AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010704.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010704.01.T01 MTR_2g090200 98.810 84 1 0 1 84 87 170 1.28e-57 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010704.01.T01 AT1G03600 80.952 84 16 0 1 84 91 174 6.06e-47 147

Find 51 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGGGTGGGAGTGATTT+GGG 0.211697 2:-73667940 None:intergenic
TCTGCACTCAATGCTGTTTC+AGG 0.229074 2:+73668296 MsG0280010704.01.T01:CDS
TTTGATGGGTGGGAGTGATT+TGG 0.246351 2:-73667941 None:intergenic
GTGTGTGGTGGTGGAGGTGG+TGG 0.265783 2:-73668001 None:intergenic
TCAGGTCATTATATTAGTTT+TGG 0.273668 2:+73668314 MsG0280010704.01.T01:CDS
GGAGGTGGTGGTTGGTGTTA+AGG 0.288700 2:-73667989 None:intergenic
GGCTGAGATGGTGGTGAGTT+TGG 0.303203 2:-73667965 None:intergenic
ATCATTCTTGTCCATTGTTA+TGG 0.319763 2:-73668162 None:intergenic
GGGGTGTGTGGTGGTGGAGG+TGG 0.320233 2:-73668004 None:intergenic
GAGTGATTTGGGTGTGGAAG+TGG 0.337912 2:-73667929 None:intergenic
TGAAAGAGAGGGAGAGAGAG+TGG 0.346543 2:-73667873 None:intergenic
GCAACTGCAGTAGCTACATT+TGG 0.393423 2:-73668185 None:intergenic
TGAGAGGAACTCACGGCGGC+GGG 0.402734 2:-73668025 None:intergenic
AGTGATTTGGGTGTGGAAGT+GGG 0.410586 2:-73667928 None:intergenic
TCATCTGATGCTGCTAATGC+AGG 0.411219 2:-73668092 None:intergenic
GTGGTGGTGGAGGTGGTGGT+TGG 0.411829 2:-73667997 None:intergenic
TGGTGTTAAGGTGGCTGAGA+TGG 0.425709 2:-73667977 None:intergenic
AGAGGAACTCACGGCGGCGG+GGG 0.446188 2:-73668023 None:intergenic
ACTCTTCTCTTAGCAGGAAT+AGG 0.452938 2:-73668347 None:intergenic
TTGAGAGGAACTCACGGCGG+CGG 0.457527 2:-73668026 None:intergenic
GGTGGTGAGGATGGTGGCTG+TGG 0.457592 2:-73668049 None:intergenic
ATCCTCACTCTTCTCTTAGC+AGG 0.465100 2:-73668353 None:intergenic
GGTGGTGGTTGGTGTTAAGG+TGG 0.491347 2:-73667986 None:intergenic
TCACTTAAAAGAGGAAGATA+AGG 0.497770 2:+73668404 MsG0280010704.01.T01:CDS
GGGTGGGAGTGATTTGGGTG+TGG 0.504139 2:-73667935 None:intergenic
TAAGAGACACTTCAAACTCA+TGG 0.508045 2:+73668213 MsG0280010704.01.T01:CDS
TAGAAATGTGGTGGTGAGGA+TGG 0.520819 2:-73668058 None:intergenic
GCTGAGAAATCACTTAAAAG+AGG 0.527965 2:+73668395 MsG0280010704.01.T01:CDS
GAAAGAGAGGGAGAGAGAGT+GGG 0.533921 2:-73667872 None:intergenic
TTAGCAGGAATAGGAGCAGT+TGG 0.542214 2:-73668338 None:intergenic
TGTTAAGGTGGCTGAGATGG+TGG 0.551855 2:-73667974 None:intergenic
AACAGAGGAGGTTATAAGCA+AGG 0.555080 2:+73668130 MsG0280010704.01.T01:five_prime_UTR
TATTAGTGTTGGTGAAGCCA+TGG 0.567643 2:-73667902 None:intergenic
CCATGGTTGTGTTGAAAGAG+AGG 0.578065 2:-73667885 None:intergenic
AAGAGACACTTCAAACTCAT+GGG 0.579817 2:+73668214 MsG0280010704.01.T01:CDS
GAGAGGAACTCACGGCGGCG+GGG 0.588141 2:-73668024 None:intergenic
GGTAAGAACAACCATAACAA+TGG 0.607047 2:+73668151 MsG0280010704.01.T01:exon
CCTCTCTTTCAACACAACCA+TGG 0.613506 2:+73667885 MsG0280010704.01.T01:five_prime_UTR
GGATGGTGGCTGTGGTTGAG+AGG 0.617289 2:-73668041 None:intergenic
CTGTGGTTGAGAGGAACTCA+CGG 0.621671 2:-73668032 None:intergenic
GAAGTGGGAGTTATTAGTGT+TGG 0.625695 2:-73667913 None:intergenic
GAGATAGAAATGTGGTGGTG+AGG 0.626819 2:-73668062 None:intergenic
AAATGTGGTGGTGAGGATGG+TGG 0.634984 2:-73668055 None:intergenic
CATCTGATGCTGCTAATGCA+GGG 0.635954 2:-73668091 None:intergenic
CATGGTTGTGTTGAAAGAGA+GGG 0.643426 2:-73667884 None:intergenic
AGAGTACGTGAAAGAAACAG+AGG 0.659125 2:+73668115 MsG0280010704.01.T01:five_prime_UTR
GTACGTGAAAGAAACAGAGG+AGG 0.680949 2:+73668118 MsG0280010704.01.T01:five_prime_UTR
GACTTGAGATAGAAATGTGG+TGG 0.682515 2:-73668067 None:intergenic
GGTGACTTGAGATAGAAATG+TGG 0.772012 2:-73668070 None:intergenic
TTCCTGCTAAGAGAAGAGTG+AGG 0.789481 2:+73668351 MsG0280010704.01.T01:CDS
TGGTTGAGAGGAACTCACGG+CGG 0.826855 2:-73668029 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAGGTCATTATATTAGTTT+TGG + Chr2:73668314-73668333 MsG0280010704.01.T01:CDS 25.0%
ATCATTCTTGTCCATTGTTA+TGG - Chr2:73668165-73668184 None:intergenic 30.0%
AAGAGACACTTCAAACTCAT+GGG + Chr2:73668214-73668233 MsG0280010704.01.T01:CDS 35.0%
GCTGAGAAATCACTTAAAAG+AGG + Chr2:73668395-73668414 MsG0280010704.01.T01:CDS 35.0%
GGTAAGAACAACCATAACAA+TGG + Chr2:73668151-73668170 MsG0280010704.01.T01:exon 35.0%
TAAGAGACACTTCAAACTCA+TGG + Chr2:73668213-73668232 MsG0280010704.01.T01:CDS 35.0%
AACAGAGGAGGTTATAAGCA+AGG + Chr2:73668130-73668149 MsG0280010704.01.T01:five_prime_UTR 40.0%
ACTCTTCTCTTAGCAGGAAT+AGG - Chr2:73668350-73668369 None:intergenic 40.0%
AGAGTACGTGAAAGAAACAG+AGG + Chr2:73668115-73668134 MsG0280010704.01.T01:five_prime_UTR 40.0%
CATGGTTGTGTTGAAAGAGA+GGG - Chr2:73667887-73667906 None:intergenic 40.0%
GAAGTGGGAGTTATTAGTGT+TGG - Chr2:73667916-73667935 None:intergenic 40.0%
GACTTGAGATAGAAATGTGG+TGG - Chr2:73668070-73668089 None:intergenic 40.0%
GGTGACTTGAGATAGAAATG+TGG - Chr2:73668073-73668092 None:intergenic 40.0%
! GAGAAGAGTGAGGATTTTAG+AGG + Chr2:73668361-73668380 MsG0280010704.01.T01:CDS 40.0%
!! TATTAGTGTTGGTGAAGCCA+TGG - Chr2:73667905-73667924 None:intergenic 40.0%
!!! GTGGTGAGTTTGGTTTTGAT+GGG - Chr2:73667958-73667977 None:intergenic 40.0%
!!! TGAGTTTGGTTTTGATGGGT+GGG - Chr2:73667954-73667973 None:intergenic 40.0%
ATCCTCACTCTTCTCTTAGC+AGG - Chr2:73668356-73668375 None:intergenic 45.0%
CATCTGATGCTGCTAATGCA+GGG - Chr2:73668094-73668113 None:intergenic 45.0%
CCATGGTTGTGTTGAAAGAG+AGG - Chr2:73667888-73667907 None:intergenic 45.0%
CCTCTCTTTCAACACAACCA+TGG + Chr2:73667885-73667904 MsG0280010704.01.T01:five_prime_UTR 45.0%
GCAACTGCAGTAGCTACATT+TGG - Chr2:73668188-73668207 None:intergenic 45.0%
GTACGTGAAAGAAACAGAGG+AGG + Chr2:73668118-73668137 MsG0280010704.01.T01:five_prime_UTR 45.0%
TCATCTGATGCTGCTAATGC+AGG - Chr2:73668095-73668114 None:intergenic 45.0%
TTAGCAGGAATAGGAGCAGT+TGG - Chr2:73668341-73668360 None:intergenic 45.0%
TTCCTGCTAAGAGAAGAGTG+AGG + Chr2:73668351-73668370 MsG0280010704.01.T01:CDS 45.0%
! AGTGATTTGGGTGTGGAAGT+GGG - Chr2:73667931-73667950 None:intergenic 45.0%
! GAGATAGAAATGTGGTGGTG+AGG - Chr2:73668065-73668084 None:intergenic 45.0%
! TAGAAATGTGGTGGTGAGGA+TGG - Chr2:73668061-73668080 None:intergenic 45.0%
! TTGATGGGTGGGAGTGATTT+GGG - Chr2:73667943-73667962 None:intergenic 45.0%
! TTTGATGGGTGGGAGTGATT+TGG - Chr2:73667944-73667963 None:intergenic 45.0%
!! TCTGCACTCAATGCTGTTTC+AGG + Chr2:73668296-73668315 MsG0280010704.01.T01:CDS 45.0%
!!! GGTGGTGAGTTTGGTTTTGA+TGG - Chr2:73667959-73667978 None:intergenic 45.0%
!!! GTGAGTTTGGTTTTGATGGG+TGG - Chr2:73667955-73667974 None:intergenic 45.0%
CTGTGGTTGAGAGGAACTCA+CGG - Chr2:73668035-73668054 None:intergenic 50.0%
GAAAGAGAGGGAGAGAGAGT+GGG - Chr2:73667875-73667894 None:intergenic 50.0%
TGAAAGAGAGGGAGAGAGAG+TGG - Chr2:73667876-73667895 None:intergenic 50.0%
TGTTAAGGTGGCTGAGATGG+TGG - Chr2:73667977-73667996 None:intergenic 50.0%
! AAATGTGGTGGTGAGGATGG+TGG - Chr2:73668058-73668077 None:intergenic 50.0%
! GAGTGATTTGGGTGTGGAAG+TGG - Chr2:73667932-73667951 None:intergenic 50.0%
! TGGTGTTAAGGTGGCTGAGA+TGG - Chr2:73667980-73667999 None:intergenic 50.0%
TGGTTGAGAGGAACTCACGG+CGG - Chr2:73668032-73668051 None:intergenic 55.0%
! GGCTGAGATGGTGGTGAGTT+TGG - Chr2:73667968-73667987 None:intergenic 55.0%
!! GGAGGTGGTGGTTGGTGTTA+AGG - Chr2:73667992-73668011 None:intergenic 55.0%
!! GGTGGTGGTTGGTGTTAAGG+TGG - Chr2:73667989-73668008 None:intergenic 55.0%
TTGAGAGGAACTCACGGCGG+CGG - Chr2:73668029-73668048 None:intergenic 60.0%
! GGATGGTGGCTGTGGTTGAG+AGG - Chr2:73668044-73668063 None:intergenic 60.0%
! GGGTGGGAGTGATTTGGGTG+TGG - Chr2:73667938-73667957 None:intergenic 60.0%
TGAGAGGAACTCACGGCGGC+GGG - Chr2:73668028-73668047 None:intergenic 65.0%
! GGTGGTGAGGATGGTGGCTG+TGG - Chr2:73668052-73668071 None:intergenic 65.0%
!! GTGGTGGTGGAGGTGGTGGT+TGG - Chr2:73668000-73668019 None:intergenic 65.0%
!! GTGTGTGGTGGTGGAGGTGG+TGG - Chr2:73668004-73668023 None:intergenic 65.0%
AGAGGAACTCACGGCGGCGG+GGG - Chr2:73668026-73668045 None:intergenic 70.0%
GAGAGGAACTCACGGCGGCG+GGG - Chr2:73668027-73668046 None:intergenic 70.0%
!! GGGGTGTGTGGTGGTGGAGG+TGG - Chr2:73668007-73668026 None:intergenic 70.0%
!! GCGGGGGTGTGTGGTGGTGG+AGG - Chr2:73668010-73668029 None:intergenic 75.0%
!! ACGGCGGCGGGGGTGTGTGG+TGG - Chr2:73668016-73668035 None:intergenic 80.0%
!! CTCACGGCGGCGGGGGTGTG+TGG - Chr2:73668019-73668038 None:intergenic 80.0%
!! GCGGCGGGGGTGTGTGGTGG+TGG - Chr2:73668013-73668032 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr2 gene 73667855 73668424 73667855 ID=MsG0280010704.01;Name=MsG0280010704.01
Chr2 mRNA 73667855 73668424 73667855 ID=MsG0280010704.01.T01;Parent=MsG0280010704.01;Name=MsG0280010704.01.T01;_AED=0.50;_eAED=0.50;_QI=315|-1|0|1|-1|1|1|0|84
Chr2 exon 73667855 73668424 73667855 ID=MsG0280010704.01.T01:exon:15505;Parent=MsG0280010704.01.T01
Chr2 five_prime_UTR 73667855 73668169 73667855 ID=MsG0280010704.01.T01:five_prime_utr;Parent=MsG0280010704.01.T01
Chr2 CDS 73668170 73668424 73668170 ID=MsG0280010704.01.T01:cds;Parent=MsG0280010704.01.T01
Gene Sequence

>MsG0280010704.01.T01

ATGGACAAGAATGATCCAAATGTAGCTACTGCAGTTGCTGAACTAAGAGACACTTCAAACTCATGGGTTGCTAAATATAGAAGAGAAAAAGCACTTCTTGCAAGAGCTTCTTTTAGAGATATGTACTCTGCACTCAATGCTGTTTCAGGTCATTATATTAGTTTTGGTCCAACTGCTCCTATTCCTGCTAAGAGAAGAGTGAGGATTTTAGAGGAAATTGAAGTTGCTGAGAAATCACTTAAAAGAGGAAGATAA

Protein sequence

>MsG0280010704.01.T01

MDKNDPNVATAVAELRDTSNSWVAKYRREKALLARASFRDMYSALNAVSGHYISFGPTAPIPAKRRVRILEEIEVAEKSLKRGR*