Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112730 | XP_003623947.3 | 79.412 | 68 | 13 | 1 | 1 | 67 | 1 | 68 | 3.20e-27 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112730 | sp|Q8W4Y8|IBB_LENCU | 67.188 | 64 | 15 | 2 | 3 | 66 | 2 | 59 | 1.29e-20 | 83.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112730 | G7L4L7 | 79.412 | 68 | 13 | 1 | 1 | 67 | 1 | 68 | 1.53e-27 | 107 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0028280 | Msa1112730 | -0.811411 | 7.095686e-51 | -8.615850e-47 |
| Msa0030030 | Msa1112730 | -0.818645 | 1.759724e-52 | -8.615850e-47 |
| Msa0036230 | Msa1112730 | 0.854190 | 1.452114e-61 | -8.615850e-47 |
| Msa0036580 | Msa1112730 | 0.824291 | 8.741072e-54 | -8.615850e-47 |
| Msa0039200 | Msa1112730 | -0.809853 | 1.540626e-50 | -8.615850e-47 |
| Msa0048950 | Msa1112730 | 0.819380 | 1.197124e-52 | -8.615850e-47 |
| Msa0051100 | Msa1112730 | -0.810741 | 9.912421e-51 | -8.615850e-47 |
| Msa0059880 | Msa1112730 | 0.814792 | 1.286428e-51 | -8.615850e-47 |
| Msa0072030 | Msa1112730 | 0.803915 | 2.775118e-49 | -8.615850e-47 |
| Msa0077330 | Msa1112730 | -0.812541 | 4.026138e-51 | -8.615850e-47 |
| Msa0084150 | Msa1112730 | 0.806879 | 6.636876e-50 | -8.615850e-47 |
| Msa0084160 | Msa1112730 | 0.800097 | 1.691316e-48 | -8.615850e-47 |
| Msa0086820 | Msa1112730 | -0.813194 | 2.895785e-51 | -8.615850e-47 |
| Msa0101650 | Msa1112730 | -0.804686 | 1.917845e-49 | -8.615850e-47 |
| Msa0605460 | Msa1112730 | 0.811186 | 7.941802e-51 | -8.615850e-47 |
| Msa0623750 | Msa1112730 | 0.830549 | 2.758694e-55 | -8.615850e-47 |
| Msa0640290 | Msa1112730 | 0.808799 | 2.593425e-50 | -8.615850e-47 |
| Msa0645100 | Msa1112730 | 0.813364 | 2.656739e-51 | -8.615850e-47 |
| Msa0646040 | Msa1112730 | 0.810253 | 1.263311e-50 | -8.615850e-47 |
| Msa0667620 | Msa1112730 | 0.806317 | 8.723389e-50 | -8.615850e-47 |
| Msa0667640 | Msa1112730 | 0.813483 | 2.502288e-51 | -8.615850e-47 |
| Msa0670220 | Msa1112730 | 0.801854 | 7.397030e-49 | -8.615850e-47 |
| Msa0670360 | Msa1112730 | 0.803819 | 2.906372e-49 | -8.615850e-47 |
| Msa0678620 | Msa1112730 | 0.812328 | 4.479891e-51 | -8.615850e-47 |
| Msa0727480 | Msa1112730 | 0.808896 | 2.472373e-50 | -8.615850e-47 |
| Msa0768670 | Msa1112730 | 0.801283 | 9.688746e-49 | -8.615850e-47 |
| Msa0782030 | Msa1112730 | -0.805440 | 1.333475e-49 | -8.615850e-47 |
| Msa0796430 | Msa1112730 | 0.816629 | 5.011930e-52 | -8.615850e-47 |
| Msa0824860 | Msa1112730 | 0.803530 | 3.335995e-49 | -8.615850e-47 |
| Msa0834270 | Msa1112730 | 0.821934 | 3.100448e-53 | -8.615850e-47 |
| Msa0133670 | Msa1112730 | -0.810102 | 1.361728e-50 | -8.615850e-47 |
| Msa0133880 | Msa1112730 | -0.807695 | 4.458236e-50 | -8.615850e-47 |
| Msa0139270 | Msa1112730 | 0.808614 | 2.840750e-50 | -8.615850e-47 |
| Msa0139720 | Msa1112730 | 0.817176 | 3.777983e-52 | -8.615850e-47 |
| Msa0150910 | Msa1112730 | 0.812602 | 3.903732e-51 | -8.615850e-47 |
| Msa0163340 | Msa1112730 | 0.809040 | 2.303387e-50 | -8.615850e-47 |
| Msa0176620 | Msa1112730 | 0.815536 | 8.793205e-52 | -8.615850e-47 |
| Msa0184120 | Msa1112730 | -0.807199 | 5.679720e-50 | -8.615850e-47 |
| Msa0197750 | Msa1112730 | 0.839737 | 1.324914e-57 | -8.615850e-47 |
| Msa0203180 | Msa1112730 | -0.802792 | 4.742944e-49 | -8.615850e-47 |
| Msa0219210 | Msa1112730 | 0.807760 | 4.317604e-50 | -8.615850e-47 |
| Msa0393900 | Msa1112730 | 0.821044 | 4.976754e-53 | -8.615850e-47 |
| Msa0404180 | Msa1112730 | -0.807388 | 5.179289e-50 | -8.615850e-47 |
| Msa0414480 | Msa1112730 | 0.808216 | 3.453263e-50 | -8.615850e-47 |
| Msa0423680 | Msa1112730 | 0.812158 | 4.881124e-51 | -8.615850e-47 |
| Msa0456480 | Msa1112730 | -0.816665 | 4.919299e-52 | -8.615850e-47 |
| Msa0469550 | Msa1112730 | 0.815058 | 1.122824e-51 | -8.615850e-47 |
| Msa0470370 | Msa1112730 | 0.811147 | 8.097267e-51 | -8.615850e-47 |
| Msa0475360 | Msa1112730 | -0.803801 | 2.930604e-49 | -8.615850e-47 |
| Msa0985250 | Msa1112730 | 0.801870 | 7.340401e-49 | -8.615850e-47 |
| Msa0985700 | Msa1112730 | 0.827342 | 1.650027e-54 | -8.615850e-47 |
| Msa0996940 | Msa1112730 | 0.800865 | 1.179202e-48 | -8.615850e-47 |
| Msa0996980 | Msa1112730 | 0.802165 | 6.386701e-49 | -8.615850e-47 |
| Msa1006940 | Msa1112730 | 0.831635 | 1.492811e-55 | -8.615850e-47 |
| Msa1021620 | Msa1112730 | 0.810116 | 1.352226e-50 | -8.615850e-47 |
| Msa1061670 | Msa1112730 | 0.803328 | 3.674027e-49 | -8.615850e-47 |
| Msa1076830 | Msa1112730 | 0.803798 | 2.935663e-49 | -8.615850e-47 |
| Msa1079610 | Msa1112730 | -0.827835 | 1.256442e-54 | -8.615850e-47 |
| Msa1087380 | Msa1112730 | 0.801416 | 9.096656e-49 | -8.615850e-47 |
| Msa1100560 | Msa1112730 | 0.840066 | 1.087448e-57 | -8.615850e-47 |
| Msa0266640 | Msa1112730 | 0.813343 | 2.686078e-51 | -8.615850e-47 |
| Msa0266650 | Msa1112730 | 0.819368 | 1.205037e-52 | -8.615850e-47 |
| Msa0277280 | Msa1112730 | 0.828159 | 1.049555e-54 | -8.615850e-47 |
| Msa0278940 | Msa1112730 | 0.810698 | 1.012696e-50 | -8.615850e-47 |
| Msa0298270 | Msa1112730 | 0.811801 | 5.838112e-51 | -8.615850e-47 |
| Msa0356200 | Msa1112730 | 0.807503 | 4.895869e-50 | -8.615850e-47 |
| Msa0356250 | Msa1112730 | 0.823506 | 1.335206e-53 | -8.615850e-47 |
| Msa1109880 | Msa1112730 | 0.804380 | 2.220990e-49 | -8.615850e-47 |
| Msa1109890 | Msa1112730 | 0.828316 | 9.619443e-55 | -8.615850e-47 |
| Msa1112730 | Msa1132560 | 0.816783 | 4.628253e-52 | -8.615850e-47 |
| Msa1112730 | Msa1136060 | 0.824117 | 9.604373e-54 | -8.615850e-47 |
| Msa1112730 | Msa1142860 | 0.809835 | 1.554794e-50 | -8.615850e-47 |
| Msa1112730 | Msa1155630 | 0.838462 | 2.835457e-57 | -8.615850e-47 |
| Msa1112730 | Msa1219780 | 0.805166 | 1.522226e-49 | -8.615850e-47 |
| Msa1112730 | Msa1221170 | 0.819271 | 1.267882e-52 | -8.615850e-47 |
| Msa1112730 | Msa1233650 | 0.852578 | 4.211927e-61 | -8.615850e-47 |
| Msa1112730 | Msa1265500 | 0.833432 | 5.352256e-56 | -8.615850e-47 |
| Msa1112730 | Msa1266710 | 0.808566 | 2.908564e-50 | -8.615850e-47 |
| Msa1112730 | Msa1269050 | 0.807237 | 5.575833e-50 | -8.615850e-47 |
| Msa1112730 | Msa1282210 | -0.802398 | 5.717287e-49 | -8.615850e-47 |
| Msa1112730 | Msa1300610 | 0.813255 | 2.807260e-51 | -8.615850e-47 |
| Msa1112730 | Msa1314050 | 0.800831 | 1.198201e-48 | -8.615850e-47 |
| Msa1112730 | Msa1314710 | 0.802858 | 4.595724e-49 | -8.615850e-47 |
| Msa1112730 | Msa1323120 | 0.810236 | 1.274483e-50 | -8.615850e-47 |
| Msa1112730 | Msa1323160 | 0.830826 | 2.360308e-55 | -8.615850e-47 |
| Msa1112730 | Msa1329740 | 0.809220 | 2.106804e-50 | -8.615850e-47 |
| Msa1112730 | Msa1342400 | -0.801376 | 9.273257e-49 | -8.615850e-47 |
| Msa1112730 | Msa1350490 | 0.840232 | 9.844234e-58 | -8.615850e-47 |
| Msa1112730 | Msa1357490 | 0.820543 | 6.489572e-53 | -8.615850e-47 |
| Msa1112730 | Msa1357720 | -0.808083 | 3.686323e-50 | -8.615850e-47 |
| Msa1112730 | Msa1360380 | -0.807442 | 5.045476e-50 | -8.615850e-47 |
| Msa1112730 | Msa1361190 | -0.803036 | 4.221596e-49 | -8.615850e-47 |
| Msa1112730 | Msa1376070 | 0.808943 | 2.415470e-50 | -8.615850e-47 |
| Msa1112730 | Msa1380490 | 0.838891 | 2.196387e-57 | -8.615850e-47 |
| Msa1112730 | Msa1393440 | 0.839291 | 1.730486e-57 | -8.615850e-47 |
| Msa1112730 | Msa1395690 | 0.812926 | 3.315417e-51 | -8.615850e-47 |
| Msa1112730 | Msa1405470 | -0.806402 | 8.369060e-50 | -8.615850e-47 |
| Msa1112730 | Msa1407210 | 0.802728 | 4.888972e-49 | -8.615850e-47 |
| Msa1112730 | Msa1409600 | 0.818395 | 2.004402e-52 | -8.615850e-47 |
| Msa1112730 | Msa1420020 | 0.815329 | 9.775845e-52 | -8.615850e-47 |
| Msa1112730 | Msa1445900 | 0.805238 | 1.470203e-49 | -8.615850e-47 |
| Msa1112730 | Msa1452200 | -0.808605 | 2.853124e-50 | -8.615850e-47 |
| Msa1112730 | Msa1459610 | 0.812035 | 5.190075e-51 | -8.615850e-47 |
| Msa0515260 | Msa1112730 | 0.813709 | 2.231376e-51 | -8.615850e-47 |
| Msa0532610 | Msa1112730 | 0.812935 | 3.301024e-51 | -8.615850e-47 |
| Msa0533510 | Msa1112730 | 0.814280 | 1.669649e-51 | -8.615850e-47 |
| Msa0533520 | Msa1112730 | 0.819404 | 1.182219e-52 | -8.615850e-47 |
| Msa0543740 | Msa1112730 | 0.801772 | 7.691113e-49 | -8.615850e-47 |
| Msa0548760 | Msa1112730 | 0.801864 | 7.361679e-49 | -8.615850e-47 |
| Msa0548970 | Msa1112730 | 0.801526 | 8.637789e-49 | -8.615850e-47 |
| Msa0568610 | Msa1112730 | -0.805533 | 1.274808e-49 | -8.615850e-47 |
| Msa0589720 | Msa1112730 | 0.817830 | 2.689950e-52 | -8.615850e-47 |
| Msa0589730 | Msa1112730 | 0.804040 | 2.614108e-49 | -8.615850e-47 |
| Msa0591490 | Msa1112730 | 0.808444 | 3.088598e-50 | -8.615850e-47 |
| Msa0599600 | Msa1112730 | 0.824906 | 6.260683e-54 | -8.615850e-47 |
| Msa0883640 | Msa1112730 | 0.824670 | 7.118738e-54 | -8.615850e-47 |
| Msa0896920 | Msa1112730 | 0.812818 | 3.501365e-51 | -8.615850e-47 |
| Msa0902340 | Msa1112730 | 0.804451 | 2.146824e-49 | -8.615850e-47 |
| Msa0910830 | Msa1112730 | 0.822135 | 2.785181e-53 | -8.615850e-47 |
| Msa0913550 | Msa1112730 | 0.801275 | 9.722347e-49 | -8.615850e-47 |
| Msa0950410 | Msa1112730 | -0.816793 | 4.603743e-52 | -8.615850e-47 |
| Msa0960090 | Msa1112730 | 0.825810 | 3.825468e-54 | -8.615850e-47 |
| Msa0971880 | Msa1112730 | 0.841188 | 5.530011e-58 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1112730 | MtrunA17_Chr7g0247571 | 79.412 | 68 | 13 | 1 | 1 | 67 | 39 | 106 | 7.52e-31 | 107 |
| Msa1112730 | MtrunA17_Chr7g0247481 | 77.193 | 57 | 13 | 0 | 10 | 66 | 1 | 57 | 2.19e-26 | 94.7 |
| Msa1112730 | MtrunA17_Chr7g0247471 | 85.455 | 55 | 6 | 1 | 13 | 67 | 14 | 66 | 2.34e-25 | 92.4 |
| Msa1112730 | MtrunA17_Chr7g0247461 | 65.714 | 70 | 17 | 3 | 1 | 64 | 17 | 85 | 1.35e-22 | 86.3 |
| Msa1112730 | MtrunA17_Chr7g0247511 | 61.765 | 68 | 24 | 2 | 1 | 67 | 1 | 67 | 2.50e-19 | 77.4 |
| Msa1112730 | MtrunA17_Chr7g0247531 | 48.718 | 78 | 36 | 2 | 11 | 85 | 1 | 77 | 7.17e-16 | 68.2 |
| Msa1112730 | MtrunA17_Chr7g0247541 | 61.818 | 55 | 16 | 2 | 4 | 58 | 3 | 52 | 6.15e-14 | 63.2 |
| Msa1112730 | MtrunA17_Chr7g0247581 | 58.621 | 58 | 23 | 1 | 6 | 63 | 23 | 79 | 3.42e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGTCACTGTGCTGATAT+TGG | 0.334596 | 7_3:-58662189 | Msa1112730:CDS |
| AATGATGAAGTTAGCTTTGT+TGG | 0.339085 | 7_3:-58662369 | Msa1112730:CDS |
| TGTCTCTTATCACATGCATA+TGG | 0.447909 | 7_3:+58662016 | None:intergenic |
| TTTGAGCTTCACTGCAACCA+TGG | 0.462992 | 7_3:-58662339 | Msa1112730:CDS |
| TGGTTGCAGTGAAGCTCAAA+AGG | 0.467432 | 7_3:+58662342 | None:intergenic |
| AACTTAGTGAGATCCACCTT+TGG | 0.468934 | 7_3:+58662059 | None:intergenic |
| CCACCTTTGGAGTAGCTACA+TGG | 0.481606 | 7_3:+58662072 | None:intergenic |
| GGAGAGGAGTTGAGTGATGA+AGG | 0.494529 | 7_3:+58662292 | None:intergenic |
| ATCACAGCATGCTGTTGTTG+TGG | 0.550411 | 7_3:+58662241 | None:intergenic |
| ATCGTAACTAGCTTCACCAT+TGG | 0.556263 | 7_3:+58662271 | None:intergenic |
| ATCACTCAACTCCTCTCCAA+TGG | 0.592346 | 7_3:-58662287 | Msa1112730:CDS |
| CCATGTAGCTACTCCAAAGG+TGG | 0.604280 | 7_3:-58662072 | Msa1112730:CDS |
| TCTTATCACATGCATATGGT+AGG | 0.607791 | 7_3:+58662020 | None:intergenic |
| GAGGAAAAGATCTTGTGCAA+AGG | 0.628614 | 7_3:+58662136 | None:intergenic |
| AACTAGCTTCACCATTGGAG+AGG | 0.633390 | 7_3:+58662276 | None:intergenic |
| GAACCATGTAGCTACTCCAA+AGG | 0.655168 | 7_3:-58662075 | Msa1112730:CDS |
| TCAGCACAGTGACACTGAGG+AGG | 0.672508 | 7_3:+58662195 | None:intergenic |
| ATCGAAACGAGCATCAACCA+TGG | 0.682629 | 7_3:+58662322 | None:intergenic |
| TCAGTGCAACGACACTGTGG+AGG | 0.684993 | 7_3:+58662117 | None:intergenic |
| TGGTTCATAGCAGAAGTCAG+TGG | 0.693755 | 7_3:+58662092 | None:intergenic |
| ATATCAGCACAGTGACACTG+AGG | 0.763170 | 7_3:+58662192 | None:intergenic |
| GTATCAGTGCAACGACACTG+TGG | 0.776006 | 7_3:+58662114 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGATGAAGTTAGCTTTGT+TGG | - | chr7_3:58662051-58662070 | Msa1112730:CDS | 30.0% |
| ! | ATCGTAACTAGCTTCACCAT+TGG | + | chr7_3:58662152-58662171 | None:intergenic | 40.0% |
| !! | GGAGGATTGATTTTGTGCAA+AGG | + | chr7_3:58662210-58662229 | None:intergenic | 40.0% |
| GAGGAAAAGATCTTGTGCAA+AGG | + | chr7_3:58662287-58662306 | None:intergenic | 40.0% | |
| AACTTAGTGAGATCCACCTT+TGG | + | chr7_3:58662364-58662383 | None:intergenic | 40.0% | |
| TGGTTGCAGTGAAGCTCAAA+AGG | + | chr7_3:58662081-58662100 | None:intergenic | 45.0% | |
| TTTGAGCTTCACTGCAACCA+TGG | - | chr7_3:58662081-58662100 | Msa1112730:CDS | 45.0% | |
| ATCGAAACGAGCATCAACCA+TGG | + | chr7_3:58662101-58662120 | None:intergenic | 45.0% | |
| ATCACTCAACTCCTCTCCAA+TGG | - | chr7_3:58662133-58662152 | Msa1112730:CDS | 45.0% | |
| ! | AACTAGCTTCACCATTGGAG+AGG | + | chr7_3:58662147-58662166 | None:intergenic | 45.0% |
| ATCACAGCATGCTGTTGTTG+TGG | + | chr7_3:58662182-58662201 | None:intergenic | 45.0% | |
| ATATCAGCACAGTGACACTG+AGG | + | chr7_3:58662231-58662250 | None:intergenic | 45.0% | |
| ! | CAGTGTCACTGTGCTGATAT+TGG | - | chr7_3:58662231-58662250 | Msa1112730:CDS | 45.0% |
| TGGTTCATAGCAGAAGTCAG+TGG | + | chr7_3:58662331-58662350 | None:intergenic | 45.0% | |
| GAACCATGTAGCTACTCCAA+AGG | - | chr7_3:58662345-58662364 | Msa1112730:CDS | 45.0% | |
| GGAGAGGAGTTGAGTGATGA+AGG | + | chr7_3:58662131-58662150 | None:intergenic | 50.0% | |
| GTATCAGTGCAACGACACTG+TGG | + | chr7_3:58662309-58662328 | None:intergenic | 50.0% | |
| !! | CCACCTTTGGAGTAGCTACA+TGG | + | chr7_3:58662351-58662370 | None:intergenic | 50.0% |
| CCATGTAGCTACTCCAAAGG+TGG | - | chr7_3:58662348-58662367 | Msa1112730:CDS | 50.0% | |
| TCAGCACAGTGACACTGAGG+AGG | + | chr7_3:58662228-58662247 | None:intergenic | 55.0% | |
| TCAGTGCAACGACACTGTGG+AGG | + | chr7_3:58662306-58662325 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 58662025 | 58662417 | 58662025 | ID=Msa1112730;Name=Msa1112730 |
| chr7_3 | mRNA | 58662025 | 58662417 | 58662025 | ID=Msa1112730-mRNA-1;Parent=Msa1112730;Name=Msa1112730-mRNA-1;_AED=0.52;_eAED=0.66;_QI=0|-1|0|1|-1|1|1|0|130 |
| chr7_3 | exon | 58662025 | 58662417 | 58662025 | ID=Msa1112730-mRNA-1:exon:12923;Parent=Msa1112730-mRNA-1 |
| chr7_3 | CDS | 58662025 | 58662417 | 58662025 | ID=Msa1112730-mRNA-1:cds;Parent=Msa1112730-mRNA-1 |
| Gene Sequence |
| Protein sequence |
