AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0827780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAATTGCTCACATTATA+CGG 0.326347 5_3:+69262200 Msa0827780:five_prime_UTR
TGATTTCTTTGACTCATTCA+TGG 0.333269 5_3:+69262279 Msa0827780:exon
CCGTCATGGAAGCAATTATC+AGG 0.356730 5_3:+69262244 Msa0827780:five_prime_UTR
GTAGATTGAGTCAAAGTTGA+AGG 0.423072 5_3:+69262506 Msa0827780:intron
ACTTGTCATGATGCATGTTA+TGG 0.428964 5_3:-69262604 None:intergenic
TACAAGGCTTAAGCAACAAT+TGG 0.471347 5_3:+69262536 Msa0827780:CDS
TACATGGCAACCAGAGCATA+AGG 0.485592 5_3:+69262410 Msa0827780:CDS
GTGCAAGTAGTTAGTTGTGT+TGG 0.547526 5_3:+69262099 None:intergenic
CAAGTAAAGTCTTAACTCAA+AGG 0.556719 5_3:-69262132 None:intergenic
CCTGATAATTGCTTCCATGA+CGG 0.572248 5_3:-69262244 None:intergenic
TAGAAGAGGGAAAACCGTCA+TGG 0.601838 5_3:+69262230 Msa0827780:five_prime_UTR
TTTCTTTAACTACCAATGTG+TGG 0.607987 5_3:-69262183 None:intergenic
AGTTGAAGGAGGCAACTACA+AGG 0.610652 5_3:+69262520 Msa0827780:CDS
TATACGGTCTCAATTAGAAG+AGG 0.616182 5_3:+69262216 Msa0827780:five_prime_UTR
CAGGTTTGATCACCACACAT+TGG 0.634316 5_3:+69262171 Msa0827780:five_prime_UTR
GATTGAGTCAAAGTTGAAGG+AGG 0.637853 5_3:+69262509 Msa0827780:intron
ATACGGTCTCAATTAGAAGA+GGG 0.649151 5_3:+69262217 Msa0827780:five_prime_UTR
ATGAGTCAAAGAAATCATCT+CGG 0.652827 5_3:-69262273 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTACAGAAAAAGTTTACA+TGG + chr5_3:69262394-69262413 Msa0827780:intron 20.0%
!!! CGAAAAAAACTTTGAAATAA+GGG + chr5_3:69262441-69262460 Msa0827780:intron 20.0%
!!! GAAAAAAACTTTGAAATAAG+GGG + chr5_3:69262442-69262461 Msa0827780:intron 20.0%
!!! TTGTTTGAAACACATTTTTC+AGG + chr5_3:69262152-69262171 Msa0827780:five_prime_UTR 25.0%
! AAGAAATTGCTCACATTATA+CGG + chr5_3:69262200-69262219 Msa0827780:five_prime_UTR 25.0%
!!! TACTTAAATCTGTTCTAAAG+AGG - chr5_3:69262343-69262362 None:intergenic 25.0%
!!! GCGAAAAAAACTTTGAAATA+AGG + chr5_3:69262440-69262459 Msa0827780:intron 25.0%
CAAGTAAAGTCTTAACTCAA+AGG - chr5_3:69262135-69262154 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG - chr5_3:69262186-69262205 None:intergenic 30.0%
ATGAGTCAAAGAAATCATCT+CGG - chr5_3:69262276-69262295 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG + chr5_3:69262279-69262298 Msa0827780:exon 30.0%
TATACGGTCTCAATTAGAAG+AGG + chr5_3:69262216-69262235 Msa0827780:five_prime_UTR 35.0%
ATACGGTCTCAATTAGAAGA+GGG + chr5_3:69262217-69262236 Msa0827780:five_prime_UTR 35.0%
GTAGATTGAGTCAAAGTTGA+AGG + chr5_3:69262506-69262525 Msa0827780:intron 35.0%
TACAAGGCTTAAGCAACAAT+TGG + chr5_3:69262536-69262555 Msa0827780:CDS 35.0%
ACTTGTCATGATGCATGTTA+TGG - chr5_3:69262607-69262626 None:intergenic 35.0%
CCTGATAATTGCTTCCATGA+CGG - chr5_3:69262247-69262266 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG + chr5_3:69262479-69262498 Msa0827780:intron 40.0%
GATTGAGTCAAAGTTGAAGG+AGG + chr5_3:69262509-69262528 Msa0827780:intron 40.0%
CAGGTTTGATCACCACACAT+TGG + chr5_3:69262171-69262190 Msa0827780:five_prime_UTR 45.0%
TAGAAGAGGGAAAACCGTCA+TGG + chr5_3:69262230-69262249 Msa0827780:five_prime_UTR 45.0%
CCGTCATGGAAGCAATTATC+AGG + chr5_3:69262244-69262263 Msa0827780:five_prime_UTR 45.0%
TACATGGCAACCAGAGCATA+AGG + chr5_3:69262410-69262429 Msa0827780:CDS 45.0%
! CGCACTTTTACCTTATGCTC+TGG - chr5_3:69262423-69262442 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG + chr5_3:69262520-69262539 Msa0827780:CDS 45.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 69262100 69262723 69262100 ID=Msa0827780;Name=Msa0827780
chr5_3 mRNA 69262100 69262723 69262100 ID=Msa0827780-mRNA-1;Parent=Msa0827780;Name=Msa0827780-mRNA-1;_AED=0.19;_eAED=0.19;_QI=198|0.33|0.25|1|0|0|4|0|62
chr5_3 exon 69262100 69262300 69262100 ID=Msa0827780-mRNA-1:exon:21782;Parent=Msa0827780-mRNA-1
chr5_3 exon 69262402 69262431 69262402 ID=Msa0827780-mRNA-1:exon:21783;Parent=Msa0827780-mRNA-1
chr5_3 exon 69262510 69262629 69262510 ID=Msa0827780-mRNA-1:exon:21784;Parent=Msa0827780-mRNA-1
chr5_3 exon 69262691 69262723 69262691 ID=Msa0827780-mRNA-1:exon:21785;Parent=Msa0827780-mRNA-1
chr5_3 five_prime_UTR 69262100 69262297 69262100 ID=Msa0827780-mRNA-1:five_prime_utr;Parent=Msa0827780-mRNA-1
chr5_3 CDS 69262298 69262300 69262298 ID=Msa0827780-mRNA-1:cds;Parent=Msa0827780-mRNA-1
chr5_3 CDS 69262402 69262431 69262402 ID=Msa0827780-mRNA-1:cds;Parent=Msa0827780-mRNA-1
chr5_3 CDS 69262510 69262629 69262510 ID=Msa0827780-mRNA-1:cds;Parent=Msa0827780-mRNA-1
chr5_3 CDS 69262691 69262723 69262691 ID=Msa0827780-mRNA-1:cds;Parent=Msa0827780-mRNA-1
Gene Sequence

>Msa0827780

ATGAAAAAGTTTACATGGCAACCAGAGCATAAGATTGAGTCAAAGTTGAAGGAGGCAACTACAAGGCTTAAGCAACAATTGGCAGAAGAACAAGCTGCGCGACTAAGAGCAGAAGACATTGCAAATTCCATAACATGCATCATGACAAGTTTTCTTCATAGTGATGAAGACTTTGTATTTTATGAC

Protein sequence

>Msa0827780

MKKFTWQPEHKIESKLKEATTRLKQQLAEEQAARLRAEDIANSITCIMTSFLHSDEDFVFYD