AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0764700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0764700 MtrunA17_Chr5g0415221 97.778 90 0 2 20 107 35 124 1.35e-41 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0764700 AT1G69970.2 34.043 94 60 2 15 107 10 102 2.48e-12 58.5

Find 56 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAGGTTCTTAGAATTTC+TGG 0.050081 5_2:+25080826 Msa0764700:three_prime_UTR
TTCAGGAGAGCTGGAAATTC+TGG 0.279686 5_2:+25080700 Msa0764700:intron
AGAGAAGGAAGAGTAGTATA+AGG 0.292356 5_2:-25078515 None:intergenic
TTAAGGAAATTGATTTGTTA+AGG 0.305219 5_2:+25080874 Msa0764700:three_prime_UTR
AGGCAACACTTTGCTTTATA+CGG 0.311252 5_2:-25078358 None:intergenic
TCTATATGTCTTATAGTATA+GGG 0.318965 5_2:+25078609 Msa0764700:five_prime_UTR
CCAGAAATTCTAAGAACCTT+AGG 0.344623 5_2:-25080826 None:intergenic
TGCTTTATACGGAGCAATAT+AGG 0.357132 5_2:-25078347 None:intergenic
TTCTATATGTCTTATAGTAT+AGG 0.362452 5_2:+25078608 Msa0764700:five_prime_UTR
GAAAACAAGAGAGAAAATAG+AGG 0.367300 5_2:-25078539 None:intergenic
TAAAGGATAAGGAGTTCTTT+TGG 0.375272 5_2:+25078270 None:intergenic
TTTATGTCATAGGACTATAT+AGG 0.386534 5_2:-25078650 None:intergenic
TAAACAAACCTGTTGTGAAT+AGG 0.400300 5_2:-25078973 None:intergenic
TAAGGAAATTGATTTGTTAA+GGG 0.406164 5_2:+25080875 Msa0764700:three_prime_UTR
CAAAGGGCTAATCACACTTA+AGG 0.424359 5_2:+25080857 Msa0764700:three_prime_UTR
TTTAGAGTACTTGTAGTGGT+GGG 0.429193 5_2:+25078754 Msa0764700:CDS
TTAGTTAGTGGTGGAGGAAC+AGG 0.433994 5_2:+25078805 Msa0764700:CDS
TTTAGTAGCTTAGTTAGTGG+TGG 0.443725 5_2:+25078796 Msa0764700:CDS
GTGAACTTATATTGTGAAGA+TGG 0.444473 5_2:+25081021 Msa0764700:three_prime_UTR
CTTTAGAGTACTTGTAGTGG+TGG 0.451220 5_2:+25078753 Msa0764700:CDS
TGTCAACCATGATGCACAAT+TGG 0.454537 5_2:+25078906 Msa0764700:CDS
GACCAGATCCTATTCACAAC+AGG 0.455835 5_2:+25078965 Msa0764700:CDS
CATGATGAACAAGTTGTTGT+TGG 0.456308 5_2:+25078865 Msa0764700:CDS
ATTTCTGGCTTGTATGCAAA+GGG 0.456565 5_2:+25080841 Msa0764700:three_prime_UTR
CAGAAATTCTAAGAACCTTA+GGG 0.467727 5_2:-25080825 None:intergenic
TGAACTTATATTGTGAAGAT+GGG 0.468741 5_2:+25081022 Msa0764700:three_prime_UTR
AGAGAAAATAGAGGGAGAGA+AGG 0.476996 5_2:-25078530 None:intergenic
TGAATAGGATCTGGTCCATT+GGG 0.478116 5_2:-25078958 None:intergenic
TGGAGAGTCACAAATTTCAA+TGG 0.493022 5_2:+25081057 Msa0764700:three_prime_UTR
GTGGCCTTAAAAGGGAAAAG+AGG 0.499391 5_2:+25080907 Msa0764700:three_prime_UTR
GGAAATTCTGGTAGACCTCC+TGG 0.500969 5_2:+25080712 Msa0764700:CDS
AGTAGCTTAGTTAGTGGTGG+AGG 0.503163 5_2:+25078799 Msa0764700:CDS
TTAGAGTACTTGTAGTGGTG+GGG 0.504583 5_2:+25078755 Msa0764700:CDS
AAGATGGGGTTAGACAGAAA+TGG 0.513157 5_2:+25081037 Msa0764700:three_prime_UTR
AACCTGTTGTGAATAGGATC+TGG 0.513984 5_2:-25078967 None:intergenic
GAAGGAAGAGTAGTATAAGG+AGG 0.541045 5_2:-25078512 None:intergenic
TGATTTCATTTACATCCCTA+AGG 0.541887 5_2:+25080810 Msa0764700:three_prime_UTR
CTACGAAGGATTAAGGGGTG+TGG 0.547157 5_2:-25078463 None:intergenic
AATTTCTGGCTTGTATGCAA+AGG 0.547193 5_2:+25080840 Msa0764700:three_prime_UTR
AAAACAAGAGAGAAAATAGA+GGG 0.547310 5_2:-25078538 None:intergenic
TTTCTTTAGAGTACTTGTAG+TGG 0.554539 5_2:+25078750 Msa0764700:CDS
GTGAATAGGATCTGGTCCAT+TGG 0.561168 5_2:-25078959 None:intergenic
GAAGGATTAAGGGGTGTGGT+TGG 0.569824 5_2:-25078459 None:intergenic
TGAAGTCCAATTGTGCATCA+TGG 0.582511 5_2:-25078912 None:intergenic
TCAGTTGACTAAGTTTGACC+AGG 0.583639 5_2:-25080730 None:intergenic
TAGGACTATATGTTTGAGGG+GGG 0.587610 5_2:+25081194 Msa0764700:three_prime_UTR
ATACTTTCTCGAACTAAGAG+AGG 0.589250 5_2:-25078407 None:intergenic
TTAGGACTATATGTTTGAGG+GGG 0.589882 5_2:+25081193 Msa0764700:three_prime_UTR
AGTTGTTGTTGGTAAAGACA+AGG 0.593670 5_2:+25078876 Msa0764700:CDS
TTTAGGACTATATGTTTGAG+GGG 0.612589 5_2:+25081192 Msa0764700:three_prime_UTR
GACAAACAACTACAAGACAT+TGG 0.623172 5_2:+25078827 Msa0764700:CDS
TACTTTCTCGAACTAAGAGA+GGG 0.624715 5_2:-25078406 None:intergenic
GTTGTTGTTGGTAAAGACAA+GGG 0.652393 5_2:+25078877 Msa0764700:CDS
AGTAAAAGAAGAGTTCCCAA+TGG 0.653384 5_2:+25078943 Msa0764700:CDS
GAACTTATATTGTGAAGATG+GGG 0.662145 5_2:+25081023 Msa0764700:three_prime_UTR
GTTGACTAAGTTTGACCAGG+AGG 0.677258 5_2:-25080727 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTATTTTTATGTCAT+AGG - chr5_2:25078663-25078682 None:intergenic 10.0%
!! ATTAATTAAACATGTAAATT+AGG - chr5_2:25079166-25079185 None:intergenic 10.0%
!! AGAAAGTAAATAAATATTAA+AGG + chr5_2:25079345-25079364 Msa0764700:intron 10.0%
!!! TTTTGGATTTTTTTTTTTTT+TGG + chr5_2:25079738-25079757 Msa0764700:intron 10.0%
!! TTTGAAGATATTTAATATAT+AGG - chr5_2:25080366-25080385 None:intergenic 10.0%
!! ATTATAGATGAATAATATAT+TGG - chr5_2:25080621-25080640 None:intergenic 10.0%
!! ATATAGAAGAAGATAAAAAA+AGG - chr5_2:25078596-25078615 None:intergenic 15.0%
!! TTATACAACATTTAGAAAAT+AGG + chr5_2:25079238-25079257 Msa0764700:intron 15.0%
!!! TTTATTTGTTAGTCATTATT+CGG - chr5_2:25079324-25079343 None:intergenic 15.0%
!!! CTTTTTTTTTTTTTTTTGAG+TGG - chr5_2:25079602-25079621 None:intergenic 15.0%
!! AAAAAAAGAGATAAAAACTA+AGG + chr5_2:25079614-25079633 Msa0764700:intron 15.0%
!!! GAATTTTGAAATAGATATTT+AGG - chr5_2:25080240-25080259 None:intergenic 15.0%
!! TGACAAATTTATTTAATGTA+AGG - chr5_2:25080272-25080291 None:intergenic 15.0%
!!! TTGAATTTTTTTTACTACGA+AGG - chr5_2:25078480-25078499 None:intergenic 20.0%
!! TTCTATATGTCTTATAGTAT+AGG + chr5_2:25078608-25078627 Msa0764700:five_prime_UTR 20.0%
!! TCTATATGTCTTATAGTATA+GGG + chr5_2:25078609-25078628 Msa0764700:five_prime_UTR 20.0%
!!! AATCTAGTTTGATTAGATTA+GGG + chr5_2:25079461-25079480 Msa0764700:intron 20.0%
!!! TAACTTTTGATATTATGCTA+AGG + chr5_2:25079564-25079583 Msa0764700:intron 20.0%
!!! AACTTTTGATATTATGCTAA+GGG + chr5_2:25079565-25079584 Msa0764700:intron 20.0%
!!! ACGTATTAAACATTAGTTTT+TGG + chr5_2:25079721-25079740 Msa0764700:intron 20.0%
!! TATCTGTTAACCTATTATAA+AGG - chr5_2:25079995-25080014 None:intergenic 20.0%
!! AAAAGAAAGACAACAAAAAA+AGG - chr5_2:25080138-25080157 None:intergenic 20.0%
!! TGAAATAGATATTTAGGAAA+TGG - chr5_2:25080234-25080253 None:intergenic 20.0%
!! CACAAATTATTAACAAATTC+AGG + chr5_2:25080310-25080329 Msa0764700:intron 20.0%
!! TTAAATATCTTCAAATGCTT+TGG + chr5_2:25080371-25080390 Msa0764700:intron 20.0%
!! TAAATATCTTCAAATGCTTT+GGG + chr5_2:25080372-25080391 Msa0764700:intron 20.0%
!!! TCATTTGGTAACATTTTTTT+TGG + chr5_2:25080668-25080687 Msa0764700:intron 20.0%
!!! TTTTTTGGTAACATTTTTTC+AGG + chr5_2:25080683-25080702 Msa0764700:intron 20.0%
!!! TTAAGGAAATTGATTTGTTA+AGG + chr5_2:25080874-25080893 Msa0764700:three_prime_UTR 20.0%
!!! TAAGGAAATTGATTTGTTAA+GGG + chr5_2:25080875-25080894 Msa0764700:three_prime_UTR 20.0%
!!! CTTTTGAACTTATTTTCTTT+GGG - chr5_2:25080947-25080966 None:intergenic 20.0%
!!! TCTTTTGAACTTATTTTCTT+TGG - chr5_2:25080948-25080967 None:intergenic 20.0%
!!! GGTTTTCTCAATATAAAATT+TGG + chr5_2:25081237-25081256 Msa0764700:three_prime_UTR 20.0%
!! TTTTTACTACGAAGGATTAA+GGG - chr5_2:25078472-25078491 None:intergenic 25.0%
!!! TTTTTTACTACGAAGGATTA+AGG - chr5_2:25078473-25078492 None:intergenic 25.0%
! AAAACAAGAGAGAAAATAGA+GGG - chr5_2:25078541-25078560 None:intergenic 25.0%
! TTTATGTCATAGGACTATAT+AGG - chr5_2:25078653-25078672 None:intergenic 25.0%
!!! AAGAGAAAAAGTATTGCATA+TGG + chr5_2:25078684-25078703 Msa0764700:exon 25.0%
!!! GTAGTAGTTATTTCTTTTCA+AGG + chr5_2:25078725-25078744 Msa0764700:CDS 25.0%
!!! TTCAATTTTTGCCACTTATT+AGG + chr5_2:25079048-25079067 Msa0764700:intron 25.0%
! CATTTAGAAAATAGGATATG+AGG + chr5_2:25079246-25079265 Msa0764700:intron 25.0%
!! GTTTAAATCGGTTCAATTAA+AGG + chr5_2:25079435-25079454 Msa0764700:intron 25.0%
!! CAATCTAGTTTGATTAGATT+AGG + chr5_2:25079460-25079479 Msa0764700:intron 25.0%
! AGGATATAAGTGAAAAGAAA+AGG + chr5_2:25079634-25079653 Msa0764700:intron 25.0%
! ACTAATGTTTAATACGTGTT+AGG - chr5_2:25079718-25079737 None:intergenic 25.0%
! AAGAAGATCGAATCTAATTA+AGG - chr5_2:25079786-25079805 None:intergenic 25.0%
!!! AGATTTTAAAGTCAGAGTTA+AGG - chr5_2:25079854-25079873 None:intergenic 25.0%
! AAATCTTTATCCTGCTATAT+TGG + chr5_2:25079868-25079887 Msa0764700:intron 25.0%
!!! TAGTTAGCTTTCTTTCTTTT+TGG - chr5_2:25079902-25079921 None:intergenic 25.0%
!! CATTGATAGTCCTTTATAAT+AGG + chr5_2:25079982-25080001 Msa0764700:intron 25.0%
!!! CTTCAATTGTTTTTAGTTCA+TGG + chr5_2:25080339-25080358 Msa0764700:intron 25.0%
! AAATATCTTCAAATGCTTTG+GGG + chr5_2:25080373-25080392 Msa0764700:intron 25.0%
!! AGCTCACAAAATTTTAAATG+TGG + chr5_2:25080504-25080523 Msa0764700:intron 25.0%
!! AAATTTTAAATGTGGAGAAG+TGG + chr5_2:25080512-25080531 Msa0764700:intron 25.0%
!! AATTTTAAATGTGGAGAAGT+GGG + chr5_2:25080513-25080532 Msa0764700:intron 25.0%
!! ATTCACTTTAAGAGATCATT+TGG + chr5_2:25080653-25080672 Msa0764700:intron 25.0%
! TGAACTTATATTGTGAAGAT+GGG + chr5_2:25081022-25081041 Msa0764700:three_prime_UTR 25.0%
! AGTCCTAAAAAATATGCATA+TGG - chr5_2:25081181-25081200 None:intergenic 25.0%
!!! TTTTTAGGACTATATGTTTG+AGG + chr5_2:25081190-25081209 Msa0764700:three_prime_UTR 25.0%
!!! TTTTAGGACTATATGTTTGA+GGG + chr5_2:25081191-25081210 Msa0764700:three_prime_UTR 25.0%
! GAAAAAATATATCTTGGCTT+TGG + chr5_2:25081216-25081235 Msa0764700:three_prime_UTR 25.0%
! ATTTTCATGCAATGCAACTA+CGG - chr5_2:25078300-25078319 None:intergenic 30.0%
! TTTTACTACGAAGGATTAAG+GGG - chr5_2:25078471-25078490 None:intergenic 30.0%
GAAAACAAGAGAGAAAATAG+AGG - chr5_2:25078542-25078561 None:intergenic 30.0%
!! TTTCTTTAGAGTACTTGTAG+TGG + chr5_2:25078750-25078769 Msa0764700:CDS 30.0%
!!! GTTTTTAGTAGCTTAGTTAG+TGG + chr5_2:25078793-25078812 Msa0764700:CDS 30.0%
TAAACAAACCTGTTGTGAAT+AGG - chr5_2:25078976-25078995 None:intergenic 30.0%
AGCAATGTATACCTAATAAG+TGG - chr5_2:25079062-25079081 None:intergenic 30.0%
! ACTTGTGTTTTACTTTGCTA+TGG + chr5_2:25079133-25079152 Msa0764700:intron 30.0%
TATGTGACACTCTATATTCT+AGG + chr5_2:25079185-25079204 Msa0764700:intron 30.0%
CTATATTCTAGGTGCAATAT+AGG + chr5_2:25079196-25079215 Msa0764700:intron 30.0%
TAAACTGATTGAACCACAAT+TGG - chr5_2:25079420-25079439 None:intergenic 30.0%
GTGGTTCAATCAGTTTAAAT+CGG + chr5_2:25079423-25079442 Msa0764700:intron 30.0%
GTTAAAAAATCAAACCGAAG+AGG - chr5_2:25079548-25079567 None:intergenic 30.0%
TGTTAGGAAAGGAACATAAT+TGG - chr5_2:25079702-25079721 None:intergenic 30.0%
TGTTTAATACGTGTTAGGAA+AGG - chr5_2:25079713-25079732 None:intergenic 30.0%
GGTATGTGATAAGAATAGAA+AGG - chr5_2:25079765-25079784 None:intergenic 30.0%
AGGACTATCAATGTTAAAAG+AGG - chr5_2:25079975-25079994 None:intergenic 30.0%
GCTTATGAGTAATTAAGAAG+TGG - chr5_2:25080066-25080085 None:intergenic 30.0%
TTCAGAGAGAAAAAATGACA+TGG - chr5_2:25080101-25080120 None:intergenic 30.0%
! ATATATTTTGTCAAGTACCC+TGG + chr5_2:25080474-25080493 Msa0764700:intron 30.0%
! ATTTTAAATGTGGAGAAGTG+GGG + chr5_2:25080514-25080533 Msa0764700:intron 30.0%
ACATTGCAATAATATATGCG+GGG - chr5_2:25080562-25080581 None:intergenic 30.0%
GACATTGCAATAATATATGC+GGG - chr5_2:25080563-25080582 None:intergenic 30.0%
GGACATTGCAATAATATATG+CGG - chr5_2:25080564-25080583 None:intergenic 30.0%
ATGAATAATATATTGGTCCC+TGG - chr5_2:25080614-25080633 None:intergenic 30.0%
ATGCAGAAGTTGACAAAAAA+AGG + chr5_2:25080776-25080795 Msa0764700:three_prime_UTR 30.0%
TGCAGAAGTTGACAAAAAAA+GGG + chr5_2:25080777-25080796 Msa0764700:three_prime_UTR 30.0%
TGATTTCATTTACATCCCTA+AGG + chr5_2:25080810-25080829 Msa0764700:three_prime_UTR 30.0%
! CAGAAATTCTAAGAACCTTA+GGG - chr5_2:25080828-25080847 None:intergenic 30.0%
GTGAACTTATATTGTGAAGA+TGG + chr5_2:25081021-25081040 Msa0764700:three_prime_UTR 30.0%
GAACTTATATTGTGAAGATG+GGG + chr5_2:25081023-25081042 Msa0764700:three_prime_UTR 30.0%
!!! CTGCCATATGCATATTTTTT+AGG + chr5_2:25081175-25081194 Msa0764700:three_prime_UTR 30.0%
! TTTAGGACTATATGTTTGAG+GGG + chr5_2:25081192-25081211 Msa0764700:three_prime_UTR 30.0%
TGCTTTATACGGAGCAATAT+AGG - chr5_2:25078350-25078369 None:intergenic 35.0%
AGGCAACACTTTGCTTTATA+CGG - chr5_2:25078361-25078380 None:intergenic 35.0%
!! AGAGATTTTTAAGCGTGACA+TGG - chr5_2:25078384-25078403 None:intergenic 35.0%
TACTTTCTCGAACTAAGAGA+GGG - chr5_2:25078409-25078428 None:intergenic 35.0%
ATACTTTCTCGAACTAAGAG+AGG - chr5_2:25078410-25078429 None:intergenic 35.0%
!! GGTTTTCTTTATAGGTTGGA+GGG - chr5_2:25078441-25078460 None:intergenic 35.0%
!! TGGTTTTCTTTATAGGTTGG+AGG - chr5_2:25078442-25078461 None:intergenic 35.0%
!!! GGTTGGTTTTCTTTATAGGT+TGG - chr5_2:25078445-25078464 None:intergenic 35.0%
!!! GTGTGGTTGGTTTTCTTTAT+AGG - chr5_2:25078449-25078468 None:intergenic 35.0%
AGAGAAGGAAGAGTAGTATA+AGG - chr5_2:25078518-25078537 None:intergenic 35.0%
! TTTAGAGTACTTGTAGTGGT+GGG + chr5_2:25078754-25078773 Msa0764700:CDS 35.0%
TTTAGTAGCTTAGTTAGTGG+TGG + chr5_2:25078796-25078815 Msa0764700:CDS 35.0%
GACAAACAACTACAAGACAT+TGG + chr5_2:25078827-25078846 Msa0764700:CDS 35.0%
CATGATGAACAAGTTGTTGT+TGG + chr5_2:25078865-25078884 Msa0764700:CDS 35.0%
! AGTTGTTGTTGGTAAAGACA+AGG + chr5_2:25078876-25078895 Msa0764700:CDS 35.0%
! GTTGTTGTTGGTAAAGACAA+GGG + chr5_2:25078877-25078896 Msa0764700:CDS 35.0%
AGTAAAAGAAGAGTTCCCAA+TGG + chr5_2:25078943-25078962 Msa0764700:CDS 35.0%
CCAATTGAACCATAACTCAA+AGG - chr5_2:25079286-25079305 None:intergenic 35.0%
! CCTTTGAGTTATGGTTCAAT+TGG + chr5_2:25079283-25079302 Msa0764700:intron 35.0%
! ATGGTTCAATTGGTTTATCG+TGG + chr5_2:25079293-25079312 Msa0764700:intron 35.0%
AAAACCGATTGAACCAATTG+TGG + chr5_2:25079404-25079423 Msa0764700:intron 35.0%
! TGAACCACAATTGGTTCAAT+CGG - chr5_2:25079411-25079430 None:intergenic 35.0%
GATTAGATTAGGGTTCAAAC+AGG + chr5_2:25079471-25079490 Msa0764700:intron 35.0%
TAATTGACCAGTTGTTTGGT+TGG - chr5_2:25079503-25079522 None:intergenic 35.0%
CCAATAATTGACCAGTTGTT+TGG - chr5_2:25079507-25079526 None:intergenic 35.0%
CCAAACAACTGGTCAATTAT+TGG + chr5_2:25079504-25079523 Msa0764700:intron 35.0%
!! ACTAGTTGATTTGACCTCTT+CGG + chr5_2:25079531-25079550 Msa0764700:intron 35.0%
CATTGCAATAATATATGCGG+GGG - chr5_2:25080561-25080580 None:intergenic 35.0%
!!! TAACATTTTTTCAGGAGAGC+TGG + chr5_2:25080691-25080710 Msa0764700:intron 35.0%
! CCAGAAATTCTAAGAACCTT+AGG - chr5_2:25080829-25080848 None:intergenic 35.0%
CCTAAGGTTCTTAGAATTTC+TGG + chr5_2:25080826-25080845 Msa0764700:three_prime_UTR 35.0%
!! AATTTCTGGCTTGTATGCAA+AGG + chr5_2:25080840-25080859 Msa0764700:three_prime_UTR 35.0%
!! ATTTCTGGCTTGTATGCAAA+GGG + chr5_2:25080841-25080860 Msa0764700:three_prime_UTR 35.0%
! TTGTTAAGGGAAGAGTTTTG+TGG + chr5_2:25080888-25080907 Msa0764700:three_prime_UTR 35.0%
!! AAGAGTTTTGTGGCCTTAAA+AGG + chr5_2:25080898-25080917 Msa0764700:three_prime_UTR 35.0%
!! AGAGTTTTGTGGCCTTAAAA+GGG + chr5_2:25080899-25080918 Msa0764700:three_prime_UTR 35.0%
! CTTTCCTCTTTTCCCTTTTA+AGG - chr5_2:25080914-25080933 None:intergenic 35.0%
TGGAGAGTCACAAATTTCAA+TGG + chr5_2:25081057-25081076 Msa0764700:three_prime_UTR 35.0%
TTAGGACTATATGTTTGAGG+GGG + chr5_2:25081193-25081212 Msa0764700:three_prime_UTR 35.0%
AGGGGGGAAAAAATATATCT+TGG + chr5_2:25081210-25081229 Msa0764700:three_prime_UTR 35.0%
!! GATTTTTAAGCGTGACATGG+AGG - chr5_2:25078381-25078400 None:intergenic 40.0%
GAAGGAAGAGTAGTATAAGG+AGG - chr5_2:25078515-25078534 None:intergenic 40.0%
AGAGAAAATAGAGGGAGAGA+AGG - chr5_2:25078533-25078552 None:intergenic 40.0%
!! CTTTAGAGTACTTGTAGTGG+TGG + chr5_2:25078753-25078772 Msa0764700:CDS 40.0%
! TTAGAGTACTTGTAGTGGTG+GGG + chr5_2:25078755-25078774 Msa0764700:CDS 40.0%
!!! GGTGGGGTTTGTTTGTTTTA+TGG + chr5_2:25078771-25078790 Msa0764700:CDS 40.0%
TGTCAACCATGATGCACAAT+TGG + chr5_2:25078906-25078925 Msa0764700:CDS 40.0%
TGAAGTCCAATTGTGCATCA+TGG - chr5_2:25078915-25078934 None:intergenic 40.0%
TGAATAGGATCTGGTCCATT+GGG - chr5_2:25078961-25078980 None:intergenic 40.0%
AACCTGTTGTGAATAGGATC+TGG - chr5_2:25078970-25078989 None:intergenic 40.0%
! CTCATCTGACCTTTGAGTTA+TGG + chr5_2:25079274-25079293 Msa0764700:intron 40.0%
GATCAAACCAACCAAACAAC+TGG + chr5_2:25079493-25079512 Msa0764700:intron 40.0%
GGTCAAGTTTCCAATATAGC+AGG - chr5_2:25079881-25079900 None:intergenic 40.0%
TCAGTTGACTAAGTTTGACC+AGG - chr5_2:25080733-25080752 None:intergenic 40.0%
CAAAGGGCTAATCACACTTA+AGG + chr5_2:25080857-25080876 Msa0764700:three_prime_UTR 40.0%
AAGATGGGGTTAGACAGAAA+TGG + chr5_2:25081037-25081056 Msa0764700:three_prime_UTR 40.0%
TAGGACTATATGTTTGAGGG+GGG + chr5_2:25081194-25081213 Msa0764700:three_prime_UTR 40.0%
AGTAGCTTAGTTAGTGGTGG+AGG + chr5_2:25078799-25078818 Msa0764700:CDS 45.0%
! TTAGTTAGTGGTGGAGGAAC+AGG + chr5_2:25078805-25078824 Msa0764700:CDS 45.0%
GTGAATAGGATCTGGTCCAT+TGG - chr5_2:25078962-25078981 None:intergenic 45.0%
GACCAGATCCTATTCACAAC+AGG + chr5_2:25078965-25078984 Msa0764700:CDS 45.0%
TTGTCAAGTACCCTGGTAGT+TGG + chr5_2:25080481-25080500 Msa0764700:intron 45.0%
TTTGTGAGCTCCAACTACCA+GGG - chr5_2:25080494-25080513 None:intergenic 45.0%
! TTTTGTGAGCTCCAACTACC+AGG - chr5_2:25080495-25080514 None:intergenic 45.0%
TTCAGGAGAGCTGGAAATTC+TGG + chr5_2:25080700-25080719 Msa0764700:intron 45.0%
GTTGACTAAGTTTGACCAGG+AGG - chr5_2:25080730-25080749 None:intergenic 45.0%
! GTGGCCTTAAAAGGGAAAAG+AGG + chr5_2:25080907-25080926 Msa0764700:three_prime_UTR 45.0%
! GAAGGATTAAGGGGTGTGGT+TGG - chr5_2:25078462-25078481 None:intergenic 50.0%
CTACGAAGGATTAAGGGGTG+TGG - chr5_2:25078466-25078485 None:intergenic 50.0%
AATAATATATGCGGGGGCCG+GGG - chr5_2:25080555-25080574 None:intergenic 50.0%
CAATAATATATGCGGGGGCC+GGG - chr5_2:25080556-25080575 None:intergenic 50.0%
GCAATAATATATGCGGGGGC+CGG - chr5_2:25080557-25080576 None:intergenic 50.0%
! TGAGGCTGGCTCAATTGGTA+GGG - chr5_2:25080585-25080604 None:intergenic 50.0%
TACCAATTGAGCCAGCCTCA+CGG + chr5_2:25080585-25080604 Msa0764700:intron 50.0%
GGAAATTCTGGTAGACCTCC+TGG + chr5_2:25080712-25080731 Msa0764700:CDS 50.0%
! ATGTGGAGAAGTGGGGTGTC+CGG + chr5_2:25080521-25080540 Msa0764700:intron 55.0%
! GTGAGGCTGGCTCAATTGGT+AGG - chr5_2:25080586-25080605 None:intergenic 55.0%
ACCAATTGAGCCAGCCTCAC+GGG + chr5_2:25080586-25080605 Msa0764700:intron 55.0%
! TGTGGAGAAGTGGGGTGTCC+GGG + chr5_2:25080522-25080541 Msa0764700:intron 60.0%
CCAATTGAGCCAGCCTCACG+GGG + chr5_2:25080587-25080606 Msa0764700:intron 60.0%
! GTGGAGAAGTGGGGTGTCCG+GGG + chr5_2:25080523-25080542 Msa0764700:intron 65.0%
! CCCCGTGAGGCTGGCTCAAT+TGG - chr5_2:25080590-25080609 None:intergenic 65.0%
ATTGGTCCCTGGTCCCCGTG+AGG - chr5_2:25080603-25080622 None:intergenic 65.0%
GGTGTCCGGGGTTCGAACCC+CGG + chr5_2:25080535-25080554 Msa0764700:intron 70.0%
AGCCAGCCTCACGGGGACCA+GGG + chr5_2:25080594-25080613 Msa0764700:intron 70.0%
GAGCCAGCCTCACGGGGACC+AGG + chr5_2:25080593-25080612 Msa0764700:intron 75.0%
GTCCCTGGTCCCCGTGAGGC+TGG - chr5_2:25080599-25080618 None:intergenic 75.0%
! GGGGGCCGGGGTTCGAACCC+CGG - chr5_2:25080543-25080562 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 25078278 25081279 25078278 ID=Msa0764700;Name=Msa0764700
chr5_2 mRNA 25078278 25081279 25078278 ID=Msa0764700-mRNA-1;Parent=Msa0764700;Name=Msa0764700-mRNA-1;_AED=0.00;_eAED=0.00;_QI=425|1|1|1|1|1|2|535|107
chr5_2 exon 25078278 25078986 25078278 ID=Msa0764700-mRNA-1:exon:10091;Parent=Msa0764700-mRNA-1
chr5_2 exon 25080705 25081279 25080705 ID=Msa0764700-mRNA-1:exon:10092;Parent=Msa0764700-mRNA-1
chr5_2 five_prime_UTR 25078278 25078702 25078278 ID=Msa0764700-mRNA-1:five_prime_utr;Parent=Msa0764700-mRNA-1
chr5_2 CDS 25078703 25078986 25078703 ID=Msa0764700-mRNA-1:cds;Parent=Msa0764700-mRNA-1
chr5_2 CDS 25080705 25080744 25080705 ID=Msa0764700-mRNA-1:cds;Parent=Msa0764700-mRNA-1
chr5_2 three_prime_UTR 25080745 25081279 25080745 ID=Msa0764700-mRNA-1:three_prime_utr;Parent=Msa0764700-mRNA-1
Gene Sequence

>Msa0764700

ATGGATACTAATACTAGTAGTAGTAGTAGTTATTTCTTTTCAAGGTTTTTCTTTAGAGTACTTGTAGTGGTGGGGTTTGTTTGTTTTATGGTTTTTAGTAGCTTAGTTAGTGGTGGAGGAACAGGACAAACAACTACAAGACATTGGTCAGAAACACTCAAACATGATGAACAAGTTGTTGTTGGTAAAGACAAGGGTGTTGTTGTCAACCATGATGCACAATTGGACTTCAACTATATGAGTAAAAGAAGAGTTCCCAATGGACCAGATCCTATTCACAACAGGAGAGCTGGAAATTCTGGTAGACCTCCTGGTCAAACTTAG
Protein sequence

>Msa0764700

MDTNTSSSSSYFFSRFFFRVLVVVGFVCFMVFSSLVSGGGTGQTTTRHWSETLKHDEQVVVGKDKGVVVNHDAQLDFNYMSKRRVPNGPDPIHNRRAGNSGRPPGQT*