AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0458010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAGCTGGCAATCTATTA+TGG 0.259534 3_3:-53164744 None:intergenic
CCCTTTCATCTCTTGGTCCT+TGG 0.309835 3_3:-53166069 None:intergenic
ATATTCAGATGGCAAGGTTT+TGG 0.323652 3_3:+53166128 Msa0458010:CDS
ATTGTAGTAGGTGCCATTAC+AGG 0.330295 3_3:-53168119 Msa0458020:CDS
TTTAAAATTTCAAGAAGTTA+TGG 0.331787 3_3:+53165586 Msa0458010:intron
ACTGCTGCAGCATTCATAAA+TGG 0.345573 3_3:-53168083 Msa0458020:CDS
ATGAAAGGGATTACATATTC+AGG 0.353937 3_3:+53166083 Msa0458010:CDS
ATCTCTTGGTCCTTGGTGCA+TGG 0.358552 3_3:-53166062 None:intergenic
AGGTGAGGATTCCCCTCCTT+TGG 0.395511 3_3:+53164777 Msa0458010:CDS
GATTCTTACCGTTGTTCCCT+TGG 0.396543 3_3:-53168229 Msa0458020:intron
TGGTCCTTGGTGCATGGACC+AGG 0.397324 3_3:-53166056 None:intergenic
TTGGCTTCACAATGTACCTT+CGG 0.403077 3_3:+53165871 Msa0458010:CDS
GGTTCCAAGCTAAAAGCTTT+CGG 0.407241 3_3:-53166035 None:intergenic
ACATTTGGAGGTGCAGTGAC+AGG 0.426302 3_3:-53168182 Msa0458020:CDS
ATGTAATCCCTTTCATCTCT+TGG 0.434355 3_3:-53166076 None:intergenic
CGCAATTGCAGCCCCTGTAA+TGG 0.440225 3_3:+53168106 Msa0458010:CDS
ATGTAATCCCTTCCTTCTCT+TGG 0.440627 3_3:-53166107 None:intergenic
AGGTGCAGTGACAGGGGCTC+TGG 0.442203 3_3:-53168174 Msa0458020:CDS
TGGCTTCACAATGTACCTTC+GGG 0.451934 3_3:+53165872 Msa0458010:CDS
CCAAGGACCAAGAGATGAAA+GGG 0.465404 3_3:+53166069 Msa0458010:CDS
GGAGATAAGTGCTGGTGGCT+TGG 0.470552 3_3:+53164798 Msa0458010:CDS
CCTCCTTTGGAGATAAGTGC+TGG 0.472627 3_3:+53164790 Msa0458010:CDS
ATAAGTGCTGGTGGCTTGGT+TGG 0.479016 3_3:+53164802 Msa0458010:CDS
AAGGCAGACAAGATTGTAGT+AGG 0.498217 3_3:-53168131 Msa0458020:CDS
CTGCAGCATTCATAAATGGC+TGG 0.512276 3_3:-53168079 Msa0458020:CDS
ATCTGCAGCCAACAATGAAA+AGG 0.513448 3_3:-53168150 Msa0458020:CDS
CCAGCTTCAGTTGTTATGAA+AGG 0.517495 3_3:+53164757 Msa0458010:CDS
TTGTAGTAGGTGCCATTACA+GGG 0.523008 3_3:-53168118 Msa0458020:CDS
TTCAGGACCAAGAGAAGGAA+GGG 0.533095 3_3:+53166100 Msa0458010:CDS
CATTTGGAGGTGCAGTGACA+GGG 0.546047 3_3:-53168181 Msa0458020:CDS
CCTTTCATAACAACTGAAGC+TGG 0.551985 3_3:-53164757 None:intergenic
TTCTGTACATCAAAAGACCA+AGG 0.570986 3_3:+53168212 Msa0458010:CDS
GATTACATATTCAGATGGCA+AGG 0.573196 3_3:+53166122 Msa0458010:CDS
TGTCACTGCACCTCCAAATG+TGG 0.580744 3_3:+53168184 Msa0458010:CDS
GAAGGGATTACATATTCAGA+TGG 0.593397 3_3:+53166117 Msa0458010:CDS
ATTCAGGACCAAGAGAAGGA+AGG 0.598423 3_3:+53166099 Msa0458010:CDS
TCTGTACATCAAAAGACCAA+GGG 0.599344 3_3:+53168213 Msa0458010:CDS
TTCAGTTGTTATGAAAGGTG+AGG 0.600368 3_3:+53164762 Msa0458010:CDS
CAGCACTTATCTCCAAAGGA+GGG 0.603145 3_3:-53164789 None:intergenic
ACCAAGGACCAAGAGATGAA+AGG 0.604348 3_3:+53166068 Msa0458010:CDS
CCAGCACTTATCTCCAAAGG+AGG 0.604759 3_3:-53164790 None:intergenic
AAAGACACAATCGCAACACA+TGG 0.606302 3_3:+53165899 Msa0458010:CDS
CCTTTGGAGATAAGTGCTGG+TGG 0.611887 3_3:+53164793 Msa0458010:CDS
ACATATTCAGGACCAAGAGA+AGG 0.621450 3_3:+53166095 Msa0458010:CDS
CCACCAGCACTTATCTCCAA+AGG 0.623610 3_3:-53164793 None:intergenic
AATCGCAACACATGGCGCAA+AGG 0.628239 3_3:+53165907 Msa0458010:CDS
AGCACTTATCTCCAAAGGAG+GGG 0.629195 3_3:-53164788 None:intergenic
ATTTGGAGGTGCAGTGACAG+GGG 0.640699 3_3:-53168180 Msa0458020:CDS
ATTGTGTCTTTAGCACCCGA+AGG 0.652031 3_3:-53165887 None:intergenic
TCAAAAGACCAAGGGAACAA+CGG 0.671316 3_3:+53168221 Msa0458010:CDS
GGAACCTGGTCCATGCACCA+AGG 0.700852 3_3:+53166052 Msa0458010:CDS
TGTAGTAGGTGCCATTACAG+GGG 0.704939 3_3:-53168117 Msa0458020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTATTGTATCTTTTA+CGG + chr3_3:53165683-53165702 Msa0458010:intron 10.0%
!! ATTTAATATGTTTAATATGT+CGG + chr3_3:53166351-53166370 Msa0458010:intron 10.0%
!! ACAAAAAAATATTAAAGAGA+AGG - chr3_3:53165180-53165199 None:intergenic 15.0%
!! ACAAAAAAATATTAAAGAGA+AGG - chr3_3:53165180-53165199 None:intergenic 15.0%
!! TTAAAAAACAAATATGCTTT+GGG - chr3_3:53165435-53165454 None:intergenic 15.0%
!! ATTAAAAAACAAATATGCTT+TGG - chr3_3:53165436-53165455 None:intergenic 15.0%
!! TATTGAATATACTAAAACTT+GGG - chr3_3:53165506-53165525 None:intergenic 15.0%
!! TTATTGAATATACTAAAACT+TGG - chr3_3:53165507-53165526 None:intergenic 15.0%
!!! AAGTTTTAGTATATTCAATA+AGG + chr3_3:53165506-53165525 Msa0458010:intron 15.0%
!!! AGTTTTAGTATATTCAATAA+GGG + chr3_3:53165507-53165526 Msa0458010:intron 15.0%
!!! TTTAAAATTTCAAGAAGTTA+TGG + chr3_3:53165586-53165605 Msa0458010:intron 15.0%
!! GATAATTTATAATTTCTTGA+TGG - chr3_3:53165654-53165673 None:intergenic 15.0%
!! GATACAATAATATTATAATG+AGG - chr3_3:53165678-53165697 None:intergenic 15.0%
!! AGAAATAGTTAACTATTAAA+GGG + chr3_3:53165730-53165749 Msa0458010:intron 15.0%
!!! TTCAGTTTTAGTTTATTATC+TGG + chr3_3:53164877-53164896 Msa0458010:intron 20.0%
!! ACAGTTAAACTATCTAATAA+AGG - chr3_3:53165104-53165123 None:intergenic 20.0%
!! TTATTAGATAGTTTAACTGT+TGG + chr3_3:53165104-53165123 Msa0458010:intron 20.0%
!! AACAAAAACAAATATGCTTT+GGG - chr3_3:53165289-53165308 None:intergenic 20.0%
!! ATTGAATATACTAAAACTTG+GGG - chr3_3:53165505-53165524 None:intergenic 20.0%
!! GAGAAATAGTTAACTATTAA+AGG + chr3_3:53165729-53165748 Msa0458010:intron 20.0%
!!! ATATTTTCCAAGTTTATTTC+AGG - chr3_3:53165816-53165835 None:intergenic 20.0%
!!! ATGTACTTTTTATTAGTGTT+TGG + chr3_3:53165852-53165871 Msa0458010:intron 20.0%
!! AGGAAAAAAAAAATTGATGA+AGG + chr3_3:53166874-53166893 Msa0458010:intron 20.0%
!!! TTCAGTATTTTTTAAAGCAA+CGG + chr3_3:53167696-53167715 Msa0458010:intron 20.0%
!! ATATATATCATGATTTCAAC+AGG + chr3_3:53167734-53167753 Msa0458010:intron 20.0%
!! TATATATCATGATTTCAACA+GGG + chr3_3:53167735-53167754 Msa0458010:intron 20.0%
!!! CGTTTTCATAAATATTATTG+CGG - chr3_3:53167808-53167827 Msa0458020:three_prime_UTR 20.0%
!! CATCAAAATTATTACATAAC+CGG + chr3_3:53167970-53167989 Msa0458010:intron 20.0%
!! TATTATTCCATGTTTGAAAA+GGG - chr3_3:53168027-53168046 Msa0458020:three_prime_UTR 20.0%
!! TTTTGAATCTGTTCTAATTG+TGG + chr3_3:53165207-53165226 Msa0458010:intron 25.0%
! CAACAAAAACAAATATGCTT+TGG - chr3_3:53165290-53165309 None:intergenic 25.0%
!! TTTTGAATCTGTTCTAATTG+TGG + chr3_3:53165207-53165226 Msa0458010:intron 25.0%
!!! GTTTTTTAATTCAGAGATCT+TGG + chr3_3:53165446-53165465 Msa0458010:intron 25.0%
! ATGAAAAGTGAAAAAGAAAG+AGG + chr3_3:53165539-53165558 Msa0458010:intron 25.0%
! GTTTATACTGATCATTGAAT+TGG + chr3_3:53165705-53165724 Msa0458010:intron 25.0%
! TGTTATCTGCAATATATCAT+AGG - chr3_3:53166199-53166218 None:intergenic 25.0%
! ACCAAGAGTAATACATTATA+GGG - chr3_3:53166251-53166270 None:intergenic 25.0%
! AACCAAGAGTAATACATTAT+AGG - chr3_3:53166252-53166271 None:intergenic 25.0%
! GTTCTATATATAGACATGTT+TGG + chr3_3:53166436-53166455 Msa0458010:intron 25.0%
! TATTGTTTGTATACTCTACT+AGG + chr3_3:53166475-53166494 Msa0458010:intron 25.0%
! ATTGTTTGTATACTCTACTA+GGG + chr3_3:53166476-53166495 Msa0458010:intron 25.0%
!!! AGATTTTTTATTATAACCGC+CGG + chr3_3:53166550-53166569 Msa0458010:intron 25.0%
!!! ATATCTTGTTTTGTAAACAC+TGG - chr3_3:53166632-53166651 None:intergenic 25.0%
! AGTGTTTACAAAACAAGATA+TGG + chr3_3:53166631-53166650 Msa0458010:intron 25.0%
! ATAGAGTTAACATTATTGAG+TGG + chr3_3:53166827-53166846 Msa0458010:intron 25.0%
! TAGAGTTAACATTATTGAGT+GGG + chr3_3:53166828-53166847 Msa0458010:intron 25.0%
!! ATTTGCTTGTTAGAAACTAT+AGG + chr3_3:53167543-53167562 Msa0458010:intron 25.0%
!! CTTTTAGTATAAAGAAACCT+GGG - chr3_3:53167883-53167902 Msa0458020:three_prime_UTR 25.0%
!! ACTTTTAGTATAAAGAAACC+TGG - chr3_3:53167884-53167903 Msa0458020:three_prime_UTR 25.0%
!! CGGTTATGTAATAATTTTGA+TGG - chr3_3:53167972-53167991 Msa0458020:three_prime_UTR 25.0%
! ATTATTCCATGTTTGAAAAG+GGG - chr3_3:53168026-53168045 Msa0458020:three_prime_UTR 25.0%
! CTATTATTCCATGTTTGAAA+AGG - chr3_3:53168028-53168047 Msa0458020:three_prime_UTR 25.0%
TTTCTATACACACAACTTCA+AGG + chr3_3:53165060-53165079 Msa0458010:intron 30.0%
TTCTATACACACAACTTCAA+GGG + chr3_3:53165061-53165080 Msa0458010:intron 30.0%
!! ATGCTTCTTCAATTGCTTTT+TGG - chr3_3:53165474-53165493 None:intergenic 30.0%
GAAGTTATGGTATTTGTCAT+CGG + chr3_3:53165599-53165618 Msa0458010:intron 30.0%
TCAATGACCTGAAATAAACT+TGG + chr3_3:53165806-53165825 Msa0458010:intron 30.0%
TTCACACAAAATGATATGCT+CGG - chr3_3:53165955-53165974 None:intergenic 30.0%
GCAAACTTTATCAAACTGAA+TGG - chr3_3:53165986-53166005 None:intergenic 30.0%
ATGAAAGGGATTACATATTC+AGG + chr3_3:53166083-53166102 Msa0458010:CDS 30.0%
TGATATATTGCAGATAACAG+TGG + chr3_3:53166200-53166219 Msa0458010:intron 30.0%
ACCCTATAATGTATTACTCT+TGG + chr3_3:53166247-53166266 Msa0458010:intron 30.0%
GGACTCTGAATTTATAAATG+AGG - chr3_3:53166385-53166404 None:intergenic 30.0%
!! TTGCTCTTTTTGAAAATGCT+TGG + chr3_3:53166510-53166529 Msa0458010:intron 30.0%
CTAGATAGCTATGTAAAGAT+AGG + chr3_3:53166577-53166596 Msa0458010:intron 30.0%
!! TAGAGGAGTCGATTTTTAAA+TGG - chr3_3:53166770-53166789 None:intergenic 30.0%
AGAGTTAACATTATTGAGTG+GGG + chr3_3:53166829-53166848 Msa0458010:intron 30.0%
AAGGCAAAAGAAAGCAATAA+AGG + chr3_3:53166893-53166912 Msa0458010:intron 30.0%
TTGTAGTTTCTTTGCTTCTT+AGG - chr3_3:53166982-53167001 None:intergenic 30.0%
ACATGACAGTCACAAATATT+TGG + chr3_3:53167089-53167108 Msa0458010:intron 30.0%
CATGACAGTCACAAATATTT+GGG + chr3_3:53167090-53167109 Msa0458010:intron 30.0%
! CAGATAATGAAGGAATTTTG+GGG - chr3_3:53167339-53167358 Msa0458020:three_prime_UTR 30.0%
! GCAGATAATGAAGGAATTTT+GGG - chr3_3:53167340-53167359 Msa0458020:three_prime_UTR 30.0%
! TGCAGATAATGAAGGAATTT+TGG - chr3_3:53167341-53167360 Msa0458020:three_prime_UTR 30.0%
! TAAAAGTGTGCAGATAATGA+AGG - chr3_3:53167349-53167368 Msa0458020:three_prime_UTR 30.0%
! TCATTATCTGCACACTTTTA+AGG + chr3_3:53167349-53167368 Msa0458010:intron 30.0%
AGTTTCTAACAAGCAAATGT+TGG - chr3_3:53167541-53167560 Msa0458020:three_prime_UTR 30.0%
TTGTTAGAAACTATAGGTAG+AGG + chr3_3:53167549-53167568 Msa0458010:intron 30.0%
TTTCTGTGTATATCCTAAAG+AGG - chr3_3:53167640-53167659 Msa0458020:three_prime_UTR 30.0%
AACGTTAAACTTTATTCACC+TGG + chr3_3:53167824-53167843 Msa0458010:intron 30.0%
AAATTTAAACATTAGCAGCC+AGG - chr3_3:53167845-53167864 Msa0458020:three_prime_UTR 30.0%
TTCAGTTGTTATGAAAGGTG+AGG + chr3_3:53164762-53164781 Msa0458010:CDS 35.0%
CTGTTAGTATGAAAATGCGA+AGG + chr3_3:53164924-53164943 Msa0458010:intron 35.0%
AAATGGAGGAATCAATCAAG+AGG - chr3_3:53165257-53165276 None:intergenic 35.0%
TTGGGTTAAAGAGAAAATGG+AGG - chr3_3:53165271-53165290 None:intergenic 35.0%
! GCTTTGGGTTAAAGAGAAAA+TGG - chr3_3:53165274-53165293 None:intergenic 35.0%
AAATGGAGGAATCAATCAAG+AGG - chr3_3:53165257-53165276 None:intergenic 35.0%
TTGGGTTAAAGAGAAAATGG+AGG - chr3_3:53165271-53165290 None:intergenic 35.0%
! GCTTTGGGTTAAAGAGAAAA+TGG - chr3_3:53165274-53165293 None:intergenic 35.0%
AACAACAATGATCCAAGTGA+AGG + chr3_3:53165753-53165772 Msa0458010:intron 35.0%
ACAACAATGATCCAAGTGAA+GGG + chr3_3:53165754-53165773 Msa0458010:intron 35.0%
TCACACAAAATGATATGCTC+GGG - chr3_3:53165954-53165973 None:intergenic 35.0%
ATGTAATCCCTTTCATCTCT+TGG - chr3_3:53166079-53166098 None:intergenic 35.0%
GAAGGGATTACATATTCAGA+TGG + chr3_3:53166117-53166136 Msa0458010:CDS 35.0%
GATTACATATTCAGATGGCA+AGG + chr3_3:53166122-53166141 Msa0458010:CDS 35.0%
! ATATTCAGATGGCAAGGTTT+TGG + chr3_3:53166128-53166147 Msa0458010:CDS 35.0%
AGACTTCTATTCACACTAGT+AGG - chr3_3:53166169-53166188 None:intergenic 35.0%
TTGCAGATAACAGTGGATAA+AGG + chr3_3:53166207-53166226 Msa0458010:intron 35.0%
!! AGTTGTTTCTCAATCACAAG+AGG - chr3_3:53166303-53166322 None:intergenic 35.0%
AACATAGACATATCCTCCAA+AGG - chr3_3:53166419-53166438 None:intergenic 35.0%
ACAAACAATATTCCCTGAAC+TGG - chr3_3:53166465-53166484 None:intergenic 35.0%
TACATAGCTATCTAGACAAC+CGG - chr3_3:53166572-53166591 None:intergenic 35.0%
ACTGTGTTTAAACTACGTAG+AGG - chr3_3:53166787-53166806 None:intergenic 35.0%
! GGTCTTTTGTAGGACAATTT+GGG + chr3_3:53166850-53166869 Msa0458010:intron 35.0%
! GTCTTTTGTAGGACAATTTG+GGG + chr3_3:53166851-53166870 Msa0458010:intron 35.0%
CAAAAGAAAGCAATAAAGGC+AGG + chr3_3:53166897-53166916 Msa0458010:intron 35.0%
TTGCTTCTTAGGAATAGACT+TGG - chr3_3:53166971-53166990 None:intergenic 35.0%
!! TAGTAGCTAGTTCTGTTGTT+TGG - chr3_3:53167049-53167068 None:intergenic 35.0%
ATTTGTGACTGTCATGTAGA+TGG - chr3_3:53167086-53167105 None:intergenic 35.0%
!! GCTTTTGATTACACCCTTTT+TGG + chr3_3:53167116-53167135 Msa0458010:intron 35.0%
ATCCAAACTCGTTCCAAAAA+GGG - chr3_3:53167132-53167151 Msa0458020:three_prime_UTR 35.0%
AGATGCTATGTCAGTATGTT+TGG + chr3_3:53167242-53167261 Msa0458010:intron 35.0%
CTCAGACATTAACACTGATA+TGG - chr3_3:53167292-53167311 Msa0458020:three_prime_UTR 35.0%
CATATCAGTGTTAATGTCTG+AGG + chr3_3:53167290-53167309 Msa0458010:intron 35.0%
TTCTAGATCATGAGATGAAG+GGG - chr3_3:53167785-53167804 Msa0458020:three_prime_UTR 35.0%
! GTTCTAGATCATGAGATGAA+GGG - chr3_3:53167786-53167805 Msa0458020:three_prime_UTR 35.0%
! TTCTGTCAATTCTTGGTCTT+TGG - chr3_3:53167920-53167939 Msa0458020:three_prime_UTR 35.0%
TTCAGCTTTCTGTCAATTCT+TGG - chr3_3:53167927-53167946 Msa0458020:three_prime_UTR 35.0%
TAAATGGCTGGAGTTTCTAA+TGG - chr3_3:53168070-53168089 Msa0458020:CDS 35.0%
GTACAGAAAAATGCCACATT+TGG - chr3_3:53168200-53168219 Msa0458020:intron 35.0%
TTCTGTACATCAAAAGACCA+AGG + chr3_3:53168212-53168231 Msa0458010:CDS 35.0%
TCTGTACATCAAAAGACCAA+GGG + chr3_3:53168213-53168232 Msa0458010:CDS 35.0%
CAATGTAGCTGAATTTGGAG+AGG - chr3_3:53164722-53164741 None:intergenic 40.0%
TGGAGCAATGTAGCTGAATT+TGG - chr3_3:53164727-53164746 None:intergenic 40.0%
CTGAAGCTGGCAATCTATTA+TGG - chr3_3:53164747-53164766 None:intergenic 40.0%
CCTTTCATAACAACTGAAGC+TGG - chr3_3:53164760-53164779 None:intergenic 40.0%
CCAGCTTCAGTTGTTATGAA+AGG + chr3_3:53164757-53164776 Msa0458010:CDS 40.0%
! TCTTTTAGGCATCTGGTGAT+AGG + chr3_3:53164832-53164851 Msa0458010:CDS 40.0%
GTATGAAAATGCGAAGGAGT+TGG + chr3_3:53164930-53164949 Msa0458010:intron 40.0%
! GCTTTGTTGCGAAAACTCAA+TGG - chr3_3:53165026-53165045 None:intergenic 40.0%
TTTCGCAACAAAGCAGCAAA+AGG + chr3_3:53165032-53165051 Msa0458010:intron 40.0%
CAACAATGATCCAAGTGAAG+GGG + chr3_3:53165755-53165774 Msa0458010:intron 40.0%
TTAACATGTTCCCCTTCACT+TGG - chr3_3:53165768-53165787 None:intergenic 40.0%
TTGGCTTCACAATGTACCTT+CGG + chr3_3:53165871-53165890 Msa0458010:CDS 40.0%
AAAGACACAATCGCAACACA+TGG + chr3_3:53165899-53165918 Msa0458010:CDS 40.0%
GGTTCCAAGCTAAAAGCTTT+CGG - chr3_3:53166038-53166057 None:intergenic 40.0%
!!! AAAGCTTTTAGCTTGGAACC+TGG + chr3_3:53166038-53166057 Msa0458010:CDS 40.0%
ACATATTCAGGACCAAGAGA+AGG + chr3_3:53166095-53166114 Msa0458010:CDS 40.0%
ATGTAATCCCTTCCTTCTCT+TGG - chr3_3:53166110-53166129 None:intergenic 40.0%
!! AAGGTTTTGGCAAGAAGGAA+AGG + chr3_3:53166141-53166160 Msa0458010:CDS 40.0%
AGCTATGTAAAGATAGGCCA+TGG + chr3_3:53166583-53166602 Msa0458010:intron 40.0%
GCTATGTAAAGATAGGCCAT+GGG + chr3_3:53166584-53166603 Msa0458010:intron 40.0%
! TATTGAGTGGGGTCTTTTGT+AGG + chr3_3:53166840-53166859 Msa0458010:intron 40.0%
! GGGTCTTTTGTAGGACAATT+TGG + chr3_3:53166849-53166868 Msa0458010:intron 40.0%
! TTTTGTAGGACAATTTGGGG+AGG + chr3_3:53166854-53166873 Msa0458010:intron 40.0%
AAAGCAATAAAGGCAGGAGA+GGG + chr3_3:53166903-53166922 Msa0458010:intron 40.0%
GCGTAACCTCTTTCTGTATA+GGG - chr3_3:53166935-53166954 None:intergenic 40.0%
GTTTGGCAGCTTAGTTTCTT+TGG - chr3_3:53167032-53167051 None:intergenic 40.0%
CATCCAAACTCGTTCCAAAA+AGG - chr3_3:53167133-53167152 Msa0458020:three_prime_UTR 40.0%
CTTTGCCAATGTGAGTCTTA+GGG + chr3_3:53167181-53167200 Msa0458010:intron 40.0%
AAAAGCCCTAAGACTCACAT+TGG - chr3_3:53167189-53167208 Msa0458020:three_prime_UTR 40.0%
!!! TATCTGCACACTTTTAAGGC+TGG + chr3_3:53167353-53167372 Msa0458010:intron 40.0%
AGAGTTATAGGTGGAAAGTC+TGG - chr3_3:53167402-53167421 Msa0458020:three_prime_UTR 40.0%
AGTCTGGGCAAAAGAGTTAT+AGG - chr3_3:53167414-53167433 Msa0458020:three_prime_UTR 40.0%
GTGGATGAAGATTTGAGTCT+GGG - chr3_3:53167429-53167448 Msa0458020:three_prime_UTR 40.0%
TGTGGATGAAGATTTGAGTC+TGG - chr3_3:53167430-53167449 Msa0458020:three_prime_UTR 40.0%
ATCTTCATCCACAGAAGTGA+CGG + chr3_3:53167437-53167456 Msa0458010:intron 40.0%
GAACAGCCAAAGACAAAACT+GGG + chr3_3:53167460-53167479 Msa0458010:intron 40.0%
AACAGCCAAAGACAAAACTG+GGG + chr3_3:53167461-53167480 Msa0458010:intron 40.0%
CACCTATTCCATTGGAATCA+AGG - chr3_3:53167584-53167603 Msa0458020:three_prime_UTR 40.0%
TCTAAGCAAGCATCCTCTTT+AGG + chr3_3:53167624-53167643 Msa0458010:intron 40.0%
!! GGTTCTAGATCATGAGATGA+AGG - chr3_3:53167787-53167806 Msa0458020:three_prime_UTR 40.0%
! GTCTCTTTTGTCTGTCTTAC+CGG - chr3_3:53167992-53168011 Msa0458020:three_prime_UTR 40.0%
TTGAAAAGGGGTTGCACATT+TGG - chr3_3:53168014-53168033 Msa0458020:three_prime_UTR 40.0%
ACTGCTGCAGCATTCATAAA+TGG - chr3_3:53168086-53168105 Msa0458020:CDS 40.0%
!! TTGTAGTAGGTGCCATTACA+GGG - chr3_3:53168121-53168140 Msa0458020:CDS 40.0%
!! ATTGTAGTAGGTGCCATTAC+AGG - chr3_3:53168122-53168141 Msa0458020:CDS 40.0%
AAGGCAGACAAGATTGTAGT+AGG - chr3_3:53168134-53168153 Msa0458020:CDS 40.0%
! CTTGTCTGCCTTTTCATTGT+TGG + chr3_3:53168142-53168161 Msa0458010:CDS 40.0%
ATCTGCAGCCAACAATGAAA+AGG - chr3_3:53168153-53168172 Msa0458020:CDS 40.0%
CAGAAAAATGCCACATTTGG+AGG - chr3_3:53168197-53168216 Msa0458020:intron 40.0%
TCAAAAGACCAAGGGAACAA+CGG + chr3_3:53168221-53168240 Msa0458010:CDS 40.0%
AGCACTTATCTCCAAAGGAG+GGG - chr3_3:53164791-53164810 None:intergenic 45.0%
CAGCACTTATCTCCAAAGGA+GGG - chr3_3:53164792-53164811 None:intergenic 45.0%
!!! TGGTTGGTGAGAGCTCTTTT+AGG + chr3_3:53164818-53164837 Msa0458010:CDS 45.0%
! TGAGAGCTCTTTTAGGCATC+TGG + chr3_3:53164825-53164844 Msa0458010:CDS 45.0%
TTGTGGATGCATACCTCTTG+AGG + chr3_3:53165224-53165243 Msa0458010:intron 45.0%
! AAGAGGTATGCATCCTCAAG+AGG - chr3_3:53165240-53165259 None:intergenic 45.0%
! GAAGGAAGCACATATGCTTC+AGG - chr3_3:53165325-53165344 None:intergenic 45.0%
TGGCTTCACAATGTACCTTC+GGG + chr3_3:53165872-53165891 Msa0458010:CDS 45.0%
ATTGTGTCTTTAGCACCCGA+AGG - chr3_3:53165890-53165909 None:intergenic 45.0%
!! ACAGCCGAAAGCTTTTAGCT+TGG + chr3_3:53166031-53166050 Msa0458010:intron 45.0%
ACCAAGGACCAAGAGATGAA+AGG + chr3_3:53166068-53166087 Msa0458010:CDS 45.0%
CCAAGGACCAAGAGATGAAA+GGG + chr3_3:53166069-53166088 Msa0458010:CDS 45.0%
ATTCAGGACCAAGAGAAGGA+AGG + chr3_3:53166099-53166118 Msa0458010:CDS 45.0%
TTCAGGACCAAGAGAAGGAA+GGG + chr3_3:53166100-53166119 Msa0458010:CDS 45.0%
!! ATGGCAAGGTTTTGGCAAGA+AGG + chr3_3:53166136-53166155 Msa0458010:CDS 45.0%
TATCCTCCAAAGGTCACTCT+GGG - chr3_3:53166409-53166428 None:intergenic 45.0%
ATATCCTCCAAAGGTCACTC+TGG - chr3_3:53166410-53166429 None:intergenic 45.0%
ACATGTTTGGACTCCAGTTC+AGG + chr3_3:53166449-53166468 Msa0458010:intron 45.0%
CATGTTTGGACTCCAGTTCA+GGG + chr3_3:53166450-53166469 Msa0458010:intron 45.0%
ATAGCTATCTAGACAACCGG+CGG - chr3_3:53166569-53166588 None:intergenic 45.0%
GACACTGCTACTAGACAACT+CGG + chr3_3:53166606-53166625 Msa0458010:intron 45.0%
GAAAGCAATAAAGGCAGGAG+AGG + chr3_3:53166902-53166921 Msa0458010:intron 45.0%
CCTCTTTCTGTATAGGGTTC+AGG - chr3_3:53166929-53166948 None:intergenic 45.0%
CCTGAACCCTATACAGAAAG+AGG + chr3_3:53166926-53166945 Msa0458010:intron 45.0%
GGCGTAACCTCTTTCTGTAT+AGG - chr3_3:53166936-53166955 None:intergenic 45.0%
!!! CACCCTTTTTGGAACGAGTT+TGG + chr3_3:53167127-53167146 Msa0458010:intron 45.0%
! CACATTGGCAAAGGTTCTGA+TGG - chr3_3:53167174-53167193 Msa0458020:three_prime_UTR 45.0%
CCTAAGACTCACATTGGCAA+AGG - chr3_3:53167183-53167202 Msa0458020:three_prime_UTR 45.0%
CCTTTGCCAATGTGAGTCTT+AGG + chr3_3:53167180-53167199 Msa0458010:intron 45.0%
! TGCTTCTTAGTCTTCCTACC+AGG + chr3_3:53167210-53167229 Msa0458010:intron 45.0%
ATAGCATCTAACACCAAGCC+TGG - chr3_3:53167231-53167250 Msa0458020:three_prime_UTR 45.0%
CTGGGCAAAAGAGTTATAGG+TGG - chr3_3:53167411-53167430 Msa0458020:three_prime_UTR 45.0%
TCTTCATCCACAGAAGTGAC+GGG + chr3_3:53167438-53167457 Msa0458010:intron 45.0%
GGAACAGCCAAAGACAAAAC+TGG + chr3_3:53167459-53167478 Msa0458010:intron 45.0%
!! TAGCACCCCAGTTTTGTCTT+TGG - chr3_3:53167469-53167488 Msa0458020:three_prime_UTR 45.0%
! GTGATCCAAGTTTGGTGGTT+CGG - chr3_3:53167497-53167516 Msa0458020:three_prime_UTR 45.0%
AAATGTTGGACTCTGTCTCG+GGG - chr3_3:53167527-53167546 Msa0458020:three_prime_UTR 45.0%
CAAATGTTGGACTCTGTCTC+GGG - chr3_3:53167528-53167547 Msa0458020:three_prime_UTR 45.0%
GCAAATGTTGGACTCTGTCT+CGG - chr3_3:53167529-53167548 Msa0458020:three_prime_UTR 45.0%
AACTATAGGTAGAGGCTGAG+AGG + chr3_3:53167557-53167576 Msa0458010:intron 45.0%
ACTATAGGTAGAGGCTGAGA+GGG + chr3_3:53167558-53167577 Msa0458010:intron 45.0%
GGCCTTGATTCCAATGGAAT+AGG + chr3_3:53167579-53167598 Msa0458010:intron 45.0%
GCAAGATGCACCTATTCCAT+TGG - chr3_3:53167592-53167611 Msa0458020:three_prime_UTR 45.0%
TTGAAAACCTGTTCATGCCC+AGG + chr3_3:53167863-53167882 Msa0458010:intron 45.0%
AAAGAAACCTGGGCATGAAC+AGG - chr3_3:53167873-53167892 Msa0458020:three_prime_UTR 45.0%
! GTGCAACCCCTTTTCAAACA+TGG + chr3_3:53168017-53168036 Msa0458010:intron 45.0%
CTGCAGCATTCATAAATGGC+TGG - chr3_3:53168082-53168101 Msa0458020:CDS 45.0%
!! TGTAGTAGGTGCCATTACAG+GGG - chr3_3:53168120-53168139 Msa0458020:CDS 45.0%
GATTCTTACCGTTGTTCCCT+TGG - chr3_3:53168232-53168251 Msa0458020:intron 45.0%
CCAGCACTTATCTCCAAAGG+AGG - chr3_3:53164793-53164812 None:intergenic 50.0%
! CCTCCTTTGGAGATAAGTGC+TGG + chr3_3:53164790-53164809 Msa0458010:CDS 50.0%
CCACCAGCACTTATCTCCAA+AGG - chr3_3:53164796-53164815 None:intergenic 50.0%
!! CCTTTGGAGATAAGTGCTGG+TGG + chr3_3:53164793-53164812 Msa0458010:CDS 50.0%
!! ATAAGTGCTGGTGGCTTGGT+TGG + chr3_3:53164802-53164821 Msa0458010:CDS 50.0%
GAAGGCAGCACATATGCTTC+AGG - chr3_3:53165162-53165181 None:intergenic 50.0%
AATCGCAACACATGGCGCAA+AGG + chr3_3:53165907-53165926 Msa0458010:CDS 50.0%
! ATCTCTTGGTCCTTGGTGCA+TGG - chr3_3:53166065-53166084 None:intergenic 50.0%
CCCTTTCATCTCTTGGTCCT+TGG - chr3_3:53166072-53166091 None:intergenic 50.0%
TGTCTAGTAGCAGTGTCCCA+TGG - chr3_3:53166603-53166622 None:intergenic 50.0%
ACAGAAAGAGGTTACGCCTG+AGG + chr3_3:53166938-53166957 Msa0458010:intron 50.0%
!! TAGACTTGGCTTGCTTCCTC+AGG - chr3_3:53166957-53166976 None:intergenic 50.0%
! CTTAGTCTTCCTACCAGGCT+TGG + chr3_3:53167215-53167234 Msa0458010:intron 50.0%
CATCTAACACCAAGCCTGGT+AGG - chr3_3:53167227-53167246 Msa0458020:three_prime_UTR 50.0%
!!! TTTTAAGGCTGGCTGCTGAG+TGG + chr3_3:53167364-53167383 Msa0458010:intron 50.0%
GAACACCGAACCACCAAACT+TGG + chr3_3:53167489-53167508 Msa0458010:intron 50.0%
TGAGAGGGCCTTGATTCCAA+TGG + chr3_3:53167573-53167592 Msa0458010:intron 50.0%
AAGGGGACGTGTTGTAAGGT+AGG - chr3_3:53167768-53167787 Msa0458020:three_prime_UTR 50.0%
GATGAAGGGGACGTGTTGTA+AGG - chr3_3:53167772-53167791 Msa0458020:three_prime_UTR 50.0%
! ATTTGGAGGTGCAGTGACAG+GGG - chr3_3:53168183-53168202 Msa0458020:CDS 50.0%
! CATTTGGAGGTGCAGTGACA+GGG - chr3_3:53168184-53168203 Msa0458020:CDS 50.0%
! ACATTTGGAGGTGCAGTGAC+AGG - chr3_3:53168185-53168204 Msa0458020:CDS 50.0%
TGTCACTGCACCTCCAAATG+TGG + chr3_3:53168184-53168203 Msa0458010:CDS 50.0%
AGGTGAGGATTCCCCTCCTT+TGG + chr3_3:53164777-53164796 Msa0458010:CDS 55.0%
!! GGAGATAAGTGCTGGTGGCT+TGG + chr3_3:53164798-53164817 Msa0458010:CDS 55.0%
AGTCCACCCAGAGTGACCTT+TGG + chr3_3:53166400-53166419 Msa0458010:intron 55.0%
GGGGTGTGATCCAAGTTTGG+TGG - chr3_3:53167502-53167521 Msa0458020:three_prime_UTR 55.0%
! GAGGGGGTGTGATCCAAGTT+TGG - chr3_3:53167505-53167524 Msa0458020:three_prime_UTR 55.0%
! GGTGCATCTTGCTCTAGACC+TGG + chr3_3:53167600-53167619 Msa0458010:intron 55.0%
GAGGATGCTTGCTTAGAGCC+AGG - chr3_3:53167621-53167640 Msa0458020:three_prime_UTR 55.0%
CGCAATTGCAGCCCCTGTAA+TGG + chr3_3:53168106-53168125 Msa0458010:CDS 55.0%
GGAACCTGGTCCATGCACCA+AGG + chr3_3:53166052-53166071 Msa0458010:CDS 60.0%
! TGGTCCTTGGTGCATGGACC+AGG - chr3_3:53166059-53166078 None:intergenic 60.0%
CCTCCAAAGGTCACTCTGGG+TGG - chr3_3:53166406-53166425 None:intergenic 60.0%
CCACCCAGAGTGACCTTTGG+AGG + chr3_3:53166403-53166422 Msa0458010:intron 60.0%
GCTGGCTGCTGAGTGGTTAC+TGG + chr3_3:53167371-53167390 Msa0458010:intron 60.0%
GGCTGTTCCCGTCACTTCTG+TGG - chr3_3:53167448-53167467 Msa0458020:three_prime_UTR 60.0%
TTGGACTCTGTCTCGGGGAG+GGG - chr3_3:53167522-53167541 Msa0458020:three_prime_UTR 60.0%
GTTGGACTCTGTCTCGGGGA+GGG - chr3_3:53167523-53167542 Msa0458020:three_prime_UTR 60.0%
TGTTGGACTCTGTCTCGGGG+AGG - chr3_3:53167524-53167543 Msa0458020:three_prime_UTR 60.0%
TGGACTCTGTCTCGGGGAGG+GGG - chr3_3:53167521-53167540 Msa0458020:three_prime_UTR 65.0%
AGGTGCAGTGACAGGGGCTC+TGG - chr3_3:53168177-53168196 Msa0458020:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 53164691 53168259 53164691 ID=Msa0458010;Name=Msa0458010
chr3_3 mRNA 53164691 53168259 53164691 ID=Msa0458010-mRNA-1;Parent=Msa0458010;Name=Msa0458010-mRNA-1;_AED=0.64;_eAED=0.64;_QI=0|0|0|0.57|1|1|7|0|174
chr3_3 exon 53164691 53164694 53164691 ID=Msa0458010-mRNA-1:exon:10325;Parent=Msa0458010-mRNA-1
chr3_3 exon 53164734 53164853 53164734 ID=Msa0458010-mRNA-1:exon:10326;Parent=Msa0458010-mRNA-1
chr3_3 exon 53165600 53165607 53165600 ID=Msa0458010-mRNA-1:exon:10327;Parent=Msa0458010-mRNA-1
chr3_3 exon 53165631 53165638 53165631 ID=Msa0458010-mRNA-1:exon:10328;Parent=Msa0458010-mRNA-1
chr3_3 exon 53165868 53165928 53165868 ID=Msa0458010-mRNA-1:exon:10329;Parent=Msa0458010-mRNA-1
chr3_3 exon 53166035 53166172 53166035 ID=Msa0458010-mRNA-1:exon:10330;Parent=Msa0458010-mRNA-1
chr3_3 exon 53168074 53168259 53168074 ID=Msa0458010-mRNA-1:exon:10331;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53164691 53164694 53164691 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53164734 53164853 53164734 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53165600 53165607 53165600 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53165631 53165638 53165631 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53165868 53165928 53165868 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53166035 53166172 53166035 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
chr3_3 CDS 53168074 53168259 53168074 ID=Msa0458010-mRNA-1:cds;Parent=Msa0458010-mRNA-1
Gene Sequence

>Msa0458010

ATGGCTACATTGCTCCATAATAGATTGCCAGCTTCAGTTGTTATGAAAGGTGAGGATTCCCCTCCTTTGGAGATAAGTGCTGGTGGCTTGGTTGGTGAGAGCTCTTTTAGGCATCTGGTGATAGAAGTTATGAGTATAATTGTTTGGCTTCACAATGTACCTTCGGGTGCTAAAGACACAATCGCAACACATGGCGCAAAGCCGAAAGCTTTTAGCTTGGAACCTGGTCCATGCACCAAGGACCAAGAGATGAAAGGGATTACATATTCAGGACCAAGAGAAGGAAGGGATTACATATTCAGATGGCAAGGTTTTGGCAAGAAGGAAAGGAACCTACTAAAACTCCAGCCATTTATGAATGCTGCAGCAGTCGCAATTGCAGCCCCTGTAATGGCACCTACTACAATCTTGTCTGCCTTTTCATTGTTGGCTGCAGATACCAGAGCCCCTGTCACTGCACCTCCAAATGTGGCATTTTTCTGTACATCAAAAGACCAAGGGAACAACGGTAAGAATCTCAACTGA
Protein sequence

>Msa0458010

MATLLHNRLPASVVMKGEDSPPLEISAGGLVGESSFRHLVIEVMSIIVWLHNVPSGAKDTIATHGAKPKAFSLEPGPCTKDQEMKGITYSGPREGRDYIFRWQGFGKKERNLLKLQPFMNAAAVAIAAPVMAPTTILSAFSLLAADTRAPVTAPPNVAFFCTSKDQGNNGKNLN*