AlfalfaGEDB | Msa0605460

M. sativa cultivar zhongmu-4 / Msa0605460

KEGG: -
GO: -
PFAM: PF03181.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0605460	XP_013456397.1	67.922	770	142	8	1	719	1	716	0.0	991

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0605460	sp\|O65009\|BNM2A_BRANA	38.288	222	129	3	496	710	60	280	1.14e-45	167

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012780	Msa0605460	0.810048	1.398660e-50	-8.615850e-47
Msa0012820	Msa0605460	0.847323	1.243843e-59	-8.615850e-47
Msa0036230	Msa0605460	0.844243	8.528428e-59	-8.615850e-47
Msa0036580	Msa0605460	0.853499	2.294863e-61	-8.615850e-47
Msa0048950	Msa0605460	0.836880	7.217896e-57	-8.615850e-47
Msa0055280	Msa0605460	-0.808811	2.578425e-50	-8.615850e-47
Msa0059880	Msa0605460	0.841649	4.179477e-58	-8.615850e-47
Msa0061600	Msa0605460	0.880541	5.041773e-70	-8.615850e-47
Msa0084160	Msa0605460	0.850314	1.839376e-60	-8.615850e-47
Msa0101060	Msa0605460	-0.802925	4.451395e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0605460	MtrunA17_Chr4g0034691	67.922	770	142	8	1	719	1	716	0.0	991
Msa0605460	MtrunA17_Chr4g0034721	72.222	594	101	10	145	719	36	584	0.0	805
Msa0605460	MtrunA17_Chr4g0034721	84.146	328	32	3	3	323	4	318	0.0	545
Msa0605460	MtrunA17_Chr4g0034731	66.355	428	107	6	307	712	21	433	0.0	560
Msa0605460	MtrunA17_Chr4g0034731	49.356	233	54	6	1	217	1	185	1.99e-44	165
Msa0605460	MtrunA17_Chr4g0034731	58.784	148	38	3	234	370	42	177	1.72e-38	148
Msa0605460	MtrunA17_Chr4g0034731	50.262	191	37	8	171	349	41	185	1.82e-36	142
Msa0605460	MtrunA17_Chr4g0034731	51.099	182	40	8	133	302	41	185	4.67e-36	141
Msa0605460	MtrunA17_Chr4g0034451	65.116	301	84	4	421	717	249	532	1.98e-137	415
Msa0605460	MtrunA17_Chr3g0144391	51.877	293	128	6	425	714	226	508	7.84e-95	303

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0605460	AT1G49320.1	35.586	222	135	3	496	710	58	278	6.56e-45	162
Msa0605460	AT5G25610.1	35.160	219	132	6	496	709	176	389	6.37e-36	140
Msa0605460	AT1G60390.1	29.897	194	124	6	520	709	435	620	9.60e-24	107
Msa0605460	AT1G70370.2	31.282	195	122	7	520	710	437	623	7.33e-23	104
Msa0605460	AT1G70370.1	31.282	195	122	7	520	710	437	623	7.33e-23	104
Msa0605460	AT1G23760.1	28.351	194	129	6	520	710	433	619	2.85e-20	95.9

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Find 232 sgRNAs with CRISPR-Local

Find 307 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGAAACCAAGAACCC+AGG	0.692067	4_2:-51382537	Msa0605460:CDS
AAACCATACATTACCCAGTA+CGG	0.577354	4_2:-51383416	Msa0605460:CDS
AAAGGAGAAACCACTTGGAC+GGG	0.559421	4_2:+51382006	None:intergenic
AAAGGGACAGCCGTTGGTGA+GGG	0.570768	4_2:+51381736	None:intergenic
AAATCCATGATAAATTCACT+TGG	0.603290	4_2:-51381886	Msa0605460:CDS
AAGAACCCAGATTACACTAC+CGG	0.556042	4_2:-51382666	Msa0605460:CDS
AAGAACCCAGGTTACACTAC+CGG	0.552435	4_2:-51382525	Msa0605460:CDS
AAGAACCCGGATTACACTAC+AGG	0.510637	4_2:-51382807	Msa0605460:CDS
AAGAACCCGGATTACACTAC+AGG	0.510637	4_2:-51382921	Msa0605460:CDS
AAGAACCCGGATTACACTAC+AGG	0.510637	4_2:-51383035	Msa0605460:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTGCACATAGTGATAGTT+GGG	-	chr4_2:51381466-51381485	Msa0605460:CDS	30.0%
ATTAGATACGGAGAAAAAAG+AGG	-	chr4_2:51381544-51381563	Msa0605460:CDS	30.0%
TTAGATACGGAGAAAAAAGA+GGG	-	chr4_2:51381545-51381564	Msa0605460:CDS	30.0%
CCTATAGAAATTGCATTGTT+TGG	+	chr4_2:51381707-51381726	None:intergenic	30.0%
CCAAACAATGCAATTTCTAT+AGG	-	chr4_2:51381704-51381723	Msa0605460:CDS	30.0%
TTGAGTCACACAATTAAATG+TGG	+	chr4_2:51381922-51381941	None:intergenic	30.0%
TCTGATTAAAAGTGACTTGA+AGG	+	chr4_2:51382649-51382668	None:intergenic	30.0%
TTCATTAGATTCATGGGTAT+GGG	+	chr4_2:51383554-51383573	None:intergenic	30.0%
TTTCATTAGATTCATGGGTA+TGG	+	chr4_2:51383555-51383574	None:intergenic	30.0%
TTCATTAGATTCATGGGAAT+GGG	+	chr4_2:51383782-51383801	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	51381405	51385400	51381405	ID=Msa0605460;Name=Msa0605460
chr4_2	mRNA	51381405	51385400	51381405	ID=Msa0605460-mRNA-1;Parent=Msa0605460;Name=Msa0605460-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|2\|0\|719
chr4_2	exon	51385347	51385400	51385347	ID=Msa0605460-mRNA-1:exon:12315;Parent=Msa0605460-mRNA-1
chr4_2	exon	51381405	51383510	51381405	ID=Msa0605460-mRNA-1:exon:12314;Parent=Msa0605460-mRNA-1
chr4_2	CDS	51385347	51385400	51385347	ID=Msa0605460-mRNA-1:cds;Parent=Msa0605460-mRNA-1
chr4_2	CDS	51381405	51383510	51381405	ID=Msa0605460-mRNA-1:cds;Parent=Msa0605460-mRNA-1

Gene Sequence

>Msa0605460

ATGGCTACTGCACTAGCACTTCAGTTCCTCACAGTAGTTCTTTTCTTCTTTATGATTGGCCAAGAAATCAATGCCAGAGACTTGAAGGTAGAGCTGCAAGATCAGGATCAAGTGGACTCGAATGATAAACCATACATTACCCAGTACGGAGGCAGTAAACCAAATTACCCAGCTTCATACACCACCATCAAACACAACAAGCATATATCACATCTCGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGATACATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGATACAGACTTGCATACGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATTCCCATGAATCTAATGAAAAGCTAAGTGAGCCTTACATGACTGGCGGGTACAGACTTGCATACGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCAATGAATCCAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCCAGTGGACCTTACATAACTGCCTACGGAAGACATGAAGCCAAACAACTGGATTCCAATGGGCCTTACATTACTCAATATGGTAAACATGAAACCAAGAACCCAGATTACACTACCGGGTACAAGACCCATACCCATGAATCCATTGAACCTTACAACACTGCCTATGCTAAACATGAAGCCAAACAATCAGAATCCAATGGGCCTTACATAACTCAATATCGTAAACATGAAACCAAGAACCCAGGTTACACTACCGGGTACAAGACCCATACCCATGCATCCAATGAACCTTACAACACTGCCTATGGTAAGCATGAAGCCAAACAATCAGAATCCAATGGACCTTACATAACTCAATATGGTAAACATGAAGCCAAACAATCAGAATCCAATGGGCCTTACATAACTCAATATGGTAAACATGAAGCCAAACAACAAGAATCCAATGGACCTTACACAACTTCCTATGGTAAACAAGAAGCAAAGCAACAAAGTTACAATACCGGATATAACACTCATACCCAGGAATCTAATGAATTGTACGTATCTGCATATGGTAACCATGAAGCCAAGCAACCAAATTACCTTAACGGGTACATATCTGCTGGAAATGACCCAAAAGGCCCCCCATCTACCACCTCAAACGATCTAGAAGGCCCCCAATCTCCTAACTTGGACCACACAGAAGCTTTCAAGGTAGGATTTTTCAATTTGGATGATCTTTATGCTGAAAATGTAATGACCCTTCAATTTCCCGTCCAAGTGGTTTCTCCTTTCCTCTCTAAAAAAGAGGCTGACTCCATTCCTCTTGCAATGTCACAACTCCCAAGTGTTTTTCAACTCTTCTCAATCCCTGAAGATTCTCCTCAAGCTAAATCCATGATAAATTCACTTGGAGAGTGTGAATCGGAAACCATTACAGGGGAGACAAAGACTTGTGCCAACTCTCTAGAGTCCATGCTCGAATTTGTTGACATGATCATTGGTTCAGACGCCAAACATAGTATTCTCACCACCAGCAAACCCTCACCAACGGCTGTCCCTTTACAAAAGTACACCATTTTGGAAGTATCACATGATATTGATGCTCCCAAATGGGTAGCTTGTCATCCACTCTCATATCCATATGCCATTTACTTTTGTCATTACATAGCTACAGGGACTAGAGTGTTCAAGGTCTCACTTGTCGGTGATGCGAATGGAGATAAAATGGAAGCTGTTGGAATTTGCCATTTGGACACATCTGATTGGAACCCAGATCATATTATATTTAGGCAGCTTGGCATTAAGGCCGGGAAGAACTCTCCAGTGTGTCACTTCTTACCTGTGAACCATCTTATGTGGGTTCCAATGCAACCTTCAAAAGCCTCCATGTGA

Protein sequence

>Msa0605460

MATALALQFLTVVLFFFMIGQEINARDLKVELQDQDQVDSNDKPYITQYGGSKPNYPASYTTIKHNKHISHLEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKIHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHSHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTNESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESSGPYITAYGRHEAKQLDSNGPYITQYGKHETKNPDYTTGYKTHTHESIEPYNTAYAKHEAKQSESNGPYITQYRKHETKNPGYTTGYKTHTHASNEPYNTAYGKHEAKQSESNGPYITQYGKHEAKQSESNGPYITQYGKHEAKQQESNGPYTTSYGKQEAKQQSYNTGYNTHTQESNELYVSAYGNHEAKQPNYLNGYISAGNDPKGPPSTTSNDLEGPQSPNLDHTEAFKVGFFNLDDLYAENVMTLQFPVQVVSPFLSKKEADSIPLAMSQLPSVFQLFSIPEDSPQAKSMINSLGECESETITGETKTCANSLESMLEFVDMIIGSDAKHSILTTSKPSPTAVPLQKYTILEVSHDIDAPKWVACHPLSYPYAIYFCHYIATGTRVFKVSLVGDANGDKMEAVGICHLDTSDWNPDHIIFRQLGIKAGKNSPVCHFLPVNHLMWVPMQPSKASM*