AlfalfaGEDB | Msa0452960

M. sativa cultivar zhongmu-4 / Msa0452960

KEGG: -
GO: -
PFAM: PF01190.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452960	XP_003600045.1	98.639	147	2	0	1	147	1	147	6.45e-104	303

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452960	G7IXJ5	98.639	147	2	0	1	147	1	147	3.08e-104	303

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa0452960	-0.814234	1.709031e-51	-8.615850e-47
Msa0006390	Msa0452960	0.810188	1.304985e-50	-8.615850e-47
Msa0008340	Msa0452960	0.833642	4.743013e-56	-8.615850e-47
Msa0009760	Msa0452960	0.817706	2.868699e-52	-8.615850e-47
Msa0015120	Msa0452960	0.805033	1.623108e-49	-8.615850e-47
Msa0017510	Msa0452960	0.825331	4.969603e-54	-8.615850e-47
Msa0020930	Msa0452960	0.805060	1.601591e-49	-8.615850e-47
Msa0031340	Msa0452960	0.804059	2.590614e-49	-8.615850e-47
Msa0032370	Msa0452960	0.801272	9.735019e-49	-8.615850e-47
Msa0032840	Msa0452960	-0.802978	4.339640e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452960	MtrunA17_Chr3g0098611	98.639	147	2	0	1	147	1	147	5.93e-108	303

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452960	AT5G54855.1	67.123	146	48	0	1	146	1	146	6.75e-73	215

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Find 46 sgRNAs with CRISPR-Local

Find 548 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGAAGCAGAAGATGATAG+TGG	0.473190	3_3:+42859375	Msa0452960:CDS
AATGCCTGAGAGTGATCGTT+GGG	0.564183	3_3:+42862867	Msa0452960:CDS
ACAGCATGTGAATACCCCGA+TGG	0.710104	3_3:-42862982	None:intergenic
AGAGTTGTTTGTGATGTTTG+TGG	0.528736	3_3:+42859466	Msa0452960:CDS
AGGCATTTACAGATGCTAAG+GGG	0.710498	3_3:+42862824	Msa0452960:CDS
AGTGATTCAGCGAGTGTCGG+TGG	0.585112	3_3:+42859255	None:intergenic
ATATATCAGATGCAGTATGT+TGG	0.578522	3_3:-42863036	None:intergenic
ATGGTATTGCCATCTTAGAT+AGG	0.508832	3_3:+42863246	Msa0452960:three_prime_UTR
ATGTTGGAACATTGACAGGT+TGG	0.603546	3_3:-42863020	None:intergenic
ATGTTTATGCAGGAAACAAT+AGG	0.408789	3_3:-42863370	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATCTTTGTCTCTCTCTAAT+TGG	+	chr3_3:42859528-42859547	Msa0452960:intron	30.0%
ATATCCAACACAAACTCAAT+TGG	-	chr3_3:42859724-42859743	None:intergenic	30.0%
CGATTTCATTTACGAATAAG+TGG	+	chr3_3:42859795-42859814	Msa0452960:intron	30.0%
GATAGCAATCTAATAAAGTG+AGG	-	chr3_3:42859940-42859959	None:intergenic	30.0%
AAACACTTATGTGTATAGCA+AGG	-	chr3_3:42860057-42860076	None:intergenic	30.0%
ATAAGCAGATTCTTGTTAGA+AGG	+	chr3_3:42860100-42860119	Msa0452960:intron	30.0%
TAAGCAGATTCTTGTTAGAA+GGG	+	chr3_3:42860101-42860120	Msa0452960:intron	30.0%
AGTTGATTGCAAGAACATTA+GGG	-	chr3_3:42860141-42860160	None:intergenic	30.0%
TGTTCTTGCAATCAACTTTA+TGG	+	chr3_3:42860144-42860163	Msa0452960:intron	30.0%
GTTCTTGCAATCAACTTTAT+GGG	+	chr3_3:42860145-42860164	Msa0452960:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	42859266	42863383	42859266	ID=Msa0452960;Name=Msa0452960
chr3_3	mRNA	42859266	42863383	42859266	ID=Msa0452960-mRNA-1;Parent=Msa0452960;Name=Msa0452960-mRNA-1;_AED=0.20;_eAED=0.20;_QI=101\|1\|1\|1\|1\|1\|2\|330\|147
chr3_3	exon	42859266	42859526	42859266	ID=Msa0452960-mRNA-1:exon:7728;Parent=Msa0452960-mRNA-1
chr3_3	exon	42862770	42863383	42862770	ID=Msa0452960-mRNA-1:exon:7729;Parent=Msa0452960-mRNA-1
chr3_3	five_prime_UTR	42859266	42859366	42859266	ID=Msa0452960-mRNA-1:five_prime_utr;Parent=Msa0452960-mRNA-1
chr3_3	CDS	42859367	42859526	42859367	ID=Msa0452960-mRNA-1:cds;Parent=Msa0452960-mRNA-1
chr3_3	CDS	42862770	42863053	42862770	ID=Msa0452960-mRNA-1:cds;Parent=Msa0452960-mRNA-1
chr3_3	three_prime_UTR	42863054	42863383	42863054	ID=Msa0452960-mRNA-1:three_prime_utr;Parent=Msa0452960-mRNA-1

Gene Sequence

>Msa0452960

ATGGAAGGAATGAAGCAGAAGATGATAGTGGAATTGATTGTGTTGTTGATGGTGGTGTTTGTTTTGAGTGTAAGTGGTTGGACTGGTGAAATTCGTGGAAGAGTTGTTTGTGATGTTTGTGGTGATTCTTCTCTTGGTCCTGAAGACCATGTTCTTGAAGGTGCTGAAGTTGCTGTTCTTTGCATAACCAAGTCCGGCGAAGTTCTAAATTATCAGGCATTTACAGATGCTAAGGGGATATACACAGTAGCTGAGACAATGCCTGAGAGTGATCGTTGGGATGCATGTCTTGCTCGACCAATCAGTAGTTTCCATGAGCACTGCACTCGTCTTGGTGGTGGTAGTTCGGGGATTAAATTCAGTTACAATCATCCATCGGGGTATTCACATGCTGTCAGAACCTTTGTTTACCAACCTGTCAATGTTCCAACATACTGCATCTGA

Protein sequence

>Msa0452960

MEGMKQKMIVELIVLLMVVFVLSVSGWTGEIRGRVVCDVCGDSSLGPEDHVLEGAEVAVLCITKSGEVLNYQAFTDAKGIYTVAETMPESDRWDACLARPISSFHEHCTRLGGGSSGIKFSYNHPSGYSHAVRTFVYQPVNVPTYCI*