AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1400190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1400190 MtrunA17_Chr8g0345501 65.169 89 31 0 24 112 408 496 7.13e-34 123
Msa1400190 MtrunA17_Chr2g0313001 72.500 40 11 0 7 46 1 40 2.43e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTATCTCGAGCCAAATT+TGG 0.138676 tig0022428:-10713 None:intergenic
TTACCTCTTTATCCCAAATT+TGG 0.184345 tig0022428:+10700 Msa1400190:three_prime_UTR
CCATCACCATCTGGAATTAT+TGG 0.275118 tig0022428:-9571 None:intergenic
AAGAAATTGCTCACATTATA+TGG 0.286562 tig0022428:+10108 Msa1400190:CDS
TGATTTCTTTGACTCATTCA+TGG 0.333269 tig0022428:+10187 Msa1400190:CDS
CCGTCATGGAAGCAATTATC+AGG 0.356730 tig0022428:+10152 Msa1400190:CDS
AACTATCTCGAGCCAAATTT+GGG 0.363047 tig0022428:-10712 None:intergenic
GTAGATTGAGTCAAAGTTGA+AGG 0.423072 tig0022428:+10412 Msa1400190:CDS
ACTTGTCATGATGCATGTTA+TGG 0.428964 tig0022428:-10510 None:intergenic
AATGTGTACATTTCAAGTTA+TGG 0.444448 tig0022428:+9489 Msa1400190:exon
CAAACTCACCCATCACCATC+TGG 0.453372 tig0022428:-9580 None:intergenic
CTCAGTTGATGCAAGAAGTT+CGG 0.458850 tig0022428:+10385 Msa1400190:CDS
TACAAGGCTTAAGCAACAAT+TGG 0.471347 tig0022428:+10442 Msa1400190:three_prime_UTR
TACATGGCAACCAGAGCATA+AGG 0.485592 tig0022428:+10317 Msa1400190:CDS
CTAACATGGCTAGCTGAAGA+TGG 0.497267 tig0022428:-9523 None:intergenic
TGCACATAGTAGTTCAGTTG+TGG 0.523431 tig0022428:+9446 Msa1400190:five_prime_UTR
CCAATAATTCCAGATGGTGA+TGG 0.531511 tig0022428:+9571 Msa1400190:CDS
GAGCCAAATTTGGGATAAAG+AGG 0.548288 tig0022428:-10703 None:intergenic
CAATAATTCCAGATGGTGAT+GGG 0.552654 tig0022428:+9572 Msa1400190:CDS
CAACGGTGTCTCTACTAACA+TGG 0.557176 tig0022428:-9537 None:intergenic
CCTGATAATTGCTTCCATGA+CGG 0.572248 tig0022428:-10152 None:intergenic
CATAGTAGTTCAGTTGTGGT+TGG 0.585520 tig0022428:+9450 Msa1400190:five_prime_UTR
TAGAAGAGGGAAAACCGTCA+TGG 0.601838 tig0022428:+10138 Msa1400190:CDS
TATATGGTCTCAATTAGAAG+AGG 0.602911 tig0022428:+10124 Msa1400190:CDS
TTTCTTTAACTACCAATGTG+TGG 0.607987 tig0022428:-10091 None:intergenic
AAAATACCAATAATTCCAGA+TGG 0.610541 tig0022428:+9565 Msa1400190:CDS
AGTTGAAGGAGGCAACTACA+AGG 0.610652 tig0022428:+10426 Msa1400190:exon
ATATGGTCTCAATTAGAAGA+GGG 0.627134 tig0022428:+10125 Msa1400190:CDS
CTTGAAATGTACACATTGCG+AGG 0.634227 tig0022428:-9483 None:intergenic
CAGGTTTGATCACCACACAT+TGG 0.634316 tig0022428:+10079 Msa1400190:intron
GATTGAGTCAAAGTTGAAGG+AGG 0.637853 tig0022428:+10415 Msa1400190:exon
ATGAGTCAAAGAAATCATCT+CGG 0.652827 tig0022428:-10181 None:intergenic
CATGTTAGTAGAGACACCGT+TGG 0.707519 tig0022428:+9538 Msa1400190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAATAATCATTATTATAT+GGG - tig0022428:9611-9630 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG + tig0022428:9623-9642 Msa1400190:intron 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG + tig0022428:9938-9957 Msa1400190:intron 15.0%
!! CCAAATAATCATTATTATAT+GGG - tig0022428:9611-9630 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG + tig0022428:9623-9642 Msa1400190:intron 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG + tig0022428:9938-9957 Msa1400190:intron 15.0%
!! CCCATATAATAATGATTATT+TGG + tig0022428:9608-9627 Msa1400190:intron 20.0%
!! GCCAAATAATCATTATTATA+TGG - tig0022428:9612-9631 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG + tig0022428:10301-10320 Msa1400190:intron 20.0%
!! CCCATATAATAATGATTATT+TGG + tig0022428:9608-9627 Msa1400190:intron 20.0%
!! GCCAAATAATCATTATTATA+TGG - tig0022428:9612-9631 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG + tig0022428:10301-10320 Msa1400190:intron 20.0%
!!! AATGTGTACATTTCAAGTTA+TGG + tig0022428:9489-9508 Msa1400190:exon 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG - tig0022428:9557-9576 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG + tig0022428:9565-9584 Msa1400190:CDS 25.0%
!! TTGTGGTATTATGATTTTAG+TGG + tig0022428:9706-9725 Msa1400190:intron 25.0%
!! TTTTCGTGTACATTATATTC+TGG + tig0022428:9755-9774 Msa1400190:intron 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG + tig0022428:9944-9963 Msa1400190:intron 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG + tig0022428:9982-10001 Msa1400190:intron 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG + tig0022428:10060-10079 Msa1400190:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG + tig0022428:10108-10127 Msa1400190:CDS 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG - tig0022428:10251-10270 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG + tig0022428:10346-10365 Msa1400190:CDS 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG + tig0022428:10347-10366 Msa1400190:CDS 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG + tig0022428:10348-10367 Msa1400190:CDS 25.0%
! AGCATATGCACAAAAAAAAA+TGG - tig0022428:10667-10686 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG + tig0022428:10672-10691 Msa1400190:three_prime_UTR 25.0%
!!! AATGTGTACATTTCAAGTTA+TGG + tig0022428:9489-9508 Msa1400190:exon 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG - tig0022428:9557-9576 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG + tig0022428:9565-9584 Msa1400190:CDS 25.0%
!! TTGTGGTATTATGATTTTAG+TGG + tig0022428:9706-9725 Msa1400190:intron 25.0%
!! TTTTCGTGTACATTATATTC+TGG + tig0022428:9755-9774 Msa1400190:intron 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG + tig0022428:9944-9963 Msa1400190:intron 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG + tig0022428:9982-10001 Msa1400190:intron 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG + tig0022428:10060-10079 Msa1400190:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG + tig0022428:10108-10127 Msa1400190:CDS 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG - tig0022428:10251-10270 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG + tig0022428:10346-10365 Msa1400190:CDS 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG + tig0022428:10347-10366 Msa1400190:CDS 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG + tig0022428:10348-10367 Msa1400190:CDS 25.0%
! AGCATATGCACAAAAAAAAA+TGG - tig0022428:10667-10686 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG + tig0022428:10672-10691 Msa1400190:three_prime_UTR 25.0%
! GATTCTGTTAACTTTCCTTT+TGG + tig0022428:9655-9674 Msa1400190:intron 30.0%
! TTTTCCTGAGAAAATATTGG+TGG + tig0022428:9985-10004 Msa1400190:intron 30.0%
CAAGTAAAGTCTTAACTCAA+AGG - tig0022428:10043-10062 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG - tig0022428:10094-10113 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG + tig0022428:10124-10143 Msa1400190:CDS 30.0%
ATATGGTCTCAATTAGAAGA+GGG + tig0022428:10125-10144 Msa1400190:CDS 30.0%
ATGAGTCAAAGAAATCATCT+CGG - tig0022428:10184-10203 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG + tig0022428:10187-10206 Msa1400190:CDS 30.0%
! GATTCTGTTAACTTTCCTTT+TGG + tig0022428:9655-9674 Msa1400190:intron 30.0%
! TTTTCCTGAGAAAATATTGG+TGG + tig0022428:9985-10004 Msa1400190:intron 30.0%
CAAGTAAAGTCTTAACTCAA+AGG - tig0022428:10043-10062 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG - tig0022428:10094-10113 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG + tig0022428:10124-10143 Msa1400190:CDS 30.0%
ATATGGTCTCAATTAGAAGA+GGG + tig0022428:10125-10144 Msa1400190:CDS 30.0%
ATGAGTCAAAGAAATCATCT+CGG - tig0022428:10184-10203 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG + tig0022428:10187-10206 Msa1400190:CDS 30.0%
CAATAATTCCAGATGGTGAT+GGG + tig0022428:9572-9591 Msa1400190:CDS 35.0%
! GCATTGTTACTTGATCCAAA+AGG - tig0022428:9673-9692 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG + tig0022428:9841-9860 Msa1400190:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG + tig0022428:10412-10431 Msa1400190:CDS 35.0%
TACAAGGCTTAAGCAACAAT+TGG + tig0022428:10442-10461 Msa1400190:three_prime_UTR 35.0%
ACTTGTCATGATGCATGTTA+TGG - tig0022428:10513-10532 None:intergenic 35.0%
CAATAATTCCAGATGGTGAT+GGG + tig0022428:9572-9591 Msa1400190:CDS 35.0%
! GCATTGTTACTTGATCCAAA+AGG - tig0022428:9673-9692 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG + tig0022428:9841-9860 Msa1400190:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG + tig0022428:10412-10431 Msa1400190:CDS 35.0%
TACAAGGCTTAAGCAACAAT+TGG + tig0022428:10442-10461 Msa1400190:three_prime_UTR 35.0%
ACTTGTCATGATGCATGTTA+TGG - tig0022428:10513-10532 None:intergenic 35.0%
TGCACATAGTAGTTCAGTTG+TGG + tig0022428:9446-9465 Msa1400190:five_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG + tig0022428:9450-9469 Msa1400190:five_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG - tig0022428:9486-9505 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG - tig0022428:9574-9593 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG + tig0022428:9571-9590 Msa1400190:CDS 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG + tig0022428:9951-9970 Msa1400190:intron 40.0%
! TGCACCACCAATATTTTCTC+AGG - tig0022428:9992-10011 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG + tig0022428:10007-10026 Msa1400190:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG - tig0022428:10155-10174 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG + tig0022428:10385-10404 Msa1400190:CDS 40.0%
GATTGAGTCAAAGTTGAAGG+AGG + tig0022428:10415-10434 Msa1400190:exon 40.0%
TGCACATAGTAGTTCAGTTG+TGG + tig0022428:9446-9465 Msa1400190:five_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG + tig0022428:9450-9469 Msa1400190:five_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG - tig0022428:9486-9505 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG - tig0022428:9574-9593 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG + tig0022428:9571-9590 Msa1400190:CDS 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG + tig0022428:9951-9970 Msa1400190:intron 40.0%
! TGCACCACCAATATTTTCTC+AGG - tig0022428:9992-10011 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG + tig0022428:10007-10026 Msa1400190:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG - tig0022428:10155-10174 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG + tig0022428:10385-10404 Msa1400190:CDS 40.0%
GATTGAGTCAAAGTTGAAGG+AGG + tig0022428:10415-10434 Msa1400190:exon 40.0%
! CTAACATGGCTAGCTGAAGA+TGG - tig0022428:9526-9545 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG - tig0022428:9540-9559 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG + tig0022428:9538-9557 Msa1400190:CDS 45.0%
ATGCAAGACTGTTGTGCTTG+TGG + tig0022428:9689-9708 Msa1400190:intron 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG + tig0022428:9955-9974 Msa1400190:intron 45.0%
CAGGTTTGATCACCACACAT+TGG + tig0022428:10079-10098 Msa1400190:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG + tig0022428:10138-10157 Msa1400190:CDS 45.0%
CCGTCATGGAAGCAATTATC+AGG + tig0022428:10152-10171 Msa1400190:CDS 45.0%
TACATGGCAACCAGAGCATA+AGG + tig0022428:10317-10336 Msa1400190:CDS 45.0%
! CGCACTTTTACCTTATGCTC+TGG - tig0022428:10330-10349 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG + tig0022428:10426-10445 Msa1400190:exon 45.0%
! CTAACATGGCTAGCTGAAGA+TGG - tig0022428:9526-9545 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG - tig0022428:9540-9559 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG + tig0022428:9538-9557 Msa1400190:CDS 45.0%
ATGCAAGACTGTTGTGCTTG+TGG + tig0022428:9689-9708 Msa1400190:intron 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG + tig0022428:9955-9974 Msa1400190:intron 45.0%
CAGGTTTGATCACCACACAT+TGG + tig0022428:10079-10098 Msa1400190:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG + tig0022428:10138-10157 Msa1400190:CDS 45.0%
CCGTCATGGAAGCAATTATC+AGG + tig0022428:10152-10171 Msa1400190:CDS 45.0%
TACATGGCAACCAGAGCATA+AGG + tig0022428:10317-10336 Msa1400190:CDS 45.0%
! CGCACTTTTACCTTATGCTC+TGG - tig0022428:10330-10349 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG + tig0022428:10426-10445 Msa1400190:exon 45.0%
CAAACTCACCCATCACCATC+TGG - tig0022428:9583-9602 None:intergenic 50.0%
CAAACTCACCCATCACCATC+TGG - tig0022428:9583-9602 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
tig0022428 gene 9384 10720 9384 ID=Msa1400190;Name=Msa1400190
tig0022428 mRNA 9384 10720 9384 ID=Msa1400190-mRNA-1;Parent=Msa1400190;Name=Msa1400190-mRNA-1;_AED=0.28;_eAED=0.28;_QI=106|1|1|1|1|1|3|289|117
tig0022428 exon 9384 9593 9384 ID=Msa1400190-mRNA-1:exon:17666;Parent=Msa1400190-mRNA-1
tig0022428 exon 10082 10208 10082 ID=Msa1400190-mRNA-1:exon:17667;Parent=Msa1400190-mRNA-1
tig0022428 exon 10309 10720 10309 ID=Msa1400190-mRNA-1:exon:17668;Parent=Msa1400190-mRNA-1
tig0022428 five_prime_UTR 9384 9489 9384 ID=Msa1400190-mRNA-1:five_prime_utr;Parent=Msa1400190-mRNA-1
tig0022428 CDS 9490 9593 9490 ID=Msa1400190-mRNA-1:cds;Parent=Msa1400190-mRNA-1
tig0022428 CDS 10082 10208 10082 ID=Msa1400190-mRNA-1:cds;Parent=Msa1400190-mRNA-1
tig0022428 CDS 10309 10431 10309 ID=Msa1400190-mRNA-1:cds;Parent=Msa1400190-mRNA-1
tig0022428 three_prime_UTR 10432 10720 10432 ID=Msa1400190-mRNA-1:three_prime_utr;Parent=Msa1400190-mRNA-1
Gene Sequence

>Msa1400190

ATGTGTACATTTCAAGTTATGGTCAATAGAGCTCCATCTTCAGCTAGCCATGTTAGTAGAGACACCGTTGGTACTAAAATACCAATAATTCCAGATGGTGATGGGTTTGATCACCACACATTGGTAGTTAAAGAAATTGCTCACATTATATGGTCTCAATTAGAAGAGGGAAAACCGTCATGGAAGCAATTATCAGGAAATCACCGAGATGATTTCTTTGACTCATTCATGAAAAAGTTTACATGGCAACCAGAGCATAAGGTAAAAGTGCGAAAAAACTTTGAAATAAGGGGTGCAGCTAAGATGTCTCAGTTGATGCAAGAAGTTCGGAAAAGTAGATTGAGTCAAAGTTGA

Protein sequence

>Msa1400190

MCTFQVMVNRAPSSASHVSRDTVGTKIPIIPDGDGFDHHTLVVKEIAHIIWSQLEEGKPSWKQLSGNHRDDFFDSFMKKFTWQPEHKVKVRKNFEIRGAAKMSQLMQEVRKSRLSQS*