AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0035110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0035110 MtrunA17_Chr1g0197391 97.895 570 12 0 4 573 1 570 0.0 1144
Msa0035110 MtrunA17_Chr4g0028411 43.716 549 294 7 27 569 3 542 2.48e-152 448
Msa0035110 MtrunA17_Chr1g0157401 45.471 552 280 9 25 562 9 553 1.06e-151 447
Msa0035110 MtrunA17_Chr2g0327341 40.585 547 313 6 26 569 2 539 1.81e-144 428
Msa0035110 MtrunA17_Chr5g0409771 45.037 544 275 12 28 561 18 547 2.23e-142 423
Msa0035110 MtrunA17_Chr4g0039791 45.422 557 272 12 30 573 24 561 3.64e-142 422
Msa0035110 MtrunA17_Chr5g0409791 44.585 554 271 12 30 561 21 560 3.07e-139 416
Msa0035110 MtrunA17_Chr3g0116271 45.219 502 255 7 70 562 61 551 2.91e-134 402
Msa0035110 MtrunA17_Chr1g0180541 42.966 526 284 10 48 562 21 541 9.43e-132 395
Msa0035110 MtrunA17_Chr8g0353341 43.785 539 268 14 15 532 2 526 9.31e-129 387
Msa0035110 MtrunA17_Chr3g0116251 43.863 497 266 8 70 562 62 549 8.83e-127 383
Msa0035110 MtrunA17_Chr1g0180551 41.513 542 294 9 48 573 90 624 6.80e-125 380
Msa0035110 MtrunA17_Chr3g0102411 38.224 518 305 6 49 559 22 531 1.61e-115 354
Msa0035110 MtrunA17_Chr1g0186071 40.816 539 305 8 25 562 24 549 2.52e-115 353
Msa0035110 MtrunA17_Chr4g0072561 39.179 536 301 9 48 570 26 549 1.72e-110 341
Msa0035110 MtrunA17_Chr5g0395431 39.448 507 285 7 69 561 81 579 2.82e-103 323
Msa0035110 MtrunA17_Chr4g0000461 39.558 498 289 6 73 561 40 534 1.72e-101 317
Msa0035110 MtrunA17_Chr2g0334131 47.482 278 139 3 289 561 1 276 1.33e-80 254
Msa0035110 MtrunA17_Chr1g0157431 39.714 350 191 7 25 360 9 352 2.33e-64 219
Msa0035110 MtrunA17_Chr6g0471921 29.592 490 313 15 86 559 32 505 9.43e-38 146
Msa0035110 MtrunA17_Chr8g0370701 25.825 515 331 14 87 566 83 581 1.08e-36 144
Msa0035110 MtrunA17_Chr5g0433241 27.432 514 314 16 87 558 41 537 2.39e-36 142
Msa0035110 MtrunA17_Chr2g0301671 28.346 381 235 11 210 566 190 556 7.29e-35 138
Msa0035110 MtrunA17_Chr5g0396311 26.154 520 337 13 85 573 39 542 8.01e-34 135
Msa0035110 MtrunA17_Chr3g0109731 25.660 530 335 16 85 571 43 556 1.92e-33 134
Msa0035110 MtrunA17_Chr4g0068151 27.513 378 242 12 210 566 190 556 8.00e-31 126
Msa0035110 MtrunA17_Chr5g0396331 25.819 519 338 18 85 573 39 540 6.49e-30 124
Msa0035110 MtrunA17_Chr5g0433231 25.407 492 318 11 108 561 61 541 8.09e-30 124
Msa0035110 MtrunA17_Chr4g0028451 51.887 106 42 1 428 533 11 107 2.27e-29 112
Msa0035110 MtrunA17_Chr8g0370731 25.813 492 305 14 114 566 20 490 2.04e-28 119
Msa0035110 MtrunA17_Chr2g0292471 27.521 476 263 18 83 501 25 475 4.86e-26 112
Msa0035110 MtrunA17_Chr6g0471311 25.349 501 309 19 108 559 61 545 5.51e-26 112
Msa0035110 MtrunA17_Chr5g0433221 23.374 492 328 12 108 561 61 541 3.61e-24 106
Msa0035110 MtrunA17_Chr5g0406681 23.755 522 328 18 81 558 63 558 7.47e-23 102
Msa0035110 MtrunA17_Chr7g0270221 26.820 522 258 23 50 496 36 508 1.16e-19 92.8
Msa0035110 MtrunA17_Chr7g0252691 23.601 572 362 21 56 569 173 727 1.21e-19 93.2
Msa0035110 MtrunA17_Chr8g0388081 25.698 358 192 14 189 487 137 479 1.04e-17 86.7
Msa0035110 MtrunA17_Chr4g0004841 32.609 184 110 5 390 561 151 332 1.20e-17 84.7
Msa0035110 MtrunA17_Chr1g0194371 26.124 356 184 10 215 501 221 566 1.51e-17 86.3
Msa0035110 MtrunA17_Chr4g0004101 31.169 154 99 2 415 561 170 323 1.95e-17 84.0
Msa0035110 MtrunA17_Chr2g0329161 27.326 344 200 14 197 502 657 988 6.00e-14 75.5
Msa0035110 MtrunA17_Chr3g0121951 28.571 252 130 7 358 569 469 710 9.37e-14 74.3
Msa0035110 MtrunA17_Chr2g0334121 31.319 182 104 5 62 232 36 207 2.10e-13 69.7
Msa0035110 MtrunA17_Chr8g0351251 22.885 520 345 12 85 562 117 622 2.59e-13 73.2
Msa0035110 MtrunA17_Chr3g0121931 32.609 184 94 5 358 521 473 646 5.16e-13 72.0
Msa0035110 MtrunA17_Chr8g0377991 29.358 218 127 7 337 539 459 664 1.17e-12 70.9
Msa0035110 MtrunA17_Chr1g0160041 30.864 162 100 6 338 491 426 583 5.08e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0035110 AT1G20510.1 45.620 548 281 8 21 562 1 537 4.42e-156 458
Msa0035110 AT4G05160.1 43.013 551 297 6 27 569 3 544 3.30e-147 435
Msa0035110 AT1G20500.1 43.382 544 285 8 31 562 11 543 1.19e-146 434
Msa0035110 AT4G19010.1 43.438 541 286 9 30 561 25 554 1.20e-140 419
Msa0035110 AT5G38120.1 42.647 544 288 9 31 562 12 543 5.44e-138 412
Msa0035110 AT1G20480.1 42.831 551 286 12 25 561 22 557 2.36e-135 405
Msa0035110 AT5G63380.1 43.038 553 285 12 25 561 12 550 7.78e-135 404
Msa0035110 AT1G20510.2 43.595 484 256 8 21 498 1 473 5.51e-123 371
Msa0035110 AT5G38120.3 41.165 515 279 9 31 533 12 514 1.66e-120 366
Msa0035110 AT1G20510.3 41.991 462 251 8 21 476 1 451 4.59e-110 337
Msa0035110 AT1G65060.1 41.309 489 276 5 81 561 71 556 1.66e-108 336
Msa0035110 AT5G38120.4 40.417 480 262 9 31 498 12 479 2.90e-107 330
Msa0035110 AT5G38120.2 40.417 480 262 9 31 498 12 479 2.90e-107 330
Msa0035110 AT3G21240.1 39.370 508 277 8 73 561 51 546 7.09e-107 332
Msa0035110 AT1G62940.1 40.927 496 280 6 74 561 42 532 1.59e-104 325
Msa0035110 AT3G21230.1 40.354 508 286 6 68 561 56 560 5.54e-104 325
Msa0035110 AT1G51680.1 39.759 498 276 7 82 561 62 553 9.07e-98 308
Msa0035110 AT3G21230.3 39.583 480 273 6 68 533 56 532 1.10e-91 292
Msa0035110 AT1G51680.3 38.655 476 268 7 76 533 56 525 1.74e-85 276
Msa0035110 AT3G21240.2 38.215 437 253 8 73 497 51 482 2.72e-80 261
Msa0035110 AT3G21240.3 38.215 437 253 8 73 497 51 482 2.72e-80 261
Msa0035110 AT1G20490.2 37.123 431 244 10 31 446 11 429 1.58e-79 258
Msa0035110 AT1G20490.1 37.123 431 244 10 31 446 11 429 1.58e-79 258
Msa0035110 AT3G21230.2 39.101 445 254 6 67 497 55 496 2.10e-79 259
Msa0035110 AT1G65060.2 38.670 406 238 5 81 478 71 473 6.24e-74 244
Msa0035110 AT1G51680.2 37.955 440 249 7 76 497 56 489 2.14e-72 240
Msa0035110 AT3G21230.4 34.853 373 226 6 68 426 56 425 3.50e-44 163
Msa0035110 AT3G48990.1 30.061 489 314 12 85 559 30 504 2.33e-41 157
Msa0035110 AT1G65880.1 27.920 548 323 17 85 572 32 567 1.40e-39 152
Msa0035110 AT1G30520.2 27.387 555 328 16 74 571 21 557 1.10e-38 150
Msa0035110 AT1G30520.1 27.387 555 328 16 74 571 21 557 1.10e-38 150
Msa0035110 AT1G21540.1 27.660 517 320 15 82 558 37 539 4.24e-38 148
Msa0035110 AT1G65890.1 27.170 530 320 17 85 561 32 548 2.85e-37 146
Msa0035110 AT1G20560.1 26.628 522 332 15 87 573 43 548 4.35e-35 139
Msa0035110 AT1G77240.1 25.919 517 329 16 80 558 34 534 3.10e-34 137
Msa0035110 AT1G21530.2 25.869 518 327 14 81 558 38 538 3.65e-34 137
Msa0035110 AT1G21530.1 26.104 521 320 15 81 558 38 536 8.72e-34 135
Msa0035110 AT5G16370.1 25.294 510 336 14 82 558 37 534 4.47e-33 133
Msa0035110 AT1G66120.1 27.203 522 323 17 85 561 32 541 2.06e-32 132
Msa0035110 AT1G75960.1 25.882 510 329 13 87 561 42 537 1.16e-30 126
Msa0035110 AT1G20560.2 26.525 377 253 9 210 573 105 470 1.51e-30 125
Msa0035110 AT5G16340.1 24.361 509 342 13 82 558 37 534 5.11e-30 124
Msa0035110 AT1G30520.3 27.115 461 274 14 74 493 21 460 7.32e-30 123
Msa0035110 AT1G30520.4 27.115 461 274 14 74 493 21 460 7.32e-30 123
Msa0035110 AT3G16910.1 28.158 380 237 14 210 566 193 559 2.20e-29 122
Msa0035110 AT3G16170.1 26.649 379 233 11 215 561 235 600 2.61e-27 117
Msa0035110 AT2G17650.1 25.290 518 322 17 87 556 88 588 1.68e-24 108
Msa0035110 AT1G76290.1 24.288 527 340 18 76 562 23 530 2.08e-24 107
Msa0035110 AT1G68270.1 25.094 530 301 18 85 561 32 518 5.69e-24 106
Msa0035110 AT5G27600.1 25.160 469 263 20 86 484 119 569 2.54e-20 95.5
Msa0035110 AT5G36880.2 23.660 541 351 18 79 569 191 719 1.77e-17 86.7
Msa0035110 AT1G49430.1 26.479 355 191 14 199 496 210 551 2.08e-17 86.3
Msa0035110 AT5G36880.6 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0035110 AT5G36880.3 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0035110 AT5G36880.5 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0035110 AT5G36880.1 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0035110 AT2G47240.1 27.566 341 184 13 210 496 216 547 1.58e-16 83.6
Msa0035110 AT2G47240.2 27.566 341 184 13 210 496 216 547 1.58e-16 83.6
Msa0035110 AT2G47240.3 27.566 341 184 13 210 496 216 547 1.58e-16 83.6
Msa0035110 AT2G47240.4 27.566 341 184 13 210 496 157 488 1.61e-16 83.6
Msa0035110 AT1G77590.2 24.363 353 197 12 207 496 93 438 4.50e-16 81.6
Msa0035110 AT2G04350.2 24.576 354 185 15 207 491 268 608 4.22e-15 79.0
Msa0035110 AT2G04350.1 24.576 354 185 15 207 491 268 608 4.22e-15 79.0
Msa0035110 AT5G35930.4 23.495 515 335 16 87 556 59 559 5.25e-11 66.2

Find 146 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAATGTTTCATGTCTA+TGG 0.176321 1_1:+63154508 Msa0035110:CDS
ACCCTTCTCACTTTCTTATA+TGG 0.212821 1_1:-63155915 None:intergenic
ATCAGCTTTCCACATGCTTC+TGG 0.219682 1_1:-63154874 None:intergenic
AAATAAAGCTATGGTGTAAT+TGG 0.225021 1_1:+63156162 Msa0035110:three_prime_UTR
GGACTGATGTTCTTTGGATT+TGG 0.232726 1_1:+63154529 Msa0035110:CDS
GAATGGTAAATTCCTGTTCT+TGG 0.248189 1_1:-63153812 None:intergenic
GTCTATGGACTGATGTTCTT+TGG 0.250992 1_1:+63154523 Msa0035110:CDS
GTTGAGTACAAGATTGAGTT+TGG 0.273727 1_1:-63154073 None:intergenic
TAACTGCTGCATCAACAATA+AGG 0.276654 1_1:-63155343 None:intergenic
GCCGCTATACTTTACTCTTC+AGG 0.280862 1_1:+63154367 Msa0035110:CDS
CCAGCAGAGCAGTTTCATTA+TGG 0.284701 1_1:-63154440 None:intergenic
ACCAATTTGTGTAACTCTTC+TGG 0.296211 1_1:-63154214 None:intergenic
GACTGATGTTCTTTGGATTT+GGG 0.298826 1_1:+63154530 Msa0035110:CDS
CTTTGTTGGGATTTCATGTT+TGG 0.301287 1_1:-63153852 None:intergenic
CTCTGTCTCCATTTGATAAC+AGG 0.330041 1_1:+63153777 Msa0035110:CDS
TTGTATAAGTATTGAGGTTA+AGG 0.339381 1_1:-63153550 None:intergenic
CATGTCATTCAATTTGTTGC+AGG 0.341335 1_1:+63155524 Msa0035110:CDS
CACCATAGCTTTATTTCTTT+AGG 0.341674 1_1:-63156155 None:intergenic
AATCAAGCACAAAGGGTATC+AGG 0.343261 1_1:+63155122 Msa0035110:CDS
CTACGTTTCACTCTCTTTGC+CGG 0.344130 1_1:+63153487 Msa0035110:five_prime_UTR
AAATGTCAGGAGAGGAGATT+TGG 0.351734 1_1:+63153726 Msa0035110:CDS
AAAGTGATAGACATTGAAAC+AGG 0.356914 1_1:+63154913 Msa0035110:CDS
CTACATAGTTGAACGAATAA+AGG 0.367598 1_1:+63155095 Msa0035110:CDS
AACTGCTGCATCAACAATAA+GGG 0.374049 1_1:-63155342 None:intergenic
CTTGCAACAGCTTTGTTCCA+TGG 0.383330 1_1:+63154010 Msa0035110:CDS
TCAGGTAGCTCCTGCAGAAC+TGG 0.384556 1_1:+63155298 Msa0035110:intron
ACCAGAAGAGTTACACAAAT+TGG 0.386896 1_1:+63154213 Msa0035110:CDS
TCAGTTGAAGAGTTAATAGA+AGG 0.392562 1_1:+63154292 Msa0035110:CDS
AGGGTTGAAGATGAAGAAAC+TGG 0.395025 1_1:+63155446 Msa0035110:intron
CACCTGAGCCAACCCTTCTT+AGG 0.395579 1_1:-63154714 None:intergenic
GCGAAGCAAGTACATGATTC+AGG 0.402603 1_1:+63154169 Msa0035110:CDS
CTTTACTCTTCAGGGACTAC+TGG 0.405377 1_1:+63154376 Msa0035110:CDS
TATTTCCTTGGGTTGAACTA+AGG 0.410515 1_1:+63154779 Msa0035110:CDS
GTTCAGAAAGCTATTTCCTT+GGG 0.412932 1_1:+63154768 Msa0035110:CDS
TGTTGAGATCTGGCTTTGTT+GGG 0.419272 1_1:-63153865 None:intergenic
TCAATTTGTCAGTAAAACTT+TGG 0.420873 1_1:+63156233 Msa0035110:three_prime_UTR
CCGCTATACTTTACTCTTCA+GGG 0.425254 1_1:+63154368 Msa0035110:CDS
GTTGAACTAAGGGCGGGTTA+TGG 0.435071 1_1:+63154790 Msa0035110:CDS
CCATAATGAAACTGCTCTGC+TGG 0.435438 1_1:+63154440 Msa0035110:CDS
GTTCCTACAATTCTCACCTC+TGG 0.439013 1_1:+63154247 Msa0035110:CDS
GTGTTGAGATCTGGCTTTGT+TGG 0.439500 1_1:-63153866 None:intergenic
AGCGGCTACGATCCAAGAAC+AGG 0.444190 1_1:+63153800 Msa0035110:CDS
CATTCAATTTGTTGCAGGCC+AGG 0.446682 1_1:+63155529 Msa0035110:CDS
GGATTTGGGTTGTTGTGTGT+TGG 0.451406 1_1:+63154544 Msa0035110:CDS
TTTCTAAGTTGTGAGTAACT+TGG 0.452000 1_1:+63156021 Msa0035110:exon
TTTCAAGCTTAACAAGAGAA+TGG 0.452313 1_1:-63153829 None:intergenic
TTCACCAATGAGTGGATAAC+TGG 0.452438 1_1:-63154658 None:intergenic
GCTATTATGAAAGAATATCT+AGG 0.453902 1_1:+63154985 Msa0035110:CDS
GTGATTTGTCTAGCCTAAGA+AGG 0.457731 1_1:+63154701 Msa0035110:CDS
AGCACAGATTCCAGTTCTGC+AGG 0.460066 1_1:-63155308 None:intergenic
TGGATAACTGGTGGCACTGC+TGG 0.462633 1_1:-63154646 None:intergenic
AGAGTTGCAGTGTTGAGATC+TGG 0.462977 1_1:-63153875 None:intergenic
CTCACCTCTGGGACATCTGA+TGG 0.463000 1_1:+63154259 Msa0035110:CDS
GTCCCAGAGGTGAGAATTGT+AGG 0.467301 1_1:-63154250 None:intergenic
CATGGACTTGAGATTAGAAA+AGG 0.469403 1_1:+63154028 Msa0035110:CDS
TTACACAAATTGGTCCCAAC+TGG 0.471916 1_1:+63154223 Msa0035110:CDS
GCATATGTGGTGAGGGCAGC+TGG 0.475126 1_1:+63155482 Msa0035110:CDS
CAACAATTGATTCAGAAGGT+TGG 0.475182 1_1:+63155028 Msa0035110:CDS
TGTCAGTTGTATAAGTATTG+AGG 0.478516 1_1:-63153556 None:intergenic
TTCTAAGTTGTGAGTAACTT+GGG 0.479667 1_1:+63156022 Msa0035110:exon
TAGCCGCTCCTGTTATCAAA+TGG 0.484875 1_1:-63153785 None:intergenic
TGGTTGAGGACAGGTGATCT+TGG 0.485306 1_1:+63155048 Msa0035110:CDS
GCAGTGTCTGATTGCTCCAC+AGG 0.486700 1_1:-63154346 None:intergenic
TGATTTAGTATTGAGAGGGT+TGG 0.488156 1_1:-63154140 None:intergenic
GACATGAAACATTGGAATGA+AGG 0.489639 1_1:-63154503 None:intergenic
AGTTCAGAAAGCTATTTCCT+TGG 0.490058 1_1:+63154767 Msa0035110:CDS
AGGTGCTGCACCTTTGAGTA+AGG 0.492711 1_1:+63154732 Msa0035110:CDS
ATTTCCTTGGGTTGAACTAA+GGG 0.492805 1_1:+63154780 Msa0035110:CDS
CCTTCCTTGAGTGGAGGCAA+AGG 0.493651 1_1:-63154940 None:intergenic
CTTGGTTATATCGATGAGAA+TGG 0.496399 1_1:+63155066 Msa0035110:CDS
TTCACCAGATTCTTCTTCAA+CGG 0.499514 1_1:-63153753 None:intergenic
TAACTCTTCTGGTGCTGAGA+TGG 0.504393 1_1:-63154203 None:intergenic
ATGGACTAACAGAAAGTTGT+GGG 0.505501 1_1:+63154809 Msa0035110:CDS
AGCCTAAGAAGGGTTGGCTC+AGG 0.509742 1_1:+63154712 Msa0035110:CDS
AGGAGCTAATCAAGCACAAA+GGG 0.511589 1_1:+63155115 Msa0035110:CDS
CATCAGCAAGATCTCCTTGT+GGG 0.511833 1_1:-63153913 None:intergenic
CACTCAAGGAAGGAGAGTTA+TGG 0.515549 1_1:+63154950 Msa0035110:CDS
TCATCCGTTGAAGAAGAATC+TGG 0.516179 1_1:+63153749 Msa0035110:CDS
GCCACCAGTTATCCACTCAT+TGG 0.516190 1_1:+63154654 Msa0035110:CDS
TGTTTAGCTGTGTTATCAGT+TGG 0.521525 1_1:+63154100 Msa0035110:CDS
GTGAAACATGCTAGCAAAGA+TGG 0.522574 1_1:+63154676 Msa0035110:CDS
TTGATTCAGAAGGTTGGTTG+AGG 0.524384 1_1:+63155034 Msa0035110:CDS
TCAGAAGGTTGGTTGAGGAC+AGG 0.530736 1_1:+63155039 Msa0035110:CDS
TTTCTGATTTAGTATTGAGA+GGG 0.531374 1_1:-63154144 None:intergenic
GAAGCCTTTGCCTCCACTCA+AGG 0.535824 1_1:+63154936 Msa0035110:CDS
TTCCTACAATTCTCACCTCT+GGG 0.539856 1_1:+63154248 Msa0035110:CDS
ACTAATCATAGTGTAACTTA+CGG 0.545558 1_1:+63153962 Msa0035110:CDS
CATGGAACAAAGCTGTTGCA+AGG 0.549110 1_1:-63154009 None:intergenic
CCTTGGGTTGAACTAAGGGC+GGG 0.553742 1_1:+63154784 Msa0035110:CDS
TCCTTGGGTTGAACTAAGGG+CGG 0.554693 1_1:+63154783 Msa0035110:CDS
AGAATTGTAGGAACTCCAGT+TGG 0.555282 1_1:-63154238 None:intergenic
AGTATACCGAGGTCAGCTGC+TGG 0.555861 1_1:+63155951 Msa0035110:CDS
CATAACTCTCCTTCCTTGAG+TGG 0.558671 1_1:-63154949 None:intergenic
TTTGAGTAAGGAAATGTCAC+TGG 0.559342 1_1:+63154744 Msa0035110:CDS
TGGTTTAGTATAAACTGAGT+TGG 0.560230 1_1:+63156253 Msa0035110:three_prime_UTR
ACCAATGGCATATGTGGTGA+GGG 0.561041 1_1:+63155475 Msa0035110:CDS
AGCCTAAAGAAATAAAGCTA+TGG 0.561824 1_1:+63156153 Msa0035110:three_prime_UTR
GCCCTCACCACATATGCCAT+TGG 0.565672 1_1:-63155476 None:intergenic
ATTTCTGATTTAGTATTGAG+AGG 0.569356 1_1:-63154145 None:intergenic
TTGTCTAGCCTAAGAAGGGT+TGG 0.570054 1_1:+63154706 Msa0035110:CDS
GAATTGTAGGAACTCCAGTT+GGG 0.571691 1_1:-63154237 None:intergenic
AGAATATCTAGGAAATATTG+AGG 0.571721 1_1:+63154996 Msa0035110:CDS
CTCACAACTTAGAAACAAGC+TGG 0.574328 1_1:-63156013 None:intergenic
AGAAGCCGCCTTATATCAGA+AGG 0.579500 1_1:+63156112 Msa0035110:three_prime_UTR
CCTTTGCCTCCACTCAAGGA+AGG 0.582843 1_1:+63154940 Msa0035110:CDS
AGTATTGAGAGGGTTGGCTG+TGG 0.583867 1_1:-63154134 None:intergenic
TGATTTGTCTAGCCTAAGAA+GGG 0.587869 1_1:+63154702 Msa0035110:CDS
ATCTTGCCAGCAGCTGACCT+CGG 0.591787 1_1:-63155957 None:intergenic
TCCATATAAGAAAGTGAGAA+GGG 0.594862 1_1:+63155914 Msa0035110:CDS
CTCCATATAAGAAAGTGAGA+AGG 0.595321 1_1:+63155913 Msa0035110:CDS
TACCAATGGCATATGTGGTG+AGG 0.595660 1_1:+63155474 Msa0035110:CDS
ACTGCAACAATTGATTCAGA+AGG 0.598082 1_1:+63155024 Msa0035110:CDS
ACTGCTGCATCAACAATAAG+GGG 0.600356 1_1:-63155341 None:intergenic
AGTGACATTTCCTTACTCAA+AGG 0.602475 1_1:-63154742 None:intergenic
AACTCTCCTTCCTTGAGTGG+AGG 0.608300 1_1:-63154946 None:intergenic
GCAGATACCAATGGCATATG+TGG 0.613364 1_1:+63155469 Msa0035110:CDS
AGTCCATAGACATGAAACAT+TGG 0.615670 1_1:-63154511 None:intergenic
AAGGAGCTAATCAAGCACAA+AGG 0.617428 1_1:+63155114 Msa0035110:CDS
TTACCCCTTCTGATATAAGG+CGG 0.622594 1_1:-63156117 None:intergenic
ATGGAACAAAGCTGTTGCAA+GGG 0.623142 1_1:-63154008 None:intergenic
GATTGCTCCACAGGAACATG+TGG 0.623243 1_1:-63154337 None:intergenic
CCCGCCCTTAGTTCAACCCA+AGG 0.635164 1_1:-63154784 None:intergenic
GGGACTACTGGAGTAAGCAA+AGG 0.641617 1_1:+63154388 Msa0035110:CDS
GTGCTATCACAGTTCCCACA+AGG 0.647014 1_1:+63153899 Msa0035110:CDS
CCCTGAAGAGTAAAGTATAG+CGG 0.649066 1_1:-63154368 None:intergenic
ACCAATGAGTGGATAACTGG+TGG 0.649415 1_1:-63154655 None:intergenic
GGGTTGAAGATGAAGAAACT+GGG 0.650548 1_1:+63155447 Msa0035110:intron
AGAATTGCCACATGTTCCTG+TGG 0.656792 1_1:+63154330 Msa0035110:CDS
AAAGCTCATCCAGAAGCATG+TGG 0.657133 1_1:+63154865 Msa0035110:CDS
GAAACATTGGAATGAAGGCG+AGG 0.657962 1_1:-63154498 None:intergenic
AAGCCGCCTTATATCAGAAG+GGG 0.658392 1_1:+63156114 Msa0035110:three_prime_UTR
AGAAACTGGGCAGATACCAA+TGG 0.659161 1_1:+63155460 Msa0035110:CDS
TATGGACTAACAGAAAGTTG+TGG 0.659761 1_1:+63154808 Msa0035110:CDS
CTGCAACAAATTGAATGACA+TGG 0.661163 1_1:-63155523 None:intergenic
TAGCATGTTTCACCAATGAG+TGG 0.661752 1_1:-63154666 None:intergenic
TCTCCATTTGATAACAGGAG+CGG 0.663420 1_1:+63153782 Msa0035110:CDS
CTTCTCGATAGCGACAAGCA+TGG 0.668123 1_1:-63154605 None:intergenic
GAAGCCGCCTTATATCAGAA+GGG 0.670001 1_1:+63156113 Msa0035110:three_prime_UTR
GCATCAGCAAGATCTCCTTG+TGG 0.671856 1_1:-63153914 None:intergenic
GGACTAACAGAAAGTTGTGG+GGG 0.674978 1_1:+63154811 Msa0035110:CDS
TGGACTAACAGAAAGTTGTG+GGG 0.694783 1_1:+63154810 Msa0035110:CDS
ATGAAGTGGAGTTCAAAGCG+AGG 0.696930 1_1:+63156089 Msa0035110:three_prime_UTR
GGAGAGTTATGGTTGAAAAG+CGG 0.697534 1_1:+63154961 Msa0035110:CDS
AGTAACTTGGGCATAAACGA+CGG 0.698899 1_1:+63156034 Msa0035110:exon
AAGTGATAGACATTGAAACA+GGG 0.705961 1_1:+63154914 Msa0035110:CDS
CTTTCCATCAGATGTCCCAG+AGG 0.738092 1_1:-63154263 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTATTAATACATATA+TGG + chr1_1:63155265-63155284 Msa0035110:intron 10.0%
!!! AAAATTTAACTTAAATAATC+AGG - chr1_1:63155672-63155691 None:intergenic 10.0%
!! AGTAAATTACTTTAATTCTA+AGG + chr1_1:63155216-63155235 Msa0035110:intron 15.0%
!!! TTTGTATTTTAAACCTAATT+AGG - chr1_1:63155715-63155734 None:intergenic 15.0%
!!! GATATTTTTCGTAAAATACT+AGG - chr1_1:63153599-63153618 None:intergenic 20.0%
!! ATTGAAACTGAAACTATTTA+TGG - chr1_1:63155157-63155176 None:intergenic 20.0%
!! ATAAATAGTTTCAGTTTCAA+TGG + chr1_1:63155156-63155175 Msa0035110:intron 20.0%
!! ATGAAAATAAGAGTGAAAAT+AGG - chr1_1:63155388-63155407 None:intergenic 20.0%
!! AGAGAAATTAAAATGACTAT+AGG - chr1_1:63155849-63155868 None:intergenic 20.0%
!!! AAATAAATCTGAGAATTTTC+AGG + chr1_1:63155887-63155906 Msa0035110:intron 20.0%
!!! TTTTTCTTCAATTAATGAAG+TGG + chr1_1:63156075-63156094 Msa0035110:three_prime_UTR 20.0%
!! TTTTTGTCCTGAAATTTTTG+TGG - chr1_1:63153513-63153532 None:intergenic 25.0%
!!! TTGTATAAGTATTGAGGTTA+AGG - chr1_1:63153553-63153572 None:intergenic 25.0%
!! AACATATCTATCAATTTTGC+AGG + chr1_1:63153699-63153718 Msa0035110:intron 25.0%
!! ACATATCTATCAATTTTGCA+GGG + chr1_1:63153700-63153719 Msa0035110:intron 25.0%
! ACTAATCATAGTGTAACTTA+CGG + chr1_1:63153962-63153981 Msa0035110:CDS 25.0%
!! TTTCTGATTTAGTATTGAGA+GGG - chr1_1:63154147-63154166 None:intergenic 25.0%
!! ATTTCTGATTTAGTATTGAG+AGG - chr1_1:63154148-63154167 None:intergenic 25.0%
! GCTATTATGAAAGAATATCT+AGG + chr1_1:63154985-63155004 Msa0035110:CDS 25.0%
! AGAATATCTAGGAAATATTG+AGG + chr1_1:63154996-63155015 Msa0035110:CDS 25.0%
! AAAATAAGAGTGAAAATAGG+CGG - chr1_1:63155385-63155404 None:intergenic 25.0%
!!! TTATTTTACTGCATGAAATC+AGG - chr1_1:63155571-63155590 None:intergenic 25.0%
! AAATAAAGCTATGGTGTAAT+TGG + chr1_1:63156162-63156181 Msa0035110:three_prime_UTR 25.0%
! TCAATTTGTCAGTAAAACTT+TGG + chr1_1:63156233-63156252 Msa0035110:three_prime_UTR 25.0%
! TGTCAGTTGTATAAGTATTG+AGG - chr1_1:63153559-63153578 None:intergenic 30.0%
CAGCATCTATAGTATATACA+AGG - chr1_1:63153621-63153640 None:intergenic 30.0%
TTTCAAGCTTAACAAGAGAA+TGG - chr1_1:63153832-63153851 None:intergenic 30.0%
TCAGTTGAAGAGTTAATAGA+AGG + chr1_1:63154292-63154311 Msa0035110:CDS 30.0%
ATTCCAATGTTTCATGTCTA+TGG + chr1_1:63154508-63154527 Msa0035110:CDS 30.0%
!! ATCACTTTTGCACAAAAAGT+TGG - chr1_1:63154901-63154920 None:intergenic 30.0%
AAAGTGATAGACATTGAAAC+AGG + chr1_1:63154913-63154932 Msa0035110:CDS 30.0%
AAGTGATAGACATTGAAACA+GGG + chr1_1:63154914-63154933 Msa0035110:CDS 30.0%
CTACATAGTTGAACGAATAA+AGG + chr1_1:63155095-63155114 Msa0035110:CDS 30.0%
! CTAAGGTATCAAATTGAGTT+TGG + chr1_1:63155233-63155252 Msa0035110:intron 30.0%
GTATCAAATTGAGTTTGGAA+CGG + chr1_1:63155238-63155257 Msa0035110:intron 30.0%
! TTGTAATCATTGTTGCTTAG+TGG + chr1_1:63155601-63155620 Msa0035110:intron 30.0%
TATTCTGACTTCACCTAATT+AGG + chr1_1:63155699-63155718 Msa0035110:intron 30.0%
ATTGTTGGATGAATGTCATA+TGG - chr1_1:63155751-63155770 None:intergenic 30.0%
AGTTATTGCATCTAGATTGT+TGG - chr1_1:63155766-63155785 None:intergenic 30.0%
!! TAAATCTGAGAATTTTCAGG+TGG + chr1_1:63155890-63155909 Msa0035110:intron 30.0%
TCCATATAAGAAAGTGAGAA+GGG + chr1_1:63155914-63155933 Msa0035110:CDS 30.0%
TTTCTAAGTTGTGAGTAACT+TGG + chr1_1:63156021-63156040 Msa0035110:exon 30.0%
TTCTAAGTTGTGAGTAACTT+GGG + chr1_1:63156022-63156041 Msa0035110:exon 30.0%
! ATTTTACCCCTTCTGATATA+AGG - chr1_1:63156123-63156142 None:intergenic 30.0%
AGCCTAAAGAAATAAAGCTA+TGG + chr1_1:63156153-63156172 Msa0035110:three_prime_UTR 30.0%
!! CACCATAGCTTTATTTCTTT+AGG - chr1_1:63156158-63156177 None:intergenic 30.0%
TGGTTTAGTATAAACTGAGT+TGG + chr1_1:63156253-63156272 Msa0035110:three_prime_UTR 30.0%
TTGTCAATGCTAATGTAGTG+TGG - chr1_1:63153672-63153691 None:intergenic 35.0%
! TTTTGCAGGGAAAAAATGTC+AGG + chr1_1:63153713-63153732 Msa0035110:intron 35.0%
TTCACCAGATTCTTCTTCAA+CGG - chr1_1:63153756-63153775 None:intergenic 35.0%
GAATGGTAAATTCCTGTTCT+TGG - chr1_1:63153815-63153834 None:intergenic 35.0%
CTTTGTTGGGATTTCATGTT+TGG - chr1_1:63153855-63153874 None:intergenic 35.0%
! CTTTTCTAATCTCAAGTCCA+TGG - chr1_1:63154030-63154049 None:intergenic 35.0%
CATGGACTTGAGATTAGAAA+AGG + chr1_1:63154028-63154047 Msa0035110:CDS 35.0%
GTTGAGTACAAGATTGAGTT+TGG - chr1_1:63154076-63154095 None:intergenic 35.0%
TGTTTAGCTGTGTTATCAGT+TGG + chr1_1:63154100-63154119 Msa0035110:CDS 35.0%
! TGATTTAGTATTGAGAGGGT+TGG - chr1_1:63154143-63154162 None:intergenic 35.0%
ACCAGAAGAGTTACACAAAT+TGG + chr1_1:63154213-63154232 Msa0035110:CDS 35.0%
ACCAATTTGTGTAACTCTTC+TGG - chr1_1:63154217-63154236 None:intergenic 35.0%
GACATGAAACATTGGAATGA+AGG - chr1_1:63154506-63154525 None:intergenic 35.0%
AGTCCATAGACATGAAACAT+TGG - chr1_1:63154514-63154533 None:intergenic 35.0%
GACTGATGTTCTTTGGATTT+GGG + chr1_1:63154530-63154549 Msa0035110:CDS 35.0%
! TGATTTGTCTAGCCTAAGAA+GGG + chr1_1:63154702-63154721 Msa0035110:CDS 35.0%
AGTGACATTTCCTTACTCAA+AGG - chr1_1:63154745-63154764 None:intergenic 35.0%
TTTGAGTAAGGAAATGTCAC+TGG + chr1_1:63154744-63154763 Msa0035110:CDS 35.0%
! AGTTCAGAAAGCTATTTCCT+TGG + chr1_1:63154767-63154786 Msa0035110:CDS 35.0%
! GTTCAGAAAGCTATTTCCTT+GGG + chr1_1:63154768-63154787 Msa0035110:CDS 35.0%
!! TATTTCCTTGGGTTGAACTA+AGG + chr1_1:63154779-63154798 Msa0035110:CDS 35.0%
!! ATTTCCTTGGGTTGAACTAA+GGG + chr1_1:63154780-63154799 Msa0035110:CDS 35.0%
! TATGGACTAACAGAAAGTTG+TGG + chr1_1:63154808-63154827 Msa0035110:CDS 35.0%
! ATGGACTAACAGAAAGTTGT+GGG + chr1_1:63154809-63154828 Msa0035110:CDS 35.0%
! ACTGCAACAATTGATTCAGA+AGG + chr1_1:63155024-63155043 Msa0035110:CDS 35.0%
! CAACAATTGATTCAGAAGGT+TGG + chr1_1:63155028-63155047 Msa0035110:CDS 35.0%
CTTGGTTATATCGATGAGAA+TGG + chr1_1:63155066-63155085 Msa0035110:CDS 35.0%
! ATATATGGATTGCTTGTGTC+AGG + chr1_1:63155280-63155299 Msa0035110:intron 35.0%
AACTGCTGCATCAACAATAA+GGG - chr1_1:63155345-63155364 None:intergenic 35.0%
TAACTGCTGCATCAACAATA+AGG - chr1_1:63155346-63155365 None:intergenic 35.0%
ATGTGGACAAAAAATGTTGC+AGG + chr1_1:63155426-63155445 Msa0035110:intron 35.0%
TGTGGACAAAAAATGTTGCA+GGG + chr1_1:63155427-63155446 Msa0035110:intron 35.0%
CTGCAACAAATTGAATGACA+TGG - chr1_1:63155526-63155545 None:intergenic 35.0%
!! CATGTCATTCAATTTGTTGC+AGG + chr1_1:63155524-63155543 Msa0035110:CDS 35.0%
! AATCATTGTTGCTTAGTGGT+TGG + chr1_1:63155605-63155624 Msa0035110:intron 35.0%
CTCCATATAAGAAAGTGAGA+AGG + chr1_1:63155913-63155932 Msa0035110:CDS 35.0%
ACCCTTCTCACTTTCTTATA+TGG - chr1_1:63155918-63155937 None:intergenic 35.0%
!! GTTTTATTGACAGTATACCG+AGG + chr1_1:63155940-63155959 Msa0035110:CDS 35.0%
! TGTCCTGAAATTTTTGTGGC+CGG - chr1_1:63153509-63153528 None:intergenic 40.0%
AAATGTCAGGAGAGGAGATT+TGG + chr1_1:63153726-63153745 Msa0035110:CDS 40.0%
TCATCCGTTGAAGAAGAATC+TGG + chr1_1:63153749-63153768 Msa0035110:CDS 40.0%
! CTCTGTCTCCATTTGATAAC+AGG + chr1_1:63153777-63153796 Msa0035110:CDS 40.0%
! TCTCCATTTGATAACAGGAG+CGG + chr1_1:63153782-63153801 Msa0035110:CDS 40.0%
!! TGTTGAGATCTGGCTTTGTT+GGG - chr1_1:63153868-63153887 None:intergenic 40.0%
ATGGAACAAAGCTGTTGCAA+GGG - chr1_1:63154011-63154030 None:intergenic 40.0%
TTACACAAATTGGTCCCAAC+TGG + chr1_1:63154223-63154242 Msa0035110:CDS 40.0%
GAATTGTAGGAACTCCAGTT+GGG - chr1_1:63154240-63154259 None:intergenic 40.0%
AGAATTGTAGGAACTCCAGT+TGG - chr1_1:63154241-63154260 None:intergenic 40.0%
TTCCTACAATTCTCACCTCT+GGG + chr1_1:63154248-63154267 Msa0035110:CDS 40.0%
CCCTGAAGAGTAAAGTATAG+CGG - chr1_1:63154371-63154390 None:intergenic 40.0%
CCGCTATACTTTACTCTTCA+GGG + chr1_1:63154368-63154387 Msa0035110:CDS 40.0%
! GTCTATGGACTGATGTTCTT+TGG + chr1_1:63154523-63154542 Msa0035110:CDS 40.0%
GGACTGATGTTCTTTGGATT+TGG + chr1_1:63154529-63154548 Msa0035110:CDS 40.0%
TTCACCAATGAGTGGATAAC+TGG - chr1_1:63154661-63154680 None:intergenic 40.0%
TAGCATGTTTCACCAATGAG+TGG - chr1_1:63154669-63154688 None:intergenic 40.0%
GTGAAACATGCTAGCAAAGA+TGG + chr1_1:63154676-63154695 Msa0035110:CDS 40.0%
! GTGATTTGTCTAGCCTAAGA+AGG + chr1_1:63154701-63154720 Msa0035110:CDS 40.0%
TGGACTAACAGAAAGTTGTG+GGG + chr1_1:63154810-63154829 Msa0035110:CDS 40.0%
GGAGAGTTATGGTTGAAAAG+CGG + chr1_1:63154961-63154980 Msa0035110:CDS 40.0%
! TTGATTCAGAAGGTTGGTTG+AGG + chr1_1:63155034-63155053 Msa0035110:CDS 40.0%
AAGGAGCTAATCAAGCACAA+AGG + chr1_1:63155114-63155133 Msa0035110:CDS 40.0%
AGGAGCTAATCAAGCACAAA+GGG + chr1_1:63155115-63155134 Msa0035110:CDS 40.0%
!! AATCAAGCACAAAGGGTATC+AGG + chr1_1:63155122-63155141 Msa0035110:CDS 40.0%
ACTGCTGCATCAACAATAAG+GGG - chr1_1:63155344-63155363 None:intergenic 40.0%
TAAGAGTGAAAATAGGCGGA+GGG - chr1_1:63155381-63155400 None:intergenic 40.0%
ATAAGAGTGAAAATAGGCGG+AGG - chr1_1:63155382-63155401 None:intergenic 40.0%
CTCTCTGTGACTTTCTTATG+TGG + chr1_1:63155409-63155428 Msa0035110:intron 40.0%
AGGGTTGAAGATGAAGAAAC+TGG + chr1_1:63155446-63155465 Msa0035110:intron 40.0%
GGGTTGAAGATGAAGAAACT+GGG + chr1_1:63155447-63155466 Msa0035110:intron 40.0%
CTATAGGAAAACGAGAAAGC+AGG - chr1_1:63155833-63155852 None:intergenic 40.0%
CTCACAACTTAGAAACAAGC+TGG - chr1_1:63156016-63156035 None:intergenic 40.0%
AGTAACTTGGGCATAAACGA+CGG + chr1_1:63156034-63156053 Msa0035110:exon 40.0%
TTACCCCTTCTGATATAAGG+CGG - chr1_1:63156120-63156139 None:intergenic 40.0%
CTACGTTTCACTCTCTTTGC+CGG + chr1_1:63153487-63153506 Msa0035110:five_prime_UTR 45.0%
TTGCCGGCCACAAAAATTTC+AGG + chr1_1:63153503-63153522 Msa0035110:five_prime_UTR 45.0%
ATGCTAATGTAGTGTGGCTG+TGG - chr1_1:63153666-63153685 None:intergenic 45.0%
CAGGGAAAAAATGTCAGGAG+AGG + chr1_1:63153718-63153737 Msa0035110:intron 45.0%
TAGCCGCTCCTGTTATCAAA+TGG - chr1_1:63153788-63153807 None:intergenic 45.0%
!! GTGTTGAGATCTGGCTTTGT+TGG - chr1_1:63153869-63153888 None:intergenic 45.0%
! AGAGTTGCAGTGTTGAGATC+TGG - chr1_1:63153878-63153897 None:intergenic 45.0%
CATCAGCAAGATCTCCTTGT+GGG - chr1_1:63153916-63153935 None:intergenic 45.0%
CATGGAACAAAGCTGTTGCA+AGG - chr1_1:63154012-63154031 None:intergenic 45.0%
!! CTTGCAACAGCTTTGTTCCA+TGG + chr1_1:63154010-63154029 Msa0035110:CDS 45.0%
GCGAAGCAAGTACATGATTC+AGG + chr1_1:63154169-63154188 Msa0035110:CDS 45.0%
! TAACTCTTCTGGTGCTGAGA+TGG - chr1_1:63154206-63154225 None:intergenic 45.0%
GTTCCTACAATTCTCACCTC+TGG + chr1_1:63154247-63154266 Msa0035110:CDS 45.0%
AGAATTGCCACATGTTCCTG+TGG + chr1_1:63154330-63154349 Msa0035110:CDS 45.0%
GCCGCTATACTTTACTCTTC+AGG + chr1_1:63154367-63154386 Msa0035110:CDS 45.0%
CTTTACTCTTCAGGGACTAC+TGG + chr1_1:63154376-63154395 Msa0035110:CDS 45.0%
CCAGCAGAGCAGTTTCATTA+TGG - chr1_1:63154443-63154462 None:intergenic 45.0%
CCATAATGAAACTGCTCTGC+TGG + chr1_1:63154440-63154459 Msa0035110:CDS 45.0%
GAAACATTGGAATGAAGGCG+AGG - chr1_1:63154501-63154520 None:intergenic 45.0%
!! GGATTTGGGTTGTTGTGTGT+TGG + chr1_1:63154544-63154563 Msa0035110:CDS 45.0%
ACCAATGAGTGGATAACTGG+TGG - chr1_1:63154658-63154677 None:intergenic 45.0%
TTGTCTAGCCTAAGAAGGGT+TGG + chr1_1:63154706-63154725 Msa0035110:CDS 45.0%
GGACTAACAGAAAGTTGTGG+GGG + chr1_1:63154811-63154830 Msa0035110:CDS 45.0%
! AAAGCTCATCCAGAAGCATG+TGG + chr1_1:63154865-63154884 Msa0035110:CDS 45.0%
ATCAGCTTTCCACATGCTTC+TGG - chr1_1:63154877-63154896 None:intergenic 45.0%
CATAACTCTCCTTCCTTGAG+TGG - chr1_1:63154952-63154971 None:intergenic 45.0%
CACTCAAGGAAGGAGAGTTA+TGG + chr1_1:63154950-63154969 Msa0035110:CDS 45.0%
AAGAGTGAAAATAGGCGGAG+GGG - chr1_1:63155380-63155399 None:intergenic 45.0%
AGAAACTGGGCAGATACCAA+TGG + chr1_1:63155460-63155479 Msa0035110:CDS 45.0%
GCAGATACCAATGGCATATG+TGG + chr1_1:63155469-63155488 Msa0035110:CDS 45.0%
TACCAATGGCATATGTGGTG+AGG + chr1_1:63155474-63155493 Msa0035110:CDS 45.0%
ACCAATGGCATATGTGGTGA+GGG + chr1_1:63155475-63155494 Msa0035110:CDS 45.0%
!! CATTCAATTTGTTGCAGGCC+AGG + chr1_1:63155529-63155548 Msa0035110:CDS 45.0%
ATGAAATCAGGAGCCAAGAG+TGG - chr1_1:63155559-63155578 None:intergenic 45.0%
GGAGCTAAGAGAAGCAGAAA+TGG - chr1_1:63155812-63155831 None:intergenic 45.0%
ATGAAGTGGAGTTCAAAGCG+AGG + chr1_1:63156089-63156108 Msa0035110:three_prime_UTR 45.0%
AGAAGCCGCCTTATATCAGA+AGG + chr1_1:63156112-63156131 Msa0035110:three_prime_UTR 45.0%
GAAGCCGCCTTATATCAGAA+GGG + chr1_1:63156113-63156132 Msa0035110:three_prime_UTR 45.0%
AAGCCGCCTTATATCAGAAG+GGG + chr1_1:63156114-63156133 Msa0035110:three_prime_UTR 45.0%
GTGCTATCACAGTTCCCACA+AGG + chr1_1:63153899-63153918 Msa0035110:CDS 50.0%
GCATCAGCAAGATCTCCTTG+TGG - chr1_1:63153917-63153936 None:intergenic 50.0%
!! AGTATTGAGAGGGTTGGCTG+TGG - chr1_1:63154137-63154156 None:intergenic 50.0%
GTCCCAGAGGTGAGAATTGT+AGG - chr1_1:63154253-63154272 None:intergenic 50.0%
CTTTCCATCAGATGTCCCAG+AGG - chr1_1:63154266-63154285 None:intergenic 50.0%
GATTGCTCCACAGGAACATG+TGG - chr1_1:63154340-63154359 None:intergenic 50.0%
GGGACTACTGGAGTAAGCAA+AGG + chr1_1:63154388-63154407 Msa0035110:CDS 50.0%
CTTCTCGATAGCGACAAGCA+TGG - chr1_1:63154608-63154627 None:intergenic 50.0%
GCCACCAGTTATCCACTCAT+TGG + chr1_1:63154654-63154673 Msa0035110:CDS 50.0%
! AGGTGCTGCACCTTTGAGTA+AGG + chr1_1:63154732-63154751 Msa0035110:CDS 50.0%
!! TCCTTGGGTTGAACTAAGGG+CGG + chr1_1:63154783-63154802 Msa0035110:CDS 50.0%
!! GTTGAACTAAGGGCGGGTTA+TGG + chr1_1:63154790-63154809 Msa0035110:CDS 50.0%
! TGTGGGGGAGCTACGTTTTT+CGG + chr1_1:63154826-63154845 Msa0035110:CDS 50.0%
AACTCTCCTTCCTTGAGTGG+AGG - chr1_1:63154949-63154968 None:intergenic 50.0%
! TCAGAAGGTTGGTTGAGGAC+AGG + chr1_1:63155039-63155058 Msa0035110:CDS 50.0%
TGGTTGAGGACAGGTGATCT+TGG + chr1_1:63155048-63155067 Msa0035110:CDS 50.0%
AGCACAGATTCCAGTTCTGC+AGG - chr1_1:63155311-63155330 None:intergenic 50.0%
!! CAATTTGTTGCAGGCCAGGT+AGG + chr1_1:63155533-63155552 Msa0035110:intron 50.0%
AGCGGCTACGATCCAAGAAC+AGG + chr1_1:63153800-63153819 Msa0035110:CDS 55.0%
!! CTCACCTCTGGGACATCTGA+TGG + chr1_1:63154259-63154278 Msa0035110:CDS 55.0%
GCAGTGTCTGATTGCTCCAC+AGG - chr1_1:63154349-63154368 None:intergenic 55.0%
TGGATAACTGGTGGCACTGC+TGG - chr1_1:63154649-63154668 None:intergenic 55.0%
!! AGCCTAAGAAGGGTTGGCTC+AGG + chr1_1:63154712-63154731 Msa0035110:CDS 55.0%
CACCTGAGCCAACCCTTCTT+AGG - chr1_1:63154717-63154736 None:intergenic 55.0%
!! CCTTGGGTTGAACTAAGGGC+GGG + chr1_1:63154784-63154803 Msa0035110:CDS 55.0%
! GTGGGGGAGCTACGTTTTTC+GGG + chr1_1:63154827-63154846 Msa0035110:CDS 55.0%
!!! GGGAGCTACGTTTTTCGGGT+CGG + chr1_1:63154831-63154850 Msa0035110:CDS 55.0%
GAAGCCTTTGCCTCCACTCA+AGG + chr1_1:63154936-63154955 Msa0035110:CDS 55.0%
CCTTCCTTGAGTGGAGGCAA+AGG - chr1_1:63154943-63154962 None:intergenic 55.0%
CCTTTGCCTCCACTCAAGGA+AGG + chr1_1:63154940-63154959 Msa0035110:CDS 55.0%
TCAGGTAGCTCCTGCAGAAC+TGG + chr1_1:63155298-63155317 Msa0035110:intron 55.0%
AATAGGCGGAGGGGATCGTA+CGG - chr1_1:63155371-63155390 None:intergenic 55.0%
GCCCTCACCACATATGCCAT+TGG - chr1_1:63155479-63155498 None:intergenic 55.0%
AGTATACCGAGGTCAGCTGC+TGG + chr1_1:63155951-63155970 Msa0035110:CDS 55.0%
ATCTTGCCAGCAGCTGACCT+CGG - chr1_1:63155960-63155979 None:intergenic 55.0%
CCCGCCCTTAGTTCAACCCA+AGG - chr1_1:63154787-63154806 None:intergenic 60.0%
GCATATGTGGTGAGGGCAGC+TGG + chr1_1:63155482-63155501 Msa0035110:CDS 60.0%
CAGGCCAGGTAGGCCACTCT+TGG + chr1_1:63155543-63155562 Msa0035110:intron 65.0%
GGAGCCAAGAGTGGCCTACC+TGG - chr1_1:63155550-63155569 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 63153468 63156285 63153468 ID=Msa0035110;Name=Msa0035110
chr1_1 mRNA 63153468 63156285 63153468 ID=Msa0035110-mRNA-1;Parent=Msa0035110;Name=Msa0035110-mRNA-1;_AED=0.07;_eAED=0.07;_QI=120|1|1|1|0.75|0.8|5|251|573
chr1_1 exon 63153468 63153589 63153468 ID=Msa0035110-mRNA-1:exon:20047;Parent=Msa0035110-mRNA-1
chr1_1 exon 63153721 63155143 63153721 ID=Msa0035110-mRNA-1:exon:20048;Parent=Msa0035110-mRNA-1
chr1_1 exon 63155302 63155369 63155302 ID=Msa0035110-mRNA-1:exon:20049;Parent=Msa0035110-mRNA-1
chr1_1 exon 63155448 63155550 63155448 ID=Msa0035110-mRNA-1:exon:20050;Parent=Msa0035110-mRNA-1
chr1_1 exon 63155909 63156285 63155909 ID=Msa0035110-mRNA-1:exon:20051;Parent=Msa0035110-mRNA-1
chr1_1 five_prime_UTR 63153468 63153587 63153468 ID=Msa0035110-mRNA-1:five_prime_utr;Parent=Msa0035110-mRNA-1
chr1_1 CDS 63153588 63153589 63153588 ID=Msa0035110-mRNA-1:cds;Parent=Msa0035110-mRNA-1
chr1_1 CDS 63153721 63155143 63153721 ID=Msa0035110-mRNA-1:cds;Parent=Msa0035110-mRNA-1
chr1_1 CDS 63155302 63155369 63155302 ID=Msa0035110-mRNA-1:cds;Parent=Msa0035110-mRNA-1
chr1_1 CDS 63155448 63155550 63155448 ID=Msa0035110-mRNA-1:cds;Parent=Msa0035110-mRNA-1
chr1_1 CDS 63155909 63156034 63155909 ID=Msa0035110-mRNA-1:cds;Parent=Msa0035110-mRNA-1
chr1_1 three_prime_UTR 63156035 63156285 63156035 ID=Msa0035110-mRNA-1:three_prime_utr;Parent=Msa0035110-mRNA-1
Gene Sequence

>Msa0035110

ATGGAAAAAATGTCAGGAGAGGAGATTTGGTCATCCGTTGAAGAAGAATCTGGTGAATCTCTGTCTCCATTTGATAACAGGAGCGGCTACGATCCAAGAACAGGAATTTACCATTCTCTTGTTAAGCTTGAAACCAAACATGAAATCCCAACAAAGCCAGATCTCAACACTGCAACTCTTGTGCTATCACAGTTCCCACAAGGAGATCTTGCTGATGCAAGAATTGCATTCATTGACTTAAATACTAATCATAGTGTAACTTACGGTGAGATTCGTCGATCAGTTTACTCCCTTGCAACAGCTTTGTTCCATGGACTTGAGATTAGAAAAGGCGACGTCGTGTTTTTATTGTCTCCAAACTCAATCTTGTACTCAACTATATGTTTAGCTGTGTTATCAGTTGGTGCAATTCTTACCACAGCCAACCCTCTCAATACTAAATCAGAAATCGCGAAGCAAGTACATGATTCAGGCGCTAAACTAGCCATCTCAGCACCAGAAGAGTTACACAAATTGGTCCCAACTGGAGTTCCTACAATTCTCACCTCTGGGACATCTGATGGAAAGTTTTTATCAGTTGAAGAGTTAATAGAAGGCTGCTATGATTCGCAAGAATTGCCACATGTTCCTGTGGAGCAATCAGACACTGCCGCTATACTTTACTCTTCAGGGACTACTGGAGTAAGCAAAGGTGTTGTTTTGACACATGCAAATCTCATAACCATAATGAAACTGCTCTGCTGGTCTGCTGATGTATCTACAGCACAAGACGATGTTTTCCTCGCCTTCATTCCAATGTTTCATGTCTATGGACTGATGTTCTTTGGATTTGGGTTGTTGTGTGTTGGTGTTACGACAGTTTTGATGCAAAAATATGATTTTCAAGCCATGCTTGTCGCTATCGAGAAGCACAAAGTTAATAACATACCAGCAGTGCCACCAGTTATCCACTCATTGGTGAAACATGCTAGCAAAGATGGCTGTGATTTGTCTAGCCTAAGAAGGGTTGGCTCAGGTGCTGCACCTTTGAGTAAGGAAATGTCACTGGAGTTCAGAAAGCTATTTCCTTGGGTTGAACTAAGGGCGGGTTATGGACTAACAGAAAGTTGTGGGGGAGCTACGTTTTTCGGGTCGGATAAAGATGCTAAAGCTCATCCAGAAGCATGTGGAAAGCTGATTCCAACTTTTTGTGCAAAAGTGATAGACATTGAAACAGGGAAGCCTTTGCCTCCACTCAAGGAAGGAGAGTTATGGTTGAAAAGCGGTGCTATTATGAAAGAATATCTAGGAAATATTGAGGCAACAACTGCAACAATTGATTCAGAAGGTTGGTTGAGGACAGGTGATCTTGGTTATATCGATGAGAATGGAATTGTCTACATAGTTGAACGAATAAAGGAGCTAATCAAGCACAAAGGGTATCAGGTAGCTCCTGCAGAACTGGAATCTGTGCTGCTAAGTCATCCCCTTATTGTTGATGCAGCAGTTATTCCGGTTGAAGATGAAGAAACTGGGCAGATACCAATGGCATATGTGGTGAGGGCAGCTGGTTCTCAACTATCAGAAGACCATGTCATTCAATTTGTTGCAGGCCAGGTGGCTCCATATAAGAAAGTGAGAAGGGTGAGTTTTATTGACAGTATACCGAGGTCAGCTGCTGGCAAGATATTGCGAAAAGATCTTGTTTTGCAAAGCAAATACCAGCTTGTTTCTAAGTTGTGA
Protein sequence

>Msa0035110

MEKMSGEEIWSSVEEESGESLSPFDNRSGYDPRTGIYHSLVKLETKHEIPTKPDLNTATLVLSQFPQGDLADARIAFIDLNTNHSVTYGEIRRSVYSLATALFHGLEIRKGDVVFLLSPNSILYSTICLAVLSVGAILTTANPLNTKSEIAKQVHDSGAKLAISAPEELHKLVPTGVPTILTSGTSDGKFLSVEELIEGCYDSQELPHVPVEQSDTAAILYSSGTTGVSKGVVLTHANLITIMKLLCWSADVSTAQDDVFLAFIPMFHVYGLMFFGFGLLCVGVTTVLMQKYDFQAMLVAIEKHKVNNIPAVPPVIHSLVKHASKDGCDLSSLRRVGSGAAPLSKEMSLEFRKLFPWVELRAGYGLTESCGGATFFGSDKDAKAHPEACGKLIPTFCAKVIDIETGKPLPPLKEGELWLKSGAIMKEYLGNIEATTATIDSEGWLRTGDLGYIDENGIVYIVERIKELIKHKGYQVAPAELESVLLSHPLIVDAAVIPVEDEETGQIPMAYVVRAAGSQLSEDHVIQFVAGQVAPYKKVRRVSFIDSIPRSAAGKILRKDLVLQSKYQLVSKL*