Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0624510 | XP_003597762.1 | 93.750 | 208 | 6 | 3 | 1 | 206 | 1 | 203 | 1.52e-133 | 383 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016670 | Msa0624510 | 0.848602 | 5.519898e-60 | -8.615850e-47 |
| Msa0017510 | Msa0624510 | 0.817179 | 3.771626e-52 | -8.615850e-47 |
| Msa0032840 | Msa0624510 | -0.803583 | 3.253309e-49 | -8.615850e-47 |
| Msa0034270 | Msa0624510 | 0.807288 | 5.439043e-50 | -8.615850e-47 |
| Msa0036430 | Msa0624510 | 0.809598 | 1.748006e-50 | -8.615850e-47 |
| Msa0072370 | Msa0624510 | 0.832231 | 1.063535e-55 | -8.615850e-47 |
| Msa0072810 | Msa0624510 | 0.817206 | 3.718566e-52 | -8.615850e-47 |
| Msa0081360 | Msa0624510 | 0.810902 | 9.150651e-51 | -8.615850e-47 |
| Msa0081480 | Msa0624510 | 0.803238 | 3.835152e-49 | -8.615850e-47 |
| Msa0090590 | Msa0624510 | 0.808571 | 2.900601e-50 | -8.615850e-47 |
| Msa0094120 | Msa0624510 | 0.802484 | 5.490161e-49 | -8.615850e-47 |
| Msa0103000 | Msa0624510 | 0.830160 | 3.434769e-55 | -8.615850e-47 |
| Msa0110970 | Msa0624510 | 0.860576 | 1.876279e-63 | -8.615850e-47 |
| Msa0114540 | Msa0624510 | -0.808481 | 3.031950e-50 | -8.615850e-47 |
| Msa0115160 | Msa0624510 | 0.820365 | 7.127462e-53 | -8.615850e-47 |
| Msa0118570 | Msa0624510 | 0.808066 | 3.717125e-50 | -8.615850e-47 |
| Msa0609450 | Msa0624510 | 0.814385 | 1.582386e-51 | -8.615850e-47 |
| Msa0624510 | Msa0650090 | 0.818016 | 2.442262e-52 | -8.615850e-47 |
| Msa0624510 | Msa0684500 | 0.831816 | 1.346620e-55 | -8.615850e-47 |
| Msa0624510 | Msa0692080 | 0.822634 | 2.132351e-53 | -8.615850e-47 |
| Msa0624510 | Msa0702340 | 0.811511 | 6.748981e-51 | -8.615850e-47 |
| Msa0624510 | Msa0702590 | 0.813545 | 2.424391e-51 | -8.615850e-47 |
| Msa0624510 | Msa0702660 | 0.817026 | 4.082541e-52 | -8.615850e-47 |
| Msa0624510 | Msa0703230 | 0.819248 | 1.282905e-52 | -8.615850e-47 |
| Msa0624510 | Msa0742740 | 0.802427 | 5.639099e-49 | -8.615850e-47 |
| Msa0624510 | Msa0743200 | 0.801381 | 9.248978e-49 | -8.615850e-47 |
| Msa0624510 | Msa0749760 | 0.800672 | 1.291010e-48 | -8.615850e-47 |
| Msa0624510 | Msa0754520 | 0.803783 | 2.955679e-49 | -8.615850e-47 |
| Msa0624510 | Msa0760380 | 0.820980 | 5.149244e-53 | -8.615850e-47 |
| Msa0624510 | Msa0779870 | 0.810843 | 9.420182e-51 | -8.615850e-47 |
| Msa0624510 | Msa0780740 | 0.805417 | 1.348554e-49 | -8.615850e-47 |
| Msa0624510 | Msa0823820 | 0.817238 | 3.657719e-52 | -8.615850e-47 |
| Msa0624510 | Msa0833220 | 0.806487 | 8.033706e-50 | -8.615850e-47 |
| Msa0624510 | Msa0844240 | 0.800423 | 1.451071e-48 | -8.615850e-47 |
| Msa0624510 | Msa0861510 | 0.810848 | 9.396467e-51 | -8.615850e-47 |
| Msa0624510 | Msa0861530 | 0.801731 | 7.839412e-49 | -8.615850e-47 |
| Msa0624510 | Msa0861600 | 0.803373 | 3.595681e-49 | -8.615850e-47 |
| Msa0624510 | Msa0863010 | 0.815032 | 1.138094e-51 | -8.615850e-47 |
| Msa0624510 | Msa0863370 | 0.812251 | 4.657783e-51 | -8.615850e-47 |
| Msa0624510 | Msa0872310 | 0.801087 | 1.062489e-48 | -8.615850e-47 |
| Msa0624510 | Msa0880140 | 0.817208 | 3.715552e-52 | -8.615850e-47 |
| Msa0624510 | Msa0893480 | -0.802311 | 5.959712e-49 | -8.615850e-47 |
| Msa0624510 | Msa0930780 | 0.832456 | 9.357532e-56 | -8.615850e-47 |
| Msa0624510 | Msa0936340 | 0.804255 | 2.358837e-49 | -8.615850e-47 |
| Msa0624510 | Msa0974060 | 0.808648 | 2.793021e-50 | -8.615850e-47 |
| Msa0624510 | Msa1003030 | 0.802632 | 5.115371e-49 | -8.615850e-47 |
| Msa0624510 | Msa1004550 | 0.800119 | 1.673618e-48 | -8.615850e-47 |
| Msa0624510 | Msa1004600 | 0.806105 | 9.666040e-50 | -8.615850e-47 |
| Msa0624510 | Msa1012810 | 0.801246 | 9.857570e-49 | -8.615850e-47 |
| Msa0624510 | Msa1031930 | 0.827240 | 1.744672e-54 | -8.615850e-47 |
| Msa0624510 | Msa1048920 | 0.801109 | 1.051448e-48 | -8.615850e-47 |
| Msa0624510 | Msa1063950 | 0.820161 | 7.938001e-53 | -8.615850e-47 |
| Msa0624510 | Msa1097640 | 0.823746 | 1.173428e-53 | -8.615850e-47 |
| Msa0624510 | Msa1108160 | 0.802739 | 4.863059e-49 | -8.615850e-47 |
| Msa0624510 | Msa1127890 | 0.814417 | 1.557444e-51 | -8.615850e-47 |
| Msa0624510 | Msa1127950 | 0.813474 | 2.513077e-51 | -8.615850e-47 |
| Msa0624510 | Msa1139810 | 0.829866 | 4.049586e-55 | -8.615850e-47 |
| Msa0624510 | Msa1165870 | 0.811188 | 7.931560e-51 | -8.615850e-47 |
| Msa0624510 | Msa1166400 | 0.800931 | 1.143526e-48 | -8.615850e-47 |
| Msa0624510 | Msa1172450 | 0.804053 | 2.598203e-49 | -8.615850e-47 |
| Msa0624510 | Msa1187840 | 0.801373 | 9.283217e-49 | -8.615850e-47 |
| Msa0624510 | Msa1218280 | -0.810523 | 1.104968e-50 | -8.615850e-47 |
| Msa0624510 | Msa1218600 | 0.818106 | 2.329887e-52 | -8.615850e-47 |
| Msa0624510 | Msa1224840 | 0.816302 | 5.932132e-52 | -8.615850e-47 |
| Msa0624510 | Msa1252190 | 0.802440 | 5.605238e-49 | -8.615850e-47 |
| Msa0624510 | Msa1292830 | 0.803902 | 2.793357e-49 | -8.615850e-47 |
| Msa0624510 | Msa1351760 | 0.825267 | 5.145512e-54 | -8.615850e-47 |
| Msa0624510 | Msa1374550 | 0.802414 | 5.673064e-49 | -8.615850e-47 |
| Msa0624510 | Msa1430240 | 0.816861 | 4.445473e-52 | -8.615850e-47 |
| Msa0624510 | Msa1438740 | 0.928081 | 4.779985e-92 | -8.615850e-47 |
| Msa0124340 | Msa0624510 | 0.829899 | 3.976098e-55 | -8.615850e-47 |
| Msa0130570 | Msa0624510 | 0.808442 | 3.091517e-50 | -8.615850e-47 |
| Msa0138820 | Msa0624510 | -0.814752 | 1.312625e-51 | -8.615850e-47 |
| Msa0148100 | Msa0624510 | 0.801206 | 1.004571e-48 | -8.615850e-47 |
| Msa0157290 | Msa0624510 | 0.817638 | 2.972304e-52 | -8.615850e-47 |
| Msa0173890 | Msa0624510 | 0.807438 | 5.054473e-50 | -8.615850e-47 |
| Msa0183830 | Msa0624510 | 0.803665 | 3.127909e-49 | -8.615850e-47 |
| Msa0186890 | Msa0624510 | 0.819361 | 1.209129e-52 | -8.615850e-47 |
| Msa0194540 | Msa0624510 | 0.800558 | 1.361982e-48 | -8.615850e-47 |
| Msa0225740 | Msa0624510 | 0.804689 | 1.914498e-49 | -8.615850e-47 |
| Msa0230550 | Msa0624510 | -0.806443 | 8.204124e-50 | -8.615850e-47 |
| Msa0410500 | Msa0624510 | 0.815032 | 1.137788e-51 | -8.615850e-47 |
| Msa0410650 | Msa0624510 | 0.804885 | 1.742741e-49 | -8.615850e-47 |
| Msa0461720 | Msa0624510 | 0.824232 | 9.023256e-54 | -8.615850e-47 |
| Msa0461730 | Msa0624510 | 0.802485 | 5.486902e-49 | -8.615850e-47 |
| Msa0475790 | Msa0624510 | 0.814907 | 1.212983e-51 | -8.615850e-47 |
| Msa0292400 | Msa0624510 | 0.807823 | 4.187387e-50 | -8.615850e-47 |
| Msa0306300 | Msa0624510 | 0.819776 | 9.723496e-53 | -8.615850e-47 |
| Msa0317280 | Msa0624510 | 0.811126 | 8.181522e-51 | -8.615850e-47 |
| Msa0317340 | Msa0624510 | 0.806069 | 9.839974e-50 | -8.615850e-47 |
| Msa0337540 | Msa0624510 | 0.800577 | 1.350418e-48 | -8.615850e-47 |
| Msa0503560 | Msa0624510 | 0.821547 | 3.810367e-53 | -8.615850e-47 |
| Msa0527550 | Msa0624510 | 0.831650 | 1.479801e-55 | -8.615850e-47 |
| Msa0541860 | Msa0624510 | 0.818049 | 2.400780e-52 | -8.615850e-47 |
| Msa0548660 | Msa0624510 | 0.801130 | 1.041072e-48 | -8.615850e-47 |
| Msa0555910 | Msa0624510 | 0.804276 | 2.334660e-49 | -8.615850e-47 |
| Msa0574890 | Msa0624510 | 0.807981 | 3.874553e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0624510 | MtrunA17_Chr2g0331751 | 93.750 | 208 | 6 | 3 | 1 | 206 | 1 | 203 | 1.39e-137 | 383 |
| Msa0624510 | MtrunA17_Chr4g0004611 | 55.024 | 209 | 79 | 7 | 3 | 206 | 4 | 202 | 1.83e-63 | 195 |
| Msa0624510 | MtrunA17_Chr8g0390541 | 33.175 | 211 | 126 | 6 | 1 | 205 | 1 | 202 | 9.27e-23 | 91.3 |
| Msa0624510 | MtrunA17_Chr6g0485651 | 37.234 | 188 | 92 | 5 | 1 | 182 | 1 | 168 | 1.90e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0624510 | AT3G16070.1 | 32.759 | 174 | 89 | 5 | 9 | 181 | 4 | 150 | 2.78e-20 | 83.6 |
| Msa0624510 | AT1G15260.1 | 35.784 | 204 | 97 | 9 | 5 | 206 | 7 | 178 | 4.60e-20 | 83.6 |
Find 21 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTCAAATGTTTCATCTT+TGG | 0.211526 | 4_3:+2491994 | Msa0624510:CDS |
| CTTTCCTTCAATCCATTCTT+AGG | 0.265660 | 4_3:+2492042 | Msa0624510:CDS |
| TCCACAATATCTAAATAAAT+AGG | 0.347318 | 4_3:-2492432 | None:intergenic |
| GAATTAGAGATGAATGATTC+AGG | 0.387057 | 4_3:+2492348 | Msa0624510:CDS |
| GAATGAAGGTGTTAATGAAT+TGG | 0.390905 | 4_3:+2492311 | Msa0624510:CDS |
| TCTAAGTCAAAGGGAAGATT+TGG | 0.402476 | 4_3:+2492519 | Msa0624510:CDS |
| AGGGAAGATTTGGATCAATC+AGG | 0.432753 | 4_3:+2492529 | Msa0624510:CDS |
| TTCAAATGTTTCATCTTTGG+AGG | 0.433543 | 4_3:+2491997 | Msa0624510:CDS |
| TCCTATTTATTTAGATATTG+TGG | 0.439985 | 4_3:+2492431 | Msa0624510:CDS |
| CTGATCTCAATAGAATAAAC+AGG | 0.442469 | 4_3:+2492136 | Msa0624510:CDS |
| TGAATTGGCATTGAAGATGA+AGG | 0.456133 | 4_3:+2492326 | Msa0624510:CDS |
| GGATTGAAGGAAAGAGATCT+AGG | 0.460419 | 4_3:-2492033 | None:intergenic |
| TTGCTACTTCTACCTAAGAA+TGG | 0.464176 | 4_3:-2492054 | None:intergenic |
| TCTACCTAAGAATGGATTGA+AGG | 0.485195 | 4_3:-2492046 | None:intergenic |
| AAAGGGAATAAGAAGAATGA+AGG | 0.496505 | 4_3:+2492297 | Msa0624510:CDS |
| ATCAAACGCTCTAAGTCAAA+GGG | 0.509484 | 4_3:+2492510 | Msa0624510:CDS |
| CATCAAACGCTCTAAGTCAA+AGG | 0.517132 | 4_3:+2492509 | Msa0624510:CDS |
| CTCAATAGAATAAACAGGAA+TGG | 0.562291 | 4_3:+2492141 | Msa0624510:CDS |
| TCAAATGTTTCATCTTTGGA+GGG | 0.578974 | 4_3:+2491998 | Msa0624510:CDS |
| AGAGCGTTTGATGCTGACAG+AGG | 0.645792 | 4_3:-2492499 | None:intergenic |
| CGTGTCGAGAGAACAAACAA+TGG | 0.658228 | 4_3:+2492179 | Msa0624510:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTTTTAAGTAAAAATT+TGG | - | chr4_3:2491968-2491987 | None:intergenic | 10.0% |
| !!! | TAAAAATTTGGTTTTGCAAA+TGG | - | chr4_3:2491956-2491975 | None:intergenic | 20.0% |
| !! | TCCTATTTATTTAGATATTG+TGG | + | chr4_3:2492431-2492450 | Msa0624510:CDS | 20.0% |
| !! | TCCACAATATCTAAATAAAT+AGG | - | chr4_3:2492435-2492454 | None:intergenic | 20.0% |
| ! | AACTTCAAATGTTTCATCTT+TGG | + | chr4_3:2491994-2492013 | Msa0624510:CDS | 25.0% |
| !!! | TTTTCAGTAAGGTTTTTTTG+TGG | - | chr4_3:2492255-2492274 | None:intergenic | 25.0% |
| ! | CAAAAAAACCTTACTGAAAA+TGG | + | chr4_3:2492255-2492274 | Msa0624510:CDS | 25.0% |
| ! | AGAAAGAGAAAAAAGACAAA+GGG | + | chr4_3:2492280-2492299 | Msa0624510:CDS | 25.0% |
| TTCAAATGTTTCATCTTTGG+AGG | + | chr4_3:2491997-2492016 | Msa0624510:CDS | 30.0% | |
| TCAAATGTTTCATCTTTGGA+GGG | + | chr4_3:2491998-2492017 | Msa0624510:CDS | 30.0% | |
| ! | AAAGAGATCTAGGTAGTTTT+TGG | - | chr4_3:2492026-2492045 | None:intergenic | 30.0% |
| CTGATCTCAATAGAATAAAC+AGG | + | chr4_3:2492136-2492155 | Msa0624510:CDS | 30.0% | |
| CTCAATAGAATAAACAGGAA+TGG | + | chr4_3:2492141-2492160 | Msa0624510:CDS | 30.0% | |
| GAGAAAGAGAAAAAAGACAA+AGG | + | chr4_3:2492279-2492298 | Msa0624510:CDS | 30.0% | |
| AAAGGGAATAAGAAGAATGA+AGG | + | chr4_3:2492297-2492316 | Msa0624510:CDS | 30.0% | |
| !! | GAATGAAGGTGTTAATGAAT+TGG | + | chr4_3:2492311-2492330 | Msa0624510:CDS | 30.0% |
| GAATTAGAGATGAATGATTC+AGG | + | chr4_3:2492348-2492367 | Msa0624510:CDS | 30.0% | |
| CTTTCCTTCAATCCATTCTT+AGG | + | chr4_3:2492042-2492061 | Msa0624510:CDS | 35.0% | |
| !! | TCTACCTAAGAATGGATTGA+AGG | - | chr4_3:2492049-2492068 | None:intergenic | 35.0% |
| ! | TTGCTACTTCTACCTAAGAA+TGG | - | chr4_3:2492057-2492076 | None:intergenic | 35.0% |
| ! | CTTTCTCTCCATTTTCAGTA+AGG | - | chr4_3:2492266-2492285 | None:intergenic | 35.0% |
| ! | TGAATTGGCATTGAAGATGA+AGG | + | chr4_3:2492326-2492345 | Msa0624510:CDS | 35.0% |
| ! | ATCAAACGCTCTAAGTCAAA+GGG | + | chr4_3:2492510-2492529 | Msa0624510:CDS | 35.0% |
| TCTAAGTCAAAGGGAAGATT+TGG | + | chr4_3:2492519-2492538 | Msa0624510:CDS | 35.0% | |
| GGATTGAAGGAAAGAGATCT+AGG | - | chr4_3:2492036-2492055 | None:intergenic | 40.0% | |
| ! | CATCAAACGCTCTAAGTCAA+AGG | + | chr4_3:2492509-2492528 | Msa0624510:CDS | 40.0% |
| AGGGAAGATTTGGATCAATC+AGG | + | chr4_3:2492529-2492548 | Msa0624510:CDS | 40.0% | |
| CGTGTCGAGAGAACAAACAA+TGG | + | chr4_3:2492179-2492198 | Msa0624510:CDS | 45.0% | |
| ! | AGAGCGTTTGATGCTGACAG+AGG | - | chr4_3:2492502-2492521 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 2491937 | 2492557 | 2491937 | ID=Msa0624510;Name=Msa0624510 |
| chr4_3 | mRNA | 2491937 | 2492557 | 2491937 | ID=Msa0624510-mRNA-1;Parent=Msa0624510;Name=Msa0624510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|206 |
| chr4_3 | exon | 2491937 | 2492557 | 2491937 | ID=Msa0624510-mRNA-1:exon:645;Parent=Msa0624510-mRNA-1 |
| chr4_3 | CDS | 2491937 | 2492557 | 2491937 | ID=Msa0624510-mRNA-1:cds;Parent=Msa0624510-mRNA-1 |
| Gene Sequence |
| Protein sequence |
