AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670220 MtrunA17_Chr4g0034691 69.444 720 159 8 1 663 1 716 0.0 957
Msa0670220 MtrunA17_Chr4g0034721 74.144 584 84 12 108 663 40 584 0.0 810
Msa0670220 MtrunA17_Chr4g0034721 76.879 346 33 5 3 332 4 318 3.80e-172 503
Msa0670220 MtrunA17_Chr4g0034721 52.419 124 23 2 48 139 205 324 4.70e-26 113
Msa0670220 MtrunA17_Chr4g0034731 63.964 444 94 6 235 656 34 433 0.0 555
Msa0670220 MtrunA17_Chr4g0034731 52.036 221 66 7 1 217 1 185 6.00e-45 166
Msa0670220 MtrunA17_Chr4g0034731 59.459 148 37 3 196 332 42 177 1.82e-40 153
Msa0670220 MtrunA17_Chr4g0034731 48.768 203 34 9 120 311 42 185 4.59e-36 140
Msa0670220 MtrunA17_Chr4g0034451 64.686 303 86 4 365 663 249 534 5.06e-138 414
Msa0670220 MtrunA17_Chr3g0144391 39.539 521 253 19 169 658 19 508 2.75e-95 303
Msa0670220 MtrunA17_Chr3g0144411 36.976 549 272 18 145 658 32 541 3.21e-93 298
Msa0670220 MtrunA17_Chr6g0485051 67.606 213 64 2 452 663 1 209 8.51e-93 286
Msa0670220 MtrunA17_Chr3g0138231 46.903 226 115 4 440 662 50 273 1.88e-63 211
Msa0670220 MtrunA17_Chr4g0034821 46.575 219 112 4 440 655 75 291 2.08e-62 209
Msa0670220 MtrunA17_Chr3g0144451 47.032 219 111 4 440 655 59 275 2.15e-60 203
Msa0670220 MtrunA17_Chr3g0144421 44.091 220 117 4 440 656 87 303 9.97e-57 194
Msa0670220 MtrunA17_Chr4g0034051 72.414 87 24 0 577 663 1 87 3.58e-43 150
Msa0670220 MtrunA17_Chr3g0139431 39.394 264 139 9 405 655 66 321 6.10e-43 157
Msa0670220 MtrunA17_Chr1g0147271 39.726 219 122 6 440 654 171 383 1.22e-42 158
Msa0670220 MtrunA17_Chr1g0147261 37.500 216 126 5 440 653 89 297 2.20e-40 149
Msa0670220 MtrunA17_Chr1g0147291 37.500 216 128 5 440 654 56 265 3.98e-40 148
Msa0670220 MtrunA17_Chr1g0147251 36.916 214 127 5 440 653 35 240 4.87e-40 147
Msa0670220 MtrunA17_Chr1g0146631 37.156 218 127 6 440 654 113 323 5.21e-40 149
Msa0670220 MtrunA17_Chr1g0147331 37.156 218 127 6 440 654 113 323 2.18e-39 147
Msa0670220 MtrunA17_Chr1g0147321 36.744 215 129 5 440 653 27 235 2.55e-39 145
Msa0670220 MtrunA17_Chr1g0147301 35.909 220 130 6 440 654 70 283 2.45e-38 143
Msa0670220 MtrunA17_Chr1g0147311 36.364 220 129 6 440 654 141 354 1.02e-36 141
Msa0670220 MtrunA17_Chr1g0147241 37.368 190 112 4 464 653 128 310 4.05e-34 132
Msa0670220 MtrunA17_Chr4g0034711 71.765 85 24 0 419 503 13 97 5.85e-34 125
Msa0670220 MtrunA17_Chr4g0034021 37.838 222 102 9 278 478 50 256 3.53e-27 110
Msa0670220 MtrunA17_Chr8g0365401 33.505 194 117 5 464 653 438 623 1.11e-26 115
Msa0670220 MtrunA17_Chr8g0365381 24.954 549 297 20 204 653 108 640 1.84e-26 114
Msa0670220 MtrunA17_Chr5g0413591 31.959 194 120 6 464 653 441 626 1.81e-25 111
Msa0670220 MtrunA17_Chr3g0117791 30.909 220 138 6 440 653 413 624 6.99e-25 109
Msa0670220 MtrunA17_Chr8g0365361 31.414 191 125 5 464 653 207 392 1.25e-23 103
Msa0670220 MtrunA17_Chr4g0076231 32.911 158 101 4 497 653 3 156 1.69e-21 91.7
Msa0670220 MtrunA17_Chr4g0034031 60.714 56 22 0 499 554 1 56 5.75e-18 78.2
Msa0670220 MtrunA17_Chr8g0355331 36.667 150 83 7 506 655 284 421 6.06e-17 84.0
Msa0670220 MtrunA17_Chr8g0355311 34.868 152 83 7 506 655 53 190 8.81e-17 79.3
Msa0670220 MtrunA17_Chr2g0318931 32.579 221 115 11 440 655 79 270 1.42e-16 80.5
Msa0670220 MtrunA17_Chr5g0412991 28.191 188 122 4 474 653 226 408 2.70e-15 78.6
Msa0670220 MtrunA17_Chr5g0412991 28.144 167 108 3 474 632 18 180 2.16e-13 72.8
Msa0670220 MtrunA17_Chr5g0412981 28.191 188 122 4 474 653 226 408 2.95e-15 78.6
Msa0670220 MtrunA17_Chr8g0355841 33.333 150 88 7 506 655 372 509 4.03e-15 78.6
Msa0670220 MtrunA17_Chr8g0355861 34.667 150 86 7 506 655 123 260 9.81e-15 75.1
Msa0670220 MtrunA17_Chr5g0412951 27.128 188 124 4 474 653 25 207 9.91e-15 73.6
Msa0670220 MtrunA17_Chr1g0168781 34.667 150 87 6 506 655 121 259 4.06e-14 73.2
Msa0670220 MtrunA17_Chr8g0355871 30.719 153 90 7 506 656 85 223 1.37e-11 65.1
Msa0670220 MtrunA17_Chr5g0413021 36.585 82 50 2 572 653 48 127 2.64e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670220 AT1G49320.1 36.937 222 132 3 440 654 58 278 2.77e-48 171
Msa0670220 AT5G25610.1 36.986 219 128 6 440 653 176 389 3.91e-39 149
Msa0670220 AT1G60390.1 29.897 194 124 6 464 653 435 620 2.75e-23 105
Msa0670220 AT1G70370.2 31.282 195 122 7 464 654 437 623 8.57e-23 103
Msa0670220 AT1G70370.1 31.282 195 122 7 464 654 437 623 8.57e-23 103
Msa0670220 AT1G23760.1 28.351 194 129 6 464 654 433 619 3.70e-20 95.5

Find 213 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATTAGATTCATGGATA+TGG 0.205278 4_4:-12494656 None:intergenic
TTGTACCCTGTAGTGTAATC+TGG 0.218562 4_4:-12494516 None:intergenic
CCCATTGGATTCTGATTGTT+TGG 0.238831 4_4:-12495078 None:intergenic
CCCATTGGATTCTGATTGTT+TGG 0.238831 4_4:-12495219 None:intergenic
TTTACAGAAGTACACCATTT+TGG 0.240272 4_4:+12495889 Msa0670220:CDS
TTCATTGGATTCTTGTTGTT+TGG 0.269359 4_4:-12495273 None:intergenic
TTGTACCCGGTAGTGTAATC+TGG 0.274373 4_4:-12495140 None:intergenic
ATTTAGGCAGCTTAGCATTA+AGG 0.280064 4_4:+12496114 Msa0670220:CDS
ATGTACCCGTTAAGGTAATT+TGG 0.281112 4_4:-12495422 None:intergenic
TGTAGTGTAATCTGGGTTCT+TGG 0.293300 4_4:-12494508 None:intergenic
TTGGTTTACTGCCTCTGTAC+TGG 0.294469 4_4:-12494371 None:intergenic
GTGTATGAAGCTGGGTAATT+TGG 0.297089 4_4:-12494390 None:intergenic
ACCCAGATCATATTATATTT+AGG 0.313828 4_4:+12496098 Msa0670220:CDS
AATTTGGTTGCTTGGCTTCA+TGG 0.313905 4_4:-12495406 None:intergenic
TGTAGTGTAATCCGGGTTCT+TGG 0.321752 4_4:-12494736 None:intergenic
TGTAGTGTAATCCGGGTTCT+TGG 0.321752 4_4:-12494850 None:intergenic
TTAGCTTGAGGAGAATCTTC+AGG 0.325821 4_4:-12495698 None:intergenic
CTTGTACCCGGTAGTGTAAT+CGG 0.326452 4_4:-12495000 None:intergenic
GGTAGTGTAATCGGGGTTCT+TGG 0.328512 4_4:-12494991 None:intergenic
TGCCTAAATATAATATGATC+TGG 0.343870 4_4:-12496100 None:intergenic
GGGCCTTACATTACTCAATA+TGG 0.344598 4_4:+12494957 Msa0670220:CDS
GGAAGTTGTGTAAGGTTCAT+TGG 0.348116 4_4:-12495288 None:intergenic
TGATATATGCTTGTTGTGTT+TGG 0.352204 4_4:-12494418 None:intergenic
TTCATTAGATTCATGGATAT+GGG 0.352274 4_4:-12494655 None:intergenic
ATCATGTGATACTTCCAAAA+TGG 0.355384 4_4:-12495903 None:intergenic
TTGGTGGTGGTGTATGAAGC+TGG 0.361912 4_4:-12494399 None:intergenic
GGCAGTGTTGTAAGGTTCAT+TGG 0.362108 4_4:-12495180 None:intergenic
AGATCGTTTGAGGTGGTAGA+TGG 0.363028 4_4:-12495473 None:intergenic
GAATTGTACGTATCTGAATA+TGG 0.363198 4_4:+12495380 Msa0670220:CDS
CCCATTGGAATCCAGCTGTT+TGG 0.367673 4_4:-12494937 None:intergenic
GGGCCTTACATAACTCAATA+TGG 0.370659 4_4:+12495098 Msa0670220:CDS
GGGCCTTACATAACTCAATA+TGG 0.370659 4_4:+12495239 Msa0670220:CDS
GGTAGTGTAATCTGGGTTCT+TGG 0.376751 4_4:-12495132 None:intergenic
AGCAGTTGGTGAGGGTTTGT+TGG 0.377333 4_4:-12495861 None:intergenic
GTGATCCAAGTTAGGAGATT+GGG 0.384612 4_4:-12495504 None:intergenic
GAATTTGTTGACATGATCAT+TGG 0.389245 4_4:+12495809 Msa0670220:CDS
AGTCTCTGGCATTGATGTCT+TGG 0.390832 4_4:-12494290 None:intergenic
TTGTACCCTGTAGTGTAATC+CGG 0.392483 4_4:-12494630 None:intergenic
TTGTACCCTGTAGTGTAATC+CGG 0.392483 4_4:-12494744 None:intergenic
TTGTACCCTGTAGTGTAATC+CGG 0.392483 4_4:-12494858 None:intergenic
TTTCATTAGATTCATGGGTA+TGG 0.395484 4_4:-12494542 None:intergenic
TTTCATTAGATTCATGGGTA+TGG 0.395484 4_4:-12494770 None:intergenic
TATGTAATGACAAAAGTAAA+TGG 0.399290 4_4:-12495975 None:intergenic
CAGCAGATATGTACCCGTTA+AGG 0.400401 4_4:-12495430 None:intergenic
GCCATTTGGACACATCTGAT+TGG 0.403046 4_4:+12496074 Msa0670220:CDS
AGCAACCAAATTACCTTAAC+GGG 0.404392 4_4:+12495417 Msa0670220:CDS
GGCAGTGTTGTAAGGTTCAA+TGG 0.404622 4_4:-12495039 None:intergenic
GGATGACAAGCTACCCATTT+GGG 0.410770 4_4:-12495935 None:intergenic
TGTAAGGTCCACTGGATTCA+TGG 0.411259 4_4:-12494890 None:intergenic
TTCATTAGATTCATGGGTAT+GGG 0.412584 4_4:-12494541 None:intergenic
TTCATTAGATTCATGGGTAT+GGG 0.412584 4_4:-12494769 None:intergenic
TGTGAATCAGAAACCATTAC+AGG 0.414463 4_4:+12495746 Msa0670220:CDS
TGTAAGGTTCAATGGATTCA+TGG 0.414653 4_4:-12495031 None:intergenic
AGCTGTTGGAATTTGCCATT+TGG 0.418321 4_4:+12496060 Msa0670220:CDS
CAGCTCTACCTTCAAGTCTC+TGG 0.419943 4_4:-12494304 None:intergenic
TGACAAAAGTAAATGGCATA+TGG 0.422075 4_4:-12495968 None:intergenic
CTCTCCAAGTGAATTTATCA+TGG 0.423407 4_4:-12495723 None:intergenic
GGGGCCTTCTAGATCGTTTG+AGG 0.424265 4_4:-12495483 None:intergenic
GTTAAGGTAATTTGGTTGCT+TGG 0.424962 4_4:-12495414 None:intergenic
GTTCATTGGATGCATGGGTA+TGG 0.426590 4_4:-12495166 None:intergenic
GTCCACTGGATTCATGGGTA+TGG 0.427423 4_4:-12494884 None:intergenic
GGATCACACAGAAGCTTTCA+AGG 0.433713 4_4:+12495520 Msa0670220:CDS
AGTCTTTGTCTCCCCTGTAA+TGG 0.434357 4_4:-12495759 None:intergenic
TTGTACCCGGTAGTGTAATC+GGG 0.440503 4_4:-12494999 None:intergenic
AATTTGGATGATCTTTATGT+TGG 0.440932 4_4:+12495554 Msa0670220:CDS
TTACCATATTGAGTTATGTA+AGG 0.441136 4_4:-12495101 None:intergenic
TTACCATATTGAGTTATGTA+AGG 0.441136 4_4:-12495242 None:intergenic
GCTTCTGTGTGATCCAAGTT+AGG 0.443750 4_4:-12495512 None:intergenic
GTTCAATGGATTCATGGGTA+TGG 0.445281 4_4:-12495025 None:intergenic
TGGATGACAAGCTACCCATT+TGG 0.450677 4_4:-12495936 None:intergenic
AAATGGTGTACTTCTGTAAA+GGG 0.451382 4_4:-12495886 None:intergenic
ATGCTTGTTGTGTTTGGTGG+TGG 0.454019 4_4:-12494412 None:intergenic
AAGCAACCAAATTACCTTAA+CGG 0.454443 4_4:+12495416 Msa0670220:CDS
TACCCGTGAACCATCTTATG+TGG 0.455186 4_4:+12496167 Msa0670220:CDS
AAAATGGTGTACTTCTGTAA+AGG 0.455484 4_4:-12495887 None:intergenic
CATCAATGCCAGAGACTTGA+AGG 0.456451 4_4:+12494296 Msa0670220:CDS
TTACCATAGGCAGTGTTGTA+AGG 0.456880 4_4:-12495047 None:intergenic
TTACCATAGGCAGTGTTGTA+AGG 0.456880 4_4:-12495188 None:intergenic
TATATGCTTGTTGTGTTTGG+TGG 0.457052 4_4:-12494415 None:intergenic
ATGATATTGATGCTCCCAAA+TGG 0.459757 4_4:+12495921 Msa0670220:CDS
GAACCTTACAACACTGCCTA+TGG 0.460056 4_4:+12495185 Msa0670220:CDS
TACAGGGACTAGAGTGTTCA+AGG 0.461314 4_4:+12496000 Msa0670220:CDS
TTGAGTTATGTAAGGCCCAT+TGG 0.465944 4_4:-12495093 None:intergenic
TTGAGTTATGTAAGGCCCAT+TGG 0.465944 4_4:-12495234 None:intergenic
GAACCTTACAACACTGCCTA+TGG 0.469114 4_4:+12495044 Msa0670220:CDS
TTACCATATTGAGTAATGTA+AGG 0.470153 4_4:-12494960 None:intergenic
TGTACCCTGTAGTGTAATCT+GGG 0.474545 4_4:-12494515 None:intergenic
GAAGGTAGAGCTGCAAGATC+AGG 0.475278 4_4:+12494314 Msa0670220:CDS
TTACCATAGGAAGTTGTGTA+AGG 0.475470 4_4:-12495296 None:intergenic
GATCGTTTGAGGTGGTAGAT+GGG 0.479040 4_4:-12495472 None:intergenic
TAGCTTGAGGAGAATCTTCA+GGG 0.480432 4_4:-12495697 None:intergenic
GAACCTTACACAACTTCCTA+TGG 0.481632 4_4:+12495293 Msa0670220:CDS
CTGTACCCGCCAGTCATGTA+AGG 0.482850 4_4:-12494462 None:intergenic
CTGTACCCGCCAGTCATGTA+AGG 0.482850 4_4:-12494576 None:intergenic
CTGTATCCGCCAGTCATGTA+AGG 0.482850 4_4:-12494690 None:intergenic
CTGTACCCGCCAGTCATGTA+AGG 0.482850 4_4:-12494804 None:intergenic
GCATTGGAACCCACATAAGA+TGG 0.485432 4_4:-12496177 None:intergenic
GGAGATAAAATGAAAGCTGT+TGG 0.485902 4_4:+12496046 Msa0670220:CDS
GTGTTCAAGGTCTCACTTGT+CGG 0.486727 4_4:+12496013 Msa0670220:CDS
GGCAGTTATGTAAGGTCCAC+TGG 0.487353 4_4:-12494898 None:intergenic
CTTCCGTAGGCAGTTATGTA+AGG 0.488428 4_4:-12494906 None:intergenic
TGGTTTACTGCCTCTGTACT+GGG 0.490538 4_4:-12494370 None:intergenic
TGTGATCCAAGTTAGGAGAT+TGG 0.490761 4_4:-12495505 None:intergenic
GGGAGTTGTGACATTGCAAA+AGG 0.491952 4_4:-12495650 None:intergenic
GGTGGTGAGAATACTATGTT+TGG 0.495632 4_4:-12495840 None:intergenic
ACCACCTCAAACGATCTAGA+AGG 0.500418 4_4:+12495479 Msa0670220:CDS
GAGAAGAGTTGAAGAACACT+TGG 0.501370 4_4:-12495671 None:intergenic
TTGAGTAATGTAAGGCCCAT+TGG 0.501940 4_4:-12494952 None:intergenic
TTTATCATGGATTTAGCTTG+AGG 0.502246 4_4:-12495710 None:intergenic
TCCAATCAGATGTGTCCAAA+TGG 0.503560 4_4:-12496075 None:intergenic
CCACTTGGACGGGAAATTGA+AGG 0.503790 4_4:-12495589 None:intergenic
AAGAACCCAGATTACACTAC+AGG 0.507759 4_4:+12494510 Msa0670220:CDS
CTAAGCGAGCCTTACATGAC+TGG 0.509211 4_4:+12494453 Msa0670220:CDS
CTAAGCGAGCCTTACATGAC+TGG 0.509211 4_4:+12494567 Msa0670220:CDS
CTAAGCGAGCCTTACATGAC+TGG 0.509211 4_4:+12494795 Msa0670220:CDS
ATCGTTTGAGGTGGTAGATG+GGG 0.509770 4_4:-12495471 None:intergenic
AAGAACCCGGATTACACTAC+AGG 0.510637 4_4:+12494738 Msa0670220:CDS
AAGAACCCGGATTACACTAC+AGG 0.510637 4_4:+12494852 Msa0670220:CDS
CTAATCGAGCCTTACATGAC+TGG 0.514028 4_4:+12494681 Msa0670220:CDS
CTGTGTATGAGTGTTATATC+CGG 0.518790 4_4:-12495348 None:intergenic
TTCATTGGATGCATGGGTAT+GGG 0.519593 4_4:-12495165 None:intergenic
TGGTGGTGGTGTATGAAGCT+GGG 0.523140 4_4:-12494398 None:intergenic
GCCTAAATATAATATGATCT+GGG 0.525219 4_4:-12496099 None:intergenic
TGTACCCTGTAGTGTAATCC+GGG 0.526516 4_4:-12494629 None:intergenic
TCCACTGGATTCATGGGTAT+GGG 0.527581 4_4:-12494883 None:intergenic
CCTCTGTACTGGGTAATGTA+TGG 0.527732 4_4:-12494360 None:intergenic
TTCAATGGATTCATGGGTAT+GGG 0.529636 4_4:-12495024 None:intergenic
TCGTTTGAGGTGGTAGATGG+GGG 0.531816 4_4:-12495470 None:intergenic
TGTACCCGGTAGTGTAATCT+GGG 0.532124 4_4:-12495139 None:intergenic
GTAAGGTTCAATGGATTCAT+GGG 0.532589 4_4:-12495030 None:intergenic
TTTGGCTTCATGTTTACCAT+AGG 0.532676 4_4:-12495060 None:intergenic
TTTGGCTTCATGTTTACCAT+AGG 0.532676 4_4:-12495201 None:intergenic
AGTTGGTGAGGGTTTGTTGG+TGG 0.534776 4_4:-12495858 None:intergenic
CTTAACGGGTACATATCTGC+TGG 0.538096 4_4:+12495431 Msa0670220:CDS
AAAGGAGAAACCACTTGGAC+GGG 0.539970 4_4:-12495599 None:intergenic
TTCTGTAAAGGGACAGCAGT+TGG 0.543533 4_4:-12495875 None:intergenic
ACACACTGGAGAGTTCTTCC+CGG 0.545365 4_4:-12496137 None:intergenic
AAGAACCCAGATTACACTAC+CGG 0.545560 4_4:+12495134 Msa0670220:CDS
TGATATTGATGCTCCCAAAT+GGG 0.545823 4_4:+12495922 Msa0670220:CDS
TCTGCTGGAAATGACCCAAA+AGG 0.545928 4_4:+12495446 Msa0670220:CDS
TTTGGCTTCATGTCTTCCGT+AGG 0.546060 4_4:-12494919 None:intergenic
AAGACATGAAGCCAAACAGC+TGG 0.547509 4_4:+12494926 Msa0670220:CDS
GGCAGCTTAGCATTAAGGCC+GGG 0.548154 4_4:+12496119 Msa0670220:CDS
GTAAGGTCCACTGGATTCAT+GGG 0.548211 4_4:-12494889 None:intergenic
AATCAACAAAGTTACAATAC+CGG 0.548351 4_4:+12495329 Msa0670220:CDS
AACCCACATAAGATGGTTCA+CGG 0.548650 4_4:-12496170 None:intergenic
AAGAACCCCGATTACACTAC+CGG 0.549721 4_4:+12494993 Msa0670220:CDS
CCAAACAATCAGAATCCAAT+GGG 0.549989 4_4:+12495078 Msa0670220:CDS
CCAAACAATCAGAATCCAAT+GGG 0.549989 4_4:+12495219 Msa0670220:CDS
TGTACCCTGTAGTGTAATCC+GGG 0.551924 4_4:-12494743 None:intergenic
TGTACCCTGTAGTGTAATCC+GGG 0.551924 4_4:-12494857 None:intergenic
AGGCAGCTTAGCATTAAGGC+CGG 0.553688 4_4:+12496118 Msa0670220:CDS
GGCGGGTACAGACTTGCATA+TGG 0.556538 4_4:+12494474 Msa0670220:CDS
GGCGGGTACAGACTTGCATA+TGG 0.556538 4_4:+12494588 Msa0670220:CDS
GGCGGATACAGACTTGCATA+TGG 0.556538 4_4:+12494702 Msa0670220:CDS
GGCGGGTACAGACTTGCATA+TGG 0.556538 4_4:+12494816 Msa0670220:CDS
GCCAAACAGCTGGATTCCAA+TGG 0.556982 4_4:+12494936 Msa0670220:CDS
TGTAAGGTTCATTGGATGCA+TGG 0.559989 4_4:-12495172 None:intergenic
TTGTGACATTGCAAAAGGAA+TGG 0.560283 4_4:-12495645 None:intergenic
CCAAACAGCTGGATTCCAAT+GGG 0.560358 4_4:+12494937 Msa0670220:CDS
ATTTGCTTCTTGTTTACCAT+AGG 0.564495 4_4:-12495309 None:intergenic
CGGATATAACACTCATACAC+AGG 0.565785 4_4:+12495349 Msa0670220:CDS
AGAGAAAAGGAGAAACCACT+TGG 0.566102 4_4:-12495604 None:intergenic
CACACAGAAGCTTTCAAGGT+AGG 0.566929 4_4:+12495524 Msa0670220:CDS
TCACTTGTCGGTGATGCGAA+TGG 0.570327 4_4:+12496025 Msa0670220:CDS
ATGGGTATGGGTCTTGTACC+CGG 0.573227 4_4:-12495012 None:intergenic
ATGGGTATGGGTCTTGTACC+CGG 0.577866 4_4:-12495153 None:intergenic
AAAAGGAGAAACCACTTGGA+CGG 0.578665 4_4:-12495600 None:intergenic
GCCAAACAATCAGAATCCAA+TGG 0.580573 4_4:+12495077 Msa0670220:CDS
GCCAAACAATCAGAATCCAA+TGG 0.580573 4_4:+12495218 Msa0670220:CDS
GAGCATGGACTCTAGAGAGT+TGG 0.580577 4_4:-12495786 None:intergenic
TGATCCAAGTTAGGAGATTG+GGG 0.581553 4_4:-12495503 None:intergenic
GTAAGGTTCATTGGATGCAT+GGG 0.583311 4_4:-12495171 None:intergenic
ACCCGTGAACCATCTTATGT+GGG 0.587697 4_4:+12496168 Msa0670220:CDS
CCTTCAATTTCCCGTCCAAG+TGG 0.588140 4_4:+12495589 Msa0670220:CDS
TAAACATGAAGCCAAGAACC+CGG 0.590476 4_4:+12494725 Msa0670220:CDS
TAAACATGAAGCCAAGAACC+CGG 0.590476 4_4:+12494839 Msa0670220:CDS
TAAAGGGACAGCAGTTGGTG+AGG 0.590824 4_4:-12495870 None:intergenic
GGACCTTACATAACTGCCTA+CGG 0.593355 4_4:+12494903 Msa0670220:CDS
TTTGTCATTACATAGCTACA+GGG 0.598347 4_4:+12495984 Msa0670220:CDS
CACTTGGACGGGAAATTGAA+GGG 0.598885 4_4:-12495588 None:intergenic
ACCCACATAAGATGGTTCAC+GGG 0.600337 4_4:-12496169 None:intergenic
AAATCCATGATAAATTCACT+TGG 0.603290 4_4:+12495719 Msa0670220:CDS
AAAGGGACAGCAGTTGGTGA+GGG 0.603981 4_4:-12495869 None:intergenic
GCTGCAAGATCAGGATCAAG+TGG 0.609867 4_4:+12494323 Msa0670220:CDS
ATGGCATATGGATATGAGAG+TGG 0.619183 4_4:-12495956 None:intergenic
GCCTTCTAGATCGTTTGAGG+TGG 0.619752 4_4:-12495480 None:intergenic
CATGTCAACAAATTCGAGCA+TGG 0.623516 4_4:-12495801 None:intergenic
GCGAGCCTTACATGACTGGC+GGG 0.626816 4_4:+12494457 Msa0670220:CDS
GCGAGCCTTACATGACTGGC+GGG 0.626816 4_4:+12494571 Msa0670220:CDS
GCGAGCCTTACATGACTGGC+GGG 0.626816 4_4:+12494799 Msa0670220:CDS
CGTTTGAGGTGGTAGATGGG+GGG 0.627717 4_4:-12495469 None:intergenic
AGAAGAGTTGAAGAACACTT+GGG 0.631035 4_4:-12495670 None:intergenic
GTGAATCAGAAACCATTACA+GGG 0.641222 4_4:+12495747 Msa0670220:CDS
AGCGAGCCTTACATGACTGG+CGG 0.648735 4_4:+12494456 Msa0670220:CDS
AGCGAGCCTTACATGACTGG+CGG 0.648735 4_4:+12494570 Msa0670220:CDS
AGCGAGCCTTACATGACTGG+CGG 0.648735 4_4:+12494798 Msa0670220:CDS
ATCGAGCCTTACATGACTGG+CGG 0.652711 4_4:+12494684 Msa0670220:CDS
ACCCATACCCATGAATCCAG+TGG 0.653332 4_4:+12494882 Msa0670220:CDS
AGAACCCCGATTACACTACC+GGG 0.655603 4_4:+12494994 Msa0670220:CDS
AGAACCCAGATTACACTACC+GGG 0.659254 4_4:+12495135 Msa0670220:CDS
ACGGGTAAGAAGTGACACAC+TGG 0.666639 4_4:-12496151 None:intergenic
TGTACCCGGTAGTGTAATCG+GGG 0.673181 4_4:-12494998 None:intergenic
AGAACCCAGATTACACTACA+GGG 0.673262 4_4:+12494511 Msa0670220:CDS
AAAACCCGGATTACACTACA+GGG 0.684827 4_4:+12494625 Msa0670220:CDS
GACGAACTCACTTCACATGG+TGG 0.684974 4_4:-12496212 None:intergenic
AGAACCCGGATTACACTACA+GGG 0.688478 4_4:+12494739 Msa0670220:CDS
AGAACCCGGATTACACTACA+GGG 0.688478 4_4:+12494853 Msa0670220:CDS
ATAGACGAACTCACTTCACA+TGG 0.696618 4_4:-12496215 None:intergenic
CCATACATTACCCAGTACAG+AGG 0.703245 4_4:+12494360 Msa0670220:CDS
AGAAGAAAATAACTACTGTG+AGG 0.714090 4_4:-12493036 None:intergenic
TGAATCAGAAACCATTACAG+GGG 0.729097 4_4:+12495748 Msa0670220:CDS
GATCCAAGTTAGGAGATTGG+GGG 0.743862 4_4:-12495502 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAAAATAAAAAAAT+GGG - chr4_4:12493671-12493690 None:intergenic 0.0%
!!! AATGTAAATATTTATTTTCA+AGG - chr4_4:12493338-12493357 None:intergenic 10.0%
!! TATATTGAAAGAAAAATTAT+AGG - chr4_4:12493563-12493582 None:intergenic 10.0%
!! TATATTAAACTTAACAAATA+AGG - chr4_4:12493872-12493891 None:intergenic 10.0%
!!! ATTATTTATTTTTTGGTTAA+AGG + chr4_4:12494111-12494130 Msa0670220:intron 10.0%
!!! CATTAATATTTTTTTTTTGA+CGG + chr4_4:12494195-12494214 Msa0670220:intron 10.0%
!!! ATTAATATTTTTTTTTTGAC+GGG + chr4_4:12494196-12494215 Msa0670220:intron 10.0%
!! TTTGATTAAGTTATTAGATA+CGG + chr4_4:12493137-12493156 Msa0670220:intron 15.0%
!!! TCCTATATTTTTTTTTTTTG+AGG + chr4_4:12493531-12493550 Msa0670220:intron 15.0%
!!! CCTATATTTTTTTTTTTTGA+GGG + chr4_4:12493532-12493551 Msa0670220:intron 15.0%
!! CAATAAATGATTATATAGAA+TGG - chr4_4:12493961-12493980 None:intergenic 15.0%
!! ATATAATAGTAATATCAACT+TGG + chr4_4:12494158-12494177 Msa0670220:intron 15.0%
!!! AGTAGTTATTTTCTTCTTTA+TGG + chr4_4:12493042-12493061 Msa0670220:CDS 20.0%
!! CCCTCAAAAAAAAAAAATAT+AGG - chr4_4:12493535-12493554 None:intergenic 20.0%
!! TTTGTTGAATATTGTGATTA+TGG + chr4_4:12493630-12493649 Msa0670220:intron 20.0%
!! TATGTAATGACAAAAGTAAA+TGG - chr4_4:12495978-12495997 None:intergenic 20.0%
! CTAAACAATGCAATTTCTAT+AGG + chr4_4:12493309-12493328 Msa0670220:intron 25.0%
! CTAAATCTACAAGATATAGA+TGG - chr4_4:12493721-12493740 None:intergenic 25.0%
!! AGTTAGAAGAAATTTTACGA+GGG - chr4_4:12493935-12493954 None:intergenic 25.0%
!! TAGCTTTTCATTAGATTCAT+GGG - chr4_4:12494550-12494569 None:intergenic 25.0%
!!! TTAGCTTTTCATTAGATTCA+TGG - chr4_4:12494551-12494570 None:intergenic 25.0%
! TTCATTAGATTCATGGATAT+GGG - chr4_4:12494658-12494677 None:intergenic 25.0%
! TTTCATTAGATTCATGGATA+TGG - chr4_4:12494659-12494678 None:intergenic 25.0%
!!! TTAGCTTTTCATTAGATTCA+TGG - chr4_4:12494551-12494570 None:intergenic 25.0%
!! TAGCTTTTCATTAGATTCAT+GGG - chr4_4:12494550-12494569 None:intergenic 25.0%
!!! TTAGCTTTTCATTAGATTCA+TGG - chr4_4:12494551-12494570 None:intergenic 25.0%
! TTACCATATTGAGTAATGTA+AGG - chr4_4:12494963-12494982 None:intergenic 25.0%
! TTACCATATTGAGTTATGTA+AGG - chr4_4:12495104-12495123 None:intergenic 25.0%
! TTACCATATTGAGTTATGTA+AGG - chr4_4:12495104-12495123 None:intergenic 25.0%
! AATCAACAAAGTTACAATAC+CGG + chr4_4:12495329-12495348 Msa0670220:CDS 25.0%
! AATTTGGATGATCTTTATGT+TGG + chr4_4:12495554-12495573 Msa0670220:CDS 25.0%
! AAATCCATGATAAATTCACT+TGG + chr4_4:12495719-12495738 Msa0670220:CDS 25.0%
! ACCCAGATCATATTATATTT+AGG + chr4_4:12496098-12496117 Msa0670220:CDS 25.0%
! GCCTAAATATAATATGATCT+GGG - chr4_4:12496102-12496121 None:intergenic 25.0%
! TGCCTAAATATAATATGATC+TGG - chr4_4:12496103-12496122 None:intergenic 25.0%
AGAAGAAAATAACTACTGTG+AGG - chr4_4:12493039-12493058 None:intergenic 30.0%
AATTGCACATAGTGATAGTT+GGG + chr4_4:12493071-12493090 Msa0670220:intron 30.0%
ATTAGATACGGAGAAAAAAG+AGG + chr4_4:12493149-12493168 Msa0670220:intron 30.0%
TTAGATACGGAGAAAAAAGA+GGG + chr4_4:12493150-12493169 Msa0670220:intron 30.0%
TAGTTCATTACAGGAAAATG+AGG - chr4_4:12493292-12493311 None:intergenic 30.0%
! TGCATTGTTTAGTTCATTAC+AGG - chr4_4:12493301-12493320 None:intergenic 30.0%
TTGAGTCACACAATTAAATG+TGG - chr4_4:12493595-12493614 None:intergenic 30.0%
! GAGTTAGAAGAAATTTTACG+AGG - chr4_4:12493936-12493955 None:intergenic 30.0%
!! TGATATATGCTTGTTGTGTT+TGG - chr4_4:12494421-12494440 None:intergenic 30.0%
TTCATTAGATTCATGGGTAT+GGG - chr4_4:12494544-12494563 None:intergenic 30.0%
TTTCATTAGATTCATGGGTA+TGG - chr4_4:12494545-12494564 None:intergenic 30.0%
TTCATTAGATTCATGGGTAT+GGG - chr4_4:12494544-12494563 None:intergenic 30.0%
TTTCATTAGATTCATGGGTA+TGG - chr4_4:12494545-12494564 None:intergenic 30.0%
!! TTCATTGGATTCTTGTTGTT+TGG - chr4_4:12495276-12495295 None:intergenic 30.0%
! ATTTGCTTCTTGTTTACCAT+AGG - chr4_4:12495312-12495331 None:intergenic 30.0%
GAATTGTACGTATCTGAATA+TGG + chr4_4:12495380-12495399 Msa0670220:CDS 30.0%
AAGCAACCAAATTACCTTAA+CGG + chr4_4:12495416-12495435 Msa0670220:CDS 30.0%
!! CAAGGTAGGATTTTTCAATT+TGG + chr4_4:12495538-12495557 Msa0670220:CDS 30.0%
! TCCTTTTCTCTCAAAAAAAG+AGG + chr4_4:12495616-12495635 Msa0670220:CDS 30.0%
!! TTTATCATGGATTTAGCTTG+AGG - chr4_4:12495713-12495732 None:intergenic 30.0%
!! GAATTTGTTGACATGATCAT+TGG + chr4_4:12495809-12495828 Msa0670220:CDS 30.0%
AAATGGTGTACTTCTGTAAA+GGG - chr4_4:12495889-12495908 None:intergenic 30.0%
AAAATGGTGTACTTCTGTAA+AGG - chr4_4:12495890-12495909 None:intergenic 30.0%
!! TTTACAGAAGTACACCATTT+TGG + chr4_4:12495889-12495908 Msa0670220:CDS 30.0%
ATCATGTGATACTTCCAAAA+TGG - chr4_4:12495906-12495925 None:intergenic 30.0%
TGACAAAAGTAAATGGCATA+TGG - chr4_4:12495971-12495990 None:intergenic 30.0%
! TTTTGTCATTACATAGCTAC+AGG + chr4_4:12495983-12496002 Msa0670220:CDS 30.0%
TTTGTCATTACATAGCTACA+GGG + chr4_4:12495984-12496003 Msa0670220:CDS 30.0%
CAATTGCACATAGTGATAGT+TGG + chr4_4:12493070-12493089 Msa0670220:intron 35.0%
ATTGCACATAGTGATAGTTG+GGG + chr4_4:12493072-12493091 Msa0670220:intron 35.0%
ATAAAAAAATGGGGTGACCA+TGG - chr4_4:12493661-12493680 None:intergenic 35.0%
ATCAACTTGGCTAATCAACT+TGG + chr4_4:12494171-12494190 Msa0670220:intron 35.0%
AATCTACAATGACAACAACG+AGG - chr4_4:12494268-12494287 None:intergenic 35.0%
TCGTTGTTGTCATTGTAGAT+TGG + chr4_4:12494267-12494286 Msa0670220:intron 35.0%
!! TATATGCTTGTTGTGTTTGG+TGG - chr4_4:12494418-12494437 None:intergenic 35.0%
TAAACATGAAGCCAAAAACC+CGG + chr4_4:12494611-12494630 Msa0670220:CDS 35.0%
! TTCAATGGATTCATGGGTAT+GGG - chr4_4:12495027-12495046 None:intergenic 35.0%
! GTAAGGTTCAATGGATTCAT+GGG - chr4_4:12495033-12495052 None:intergenic 35.0%
! TGTAAGGTTCAATGGATTCA+TGG - chr4_4:12495034-12495053 None:intergenic 35.0%
! TTTGGCTTCATGTTTACCAT+AGG - chr4_4:12494607-12494626 None:intergenic 35.0%
CCAAACAATCAGAATCCAAT+GGG + chr4_4:12495078-12495097 Msa0670220:CDS 35.0%
! TTTGGCTTCATGTTTACCAT+AGG - chr4_4:12494607-12494626 None:intergenic 35.0%
CCAAACAATCAGAATCCAAT+GGG + chr4_4:12495078-12495097 Msa0670220:CDS 35.0%
TTACCATAGGAAGTTGTGTA+AGG - chr4_4:12495299-12495318 None:intergenic 35.0%
CTGTGTATGAGTGTTATATC+CGG - chr4_4:12495351-12495370 None:intergenic 35.0%
GTTAAGGTAATTTGGTTGCT+TGG - chr4_4:12495417-12495436 None:intergenic 35.0%
AGCAACCAAATTACCTTAAC+GGG + chr4_4:12495417-12495436 Msa0670220:CDS 35.0%
ATGTACCCGTTAAGGTAATT+TGG - chr4_4:12495425-12495444 None:intergenic 35.0%
!!! GCCTCTTTTTTTGAGAGAAA+AGG - chr4_4:12495620-12495639 None:intergenic 35.0%
TTGTGACATTGCAAAAGGAA+TGG - chr4_4:12495648-12495667 None:intergenic 35.0%
AGAAGAGTTGAAGAACACTT+GGG - chr4_4:12495673-12495692 None:intergenic 35.0%
CTCTCCAAGTGAATTTATCA+TGG - chr4_4:12495726-12495745 None:intergenic 35.0%
TGTGAATCAGAAACCATTAC+AGG + chr4_4:12495746-12495765 Msa0670220:CDS 35.0%
GTGAATCAGAAACCATTACA+GGG + chr4_4:12495747-12495766 Msa0670220:CDS 35.0%
TGAATCAGAAACCATTACAG+GGG + chr4_4:12495748-12495767 Msa0670220:CDS 35.0%
ATGATATTGATGCTCCCAAA+TGG + chr4_4:12495921-12495940 Msa0670220:CDS 35.0%
TGATATTGATGCTCCCAAAT+GGG + chr4_4:12495922-12495941 Msa0670220:CDS 35.0%
GGAGATAAAATGAAAGCTGT+TGG + chr4_4:12496046-12496065 Msa0670220:CDS 35.0%
ATTTAGGCAGCTTAGCATTA+AGG + chr4_4:12496114-12496133 Msa0670220:CDS 35.0%
TTGTGATTATGGTGTAGCCA+TGG + chr4_4:12493641-12493660 Msa0670220:intron 40.0%
GTGTATGAAGCTGGGTAATT+TGG - chr4_4:12494393-12494412 None:intergenic 40.0%
TGTAGTGTAATCTGGGTTCT+TGG - chr4_4:12494511-12494530 None:intergenic 40.0%
AAGAACCCAGATTACACTAC+AGG + chr4_4:12494510-12494529 Msa0670220:CDS 40.0%
AGAACCCAGATTACACTACA+GGG + chr4_4:12494511-12494530 Msa0670220:CDS 40.0%
TGTACCCTGTAGTGTAATCT+GGG - chr4_4:12494518-12494537 None:intergenic 40.0%
TTGTACCCTGTAGTGTAATC+TGG - chr4_4:12494519-12494538 None:intergenic 40.0%
! TGTAGTGTAATCCGGGTTTT+TGG - chr4_4:12494625-12494644 None:intergenic 40.0%
AAAAACCCGGATTACACTAC+AGG + chr4_4:12494624-12494643 Msa0670220:CDS 40.0%
AAAACCCGGATTACACTACA+GGG + chr4_4:12494625-12494644 Msa0670220:CDS 40.0%
TTGTACCCTGTAGTGTAATC+CGG - chr4_4:12494519-12494538 None:intergenic 40.0%
TAAACATGAAGCCAAGAACC+CGG + chr4_4:12494497-12494516 Msa0670220:CDS 40.0%
TTGTACCCTGTAGTGTAATC+CGG - chr4_4:12494519-12494538 None:intergenic 40.0%
TAAACATGAAGCCAAGAACC+CGG + chr4_4:12494497-12494516 Msa0670220:CDS 40.0%
TTGTACCCTGTAGTGTAATC+CGG - chr4_4:12494519-12494538 None:intergenic 40.0%
TTGAGTAATGTAAGGCCCAT+TGG - chr4_4:12494955-12494974 None:intergenic 40.0%
GGGCCTTACATTACTCAATA+TGG + chr4_4:12494957-12494976 Msa0670220:CDS 40.0%
! GTTCAATGGATTCATGGGTA+TGG - chr4_4:12495028-12495047 None:intergenic 40.0%
TTACCATAGGCAGTGTTGTA+AGG - chr4_4:12495050-12495069 None:intergenic 40.0%
GCCAAACAATCAGAATCCAA+TGG + chr4_4:12495077-12495096 Msa0670220:CDS 40.0%
CCCATTGGATTCTGATTGTT+TGG - chr4_4:12495081-12495100 None:intergenic 40.0%
TTGAGTTATGTAAGGCCCAT+TGG - chr4_4:12495096-12495115 None:intergenic 40.0%
GGGCCTTACATAACTCAATA+TGG + chr4_4:12495098-12495117 Msa0670220:CDS 40.0%
AAGAACCCAGATTACACTAC+CGG + chr4_4:12494510-12494529 Msa0670220:CDS 40.0%
TTCATTGGATGCATGGGTAT+GGG - chr4_4:12495168-12495187 None:intergenic 40.0%
GTAAGGTTCATTGGATGCAT+GGG - chr4_4:12495174-12495193 None:intergenic 40.0%
TGTAAGGTTCATTGGATGCA+TGG - chr4_4:12495175-12495194 None:intergenic 40.0%
TTACCATAGGCAGTGTTGTA+AGG - chr4_4:12495050-12495069 None:intergenic 40.0%
GCCAAACAATCAGAATCCAA+TGG + chr4_4:12495077-12495096 Msa0670220:CDS 40.0%
CCCATTGGATTCTGATTGTT+TGG - chr4_4:12495081-12495100 None:intergenic 40.0%
TTGAGTTATGTAAGGCCCAT+TGG - chr4_4:12495096-12495115 None:intergenic 40.0%
GGGCCTTACATAACTCAATA+TGG + chr4_4:12495098-12495117 Msa0670220:CDS 40.0%
GGAAGTTGTGTAAGGTTCAT+TGG - chr4_4:12495291-12495310 None:intergenic 40.0%
GAACCTTACACAACTTCCTA+TGG + chr4_4:12495293-12495312 Msa0670220:CDS 40.0%
CGGATATAACACTCATACAC+AGG + chr4_4:12495349-12495368 Msa0670220:CDS 40.0%
AATTTGGTTGCTTGGCTTCA+TGG - chr4_4:12495409-12495428 None:intergenic 40.0%
TGATCCAAGTTAGGAGATTG+GGG - chr4_4:12495506-12495525 None:intergenic 40.0%
GTGATCCAAGTTAGGAGATT+GGG - chr4_4:12495507-12495526 None:intergenic 40.0%
TGTGATCCAAGTTAGGAGAT+TGG - chr4_4:12495508-12495527 None:intergenic 40.0%
AAAAGGAGAAACCACTTGGA+CGG - chr4_4:12495603-12495622 None:intergenic 40.0%
AGAGAAAAGGAGAAACCACT+TGG - chr4_4:12495607-12495626 None:intergenic 40.0%
GAGAAGAGTTGAAGAACACT+TGG - chr4_4:12495674-12495693 None:intergenic 40.0%
TAGCTTGAGGAGAATCTTCA+GGG - chr4_4:12495700-12495719 None:intergenic 40.0%
! TTAGCTTGAGGAGAATCTTC+AGG - chr4_4:12495701-12495720 None:intergenic 40.0%
CATGTCAACAAATTCGAGCA+TGG - chr4_4:12495804-12495823 None:intergenic 40.0%
GGTGGTGAGAATACTATGTT+TGG - chr4_4:12495843-12495862 None:intergenic 40.0%
ATGGCATATGGATATGAGAG+TGG - chr4_4:12495959-12495978 None:intergenic 40.0%
AGCTGTTGGAATTTGCCATT+TGG + chr4_4:12496060-12496079 Msa0670220:CDS 40.0%
TCCAATCAGATGTGTCCAAA+TGG - chr4_4:12496078-12496097 None:intergenic 40.0%
AACCCACATAAGATGGTTCA+CGG - chr4_4:12496173-12496192 None:intergenic 40.0%
CATAGTGATAGTTGGGGTCT+TGG + chr4_4:12493078-12493097 Msa0670220:intron 45.0%
CAGGAAAATGAGGAGAGTGA+AGG - chr4_4:12493282-12493301 None:intergenic 45.0%
!! AGTCTCTGGCATTGATGTCT+TGG - chr4_4:12494293-12494312 None:intergenic 45.0%
CATCAATGCCAGAGACTTGA+AGG + chr4_4:12494296-12494315 Msa0670220:CDS 45.0%
CCTCTGTACTGGGTAATGTA+TGG - chr4_4:12494363-12494382 None:intergenic 45.0%
CCATACATTACCCAGTACAG+AGG + chr4_4:12494360-12494379 Msa0670220:CDS 45.0%
TGGTTTACTGCCTCTGTACT+GGG - chr4_4:12494373-12494392 None:intergenic 45.0%
TTGGTTTACTGCCTCTGTAC+TGG - chr4_4:12494374-12494393 None:intergenic 45.0%
!! ATGCTTGTTGTGTTTGGTGG+TGG - chr4_4:12494415-12494434 None:intergenic 45.0%
TGTACCCTGTAGTGTAATCC+GGG - chr4_4:12494632-12494651 None:intergenic 45.0%
CTAATCGAGCCTTACATGAC+TGG + chr4_4:12494681-12494700 Msa0670220:CDS 45.0%
TGTAGTGTAATCCGGGTTCT+TGG - chr4_4:12494739-12494758 None:intergenic 45.0%
AAGAACCCGGATTACACTAC+AGG + chr4_4:12494738-12494757 Msa0670220:CDS 45.0%
AGAACCCGGATTACACTACA+GGG + chr4_4:12494739-12494758 Msa0670220:CDS 45.0%
TGTACCCTGTAGTGTAATCC+GGG - chr4_4:12494632-12494651 None:intergenic 45.0%
TGTAGTGTAATCCGGGTTCT+TGG - chr4_4:12494739-12494758 None:intergenic 45.0%
AAGAACCCGGATTACACTAC+AGG + chr4_4:12494738-12494757 Msa0670220:CDS 45.0%
AGAACCCGGATTACACTACA+GGG + chr4_4:12494739-12494758 Msa0670220:CDS 45.0%
TGTACCCTGTAGTGTAATCC+GGG - chr4_4:12494632-12494651 None:intergenic 45.0%
TCCACTGGATTCATGGGTAT+GGG - chr4_4:12494886-12494905 None:intergenic 45.0%
GTAAGGTCCACTGGATTCAT+GGG - chr4_4:12494892-12494911 None:intergenic 45.0%
TGTAAGGTCCACTGGATTCA+TGG - chr4_4:12494893-12494912 None:intergenic 45.0%
GGACCTTACATAACTGCCTA+CGG + chr4_4:12494903-12494922 Msa0670220:CDS 45.0%
CTTCCGTAGGCAGTTATGTA+AGG - chr4_4:12494909-12494928 None:intergenic 45.0%
! TTTGGCTTCATGTCTTCCGT+AGG - chr4_4:12494922-12494941 None:intergenic 45.0%
AAGACATGAAGCCAAACAGC+TGG + chr4_4:12494926-12494945 Msa0670220:CDS 45.0%
CCAAACAGCTGGATTCCAAT+GGG + chr4_4:12494937-12494956 Msa0670220:CDS 45.0%
AAGAACCCCGATTACACTAC+CGG + chr4_4:12494993-12495012 Msa0670220:CDS 45.0%
TTGTACCCGGTAGTGTAATC+GGG - chr4_4:12495002-12495021 None:intergenic 45.0%
CTTGTACCCGGTAGTGTAAT+CGG - chr4_4:12495003-12495022 None:intergenic 45.0%
GGCAGTGTTGTAAGGTTCAA+TGG - chr4_4:12495042-12495061 None:intergenic 45.0%
GAACCTTACAACACTGCCTA+TGG + chr4_4:12495044-12495063 Msa0670220:CDS 45.0%
GGTAGTGTAATCTGGGTTCT+TGG - chr4_4:12495135-12495154 None:intergenic 45.0%
AGAACCCAGATTACACTACC+GGG + chr4_4:12495135-12495154 Msa0670220:CDS 45.0%
TGTACCCGGTAGTGTAATCT+GGG - chr4_4:12495142-12495161 None:intergenic 45.0%
TTGTACCCGGTAGTGTAATC+TGG - chr4_4:12495002-12495021 None:intergenic 45.0%
GTTCATTGGATGCATGGGTA+TGG - chr4_4:12495169-12495188 None:intergenic 45.0%
GGCAGTGTTGTAAGGTTCAT+TGG - chr4_4:12495183-12495202 None:intergenic 45.0%
GAACCTTACAACACTGCCTA+TGG + chr4_4:12495044-12495063 Msa0670220:CDS 45.0%
CAGCAGATATGTACCCGTTA+AGG - chr4_4:12495433-12495452 None:intergenic 45.0%
! CTTAACGGGTACATATCTGC+TGG + chr4_4:12495431-12495450 Msa0670220:CDS 45.0%
TCTGCTGGAAATGACCCAAA+AGG + chr4_4:12495446-12495465 Msa0670220:CDS 45.0%
! ATCGTTTGAGGTGGTAGATG+GGG - chr4_4:12495474-12495493 None:intergenic 45.0%
! GATCGTTTGAGGTGGTAGAT+GGG - chr4_4:12495475-12495494 None:intergenic 45.0%
! AGATCGTTTGAGGTGGTAGA+TGG - chr4_4:12495476-12495495 None:intergenic 45.0%
ACCACCTCAAACGATCTAGA+AGG + chr4_4:12495479-12495498 Msa0670220:CDS 45.0%
GATCCAAGTTAGGAGATTGG+GGG - chr4_4:12495505-12495524 None:intergenic 45.0%
GCTTCTGTGTGATCCAAGTT+AGG - chr4_4:12495515-12495534 None:intergenic 45.0%
GGATCACACAGAAGCTTTCA+AGG + chr4_4:12495520-12495539 Msa0670220:CDS 45.0%
CACACAGAAGCTTTCAAGGT+AGG + chr4_4:12495524-12495543 Msa0670220:CDS 45.0%
CACTTGGACGGGAAATTGAA+GGG - chr4_4:12495591-12495610 None:intergenic 45.0%
AAAGGAGAAACCACTTGGAC+GGG - chr4_4:12495602-12495621 None:intergenic 45.0%
GGGAGTTGTGACATTGCAAA+AGG - chr4_4:12495653-12495672 None:intergenic 45.0%
AGTCTTTGTCTCCCCTGTAA+TGG - chr4_4:12495762-12495781 None:intergenic 45.0%
TTCTGTAAAGGGACAGCAGT+TGG - chr4_4:12495878-12495897 None:intergenic 45.0%
GGATGACAAGCTACCCATTT+GGG - chr4_4:12495938-12495957 None:intergenic 45.0%
TGGATGACAAGCTACCCATT+TGG - chr4_4:12495939-12495958 None:intergenic 45.0%
! TACAGGGACTAGAGTGTTCA+AGG + chr4_4:12496000-12496019 Msa0670220:CDS 45.0%
GTGTTCAAGGTCTCACTTGT+CGG + chr4_4:12496013-12496032 Msa0670220:CDS 45.0%
GCCATTTGGACACATCTGAT+TGG + chr4_4:12496074-12496093 Msa0670220:CDS 45.0%
TACCCGTGAACCATCTTATG+TGG + chr4_4:12496167-12496186 Msa0670220:CDS 45.0%
ACCCGTGAACCATCTTATGT+GGG + chr4_4:12496168-12496187 Msa0670220:CDS 45.0%
ACCCACATAAGATGGTTCAC+GGG - chr4_4:12496172-12496191 None:intergenic 45.0%
GCATTGGAACCCACATAAGA+TGG - chr4_4:12496180-12496199 None:intergenic 45.0%
!! GTGGCTTTTGAAGGTTGCAT+TGG - chr4_4:12496196-12496215 None:intergenic 45.0%
!!! TTATAATTATAACTTTTTTA+AGG + chr4_4:12493426-12493445 Msa0670220:intron 5.0%
!! TAAATAAAAATAAAAAAATG+GGG - chr4_4:12493670-12493689 None:intergenic 5.0%
!! GATAAATAAAAATAAAAAAA+TGG - chr4_4:12493672-12493691 None:intergenic 5.0%
!!! TTTTATTTTTTCTTTATATT+TGG + chr4_4:12493909-12493928 Msa0670220:intron 5.0%
!!! CAATATTATTATTTATTTTT+TGG + chr4_4:12494104-12494123 Msa0670220:intron 5.0%
TGATAGTTGGGGTCTTGGAG+AGG + chr4_4:12493083-12493102 Msa0670220:intron 50.0%
GATAGTTGGGGTCTTGGAGA+GGG + chr4_4:12493084-12493103 Msa0670220:intron 50.0%
ATAGTTGGGGTCTTGGAGAG+GGG + chr4_4:12493085-12493104 Msa0670220:intron 50.0%
CAGCTCTACCTTCAAGTCTC+TGG - chr4_4:12494307-12494326 None:intergenic 50.0%
GAAGGTAGAGCTGCAAGATC+AGG + chr4_4:12494314-12494333 Msa0670220:CDS 50.0%
GCTGCAAGATCAGGATCAAG+TGG + chr4_4:12494323-12494342 Msa0670220:CDS 50.0%
!! TGGTGGTGGTGTATGAAGCT+GGG - chr4_4:12494401-12494420 None:intergenic 50.0%
!! TTGGTGGTGGTGTATGAAGC+TGG - chr4_4:12494402-12494421 None:intergenic 50.0%
CTAAGCGAGCCTTACATGAC+TGG + chr4_4:12494453-12494472 Msa0670220:CDS 50.0%
CTAAGCGAGCCTTACATGAC+TGG + chr4_4:12494453-12494472 Msa0670220:CDS 50.0%
ATCGAGCCTTACATGACTGG+CGG + chr4_4:12494684-12494703 Msa0670220:CDS 50.0%
CTGTATCCGCCAGTCATGTA+AGG - chr4_4:12494693-12494712 None:intergenic 50.0%
GGCGGATACAGACTTGCATA+TGG + chr4_4:12494702-12494721 Msa0670220:CDS 50.0%
CTAAGCGAGCCTTACATGAC+TGG + chr4_4:12494453-12494472 Msa0670220:CDS 50.0%
ACCCATACCCATGAATCCAG+TGG + chr4_4:12494882-12494901 Msa0670220:CDS 50.0%
GTCCACTGGATTCATGGGTA+TGG - chr4_4:12494887-12494906 None:intergenic 50.0%
GGCAGTTATGTAAGGTCCAC+TGG - chr4_4:12494901-12494920 None:intergenic 50.0%
GCCAAACAGCTGGATTCCAA+TGG + chr4_4:12494936-12494955 Msa0670220:CDS 50.0%
CCCATTGGAATCCAGCTGTT+TGG - chr4_4:12494940-12494959 None:intergenic 50.0%
GGTAGTGTAATCGGGGTTCT+TGG - chr4_4:12494994-12495013 None:intergenic 50.0%
AGAACCCCGATTACACTACC+GGG + chr4_4:12494994-12495013 Msa0670220:CDS 50.0%
TGTACCCGGTAGTGTAATCG+GGG - chr4_4:12495001-12495020 None:intergenic 50.0%
! ATGGGTATGGGTCTTGTACC+CGG - chr4_4:12494532-12494551 None:intergenic 50.0%
! ATGGGTATGGGTCTTGTACC+CGG - chr4_4:12494532-12494551 None:intergenic 50.0%
! TCGTTTGAGGTGGTAGATGG+GGG - chr4_4:12495473-12495492 None:intergenic 50.0%
GCCTTCTAGATCGTTTGAGG+TGG - chr4_4:12495483-12495502 None:intergenic 50.0%
CCACTTGGACGGGAAATTGA+AGG - chr4_4:12495592-12495611 None:intergenic 50.0%
CCTTCAATTTCCCGTCCAAG+TGG + chr4_4:12495589-12495608 Msa0670220:CDS 50.0%
! GAGCATGGACTCTAGAGAGT+TGG - chr4_4:12495789-12495808 None:intergenic 50.0%
!! AGTTGGTGAGGGTTTGTTGG+TGG - chr4_4:12495861-12495880 None:intergenic 50.0%
!! AGCAGTTGGTGAGGGTTTGT+TGG - chr4_4:12495864-12495883 None:intergenic 50.0%
!! AAAGGGACAGCAGTTGGTGA+GGG - chr4_4:12495872-12495891 None:intergenic 50.0%
! TAAAGGGACAGCAGTTGGTG+AGG - chr4_4:12495873-12495892 None:intergenic 50.0%
TCACTTGTCGGTGATGCGAA+TGG + chr4_4:12496025-12496044 Msa0670220:CDS 50.0%
AGGCAGCTTAGCATTAAGGC+CGG + chr4_4:12496118-12496137 Msa0670220:CDS 50.0%
ACACACTGGAGAGTTCTTCC+CGG - chr4_4:12496140-12496159 None:intergenic 50.0%
ACGGGTAAGAAGTGACACAC+TGG - chr4_4:12496154-12496173 None:intergenic 50.0%
AGCGAGCCTTACATGACTGG+CGG + chr4_4:12494456-12494475 Msa0670220:CDS 55.0%
CTGTACCCGCCAGTCATGTA+AGG - chr4_4:12494465-12494484 None:intergenic 55.0%
GGCGGGTACAGACTTGCATA+TGG + chr4_4:12494474-12494493 Msa0670220:CDS 55.0%
AGCGAGCCTTACATGACTGG+CGG + chr4_4:12494456-12494475 Msa0670220:CDS 55.0%
CTGTACCCGCCAGTCATGTA+AGG - chr4_4:12494465-12494484 None:intergenic 55.0%
GGCGGGTACAGACTTGCATA+TGG + chr4_4:12494474-12494493 Msa0670220:CDS 55.0%
AGCGAGCCTTACATGACTGG+CGG + chr4_4:12494456-12494475 Msa0670220:CDS 55.0%
CTGTACCCGCCAGTCATGTA+AGG - chr4_4:12494465-12494484 None:intergenic 55.0%
GGCGGGTACAGACTTGCATA+TGG + chr4_4:12494474-12494493 Msa0670220:CDS 55.0%
! TGGTAGATGGGGGGCCTTTT+GGG - chr4_4:12495463-12495482 None:intergenic 55.0%
! CGTTTGAGGTGGTAGATGGG+GGG - chr4_4:12495472-12495491 None:intergenic 55.0%
GGGGCCTTCTAGATCGTTTG+AGG - chr4_4:12495486-12495505 None:intergenic 55.0%
AGGCCCCCAATCTCCTAACT+TGG + chr4_4:12495499-12495518 Msa0670220:CDS 55.0%
GGCAGCTTAGCATTAAGGCC+GGG + chr4_4:12496119-12496138 Msa0670220:CDS 55.0%
GCGAGCCTTACATGACTGGC+GGG + chr4_4:12494457-12494476 Msa0670220:CDS 60.0%
GCGAGCCTTACATGACTGGC+GGG + chr4_4:12494457-12494476 Msa0670220:CDS 60.0%
GCGAGCCTTACATGACTGGC+GGG + chr4_4:12494457-12494476 Msa0670220:CDS 60.0%
! GTGGTAGATGGGGGGCCTTT+TGG - chr4_4:12495464-12495483 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 12493010 12496222 12493010 ID=Msa0670220;Name=Msa0670220
chr4_4 mRNA 12493010 12496222 12493010 ID=Msa0670220-mRNA-1;Parent=Msa0670220;Name=Msa0670220-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|663
chr4_4 exon 12493010 12493063 12493010 ID=Msa0670220-mRNA-1:exon:3786;Parent=Msa0670220-mRNA-1
chr4_4 exon 12494285 12496222 12494285 ID=Msa0670220-mRNA-1:exon:3787;Parent=Msa0670220-mRNA-1
chr4_4 CDS 12493010 12493063 12493010 ID=Msa0670220-mRNA-1:cds;Parent=Msa0670220-mRNA-1
chr4_4 CDS 12494285 12496222 12494285 ID=Msa0670220-mRNA-1:cds;Parent=Msa0670220-mRNA-1
Gene Sequence

>Msa0670220

ATGGCTACTGCACTAGCACTTCAGTTCCTCACAGTAGTTATTTTCTTCTTTATGATTGGCCAAGACATCAATGCCAGAGACTTGAAGGTAGAGCTGCAAGATCAGGATCAAGTGGACTCGAATGATAAACCATACATTACCCAGTACAGAGGCAGTAAACCAAATTACCCAGCTTCATACACCACCACCAAACACAACAAGCATATATCACATCTCGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCAGATTACACTACAGGGTACAAGACCCATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAAAACCCGGATTACACTACAGGGTACAAGACCCATATCCATGAATCTAATGAAAAGCTAATCGAGCCTTACATGACTGGCGGATACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCCAGTGGACCTTACATAACTGCCTACGGAAGACATGAAGCCAAACAGCTGGATTCCAATGGGCCTTACATTACTCAATATGGTAAACATGAAACCAAGAACCCCGATTACACTACCGGGTACAAGACCCATACCCATGAATCCATTGAACCTTACAACACTGCCTATGGTAAACATGAAGCCAAACAATCAGAATCCAATGGGCCTTACATAACTCAATATGGTAAACATGAATCCAAGAACCCAGATTACACTACCGGGTACAAGACCCATACCCATGCATCCAATGAACCTTACAACACTGCCTATGGTAAACATGAAGCCAAACAATCAGAATCCAATGGGCCTTACATAACTCAATATGGTAAACATGAAGCCAAACAACAAGAATCCAATGAACCTTACACAACTTCCTATGGTAAACAAGAAGCAAATCAACAAAGTTACAATACCGGATATAACACTCATACACAGGAATCTAATGAATTGTACGTATCTGAATATGGTAACCATGAAGCCAAGCAACCAAATTACCTTAACGGGTACATATCTGCTGGAAATGACCCAAAAGGCCCCCCATCTACCACCTCAAACGATCTAGAAGGCCCCCAATCTCCTAACTTGGATCACACAGAAGCTTTCAAGGTAGGATTTTTCAATTTGGATGATCTTTATGTTGGAAATGTAATGACCCTTCAATTTCCCGTCCAAGTGGTTTCTCCTTTTCTCTCAAAAAAAGAGGCTGACTCCATTCCTTTTGCAATGTCACAACTCCCAAGTGTTCTTCAACTCTTCTCAATCCCTGAAGATTCTCCTCAAGCTAAATCCATGATAAATTCACTTGGAGAGTGTGAATCAGAAACCATTACAGGGGAGACAAAGACTTGTGCCAACTCTCTAGAGTCCATGCTCGAATTTGTTGACATGATCATTGGTTCAGACGCCAAACATAGTATTCTCACCACCAACAAACCCTCACCAACTGCTGTCCCTTTACAGAAGTACACCATTTTGGAAGTATCACATGATATTGATGCTCCCAAATGGGTAGCTTGTCATCCACTCTCATATCCATATGCCATTTACTTTTGTCATTACATAGCTACAGGGACTAGAGTGTTCAAGGTCTCACTTGTCGGTGATGCGAATGGAGATAAAATGAAAGCTGTTGGAATTTGCCATTTGGACACATCTGATTGGAACCCAGATCATATTATATTTAGGCAGCTTAGCATTAAGGCCGGGAAGAACTCTCCAGTGTGTCACTTCTTACCCGTGAACCATCTTATGTGGGTTCCAATGCAACCTTCAAAAGCCACCATGTGA
Protein sequence

>Msa0670220

MATALALQFLTVVIFFFMIGQDINARDLKVELQDQDQVDSNDKPYITQYRGSKPNYPASYTTTKHNKHISHLEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHIHESNEKLIEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESSGPYITAYGRHEAKQLDSNGPYITQYGKHETKNPDYTTGYKTHTHESIEPYNTAYGKHEAKQSESNGPYITQYGKHESKNPDYTTGYKTHTHASNEPYNTAYGKHEAKQSESNGPYITQYGKHEAKQQESNEPYTTSYGKQEANQQSYNTGYNTHTQESNELYVSEYGNHEAKQPNYLNGYISAGNDPKGPPSTTSNDLEGPQSPNLDHTEAFKVGFFNLDDLYVGNVMTLQFPVQVVSPFLSKKEADSIPFAMSQLPSVLQLFSIPEDSPQAKSMINSLGECESETITGETKTCANSLESMLEFVDMIIGSDAKHSILTTNKPSPTAVPLQKYTILEVSHDIDAPKWVACHPLSYPYAIYFCHYIATGTRVFKVSLVGDANGDKMKAVGICHLDTSDWNPDHIIFRQLSIKAGKNSPVCHFLPVNHLMWVPMQPSKATM*