Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438740 | XP_003597762.1 | 88.942 | 208 | 16 | 3 | 1 | 206 | 1 | 203 | 3.48e-123 | 357 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438740 | G7II32 | 88.942 | 208 | 16 | 3 | 1 | 206 | 1 | 203 | 1.66e-123 | 357 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016670 | Msa1438740 | 0.850330 | 1.821024e-60 | -8.615850e-47 |
| Msa0017510 | Msa1438740 | 0.847757 | 9.450471e-60 | -8.615850e-47 |
| Msa0032840 | Msa1438740 | -0.808473 | 3.044041e-50 | -8.615850e-47 |
| Msa0034270 | Msa1438740 | 0.807245 | 5.554972e-50 | -8.615850e-47 |
| Msa0036430 | Msa1438740 | 0.813140 | 2.975733e-51 | -8.615850e-47 |
| Msa0072370 | Msa1438740 | 0.816204 | 6.236771e-52 | -8.615850e-47 |
| Msa0081360 | Msa1438740 | 0.807769 | 4.299144e-50 | -8.615850e-47 |
| Msa0081480 | Msa1438740 | 0.800040 | 1.736337e-48 | -8.615850e-47 |
| Msa0090590 | Msa1438740 | 0.810889 | 9.209568e-51 | -8.615850e-47 |
| Msa0094120 | Msa1438740 | 0.802864 | 4.581536e-49 | -8.615850e-47 |
| Msa0103000 | Msa1438740 | 0.811216 | 7.821723e-51 | -8.615850e-47 |
| Msa0110360 | Msa1438740 | 0.807853 | 4.125519e-50 | -8.615850e-47 |
| Msa0110970 | Msa1438740 | 0.863283 | 2.779679e-64 | -8.615850e-47 |
| Msa0114540 | Msa1438740 | -0.814825 | 1.264985e-51 | -8.615850e-47 |
| Msa0115160 | Msa1438740 | 0.810268 | 1.254470e-50 | -8.615850e-47 |
| Msa1351760 | Msa1438740 | 0.828855 | 7.127407e-55 | -8.615850e-47 |
| Msa1364470 | Msa1438740 | -0.806204 | 9.213225e-50 | -8.615850e-47 |
| Msa1374550 | Msa1438740 | 0.802698 | 4.957516e-49 | -8.615850e-47 |
| Msa1377710 | Msa1438740 | 0.816274 | 6.016673e-52 | -8.615850e-47 |
| Msa1380280 | Msa1438740 | 0.811248 | 7.697155e-51 | -8.615850e-47 |
| Msa1429360 | Msa1438740 | -0.805936 | 1.049374e-49 | -8.615850e-47 |
| Msa1438740 | Msa1461300 | 0.802919 | 4.462938e-49 | -8.615850e-47 |
| Msa0624510 | Msa1438740 | 0.928081 | 4.779985e-92 | -8.615850e-47 |
| Msa0647130 | Msa1438740 | 0.826645 | 2.421938e-54 | -8.615850e-47 |
| Msa0650090 | Msa1438740 | 0.826147 | 3.181181e-54 | -8.615850e-47 |
| Msa0684500 | Msa1438740 | 0.832761 | 7.860917e-56 | -8.615850e-47 |
| Msa0692080 | Msa1438740 | 0.823033 | 1.721859e-53 | -8.615850e-47 |
| Msa0703230 | Msa1438740 | 0.812702 | 3.710970e-51 | -8.615850e-47 |
| Msa0754520 | Msa1438740 | 0.804868 | 1.756769e-49 | -8.615850e-47 |
| Msa0760380 | Msa1438740 | 0.822645 | 2.120646e-53 | -8.615850e-47 |
| Msa0806300 | Msa1438740 | 0.805537 | 1.272699e-49 | -8.615850e-47 |
| Msa0813740 | Msa1438740 | 0.802087 | 6.626898e-49 | -8.615850e-47 |
| Msa0833220 | Msa1438740 | 0.809783 | 1.595058e-50 | -8.615850e-47 |
| Msa0124340 | Msa1438740 | 0.816861 | 4.446319e-52 | -8.615850e-47 |
| Msa0130570 | Msa1438740 | 0.812832 | 3.476869e-51 | -8.615850e-47 |
| Msa0138820 | Msa1438740 | -0.819225 | 1.298488e-52 | -8.615850e-47 |
| Msa0147560 | Msa1438740 | 0.800575 | 1.351395e-48 | -8.615850e-47 |
| Msa0157290 | Msa1438740 | 0.848075 | 7.719222e-60 | -8.615850e-47 |
| Msa0182620 | Msa1438740 | -0.807806 | 4.221836e-50 | -8.615850e-47 |
| Msa0183830 | Msa1438740 | 0.801955 | 7.052240e-49 | -8.615850e-47 |
| Msa0186890 | Msa1438740 | 0.811768 | 5.934465e-51 | -8.615850e-47 |
| Msa0225740 | Msa1438740 | 0.807177 | 5.741655e-50 | -8.615850e-47 |
| Msa0230550 | Msa1438740 | -0.809097 | 2.238791e-50 | -8.615850e-47 |
| Msa1252190 | Msa1438740 | 0.800288 | 1.546213e-48 | -8.615850e-47 |
| Msa1269580 | Msa1438740 | -0.802301 | 5.987604e-49 | -8.615850e-47 |
| Msa1323670 | Msa1438740 | 0.804776 | 1.836669e-49 | -8.615850e-47 |
| Msa0375090 | Msa1438740 | 0.801574 | 8.442385e-49 | -8.615850e-47 |
| Msa0422850 | Msa1438740 | 0.808652 | 2.788406e-50 | -8.615850e-47 |
| Msa0422930 | Msa1438740 | 0.808851 | 2.527217e-50 | -8.615850e-47 |
| Msa0436280 | Msa1438740 | -0.806662 | 7.378406e-50 | -8.615850e-47 |
| Msa0461720 | Msa1438740 | 0.815771 | 7.791949e-52 | -8.615850e-47 |
| Msa1003030 | Msa1438740 | 0.800106 | 1.683478e-48 | -8.615850e-47 |
| Msa1004550 | Msa1438740 | 0.800655 | 1.301763e-48 | -8.615850e-47 |
| Msa1004600 | Msa1438740 | 0.816914 | 4.325077e-52 | -8.615850e-47 |
| Msa1012810 | Msa1438740 | 0.809186 | 2.142779e-50 | -8.615850e-47 |
| Msa1019020 | Msa1438740 | 0.804338 | 2.266396e-49 | -8.615850e-47 |
| Msa1031930 | Msa1438740 | 0.807274 | 5.475976e-50 | -8.615850e-47 |
| Msa1037920 | Msa1438740 | 0.802287 | 6.026645e-49 | -8.615850e-47 |
| Msa1048920 | Msa1438740 | 0.803127 | 4.043468e-49 | -8.615850e-47 |
| Msa1080870 | Msa1438740 | 0.805941 | 1.046527e-49 | -8.615850e-47 |
| Msa1097560 | Msa1438740 | 0.808776 | 2.623361e-50 | -8.615850e-47 |
| Msa1097640 | Msa1438740 | 0.822870 | 1.879625e-53 | -8.615850e-47 |
| Msa0264350 | Msa1438740 | 0.800046 | 1.732157e-48 | -8.615850e-47 |
| Msa0292400 | Msa1438740 | 0.801212 | 1.001724e-48 | -8.615850e-47 |
| Msa0306300 | Msa1438740 | 0.832433 | 9.477707e-56 | -8.615850e-47 |
| Msa0316850 | Msa1438740 | 0.807578 | 4.721427e-50 | -8.615850e-47 |
| Msa1127890 | Msa1438740 | 0.813440 | 2.557497e-51 | -8.615850e-47 |
| Msa1127950 | Msa1438740 | 0.812640 | 3.829177e-51 | -8.615850e-47 |
| Msa1139810 | Msa1438740 | 0.834213 | 3.412403e-56 | -8.615850e-47 |
| Msa1147570 | Msa1438740 | 0.801259 | 9.795094e-49 | -8.615850e-47 |
| Msa1172450 | Msa1438740 | 0.807591 | 4.690349e-50 | -8.615850e-47 |
| Msa1187840 | Msa1438740 | 0.805236 | 1.471548e-49 | -8.615850e-47 |
| Msa1218280 | Msa1438740 | -0.814079 | 1.849039e-51 | -8.615850e-47 |
| Msa0503560 | Msa1438740 | 0.807863 | 4.106114e-50 | -8.615850e-47 |
| Msa0503580 | Msa1438740 | 0.814671 | 1.368129e-51 | -8.615850e-47 |
| Msa0527550 | Msa1438740 | 0.809849 | 1.543990e-50 | -8.615850e-47 |
| Msa0541860 | Msa1438740 | 0.810699 | 1.012449e-50 | -8.615850e-47 |
| Msa0555910 | Msa1438740 | 0.803186 | 3.931344e-49 | -8.615850e-47 |
| Msa0574890 | Msa1438740 | 0.811388 | 7.179182e-51 | -8.615850e-47 |
| Msa0844240 | Msa1438740 | 0.804380 | 2.221248e-49 | -8.615850e-47 |
| Msa0872310 | Msa1438740 | 0.814020 | 1.905161e-51 | -8.615850e-47 |
| Msa0880140 | Msa1438740 | 0.847628 | 1.025362e-59 | -8.615850e-47 |
| Msa0893480 | Msa1438740 | -0.807746 | 4.348353e-50 | -8.615850e-47 |
| Msa0930780 | Msa1438740 | 0.814166 | 1.769705e-51 | -8.615850e-47 |
| Msa0936340 | Msa1438740 | 0.804874 | 1.751586e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438740 | MtrunA17_Chr2g0331751 | 88.942 | 208 | 16 | 3 | 1 | 206 | 1 | 203 | 3.20e-127 | 357 |
| Msa1438740 | MtrunA17_Chr4g0004611 | 55.024 | 209 | 79 | 7 | 3 | 206 | 4 | 202 | 5.15e-63 | 194 |
| Msa1438740 | MtrunA17_Chr8g0390541 | 33.175 | 211 | 126 | 6 | 1 | 205 | 1 | 202 | 8.89e-23 | 91.3 |
| Msa1438740 | MtrunA17_Chr6g0485651 | 37.234 | 188 | 92 | 5 | 1 | 182 | 1 | 168 | 1.78e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438740 | AT3G16070.1 | 32.759 | 174 | 89 | 5 | 9 | 181 | 4 | 150 | 2.84e-20 | 83.6 |
| Msa1438740 | AT1G15260.1 | 35.784 | 204 | 97 | 9 | 5 | 206 | 7 | 178 | 1.47e-19 | 82.4 |
Find 21 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTCAAATGTTTCATCTT+TGG | 0.211526 | tig0028621:-8403 | Msa1438740:CDS |
| CTTTCCTTCAATCCATTCTT+AGG | 0.265660 | tig0028621:-8355 | Msa1438740:CDS |
| TCCACAATATCTAAATAAAT+AGG | 0.347318 | tig0028621:+7965 | None:intergenic |
| GAATTAGAGATGAATGATTC+AGG | 0.387057 | tig0028621:-8049 | Msa1438740:CDS |
| GAATGAAGGTGTTAATGAAT+TGG | 0.390905 | tig0028621:-8086 | Msa1438740:CDS |
| TCTAAGTCAAAGGGAAGATT+TGG | 0.402476 | tig0028621:-7878 | Msa1438740:CDS |
| AGGGAAGATTTGGATCAATC+AGG | 0.432753 | tig0028621:-7868 | Msa1438740:CDS |
| TTCAAATGTTTCATCTTTGG+AGG | 0.433543 | tig0028621:-8400 | Msa1438740:CDS |
| TCCTATTTATTTAGATATTG+TGG | 0.439985 | tig0028621:-7966 | Msa1438740:CDS |
| CTGATCTCAATAGAATAAAC+AGG | 0.442469 | tig0028621:-8261 | Msa1438740:CDS |
| TGAATTGGCATTGAAGATGA+AGG | 0.456133 | tig0028621:-8071 | Msa1438740:CDS |
| GGATTGAAGGAAAGAGATCT+AGG | 0.460419 | tig0028621:+8364 | None:intergenic |
| TTGCTACTTCTACCTAAGAA+TGG | 0.464176 | tig0028621:+8343 | None:intergenic |
| TCTACCTAAGAATGGATTGA+AGG | 0.485195 | tig0028621:+8351 | None:intergenic |
| AAAGGGAATAAGAAGAATGA+AGG | 0.496505 | tig0028621:-8100 | Msa1438740:CDS |
| ATCAAACGCTCTAAGTCAAA+GGG | 0.509484 | tig0028621:-7887 | Msa1438740:CDS |
| CATCAAACGCTCTAAGTCAA+AGG | 0.517132 | tig0028621:-7888 | Msa1438740:CDS |
| CTCAATAGAATAAACAGGAA+TGG | 0.562291 | tig0028621:-8256 | Msa1438740:CDS |
| TCAAATGTTTCATCTTTGGA+GGG | 0.578974 | tig0028621:-8399 | Msa1438740:CDS |
| AGAGCGTTTGATGCTGACAG+AGG | 0.645792 | tig0028621:+7898 | None:intergenic |
| CGTGTCGAGAGAACAAACAA+TGG | 0.658228 | tig0028621:-8218 | Msa1438740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTTTTAAGTAAAAATT+TGG | + | tig0028621:7893-7912 | None:intergenic | 10.0% |
| !!! | TAAAAATTTGGTTTTGCAAA+TGG | + | tig0028621:7881-7900 | None:intergenic | 20.0% |
| !! | TCCTATTTATTTAGATATTG+TGG | - | tig0028621:8356-8375 | Msa1438740:CDS | 20.0% |
| !! | TCCACAATATCTAAATAAAT+AGG | + | tig0028621:8360-8379 | None:intergenic | 20.0% |
| ! | AACTTCAAATGTTTCATCTT+TGG | - | tig0028621:7919-7938 | Msa1438740:CDS | 25.0% |
| !!! | TTTTCAGTAAGGTTTTTTTG+TGG | + | tig0028621:8180-8199 | None:intergenic | 25.0% |
| ! | CAAAAAAACCTTACTGAAAA+TGG | - | tig0028621:8180-8199 | Msa1438740:CDS | 25.0% |
| ! | AGAAAGAGAAAAAAGACAAA+GGG | - | tig0028621:8205-8224 | Msa1438740:CDS | 25.0% |
| TTCAAATGTTTCATCTTTGG+AGG | - | tig0028621:7922-7941 | Msa1438740:CDS | 30.0% | |
| TCAAATGTTTCATCTTTGGA+GGG | - | tig0028621:7923-7942 | Msa1438740:CDS | 30.0% | |
| ! | AAAGAGATCTAGGTAGTTTT+TGG | + | tig0028621:7951-7970 | None:intergenic | 30.0% |
| CTGATCTCAATAGAATAAAC+AGG | - | tig0028621:8061-8080 | Msa1438740:CDS | 30.0% | |
| CTCAATAGAATAAACAGGAA+TGG | - | tig0028621:8066-8085 | Msa1438740:CDS | 30.0% | |
| GAGAAAGAGAAAAAAGACAA+AGG | - | tig0028621:8204-8223 | Msa1438740:CDS | 30.0% | |
| AAAGGGAATAAGAAGAATGA+AGG | - | tig0028621:8222-8241 | Msa1438740:CDS | 30.0% | |
| !! | GAATGAAGGTGTTAATGAAT+TGG | - | tig0028621:8236-8255 | Msa1438740:CDS | 30.0% |
| GAATTAGAGATGAATGATTC+AGG | - | tig0028621:8273-8292 | Msa1438740:CDS | 30.0% | |
| CTTTCCTTCAATCCATTCTT+AGG | - | tig0028621:7967-7986 | Msa1438740:CDS | 35.0% | |
| !! | TCTACCTAAGAATGGATTGA+AGG | + | tig0028621:7974-7993 | None:intergenic | 35.0% |
| ! | TTGCTACTTCTACCTAAGAA+TGG | + | tig0028621:7982-8001 | None:intergenic | 35.0% |
| ! | CTTTCTCTCCATTTTCAGTA+AGG | + | tig0028621:8191-8210 | None:intergenic | 35.0% |
| ! | TGAATTGGCATTGAAGATGA+AGG | - | tig0028621:8251-8270 | Msa1438740:CDS | 35.0% |
| ! | ATCAAACGCTCTAAGTCAAA+GGG | - | tig0028621:8435-8454 | Msa1438740:CDS | 35.0% |
| TCTAAGTCAAAGGGAAGATT+TGG | - | tig0028621:8444-8463 | Msa1438740:CDS | 35.0% | |
| GGATTGAAGGAAAGAGATCT+AGG | + | tig0028621:7961-7980 | None:intergenic | 40.0% | |
| ! | CATCAAACGCTCTAAGTCAA+AGG | - | tig0028621:8434-8453 | Msa1438740:CDS | 40.0% |
| AGGGAAGATTTGGATCAATC+AGG | - | tig0028621:8454-8473 | Msa1438740:CDS | 40.0% | |
| CGTGTCGAGAGAACAAACAA+TGG | - | tig0028621:8104-8123 | Msa1438740:CDS | 45.0% | |
| ! | AGAGCGTTTGATGCTGACAG+AGG | + | tig0028621:8427-8446 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0028621 | gene | 7862 | 8482 | 7862 | ID=Msa1438740;Name=Msa1438740 |
| tig0028621 | mRNA | 7862 | 8482 | 7862 | ID=Msa1438740-mRNA-1;Parent=Msa1438740;Name=Msa1438740-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|206 |
| tig0028621 | exon | 7862 | 8482 | 7862 | ID=Msa1438740-mRNA-1:exon:11402;Parent=Msa1438740-mRNA-1 |
| tig0028621 | CDS | 7862 | 8482 | 7862 | ID=Msa1438740-mRNA-1:cds;Parent=Msa1438740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
