AlfalfaGEDB | Msa1034300

M. sativa cultivar zhongmu-4 / Msa1034300

KEGG: K10728
GO: GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034300	RHN47818.1	77.143	70	13	1	2	71	4	70	3.11e-28	111

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034300	A0A396H4T6	77.143	70	13	1	2	71	4	70	1.48e-28	111

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006070	Msa1034300	0.814283	1.667435e-51	-8.615850e-47
Msa0011170	Msa1034300	0.817765	2.783304e-52	-8.615850e-47
Msa0011960	Msa1034300	0.800199	1.612192e-48	-8.615850e-47
Msa0014710	Msa1034300	0.837710	4.426674e-57	-8.615850e-47
Msa0037880	Msa1034300	0.807460	5.001428e-50	-8.615850e-47
Msa0078900	Msa1034300	0.808712	2.707178e-50	-8.615850e-47
Msa0114060	Msa1034300	0.806543	7.816551e-50	-8.615850e-47
Msa0116770	Msa1034300	0.803224	3.861352e-49	-8.615850e-47
Msa0136070	Msa1034300	0.806156	9.430309e-50	-8.615850e-47
Msa0140390	Msa1034300	0.815810	7.638770e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034300	MtrunA17_Chr7g0257121	77.143	70	13	1	2	71	4	70	2.86e-32	111
Msa1034300	MtrunA17_Chr1g0159641	53.571	56	26	0	3	58	5	60	9.02e-16	69.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1034300	AT1G77320.1	43.396	53	30	0	2	54	4	56	3.86e-11	57.4
Msa1034300	AT1G77320.3	43.396	53	30	0	2	54	4	56	4.14e-11	57.4

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Find 135 sgRNAs with CRISPR-Local

Find 348 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTGAGAACCACTTCA+AGG	0.518932	7_1:+69744420	Msa1034300:three_prime_UTR
AAAGAAGAATCTAGGAATGA+AGG	0.461492	7_1:+69744018	Msa1034300:three_prime_UTR
AAAGAGACGACAAAAGGAGA+TGG	0.591489	7_1:+69744141	Msa1034300:three_prime_UTR
AAAGCCGTTGAAGAGAATGG+CGG	0.740750	7_1:+69738938	Msa1034300:CDS
AACAAGTCCATGTTCTACAA+CGG	0.651160	7_1:+69743619	Msa1034300:three_prime_UTR
AACATTGAGAACCACTTCAA+GGG	0.524205	7_1:+69744421	Msa1034300:three_prime_UTR
AACCAATAGTTACTTATGAA+TGG	0.439724	7_1:+69741070	Msa1034300:three_prime_UTR
AACCATTCATAAGTAACTAT+TGG	0.328810	7_1:-69741072	None:intergenic
AACTAGTATATTTGAATCTG+CGG	0.510360	7_1:-69742952	None:intergenic
AAGAAAGCAATGGAGGCATT+TGG	0.384504	7_1:+69744331	Msa1034300:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATGTCGAAGTTATTTGA+AGG	+	chr7_1:69738857-69738876	Msa1034300:exon	30.0%
AGATGTCGAAGTTATTTGAA+GGG	+	chr7_1:69738858-69738877	Msa1034300:exon	30.0%
TCAAAACGTACGTAATTTCT+TGG	+	chr7_1:69739027-69739046	Msa1034300:intron	30.0%
GAATTAATGATTGAAATGGG+GGG	+	chr7_1:69740031-69740050	Msa1034300:three_prime_UTR	30.0%
TTGTCATCGTGAAAAATGTT+TGG	+	chr7_1:69740089-69740108	Msa1034300:three_prime_UTR	30.0%
TGTCATCGTGAAAAATGTTT+GGG	+	chr7_1:69740090-69740109	Msa1034300:three_prime_UTR	30.0%
GTATGAATAAAACAGAAGGA+AGG	-	chr7_1:69740227-69740246	None:intergenic	30.0%
TGTAAAAATCATGTAGTCCA+AGG	+	chr7_1:69740346-69740365	Msa1034300:intron	30.0%
GTAAAAATCATGTAGTCCAA+GGG	+	chr7_1:69740347-69740366	Msa1034300:intron	30.0%
TAAGTAATCATCTAGATGCA+GGG	-	chr7_1:69740873-69740892	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	69738824	69744749	69738824	ID=Msa1034300;Name=Msa1034300
chr7_1	mRNA	69738824	69744749	69738824	ID=Msa1034300-mRNA-1;Parent=Msa1034300;Name=Msa1034300-mRNA-1;_AED=0.32;_eAED=0.32;_QI=36\|0.87\|1\|1\|0.62\|0.77\|9\|1888\|81
chr7_1	exon	69738824	69739033	69738824	ID=Msa1034300-mRNA-1:exon:16487;Parent=Msa1034300-mRNA-1
chr7_1	exon	69739532	69739653	69739532	ID=Msa1034300-mRNA-1:exon:16488;Parent=Msa1034300-mRNA-1
chr7_1	exon	69740019	69740126	69740019	ID=Msa1034300-mRNA-1:exon:16489;Parent=Msa1034300-mRNA-1
chr7_1	exon	69741048	69741192	69741048	ID=Msa1034300-mRNA-1:exon:16490;Parent=Msa1034300-mRNA-1
chr7_1	exon	69741271	69741362	69741271	ID=Msa1034300-mRNA-1:exon:16491;Parent=Msa1034300-mRNA-1
chr7_1	exon	69742527	69742620	69742527	ID=Msa1034300-mRNA-1:exon:16492;Parent=Msa1034300-mRNA-1
chr7_1	exon	69742820	69743306	69742820	ID=Msa1034300-mRNA-1:exon:16493;Parent=Msa1034300-mRNA-1
chr7_1	exon	69743530	69743672	69743530	ID=Msa1034300-mRNA-1:exon:16494;Parent=Msa1034300-mRNA-1
chr7_1	exon	69743981	69744749	69743981	ID=Msa1034300-mRNA-1:exon:16495;Parent=Msa1034300-mRNA-1
chr7_1	five_prime_UTR	69738824	69738859	69738824	ID=Msa1034300-mRNA-1:five_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	CDS	69738860	69739033	69738860	ID=Msa1034300-mRNA-1:cds;Parent=Msa1034300-mRNA-1
chr7_1	CDS	69739532	69739603	69739532	ID=Msa1034300-mRNA-1:cds;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69739604	69739653	69739604	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69740019	69740126	69740019	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69741048	69741192	69741048	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69741271	69741362	69741271	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69742527	69742620	69742527	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69742820	69743306	69742820	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69743530	69743672	69743530	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1
chr7_1	three_prime_UTR	69743981	69744749	69743981	ID=Msa1034300-mRNA-1:three_prime_utr;Parent=Msa1034300-mRNA-1

Gene Sequence	>Msa1034300 ATGTCGAAGTTATTTGAAGGGTTTAACGTGTTCCTATCGCGACACCTATTTCCGCCTGACAAATTTGATGCGGTTCTCAAAGCCGTTGAAGAGAATGGCGGTCAAGTTCACATCGGTTTTAATCTTTCCCGCAATGGCGAAAACGATTATCATATCATTTCTTGCCGTCAAAACGTTATAGATGTGTTCTTCAATGTGCTAAAGAAGGAAGGCCTCTGCCCAAACAAGCCTTCACTTGCTGCTTAA
Protein sequence	>Msa1034300 MSKLFEGFNVFLSRHLFPPDKFDAVLKAVEENGGQVHIGFNLSRNGENDYHIISCRQNVIDVFFNVLKKEGLCPNKPSLAA*