AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0175920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0175920 MtrunA17_Chr1g0197391 97.895 570 12 0 4 573 1 570 0.0 1144
Msa0175920 MtrunA17_Chr4g0028411 43.716 549 294 7 27 569 3 542 2.48e-152 448
Msa0175920 MtrunA17_Chr1g0157401 45.471 552 280 9 25 562 9 553 1.06e-151 447
Msa0175920 MtrunA17_Chr2g0327341 40.585 547 313 6 26 569 2 539 1.81e-144 428
Msa0175920 MtrunA17_Chr5g0409771 45.037 544 275 12 28 561 18 547 2.23e-142 423
Msa0175920 MtrunA17_Chr4g0039791 45.422 557 272 12 30 573 24 561 3.64e-142 422
Msa0175920 MtrunA17_Chr5g0409791 44.585 554 271 12 30 561 21 560 3.07e-139 416
Msa0175920 MtrunA17_Chr3g0116271 45.219 502 255 7 70 562 61 551 2.91e-134 402
Msa0175920 MtrunA17_Chr1g0180541 42.966 526 284 10 48 562 21 541 9.43e-132 395
Msa0175920 MtrunA17_Chr8g0353341 43.785 539 268 14 15 532 2 526 9.31e-129 387
Msa0175920 MtrunA17_Chr3g0116251 43.863 497 266 8 70 562 62 549 8.83e-127 383
Msa0175920 MtrunA17_Chr1g0180551 41.513 542 294 9 48 573 90 624 6.80e-125 380
Msa0175920 MtrunA17_Chr3g0102411 38.224 518 305 6 49 559 22 531 1.61e-115 354
Msa0175920 MtrunA17_Chr1g0186071 40.816 539 305 8 25 562 24 549 2.52e-115 353
Msa0175920 MtrunA17_Chr4g0072561 39.179 536 301 9 48 570 26 549 1.72e-110 341
Msa0175920 MtrunA17_Chr5g0395431 39.448 507 285 7 69 561 81 579 2.82e-103 323
Msa0175920 MtrunA17_Chr4g0000461 39.558 498 289 6 73 561 40 534 1.72e-101 317
Msa0175920 MtrunA17_Chr2g0334131 47.482 278 139 3 289 561 1 276 1.33e-80 254
Msa0175920 MtrunA17_Chr1g0157431 39.714 350 191 7 25 360 9 352 2.33e-64 219
Msa0175920 MtrunA17_Chr6g0471921 29.592 490 313 15 86 559 32 505 9.43e-38 146
Msa0175920 MtrunA17_Chr8g0370701 25.825 515 331 14 87 566 83 581 1.08e-36 144
Msa0175920 MtrunA17_Chr5g0433241 27.432 514 314 16 87 558 41 537 2.39e-36 142
Msa0175920 MtrunA17_Chr2g0301671 28.346 381 235 11 210 566 190 556 7.29e-35 138
Msa0175920 MtrunA17_Chr5g0396311 26.154 520 337 13 85 573 39 542 8.01e-34 135
Msa0175920 MtrunA17_Chr3g0109731 25.660 530 335 16 85 571 43 556 1.92e-33 134
Msa0175920 MtrunA17_Chr4g0068151 27.513 378 242 12 210 566 190 556 8.00e-31 126
Msa0175920 MtrunA17_Chr5g0396331 25.819 519 338 18 85 573 39 540 6.49e-30 124
Msa0175920 MtrunA17_Chr5g0433231 25.407 492 318 11 108 561 61 541 8.09e-30 124
Msa0175920 MtrunA17_Chr4g0028451 51.887 106 42 1 428 533 11 107 2.27e-29 112
Msa0175920 MtrunA17_Chr8g0370731 25.813 492 305 14 114 566 20 490 2.04e-28 119
Msa0175920 MtrunA17_Chr2g0292471 27.521 476 263 18 83 501 25 475 4.86e-26 112
Msa0175920 MtrunA17_Chr6g0471311 25.349 501 309 19 108 559 61 545 5.51e-26 112
Msa0175920 MtrunA17_Chr5g0433221 23.374 492 328 12 108 561 61 541 3.61e-24 106
Msa0175920 MtrunA17_Chr5g0406681 23.755 522 328 18 81 558 63 558 7.47e-23 102
Msa0175920 MtrunA17_Chr7g0270221 26.820 522 258 23 50 496 36 508 1.16e-19 92.8
Msa0175920 MtrunA17_Chr7g0252691 23.601 572 362 21 56 569 173 727 1.21e-19 93.2
Msa0175920 MtrunA17_Chr8g0388081 25.698 358 192 14 189 487 137 479 1.04e-17 86.7
Msa0175920 MtrunA17_Chr4g0004841 32.609 184 110 5 390 561 151 332 1.20e-17 84.7
Msa0175920 MtrunA17_Chr1g0194371 26.124 356 184 10 215 501 221 566 1.51e-17 86.3
Msa0175920 MtrunA17_Chr4g0004101 31.169 154 99 2 415 561 170 323 1.95e-17 84.0
Msa0175920 MtrunA17_Chr2g0329161 27.326 344 200 14 197 502 657 988 6.00e-14 75.5
Msa0175920 MtrunA17_Chr3g0121951 28.571 252 130 7 358 569 469 710 9.37e-14 74.3
Msa0175920 MtrunA17_Chr2g0334121 31.319 182 104 5 62 232 36 207 2.10e-13 69.7
Msa0175920 MtrunA17_Chr8g0351251 22.885 520 345 12 85 562 117 622 2.59e-13 73.2
Msa0175920 MtrunA17_Chr3g0121931 32.609 184 94 5 358 521 473 646 5.16e-13 72.0
Msa0175920 MtrunA17_Chr8g0377991 29.358 218 127 7 337 539 459 664 1.17e-12 70.9
Msa0175920 MtrunA17_Chr1g0160041 30.864 162 100 6 338 491 426 583 5.08e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0175920 AT1G20510.1 45.620 548 281 8 21 562 1 537 4.42e-156 458
Msa0175920 AT4G05160.1 43.013 551 297 6 27 569 3 544 3.30e-147 435
Msa0175920 AT1G20500.1 43.382 544 285 8 31 562 11 543 1.19e-146 434
Msa0175920 AT4G19010.1 43.438 541 286 9 30 561 25 554 1.20e-140 419
Msa0175920 AT5G38120.1 42.647 544 288 9 31 562 12 543 5.44e-138 412
Msa0175920 AT1G20480.1 42.831 551 286 12 25 561 22 557 2.36e-135 405
Msa0175920 AT5G63380.1 43.038 553 285 12 25 561 12 550 7.78e-135 404
Msa0175920 AT1G20510.2 43.595 484 256 8 21 498 1 473 5.51e-123 371
Msa0175920 AT5G38120.3 41.165 515 279 9 31 533 12 514 1.66e-120 366
Msa0175920 AT1G20510.3 41.991 462 251 8 21 476 1 451 4.59e-110 337
Msa0175920 AT1G65060.1 41.309 489 276 5 81 561 71 556 1.66e-108 336
Msa0175920 AT5G38120.4 40.417 480 262 9 31 498 12 479 2.90e-107 330
Msa0175920 AT5G38120.2 40.417 480 262 9 31 498 12 479 2.90e-107 330
Msa0175920 AT3G21240.1 39.370 508 277 8 73 561 51 546 7.09e-107 332
Msa0175920 AT1G62940.1 40.927 496 280 6 74 561 42 532 1.59e-104 325
Msa0175920 AT3G21230.1 40.354 508 286 6 68 561 56 560 5.54e-104 325
Msa0175920 AT1G51680.1 39.759 498 276 7 82 561 62 553 9.07e-98 308
Msa0175920 AT3G21230.3 39.583 480 273 6 68 533 56 532 1.10e-91 292
Msa0175920 AT1G51680.3 38.655 476 268 7 76 533 56 525 1.74e-85 276
Msa0175920 AT3G21240.2 38.215 437 253 8 73 497 51 482 2.72e-80 261
Msa0175920 AT3G21240.3 38.215 437 253 8 73 497 51 482 2.72e-80 261
Msa0175920 AT1G20490.2 37.123 431 244 10 31 446 11 429 1.58e-79 258
Msa0175920 AT1G20490.1 37.123 431 244 10 31 446 11 429 1.58e-79 258
Msa0175920 AT3G21230.2 39.101 445 254 6 67 497 55 496 2.10e-79 259
Msa0175920 AT1G65060.2 38.670 406 238 5 81 478 71 473 6.24e-74 244
Msa0175920 AT1G51680.2 37.955 440 249 7 76 497 56 489 2.14e-72 240
Msa0175920 AT3G21230.4 34.853 373 226 6 68 426 56 425 3.50e-44 163
Msa0175920 AT3G48990.1 30.061 489 314 12 85 559 30 504 2.33e-41 157
Msa0175920 AT1G65880.1 27.920 548 323 17 85 572 32 567 1.40e-39 152
Msa0175920 AT1G30520.2 27.387 555 328 16 74 571 21 557 1.10e-38 150
Msa0175920 AT1G30520.1 27.387 555 328 16 74 571 21 557 1.10e-38 150
Msa0175920 AT1G21540.1 27.660 517 320 15 82 558 37 539 4.24e-38 148
Msa0175920 AT1G65890.1 27.170 530 320 17 85 561 32 548 2.85e-37 146
Msa0175920 AT1G20560.1 26.628 522 332 15 87 573 43 548 4.35e-35 139
Msa0175920 AT1G77240.1 25.919 517 329 16 80 558 34 534 3.10e-34 137
Msa0175920 AT1G21530.2 25.869 518 327 14 81 558 38 538 3.65e-34 137
Msa0175920 AT1G21530.1 26.104 521 320 15 81 558 38 536 8.72e-34 135
Msa0175920 AT5G16370.1 25.294 510 336 14 82 558 37 534 4.47e-33 133
Msa0175920 AT1G66120.1 27.203 522 323 17 85 561 32 541 2.06e-32 132
Msa0175920 AT1G75960.1 25.882 510 329 13 87 561 42 537 1.16e-30 126
Msa0175920 AT1G20560.2 26.525 377 253 9 210 573 105 470 1.51e-30 125
Msa0175920 AT5G16340.1 24.361 509 342 13 82 558 37 534 5.11e-30 124
Msa0175920 AT1G30520.3 27.115 461 274 14 74 493 21 460 7.32e-30 123
Msa0175920 AT1G30520.4 27.115 461 274 14 74 493 21 460 7.32e-30 123
Msa0175920 AT3G16910.1 28.158 380 237 14 210 566 193 559 2.20e-29 122
Msa0175920 AT3G16170.1 26.649 379 233 11 215 561 235 600 2.61e-27 117
Msa0175920 AT2G17650.1 25.290 518 322 17 87 556 88 588 1.68e-24 108
Msa0175920 AT1G76290.1 24.288 527 340 18 76 562 23 530 2.08e-24 107
Msa0175920 AT1G68270.1 25.094 530 301 18 85 561 32 518 5.69e-24 106
Msa0175920 AT5G27600.1 25.160 469 263 20 86 484 119 569 2.54e-20 95.5
Msa0175920 AT5G36880.2 23.660 541 351 18 79 569 191 719 1.77e-17 86.7
Msa0175920 AT1G49430.1 26.479 355 191 14 199 496 210 551 2.08e-17 86.3
Msa0175920 AT5G36880.6 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0175920 AT5G36880.3 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0175920 AT5G36880.5 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0175920 AT5G36880.1 23.660 541 351 18 79 569 141 669 2.10e-17 86.3
Msa0175920 AT2G47240.1 27.566 341 184 13 210 496 216 547 1.58e-16 83.6
Msa0175920 AT2G47240.2 27.566 341 184 13 210 496 216 547 1.58e-16 83.6
Msa0175920 AT2G47240.3 27.566 341 184 13 210 496 216 547 1.58e-16 83.6
Msa0175920 AT2G47240.4 27.566 341 184 13 210 496 157 488 1.61e-16 83.6
Msa0175920 AT1G77590.2 24.363 353 197 12 207 496 93 438 4.50e-16 81.6
Msa0175920 AT2G04350.2 24.576 354 185 15 207 491 268 608 4.22e-15 79.0
Msa0175920 AT2G04350.1 24.576 354 185 15 207 491 268 608 4.22e-15 79.0
Msa0175920 AT5G35930.4 23.495 515 335 16 87 556 59 559 5.25e-11 66.2

Find 146 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCTATGTTTCATGTCTA+TGG 0.192777 1_4:+62056192 Msa0175920:CDS
ACCCTTCTCACTTTCTTATA+TGG 0.212821 1_4:-62058463 None:intergenic
ATCAGCTTTCCACATGCTTC+TGG 0.219682 1_4:-62056558 None:intergenic
AAATAAAGCTATGGTGTAAT+TGG 0.225021 1_4:+62058710 Msa0175920:three_prime_UTR
GGACTGATGTTCTTTGGATT+TGG 0.232726 1_4:+62056213 Msa0175920:CDS
GAATGGTAAATTCCTGTTCT+TGG 0.248189 1_4:-62055496 None:intergenic
GTCTATGGACTGATGTTCTT+TGG 0.250992 1_4:+62056207 Msa0175920:CDS
GTTGAGTACAAGATTGAGTT+TGG 0.273727 1_4:-62055757 None:intergenic
TAACTGCTGCATCAACAATA+AGG 0.276654 1_4:-62057027 None:intergenic
GCCGCTATACTTTACTCTTC+AGG 0.280862 1_4:+62056051 Msa0175920:CDS
CCAGCAGAGCAGTTTCATTA+TGG 0.284701 1_4:-62056124 None:intergenic
ACCAATTTGTGTAACTCTTC+TGG 0.296211 1_4:-62055898 None:intergenic
GACTGATGTTCTTTGGATTT+GGG 0.298826 1_4:+62056214 Msa0175920:CDS
CTTTGTTGGGATTTCATGTT+TGG 0.301287 1_4:-62055536 None:intergenic
CTCTGTCTCCATTTGATAAC+AGG 0.330041 1_4:+62055461 Msa0175920:CDS
TTGTATAAGTATTGAGGTTA+AGG 0.339381 1_4:-62055234 None:intergenic
CATGTCATTCAATTTGTTGC+AGG 0.341335 1_4:+62057208 Msa0175920:CDS
CACCATAGCTTTATTTCTTT+AGG 0.341674 1_4:-62058703 None:intergenic
AATCAAGCACAAAGGGTATC+AGG 0.343261 1_4:+62056806 Msa0175920:CDS
CTACGTTTCACTCTCTTTGC+CGG 0.344130 1_4:+62055171 Msa0175920:five_prime_UTR
AAATGTCAGGAGAGGAGATT+TGG 0.351734 1_4:+62055410 Msa0175920:CDS
AAAGTGATAGACATTGAAAC+AGG 0.356914 1_4:+62056597 Msa0175920:CDS
CTACATAGTTGAACGAATAA+AGG 0.367598 1_4:+62056779 Msa0175920:CDS
AACTGCTGCATCAACAATAA+GGG 0.374049 1_4:-62057026 None:intergenic
CTTGCAACAGCTTTGTTCCA+TGG 0.383330 1_4:+62055694 Msa0175920:CDS
TCAGGTAGCTCCTGCAGAAC+TGG 0.384556 1_4:+62056982 Msa0175920:intron
ACCAGAAGAGTTACACAAAT+TGG 0.386896 1_4:+62055897 Msa0175920:CDS
TCAGTTGAAGAGTTAATAGA+AGG 0.392562 1_4:+62055976 Msa0175920:CDS
AGGGTTGAAGATGAAGAAAC+TGG 0.395025 1_4:+62057130 Msa0175920:intron
CACCTGAGCCAACCCTTCTT+AGG 0.395579 1_4:-62056398 None:intergenic
GCGAAGCAAGTACATGATTC+AGG 0.402603 1_4:+62055853 Msa0175920:CDS
CTTTACTCTTCAGGGACTAC+TGG 0.405377 1_4:+62056060 Msa0175920:CDS
TATTTCCTTGGGTTGAACTA+AGG 0.410515 1_4:+62056463 Msa0175920:CDS
GTTCAGAAAGCTATTTCCTT+GGG 0.412932 1_4:+62056452 Msa0175920:CDS
TGTTGAGATCTGGCTTTGTT+GGG 0.419272 1_4:-62055549 None:intergenic
TCAATTTGTCAGTAAAACTT+TGG 0.420873 1_4:+62058781 Msa0175920:three_prime_UTR
CCGCTATACTTTACTCTTCA+GGG 0.425254 1_4:+62056052 Msa0175920:CDS
GTTGAACTAAGGGCGGGTTA+TGG 0.435071 1_4:+62056474 Msa0175920:CDS
CCATAATGAAACTGCTCTGC+TGG 0.435438 1_4:+62056124 Msa0175920:CDS
GTTCCTACAATTCTCACCTC+TGG 0.439013 1_4:+62055931 Msa0175920:CDS
GTGTTGAGATCTGGCTTTGT+TGG 0.439500 1_4:-62055550 None:intergenic
AGCGGCTACGATCCAAGAAC+AGG 0.444190 1_4:+62055484 Msa0175920:CDS
CATTCAATTTGTTGCAGGCC+AGG 0.446682 1_4:+62057213 Msa0175920:CDS
GGATTTGGGTTGTTGTGTGT+TGG 0.451406 1_4:+62056228 Msa0175920:CDS
TTTCTAAGTTGTGAGTAACT+TGG 0.452000 1_4:+62058569 Msa0175920:exon
TTTCAAGCTTAACAAGAGAA+TGG 0.452313 1_4:-62055513 None:intergenic
TTCACCAATGAGTGGATAAC+TGG 0.452438 1_4:-62056342 None:intergenic
GCTATTATGAAAGAATATCT+AGG 0.453902 1_4:+62056669 Msa0175920:CDS
GTGATTTGTCTAGCCTAAGA+AGG 0.457731 1_4:+62056385 Msa0175920:CDS
AGCACAGATTCCAGTTCTGC+AGG 0.460066 1_4:-62056992 None:intergenic
TGGATAACTGGTGGCACTGC+TGG 0.462633 1_4:-62056330 None:intergenic
AGAGTTGCAGTGTTGAGATC+TGG 0.462977 1_4:-62055559 None:intergenic
CTCACCTCTGGGACATCTGA+TGG 0.463000 1_4:+62055943 Msa0175920:CDS
GTCCCAGAGGTGAGAATTGT+AGG 0.467301 1_4:-62055934 None:intergenic
CATGGACTTGAGATTAGAAA+AGG 0.469403 1_4:+62055712 Msa0175920:CDS
TTACACAAATTGGTCCCAAC+TGG 0.471916 1_4:+62055907 Msa0175920:CDS
GCATATGTGGTGAGGGCAGC+TGG 0.475126 1_4:+62057166 Msa0175920:CDS
CAACAATTGATTCAGAAGGT+TGG 0.475182 1_4:+62056712 Msa0175920:CDS
TGTCAGTTGTATAAGTATTG+AGG 0.478516 1_4:-62055240 None:intergenic
TTCTAAGTTGTGAGTAACTT+GGG 0.479667 1_4:+62058570 Msa0175920:exon
TAGCCGCTCCTGTTATCAAA+TGG 0.484875 1_4:-62055469 None:intergenic
TGGTTGAGGACAGGTGATCT+TGG 0.485306 1_4:+62056732 Msa0175920:CDS
GCAGTGTCTGATTGCTCCAC+AGG 0.486700 1_4:-62056030 None:intergenic
TGATTTAGTATTGAGAGGGT+TGG 0.488156 1_4:-62055824 None:intergenic
AGTTCAGAAAGCTATTTCCT+TGG 0.490058 1_4:+62056451 Msa0175920:CDS
AGGTGCTGCACCTTTGAGTA+AGG 0.492711 1_4:+62056416 Msa0175920:CDS
ATTTCCTTGGGTTGAACTAA+GGG 0.492805 1_4:+62056464 Msa0175920:CDS
CCTTCCTTGAGTGGAGGCAA+AGG 0.493651 1_4:-62056624 None:intergenic
CTTGGTTATATCGATGAGAA+TGG 0.496399 1_4:+62056750 Msa0175920:CDS
TTCACCAGATTCTTCTTCAA+CGG 0.499514 1_4:-62055437 None:intergenic
TAACTCTTCTGGTGCTGAGA+TGG 0.504393 1_4:-62055887 None:intergenic
ATGGACTAACAGAAAGTTGT+GGG 0.505501 1_4:+62056493 Msa0175920:CDS
GACATGAAACATAGGAATGA+AGG 0.505557 1_4:-62056187 None:intergenic
AGCCTAAGAAGGGTTGGCTC+AGG 0.509742 1_4:+62056396 Msa0175920:CDS
AGGAGCTAATCAAGCACAAA+GGG 0.511589 1_4:+62056799 Msa0175920:CDS
CATCAGCAAGATCTCCTTGT+GGG 0.511833 1_4:-62055597 None:intergenic
CACTCAAGGAAGGAGAGTTA+TGG 0.515549 1_4:+62056634 Msa0175920:CDS
TCATCCGTTGAAGAAGAATC+TGG 0.516179 1_4:+62055433 Msa0175920:CDS
GCCACCAGTTATCCACTCAT+TGG 0.516190 1_4:+62056338 Msa0175920:CDS
TGTTTAGCTGTGTTATCAGT+TGG 0.521525 1_4:+62055784 Msa0175920:CDS
GTGAAACATGCTAGCAAAGA+TGG 0.522574 1_4:+62056360 Msa0175920:CDS
TTGATTCAGAAGGTTGGTTG+AGG 0.524384 1_4:+62056718 Msa0175920:CDS
TCAGAAGGTTGGTTGAGGAC+AGG 0.530736 1_4:+62056723 Msa0175920:CDS
TTTCTGATTTAGTATTGAGA+GGG 0.531374 1_4:-62055828 None:intergenic
GAAGCCTTTGCCTCCACTCA+AGG 0.535824 1_4:+62056620 Msa0175920:CDS
TTCCTACAATTCTCACCTCT+GGG 0.539856 1_4:+62055932 Msa0175920:CDS
ACTAATCATAGTGTAACTTA+CGG 0.545558 1_4:+62055646 Msa0175920:CDS
CATGGAACAAAGCTGTTGCA+AGG 0.549110 1_4:-62055693 None:intergenic
CCTTGGGTTGAACTAAGGGC+GGG 0.553742 1_4:+62056468 Msa0175920:CDS
TCCTTGGGTTGAACTAAGGG+CGG 0.554693 1_4:+62056467 Msa0175920:CDS
AGAATTGTAGGAACTCCAGT+TGG 0.555282 1_4:-62055922 None:intergenic
AGTATACCGAGGTCAGCTGC+TGG 0.555861 1_4:+62058499 Msa0175920:CDS
CATAACTCTCCTTCCTTGAG+TGG 0.558671 1_4:-62056633 None:intergenic
TTTGAGTAAGGAAATGTCAC+TGG 0.559342 1_4:+62056428 Msa0175920:CDS
TGGTTTAGTATAAACTGAGT+TGG 0.560230 1_4:+62058801 Msa0175920:three_prime_UTR
ACCAATGGCATATGTGGTGA+GGG 0.561041 1_4:+62057159 Msa0175920:CDS
AGCCTAAAGAAATAAAGCTA+TGG 0.561824 1_4:+62058701 Msa0175920:three_prime_UTR
GCCCTCACCACATATGCCAT+TGG 0.565672 1_4:-62057160 None:intergenic
ATTTCTGATTTAGTATTGAG+AGG 0.569356 1_4:-62055829 None:intergenic
TTGTCTAGCCTAAGAAGGGT+TGG 0.570054 1_4:+62056390 Msa0175920:CDS
GAATTGTAGGAACTCCAGTT+GGG 0.571691 1_4:-62055921 None:intergenic
AGAATATCTAGGAAATATTG+AGG 0.571721 1_4:+62056680 Msa0175920:CDS
CTCACAACTTAGAAACAAGC+TGG 0.574328 1_4:-62058561 None:intergenic
AGAAGCCGCCTTATATCAGA+AGG 0.579500 1_4:+62058660 Msa0175920:three_prime_UTR
CCTTTGCCTCCACTCAAGGA+AGG 0.582843 1_4:+62056624 Msa0175920:CDS
AGTATTGAGAGGGTTGGCTG+TGG 0.583867 1_4:-62055818 None:intergenic
TGATTTGTCTAGCCTAAGAA+GGG 0.587869 1_4:+62056386 Msa0175920:CDS
AGTCCATAGACATGAAACAT+AGG 0.591137 1_4:-62056195 None:intergenic
ATCTTGCCAGCAGCTGACCT+CGG 0.591787 1_4:-62058505 None:intergenic
TCCATATAAGAAAGTGAGAA+GGG 0.594862 1_4:+62058462 Msa0175920:CDS
CTCCATATAAGAAAGTGAGA+AGG 0.595321 1_4:+62058461 Msa0175920:CDS
TACCAATGGCATATGTGGTG+AGG 0.595660 1_4:+62057158 Msa0175920:CDS
ACTGCAACAATTGATTCAGA+AGG 0.598082 1_4:+62056708 Msa0175920:CDS
ACTGCTGCATCAACAATAAG+GGG 0.600356 1_4:-62057025 None:intergenic
AGTGACATTTCCTTACTCAA+AGG 0.602475 1_4:-62056426 None:intergenic
AACTCTCCTTCCTTGAGTGG+AGG 0.608300 1_4:-62056630 None:intergenic
GCAGATACCAATGGCATATG+TGG 0.613364 1_4:+62057153 Msa0175920:CDS
AAGGAGCTAATCAAGCACAA+AGG 0.617428 1_4:+62056798 Msa0175920:CDS
TTACCCCTTCTGATATAAGG+CGG 0.622594 1_4:-62058665 None:intergenic
ATGGAACAAAGCTGTTGCAA+GGG 0.623142 1_4:-62055692 None:intergenic
GATTGCTCCACAGGAACATG+TGG 0.623243 1_4:-62056021 None:intergenic
CCCGCCCTTAGTTCAACCCA+AGG 0.635164 1_4:-62056468 None:intergenic
GGGACTACTGGAGTAAGCAA+AGG 0.641617 1_4:+62056072 Msa0175920:CDS
GTGCTATCACAGTTCCCACA+AGG 0.647014 1_4:+62055583 Msa0175920:CDS
CCCTGAAGAGTAAAGTATAG+CGG 0.649066 1_4:-62056052 None:intergenic
ACCAATGAGTGGATAACTGG+TGG 0.649415 1_4:-62056339 None:intergenic
GGGTTGAAGATGAAGAAACT+GGG 0.650548 1_4:+62057131 Msa0175920:intron
AGAATTGCCACATGTTCCTG+TGG 0.656792 1_4:+62056014 Msa0175920:CDS
AAAGCTCATCCAGAAGCATG+TGG 0.657133 1_4:+62056549 Msa0175920:CDS
AAGCCGCCTTATATCAGAAG+GGG 0.658392 1_4:+62058662 Msa0175920:three_prime_UTR
AGAAACTGGGCAGATACCAA+TGG 0.659161 1_4:+62057144 Msa0175920:CDS
TATGGACTAACAGAAAGTTG+TGG 0.659761 1_4:+62056492 Msa0175920:CDS
CTGCAACAAATTGAATGACA+TGG 0.661163 1_4:-62057207 None:intergenic
TAGCATGTTTCACCAATGAG+TGG 0.661752 1_4:-62056350 None:intergenic
TCTCCATTTGATAACAGGAG+CGG 0.663420 1_4:+62055466 Msa0175920:CDS
GAAACATAGGAATGAAGGCG+AGG 0.665139 1_4:-62056182 None:intergenic
CTTCTCGATAGCGACAAGCA+TGG 0.668123 1_4:-62056289 None:intergenic
GAAGCCGCCTTATATCAGAA+GGG 0.670001 1_4:+62058661 Msa0175920:three_prime_UTR
GCATCAGCAAGATCTCCTTG+TGG 0.671856 1_4:-62055598 None:intergenic
GGACTAACAGAAAGTTGTGG+GGG 0.674978 1_4:+62056495 Msa0175920:CDS
TGGACTAACAGAAAGTTGTG+GGG 0.694783 1_4:+62056494 Msa0175920:CDS
ATGAAGTGGAGTTCAAAGCG+AGG 0.696930 1_4:+62058637 Msa0175920:three_prime_UTR
GGAGAGTTATGGTTGAAAAG+CGG 0.697534 1_4:+62056645 Msa0175920:CDS
AGTAACTTGGGCATAAACGA+CGG 0.698899 1_4:+62058582 Msa0175920:exon
AAGTGATAGACATTGAAACA+GGG 0.705961 1_4:+62056598 Msa0175920:CDS
CTTTCCATCAGATGTCCCAG+AGG 0.738092 1_4:-62055947 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTAACTTAAATAATC+AGG - chr1_4:62057356-62057375 None:intergenic 10.0%
!!! TTTTTTTACGTATTATATAA+GGG - chr1_4:62057499-62057518 None:intergenic 10.0%
!!! TTTTTTTTACGTATTATATA+AGG - chr1_4:62057500-62057519 None:intergenic 10.0%
!! AGTAAATTACTTTAATTCTA+AGG + chr1_4:62056900-62056919 Msa0175920:intron 15.0%
!!! AGTTTTTATTAATACATATG+TGG + chr1_4:62056949-62056968 Msa0175920:intron 15.0%
!!! TTTGTATTTTAAACCTAATT+AGG - chr1_4:62057399-62057418 None:intergenic 15.0%
!!! TTTTTTACGTATTATATAAG+GGG - chr1_4:62057498-62057517 None:intergenic 15.0%
!!! ACTACCAAATAATTTTTTTT+AGG + chr1_4:62057585-62057604 Msa0175920:intron 15.0%
!! CTCACTAATATTAAAAATAT+CGG - chr1_4:62057921-62057940 None:intergenic 15.0%
!! TATGCCAAAAAAATTATTAT+GGG - chr1_4:62057990-62058009 None:intergenic 15.0%
!! ATATGCCAAAAAAATTATTA+TGG - chr1_4:62057991-62058010 None:intergenic 15.0%
!! TAAAAAAATAATAATGCAGA+GGG - chr1_4:62058215-62058234 None:intergenic 15.0%
!!! TCTGCATTATTATTTTTTTA+GGG + chr1_4:62058215-62058234 Msa0175920:intron 15.0%
!!! AAATAAATTTGAGAATTTTC+AGG + chr1_4:62058435-62058454 Msa0175920:intron 15.0%
!!! GATATTTTTCGTAAAATACT+AGG - chr1_4:62055283-62055302 None:intergenic 20.0%
!! ATTGAAACTGAAACTATTTA+TGG - chr1_4:62056841-62056860 None:intergenic 20.0%
!! ATAAATAGTTTCAGTTTCAA+TGG + chr1_4:62056840-62056859 Msa0175920:intron 20.0%
!! ATGAAAATAAGAGTGAAAAT+AGG - chr1_4:62057072-62057091 None:intergenic 20.0%
!! GTTGCCTAAAAAAAATTATT+TGG - chr1_4:62057592-62057611 None:intergenic 20.0%
!!! TACGGTATTTTATTTTGTTT+TGG - chr1_4:62057646-62057665 None:intergenic 20.0%
!! TGTTTATTATCCAACAAATT+GGG - chr1_4:62057895-62057914 None:intergenic 20.0%
!! ATGTTTATTATCCAACAAAT+TGG - chr1_4:62057896-62057915 None:intergenic 20.0%
!! AAAATTATTATGGGAGAAAT+TGG - chr1_4:62057981-62058000 None:intergenic 20.0%
!!! TTCTCCCATAATAATTTTTT+TGG + chr1_4:62057983-62058002 Msa0175920:intron 20.0%
!!! AGAAAAACCAAACTTTTTAT+AGG + chr1_4:62058016-62058035 Msa0175920:intron 20.0%
!!! GAAAAACCAAACTTTTTATA+GGG + chr1_4:62058017-62058036 Msa0175920:intron 20.0%
!! TATAGAAATCTTTGTGTTAT+AGG + chr1_4:62058048-62058067 Msa0175920:intron 20.0%
!!! AAAGAAATGTTTGGTTTATT+GGG - chr1_4:62058186-62058205 None:intergenic 20.0%
!!! TAAAGAAATGTTTGGTTTAT+TGG - chr1_4:62058187-62058206 None:intergenic 20.0%
!! AAAAAAATAATAATGCAGAG+GGG - chr1_4:62058214-62058233 None:intergenic 20.0%
!! CTAAAAAAATAATAATGCAG+AGG - chr1_4:62058216-62058235 None:intergenic 20.0%
!!! CTCTGCATTATTATTTTTTT+AGG + chr1_4:62058214-62058233 Msa0175920:intron 20.0%
!! AGAGAAATTAAAATGACTAT+AGG - chr1_4:62058397-62058416 None:intergenic 20.0%
!!! TTTTTCTTCAATTAATGAAG+TGG + chr1_4:62058623-62058642 Msa0175920:three_prime_UTR 20.0%
!! TTTTTGTCCTGAAATTTTTG+TGG - chr1_4:62055197-62055216 None:intergenic 25.0%
!!! TTGTATAAGTATTGAGGTTA+AGG - chr1_4:62055237-62055256 None:intergenic 25.0%
!! AACATATCTATCAATTTTGC+AGG + chr1_4:62055383-62055402 Msa0175920:intron 25.0%
!! ACATATCTATCAATTTTGCA+GGG + chr1_4:62055384-62055403 Msa0175920:intron 25.0%
! ACTAATCATAGTGTAACTTA+CGG + chr1_4:62055646-62055665 Msa0175920:CDS 25.0%
!! TTTCTGATTTAGTATTGAGA+GGG - chr1_4:62055831-62055850 None:intergenic 25.0%
!! ATTTCTGATTTAGTATTGAG+AGG - chr1_4:62055832-62055851 None:intergenic 25.0%
! GCTATTATGAAAGAATATCT+AGG + chr1_4:62056669-62056688 Msa0175920:CDS 25.0%
! AGAATATCTAGGAAATATTG+AGG + chr1_4:62056680-62056699 Msa0175920:CDS 25.0%
! AAAATAAGAGTGAAAATAGG+CGG - chr1_4:62057069-62057088 None:intergenic 25.0%
!!! TTATTTTACTGCATGAAATC+AGG - chr1_4:62057255-62057274 None:intergenic 25.0%
!! TTGTAATCATTGTTGCTTAT+TGG + chr1_4:62057285-62057304 Msa0175920:intron 25.0%
! ATAAAAACCATATGACATTC+AGG + chr1_4:62057425-62057444 Msa0175920:intron 25.0%
! TAAAAACCATATGACATTCA+GGG + chr1_4:62057426-62057445 Msa0175920:intron 25.0%
!!! AATTATTTGGTAGTGAAAAG+CGG - chr1_4:62057579-62057598 None:intergenic 25.0%
! ACTTTCTTGAATTGATTGAA+AGG - chr1_4:62057756-62057775 None:intergenic 25.0%
! AGGATTAAGATTAATTTCGT+AGG + chr1_4:62057825-62057844 Msa0175920:intron 25.0%
! TGAAAATTATCCCAATTTGT+TGG + chr1_4:62057882-62057901 Msa0175920:intron 25.0%
! TATTAGTGAGACACATTATT+AGG + chr1_4:62057931-62057950 Msa0175920:intron 25.0%
!!! GAAATTGGTGATATCATTTT+GGG - chr1_4:62057966-62057985 None:intergenic 25.0%
!!! AGAAATTGGTGATATCATTT+TGG - chr1_4:62057967-62057986 None:intergenic 25.0%
!! CTCAAGTATGTTAATGTTTT+AGG - chr1_4:62058105-62058124 None:intergenic 25.0%
! CATCCATCTTGTAAATATTT+CGG + chr1_4:62058131-62058150 Msa0175920:intron 25.0%
! ATCCATCTTGTAAATATTTC+GGG + chr1_4:62058132-62058151 Msa0175920:intron 25.0%
!! AAGAAATGTTTGGTTTATTG+GGG - chr1_4:62058185-62058204 None:intergenic 25.0%
!!! TAAATTTGAGAATTTTCAGG+TGG + chr1_4:62058438-62058457 Msa0175920:intron 25.0%
! AAATAAAGCTATGGTGTAAT+TGG + chr1_4:62058710-62058729 Msa0175920:three_prime_UTR 25.0%
! TCAATTTGTCAGTAAAACTT+TGG + chr1_4:62058781-62058800 Msa0175920:three_prime_UTR 25.0%
! TGTCAGTTGTATAAGTATTG+AGG - chr1_4:62055243-62055262 None:intergenic 30.0%
CAGCATCTATAGTATATACA+AGG - chr1_4:62055305-62055324 None:intergenic 30.0%
TTTCAAGCTTAACAAGAGAA+TGG - chr1_4:62055516-62055535 None:intergenic 30.0%
TCAGTTGAAGAGTTAATAGA+AGG + chr1_4:62055976-62055995 Msa0175920:CDS 30.0%
ATTCCTATGTTTCATGTCTA+TGG + chr1_4:62056192-62056211 Msa0175920:CDS 30.0%
!! ATCACTTTTGCACAAAAAGT+TGG - chr1_4:62056585-62056604 None:intergenic 30.0%
AAAGTGATAGACATTGAAAC+AGG + chr1_4:62056597-62056616 Msa0175920:CDS 30.0%
AAGTGATAGACATTGAAACA+GGG + chr1_4:62056598-62056617 Msa0175920:CDS 30.0%
CTACATAGTTGAACGAATAA+AGG + chr1_4:62056779-62056798 Msa0175920:CDS 30.0%
! CTAAGGTATCAAATTGAGTT+TGG + chr1_4:62056917-62056936 Msa0175920:intron 30.0%
GTATCAAATTGAGTTTGGAA+CGG + chr1_4:62056922-62056941 Msa0175920:intron 30.0%
! AATCATTGTTGCTTATTGGT+TGG + chr1_4:62057289-62057308 Msa0175920:intron 30.0%
TATTCTGACTTCACCTAATT+AGG + chr1_4:62057383-62057402 Msa0175920:intron 30.0%
TTAGGCATAATACGAGAAAA+GGG + chr1_4:62057472-62057491 Msa0175920:intron 30.0%
!! ATTATTTGGTAGTGAAAAGC+GGG - chr1_4:62057578-62057597 None:intergenic 30.0%
GATGCTATTGTTCATTTCTA+CGG - chr1_4:62057664-62057683 None:intergenic 30.0%
!!! TTTTGGAACTTTAGAAAGAC+AGG + chr1_4:62057805-62057824 Msa0175920:intron 30.0%
GACACATTATTAGGAACAAA+TGG + chr1_4:62057940-62057959 Msa0175920:intron 30.0%
! AAACCAAACTTTTTATAGGG+AGG + chr1_4:62058020-62058039 Msa0175920:intron 30.0%
! AACCAAACTTTTTATAGGGA+GGG + chr1_4:62058021-62058040 Msa0175920:intron 30.0%
ATATAGCAAATCTCTCCAAA+AGG + chr1_4:62058078-62058097 Msa0175920:intron 30.0%
TATAGCAAATCTCTCCAAAA+GGG + chr1_4:62058079-62058098 Msa0175920:intron 30.0%
TATTTACAAGATGGATGACT+GGG - chr1_4:62058128-62058147 None:intergenic 30.0%
ATATTTACAAGATGGATGAC+TGG - chr1_4:62058129-62058148 None:intergenic 30.0%
AGTTATTGCATCTAGATTGT+TGG - chr1_4:62058314-62058333 None:intergenic 30.0%
TCCATATAAGAAAGTGAGAA+GGG + chr1_4:62058462-62058481 Msa0175920:CDS 30.0%
TTTCTAAGTTGTGAGTAACT+TGG + chr1_4:62058569-62058588 Msa0175920:exon 30.0%
TTCTAAGTTGTGAGTAACTT+GGG + chr1_4:62058570-62058589 Msa0175920:exon 30.0%
! ATTTTACCCCTTCTGATATA+AGG - chr1_4:62058671-62058690 None:intergenic 30.0%
AGCCTAAAGAAATAAAGCTA+TGG + chr1_4:62058701-62058720 Msa0175920:three_prime_UTR 30.0%
!! CACCATAGCTTTATTTCTTT+AGG - chr1_4:62058706-62058725 None:intergenic 30.0%
TGGTTTAGTATAAACTGAGT+TGG + chr1_4:62058801-62058820 Msa0175920:three_prime_UTR 30.0%
TTGTCAATGCTAATGTAGTG+TGG - chr1_4:62055356-62055375 None:intergenic 35.0%
! TTTTGCAGGGAAAAAATGTC+AGG + chr1_4:62055397-62055416 Msa0175920:intron 35.0%
TTCACCAGATTCTTCTTCAA+CGG - chr1_4:62055440-62055459 None:intergenic 35.0%
GAATGGTAAATTCCTGTTCT+TGG - chr1_4:62055499-62055518 None:intergenic 35.0%
CTTTGTTGGGATTTCATGTT+TGG - chr1_4:62055539-62055558 None:intergenic 35.0%
! CTTTTCTAATCTCAAGTCCA+TGG - chr1_4:62055714-62055733 None:intergenic 35.0%
CATGGACTTGAGATTAGAAA+AGG + chr1_4:62055712-62055731 Msa0175920:CDS 35.0%
GTTGAGTACAAGATTGAGTT+TGG - chr1_4:62055760-62055779 None:intergenic 35.0%
TGTTTAGCTGTGTTATCAGT+TGG + chr1_4:62055784-62055803 Msa0175920:CDS 35.0%
! TGATTTAGTATTGAGAGGGT+TGG - chr1_4:62055827-62055846 None:intergenic 35.0%
ACCAGAAGAGTTACACAAAT+TGG + chr1_4:62055897-62055916 Msa0175920:CDS 35.0%
ACCAATTTGTGTAACTCTTC+TGG - chr1_4:62055901-62055920 None:intergenic 35.0%
GACATGAAACATAGGAATGA+AGG - chr1_4:62056190-62056209 None:intergenic 35.0%
AGTCCATAGACATGAAACAT+AGG - chr1_4:62056198-62056217 None:intergenic 35.0%
GACTGATGTTCTTTGGATTT+GGG + chr1_4:62056214-62056233 Msa0175920:CDS 35.0%
! TGATTTGTCTAGCCTAAGAA+GGG + chr1_4:62056386-62056405 Msa0175920:CDS 35.0%
AGTGACATTTCCTTACTCAA+AGG - chr1_4:62056429-62056448 None:intergenic 35.0%
TTTGAGTAAGGAAATGTCAC+TGG + chr1_4:62056428-62056447 Msa0175920:CDS 35.0%
! AGTTCAGAAAGCTATTTCCT+TGG + chr1_4:62056451-62056470 Msa0175920:CDS 35.0%
! GTTCAGAAAGCTATTTCCTT+GGG + chr1_4:62056452-62056471 Msa0175920:CDS 35.0%
!! TATTTCCTTGGGTTGAACTA+AGG + chr1_4:62056463-62056482 Msa0175920:CDS 35.0%
!! ATTTCCTTGGGTTGAACTAA+GGG + chr1_4:62056464-62056483 Msa0175920:CDS 35.0%
! TATGGACTAACAGAAAGTTG+TGG + chr1_4:62056492-62056511 Msa0175920:CDS 35.0%
! ATGGACTAACAGAAAGTTGT+GGG + chr1_4:62056493-62056512 Msa0175920:CDS 35.0%
! ACTGCAACAATTGATTCAGA+AGG + chr1_4:62056708-62056727 Msa0175920:CDS 35.0%
! CAACAATTGATTCAGAAGGT+TGG + chr1_4:62056712-62056731 Msa0175920:CDS 35.0%
CTTGGTTATATCGATGAGAA+TGG + chr1_4:62056750-62056769 Msa0175920:CDS 35.0%
AACTGCTGCATCAACAATAA+GGG - chr1_4:62057029-62057048 None:intergenic 35.0%
TAACTGCTGCATCAACAATA+AGG - chr1_4:62057030-62057049 None:intergenic 35.0%
ATGTGGACAAAAAATGTTGC+AGG + chr1_4:62057110-62057129 Msa0175920:intron 35.0%
TGTGGACAAAAAATGTTGCA+GGG + chr1_4:62057111-62057130 Msa0175920:intron 35.0%
CTGCAACAAATTGAATGACA+TGG - chr1_4:62057210-62057229 None:intergenic 35.0%
!! CATGTCATTCAATTTGTTGC+AGG + chr1_4:62057208-62057227 Msa0175920:CDS 35.0%
! CTTTTCTCGTATTATGCCTA+AGG - chr1_4:62057473-62057492 None:intergenic 35.0%
CTTAGGCATAATACGAGAAA+AGG + chr1_4:62057471-62057490 Msa0175920:intron 35.0%
ATCAAGAATGTGTCTCACAT+CGG + chr1_4:62057693-62057712 Msa0175920:intron 35.0%
!! GTTAATGTTTTAGGCCCTTT+TGG - chr1_4:62058096-62058115 None:intergenic 35.0%
GACCCGAAATATTTACAAGA+TGG - chr1_4:62058137-62058156 None:intergenic 35.0%
GGGGTGTATAAAGAAATGTT+TGG - chr1_4:62058195-62058214 None:intergenic 35.0%
TTGTTGGATGAATGTCATCA+GGG - chr1_4:62058298-62058317 None:intergenic 35.0%
ATTGTTGGATGAATGTCATC+AGG - chr1_4:62058299-62058318 None:intergenic 35.0%
CTCCATATAAGAAAGTGAGA+AGG + chr1_4:62058461-62058480 Msa0175920:CDS 35.0%
ACCCTTCTCACTTTCTTATA+TGG - chr1_4:62058466-62058485 None:intergenic 35.0%
!! GTTTTATTGACAGTATACCG+AGG + chr1_4:62058488-62058507 Msa0175920:CDS 35.0%
! TGTCCTGAAATTTTTGTGGC+CGG - chr1_4:62055193-62055212 None:intergenic 40.0%
AAATGTCAGGAGAGGAGATT+TGG + chr1_4:62055410-62055429 Msa0175920:CDS 40.0%
TCATCCGTTGAAGAAGAATC+TGG + chr1_4:62055433-62055452 Msa0175920:CDS 40.0%
! CTCTGTCTCCATTTGATAAC+AGG + chr1_4:62055461-62055480 Msa0175920:CDS 40.0%
! TCTCCATTTGATAACAGGAG+CGG + chr1_4:62055466-62055485 Msa0175920:CDS 40.0%
!! TGTTGAGATCTGGCTTTGTT+GGG - chr1_4:62055552-62055571 None:intergenic 40.0%
ATGGAACAAAGCTGTTGCAA+GGG - chr1_4:62055695-62055714 None:intergenic 40.0%
TTACACAAATTGGTCCCAAC+TGG + chr1_4:62055907-62055926 Msa0175920:CDS 40.0%
GAATTGTAGGAACTCCAGTT+GGG - chr1_4:62055924-62055943 None:intergenic 40.0%
AGAATTGTAGGAACTCCAGT+TGG - chr1_4:62055925-62055944 None:intergenic 40.0%
TTCCTACAATTCTCACCTCT+GGG + chr1_4:62055932-62055951 Msa0175920:CDS 40.0%
CCCTGAAGAGTAAAGTATAG+CGG - chr1_4:62056055-62056074 None:intergenic 40.0%
CCGCTATACTTTACTCTTCA+GGG + chr1_4:62056052-62056071 Msa0175920:CDS 40.0%
! GTCTATGGACTGATGTTCTT+TGG + chr1_4:62056207-62056226 Msa0175920:CDS 40.0%
GGACTGATGTTCTTTGGATT+TGG + chr1_4:62056213-62056232 Msa0175920:CDS 40.0%
TTCACCAATGAGTGGATAAC+TGG - chr1_4:62056345-62056364 None:intergenic 40.0%
TAGCATGTTTCACCAATGAG+TGG - chr1_4:62056353-62056372 None:intergenic 40.0%
GTGAAACATGCTAGCAAAGA+TGG + chr1_4:62056360-62056379 Msa0175920:CDS 40.0%
! GTGATTTGTCTAGCCTAAGA+AGG + chr1_4:62056385-62056404 Msa0175920:CDS 40.0%
TGGACTAACAGAAAGTTGTG+GGG + chr1_4:62056494-62056513 Msa0175920:CDS 40.0%
GGAGAGTTATGGTTGAAAAG+CGG + chr1_4:62056645-62056664 Msa0175920:CDS 40.0%
! TTGATTCAGAAGGTTGGTTG+AGG + chr1_4:62056718-62056737 Msa0175920:CDS 40.0%
AAGGAGCTAATCAAGCACAA+AGG + chr1_4:62056798-62056817 Msa0175920:CDS 40.0%
AGGAGCTAATCAAGCACAAA+GGG + chr1_4:62056799-62056818 Msa0175920:CDS 40.0%
!! AATCAAGCACAAAGGGTATC+AGG + chr1_4:62056806-62056825 Msa0175920:CDS 40.0%
! ATATGTGGATTGCTTGTGTC+AGG + chr1_4:62056964-62056983 Msa0175920:intron 40.0%
ACTGCTGCATCAACAATAAG+GGG - chr1_4:62057028-62057047 None:intergenic 40.0%
TAAGAGTGAAAATAGGCGGA+GGG - chr1_4:62057065-62057084 None:intergenic 40.0%
ATAAGAGTGAAAATAGGCGG+AGG - chr1_4:62057066-62057085 None:intergenic 40.0%
CTCTCTGTGACTTTCTTATG+TGG + chr1_4:62057093-62057112 Msa0175920:intron 40.0%
AGGGTTGAAGATGAAGAAAC+TGG + chr1_4:62057130-62057149 Msa0175920:intron 40.0%
GGGTTGAAGATGAAGAAACT+GGG + chr1_4:62057131-62057150 Msa0175920:intron 40.0%
CACCCTCCCTATAAAAAGTT+TGG - chr1_4:62058026-62058045 None:intergenic 40.0%
CTATAGGAAAACGAGAAAGC+AGG - chr1_4:62058381-62058400 None:intergenic 40.0%
CTCACAACTTAGAAACAAGC+TGG - chr1_4:62058564-62058583 None:intergenic 40.0%
AGTAACTTGGGCATAAACGA+CGG + chr1_4:62058582-62058601 Msa0175920:exon 40.0%
TTACCCCTTCTGATATAAGG+CGG - chr1_4:62058668-62058687 None:intergenic 40.0%
CTACGTTTCACTCTCTTTGC+CGG + chr1_4:62055171-62055190 Msa0175920:five_prime_UTR 45.0%
TTGCCGGCCACAAAAATTTC+AGG + chr1_4:62055187-62055206 Msa0175920:five_prime_UTR 45.0%
ATGCTAATGTAGTGTGGCTG+TGG - chr1_4:62055350-62055369 None:intergenic 45.0%
CAGGGAAAAAATGTCAGGAG+AGG + chr1_4:62055402-62055421 Msa0175920:intron 45.0%
TAGCCGCTCCTGTTATCAAA+TGG - chr1_4:62055472-62055491 None:intergenic 45.0%
!! GTGTTGAGATCTGGCTTTGT+TGG - chr1_4:62055553-62055572 None:intergenic 45.0%
! AGAGTTGCAGTGTTGAGATC+TGG - chr1_4:62055562-62055581 None:intergenic 45.0%
CATCAGCAAGATCTCCTTGT+GGG - chr1_4:62055600-62055619 None:intergenic 45.0%
CATGGAACAAAGCTGTTGCA+AGG - chr1_4:62055696-62055715 None:intergenic 45.0%
!! CTTGCAACAGCTTTGTTCCA+TGG + chr1_4:62055694-62055713 Msa0175920:CDS 45.0%
GCGAAGCAAGTACATGATTC+AGG + chr1_4:62055853-62055872 Msa0175920:CDS 45.0%
! TAACTCTTCTGGTGCTGAGA+TGG - chr1_4:62055890-62055909 None:intergenic 45.0%
GTTCCTACAATTCTCACCTC+TGG + chr1_4:62055931-62055950 Msa0175920:CDS 45.0%
AGAATTGCCACATGTTCCTG+TGG + chr1_4:62056014-62056033 Msa0175920:CDS 45.0%
GCCGCTATACTTTACTCTTC+AGG + chr1_4:62056051-62056070 Msa0175920:CDS 45.0%
CTTTACTCTTCAGGGACTAC+TGG + chr1_4:62056060-62056079 Msa0175920:CDS 45.0%
CCAGCAGAGCAGTTTCATTA+TGG - chr1_4:62056127-62056146 None:intergenic 45.0%
CCATAATGAAACTGCTCTGC+TGG + chr1_4:62056124-62056143 Msa0175920:CDS 45.0%
GAAACATAGGAATGAAGGCG+AGG - chr1_4:62056185-62056204 None:intergenic 45.0%
!! GGATTTGGGTTGTTGTGTGT+TGG + chr1_4:62056228-62056247 Msa0175920:CDS 45.0%
ACCAATGAGTGGATAACTGG+TGG - chr1_4:62056342-62056361 None:intergenic 45.0%
TTGTCTAGCCTAAGAAGGGT+TGG + chr1_4:62056390-62056409 Msa0175920:CDS 45.0%
GGACTAACAGAAAGTTGTGG+GGG + chr1_4:62056495-62056514 Msa0175920:CDS 45.0%
! AAAGCTCATCCAGAAGCATG+TGG + chr1_4:62056549-62056568 Msa0175920:CDS 45.0%
ATCAGCTTTCCACATGCTTC+TGG - chr1_4:62056561-62056580 None:intergenic 45.0%
CATAACTCTCCTTCCTTGAG+TGG - chr1_4:62056636-62056655 None:intergenic 45.0%
CACTCAAGGAAGGAGAGTTA+TGG + chr1_4:62056634-62056653 Msa0175920:CDS 45.0%
AAGAGTGAAAATAGGCGGAG+GGG - chr1_4:62057064-62057083 None:intergenic 45.0%
AGAAACTGGGCAGATACCAA+TGG + chr1_4:62057144-62057163 Msa0175920:CDS 45.0%
GCAGATACCAATGGCATATG+TGG + chr1_4:62057153-62057172 Msa0175920:CDS 45.0%
TACCAATGGCATATGTGGTG+AGG + chr1_4:62057158-62057177 Msa0175920:CDS 45.0%
ACCAATGGCATATGTGGTGA+GGG + chr1_4:62057159-62057178 Msa0175920:CDS 45.0%
!! CATTCAATTTGTTGCAGGCC+AGG + chr1_4:62057213-62057232 Msa0175920:CDS 45.0%
ATGAAATCAGGAGCCAAGAG+TGG - chr1_4:62057243-62057262 None:intergenic 45.0%
!!! TTTTTTTAGGGCCCCCCTAA+CGG + chr1_4:62058227-62058246 Msa0175920:intron 45.0%
GGAGCTAAGAGAAGCAGAAA+TGG - chr1_4:62058360-62058379 None:intergenic 45.0%
ATGAAGTGGAGTTCAAAGCG+AGG + chr1_4:62058637-62058656 Msa0175920:three_prime_UTR 45.0%
AGAAGCCGCCTTATATCAGA+AGG + chr1_4:62058660-62058679 Msa0175920:three_prime_UTR 45.0%
GAAGCCGCCTTATATCAGAA+GGG + chr1_4:62058661-62058680 Msa0175920:three_prime_UTR 45.0%
AAGCCGCCTTATATCAGAAG+GGG + chr1_4:62058662-62058681 Msa0175920:three_prime_UTR 45.0%
!!! ATGATATAATATATTTTTTT+TGG + chr1_4:62057788-62057807 Msa0175920:intron 5.0%
GTGCTATCACAGTTCCCACA+AGG + chr1_4:62055583-62055602 Msa0175920:CDS 50.0%
GCATCAGCAAGATCTCCTTG+TGG - chr1_4:62055601-62055620 None:intergenic 50.0%
!! AGTATTGAGAGGGTTGGCTG+TGG - chr1_4:62055821-62055840 None:intergenic 50.0%
GTCCCAGAGGTGAGAATTGT+AGG - chr1_4:62055937-62055956 None:intergenic 50.0%
CTTTCCATCAGATGTCCCAG+AGG - chr1_4:62055950-62055969 None:intergenic 50.0%
GATTGCTCCACAGGAACATG+TGG - chr1_4:62056024-62056043 None:intergenic 50.0%
GGGACTACTGGAGTAAGCAA+AGG + chr1_4:62056072-62056091 Msa0175920:CDS 50.0%
CTTCTCGATAGCGACAAGCA+TGG - chr1_4:62056292-62056311 None:intergenic 50.0%
GCCACCAGTTATCCACTCAT+TGG + chr1_4:62056338-62056357 Msa0175920:CDS 50.0%
! AGGTGCTGCACCTTTGAGTA+AGG + chr1_4:62056416-62056435 Msa0175920:CDS 50.0%
!! TCCTTGGGTTGAACTAAGGG+CGG + chr1_4:62056467-62056486 Msa0175920:CDS 50.0%
!! GTTGAACTAAGGGCGGGTTA+TGG + chr1_4:62056474-62056493 Msa0175920:CDS 50.0%
! TGTGGGGGAGCTACGTTTTT+CGG + chr1_4:62056510-62056529 Msa0175920:CDS 50.0%
AACTCTCCTTCCTTGAGTGG+AGG - chr1_4:62056633-62056652 None:intergenic 50.0%
! TCAGAAGGTTGGTTGAGGAC+AGG + chr1_4:62056723-62056742 Msa0175920:CDS 50.0%
TGGTTGAGGACAGGTGATCT+TGG + chr1_4:62056732-62056751 Msa0175920:CDS 50.0%
AGCACAGATTCCAGTTCTGC+AGG - chr1_4:62056995-62057014 None:intergenic 50.0%
!! CAATTTGTTGCAGGCCAGGT+AGG + chr1_4:62057217-62057236 Msa0175920:intron 50.0%
GAACGGCCCTGAATGTCATA+TGG - chr1_4:62057435-62057454 None:intergenic 50.0%
! GGGCCGTTCCTAGAGTTTTA+CGG + chr1_4:62057446-62057465 Msa0175920:intron 50.0%
! GGCCGTTCCTAGAGTTTTAC+GGG + chr1_4:62057447-62057466 Msa0175920:intron 50.0%
! GGCCCGTAAAACTCTAGGAA+CGG - chr1_4:62057452-62057471 None:intergenic 50.0%
CCTAAGGCCCGTAAAACTCT+AGG - chr1_4:62057457-62057476 None:intergenic 50.0%
! CCTAGAGTTTTACGGGCCTT+AGG + chr1_4:62057454-62057473 Msa0175920:intron 50.0%
TGGATGAATGTCATCAGGGC+CGG - chr1_4:62058294-62058313 None:intergenic 50.0%
AGCGGCTACGATCCAAGAAC+AGG + chr1_4:62055484-62055503 Msa0175920:CDS 55.0%
!! CTCACCTCTGGGACATCTGA+TGG + chr1_4:62055943-62055962 Msa0175920:CDS 55.0%
GCAGTGTCTGATTGCTCCAC+AGG - chr1_4:62056033-62056052 None:intergenic 55.0%
TGGATAACTGGTGGCACTGC+TGG - chr1_4:62056333-62056352 None:intergenic 55.0%
!! AGCCTAAGAAGGGTTGGCTC+AGG + chr1_4:62056396-62056415 Msa0175920:CDS 55.0%
CACCTGAGCCAACCCTTCTT+AGG - chr1_4:62056401-62056420 None:intergenic 55.0%
!! CCTTGGGTTGAACTAAGGGC+GGG + chr1_4:62056468-62056487 Msa0175920:CDS 55.0%
! GTGGGGGAGCTACGTTTTTC+GGG + chr1_4:62056511-62056530 Msa0175920:CDS 55.0%
!!! GGGAGCTACGTTTTTCGGGT+CGG + chr1_4:62056515-62056534 Msa0175920:CDS 55.0%
GAAGCCTTTGCCTCCACTCA+AGG + chr1_4:62056620-62056639 Msa0175920:CDS 55.0%
CCTTCCTTGAGTGGAGGCAA+AGG - chr1_4:62056627-62056646 None:intergenic 55.0%
CCTTTGCCTCCACTCAAGGA+AGG + chr1_4:62056624-62056643 Msa0175920:CDS 55.0%
TCAGGTAGCTCCTGCAGAAC+TGG + chr1_4:62056982-62057001 Msa0175920:intron 55.0%
AATAGGCGGAGGGGATCGTA+CGG - chr1_4:62057055-62057074 None:intergenic 55.0%
GCCCTCACCACATATGCCAT+TGG - chr1_4:62057163-62057182 None:intergenic 55.0%
AGTATACCGAGGTCAGCTGC+TGG + chr1_4:62058499-62058518 Msa0175920:CDS 55.0%
ATCTTGCCAGCAGCTGACCT+CGG - chr1_4:62058508-62058527 None:intergenic 55.0%
CCCGCCCTTAGTTCAACCCA+AGG - chr1_4:62056471-62056490 None:intergenic 60.0%
GCATATGTGGTGAGGGCAGC+TGG + chr1_4:62057166-62057185 Msa0175920:CDS 60.0%
CAGGCCAGGTAGGCCACTCT+TGG + chr1_4:62057227-62057246 Msa0175920:intron 65.0%
GGAGCCAAGAGTGGCCTACC+TGG - chr1_4:62057234-62057253 None:intergenic 65.0%
TTAGGGCCCCCCTAACGGCA+TGG + chr1_4:62058232-62058251 Msa0175920:intron 65.0%
TAGGGCCCCCCTAACGGCAT+GGG + chr1_4:62058233-62058252 Msa0175920:intron 65.0%
TAGGGCCCATGCCGTTAGGG+GGG - chr1_4:62058241-62058260 None:intergenic 65.0%
CTAGGGCCCATGCCGTTAGG+GGG - chr1_4:62058242-62058261 None:intergenic 65.0%
CCTAGGGCCCATGCCGTTAG+GGG - chr1_4:62058243-62058262 None:intergenic 65.0%
GCCTAGGGCCCATGCCGTTA+GGG - chr1_4:62058244-62058263 None:intergenic 65.0%
GCACTCCCGGCCTATGCCTA+GGG + chr1_4:62058268-62058287 Msa0175920:intron 65.0%
ATGTCATCAGGGCCGGCCCT+AGG - chr1_4:62058287-62058306 None:intergenic 65.0%
CCCCTAACGGCATGGGCCCT+AGG + chr1_4:62058240-62058259 Msa0175920:intron 70.0%
GGCCTAGGGCCCATGCCGTT+AGG - chr1_4:62058245-62058264 None:intergenic 70.0%
! GGCATAGGCCGGGAGTGCGA+CGG - chr1_4:62058266-62058285 None:intergenic 70.0%
CGCACTCCCGGCCTATGCCT+AGG + chr1_4:62058267-62058286 Msa0175920:intron 70.0%
TCCCGGCCTATGCCTAGGGC+CGG + chr1_4:62058272-62058291 Msa0175920:intron 70.0%
TCAGGGCCGGCCCTAGGCAT+AGG - chr1_4:62058281-62058300 None:intergenic 70.0%
GCCCTAGGCCGTCGCACTCC+CGG + chr1_4:62058255-62058274 Msa0175920:intron 75.0%
! GCCGGGAGTGCGACGGCCTA+GGG - chr1_4:62058259-62058278 None:intergenic 75.0%
GCCGGCCCTAGGCATAGGCC+GGG - chr1_4:62058276-62058295 None:intergenic 75.0%
GGCCGGCCCTAGGCATAGGC+CGG - chr1_4:62058277-62058296 None:intergenic 75.0%
!! GGCCGGGAGTGCGACGGCCT+AGG - chr1_4:62058260-62058279 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 62055152 62058833 62055152 ID=Msa0175920;Name=Msa0175920
chr1_4 mRNA 62055152 62058833 62055152 ID=Msa0175920-mRNA-1;Parent=Msa0175920;Name=Msa0175920-mRNA-1;_AED=0.07;_eAED=0.07;_QI=120|1|1|1|0.75|0.8|5|251|573
chr1_4 exon 62055152 62055273 62055152 ID=Msa0175920-mRNA-1:exon:19880;Parent=Msa0175920-mRNA-1
chr1_4 exon 62055405 62056827 62055405 ID=Msa0175920-mRNA-1:exon:19881;Parent=Msa0175920-mRNA-1
chr1_4 exon 62056986 62057053 62056986 ID=Msa0175920-mRNA-1:exon:19882;Parent=Msa0175920-mRNA-1
chr1_4 exon 62057132 62057234 62057132 ID=Msa0175920-mRNA-1:exon:19883;Parent=Msa0175920-mRNA-1
chr1_4 exon 62058457 62058833 62058457 ID=Msa0175920-mRNA-1:exon:19884;Parent=Msa0175920-mRNA-1
chr1_4 five_prime_UTR 62055152 62055271 62055152 ID=Msa0175920-mRNA-1:five_prime_utr;Parent=Msa0175920-mRNA-1
chr1_4 CDS 62055272 62055273 62055272 ID=Msa0175920-mRNA-1:cds;Parent=Msa0175920-mRNA-1
chr1_4 CDS 62055405 62056827 62055405 ID=Msa0175920-mRNA-1:cds;Parent=Msa0175920-mRNA-1
chr1_4 CDS 62056986 62057053 62056986 ID=Msa0175920-mRNA-1:cds;Parent=Msa0175920-mRNA-1
chr1_4 CDS 62057132 62057234 62057132 ID=Msa0175920-mRNA-1:cds;Parent=Msa0175920-mRNA-1
chr1_4 CDS 62058457 62058582 62058457 ID=Msa0175920-mRNA-1:cds;Parent=Msa0175920-mRNA-1
chr1_4 three_prime_UTR 62058583 62058833 62058583 ID=Msa0175920-mRNA-1:three_prime_utr;Parent=Msa0175920-mRNA-1
Gene Sequence

>Msa0175920

ATGGAAAAAATGTCAGGAGAGGAGATTTGGTCATCCGTTGAAGAAGAATCTGGTGAATCTCTGTCTCCATTTGATAACAGGAGCGGCTACGATCCAAGAACAGGAATTTACCATTCTCTTGTTAAGCTTGAAACCAAACATGAAATCCCAACAAAGCCAGATCTCAACACTGCAACTCTTGTGCTATCACAGTTCCCACAAGGAGATCTTGCTGATGCAAGAATTGCATTCATTGACTTAAATACTAATCATAGTGTAACTTACGGTGAGATTCGTCGATCAGTTTACTCCCTTGCAACAGCTTTGTTCCATGGACTTGAGATTAGAAAAGGCGACGTCGTGTTTTTATTGTCTCCAAACTCAATCTTGTACTCAACTATATGTTTAGCTGTGTTATCAGTTGGTGCAATTCTTACCACAGCCAACCCTCTCAATACTAAATCAGAAATCGCGAAGCAAGTACATGATTCAGGCGCTAAACTAGCCATCTCAGCACCAGAAGAGTTACACAAATTGGTCCCAACTGGAGTTCCTACAATTCTCACCTCTGGGACATCTGATGGAAAGTTTTTATCAGTTGAAGAGTTAATAGAAGGCTGCTATGATTCGCAAGAATTGCCACATGTTCCTGTGGAGCAATCAGACACTGCCGCTATACTTTACTCTTCAGGGACTACTGGAGTAAGCAAAGGTGTTGTTTTGACACATGCAAATCTCATAACCATAATGAAACTGCTCTGCTGGTCTGCTGATGTATCTACAGCACAAGACGATGTTTTCCTCGCCTTCATTCCTATGTTTCATGTCTATGGACTGATGTTCTTTGGATTTGGGTTGTTGTGTGTTGGTGTTACGACAGTTTTGATGCAAAAATATGATTTTCAAGCCATGCTTGTCGCTATCGAGAAGCACAAAGTTAATAACATACCAGCAGTGCCACCAGTTATCCACTCATTGGTGAAACATGCTAGCAAAGATGGCTGTGATTTGTCTAGCCTAAGAAGGGTTGGCTCAGGTGCTGCACCTTTGAGTAAGGAAATGTCACTGGAGTTCAGAAAGCTATTTCCTTGGGTTGAACTAAGGGCGGGTTATGGACTAACAGAAAGTTGTGGGGGAGCTACGTTTTTCGGGTCGGATAAAGATGCTAAAGCTCATCCAGAAGCATGTGGAAAGCTGATTCCAACTTTTTGTGCAAAAGTGATAGACATTGAAACAGGGAAGCCTTTGCCTCCACTCAAGGAAGGAGAGTTATGGTTGAAAAGCGGTGCTATTATGAAAGAATATCTAGGAAATATTGAGGCAACAACTGCAACAATTGATTCAGAAGGTTGGTTGAGGACAGGTGATCTTGGTTATATCGATGAGAATGGAATTGTCTACATAGTTGAACGAATAAAGGAGCTAATCAAGCACAAAGGGTATCAGGTAGCTCCTGCAGAACTGGAATCTGTGCTGCTAAGTCATCCCCTTATTGTTGATGCAGCAGTTATTCCGGTTGAAGATGAAGAAACTGGGCAGATACCAATGGCATATGTGGTGAGGGCAGCTGGTTCTCAACTATCAGAAGACCATGTCATTCAATTTGTTGCAGGCCAGGTGGCTCCATATAAGAAAGTGAGAAGGGTGAGTTTTATTGACAGTATACCGAGGTCAGCTGCTGGCAAGATATTGCGAAAAGATCTTGTTTTGCAAAGCAAATACCAGCTTGTTTCTAAGTTGTGA
Protein sequence

>Msa0175920

MEKMSGEEIWSSVEEESGESLSPFDNRSGYDPRTGIYHSLVKLETKHEIPTKPDLNTATLVLSQFPQGDLADARIAFIDLNTNHSVTYGEIRRSVYSLATALFHGLEIRKGDVVFLLSPNSILYSTICLAVLSVGAILTTANPLNTKSEIAKQVHDSGAKLAISAPEELHKLVPTGVPTILTSGTSDGKFLSVEELIEGCYDSQELPHVPVEQSDTAAILYSSGTTGVSKGVVLTHANLITIMKLLCWSADVSTAQDDVFLAFIPMFHVYGLMFFGFGLLCVGVTTVLMQKYDFQAMLVAIEKHKVNNIPAVPPVIHSLVKHASKDGCDLSSLRRVGSGAAPLSKEMSLEFRKLFPWVELRAGYGLTESCGGATFFGSDKDAKAHPEACGKLIPTFCAKVIDIETGKPLPPLKEGELWLKSGAIMKEYLGNIEATTATIDSEGWLRTGDLGYIDENGIVYIVERIKELIKHKGYQVAPAELESVLLSHPLIVDAAVIPVEDEETGQIPMAYVVRAAGSQLSEDHVIQFVAGQVAPYKKVRRVSFIDSIPRSAAGKILRKDLVLQSKYQLVSKL*