AlfalfaGEDB | Msa0036230

M. sativa cultivar zhongmu-4 / Msa0036230

KEGG: -
GO: -
PFAM: PF00139.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0036230	GAU17896.1	39.098	266	96	10	1	215	1	251	5.43e-34	139
Msa0036230	GAU17896.1	75.102	245	58	2	206	450	24	265	1.61e-119	360

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0036230	sp\|Q41160\|LCB3_ROBPS	56.048	248	100	4	209	451	29	272	1.57e-84	263
Msa0036230	sp\|Q41160\|LCB3_ROBPS	41.296	247	89	7	9	206	4	243	2.93e-42	154

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0036230	A0A072VPS3	37.879	264	107	9	1	215	1	256	5.28e-34	138
Msa0036230	A0A072VPS3	73.600	250	61	2	204	450	27	274	2.51e-116	351

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa0036230	0.802380	5.767344e-49	-8.615850e-47
Msa0012820	Msa0036230	0.841571	4.383079e-58	-8.615850e-47
Msa0028280	Msa0036230	-0.817296	3.549640e-52	-8.615850e-47
Msa0036230	Msa0036580	0.850889	1.267771e-60	-8.615850e-47
Msa0036230	Msa0037730	0.801424	9.063863e-49	-8.615850e-47
Msa0036230	Msa0039200	-0.801131	1.040461e-48	-8.615850e-47
Msa0036230	Msa0048950	0.800424	1.450906e-48	-8.615850e-47
Msa0036230	Msa0051100	-0.823405	1.410200e-53	-8.615850e-47
Msa0036230	Msa0055280	-0.806430	8.255988e-50	-8.615850e-47
Msa0036230	Msa0059880	0.809629	1.721483e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0036230	MtrunA17_Chr1g0197791	71.008	238	32	4	1	203	1	236	1.66e-104	311
Msa0036230	MtrunA17_Chr1g0197791	42.802	257	116	9	207	453	30	265	8.44e-46	160
Msa0036230	MtrunA17_Chr3g0087611	65.748	254	34	4	1	203	1	252	6.71e-101	303
Msa0036230	MtrunA17_Chr3g0087611	45.736	258	123	9	207	453	30	281	3.52e-53	179
Msa0036230	MtrunA17_Chr1g0197761	61.600	250	46	6	1	204	1	246	1.62e-94	286
Msa0036230	MtrunA17_Chr1g0197761	40.000	245	129	8	207	443	30	264	1.03e-45	159
Msa0036230	MtrunA17_Chr1g0197801	56.031	257	69	4	203	450	26	247	8.53e-85	260
Msa0036230	MtrunA17_Chr1g0197801	37.118	229	94	6	1	200	1	208	1.68e-30	118
Msa0036230	MtrunA17_Chr3g0100031	52.720	239	109	3	201	439	20	254	1.78e-75	236
Msa0036230	MtrunA17_Chr3g0100031	40.816	245	88	8	6	203	3	237	1.46e-36	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0036230	AT5G65600.1	36.087	230	129	8	212	426	35	261	1.20e-25	110
Msa0036230	AT5G65600.1	30.769	182	93	6	44	205	92	260	7.28e-11	64.7
Msa0036230	AT5G10530.1	35.102	245	135	11	212	440	19	255	2.47e-23	103
Msa0036230	AT5G55830.1	31.674	221	129	8	236	438	51	267	7.25e-17	83.6
Msa0036230	AT5G06740.2	31.250	256	145	12	201	436	25	269	7.57e-17	83.6
Msa0036230	AT5G06740.1	31.250	256	145	12	201	436	13	257	7.94e-17	83.6
Msa0036230	AT4G29050.1	29.386	228	136	8	231	438	39	261	2.05e-16	82.4
Msa0036230	AT4G29050.2	29.386	228	136	8	231	438	53	275	2.23e-16	82.0
Msa0036230	AT3G08870.1	25.475	263	170	7	199	437	20	280	2.64e-15	79.0
Msa0036230	AT3G08870.2	25.475	263	170	7	199	437	41	301	3.08e-15	78.6

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Find 117 sgRNAs with CRISPR-Local

Find 563 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTGCACTTTCTGAGT+TGG	0.481047	1_1:+64701782	None:intergenic
AAAAGTTTGCAATATTGTTT+GGG	0.329321	1_1:+64694369	None:intergenic
AAACCAATCCTTACGGTGTC+AGG	0.427333	1_1:+64694473	None:intergenic
AAACGTGCACTTTCTGAGTT+GGG	0.453097	1_1:+64701783	None:intergenic
AAAGACAGTGAAATGGAATA+GGG	0.472851	1_1:-64694624	Msa0036230:CDS
AAAGTTTGCAATATTGTTTG+GGG	0.709279	1_1:+64694370	None:intergenic
AAATCACGGAACTTCCATGC+AGG	0.555393	1_1:+64695014	None:intergenic
AAATCTATGAGGTACCATAT+TGG	0.393883	1_1:-64701681	Msa0036230:CDS
AACAACACTCAATGTGTTAG+AGG	0.369181	1_1:+64701566	None:intergenic
AACAGAGTTGGCAGGAATAT+TGG	0.331936	1_1:+64694784	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTGGTGAAATTGAAAGAAA+GGG	+	chr1_1:64694464-64694483	None:intergenic	30.0%
ATGCTCAAACGTTAAACAAT+GGG	-	chr1_1:64694533-64694552	Msa0036230:CDS	30.0%
TAAAATGAAAAGTGGATCAG+AGG	+	chr1_1:64694758-64694777	None:intergenic	30.0%
TGGTGTATAATGACATAAAG+GGG	+	chr1_1:64695046-64695065	None:intergenic	30.0%
GATGGTGTATAATGACATAA+AGG	+	chr1_1:64695048-64695067	None:intergenic	30.0%
TACACCATCCATATATGAAT+TGG	-	chr1_1:64695059-64695078	Msa0036230:CDS	30.0%
TTGTCCAATTCATATATGGA+TGG	+	chr1_1:64695066-64695085	None:intergenic	30.0%
CCAATCAACTAGTTAATAAC+AGG	+	chr1_1:64695311-64695330	None:intergenic	30.0%
CCTGTTATTAACTAGTTGAT+TGG	-	chr1_1:64695308-64695327	Msa0036230:intron	30.0%
TCTTACAAATATTTCCATGG+TGG	+	chr1_1:64695520-64695539	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	64694358	64703259	64694358	ID=Msa0036230;Name=Msa0036230
chr1_1	mRNA	64694358	64703259	64694358	ID=Msa0036230-mRNA-1;Parent=Msa0036230;Name=Msa0036230-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|0\|0.25\|4\|0\|453
chr1_1	exon	64703043	64703259	64703043	ID=Msa0036230-mRNA-1:exon:20774;Parent=Msa0036230-mRNA-1
chr1_1	exon	64701674	64701873	64701674	ID=Msa0036230-mRNA-1:exon:20773;Parent=Msa0036230-mRNA-1
chr1_1	exon	64701460	64701649	64701460	ID=Msa0036230-mRNA-1:exon:20772;Parent=Msa0036230-mRNA-1
chr1_1	exon	64694358	64695112	64694358	ID=Msa0036230-mRNA-1:exon:20771;Parent=Msa0036230-mRNA-1
chr1_1	CDS	64703043	64703259	64703043	ID=Msa0036230-mRNA-1:cds;Parent=Msa0036230-mRNA-1
chr1_1	CDS	64701674	64701873	64701674	ID=Msa0036230-mRNA-1:cds;Parent=Msa0036230-mRNA-1
chr1_1	CDS	64701460	64701649	64701460	ID=Msa0036230-mRNA-1:cds;Parent=Msa0036230-mRNA-1
chr1_1	CDS	64694358	64695112	64694358	ID=Msa0036230-mRNA-1:cds;Parent=Msa0036230-mRNA-1

Gene Sequence

>Msa0036230

ATGGCTTTTTATCGCACAAATCTTCCAACTCATGAACTTGTTTTAGGCATAATATACACAACTTTCTTTCTCTTGCTAGCTACAAATATAAACTCAGTACAAGCCCTTTCTTTCAATTTCACCAAGTTCACTCCTAATGCTCTGACATTTCCTTCTGGTATAACCTTCCAAGGGGATGCTCAAACGTTAAACAATGGGGTCATCGCACTGACTAAGCAGACCACCGGAAACGCTCCAACTGATGGATTGATGTTCTTTATTGCACCACAAGACACTGTGATTCCACCCAACTCAGAAAGTGCACGTTTTGGAGTAGTTGATTCAAAAAATGCTTACAATCAATTTGTTGGTGTAGAGTTTGACCTTTATACCAATCCTTATGATCCCAAATCTATGAGGTACCATATTGGAACCAACGTCATGAAATGGGAATGGGTGAGTGGATCATTGAATGAAGTAAGTATAGCCTATGACTCTCCCTCTAACACATTGAGTGTTGTTGTCACTTATGACAATGGTAAATTTTCTACCATTGCTCAAATCGTTGATTTGAAAACTGTACTCCCCAACACGGTTAGGTTTGGTATATCTGGTGCTTCAGTAACTGCAACAGAACTAGTGACCTCACAAAAAACAGTTTCCTTCAACGTTACCAACTTCAGTACTAGTAAATCCAGTTTTTCCCTGCATGGAAGTTCCGTGATTTTATCCAACGGAATCGTGACAATAAACAATCCAGGAGACCCTAAAAATGTTGTAGGTCGAATCCTGCATAAAACACCAGTGCCCATCTGGGATAGTACCACTGGCAACGTTGCTAGCTTCATCACCTCCTTCACATTCAAAGTTGTAGACTACAAAAAAATGACGCCTGCTAGTGGACTTGTCTTCATTCTTACACCACCAGATCGAGCCAATATTCCTGCCAACTCTGTTGGTGGAAACCTTGGAGTTGTTAATGGAAACAGTCCTCACAATCCATTTGTTGGGGTAGAGTTTGATACTAGTTCCACTGGTTCATGGGATCCCAAACACTCACATGTTGGAATTGATGTCAACTCTTTGATTTCATTAAAGACAGTGAAATGGAATAGGGTGAGTGGCGCCTTGGTGAATGTAAAAATTTCCTATGACAATCTCTCTAAGACATTGAATGTTGTTGTATATTACCCAGATGGTACCTTTTCAACTATTGCTCAAGTCGTTGATTTGAAAGCAGTGCTTCCTGACACCGTAAGGATTGGTTTTTCTGCTGCCTCGACAACAGCTGCCCGTCAATTACACCACCTCCATGCATGGTCTTTCAAGTCAAACTTGGACACAACCACCCCAAACAATATTGCAAACTTTTCAGCCATCTGA

Protein sequence

>Msa0036230

MAFYRTNLPTHELVLGIIYTTFFLLLATNINSVQALSFNFTKFTPNALTFPSGITFQGDAQTLNNGVIALTKQTTGNAPTDGLMFFIAPQDTVIPPNSESARFGVVDSKNAYNQFVGVEFDLYTNPYDPKSMRYHIGTNVMKWEWVSGSLNEVSIAYDSPSNTLSVVVTYDNGKFSTIAQIVDLKTVLPNTVRFGISGASVTATELVTSQKTVSFNVTNFSTSKSSFSLHGSSVILSNGIVTINNPGDPKNVVGRILHKTPVPIWDSTTGNVASFITSFTFKVVDYKKMTPASGLVFILTPPDRANIPANSVGGNLGVVNGNSPHNPFVGVEFDTSSTGSWDPKHSHVGIDVNSLISLKTVKWNRVSGALVNVKISYDNLSKTLNVVVYYPDGTFSTIAQVVDLKAVLPDTVRIGFSAASTTAARQLHHLHAWSFKSNLDTTTPNNIANFSAI*