AlfalfaGEDB | Msa1211050

M. sativa cultivar zhongmu-4 / Msa1211050

KEGG: K09008
GO: -
PFAM: PF04430.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211050	sp\|Q6DFN1\|NDUF3_XENTR	35.780	109	70	0	66	174	66	174	8.80e-21	87.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211050	B7FHB1	94.413	179	8	2	1	179	1	177	4.38e-118	341

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa1211050	0.825249	5.195662e-54	-8.615850e-47
Msa0019120	Msa1211050	-0.808268	3.366240e-50	-8.615850e-47
Msa0030280	Msa1211050	-0.801260	9.791269e-49	-8.615850e-47
Msa0036370	Msa1211050	-0.825270	5.135435e-54	-8.615850e-47
Msa0047280	Msa1211050	-0.802145	6.447271e-49	-8.615850e-47
Msa0072170	Msa1211050	-0.803934	2.750291e-49	-8.615850e-47
Msa0090030	Msa1211050	-0.806181	9.318648e-50	-8.615850e-47
Msa0094010	Msa1211050	-0.814273	1.675646e-51	-8.615850e-47
Msa0099510	Msa1211050	-0.826894	2.111789e-54	-8.615850e-47
Msa0104610	Msa1211050	-0.815163	1.063991e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211050	MtrunA17_Chr8g0337221	94.413	179	8	2	1	179	1	177	8.42e-122	341

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211050	AT2G44525.1	72.626	179	40	2	1	179	1	170	4.11e-92	266
Msa1211050	AT3G60150.1	71.508	179	42	2	1	179	1	170	1.84e-89	259
Msa1211050	AT3G60150.4	65.789	152	43	2	1	152	1	143	2.03e-68	205
Msa1211050	AT3G60150.2	67.619	105	25	2	1	105	1	96	9.84e-44	142
Msa1211050	AT3G60150.3	67.619	105	25	2	1	105	1	96	9.84e-44	142

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Find 70 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCAACCTGGAATAGGG+CGG	0.690412	8_1:-70415069	None:intergenic
AAATGAAGAAGGTCGCATTG+TGG	0.696515	8_1:+70416727	Msa1211050:CDS
AACAAGAAGTTCGTGGGTTT+GGG	0.250066	8_1:+70412969	Msa1211050:five_prime_UTR
AACTCCATATGGAAGAAGTG+CGG	0.679978	8_1:-70416753	None:intergenic
AATACTCTCTTCCAAATTAA+TGG	0.249280	8_1:-70417273	None:intergenic
ACAACACAACAAGAAGTTCG+TGG	0.601920	8_1:+70412962	Msa1211050:five_prime_UTR
ACAAGAAGTTCGTGGGTTTG+GGG	0.622996	8_1:+70412970	Msa1211050:five_prime_UTR
ACGGAGAGAGGGCAAGGAAT+TGG	0.326466	8_1:-70413128	None:intergenic
ACTTCTTGTTGTGTTGTTTC+TGG	0.118738	8_1:-70412956	None:intergenic
AGACGGTTTATTCGGTCTAC+TGG	0.478171	8_1:+70415631	Msa1211050:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATTCAGAGAAACAAAAAGA+TGG	+	chr8_1:70413003-70413022	Msa1211050:exon	30.0%
GCCAATAATTGTATTGTTGA+AGG	+	chr8_1:70413423-70413442	Msa1211050:intron	30.0%
CCCTTCAACAATACAATTAT+TGG	-	chr8_1:70413427-70413446	None:intergenic	30.0%
AACATGATTGTTTCGATATG+GGG	+	chr8_1:70413451-70413470	Msa1211050:intron	30.0%
GATACTGGATTTACTGTAAA+TGG	+	chr8_1:70413617-70413636	Msa1211050:CDS	30.0%
GTAAATGGAGTTGAATATGA+AGG	+	chr8_1:70413632-70413651	Msa1211050:CDS	30.0%
TTGGAAATTTGATCATGTCT+TGG	+	chr8_1:70413669-70413688	Msa1211050:CDS	30.0%
TTCCACTTTAAACTTCTTAG+CGG	-	chr8_1:70414104-70414123	None:intergenic	30.0%
TACCTCTTAATCTCTCTTAA+TGG	+	chr8_1:70414411-70414430	Msa1211050:intron	30.0%
TACCATTAAGAGAGATTAAG+AGG	-	chr8_1:70414416-70414435	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	70412924	70417314	70412924	ID=Msa1211050;Name=Msa1211050
chr8_1	mRNA	70412924	70417314	70412924	ID=Msa1211050-mRNA-1;Parent=Msa1211050;Name=Msa1211050-mRNA-1;_AED=0.05;_eAED=0.05;_QI=98\|1\|1\|1\|0.75\|1\|5\|530\|179
chr8_1	exon	70412924	70413221	70412924	ID=Msa1211050-mRNA-1:exon:23545;Parent=Msa1211050-mRNA-1
chr8_1	exon	70413610	70413726	70413610	ID=Msa1211050-mRNA-1:exon:23546;Parent=Msa1211050-mRNA-1
chr8_1	exon	70415048	70415082	70415048	ID=Msa1211050-mRNA-1:exon:23547;Parent=Msa1211050-mRNA-1
chr8_1	exon	70415578	70415678	70415578	ID=Msa1211050-mRNA-1:exon:23548;Parent=Msa1211050-mRNA-1
chr8_1	exon	70416698	70417314	70416698	ID=Msa1211050-mRNA-1:exon:23549;Parent=Msa1211050-mRNA-1
chr8_1	five_prime_UTR	70412924	70413021	70412924	ID=Msa1211050-mRNA-1:five_prime_utr;Parent=Msa1211050-mRNA-1
chr8_1	CDS	70413022	70413221	70413022	ID=Msa1211050-mRNA-1:cds;Parent=Msa1211050-mRNA-1
chr8_1	CDS	70413610	70413726	70413610	ID=Msa1211050-mRNA-1:cds;Parent=Msa1211050-mRNA-1
chr8_1	CDS	70415048	70415082	70415048	ID=Msa1211050-mRNA-1:cds;Parent=Msa1211050-mRNA-1
chr8_1	CDS	70415578	70415678	70415578	ID=Msa1211050-mRNA-1:cds;Parent=Msa1211050-mRNA-1
chr8_1	CDS	70416698	70416784	70416698	ID=Msa1211050-mRNA-1:cds;Parent=Msa1211050-mRNA-1
chr8_1	three_prime_UTR	70416785	70417314	70416785	ID=Msa1211050-mRNA-1:three_prime_utr;Parent=Msa1211050-mRNA-1

Gene Sequence

>Msa1211050

ATGGCTGTTCGTCAAAGAGCAGTAACAGCACTTCCAAACCTCATACGCAATCTCCGCAACGAACAACCTCTCAAGCTCAACAAACCTTCTCCACATAACAATCATCCCAATTCCTTGCCCTCTCTCCGTCGCGCTTTCTCTTTATACGATCAGATCAATCTCATTGACAATGTCCCCGAAGACCAACTCCGTTTTCAAGGGTTTAAAGATACTGGATTTACTGTAAATGGAGTTGAATATGAAGGAAGTTTGCTTTGTGTTGGAAATTTGATCATGTCTTGGAAACCAAACAAGTTTTCAGAGATCACTGCTGATAGCTTGTCACTCTTTCAAATTGTCCGCCCTATTCCAGAGATTCTGATTATTGGCTGTGGAAGGAGCATTCAACATGTTGATCCTGAACTTAGACGGTTTATTCGGTCTACTGGCATGAAGTTAGAAGCTGTTGACTCGAGAAATGCAGCATCAACTTACAACATACTAAATGAAGAAGGTCGCATTGTGGCTGCCGCACTTCTTCCATATGGAGTTACTTCATGA

Protein sequence

>Msa1211050

MAVRQRAVTALPNLIRNLRNEQPLKLNKPSPHNNHPNSLPSLRRAFSLYDQINLIDNVPEDQLRFQGFKDTGFTVNGVEYEGSLLCVGNLIMSWKPNKFSEITADSLSLFQIVRPIPEILIIGCGRSIQHVDPELRRFIRSTGMKLEAVDSRNAASTYNILNEEGRIVAAALLPYGVTS*