AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705180 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0705180 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0705180 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0705180 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0705180 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0705180 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0705180 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0705180 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0705180 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0705180 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0705180 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0705180 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0705180 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0705180 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0705180 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0705180 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705180 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0705180 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0705180 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0705180 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0705180 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0705180 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0705180 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0705180 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0705180 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0705180 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0705180 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0705180 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0705180 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0705180 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0705180 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0705180 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTGGCGAGCTGCTTC+CGG 0.229871 4_4:+77724825 None:intergenic
CTTTGTGGCGAGCTGCTTCC+GGG 0.231058 4_4:+77724826 None:intergenic
CAGTGCTGTTTCTGCTCTTC+AGG 0.258870 4_4:-77724615 Msa0705180:CDS
TCTTGCAAGTTGAATGTCCT+TGG 0.320782 4_4:+77724511 None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG 0.333816 4_4:+77724746 None:intergenic
CTTGCAAGTTGAATGTCCTT+GGG 0.355623 4_4:+77724512 None:intergenic
GGAAGCAGCTGAAGCTTACT+TGG 0.369838 4_4:-77724594 Msa0705180:CDS
GAGCAGAAACAGCACTGCTC+TGG 0.422418 4_4:+77724621 None:intergenic
TGATTTCCGAGCGGCCTTTG+TGG 0.424857 4_4:+77724811 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 4_4:+77724785 None:intergenic
GACGTTGGAATGGAAGTTTA+CGG 0.458530 4_4:+77724687 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.461897 4_4:-77724797 Msa0705180:CDS
GCAGCTGAAGCTTACTTGGT+TGG 0.472209 4_4:-77724590 Msa0705180:CDS
CCACCGGCGGCAAGGCTCCC+CGG 0.480154 4_4:-77724844 Msa0705180:CDS
AGCTCGCCACAAAGGCCGCT+CGG 0.491073 4_4:-77724817 Msa0705180:CDS
GGCCGCTCGGAAATCAGCTC+CGG 0.498921 4_4:-77724804 Msa0705180:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.499925 4_4:+77724778 None:intergenic
CTTCCGGGGAGCCTTGCCGC+CGG 0.507682 4_4:+77724841 None:intergenic
GGACTTGCGAGCTGTTTGCT+TGG 0.512299 4_4:+77724865 None:intergenic
TCGCAAGTCCACCGGCGGCA+AGG 0.530180 4_4:-77724852 Msa0705180:CDS
CCGGGGAGCCTTGCCGCCGG+TGG 0.548377 4_4:+77724844 None:intergenic
CAAACAGCTCGCAAGTCCAC+CGG 0.573307 4_4:-77724860 Msa0705180:CDS
TTTGTGGCGAGCTGCTTCCG+GGG 0.576886 4_4:+77724827 None:intergenic
AAGAAGAATCAGAGGCGAGA+GGG 0.597241 4_4:-77724492 Msa0705180:CDS
TCACGAACAAGACGTTGGAA+TGG 0.598115 4_4:+77724677 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.599225 4_4:-77724794 Msa0705180:CDS
CCGGAAGCAGCTCGCCACAA+AGG 0.611340 4_4:-77724825 Msa0705180:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 4_4:+77724672 None:intergenic
GAGAGTTACAATCATGCCCA+AGG 0.637894 4_4:-77724528 Msa0705180:CDS
TCTTAGCATGAATTGCACAA+AGG 0.643725 4_4:+77724549 None:intergenic
CAAGAAGAATCAGAGGCGAG+AGG 0.648850 4_4:-77724493 Msa0705180:CDS
ACAGCTCGCAAGTCCACCGG+CGG 0.655092 4_4:-77724857 Msa0705180:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.655500 4_4:-77724500 Msa0705180:CDS
AAGCCACACAGATTCAGACC+TGG 0.672238 4_4:-77724764 Msa0705180:CDS
GTTCCAGGTCTGAATCTGTG+TGG 0.688220 4_4:+77724761 None:intergenic
CGCCGGAGCTGATTTCCGAG+CGG 0.728257 4_4:+77724802 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCTTAGCATGAATTGCACAA+AGG + chr4_4:77724816-77724835 None:intergenic 35.0%
GACGTTGGAATGGAAGTTTA+CGG + chr4_4:77724678-77724697 None:intergenic 40.0%
! CTTGCAAGTTGAATGTCCTT+GGG + chr4_4:77724853-77724872 None:intergenic 40.0%
! TCTTGCAAGTTGAATGTCCT+TGG + chr4_4:77724854-77724873 None:intergenic 40.0%
CAACTTGCAAGAAGAATCAG+AGG - chr4_4:77724862-77724881 Msa0705180:CDS 40.0%
TCTCTCAAAGCAACTGTTCC+AGG + chr4_4:77724619-77724638 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr4_4:77724688-77724707 None:intergenic 45.0%
CGATTTCACGAACAAGACGT+TGG + chr4_4:77724693-77724712 None:intergenic 45.0%
GAGAGTTACAATCATGCCCA+AGG - chr4_4:77724834-77724853 Msa0705180:CDS 45.0%
AAGAAGAATCAGAGGCGAGA+GGG - chr4_4:77724870-77724889 Msa0705180:CDS 45.0%
AAGCCACACAGATTCAGACC+TGG - chr4_4:77724598-77724617 Msa0705180:CDS 50.0%
GTTCCAGGTCTGAATCTGTG+TGG + chr4_4:77724604-77724623 None:intergenic 50.0%
!! CAGTGCTGTTTCTGCTCTTC+AGG - chr4_4:77724747-77724766 Msa0705180:CDS 50.0%
GGAAGCAGCTGAAGCTTACT+TGG - chr4_4:77724768-77724787 Msa0705180:CDS 50.0%
GCAGCTGAAGCTTACTTGGT+TGG - chr4_4:77724772-77724791 Msa0705180:CDS 50.0%
CAAGAAGAATCAGAGGCGAG+AGG - chr4_4:77724869-77724888 Msa0705180:CDS 50.0%
GGACTTGCGAGCTGTTTGCT+TGG + chr4_4:77724500-77724519 None:intergenic 55.0%
CAAACAGCTCGCAAGTCCAC+CGG - chr4_4:77724502-77724521 Msa0705180:CDS 55.0%
TGATTTCCGAGCGGCCTTTG+TGG + chr4_4:77724554-77724573 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr4_4:77724587-77724606 None:intergenic 55.0%
GAGCAGAAACAGCACTGCTC+TGG + chr4_4:77724744-77724763 None:intergenic 55.0%
TTTGTGGCGAGCTGCTTCCG+GGG + chr4_4:77724538-77724557 None:intergenic 60.0%
CTTTGTGGCGAGCTGCTTCC+GGG + chr4_4:77724539-77724558 None:intergenic 60.0%
CCTTTGTGGCGAGCTGCTTC+CGG + chr4_4:77724540-77724559 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + chr4_4:77724580-77724599 None:intergenic 60.0%
ACAGCTCGCAAGTCCACCGG+CGG - chr4_4:77724505-77724524 Msa0705180:CDS 65.0%
CCGGAAGCAGCTCGCCACAA+AGG - chr4_4:77724537-77724556 Msa0705180:CDS 65.0%
AGCTCGCCACAAAGGCCGCT+CGG - chr4_4:77724545-77724564 Msa0705180:CDS 65.0%
GGCCGCTCGGAAATCAGCTC+CGG - chr4_4:77724558-77724577 Msa0705180:CDS 65.0%
! CGCCGGAGCTGATTTCCGAG+CGG + chr4_4:77724563-77724582 None:intergenic 65.0%
CGGAAATCAGCTCCGGCGAC+CGG - chr4_4:77724565-77724584 Msa0705180:CDS 65.0%
AAATCAGCTCCGGCGACCGG+AGG - chr4_4:77724568-77724587 Msa0705180:CDS 65.0%
TCGCAAGTCCACCGGCGGCA+AGG - chr4_4:77724510-77724529 Msa0705180:CDS 70.0%
CTTCCGGGGAGCCTTGCCGC+CGG + chr4_4:77724524-77724543 None:intergenic 75.0%
! CCACCGGCGGCAAGGCTCCC+CGG - chr4_4:77724518-77724537 Msa0705180:CDS 80.0%
! CCGGGGAGCCTTGCCGCCGG+TGG + chr4_4:77724521-77724540 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 77724487 77724897 77724487 ID=Msa0705180;Name=Msa0705180
chr4_4 mRNA 77724487 77724897 77724487 ID=Msa0705180-mRNA-1;Parent=Msa0705180;Name=Msa0705180-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|136
chr4_4 exon 77724487 77724897 77724487 ID=Msa0705180-mRNA-1:exon:24751;Parent=Msa0705180-mRNA-1
chr4_4 CDS 77724487 77724897 77724487 ID=Msa0705180-mRNA-1:cds;Parent=Msa0705180-mRNA-1
Gene Sequence

>Msa0705180

ATGGCACGTACCAAGCAAACAGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAGCTCGCCACAAAGGCCGCTCGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAGGAAGCAGCTGAAGCTTACTTGGTTGGACTCTTTGAAGACACAAACCTTTGTGCAATTCATGCTAAGAGAGTTACAATCATGCCCAAGGACATTCAACTTGCAAGAAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>Msa0705180

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*