AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0927280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACCAGTTTCATTGATTT+TGG 0.172670 6_2:+71543701 Msa0927280:intron
TGTTATATTGATTGGGAATA+TGG 0.211216 6_2:+71544026 Msa0927280:exon
AGTTTGGTGAAACCCGTATT+TGG 0.217150 6_2:+71543581 Msa0927280:five_prime_UTR
TTCTTGCAGGGGAGCGGCTG+TGG 0.263658 6_2:+71543971 Msa0927280:intron
ATTATCTTTGTTATATTGAT+TGG 0.269667 6_2:+71544018 Msa0927280:five_prime_UTR
GAAAGTGATTATGGAGGATT+AGG 0.272658 6_2:+71543482 Msa0927280:five_prime_UTR
AGGGAAACCCACCTTAAAAT+TGG 0.272876 6_2:-71543053 None:intergenic
CCAAGAGGAAGAAGGAAAAT+TGG 0.288925 6_2:+71542945 Msa0927280:five_prime_UTR
CTTAAGTTCAAGTTCATTGC+AGG 0.318119 6_2:+71544288 Msa0927280:three_prime_UTR
ATCAAAACAACTTATATTCA+TGG 0.333826 6_2:-71543786 None:intergenic
AAGAAGGGAGGTCGAGGGAG+AGG 0.341404 6_2:+71542901 Msa0927280:five_prime_UTR
GAGAGGAGAAGAAGAGGAAA+CGG 0.348550 6_2:+71542918 Msa0927280:five_prime_UTR
AAGCTGACAGAAAGTGATTA+TGG 0.355367 6_2:+71543473 Msa0927280:five_prime_UTR
GAAGTGGATAAATTGTAATT+AGG 0.373522 6_2:+71543514 Msa0927280:five_prime_UTR
AAGGAAAAGAAGGGAGGTCG+AGG 0.373699 6_2:+71542895 Msa0927280:five_prime_UTR
TTATCTTTGTTATATTGATT+GGG 0.401990 6_2:+71544019 Msa0927280:five_prime_UTR
AGAAGGAAAATTGGAGAAGA+AGG 0.404624 6_2:+71542954 Msa0927280:five_prime_UTR
TCGTCTACAGACTTCAAATC+TGG 0.404710 6_2:-71543725 None:intergenic
TGTTATCAGACATGCAGAAT+TGG 0.408271 6_2:+71544206 Msa0927280:CDS
GTTTGGTGAAACCCGTATTT+GGG 0.408299 6_2:+71543582 Msa0927280:five_prime_UTR
AGAGGAGAAGAAGAGGAAAC+GGG 0.409530 6_2:+71542919 Msa0927280:five_prime_UTR
AGAGGAGAAGAAGGAAAAGA+AGG 0.414914 6_2:+71542885 Msa0927280:five_prime_UTR
GTGGAGCCCTGTCCCAAATA+CGG 0.417567 6_2:-71543594 None:intergenic
GACAGAGAGGGTCGCGGGAG+AGG 0.423570 6_2:+71542867 None:intergenic
GTTGGAATACAAGTTGTTCC+AGG 0.425290 6_2:+71543328 Msa0927280:five_prime_UTR
TCTTGCAGGGGAGCGGCTGT+GGG 0.427868 6_2:+71543972 Msa0927280:intron
GGTCGCGGGAGAGGAGAAGA+AGG 0.437704 6_2:+71542876 None:intergenic
ATTGTAATTAGGCTACTGAT+TGG 0.457491 6_2:+71543525 Msa0927280:five_prime_UTR
GTGAAACCCGTATTTGGGAC+AGG 0.461048 6_2:+71543587 Msa0927280:five_prime_UTR
TCGAGGGAGAGGAGAAGAAG+AGG 0.474103 6_2:+71542912 Msa0927280:five_prime_UTR
TTGTAATTAGGCTACTGATT+GGG 0.485447 6_2:+71543526 Msa0927280:five_prime_UTR
TGGAGCCCTGTCCCAAATAC+GGG 0.489598 6_2:-71543593 None:intergenic
AGGAAAAGAAGGGAGGTCGA+GGG 0.497212 6_2:+71542896 Msa0927280:five_prime_UTR
TGAGGTGAGATTTGTGGTAG+TGG 0.502293 6_2:-71543000 None:intergenic
GAGGAGAAGAAGGAAAAGAA+GGG 0.503610 6_2:+71542886 Msa0927280:five_prime_UTR
GATTAGGGCAAGGGCAGAAG+TGG 0.505950 6_2:+71543498 Msa0927280:five_prime_UTR
CTGATAACAGAAGGTGGCAC+GGG 0.517200 6_2:-71544192 None:intergenic
GGGACAGGGCTCCACTAAAA+CGG 0.527667 6_2:+71543602 Msa0927280:five_prime_UTR
AAAGTGATTATGGAGGATTA+GGG 0.548430 6_2:+71543483 Msa0927280:five_prime_UTR
ATGATGTGAGGTGAGATTTG+TGG 0.556924 6_2:-71543006 None:intergenic
ACGGGTAACCAAGAGGAAGA+AGG 0.577213 6_2:+71542937 Msa0927280:five_prime_UTR
ATGTGGTTCTTGCAGGGGAG+CGG 0.585315 6_2:+71543965 Msa0927280:intron
TACTGATTGGGATACAAGCT+AGG 0.590571 6_2:+71543538 Msa0927280:five_prime_UTR
GATTATGGAGGATTAGGGCA+AGG 0.593112 6_2:+71543488 Msa0927280:five_prime_UTR
GAGAAGAAGGAAAAGAAGGG+AGG 0.595278 6_2:+71542889 Msa0927280:five_prime_UTR
TCTGATAACAGAAGGTGGCA+CGG 0.608351 6_2:-71544193 None:intergenic
TGAAACCCGTATTTGGGACA+GGG 0.612214 6_2:+71543588 Msa0927280:five_prime_UTR
ATTATGGAGGATTAGGGCAA+GGG 0.614760 6_2:+71543489 Msa0927280:five_prime_UTR
TCTGCATGTCTGATAACAGA+AGG 0.618067 6_2:-71544201 None:intergenic
AATAAAAGAGAAACAACTCA+AGG 0.620396 6_2:-71543998 None:intergenic
TGATAACAGAAGGTGGCACG+GGG 0.636778 6_2:-71544191 None:intergenic
CTGACAGAAAGTGATTATGG+AGG 0.661588 6_2:+71543476 Msa0927280:five_prime_UTR
GCATGTCTGATAACAGAAGG+TGG 0.667716 6_2:-71544198 None:intergenic
AGAGGAAACGGGTAACCAAG+AGG 0.674740 6_2:+71542930 Msa0927280:five_prime_UTR
AAGATGCTGATAATGATGTG+AGG 0.738212 6_2:-71543018 None:intergenic
GATAACAGAAGGTGGCACGG+GGG 0.782659 6_2:-71544190 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTATTCATTCAAT+TGG + chr6_2:71543196-71543215 Msa0927280:intron 15.0%
!!! ATTATCTTTGTTATATTGAT+TGG + chr6_2:71544018-71544037 Msa0927280:five_prime_UTR 15.0%
!!! TTATCTTTGTTATATTGATT+GGG + chr6_2:71544019-71544038 Msa0927280:five_prime_UTR 15.0%
!!! TTTTTTGTTATTCATTCAAT+TGG + chr6_2:71543196-71543215 Msa0927280:intron 15.0%
!!! ATTATCTTTGTTATATTGAT+TGG + chr6_2:71544018-71544037 Msa0927280:five_prime_UTR 15.0%
!!! TTATCTTTGTTATATTGATT+GGG + chr6_2:71544019-71544038 Msa0927280:five_prime_UTR 15.0%
!!! AACTTCAATTTCCAATTTTA+AGG + chr6_2:71543042-71543061 Msa0927280:five_prime_UTR 20.0%
!!! AATTTGATGAATTTAGAGTT+AGG + chr6_2:71543100-71543119 Msa0927280:intron 20.0%
!! ATCAAAACAACTTATATTCA+TGG - chr6_2:71543789-71543808 None:intergenic 20.0%
!! ATCTTATATATGTATGTATG+TGG + chr6_2:71543948-71543967 Msa0927280:intron 20.0%
!! ACTTAAGTCTATTCAAAAAT+TGG - chr6_2:71544275-71544294 None:intergenic 20.0%
!!! AACTTCAATTTCCAATTTTA+AGG + chr6_2:71543042-71543061 Msa0927280:five_prime_UTR 20.0%
!!! AATTTGATGAATTTAGAGTT+AGG + chr6_2:71543100-71543119 Msa0927280:intron 20.0%
!! ATCAAAACAACTTATATTCA+TGG - chr6_2:71543789-71543808 None:intergenic 20.0%
!! ATCTTATATATGTATGTATG+TGG + chr6_2:71543948-71543967 Msa0927280:intron 20.0%
!! ACTTAAGTCTATTCAAAAAT+TGG - chr6_2:71544275-71544294 None:intergenic 20.0%
!!! TTCAATTTCCAATTTTAAGG+TGG + chr6_2:71543045-71543064 Msa0927280:intron 25.0%
!!! TCAATTTCCAATTTTAAGGT+GGG + chr6_2:71543046-71543065 Msa0927280:intron 25.0%
!!! TTTAGAGTTAGGTTTTCATT+TGG + chr6_2:71543111-71543130 Msa0927280:intron 25.0%
!! TTAGAGTTAGGTTTTCATTT+GGG + chr6_2:71543112-71543131 Msa0927280:intron 25.0%
!! AATTGGTAATTAAGTTGTTG+TGG + chr6_2:71543213-71543232 Msa0927280:intron 25.0%
!! ATTGGTAATTAAGTTGTTGT+GGG + chr6_2:71543214-71543233 Msa0927280:intron 25.0%
!!! TGAATTGATGTTTTAGTGTT+TGG + chr6_2:71543244-71543263 Msa0927280:intron 25.0%
!!! GAATTGATGTTTTAGTGTTT+GGG + chr6_2:71543245-71543264 Msa0927280:intron 25.0%
! TGGTAACTTAAATATAGCAT+TGG - chr6_2:71543378-71543397 None:intergenic 25.0%
!! GTGAACTGATAAACTATTTT+GGG + chr6_2:71543410-71543429 Msa0927280:intron 25.0%
! GAAGTGGATAAATTGTAATT+AGG + chr6_2:71543514-71543533 Msa0927280:five_prime_UTR 25.0%
! CCTATAATAACTATCAAGTT+AGG - chr6_2:71543845-71543864 None:intergenic 25.0%
!! CCTAACTTGATAGTTATTAT+AGG + chr6_2:71543842-71543861 Msa0927280:intron 25.0%
! TGAATATGATGAAGTCAATA+AGG + chr6_2:71543922-71543941 Msa0927280:intron 25.0%
! AATAAAAGAGAAACAACTCA+AGG - chr6_2:71544001-71544020 None:intergenic 25.0%
!! TGTTATATTGATTGGGAATA+TGG + chr6_2:71544026-71544045 Msa0927280:exon 25.0%
! CAAAACAAAACAAAAAACTG+TGG - chr6_2:71544090-71544109 None:intergenic 25.0%
!!! TTCAATTTCCAATTTTAAGG+TGG + chr6_2:71543045-71543064 Msa0927280:intron 25.0%
!!! TCAATTTCCAATTTTAAGGT+GGG + chr6_2:71543046-71543065 Msa0927280:intron 25.0%
!!! TTTAGAGTTAGGTTTTCATT+TGG + chr6_2:71543111-71543130 Msa0927280:intron 25.0%
!! TTAGAGTTAGGTTTTCATTT+GGG + chr6_2:71543112-71543131 Msa0927280:intron 25.0%
!! AATTGGTAATTAAGTTGTTG+TGG + chr6_2:71543213-71543232 Msa0927280:intron 25.0%
!! ATTGGTAATTAAGTTGTTGT+GGG + chr6_2:71543214-71543233 Msa0927280:intron 25.0%
!!! TGAATTGATGTTTTAGTGTT+TGG + chr6_2:71543244-71543263 Msa0927280:intron 25.0%
!!! GAATTGATGTTTTAGTGTTT+GGG + chr6_2:71543245-71543264 Msa0927280:intron 25.0%
! TGGTAACTTAAATATAGCAT+TGG - chr6_2:71543378-71543397 None:intergenic 25.0%
!! GTGAACTGATAAACTATTTT+GGG + chr6_2:71543410-71543429 Msa0927280:intron 25.0%
! GAAGTGGATAAATTGTAATT+AGG + chr6_2:71543514-71543533 Msa0927280:five_prime_UTR 25.0%
! CCTATAATAACTATCAAGTT+AGG - chr6_2:71543845-71543864 None:intergenic 25.0%
!! CCTAACTTGATAGTTATTAT+AGG + chr6_2:71543842-71543861 Msa0927280:intron 25.0%
! TGAATATGATGAAGTCAATA+AGG + chr6_2:71543922-71543941 Msa0927280:intron 25.0%
! AATAAAAGAGAAACAACTCA+AGG - chr6_2:71544001-71544020 None:intergenic 25.0%
!! TGTTATATTGATTGGGAATA+TGG + chr6_2:71544026-71544045 Msa0927280:exon 25.0%
! CAAAACAAAACAAAAAACTG+TGG - chr6_2:71544090-71544109 None:intergenic 25.0%
TCAAATTTCTACATGCATGA+GGG - chr6_2:71543087-71543106 None:intergenic 30.0%
ATCAAATTTCTACATGCATG+AGG - chr6_2:71543088-71543107 None:intergenic 30.0%
! TAGAGTTAGGTTTTCATTTG+GGG + chr6_2:71543113-71543132 Msa0927280:intron 30.0%
! TTGGTAATTAAGTTGTTGTG+GGG + chr6_2:71543215-71543234 Msa0927280:intron 30.0%
TAAGTTACCATATGAGTAGT+TGG + chr6_2:71543388-71543407 Msa0927280:intron 30.0%
! GGTGAACTGATAAACTATTT+TGG + chr6_2:71543409-71543428 Msa0927280:intron 30.0%
AAAGTGATTATGGAGGATTA+GGG + chr6_2:71543483-71543502 Msa0927280:five_prime_UTR 30.0%
ATTGTAATTAGGCTACTGAT+TGG + chr6_2:71543525-71543544 Msa0927280:five_prime_UTR 30.0%
! TTGTAATTAGGCTACTGATT+GGG + chr6_2:71543526-71543545 Msa0927280:five_prime_UTR 30.0%
! TCAAGTGATTTTTGATGCTA+TGG + chr6_2:71543652-71543671 Msa0927280:intron 30.0%
!!! TGTGCTGATAAATTTTTTGC+AGG + chr6_2:71543808-71543827 Msa0927280:five_prime_UTR 30.0%
TCTATTCAAAAATTGGTCAG+GGG - chr6_2:71544268-71544287 None:intergenic 30.0%
GTCTATTCAAAAATTGGTCA+GGG - chr6_2:71544269-71544288 None:intergenic 30.0%
AGTCTATTCAAAAATTGGTC+AGG - chr6_2:71544270-71544289 None:intergenic 30.0%
TCAAATTTCTACATGCATGA+GGG - chr6_2:71543087-71543106 None:intergenic 30.0%
ATCAAATTTCTACATGCATG+AGG - chr6_2:71543088-71543107 None:intergenic 30.0%
! TAGAGTTAGGTTTTCATTTG+GGG + chr6_2:71543113-71543132 Msa0927280:intron 30.0%
! TTGGTAATTAAGTTGTTGTG+GGG + chr6_2:71543215-71543234 Msa0927280:intron 30.0%
TAAGTTACCATATGAGTAGT+TGG + chr6_2:71543388-71543407 Msa0927280:intron 30.0%
! GGTGAACTGATAAACTATTT+TGG + chr6_2:71543409-71543428 Msa0927280:intron 30.0%
AAAGTGATTATGGAGGATTA+GGG + chr6_2:71543483-71543502 Msa0927280:five_prime_UTR 30.0%
ATTGTAATTAGGCTACTGAT+TGG + chr6_2:71543525-71543544 Msa0927280:five_prime_UTR 30.0%
! TTGTAATTAGGCTACTGATT+GGG + chr6_2:71543526-71543545 Msa0927280:five_prime_UTR 30.0%
! TCAAGTGATTTTTGATGCTA+TGG + chr6_2:71543652-71543671 Msa0927280:intron 30.0%
!!! TGTGCTGATAAATTTTTTGC+AGG + chr6_2:71543808-71543827 Msa0927280:five_prime_UTR 30.0%
TCTATTCAAAAATTGGTCAG+GGG - chr6_2:71544268-71544287 None:intergenic 30.0%
GTCTATTCAAAAATTGGTCA+GGG - chr6_2:71544269-71544288 None:intergenic 30.0%
AGTCTATTCAAAAATTGGTC+AGG - chr6_2:71544270-71544289 None:intergenic 30.0%
AGAAGGAAAATTGGAGAAGA+AGG + chr6_2:71542954-71542973 Msa0927280:five_prime_UTR 35.0%
! AAGATGCTGATAATGATGTG+AGG - chr6_2:71543021-71543040 None:intergenic 35.0%
! AGAGTTAGGTTTTCATTTGG+GGG + chr6_2:71543114-71543133 Msa0927280:intron 35.0%
! TTGGGGGAAAAGAGAATTTT+AGG + chr6_2:71543130-71543149 Msa0927280:intron 35.0%
! TGGGGGAAAAGAGAATTTTA+GGG + chr6_2:71543131-71543150 Msa0927280:intron 35.0%
! TGTTTTGCTGAAGCTTAAGT+TGG + chr6_2:71543310-71543329 Msa0927280:five_prime_UTR 35.0%
AATACAAGTTGTTCCAGGTA+TGG + chr6_2:71543333-71543352 Msa0927280:intron 35.0%
AAGCTGACAGAAAGTGATTA+TGG + chr6_2:71543473-71543492 Msa0927280:five_prime_UTR 35.0%
GAAAGTGATTATGGAGGATT+AGG + chr6_2:71543482-71543501 Msa0927280:five_prime_UTR 35.0%
!! AGCTAGGAATAAGCTGTTTT+TGG + chr6_2:71543554-71543573 Msa0927280:five_prime_UTR 35.0%
!!! AGCTGTTTTTGGAAGAAGTT+TGG + chr6_2:71543565-71543584 Msa0927280:five_prime_UTR 35.0%
TCACTTGATTCCAAGTTACA+GGG - chr6_2:71543640-71543659 None:intergenic 35.0%
ATCACTTGATTCCAAGTTAC+AGG - chr6_2:71543641-71543660 None:intergenic 35.0%
! ATGGTTAGCTAACGAAATTC+CGG + chr6_2:71543671-71543690 Msa0927280:intron 35.0%
!!! GGTACCAGTTTCATTGATTT+TGG + chr6_2:71543701-71543720 Msa0927280:intron 35.0%
TGGACCAAAATCAATGAAAC+TGG - chr6_2:71543708-71543727 None:intergenic 35.0%
!!! TCTGTAGACGATTTTGAAGT+TGG + chr6_2:71543737-71543756 Msa0927280:five_prime_UTR 35.0%
!!! CTGTAGACGATTTTGAAGTT+GGG + chr6_2:71543738-71543757 Msa0927280:five_prime_UTR 35.0%
!!! TGTAGACGATTTTGAAGTTG+GGG + chr6_2:71543739-71543758 Msa0927280:five_prime_UTR 35.0%
AATGATGTAAGCATGTCTGA+TGG + chr6_2:71543867-71543886 Msa0927280:intron 35.0%
ATTACTACTTCACTCATGCA+AGG - chr6_2:71543898-71543917 None:intergenic 35.0%
TGCATGAGTGAAGTAGTAAT+AGG + chr6_2:71543898-71543917 Msa0927280:intron 35.0%
GCATGAGTGAAGTAGTAATA+GGG + chr6_2:71543899-71543918 Msa0927280:intron 35.0%
!! TTGGGAATATGGTGTTTTTG+AGG + chr6_2:71544037-71544056 Msa0927280:exon 35.0%
!!! GTGTTTTTGAGGTAAGCAAT+AGG + chr6_2:71544048-71544067 Msa0927280:intron 35.0%
AACTGTGGAGATTCTATCAA+GGG - chr6_2:71544075-71544094 None:intergenic 35.0%
AAACTGTGGAGATTCTATCA+AGG - chr6_2:71544076-71544095 None:intergenic 35.0%
! TACTGCTACATTTTACGTTG+CGG + chr6_2:71544149-71544168 Msa0927280:CDS 35.0%
TGTTATCAGACATGCAGAAT+TGG + chr6_2:71544206-71544225 Msa0927280:CDS 35.0%
! CTTAAGTTCAAGTTCATTGC+AGG + chr6_2:71544288-71544307 Msa0927280:three_prime_UTR 35.0%
AGAAGGAAAATTGGAGAAGA+AGG + chr6_2:71542954-71542973 Msa0927280:five_prime_UTR 35.0%
! AAGATGCTGATAATGATGTG+AGG - chr6_2:71543021-71543040 None:intergenic 35.0%
! AGAGTTAGGTTTTCATTTGG+GGG + chr6_2:71543114-71543133 Msa0927280:intron 35.0%
! TTGGGGGAAAAGAGAATTTT+AGG + chr6_2:71543130-71543149 Msa0927280:intron 35.0%
! TGGGGGAAAAGAGAATTTTA+GGG + chr6_2:71543131-71543150 Msa0927280:intron 35.0%
! TGTTTTGCTGAAGCTTAAGT+TGG + chr6_2:71543310-71543329 Msa0927280:five_prime_UTR 35.0%
AATACAAGTTGTTCCAGGTA+TGG + chr6_2:71543333-71543352 Msa0927280:intron 35.0%
AAGCTGACAGAAAGTGATTA+TGG + chr6_2:71543473-71543492 Msa0927280:five_prime_UTR 35.0%
GAAAGTGATTATGGAGGATT+AGG + chr6_2:71543482-71543501 Msa0927280:five_prime_UTR 35.0%
!! AGCTAGGAATAAGCTGTTTT+TGG + chr6_2:71543554-71543573 Msa0927280:five_prime_UTR 35.0%
!!! AGCTGTTTTTGGAAGAAGTT+TGG + chr6_2:71543565-71543584 Msa0927280:five_prime_UTR 35.0%
TCACTTGATTCCAAGTTACA+GGG - chr6_2:71543640-71543659 None:intergenic 35.0%
ATCACTTGATTCCAAGTTAC+AGG - chr6_2:71543641-71543660 None:intergenic 35.0%
! ATGGTTAGCTAACGAAATTC+CGG + chr6_2:71543671-71543690 Msa0927280:intron 35.0%
!!! GGTACCAGTTTCATTGATTT+TGG + chr6_2:71543701-71543720 Msa0927280:intron 35.0%
TGGACCAAAATCAATGAAAC+TGG - chr6_2:71543708-71543727 None:intergenic 35.0%
!!! TCTGTAGACGATTTTGAAGT+TGG + chr6_2:71543737-71543756 Msa0927280:five_prime_UTR 35.0%
!!! CTGTAGACGATTTTGAAGTT+GGG + chr6_2:71543738-71543757 Msa0927280:five_prime_UTR 35.0%
!!! TGTAGACGATTTTGAAGTTG+GGG + chr6_2:71543739-71543758 Msa0927280:five_prime_UTR 35.0%
AATGATGTAAGCATGTCTGA+TGG + chr6_2:71543867-71543886 Msa0927280:intron 35.0%
ATTACTACTTCACTCATGCA+AGG - chr6_2:71543898-71543917 None:intergenic 35.0%
TGCATGAGTGAAGTAGTAAT+AGG + chr6_2:71543898-71543917 Msa0927280:intron 35.0%
GCATGAGTGAAGTAGTAATA+GGG + chr6_2:71543899-71543918 Msa0927280:intron 35.0%
!! TTGGGAATATGGTGTTTTTG+AGG + chr6_2:71544037-71544056 Msa0927280:exon 35.0%
!!! GTGTTTTTGAGGTAAGCAAT+AGG + chr6_2:71544048-71544067 Msa0927280:intron 35.0%
AACTGTGGAGATTCTATCAA+GGG - chr6_2:71544075-71544094 None:intergenic 35.0%
AAACTGTGGAGATTCTATCA+AGG - chr6_2:71544076-71544095 None:intergenic 35.0%
! TACTGCTACATTTTACGTTG+CGG + chr6_2:71544149-71544168 Msa0927280:CDS 35.0%
TGTTATCAGACATGCAGAAT+TGG + chr6_2:71544206-71544225 Msa0927280:CDS 35.0%
! CTTAAGTTCAAGTTCATTGC+AGG + chr6_2:71544288-71544307 Msa0927280:three_prime_UTR 35.0%
AGAGGAGAAGAAGGAAAAGA+AGG + chr6_2:71542885-71542904 Msa0927280:five_prime_UTR 40.0%
GAGGAGAAGAAGGAAAAGAA+GGG + chr6_2:71542886-71542905 Msa0927280:five_prime_UTR 40.0%
! CCAATTTTCCTTCTTCCTCT+TGG - chr6_2:71542948-71542967 None:intergenic 40.0%
CCAAGAGGAAGAAGGAAAAT+TGG + chr6_2:71542945-71542964 Msa0927280:five_prime_UTR 40.0%
ATGATGTGAGGTGAGATTTG+TGG - chr6_2:71543009-71543028 None:intergenic 40.0%
AGGGAAACCCACCTTAAAAT+TGG - chr6_2:71543056-71543075 None:intergenic 40.0%
ATGCATGAGGGAAAGGAAAA+GGG - chr6_2:71543075-71543094 None:intergenic 40.0%
TTTCTACATGCATGAGGGAA+AGG - chr6_2:71543082-71543101 None:intergenic 40.0%
GTTGGAATACAAGTTGTTCC+AGG + chr6_2:71543328-71543347 Msa0927280:five_prime_UTR 40.0%
AAAGAACACACAACCATACC+TGG - chr6_2:71543349-71543368 None:intergenic 40.0%
CAGTTCACCAACTACTCATA+TGG - chr6_2:71543398-71543417 None:intergenic 40.0%
CTGACAGAAAGTGATTATGG+AGG + chr6_2:71543476-71543495 Msa0927280:five_prime_UTR 40.0%
ATTATGGAGGATTAGGGCAA+GGG + chr6_2:71543489-71543508 Msa0927280:five_prime_UTR 40.0%
! TACTGATTGGGATACAAGCT+AGG + chr6_2:71543538-71543557 Msa0927280:five_prime_UTR 40.0%
! AGTTTGGTGAAACCCGTATT+TGG + chr6_2:71543581-71543600 Msa0927280:five_prime_UTR 40.0%
! GTTTGGTGAAACCCGTATTT+GGG + chr6_2:71543582-71543601 Msa0927280:five_prime_UTR 40.0%
! GTGAGTTCTTACCGTTTTAG+TGG - chr6_2:71543616-71543635 None:intergenic 40.0%
! TAACGAAATTCCGGACGTTT+TGG + chr6_2:71543680-71543699 Msa0927280:intron 40.0%
TCGTCTACAGACTTCAAATC+TGG - chr6_2:71543728-71543747 None:intergenic 40.0%
!!! GTAGACGATTTTGAAGTTGG+GGG + chr6_2:71543740-71543759 Msa0927280:five_prime_UTR 40.0%
TGTATGTATGTGGTTCTTGC+AGG + chr6_2:71543958-71543977 Msa0927280:intron 40.0%
GTATGTATGTGGTTCTTGCA+GGG + chr6_2:71543959-71543978 Msa0927280:intron 40.0%
TATGTATGTGGTTCTTGCAG+GGG + chr6_2:71543960-71543979 Msa0927280:intron 40.0%
! TTTTACGTTGCGGCTAGATA+TGG + chr6_2:71544159-71544178 Msa0927280:CDS 40.0%
TCTGCATGTCTGATAACAGA+AGG - chr6_2:71544204-71544223 None:intergenic 40.0%
AGAGGAGAAGAAGGAAAAGA+AGG + chr6_2:71542885-71542904 Msa0927280:five_prime_UTR 40.0%
GAGGAGAAGAAGGAAAAGAA+GGG + chr6_2:71542886-71542905 Msa0927280:five_prime_UTR 40.0%
! CCAATTTTCCTTCTTCCTCT+TGG - chr6_2:71542948-71542967 None:intergenic 40.0%
CCAAGAGGAAGAAGGAAAAT+TGG + chr6_2:71542945-71542964 Msa0927280:five_prime_UTR 40.0%
ATGATGTGAGGTGAGATTTG+TGG - chr6_2:71543009-71543028 None:intergenic 40.0%
AGGGAAACCCACCTTAAAAT+TGG - chr6_2:71543056-71543075 None:intergenic 40.0%
ATGCATGAGGGAAAGGAAAA+GGG - chr6_2:71543075-71543094 None:intergenic 40.0%
TTTCTACATGCATGAGGGAA+AGG - chr6_2:71543082-71543101 None:intergenic 40.0%
GTTGGAATACAAGTTGTTCC+AGG + chr6_2:71543328-71543347 Msa0927280:five_prime_UTR 40.0%
AAAGAACACACAACCATACC+TGG - chr6_2:71543349-71543368 None:intergenic 40.0%
CAGTTCACCAACTACTCATA+TGG - chr6_2:71543398-71543417 None:intergenic 40.0%
CTGACAGAAAGTGATTATGG+AGG + chr6_2:71543476-71543495 Msa0927280:five_prime_UTR 40.0%
ATTATGGAGGATTAGGGCAA+GGG + chr6_2:71543489-71543508 Msa0927280:five_prime_UTR 40.0%
! TACTGATTGGGATACAAGCT+AGG + chr6_2:71543538-71543557 Msa0927280:five_prime_UTR 40.0%
! AGTTTGGTGAAACCCGTATT+TGG + chr6_2:71543581-71543600 Msa0927280:five_prime_UTR 40.0%
! GTTTGGTGAAACCCGTATTT+GGG + chr6_2:71543582-71543601 Msa0927280:five_prime_UTR 40.0%
! GTGAGTTCTTACCGTTTTAG+TGG - chr6_2:71543616-71543635 None:intergenic 40.0%
! TAACGAAATTCCGGACGTTT+TGG + chr6_2:71543680-71543699 Msa0927280:intron 40.0%
TCGTCTACAGACTTCAAATC+TGG - chr6_2:71543728-71543747 None:intergenic 40.0%
!!! GTAGACGATTTTGAAGTTGG+GGG + chr6_2:71543740-71543759 Msa0927280:five_prime_UTR 40.0%
TGTATGTATGTGGTTCTTGC+AGG + chr6_2:71543958-71543977 Msa0927280:intron 40.0%
GTATGTATGTGGTTCTTGCA+GGG + chr6_2:71543959-71543978 Msa0927280:intron 40.0%
TATGTATGTGGTTCTTGCAG+GGG + chr6_2:71543960-71543979 Msa0927280:intron 40.0%
! TTTTACGTTGCGGCTAGATA+TGG + chr6_2:71544159-71544178 Msa0927280:CDS 40.0%
TCTGCATGTCTGATAACAGA+AGG - chr6_2:71544204-71544223 None:intergenic 40.0%
GAGAAGAAGGAAAAGAAGGG+AGG + chr6_2:71542889-71542908 Msa0927280:five_prime_UTR 45.0%
GAGAGGAGAAGAAGAGGAAA+CGG + chr6_2:71542918-71542937 Msa0927280:five_prime_UTR 45.0%
AGAGGAGAAGAAGAGGAAAC+GGG + chr6_2:71542919-71542938 Msa0927280:five_prime_UTR 45.0%
TGAGGTGAGATTTGTGGTAG+TGG - chr6_2:71543003-71543022 None:intergenic 45.0%
CATGCATGAGGGAAAGGAAA+AGG - chr6_2:71543076-71543095 None:intergenic 45.0%
GATTATGGAGGATTAGGGCA+AGG + chr6_2:71543488-71543507 Msa0927280:five_prime_UTR 45.0%
! TGAAACCCGTATTTGGGACA+GGG + chr6_2:71543588-71543607 Msa0927280:five_prime_UTR 45.0%
AGAACTCACTCCCTGTAACT+TGG + chr6_2:71543627-71543646 Msa0927280:intron 45.0%
GAAACTGGTACCAAAACGTC+CGG - chr6_2:71543693-71543712 None:intergenic 45.0%
TCTGATAACAGAAGGTGGCA+CGG - chr6_2:71544196-71544215 None:intergenic 45.0%
GCATGTCTGATAACAGAAGG+TGG - chr6_2:71544201-71544220 None:intergenic 45.0%
GAGAAGAAGGAAAAGAAGGG+AGG + chr6_2:71542889-71542908 Msa0927280:five_prime_UTR 45.0%
GAGAGGAGAAGAAGAGGAAA+CGG + chr6_2:71542918-71542937 Msa0927280:five_prime_UTR 45.0%
AGAGGAGAAGAAGAGGAAAC+GGG + chr6_2:71542919-71542938 Msa0927280:five_prime_UTR 45.0%
TGAGGTGAGATTTGTGGTAG+TGG - chr6_2:71543003-71543022 None:intergenic 45.0%
CATGCATGAGGGAAAGGAAA+AGG - chr6_2:71543076-71543095 None:intergenic 45.0%
GATTATGGAGGATTAGGGCA+AGG + chr6_2:71543488-71543507 Msa0927280:five_prime_UTR 45.0%
! TGAAACCCGTATTTGGGACA+GGG + chr6_2:71543588-71543607 Msa0927280:five_prime_UTR 45.0%
AGAACTCACTCCCTGTAACT+TGG + chr6_2:71543627-71543646 Msa0927280:intron 45.0%
GAAACTGGTACCAAAACGTC+CGG - chr6_2:71543693-71543712 None:intergenic 45.0%
TCTGATAACAGAAGGTGGCA+CGG - chr6_2:71544196-71544215 None:intergenic 45.0%
GCATGTCTGATAACAGAAGG+TGG - chr6_2:71544201-71544220 None:intergenic 45.0%
AAGGAAAAGAAGGGAGGTCG+AGG + chr6_2:71542895-71542914 Msa0927280:five_prime_UTR 50.0%
AGGAAAAGAAGGGAGGTCGA+GGG + chr6_2:71542896-71542915 Msa0927280:five_prime_UTR 50.0%
AGAGGAAACGGGTAACCAAG+AGG + chr6_2:71542930-71542949 Msa0927280:five_prime_UTR 50.0%
ACGGGTAACCAAGAGGAAGA+AGG + chr6_2:71542937-71542956 Msa0927280:five_prime_UTR 50.0%
! GTGAAACCCGTATTTGGGAC+AGG + chr6_2:71543587-71543606 Msa0927280:five_prime_UTR 50.0%
TTGAAGTTGGGGGCAGAAGT+AGG + chr6_2:71543750-71543769 Msa0927280:five_prime_UTR 50.0%
TGATAACAGAAGGTGGCACG+GGG - chr6_2:71544194-71544213 None:intergenic 50.0%
CTGATAACAGAAGGTGGCAC+GGG - chr6_2:71544195-71544214 None:intergenic 50.0%
AAGGAAAAGAAGGGAGGTCG+AGG + chr6_2:71542895-71542914 Msa0927280:five_prime_UTR 50.0%
AGGAAAAGAAGGGAGGTCGA+GGG + chr6_2:71542896-71542915 Msa0927280:five_prime_UTR 50.0%
AGAGGAAACGGGTAACCAAG+AGG + chr6_2:71542930-71542949 Msa0927280:five_prime_UTR 50.0%
ACGGGTAACCAAGAGGAAGA+AGG + chr6_2:71542937-71542956 Msa0927280:five_prime_UTR 50.0%
! GTGAAACCCGTATTTGGGAC+AGG + chr6_2:71543587-71543606 Msa0927280:five_prime_UTR 50.0%
TTGAAGTTGGGGGCAGAAGT+AGG + chr6_2:71543750-71543769 Msa0927280:five_prime_UTR 50.0%
TGATAACAGAAGGTGGCACG+GGG - chr6_2:71544194-71544213 None:intergenic 50.0%
CTGATAACAGAAGGTGGCAC+GGG - chr6_2:71544195-71544214 None:intergenic 50.0%
TCGAGGGAGAGGAGAAGAAG+AGG + chr6_2:71542912-71542931 Msa0927280:five_prime_UTR 55.0%
GATTAGGGCAAGGGCAGAAG+TGG + chr6_2:71543498-71543517 Msa0927280:five_prime_UTR 55.0%
TGGAGCCCTGTCCCAAATAC+GGG - chr6_2:71543596-71543615 None:intergenic 55.0%
GTGGAGCCCTGTCCCAAATA+CGG - chr6_2:71543597-71543616 None:intergenic 55.0%
GGGACAGGGCTCCACTAAAA+CGG + chr6_2:71543602-71543621 Msa0927280:five_prime_UTR 55.0%
ATGTGGTTCTTGCAGGGGAG+CGG + chr6_2:71543965-71543984 Msa0927280:intron 55.0%
GATAACAGAAGGTGGCACGG+GGG - chr6_2:71544193-71544212 None:intergenic 55.0%
TCGAGGGAGAGGAGAAGAAG+AGG + chr6_2:71542912-71542931 Msa0927280:five_prime_UTR 55.0%
GATTAGGGCAAGGGCAGAAG+TGG + chr6_2:71543498-71543517 Msa0927280:five_prime_UTR 55.0%
TGGAGCCCTGTCCCAAATAC+GGG - chr6_2:71543596-71543615 None:intergenic 55.0%
GTGGAGCCCTGTCCCAAATA+CGG - chr6_2:71543597-71543616 None:intergenic 55.0%
GGGACAGGGCTCCACTAAAA+CGG + chr6_2:71543602-71543621 Msa0927280:five_prime_UTR 55.0%
ATGTGGTTCTTGCAGGGGAG+CGG + chr6_2:71543965-71543984 Msa0927280:intron 55.0%
GATAACAGAAGGTGGCACGG+GGG - chr6_2:71544193-71544212 None:intergenic 55.0%
AAGAAGGGAGGTCGAGGGAG+AGG + chr6_2:71542901-71542920 Msa0927280:five_prime_UTR 60.0%
AAGAAGGGAGGTCGAGGGAG+AGG + chr6_2:71542901-71542920 Msa0927280:five_prime_UTR 60.0%
TTCTTGCAGGGGAGCGGCTG+TGG + chr6_2:71543971-71543990 Msa0927280:intron 65.0%
TCTTGCAGGGGAGCGGCTGT+GGG + chr6_2:71543972-71543991 Msa0927280:intron 65.0%
TTCTTGCAGGGGAGCGGCTG+TGG + chr6_2:71543971-71543990 Msa0927280:intron 65.0%
TCTTGCAGGGGAGCGGCTGT+GGG + chr6_2:71543972-71543991 Msa0927280:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 71542880 71544314 71542880 ID=Msa0927280;Name=Msa0927280
chr6_2 mRNA 71542880 71544314 71542880 ID=Msa0927280-mRNA-1;Parent=Msa0927280;Name=Msa0927280-mRNA-1;_AED=0.54;_eAED=0.54;_QI=642|0.8|0.83|0.83|0.2|0.33|6|62|39
chr6_2 exon 71542880 71543063 71542880 ID=Msa0927280-mRNA-1:exon:11888;Parent=Msa0927280-mRNA-1
chr6_2 exon 71543260 71543349 71543260 ID=Msa0927280-mRNA-1:exon:11889;Parent=Msa0927280-mRNA-1
chr6_2 exon 71543442 71543623 71543442 ID=Msa0927280-mRNA-1:exon:11890;Parent=Msa0927280-mRNA-1
chr6_2 exon 71543709 71543829 71543709 ID=Msa0927280-mRNA-1:exon:11891;Parent=Msa0927280-mRNA-1
chr6_2 exon 71543980 71544058 71543980 ID=Msa0927280-mRNA-1:exon:11892;Parent=Msa0927280-mRNA-1
chr6_2 exon 71544147 71544314 71544147 ID=Msa0927280-mRNA-1:exon:11893;Parent=Msa0927280-mRNA-1
chr6_2 five_prime_UTR 71542880 71543063 71542880 ID=Msa0927280-mRNA-1:five_prime_utr;Parent=Msa0927280-mRNA-1
chr6_2 five_prime_UTR 71543260 71543349 71543260 ID=Msa0927280-mRNA-1:five_prime_utr;Parent=Msa0927280-mRNA-1
chr6_2 five_prime_UTR 71543442 71543623 71543442 ID=Msa0927280-mRNA-1:five_prime_utr;Parent=Msa0927280-mRNA-1
chr6_2 five_prime_UTR 71543709 71543829 71543709 ID=Msa0927280-mRNA-1:five_prime_utr;Parent=Msa0927280-mRNA-1
chr6_2 five_prime_UTR 71543980 71544044 71543980 ID=Msa0927280-mRNA-1:five_prime_utr;Parent=Msa0927280-mRNA-1
chr6_2 CDS 71544045 71544058 71544045 ID=Msa0927280-mRNA-1:cds;Parent=Msa0927280-mRNA-1
chr6_2 CDS 71544147 71544252 71544147 ID=Msa0927280-mRNA-1:cds;Parent=Msa0927280-mRNA-1
chr6_2 three_prime_UTR 71544253 71544314 71544253 ID=Msa0927280-mRNA-1:three_prime_utr;Parent=Msa0927280-mRNA-1
Gene Sequence

>Msa0927280

ATGGTGTTTTTGAGTCTACTGCTACATTTTACGTTGCGGCTAGATATGGAAGTGCAACCCCCGTGCCACCTTCTGTTATCAGACATGCAGAATTGGCGTTTTGCATTGTCTTATTTATGA
Protein sequence

>Msa0927280

MVFLSLLLHFTLRLDMEVQPPCHLLLSDMQNWRFALSYL*