Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62393.t1 | KEH32124.1 | 91 | 324 | 25 | 1 | 2 | 325 | 13 | 332 | 1.50E-81 | 313.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62393.t1 | Q9XI36 | 64.8 | 71 | 24 | 1 | 3 | 72 | 20 | 90 | 5.2e-19 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62393.t1 | G7JE44 | 91.3 | 323 | 24 | 1 | 3 | 325 | 36 | 354 | 1.1e-81 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene62393 | 0.812215 | 4.74E-51 | -1.69E-46 |
| MS.gene049159 | MS.gene62393 | 0.885847 | 5.71E-72 | -1.69E-46 |
| MS.gene049343 | MS.gene62393 | 0.802891 | 4.52E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene62393 | 0.826608 | 2.47E-54 | -1.69E-46 |
| MS.gene049501 | MS.gene62393 | 0.81672 | 4.78E-52 | -1.69E-46 |
| MS.gene049821 | MS.gene62393 | 0.827063 | 1.92E-54 | -1.69E-46 |
| MS.gene049850 | MS.gene62393 | 0.844083 | 9.41E-59 | -1.69E-46 |
| MS.gene050020 | MS.gene62393 | 0.825031 | 5.85E-54 | -1.69E-46 |
| MS.gene050156 | MS.gene62393 | 0.826052 | 3.35E-54 | -1.69E-46 |
| MS.gene050226 | MS.gene62393 | 0.81395 | 1.97E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene62393 | 0.808092 | 3.67E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene62393 | 0.816099 | 6.58E-52 | -1.69E-46 |
| MS.gene050303 | MS.gene62393 | 0.817535 | 3.14E-52 | -1.69E-46 |
| MS.gene050438 | MS.gene62393 | 0.804962 | 1.68E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene62393 | 0.883766 | 3.40E-71 | -1.69E-46 |
| MS.gene050605 | MS.gene62393 | 0.833369 | 5.55E-56 | -1.69E-46 |
| MS.gene050623 | MS.gene62393 | -0.839921 | 1.19E-57 | -1.69E-46 |
| MS.gene050680 | MS.gene62393 | 0.809877 | 1.52E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene62393 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene62393 | 0.837051 | 6.53E-57 | -1.69E-46 |
| MS.gene050721 | MS.gene62393 | 0.838892 | 2.20E-57 | -1.69E-46 |
| MS.gene050724 | MS.gene62393 | 0.843274 | 1.55E-58 | -1.69E-46 |
| MS.gene050750 | MS.gene62393 | 0.814923 | 1.20E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene62393 | 0.835802 | 1.36E-56 | -1.69E-46 |
| MS.gene050762 | MS.gene62393 | 0.813099 | 3.04E-51 | -1.69E-46 |
| MS.gene050763 | MS.gene62393 | 0.813124 | 3.00E-51 | -1.69E-46 |
| MS.gene050780 | MS.gene62393 | 0.816605 | 5.07E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene62393 | 0.810221 | 1.28E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene62393 | 0.867991 | 9.10E-66 | -1.69E-46 |
| MS.gene050826 | MS.gene62393 | 0.832152 | 1.11E-55 | -1.69E-46 |
| MS.gene050844 | MS.gene62393 | 0.836031 | 1.19E-56 | -1.69E-46 |
| MS.gene050905 | MS.gene62393 | -0.816342 | 5.81E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene62393 | 0.820763 | 5.78E-53 | -1.69E-46 |
| MS.gene050962 | MS.gene62393 | 0.829544 | 4.85E-55 | -1.69E-46 |
| MS.gene051180 | MS.gene62393 | 0.803866 | 2.84E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene62393 | 0.808409 | 3.14E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene62393 | 0.835574 | 1.55E-56 | -1.69E-46 |
| MS.gene051247 | MS.gene62393 | 0.832306 | 1.02E-55 | -1.69E-46 |
| MS.gene051265 | MS.gene62393 | 0.822804 | 1.95E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene62393 | 0.835141 | 1.99E-56 | -1.69E-46 |
| MS.gene051344 | MS.gene62393 | 0.809887 | 1.51E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene62393 | 0.816965 | 4.21E-52 | -1.69E-46 |
| MS.gene052012 | MS.gene62393 | 0.873888 | 1.04E-67 | -1.69E-46 |
| MS.gene052020 | MS.gene62393 | 0.83069 | 2.55E-55 | -1.69E-46 |
| MS.gene052042 | MS.gene62393 | 0.800327 | 1.52E-48 | -1.69E-46 |
| MS.gene05215 | MS.gene62393 | 0.813504 | 2.48E-51 | -1.69E-46 |
| MS.gene052323 | MS.gene62393 | 0.82463 | 7.27E-54 | -1.69E-46 |
| MS.gene052384 | MS.gene62393 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene62393 | 0.816476 | 5.42E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene62393 | 0.824574 | 7.50E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene62393 | 0.829144 | 6.07E-55 | -1.69E-46 |
| MS.gene052611 | MS.gene62393 | 0.83621 | 1.07E-56 | -1.69E-46 |
| MS.gene052665 | MS.gene62393 | 0.80126 | 9.79E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene62393 | 0.834502 | 2.89E-56 | -1.69E-46 |
| MS.gene052863 | MS.gene62393 | 0.872417 | 3.23E-67 | -1.69E-46 |
| MS.gene05288 | MS.gene62393 | 0.817154 | 3.82E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene62393 | 0.853104 | 2.98E-61 | -1.69E-46 |
| MS.gene053006 | MS.gene62393 | 0.801298 | 9.62E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene62393 | 0.894664 | 1.99E-75 | -1.69E-46 |
| MS.gene053074 | MS.gene62393 | 0.825353 | 4.91E-54 | -1.69E-46 |
| MS.gene053166 | MS.gene62393 | 0.825845 | 3.75E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene62393 | 0.840375 | 9.03E-58 | -1.69E-46 |
| MS.gene05414 | MS.gene62393 | 0.819025 | 1.44E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene62393 | 0.824699 | 7.01E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene62393 | 0.836535 | 8.84E-57 | -1.69E-46 |
| MS.gene054379 | MS.gene62393 | 0.810906 | 9.13E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene62393 | 0.815696 | 8.10E-52 | -1.69E-46 |
| MS.gene054433 | MS.gene62393 | 0.863354 | 2.64E-64 | -1.69E-46 |
| MS.gene054467 | MS.gene62393 | 0.831493 | 1.62E-55 | -1.69E-46 |
| MS.gene054472 | MS.gene62393 | 0.861077 | 1.32E-63 | -1.69E-46 |
| MS.gene054559 | MS.gene62393 | 0.818945 | 1.50E-52 | -1.69E-46 |
| MS.gene054596 | MS.gene62393 | 0.800344 | 1.51E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene62393 | 0.81698 | 4.18E-52 | -1.69E-46 |
| MS.gene054658 | MS.gene62393 | 0.836719 | 7.94E-57 | -1.69E-46 |
| MS.gene05468 | MS.gene62393 | 0.833057 | 6.64E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene62393 | 0.837934 | 3.88E-57 | -1.69E-46 |
| MS.gene054994 | MS.gene62393 | 0.825161 | 5.45E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene62393 | 0.842929 | 1.91E-58 | -1.69E-46 |
| MS.gene05517 | MS.gene62393 | 0.85406 | 1.58E-61 | -1.69E-46 |
| MS.gene055222 | MS.gene62393 | 0.824839 | 6.49E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene62393 | 0.808365 | 3.21E-50 | -1.69E-46 |
| MS.gene055310 | MS.gene62393 | 0.800644 | 1.31E-48 | -1.69E-46 |
| MS.gene055313 | MS.gene62393 | 0.802667 | 5.03E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene62393 | 0.815167 | 1.06E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene62393 | 0.816787 | 4.62E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene62393 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene62393 | 0.824426 | 8.12E-54 | -1.69E-46 |
| MS.gene055512 | MS.gene62393 | 0.825362 | 4.88E-54 | -1.69E-46 |
| MS.gene055608 | MS.gene62393 | 0.814301 | 1.65E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene62393 | 0.850195 | 1.99E-60 | -1.69E-46 |
| MS.gene055701 | MS.gene62393 | 0.822308 | 2.54E-53 | -1.69E-46 |
| MS.gene055778 | MS.gene62393 | 0.81045 | 1.15E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene62393 | 0.879995 | 7.90E-70 | -1.69E-46 |
| MS.gene055926 | MS.gene62393 | 0.849037 | 4.18E-60 | -1.69E-46 |
| MS.gene05604 | MS.gene62393 | 0.832172 | 1.10E-55 | -1.69E-46 |
| MS.gene056173 | MS.gene62393 | 0.838826 | 2.28E-57 | -1.69E-46 |
| MS.gene056174 | MS.gene62393 | 0.809887 | 1.51E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene62393 | 0.806874 | 6.66E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene62393 | 0.806454 | 8.16E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene62393 | 0.814826 | 1.26E-51 | -1.69E-46 |
| MS.gene056595 | MS.gene62393 | 0.81659 | 5.11E-52 | -1.69E-46 |
| MS.gene056760 | MS.gene62393 | 0.82647 | 2.67E-54 | -1.69E-46 |
| MS.gene057026 | MS.gene62393 | 0.806312 | 8.75E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene62393 | 0.82278 | 1.97E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene62393 | 0.817377 | 3.40E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene62393 | 0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene62393 | 0.80692 | 6.51E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene62393 | 0.869515 | 2.92E-66 | -1.69E-46 |
| MS.gene057855 | MS.gene62393 | 0.804181 | 2.44E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene62393 | 0.813769 | 2.16E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene62393 | 0.860093 | 2.63E-63 | -1.69E-46 |
| MS.gene058084 | MS.gene62393 | 0.819175 | 1.33E-52 | -1.69E-46 |
| MS.gene058146 | MS.gene62393 | 0.849354 | 3.41E-60 | -1.69E-46 |
| MS.gene058168 | MS.gene62393 | 0.823853 | 1.11E-53 | -1.69E-46 |
| MS.gene058399 | MS.gene62393 | 0.834624 | 2.69E-56 | -1.69E-46 |
| MS.gene05847 | MS.gene62393 | 0.810786 | 9.70E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene62393 | 0.823282 | 1.51E-53 | -1.69E-46 |
| MS.gene058549 | MS.gene62393 | 0.832605 | 8.59E-56 | -1.69E-46 |
| MS.gene058595 | MS.gene62393 | 0.829848 | 4.09E-55 | -1.69E-46 |
| MS.gene058625 | MS.gene62393 | 0.836412 | 9.50E-57 | -1.69E-46 |
| MS.gene058636 | MS.gene62393 | 0.825166 | 5.44E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene62393 | 0.819239 | 1.29E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene62393 | 0.804987 | 1.66E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene62393 | 0.811677 | 6.21E-51 | -1.69E-46 |
| MS.gene059161 | MS.gene62393 | 0.835357 | 1.76E-56 | -1.69E-46 |
| MS.gene059249 | MS.gene62393 | 0.830747 | 2.47E-55 | -1.69E-46 |
| MS.gene059283 | MS.gene62393 | 0.838623 | 2.58E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene62393 | 0.811302 | 7.49E-51 | -1.69E-46 |
| MS.gene059609 | MS.gene62393 | 0.832098 | 1.15E-55 | -1.69E-46 |
| MS.gene059725 | MS.gene62393 | 0.827858 | 1.24E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene62393 | 0.809728 | 1.64E-50 | -1.69E-46 |
| MS.gene05979 | MS.gene62393 | 0.839537 | 1.49E-57 | -1.69E-46 |
| MS.gene059842 | MS.gene62393 | 0.871437 | 6.84E-67 | -1.69E-46 |
| MS.gene059937 | MS.gene62393 | 0.808263 | 3.38E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene62393 | 0.823946 | 1.05E-53 | -1.69E-46 |
| MS.gene060137 | MS.gene62393 | -0.831601 | 1.52E-55 | -1.69E-46 |
| MS.gene060310 | MS.gene62393 | 0.80759 | 4.69E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene62393 | 0.857704 | 1.36E-62 | -1.69E-46 |
| MS.gene060429 | MS.gene62393 | 0.823302 | 1.49E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene62393 | 0.815158 | 1.07E-51 | -1.69E-46 |
| MS.gene060637 | MS.gene62393 | 0.811676 | 6.22E-51 | -1.69E-46 |
| MS.gene060683 | MS.gene62393 | 0.859919 | 2.96E-63 | -1.69E-46 |
| MS.gene060685 | MS.gene62393 | 0.864369 | 1.28E-64 | -1.69E-46 |
| MS.gene060802 | MS.gene62393 | 0.895211 | 1.19E-75 | -1.69E-46 |
| MS.gene060803 | MS.gene62393 | 0.986121 | 9.67E-166 | -1.69E-46 |
| MS.gene060907 | MS.gene62393 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene060998 | MS.gene62393 | 0.842212 | 2.97E-58 | -1.69E-46 |
| MS.gene061040 | MS.gene62393 | -0.808303 | 3.31E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62393.t1 | MTR_4g114770 | 91.049 | 324 | 25 | 1 | 2 | 325 | 13 | 332 | 0.0 | 563 |
| MS.gene62393.t1 | MTR_4g114770 | 90.769 | 325 | 26 | 1 | 1 | 325 | 34 | 354 | 0.0 | 562 |
| MS.gene62393.t1 | MTR_0339s0010 | 91.589 | 321 | 23 | 1 | 5 | 325 | 1 | 317 | 0.0 | 561 |
| MS.gene62393.t1 | MTR_0339s0020 | 88.158 | 304 | 18 | 3 | 1 | 304 | 34 | 319 | 6.70e-165 | 462 |
| MS.gene62393.t1 | MTR_4g114785 | 88.439 | 173 | 16 | 1 | 1 | 173 | 34 | 202 | 9.05e-101 | 295 |
| MS.gene62393.t1 | MTR_6g060845 | 60.748 | 107 | 38 | 2 | 2 | 105 | 19 | 124 | 6.21e-39 | 134 |
| MS.gene62393.t1 | MTR_6g060860 | 56.489 | 131 | 53 | 4 | 3 | 132 | 36 | 163 | 6.80e-39 | 135 |
| MS.gene62393.t1 | MTR_6g060860 | 68.889 | 90 | 27 | 1 | 3 | 92 | 77 | 165 | 1.98e-38 | 135 |
| MS.gene62393.t1 | MTR_6g060845 | 61.321 | 106 | 37 | 2 | 3 | 105 | 36 | 140 | 5.27e-38 | 132 |
| MS.gene62393.t1 | MTR_6g060685 | 67.778 | 90 | 28 | 1 | 3 | 92 | 77 | 165 | 8.77e-37 | 131 |
| MS.gene62393.t1 | MTR_6g052050 | 63.736 | 91 | 31 | 1 | 2 | 92 | 25 | 113 | 9.28e-35 | 123 |
| MS.gene62393.t1 | MTR_4g010210 | 67.470 | 83 | 25 | 2 | 5 | 86 | 3 | 84 | 2.76e-30 | 118 |
| MS.gene62393.t1 | MTR_6g466310 | 51.899 | 79 | 34 | 1 | 34 | 108 | 1 | 79 | 1.03e-17 | 77.4 |
| MS.gene62393.t1 | MTR_6g465040 | 51.899 | 79 | 34 | 1 | 34 | 108 | 1 | 79 | 1.03e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62393.t1 | AT1G15340 | 59.783 | 92 | 34 | 3 | 3 | 93 | 20 | 109 | 3.59e-28 | 112 |
| MS.gene62393.t1 | AT3G15790 | 58.511 | 94 | 35 | 3 | 3 | 94 | 20 | 111 | 2.99e-26 | 105 |
| MS.gene62393.t1 | AT3G15790 | 58.511 | 94 | 35 | 3 | 3 | 94 | 20 | 111 | 2.99e-26 | 105 |
| MS.gene62393.t1 | AT3G15790 | 58.511 | 94 | 35 | 3 | 3 | 94 | 20 | 111 | 2.99e-26 | 105 |
Find 82 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATTCTCGATGATCTTCT+TGG | 0.243354 | 8.2:+13822263 | None:intergenic |
| CAATCAAATTCCGATATGTT+TGG | 0.322423 | 8.2:+13822302 | None:intergenic |
| GACTTTCTGCTACGTTTCTT+AGG | 0.330344 | 8.2:+13822194 | None:intergenic |
| CGGAATTTGATTGGGGCACT+GGG | 0.341266 | 8.2:-13822292 | MS.gene62393:CDS |
| ACGAAACAAGATGGTGGATC+TGG | 0.355890 | 8.2:-13821681 | MS.gene62393:CDS |
| CAAACATATCGGAATTTGAT+TGG | 0.360294 | 8.2:-13822301 | MS.gene62393:CDS |
| GTCTGGAGTTGTTCAGAATA+AGG | 0.361170 | 8.2:-13821892 | MS.gene62393:CDS |
| TCGGAATTTGATTGGGGCAC+TGG | 0.370091 | 8.2:-13822293 | MS.gene62393:CDS |
| TCTTGCTTTGTCTCCTCTGC+TGG | 0.384252 | 8.2:+13821849 | None:intergenic |
| AATCAAATTCCGATATGTTT+GGG | 0.391168 | 8.2:+13822303 | None:intergenic |
| AAACATATCGGAATTTGATT+GGG | 0.399027 | 8.2:-13822300 | MS.gene62393:CDS |
| TCCTGCTTTGTCTCCTCCGC+TGG | 0.406584 | 8.2:+13821771 | None:intergenic |
| AGTAGTTTCTCCAGGTTGAA+TGG | 0.415859 | 8.2:+13822004 | None:intergenic |
| GATCCGTACCAAAAGGCATT+TGG | 0.420195 | 8.2:-13822351 | MS.gene62393:CDS |
| TCTTGTTTCGTCTCTTCCAC+TGG | 0.422030 | 8.2:+13821693 | None:intergenic |
| TCTTGTCGCGTCTTTCTCCT+TGG | 0.423678 | 8.2:+13821551 | None:intergenic |
| AACAGGATCACCCGTGAGGC+TGG | 0.424398 | 8.2:-13821582 | MS.gene62393:CDS |
| ACTTGTTTCGTCTCCTCCAC+TGG | 0.425405 | 8.2:+13821732 | None:intergenic |
| GACAAAGGAGCTGAGGGGTC+TGG | 0.432101 | 8.2:-13821909 | MS.gene62393:CDS |
| ATTGTGTTCACTTCACCAAC+AGG | 0.434194 | 8.2:-13822380 | MS.gene62393:CDS |
| AAAATAAAGAGGAAGATAAC+AGG | 0.438541 | 8.2:-13821599 | MS.gene62393:CDS |
| AATAAGGATGAAGAAAAGAT+TGG | 0.442381 | 8.2:-13821876 | MS.gene62393:CDS |
| CGAAACAAGATGGTGGATCT+GGG | 0.448304 | 8.2:-13821680 | MS.gene62393:CDS |
| GCAAGAAGCTGGCGAAACTA+AGG | 0.450556 | 8.2:-13822111 | MS.gene62393:CDS |
| ACAAAGCAAGAAGAAAAGAT+TGG | 0.458501 | 8.2:-13821837 | MS.gene62393:CDS |
| TAACGGTGGCCCAAACATAT+CGG | 0.461224 | 8.2:-13822312 | MS.gene62393:CDS |
| CAAATCTCCATCAGCAGTGT+TGG | 0.465617 | 8.2:+13821956 | None:intergenic |
| ACTAAGCAAAACGAAAAGAT+TGG | 0.465779 | 8.2:-13821798 | MS.gene62393:CDS |
| TGGTTTCTCCTCCAGCCTCA+CGG | 0.470523 | 8.2:+13821571 | None:intergenic |
| GGTTTCTCCTCCAGCCTCAC+GGG | 0.470641 | 8.2:+13821572 | None:intergenic |
| GAATCAGAGGTGACTGTGAA+TGG | 0.479774 | 8.2:-13821483 | MS.gene62393:CDS |
| GCAATATAAGTAGTTTCTCC+AGG | 0.485009 | 8.2:+13821996 | None:intergenic |
| TGCTGAGAAAACGGCTGAAG+TGG | 0.493970 | 8.2:-13821628 | MS.gene62393:CDS |
| TGGCGAAACTAAGGATGATA+AGG | 0.496710 | 8.2:-13822102 | MS.gene62393:CDS |
| GGGAAATCCAACACTGCTGA+TGG | 0.499797 | 8.2:-13821963 | MS.gene62393:CDS |
| GCCAGCGGAGGAGACAAAGC+AGG | 0.506408 | 8.2:-13821772 | MS.gene62393:CDS |
| GAAAAGTATTTGAAAACTAA+CGG | 0.517809 | 8.2:-13822329 | MS.gene62393:CDS |
| TACTTATATTGCAAAGGATG+AGG | 0.518178 | 8.2:-13821985 | MS.gene62393:CDS |
| GTACGGATCTCCTCTCCTGT+TGG | 0.519310 | 8.2:+13822365 | None:intergenic |
| TGCCCCTACTGCTGAGAAAA+CGG | 0.519959 | 8.2:-13821637 | MS.gene62393:CDS |
| GACGTGGAAATGCAAGAAGC+TGG | 0.536719 | 8.2:-13822122 | MS.gene62393:CDS |
| GGATGATAAGGATGTAGAGC+CGG | 0.539111 | 8.2:-13822090 | MS.gene62393:CDS |
| GAGAGGAGATCCGTACCAAA+AGG | 0.541915 | 8.2:-13822358 | MS.gene62393:CDS |
| ACTTATATTGCAAAGGATGA+GGG | 0.544601 | 8.2:-13821984 | MS.gene62393:CDS |
| TAAGGATGTAGAGCCGGAAA+AGG | 0.552217 | 8.2:-13822084 | MS.gene62393:CDS |
| AGAAAAGATCGGACAGCCAG+TGG | 0.556644 | 8.2:-13821709 | MS.gene62393:CDS |
| TTTCTGCTACGTTTCTTAGG+AGG | 0.568448 | 8.2:+13822197 | None:intergenic |
| ATCGACGACAAAGGAGCTGA+GGG | 0.572360 | 8.2:-13821915 | MS.gene62393:CDS |
| TTCAAAGAAAGAAGCTTCTG+AGG | 0.575578 | 8.2:-13822165 | MS.gene62393:CDS |
| AGCTTCTGAGGACGAAGCAG+AGG | 0.581963 | 8.2:-13822153 | MS.gene62393:CDS |
| ATCATCGAGAATTGTTGAGA+AGG | 0.584060 | 8.2:-13822255 | MS.gene62393:CDS |
| AGAAACTACTTATATTGCAA+AGG | 0.585396 | 8.2:-13821991 | MS.gene62393:CDS |
| GTTAAAGAGTCCATTCAACC+TGG | 0.588074 | 8.2:-13822014 | MS.gene62393:CDS |
| GGAAAAGATTGAACAGCCAG+TGG | 0.588335 | 8.2:-13821748 | MS.gene62393:CDS |
| AACATATCGGAATTTGATTG+GGG | 0.594084 | 8.2:-13822299 | MS.gene62393:CDS |
| GAGAAAACGGCTGAAGTGGA+AGG | 0.605734 | 8.2:-13821624 | MS.gene62393:CDS |
| CTTATATTGCAAAGGATGAG+GGG | 0.609242 | 8.2:-13821983 | MS.gene62393:CDS |
| GTGGAAGAGACGAAACAAGA+TGG | 0.609428 | 8.2:-13821690 | MS.gene62393:CDS |
| ACGAAACAAGTAGAAAAGAT+CGG | 0.611494 | 8.2:-13821720 | MS.gene62393:CDS |
| CGAAAAGATTGGACAGCCAG+CGG | 0.613646 | 8.2:-13821787 | MS.gene62393:CDS |
| AGCGGAGGAGACAAAGCAGG+AGG | 0.614310 | 8.2:-13821769 | MS.gene62393:CDS |
| GCACGATGAAGCAAACTACA+AGG | 0.616620 | 8.2:-13821526 | MS.gene62393:CDS |
| AGTGGAAGGCGAAAATAAAG+AGG | 0.617317 | 8.2:-13821610 | MS.gene62393:CDS |
| TATGAACAAGGCTGAATCAG+AGG | 0.619422 | 8.2:-13821496 | MS.gene62393:CDS |
| AGGATCACCCGTGAGGCTGG+AGG | 0.622567 | 8.2:-13821579 | MS.gene62393:CDS |
| TATCGACGACAAAGGAGCTG+AGG | 0.627859 | 8.2:-13821916 | MS.gene62393:CDS |
| CAAGGTTCATGATATGAACA+AGG | 0.632417 | 8.2:-13821508 | MS.gene62393:CDS |
| AAAGAGAATATCGACGACAA+AGG | 0.633629 | 8.2:-13821924 | MS.gene62393:CDS |
| GTTCACTTCACCAACAGGAG+AGG | 0.638059 | 8.2:-13822375 | MS.gene62393:CDS |
| AGCAGAGGAGACAAAAGACG+TGG | 0.647050 | 8.2:-13822138 | MS.gene62393:CDS |
| AAAGATTGGACTGCCAGCAG+AGG | 0.649175 | 8.2:-13821862 | MS.gene62393:CDS |
| GAAACAAGATGGTGGATCTG+GGG | 0.649794 | 8.2:-13821679 | MS.gene62393:CDS |
| AAAGATTGAACAGCCAGTGG+AGG | 0.649841 | 8.2:-13821745 | MS.gene62393:CDS |
| GAAGAGACGAAACAAGATGG+TGG | 0.653012 | 8.2:-13821687 | MS.gene62393:CDS |
| GAGAATTGTTGAGAAGGTCA+AGG | 0.659236 | 8.2:-13822249 | MS.gene62393:CDS |
| TGAGGCTGGAGGAGAAACCA+AGG | 0.668481 | 8.2:-13821568 | MS.gene62393:CDS |
| AAAGATTGGACAGCCAGCAG+AGG | 0.672505 | 8.2:-13821823 | MS.gene62393:CDS |
| AAAGATTGGACAGCCAGCGG+AGG | 0.683425 | 8.2:-13821784 | MS.gene62393:CDS |
| GGAATTTGATTGGGGCACTG+GGG | 0.689173 | 8.2:-13822291 | MS.gene62393:CDS |
| AAGTATTTGAAAACTAACGG+TGG | 0.726069 | 8.2:-13822326 | MS.gene62393:CDS |
| AGATAACAGGATCACCCGTG+AGG | 0.732047 | 8.2:-13821586 | MS.gene62393:CDS |
| TCGACGACAAAGGAGCTGAG+GGG | 0.744956 | 8.2:-13821914 | MS.gene62393:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAAAATGAAGATAAGAA+GGG | - | chr8.2:13822093-13822112 | MS.gene62393:CDS | 20.0% |
| !! | TAACAAAAATGAAGATAAGA+AGG | - | chr8.2:13822092-13822111 | MS.gene62393:CDS | 20.0% |
| !! | TCTTGTAAAAGTTAATAACT+TGG | - | chr8.2:13821623-13821642 | MS.gene62393:CDS | 20.0% |
| !!! | AATTTTTTGATTAAGAGAAG+AGG | - | chr8.2:13821501-13821520 | MS.gene62393:CDS | 20.0% |
| !!! | GAAAAGTATTTGAAAACTAA+CGG | - | chr8.2:13821817-13821836 | MS.gene62393:CDS | 20.0% |
| !!! | TATTAACTTTTACAAGACTT+TGG | + | chr8.2:13821620-13821639 | None:intergenic | 20.0% |
| !!! | TTTTAATTGAGTATTCTCAA+GGG | - | chr8.2:13821533-13821552 | MS.gene62393:CDS | 20.0% |
| !!! | TTTTTAATTGAGTATTCTCA+AGG | - | chr8.2:13821532-13821551 | MS.gene62393:CDS | 20.0% |
| ! | AAAATAAAGAGGAAGATAAC+AGG | - | chr8.2:13822547-13822566 | MS.gene62393:intron | 25.0% |
| ! | AAACATATCGGAATTTGATT+GGG | - | chr8.2:13821846-13821865 | MS.gene62393:CDS | 25.0% |
| ! | AATAAGGATGAAGAAAAGAT+TGG | - | chr8.2:13822270-13822289 | MS.gene62393:CDS | 25.0% |
| ! | AATCAAATTCCGATATGTTT+GGG | + | chr8.2:13821846-13821865 | None:intergenic | 25.0% |
| ! | AGAAACTACTTATATTGCAA+AGG | - | chr8.2:13822155-13822174 | MS.gene62393:CDS | 25.0% |
| !!! | AACACAATCTCATTTTTCTT+GGG | + | chr8.2:13821754-13821773 | None:intergenic | 25.0% |
| AACATATCGGAATTTGATTG+GGG | - | chr8.2:13821847-13821866 | MS.gene62393:CDS | 30.0% | |
| AAGTATTTGAAAACTAACGG+TGG | - | chr8.2:13821820-13821839 | MS.gene62393:CDS | 30.0% | |
| ACAAAGCAAGAAGAAAAGAT+TGG | - | chr8.2:13822309-13822328 | MS.gene62393:CDS | 30.0% | |
| ACGAAACAAGTAGAAAAGAT+CGG | - | chr8.2:13822426-13822445 | MS.gene62393:intron | 30.0% | |
| ACTAAGCAAAACGAAAAGAT+TGG | - | chr8.2:13822348-13822367 | MS.gene62393:CDS | 30.0% | |
| ACTTATATTGCAAAGGATGA+GGG | - | chr8.2:13822162-13822181 | MS.gene62393:CDS | 30.0% | |
| CAAACATATCGGAATTTGAT+TGG | - | chr8.2:13821845-13821864 | MS.gene62393:CDS | 30.0% | |
| CAATCAAATTCCGATATGTT+TGG | + | chr8.2:13821847-13821866 | None:intergenic | 30.0% | |
| TACTTATATTGCAAAGGATG+AGG | - | chr8.2:13822161-13822180 | MS.gene62393:CDS | 30.0% | |
| ! | ATACTTTTCCAAATGCCTTT+TGG | + | chr8.2:13821806-13821825 | None:intergenic | 30.0% |
| ! | TTGTTAACAACTTCCTTTTC+CGG | + | chr8.2:13822078-13822097 | None:intergenic | 30.0% |
| !! | GAACACAATCTCATTTTTCT+TGG | + | chr8.2:13821755-13821774 | None:intergenic | 30.0% |
| !!! | TTTTTTGGAAAGAACTGCAA+AGG | + | chr8.2:13821721-13821740 | None:intergenic | 30.0% |
| AAAGAGAATATCGACGACAA+AGG | - | chr8.2:13822222-13822241 | MS.gene62393:CDS | 35.0% | |
| ACAATTCTCGATGATCTTCT+TGG | + | chr8.2:13821886-13821905 | None:intergenic | 35.0% | |
| ATCATCGAGAATTGTTGAGA+AGG | - | chr8.2:13821891-13821910 | MS.gene62393:CDS | 35.0% | |
| CAAGGTTCATGATATGAACA+AGG | - | chr8.2:13822638-13822657 | MS.gene62393:intron | 35.0% | |
| CAGTTCTTTCCAAAAAAGTC+AGG | - | chr8.2:13821724-13821743 | MS.gene62393:CDS | 35.0% | |
| CTTATATTGCAAAGGATGAG+GGG | - | chr8.2:13822163-13822182 | MS.gene62393:CDS | 35.0% | |
| GCAATATAAGTAGTTTCTCC+AGG | + | chr8.2:13822153-13822172 | None:intergenic | 35.0% | |
| TTCAAAGAAAGAAGCTTCTG+AGG | - | chr8.2:13821981-13822000 | MS.gene62393:CDS | 35.0% | |
| ! | TTTCCAAATGCCTTTTGGTA+CGG | + | chr8.2:13821801-13821820 | None:intergenic | 35.0% |
| AGTGGAAGGCGAAAATAAAG+AGG | - | chr8.2:13822536-13822555 | MS.gene62393:intron | 40.0% | |
| ATTGTGTTCACTTCACCAAC+AGG | - | chr8.2:13821766-13821785 | MS.gene62393:CDS | 40.0% | |
| GACTTTCTGCTACGTTTCTT+AGG | + | chr8.2:13821955-13821974 | None:intergenic | 40.0% | |
| GAGAATTGTTGAGAAGGTCA+AGG | - | chr8.2:13821897-13821916 | MS.gene62393:CDS | 40.0% | |
| GTTAAAGAGTCCATTCAACC+TGG | - | chr8.2:13822132-13822151 | MS.gene62393:CDS | 40.0% | |
| TATGAACAAGGCTGAATCAG+AGG | - | chr8.2:13822650-13822669 | MS.gene62393:intron | 40.0% | |
| TGGCGAAACTAAGGATGATA+AGG | - | chr8.2:13822044-13822063 | MS.gene62393:CDS | 40.0% | |
| TTTCTGCTACGTTTCTTAGG+AGG | + | chr8.2:13821952-13821971 | None:intergenic | 40.0% | |
| ! | AGTAGTTTCTCCAGGTTGAA+TGG | + | chr8.2:13822145-13822164 | None:intergenic | 40.0% |
| ! | GTCTGGAGTTGTTCAGAATA+AGG | - | chr8.2:13822254-13822273 | MS.gene62393:CDS | 40.0% |
| !! | TTCTTAGGAGGCTCACTTTT+TGG | + | chr8.2:13821940-13821959 | None:intergenic | 40.0% |
| !!! | TTGGGTGTTCCTGACTTTTT+TGG | + | chr8.2:13821736-13821755 | None:intergenic | 40.0% |
| AAAGATTGAACAGCCAGTGG+AGG | - | chr8.2:13822401-13822420 | MS.gene62393:CDS | 45.0% | |
| CAAATCTCCATCAGCAGTGT+TGG | + | chr8.2:13822193-13822212 | None:intergenic | 45.0% | |
| GAAGAGACGAAACAAGATGG+TGG | - | chr8.2:13822459-13822478 | MS.gene62393:intron | 45.0% | |
| GAATCAGAGGTGACTGTGAA+TGG | - | chr8.2:13822663-13822682 | MS.gene62393:intron | 45.0% | |
| GCACGATGAAGCAAACTACA+AGG | - | chr8.2:13822620-13822639 | MS.gene62393:intron | 45.0% | |
| GGAAAAGATTGAACAGCCAG+TGG | - | chr8.2:13822398-13822417 | MS.gene62393:CDS | 45.0% | |
| GGATGATAAGGATGTAGAGC+CGG | - | chr8.2:13822056-13822075 | MS.gene62393:CDS | 45.0% | |
| GTGGAAGAGACGAAACAAGA+TGG | - | chr8.2:13822456-13822475 | MS.gene62393:intron | 45.0% | |
| TAACGGTGGCCCAAACATAT+CGG | - | chr8.2:13821834-13821853 | MS.gene62393:CDS | 45.0% | |
| TAAGGATGTAGAGCCGGAAA+AGG | - | chr8.2:13822062-13822081 | MS.gene62393:CDS | 45.0% | |
| ! | TCTTGTTTCGTCTCTTCCAC+TGG | + | chr8.2:13822456-13822475 | None:intergenic | 45.0% |
| ! | TTTTGCTTAGTCTCCTCTGC+TGG | + | chr8.2:13822339-13822358 | None:intergenic | 45.0% |
| !! | ACGAAACAAGATGGTGGATC+TGG | - | chr8.2:13822465-13822484 | MS.gene62393:intron | 45.0% |
| !! | CGAAACAAGATGGTGGATCT+GGG | - | chr8.2:13822466-13822485 | MS.gene62393:intron | 45.0% |
| !! | GAAACAAGATGGTGGATCTG+GGG | - | chr8.2:13822467-13822486 | MS.gene62393:intron | 45.0% |
| !! | GATCCGTACCAAAAGGCATT+TGG | - | chr8.2:13821795-13821814 | MS.gene62393:CDS | 45.0% |
| AAAGATTGGACAGCCAGCAG+AGG | - | chr8.2:13822323-13822342 | MS.gene62393:CDS | 50.0% | |
| AAAGATTGGACTGCCAGCAG+AGG | - | chr8.2:13822284-13822303 | MS.gene62393:CDS | 50.0% | |
| ACTTGTTTCGTCTCCTCCAC+TGG | + | chr8.2:13822417-13822436 | None:intergenic | 50.0% | |
| AGAAAAGATCGGACAGCCAG+TGG | - | chr8.2:13822437-13822456 | MS.gene62393:intron | 50.0% | |
| AGATAACAGGATCACCCGTG+AGG | - | chr8.2:13822560-13822579 | MS.gene62393:intron | 50.0% | |
| AGCAGAGGAGACAAAAGACG+TGG | - | chr8.2:13822008-13822027 | MS.gene62393:CDS | 50.0% | |
| ATCGACGACAAAGGAGCTGA+GGG | - | chr8.2:13822231-13822250 | MS.gene62393:CDS | 50.0% | |
| CGAAAAGATTGGACAGCCAG+CGG | - | chr8.2:13822359-13822378 | MS.gene62393:CDS | 50.0% | |
| GACGTGGAAATGCAAGAAGC+TGG | - | chr8.2:13822024-13822043 | MS.gene62393:CDS | 50.0% | |
| GAGAAAACGGCTGAAGTGGA+AGG | - | chr8.2:13822522-13822541 | MS.gene62393:intron | 50.0% | |
| GAGAGGAGATCCGTACCAAA+AGG | - | chr8.2:13821788-13821807 | MS.gene62393:CDS | 50.0% | |
| GCAAGAAGCTGGCGAAACTA+AGG | - | chr8.2:13822035-13822054 | MS.gene62393:CDS | 50.0% | |
| GGGAAATCCAACACTGCTGA+TGG | - | chr8.2:13822183-13822202 | MS.gene62393:CDS | 50.0% | |
| GTTCACTTCACCAACAGGAG+AGG | - | chr8.2:13821771-13821790 | MS.gene62393:CDS | 50.0% | |
| TATCGACGACAAAGGAGCTG+AGG | - | chr8.2:13822230-13822249 | MS.gene62393:CDS | 50.0% | |
| TCGGAATTTGATTGGGGCAC+TGG | - | chr8.2:13821853-13821872 | MS.gene62393:CDS | 50.0% | |
| TCTTGCTTTGTCTCCTCTGC+TGG | + | chr8.2:13822300-13822319 | None:intergenic | 50.0% | |
| TCTTGTCGCGTCTTTCTCCT+TGG | + | chr8.2:13822598-13822617 | None:intergenic | 50.0% | |
| TGCCCCTACTGCTGAGAAAA+CGG | - | chr8.2:13822509-13822528 | MS.gene62393:intron | 50.0% | |
| TGCTGAGAAAACGGCTGAAG+TGG | - | chr8.2:13822518-13822537 | MS.gene62393:intron | 50.0% | |
| ! | CGGAATTTGATTGGGGCACT+GGG | - | chr8.2:13821854-13821873 | MS.gene62393:CDS | 50.0% |
| !! | AGCCGTTTTCTCAGCAGTAG+GGG | + | chr8.2:13822514-13822533 | None:intergenic | 50.0% |
| !! | CAGCCGTTTTCTCAGCAGTA+GGG | + | chr8.2:13822515-13822534 | None:intergenic | 50.0% |
| !! | GGAATTTGATTGGGGCACTG+GGG | - | chr8.2:13821855-13821874 | MS.gene62393:CDS | 50.0% |
| !! | TCAGCCGTTTTCTCAGCAGT+AGG | + | chr8.2:13822516-13822535 | None:intergenic | 50.0% |
| !!! | TCACTTTTTGGCTCTGCTGG+AGG | + | chr8.2:13821928-13821947 | None:intergenic | 50.0% |
| AAAGATTGGACAGCCAGCGG+AGG | - | chr8.2:13822362-13822381 | MS.gene62393:CDS | 55.0% | |
| GTACGGATCTCCTCTCCTGT+TGG | + | chr8.2:13821784-13821803 | None:intergenic | 55.0% | |
| TCGACGACAAAGGAGCTGAG+GGG | - | chr8.2:13822232-13822251 | MS.gene62393:CDS | 55.0% | |
| TGAGGCTGGAGGAGAAACCA+AGG | - | chr8.2:13822578-13822597 | MS.gene62393:intron | 55.0% | |
| TGGTTTCTCCTCCAGCCTCA+CGG | + | chr8.2:13822578-13822597 | None:intergenic | 55.0% | |
| ! | AGCTTCTGAGGACGAAGCAG+AGG | - | chr8.2:13821993-13822012 | MS.gene62393:CDS | 55.0% |
| !!! | GGCTCACTTTTTGGCTCTGC+TGG | + | chr8.2:13821931-13821950 | None:intergenic | 55.0% |
| AACAGGATCACCCGTGAGGC+TGG | - | chr8.2:13822564-13822583 | MS.gene62393:intron | 60.0% | |
| AGCGGAGGAGACAAAGCAGG+AGG | - | chr8.2:13822377-13822396 | MS.gene62393:CDS | 60.0% | |
| GACAAAGGAGCTGAGGGGTC+TGG | - | chr8.2:13822237-13822256 | MS.gene62393:CDS | 60.0% | |
| GGTTTCTCCTCCAGCCTCAC+GGG | + | chr8.2:13822577-13822596 | None:intergenic | 60.0% | |
| TCCTGCTTTGTCTCCTCCGC+TGG | + | chr8.2:13822378-13822397 | None:intergenic | 60.0% | |
| AGGATCACCCGTGAGGCTGG+AGG | - | chr8.2:13822567-13822586 | MS.gene62393:intron | 65.0% | |
| GCCAGCGGAGGAGACAAAGC+AGG | - | chr8.2:13822374-13822393 | MS.gene62393:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 13821476 | 13822692 | 13821476 | ID=MS.gene62393 |
| chr8.2 | mRNA | 13821476 | 13822692 | 13821476 | ID=MS.gene62393.t1;Parent=MS.gene62393 |
| chr8.2 | exon | 13822681 | 13822692 | 13822681 | ID=MS.gene62393.t1.exon1;Parent=MS.gene62393.t1 |
| chr8.2 | CDS | 13822681 | 13822692 | 13822681 | ID=cds.MS.gene62393.t1;Parent=MS.gene62393.t1 |
| chr8.2 | exon | 13821476 | 13822441 | 13821476 | ID=MS.gene62393.t1.exon2;Parent=MS.gene62393.t1 |
| chr8.2 | CDS | 13821476 | 13822441 | 13821476 | ID=cds.MS.gene62393.t1;Parent=MS.gene62393.t1 |
| Gene Sequence |
| Protein sequence |
