AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0659200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659200 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659200 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0659200 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0659200 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0659200 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0659200 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0659200 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0659200 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0659200 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0659200 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0659200 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0659200 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0659200 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0659200 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0659200 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0659200 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659200 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659200 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659200 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659200 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659200 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659200 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659200 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0659200 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0659200 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0659200 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0659200 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0659200 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0659200 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0659200 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0659200 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0659200 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTGGCGAGCTGCTTC+CGG 0.229871 4_3:+69496919 None:intergenic
CTTTGTGGCGAGCTGCTTCC+GGG 0.231058 4_3:+69496920 None:intergenic
CAGTGCTGTTTCTGCTCTTC+AGG 0.258870 4_3:-69496709 Msa0659200:CDS
TCTTGCAAGTTGAATGTCCT+TGG 0.320782 4_3:+69496605 None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG 0.333816 4_3:+69496840 None:intergenic
CTTGCAAGTTGAATGTCCTT+GGG 0.355623 4_3:+69496606 None:intergenic
GGAAGCAGCTGAAGCTTACT+TGG 0.369838 4_3:-69496688 Msa0659200:CDS
GAGCAGAAACAGCACTGCTC+TGG 0.422418 4_3:+69496715 None:intergenic
TGATTTCCGAGCGGCCTTTG+TGG 0.424857 4_3:+69496905 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 4_3:+69496879 None:intergenic
GACGTTGGAATGGAAGTTTA+CGG 0.458530 4_3:+69496781 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.461897 4_3:-69496891 Msa0659200:CDS
GCAGCTGAAGCTTACTTGGT+TGG 0.472209 4_3:-69496684 Msa0659200:CDS
CCACCGGCGGCAAGGCTCCC+CGG 0.480154 4_3:-69496938 Msa0659200:CDS
AGCTCGCCACAAAGGCCGCT+CGG 0.491073 4_3:-69496911 Msa0659200:CDS
GGCCGCTCGGAAATCAGCTC+CGG 0.498921 4_3:-69496898 Msa0659200:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.499925 4_3:+69496872 None:intergenic
CTTCCGGGGAGCCTTGCCGC+CGG 0.507682 4_3:+69496935 None:intergenic
GGACTTGCGAGCTGTTTGCT+TGG 0.512299 4_3:+69496959 None:intergenic
TCGCAAGTCCACCGGCGGCA+AGG 0.530180 4_3:-69496946 Msa0659200:CDS
CCGGGGAGCCTTGCCGCCGG+TGG 0.548377 4_3:+69496938 None:intergenic
CAAACAGCTCGCAAGTCCAC+CGG 0.573307 4_3:-69496954 Msa0659200:CDS
TTTGTGGCGAGCTGCTTCCG+GGG 0.576886 4_3:+69496921 None:intergenic
AAGAAGAATCAGAGGCGAGA+GGG 0.597241 4_3:-69496586 Msa0659200:CDS
TCACGAACAAGACGTTGGAA+TGG 0.598115 4_3:+69496771 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.599225 4_3:-69496888 Msa0659200:CDS
CCGGAAGCAGCTCGCCACAA+AGG 0.611340 4_3:-69496919 Msa0659200:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 4_3:+69496766 None:intergenic
GAGAGTTACAATCATGCCCA+AGG 0.637894 4_3:-69496622 Msa0659200:CDS
TCTTAGCATGAATTGCACAA+AGG 0.643725 4_3:+69496643 None:intergenic
CAAGAAGAATCAGAGGCGAG+AGG 0.648850 4_3:-69496587 Msa0659200:CDS
ACAGCTCGCAAGTCCACCGG+CGG 0.655092 4_3:-69496951 Msa0659200:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.655500 4_3:-69496594 Msa0659200:CDS
AAGCCACACAGATTCAGACC+TGG 0.672238 4_3:-69496858 Msa0659200:CDS
GTTCCAGGTCTGAATCTGTG+TGG 0.688220 4_3:+69496855 None:intergenic
CGCCGGAGCTGATTTCCGAG+CGG 0.728257 4_3:+69496896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCTTAGCATGAATTGCACAA+AGG + chr4_3:69496910-69496929 None:intergenic 35.0%
GACGTTGGAATGGAAGTTTA+CGG + chr4_3:69496772-69496791 None:intergenic 40.0%
! CTTGCAAGTTGAATGTCCTT+GGG + chr4_3:69496947-69496966 None:intergenic 40.0%
! TCTTGCAAGTTGAATGTCCT+TGG + chr4_3:69496948-69496967 None:intergenic 40.0%
CAACTTGCAAGAAGAATCAG+AGG - chr4_3:69496956-69496975 Msa0659200:CDS 40.0%
TCTCTCAAAGCAACTGTTCC+AGG + chr4_3:69496713-69496732 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr4_3:69496782-69496801 None:intergenic 45.0%
CGATTTCACGAACAAGACGT+TGG + chr4_3:69496787-69496806 None:intergenic 45.0%
GAGAGTTACAATCATGCCCA+AGG - chr4_3:69496928-69496947 Msa0659200:CDS 45.0%
AAGAAGAATCAGAGGCGAGA+GGG - chr4_3:69496964-69496983 Msa0659200:CDS 45.0%
AAGCCACACAGATTCAGACC+TGG - chr4_3:69496692-69496711 Msa0659200:CDS 50.0%
GTTCCAGGTCTGAATCTGTG+TGG + chr4_3:69496698-69496717 None:intergenic 50.0%
!! CAGTGCTGTTTCTGCTCTTC+AGG - chr4_3:69496841-69496860 Msa0659200:CDS 50.0%
GGAAGCAGCTGAAGCTTACT+TGG - chr4_3:69496862-69496881 Msa0659200:CDS 50.0%
GCAGCTGAAGCTTACTTGGT+TGG - chr4_3:69496866-69496885 Msa0659200:CDS 50.0%
CAAGAAGAATCAGAGGCGAG+AGG - chr4_3:69496963-69496982 Msa0659200:CDS 50.0%
GGACTTGCGAGCTGTTTGCT+TGG + chr4_3:69496594-69496613 None:intergenic 55.0%
CAAACAGCTCGCAAGTCCAC+CGG - chr4_3:69496596-69496615 Msa0659200:CDS 55.0%
TGATTTCCGAGCGGCCTTTG+TGG + chr4_3:69496648-69496667 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr4_3:69496681-69496700 None:intergenic 55.0%
GAGCAGAAACAGCACTGCTC+TGG + chr4_3:69496838-69496857 None:intergenic 55.0%
TTTGTGGCGAGCTGCTTCCG+GGG + chr4_3:69496632-69496651 None:intergenic 60.0%
CTTTGTGGCGAGCTGCTTCC+GGG + chr4_3:69496633-69496652 None:intergenic 60.0%
CCTTTGTGGCGAGCTGCTTC+CGG + chr4_3:69496634-69496653 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + chr4_3:69496674-69496693 None:intergenic 60.0%
ACAGCTCGCAAGTCCACCGG+CGG - chr4_3:69496599-69496618 Msa0659200:CDS 65.0%
CCGGAAGCAGCTCGCCACAA+AGG - chr4_3:69496631-69496650 Msa0659200:CDS 65.0%
AGCTCGCCACAAAGGCCGCT+CGG - chr4_3:69496639-69496658 Msa0659200:CDS 65.0%
GGCCGCTCGGAAATCAGCTC+CGG - chr4_3:69496652-69496671 Msa0659200:CDS 65.0%
! CGCCGGAGCTGATTTCCGAG+CGG + chr4_3:69496657-69496676 None:intergenic 65.0%
CGGAAATCAGCTCCGGCGAC+CGG - chr4_3:69496659-69496678 Msa0659200:CDS 65.0%
AAATCAGCTCCGGCGACCGG+AGG - chr4_3:69496662-69496681 Msa0659200:CDS 65.0%
TCGCAAGTCCACCGGCGGCA+AGG - chr4_3:69496604-69496623 Msa0659200:CDS 70.0%
CTTCCGGGGAGCCTTGCCGC+CGG + chr4_3:69496618-69496637 None:intergenic 75.0%
! CCACCGGCGGCAAGGCTCCC+CGG - chr4_3:69496612-69496631 Msa0659200:CDS 80.0%
! CCGGGGAGCCTTGCCGCCGG+TGG + chr4_3:69496615-69496634 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69496581 69496991 69496581 ID=Msa0659200;Name=Msa0659200
chr4_3 mRNA 69496581 69496991 69496581 ID=Msa0659200-mRNA-1;Parent=Msa0659200;Name=Msa0659200-mRNA-1;_AED=0.41;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|136
chr4_3 exon 69496581 69496991 69496581 ID=Msa0659200-mRNA-1:exon:20420;Parent=Msa0659200-mRNA-1
chr4_3 CDS 69496581 69496991 69496581 ID=Msa0659200-mRNA-1:cds;Parent=Msa0659200-mRNA-1
Gene Sequence

>Msa0659200

ATGGCACGTACCAAGCAAACAGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAGCTCGCCACAAAGGCCGCTCGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAGGAAGCAGCTGAAGCTTACTTGGTTGGACTCTTTGAAGACACAAACCTTTGTGCAATTCATGCTAAGAGAGTTACAATCATGCCCAAGGACATTCAACTTGCAAGAAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>Msa0659200

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*