AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1033970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1033970 MtrunA17_Chr7g0256851 86.087 115 2 1 1 101 1 115 7.72e-65 191
Msa1033970 MtrunA17_Chr3g0085831 44.231 104 42 3 1 88 1 104 1.47e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1033970 AT2G41430.5 52.500 80 20 2 1 62 1 80 3.61e-19 77.4
Msa1033970 AT2G41430.4 52.500 80 20 2 1 62 1 80 3.61e-19 77.4
Msa1033970 AT2G41430.2 52.500 80 20 2 1 62 1 80 3.61e-19 77.4
Msa1033970 AT2G41430.1 52.500 80 20 2 1 62 1 80 3.61e-19 77.4
Msa1033970 AT2G41430.3 52.500 80 20 2 1 62 1 80 4.89e-19 75.9

Find 34 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAGGTAAGAAAGTTTA+TGG 0.140300 7_1:+69366361 None:intergenic
TTTCTTACCTGTCTTCAAAA+TGG 0.243639 7_1:-69366353 Msa1033970:three_prime_UTR
ATTGGTCAATTGATTGCTTC+TGG 0.249156 7_1:+69366254 None:intergenic
TTCAAGATGGCATTAGTTTC+AGG 0.269984 7_1:-69367121 None:intergenic
TTCCTTCTTACCAAAATCTA+TGG 0.308472 7_1:-69366513 Msa1033970:three_prime_UTR
CTTTCTGTAACTTCTTGTAT+AGG 0.333956 7_1:-69366557 Msa1033970:three_prime_UTR
GGAATGTAAAGTGGTGCATT+AGG 0.338731 7_1:+69367079 None:intergenic
GACGGTCTTGCCTTAGCTTT+CGG 0.365977 7_1:-69366611 Msa1033970:three_prime_UTR
CAAGGAGCAAAGAAATTTAC+TGG 0.372256 7_1:-69366683 Msa1033970:CDS
AGTAAGTTTATTATTACAAT+TGG 0.386330 7_1:+69366236 None:intergenic
GTCACCAGCTGCCACCATTC+CGG 0.393504 7_1:+69367025 None:intergenic
ACCGTCGCATTCAGCGTCTA+TGG 0.413218 7_1:+69366628 None:intergenic
GAATGTAAAGTGGTGCATTA+GGG 0.425842 7_1:+69367080 None:intergenic
AGACAGGTAAGAAAGTTTAT+GGG 0.447864 7_1:+69366362 None:intergenic
TTCTCCGGAATGGTGGCAGC+TGG 0.463269 7_1:-69367029 Msa1033970:intron
GCTTCTGGACATGTATTGTA+TGG 0.492883 7_1:+69366269 None:intergenic
GCATCAAGATCAAACGCATC+AGG 0.498917 7_1:+69366896 None:intergenic
ATTTACTGGATGAGCTAAAG+AGG 0.519766 7_1:-69366669 Msa1033970:exon
TGTAACTTCTTGTATAGGCA+AGG 0.531546 7_1:-69366552 Msa1033970:three_prime_UTR
TGACAGAACTCAGAAGTGTA+TGG 0.537723 7_1:-69366304 Msa1033970:three_prime_UTR
TCGACATAACGAAATGCAGC+TGG 0.542944 7_1:+69367058 None:intergenic
GATGATGTAGATGAGTTTGA+CGG 0.549584 7_1:-69366941 Msa1033970:CDS
AGCTGCATTTCGTTATGTCG+AGG 0.550315 7_1:-69367056 Msa1033970:CDS
AGCCAGCAACATGATGATGA+AGG 0.556932 7_1:-69366971 Msa1033970:intron
AGCTAAATAACCGAAAGCTA+AGG 0.582950 7_1:+69366601 None:intergenic
ACCATAGACGCTGAATGCGA+CGG 0.583100 7_1:-69366629 Msa1033970:three_prime_UTR
AGAGTTTATCAAGTCTTCTG+AGG 0.595138 7_1:-69366840 Msa1033970:CDS
ATCAAGTCTTCTGAGGCACA+AGG 0.619098 7_1:-69366833 Msa1033970:intron
AAATCTATGGTAAAATAGAG+AGG 0.627618 7_1:-69366500 Msa1033970:three_prime_UTR
AAGATGGCATTAGTTTCAGG+AGG 0.635369 7_1:-69367118 Msa1033970:CDS
TGATGCGTTTGATCTTGATG+CGG 0.644823 7_1:-69366894 Msa1033970:CDS
TATAACAGCTCTTACAACAT+CGG 0.657333 7_1:+69366441 None:intergenic
AATGCAGCTGGAATGTAAAG+TGG 0.671808 7_1:+69367070 None:intergenic
ACAGAAAGTTAAAACTGACA+TGG 0.696360 7_1:+69366572 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTTCCTCTGAAAAATAA+CGG + chr7_1:69366642-69366661 None:intergenic 25.0%
!! CTCTATTTTACCATAGATTT+TGG + chr7_1:69366853-69366872 None:intergenic 25.0%
! AAATCTATGGTAAAATAGAG+AGG - chr7_1:69366853-69366872 Msa1033970:CDS 25.0%
!! AACTTTCCTCTGAAAAATAA+CGG + chr7_1:69366642-69366661 None:intergenic 25.0%
!! CTCTATTTTACCATAGATTT+TGG + chr7_1:69366853-69366872 None:intergenic 25.0%
! AAATCTATGGTAAAATAGAG+AGG - chr7_1:69366853-69366872 Msa1033970:CDS 25.0%
!!! AACATACCGTTATTTTTCAG+AGG - chr7_1:69366633-69366652 Msa1033970:three_prime_UTR 30.0%
!! TTTTTCAGAGGAAAGTTTTG+TGG - chr7_1:69366645-69366664 Msa1033970:three_prime_UTR 30.0%
ACAGAAAGTTAAAACTGACA+TGG + chr7_1:69366784-69366803 None:intergenic 30.0%
CTTTCTGTAACTTCTTGTAT+AGG - chr7_1:69366796-69366815 Msa1033970:intron 30.0%
TTCCTTCTTACCAAAATCTA+TGG - chr7_1:69366840-69366859 Msa1033970:CDS 30.0%
!! TACCATAGATTTTGGTAAGA+AGG + chr7_1:69366845-69366864 None:intergenic 30.0%
TATAACAGCTCTTACAACAT+CGG + chr7_1:69366915-69366934 None:intergenic 30.0%
AGACAGGTAAGAAAGTTTAT+GGG + chr7_1:69366994-69367013 None:intergenic 30.0%
AAGACAGGTAAGAAAGTTTA+TGG + chr7_1:69366995-69367014 None:intergenic 30.0%
TTTCTTACCTGTCTTCAAAA+TGG - chr7_1:69367000-69367019 Msa1033970:intron 30.0%
!!! AACATACCGTTATTTTTCAG+AGG - chr7_1:69366633-69366652 Msa1033970:three_prime_UTR 30.0%
!! TTTTTCAGAGGAAAGTTTTG+TGG - chr7_1:69366645-69366664 Msa1033970:three_prime_UTR 30.0%
ACAGAAAGTTAAAACTGACA+TGG + chr7_1:69366784-69366803 None:intergenic 30.0%
CTTTCTGTAACTTCTTGTAT+AGG - chr7_1:69366796-69366815 Msa1033970:intron 30.0%
TTCCTTCTTACCAAAATCTA+TGG - chr7_1:69366840-69366859 Msa1033970:CDS 30.0%
!! TACCATAGATTTTGGTAAGA+AGG + chr7_1:69366845-69366864 None:intergenic 30.0%
TATAACAGCTCTTACAACAT+CGG + chr7_1:69366915-69366934 None:intergenic 30.0%
AGACAGGTAAGAAAGTTTAT+GGG + chr7_1:69366994-69367013 None:intergenic 30.0%
AAGACAGGTAAGAAAGTTTA+TGG + chr7_1:69366995-69367014 None:intergenic 30.0%
TTTCTTACCTGTCTTCAAAA+TGG - chr7_1:69367000-69367019 Msa1033970:intron 30.0%
GAATGTAAAGTGGTGCATTA+GGG + chr7_1:69366276-69366295 None:intergenic 35.0%
! TTATGTCGAGGATTTTTCTC+CGG - chr7_1:69366309-69366328 Msa1033970:three_prime_UTR 35.0%
GATGATGTAGATGAGTTTGA+CGG - chr7_1:69366412-69366431 Msa1033970:three_prime_UTR 35.0%
AGAGTTTATCAAGTCTTCTG+AGG - chr7_1:69366513-69366532 Msa1033970:three_prime_UTR 35.0%
CAAGGAGCAAAGAAATTTAC+TGG - chr7_1:69366670-69366689 Msa1033970:exon 35.0%
ATTTACTGGATGAGCTAAAG+AGG - chr7_1:69366684-69366703 Msa1033970:CDS 35.0%
AGCTAAATAACCGAAAGCTA+AGG + chr7_1:69366755-69366774 None:intergenic 35.0%
TGTAACTTCTTGTATAGGCA+AGG - chr7_1:69366801-69366820 Msa1033970:intron 35.0%
!! AATGCATCCATTTTGAAGAC+AGG + chr7_1:69367010-69367029 None:intergenic 35.0%
ATTGGTCAATTGATTGCTTC+TGG + chr7_1:69367102-69367121 None:intergenic 35.0%
GAATGTAAAGTGGTGCATTA+GGG + chr7_1:69366276-69366295 None:intergenic 35.0%
! TTATGTCGAGGATTTTTCTC+CGG - chr7_1:69366309-69366328 Msa1033970:three_prime_UTR 35.0%
GATGATGTAGATGAGTTTGA+CGG - chr7_1:69366412-69366431 Msa1033970:three_prime_UTR 35.0%
AGAGTTTATCAAGTCTTCTG+AGG - chr7_1:69366513-69366532 Msa1033970:three_prime_UTR 35.0%
CAAGGAGCAAAGAAATTTAC+TGG - chr7_1:69366670-69366689 Msa1033970:exon 35.0%
ATTTACTGGATGAGCTAAAG+AGG - chr7_1:69366684-69366703 Msa1033970:CDS 35.0%
AGCTAAATAACCGAAAGCTA+AGG + chr7_1:69366755-69366774 None:intergenic 35.0%
TGTAACTTCTTGTATAGGCA+AGG - chr7_1:69366801-69366820 Msa1033970:intron 35.0%
!! AATGCATCCATTTTGAAGAC+AGG + chr7_1:69367010-69367029 None:intergenic 35.0%
ATTGGTCAATTGATTGCTTC+TGG + chr7_1:69367102-69367121 None:intergenic 35.0%
GGAATGTAAAGTGGTGCATT+AGG + chr7_1:69366277-69366296 None:intergenic 40.0%
AATGCAGCTGGAATGTAAAG+TGG + chr7_1:69366286-69366305 None:intergenic 40.0%
TGATGCGTTTGATCTTGATG+CGG - chr7_1:69366459-69366478 Msa1033970:three_prime_UTR 40.0%
! TGACAGAACTCAGAAGTGTA+TGG - chr7_1:69367049-69367068 Msa1033970:CDS 40.0%
GCTTCTGGACATGTATTGTA+TGG + chr7_1:69367087-69367106 None:intergenic 40.0%
GGAATGTAAAGTGGTGCATT+AGG + chr7_1:69366277-69366296 None:intergenic 40.0%
AATGCAGCTGGAATGTAAAG+TGG + chr7_1:69366286-69366305 None:intergenic 40.0%
TGATGCGTTTGATCTTGATG+CGG - chr7_1:69366459-69366478 Msa1033970:three_prime_UTR 40.0%
! TGACAGAACTCAGAAGTGTA+TGG - chr7_1:69367049-69367068 Msa1033970:CDS 40.0%
GCTTCTGGACATGTATTGTA+TGG + chr7_1:69367087-69367106 None:intergenic 40.0%
TCGACATAACGAAATGCAGC+TGG + chr7_1:69366298-69366317 None:intergenic 45.0%
AGCTGCATTTCGTTATGTCG+AGG - chr7_1:69366297-69366316 Msa1033970:three_prime_UTR 45.0%
! TCGAGGATTTTTCTCCGGAA+TGG - chr7_1:69366314-69366333 Msa1033970:three_prime_UTR 45.0%
! AGGATTTTTCTCCGGAATGG+TGG - chr7_1:69366317-69366336 Msa1033970:three_prime_UTR 45.0%
AGCTGGTGACAACACTGAAA+TGG - chr7_1:69366341-69366360 Msa1033970:three_prime_UTR 45.0%
GCTGGCTAAGTCAGTAATCA+TGG + chr7_1:69366369-69366388 None:intergenic 45.0%
! AGCCAGCAACATGATGATGA+AGG - chr7_1:69366382-69366401 Msa1033970:three_prime_UTR 45.0%
AGCCTTCATCATCATGTTGC+TGG + chr7_1:69366387-69366406 None:intergenic 45.0%
GCATCAAGATCAAACGCATC+AGG + chr7_1:69366460-69366479 None:intergenic 45.0%
!! ATCAAGTCTTCTGAGGCACA+AGG - chr7_1:69366520-69366539 Msa1033970:three_prime_UTR 45.0%
!! GAGGAAAGTTTTGTGGAGCA+AGG - chr7_1:69366652-69366671 Msa1033970:three_prime_UTR 45.0%
TCGACATAACGAAATGCAGC+TGG + chr7_1:69366298-69366317 None:intergenic 45.0%
AGCTGCATTTCGTTATGTCG+AGG - chr7_1:69366297-69366316 Msa1033970:three_prime_UTR 45.0%
! TCGAGGATTTTTCTCCGGAA+TGG - chr7_1:69366314-69366333 Msa1033970:three_prime_UTR 45.0%
! AGGATTTTTCTCCGGAATGG+TGG - chr7_1:69366317-69366336 Msa1033970:three_prime_UTR 45.0%
AGCTGGTGACAACACTGAAA+TGG - chr7_1:69366341-69366360 Msa1033970:three_prime_UTR 45.0%
GCTGGCTAAGTCAGTAATCA+TGG + chr7_1:69366369-69366388 None:intergenic 45.0%
! AGCCAGCAACATGATGATGA+AGG - chr7_1:69366382-69366401 Msa1033970:three_prime_UTR 45.0%
AGCCTTCATCATCATGTTGC+TGG + chr7_1:69366387-69366406 None:intergenic 45.0%
GCATCAAGATCAAACGCATC+AGG + chr7_1:69366460-69366479 None:intergenic 45.0%
!! ATCAAGTCTTCTGAGGCACA+AGG - chr7_1:69366520-69366539 Msa1033970:three_prime_UTR 45.0%
!! GAGGAAAGTTTTGTGGAGCA+AGG - chr7_1:69366652-69366671 Msa1033970:three_prime_UTR 45.0%
ACCATAGACGCTGAATGCGA+CGG - chr7_1:69366724-69366743 Msa1033970:intron 50.0%
! GACGGTCTTGCCTTAGCTTT+CGG - chr7_1:69366742-69366761 Msa1033970:intron 50.0%
ACCATAGACGCTGAATGCGA+CGG - chr7_1:69366724-69366743 Msa1033970:intron 50.0%
! GACGGTCTTGCCTTAGCTTT+CGG - chr7_1:69366742-69366761 Msa1033970:intron 50.0%
ACCGTCGCATTCAGCGTCTA+TGG + chr7_1:69366728-69366747 None:intergenic 55.0%
ACCGTCGCATTCAGCGTCTA+TGG + chr7_1:69366728-69366747 None:intergenic 55.0%
TTCTCCGGAATGGTGGCAGC+TGG - chr7_1:69366324-69366343 Msa1033970:three_prime_UTR 60.0%
GTCACCAGCTGCCACCATTC+CGG + chr7_1:69366331-69366350 None:intergenic 60.0%
TTCTCCGGAATGGTGGCAGC+TGG - chr7_1:69366324-69366343 Msa1033970:three_prime_UTR 60.0%
GTCACCAGCTGCCACCATTC+CGG + chr7_1:69366331-69366350 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 69366238 69367137 69366238 ID=Msa1033970;Name=Msa1033970
chr7_1 mRNA 69366238 69367137 69366238 ID=Msa1033970-mRNA-1;Parent=Msa1033970;Name=Msa1033970-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.5|0.33|1|1|1|3|441|101
chr7_1 exon 69366238 69366722 69366238 ID=Msa1033970-mRNA-1:exon:16285;Parent=Msa1033970-mRNA-1
chr7_1 exon 69366834 69366987 69366834 ID=Msa1033970-mRNA-1:exon:16284;Parent=Msa1033970-mRNA-1
chr7_1 exon 69367030 69367137 69367030 ID=Msa1033970-mRNA-1:exon:16283;Parent=Msa1033970-mRNA-1
chr7_1 CDS 69367030 69367137 69367030 ID=Msa1033970-mRNA-1:cds;Parent=Msa1033970-mRNA-1
chr7_1 CDS 69366834 69366987 69366834 ID=Msa1033970-mRNA-1:cds;Parent=Msa1033970-mRNA-1
chr7_1 CDS 69366679 69366722 69366679 ID=Msa1033970-mRNA-1:cds;Parent=Msa1033970-mRNA-1
chr7_1 three_prime_UTR 69366238 69366678 69366238 ID=Msa1033970-mRNA-1:three_prime_utr;Parent=Msa1033970-mRNA-1
Gene Sequence

>Msa1033970

ATGGCATTAGTTTCAGGAGGAAGATCAACACTAAACCCTAATGCACCACTTTACATTCCAGCTGCATTTCGTTATGTCGAGGATTTTTCTCCGGAATGGTGGCAGCTGCAACATGATGATGAAGGCTTTTATGATGATGTAGATGAGTTTGACGGCAATGATGTCGTTGATTTGCTTCCTGATGCGTTTGATCTTGATGCGGATGAAGATCTTTCGATTGAAGCTCAGCTTGAAGAGTTTATCAAGTCTTCTGAGGCACAAGAGGAAAGTTTTGTGGAGCAAGGAGCAAAGAAATTTACTGGATGA

Protein sequence

>Msa1033970

MALVSGGRSTLNPNAPLYIPAAFRYVEDFSPEWWQLQHDDEGFYDDVDEFDGNDVVDLLPDAFDLDADEDLSIEAQLEEFIKSSEAQEESFVEQGAKKFTG*