T01:CDS 50.0%
! CCATAGCTTTTGCGACGAGA+TGG + Chr5:16869286-16869305 None:intergenic 50.0%
! CTTTTGCGACGAGATGGTGT+TGG + Chr5:16869280-16869299 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 16869196 16869600 16869196 ID=MsG0580025315.01;Name=MsG0580025315.01
Chr5 mRNA 16869196 16869600 16869196 ID=MsG0580025315.01.T01;Parent=MsG0580025315.01;Name=MsG0580025315.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|134
Chr5 exon 16869196 16869600 16869196 ID=MsG0580025315.01.T01:exon:22794;Parent=MsG0580025315.01.T01
Chr5 CDS 16869196 16869600 16869196 ID=MsG0580025315.01.T01:cds;Parent=MsG0580025315.01.T01
Gene Sequence

>MsG0580025315.01.T01

ATGGCTTCACAACTTGTTAGTGATGTCAAATGCCAAACACTAATGCATTGTGCAGCTTCTTATGGTGAAAGAGGAAGCAAACCAACACCATCTCGTCGCAAAAGCTATGGCTACCGTCGAGTAGAGAATAGCATCGTCTACCCTGAAGAAAGCAACTTCAAAGAACAAAAAAACAAGGGATCATCACATGAAATAGGTGTAAAACGTAACACAAATCTAGCTCAAGCACAAAAGCTTCACAGGTTGGGATTCTCTTACATTGCAACATCTTTTCTTTACATAGTAATTAAGGTTAAAGCATTTTACAATGGTTTCCTTGGAGATGTTGCTAGTGAATCAAGGATGATGGGAATTGATGCACCAATGGCAGAACCATATTTTTCTGTTCCAGTTATTCCAAATTAA

Protein sequence

>MsG0580025315.01.T01

MASQLVSDVKCQTLMHCAASYGERGSKPTPSRRKSYGYRRVENSIVYPEESNFKEQKNKGSSHEIGVKRNTNLAQAQKLHRLGFSYIATSFLYIVIKVKAFYNGFLGDVASESRMMGIDAPMAEPYFSVPVIPN*