Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29773.t1 | XP_013454279.1 | 95.6 | 880 | 39 | 0 | 1 | 880 | 1 | 880 | 0.00E+00 | 1470.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29773.t1 | Q9C895 | 52.7 | 903 | 401 | 5 | 1 | 880 | 1 | 900 | 7.3e-217 | 755.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29773.t1 | A0A072UR29 | 95.6 | 880 | 39 | 0 | 1 | 880 | 1 | 880 | 0.0e+00 | 1470.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene29773 | 0.807438 | 5.05E-50 | -1.69E-46 |
| MS.gene049151 | MS.gene29773 | 0.804765 | 1.85E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene29773 | 0.853828 | 1.85E-61 | -1.69E-46 |
| MS.gene049230 | MS.gene29773 | 0.832154 | 1.11E-55 | -1.69E-46 |
| MS.gene049378 | MS.gene29773 | 0.860308 | 2.26E-63 | -1.69E-46 |
| MS.gene049382 | MS.gene29773 | 0.805 | 1.65E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene29773 | 0.84142 | 4.81E-58 | -1.69E-46 |
| MS.gene049501 | MS.gene29773 | 0.819461 | 1.15E-52 | -1.69E-46 |
| MS.gene049647 | MS.gene29773 | 0.80054 | 1.37E-48 | -1.69E-46 |
| MS.gene049669 | MS.gene29773 | 0.805999 | 1.02E-49 | -1.69E-46 |
| MS.gene049756 | MS.gene29773 | 0.807731 | 4.38E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene29773 | 0.866088 | 3.68E-65 | -1.69E-46 |
| MS.gene050227 | MS.gene29773 | 0.870091 | 1.90E-66 | -1.69E-46 |
| MS.gene050228 | MS.gene29773 | 0.878723 | 2.23E-69 | -1.69E-46 |
| MS.gene050442 | MS.gene29773 | 0.862042 | 6.71E-64 | -1.69E-46 |
| MS.gene050605 | MS.gene29773 | 0.8298 | 4.20E-55 | -1.69E-46 |
| MS.gene050623 | MS.gene29773 | -0.900478 | 7.04E-78 | -1.69E-46 |
| MS.gene050642 | MS.gene29773 | 0.846994 | 1.53E-59 | -1.69E-46 |
| MS.gene050680 | MS.gene29773 | 0.822153 | 2.76E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene29773 | 0.844009 | 9.86E-59 | -1.69E-46 |
| MS.gene050721 | MS.gene29773 | 0.843641 | 1.24E-58 | -1.69E-46 |
| MS.gene050724 | MS.gene29773 | 0.835078 | 2.07E-56 | -1.69E-46 |
| MS.gene050750 | MS.gene29773 | 0.802638 | 5.10E-49 | -1.69E-46 |
| MS.gene050759 | MS.gene29773 | 0.81153 | 6.69E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene29773 | 0.848106 | 7.57E-60 | -1.69E-46 |
| MS.gene050763 | MS.gene29773 | 0.847962 | 8.30E-60 | -1.69E-46 |
| MS.gene050780 | MS.gene29773 | 0.842241 | 2.92E-58 | -1.69E-46 |
| MS.gene050815 | MS.gene29773 | 0.801558 | 8.51E-49 | -1.69E-46 |
| MS.gene050826 | MS.gene29773 | 0.801982 | 6.96E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene29773 | 0.803324 | 3.68E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene29773 | 0.81174 | 6.02E-51 | -1.69E-46 |
| MS.gene051210 | MS.gene29773 | 0.825363 | 4.88E-54 | -1.69E-46 |
| MS.gene051243 | MS.gene29773 | 0.802585 | 5.23E-49 | -1.69E-46 |
| MS.gene051452 | MS.gene29773 | 0.80895 | 2.41E-50 | -1.69E-46 |
| MS.gene051535 | MS.gene29773 | 0.817557 | 3.10E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene29773 | 0.815497 | 8.97E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene29773 | 0.856695 | 2.70E-62 | -1.69E-46 |
| MS.gene052012 | MS.gene29773 | 0.854465 | 1.21E-61 | -1.69E-46 |
| MS.gene052020 | MS.gene29773 | 0.813253 | 2.81E-51 | -1.69E-46 |
| MS.gene05215 | MS.gene29773 | 0.821205 | 4.57E-53 | -1.69E-46 |
| MS.gene052176 | MS.gene29773 | 0.809569 | 1.77E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene29773 | 0.82614 | 3.19E-54 | -1.69E-46 |
| MS.gene052333 | MS.gene29773 | 0.816975 | 4.19E-52 | -1.69E-46 |
| MS.gene052384 | MS.gene29773 | 0.812626 | 3.86E-51 | -1.69E-46 |
| MS.gene052426 | MS.gene29773 | 0.821894 | 3.17E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene29773 | 0.824529 | 7.68E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene29773 | 0.809314 | 2.01E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene29773 | 0.852234 | 5.28E-61 | -1.69E-46 |
| MS.gene053074 | MS.gene29773 | 0.817583 | 3.06E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene29773 | 0.828216 | 1.02E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene29773 | 0.805136 | 1.54E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene29773 | 0.821073 | 4.90E-53 | -1.69E-46 |
| MS.gene054184 | MS.gene29773 | 0.802035 | 6.79E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene29773 | 0.807775 | 4.29E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene29773 | 0.802944 | 4.41E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene29773 | -0.809234 | 2.09E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene29773 | 0.842273 | 2.86E-58 | -1.69E-46 |
| MS.gene054433 | MS.gene29773 | 0.804946 | 1.69E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene29773 | 0.81589 | 7.33E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene29773 | 0.831623 | 1.50E-55 | -1.69E-46 |
| MS.gene054472 | MS.gene29773 | 0.816771 | 4.66E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene29773 | 0.819304 | 1.25E-52 | -1.69E-46 |
| MS.gene054559 | MS.gene29773 | 0.823423 | 1.40E-53 | -1.69E-46 |
| MS.gene054596 | MS.gene29773 | 0.807091 | 5.99E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene29773 | 0.823002 | 1.75E-53 | -1.69E-46 |
| MS.gene054658 | MS.gene29773 | 0.837216 | 5.92E-57 | -1.69E-46 |
| MS.gene05468 | MS.gene29773 | 0.82714 | 1.84E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene29773 | 0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene29773 | 0.845764 | 3.31E-59 | -1.69E-46 |
| MS.gene05517 | MS.gene29773 | 0.816761 | 4.68E-52 | -1.69E-46 |
| MS.gene055222 | MS.gene29773 | 0.817393 | 3.38E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene29773 | 0.807478 | 4.96E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene29773 | 0.823547 | 1.31E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene29773 | 0.809874 | 1.53E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene29773 | 0.849336 | 3.45E-60 | -1.69E-46 |
| MS.gene055701 | MS.gene29773 | 0.873388 | 1.53E-67 | -1.69E-46 |
| MS.gene055818 | MS.gene29773 | 0.82726 | 1.73E-54 | -1.69E-46 |
| MS.gene055926 | MS.gene29773 | 0.822553 | 2.23E-53 | -1.69E-46 |
| MS.gene05604 | MS.gene29773 | 0.831058 | 2.07E-55 | -1.69E-46 |
| MS.gene056480 | MS.gene29773 | 0.803061 | 4.17E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene29773 | 0.851844 | 6.81E-61 | -1.69E-46 |
| MS.gene056552 | MS.gene29773 | 0.848911 | 4.53E-60 | -1.69E-46 |
| MS.gene056760 | MS.gene29773 | 0.800876 | 1.17E-48 | -1.69E-46 |
| MS.gene056979 | MS.gene29773 | 0.850813 | 1.33E-60 | -1.69E-46 |
| MS.gene057026 | MS.gene29773 | 0.847201 | 1.34E-59 | -1.69E-46 |
| MS.gene057140 | MS.gene29773 | 0.81468 | 1.36E-51 | -1.69E-46 |
| MS.gene057394 | MS.gene29773 | 0.806886 | 6.61E-50 | -1.69E-46 |
| MS.gene05761 | MS.gene29773 | 0.823964 | 1.04E-53 | -1.69E-46 |
| MS.gene057693 | MS.gene29773 | 0.821274 | 4.40E-53 | -1.69E-46 |
| MS.gene057869 | MS.gene29773 | 0.827349 | 1.64E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene29773 | 0.819806 | 9.57E-53 | -1.69E-46 |
| MS.gene058081 | MS.gene29773 | 0.801403 | 9.16E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene29773 | 0.800005 | 1.77E-48 | -1.69E-46 |
| MS.gene058139 | MS.gene29773 | 0.808638 | 2.81E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene29773 | 0.861828 | 7.80E-64 | -1.69E-46 |
| MS.gene058168 | MS.gene29773 | 0.808542 | 2.94E-50 | -1.69E-46 |
| MS.gene058399 | MS.gene29773 | 0.835539 | 1.58E-56 | -1.69E-46 |
| MS.gene058435 | MS.gene29773 | 0.813292 | 2.76E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene29773 | 0.813536 | 2.44E-51 | -1.69E-46 |
| MS.gene058595 | MS.gene29773 | 0.820396 | 7.01E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene29773 | 0.803435 | 3.49E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene29773 | 0.821549 | 3.81E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene29773 | 0.815833 | 7.55E-52 | -1.69E-46 |
| MS.gene059128 | MS.gene29773 | 0.807267 | 5.49E-50 | -1.69E-46 |
| MS.gene059161 | MS.gene29773 | 0.84571 | 3.43E-59 | -1.69E-46 |
| MS.gene059249 | MS.gene29773 | 0.814573 | 1.44E-51 | -1.69E-46 |
| MS.gene059331 | MS.gene29773 | 0.80366 | 3.14E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene29773 | 0.80433 | 2.27E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene29773 | 0.834636 | 2.67E-56 | -1.69E-46 |
| MS.gene059725 | MS.gene29773 | 0.844358 | 7.94E-59 | -1.69E-46 |
| MS.gene05979 | MS.gene29773 | 0.809666 | 1.69E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene29773 | 0.813129 | 2.99E-51 | -1.69E-46 |
| MS.gene060137 | MS.gene29773 | -0.815608 | 8.47E-52 | -1.69E-46 |
| MS.gene060296 | MS.gene29773 | 0.823605 | 1.27E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene29773 | 0.839878 | 1.22E-57 | -1.69E-46 |
| MS.gene06052 | MS.gene29773 | 0.815361 | 9.62E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene29773 | 0.837291 | 5.67E-57 | -1.69E-46 |
| MS.gene060637 | MS.gene29773 | 0.821014 | 5.06E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene29773 | 0.870708 | 1.19E-66 | -1.69E-46 |
| MS.gene060685 | MS.gene29773 | 0.871744 | 5.41E-67 | -1.69E-46 |
| MS.gene060802 | MS.gene29773 | 0.873314 | 1.62E-67 | -1.69E-46 |
| MS.gene060803 | MS.gene29773 | 0.888645 | 4.91E-73 | -1.69E-46 |
| MS.gene060907 | MS.gene29773 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene060942 | MS.gene29773 | 0.805251 | 1.46E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene29773 | 0.860357 | 2.19E-63 | -1.69E-46 |
| MS.gene061002 | MS.gene29773 | 0.805266 | 1.45E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene29773 | -0.818641 | 1.76E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29773.t1 | MTR_5g085010 | 96.705 | 880 | 29 | 0 | 1 | 880 | 1 | 880 | 0.0 | 1732 |
| MS.gene29773.t1 | MTR_1g068830 | 89.773 | 880 | 90 | 0 | 1 | 880 | 1 | 880 | 0.0 | 1500 |
| MS.gene29773.t1 | MTR_7g046250 | 31.172 | 879 | 597 | 6 | 4 | 880 | 5 | 877 | 4.85e-125 | 399 |
| MS.gene29773.t1 | MTR_7g046250 | 31.844 | 716 | 481 | 5 | 4 | 718 | 5 | 714 | 3.44e-96 | 317 |
| MS.gene29773.t1 | MTR_3g060950 | 73.203 | 153 | 24 | 3 | 711 | 861 | 61 | 198 | 2.28e-60 | 204 |
| MS.gene29773.t1 | MTR_5g065520 | 40.597 | 335 | 97 | 9 | 275 | 593 | 1 | 249 | 1.64e-46 | 172 |
| MS.gene29773.t1 | MTR_8g064230 | 82.143 | 84 | 15 | 0 | 252 | 335 | 2 | 85 | 2.55e-38 | 137 |
| MS.gene29773.t1 | MTR_5g065540 | 85.106 | 47 | 7 | 0 | 47 | 93 | 12 | 58 | 1.75e-19 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29773.t1 | AT1G55250 | 54.649 | 882 | 397 | 3 | 1 | 880 | 1 | 881 | 0.0 | 922 |
| MS.gene29773.t1 | AT1G55250 | 53.437 | 902 | 395 | 6 | 1 | 880 | 1 | 899 | 0.0 | 905 |
| MS.gene29773.t1 | AT1G55250 | 53.156 | 903 | 397 | 5 | 1 | 880 | 1 | 900 | 0.0 | 900 |
| MS.gene29773.t1 | AT1G55250 | 58.421 | 380 | 157 | 1 | 502 | 880 | 4 | 383 | 1.08e-142 | 428 |
| MS.gene29773.t1 | AT1G55250 | 56.345 | 394 | 157 | 2 | 502 | 880 | 4 | 397 | 1.51e-138 | 418 |
| MS.gene29773.t1 | AT2G44950 | 30.499 | 882 | 595 | 11 | 8 | 880 | 6 | 878 | 2.57e-118 | 381 |
| MS.gene29773.t1 | AT2G44950 | 29.701 | 670 | 454 | 10 | 8 | 669 | 6 | 666 | 3.59e-74 | 257 |
Find 159 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCCTCCACAGAATCTTTC+TGG | 0.139617 | 5.3:+68725527 | None:intergenic |
| GATCTTGTATAGCCTGTTTC+TGG | 0.173211 | 5.3:+68726034 | None:intergenic |
| GAAGGAATTGAATTCTAAAT+TGG | 0.246983 | 5.3:-68720111 | MS.gene29773:CDS |
| CAGAATTCAGCTCAGCAATT+TGG | 0.247609 | 5.3:+68715350 | None:intergenic |
| GCTGAACAAGCTGCTGATTC+TGG | 0.251145 | 5.3:+68726067 | None:intergenic |
| TCAACATTGGATTGCTGAAT+TGG | 0.266671 | 5.3:-68720326 | MS.gene29773:CDS |
| TTTGTAGCTGGTTGATGATA+TGG | 0.269147 | 5.3:-68725767 | MS.gene29773:intron |
| CTTTGCGTCGGTTATCTACT+AGG | 0.292056 | 5.3:-68725462 | MS.gene29773:CDS |
| ACCTTTCATTTGCATCAAAC+TGG | 0.292093 | 5.3:+68717742 | None:intergenic |
| GTTACAGATTGAAAAGTTAC+AGG | 0.298097 | 5.3:-68718803 | MS.gene29773:intron |
| GATTGATGATATGACGAAAA+AGG | 0.308345 | 5.3:-68725190 | MS.gene29773:CDS |
| TCAGGAGTTAGAATTTGTTT+TGG | 0.321414 | 5.3:-68718773 | MS.gene29773:CDS |
| AAAAGATTCTTTGAAAACTT+TGG | 0.324595 | 5.3:-68725716 | MS.gene29773:CDS |
| AGCACTCGTCTATATAATTC+TGG | 0.335894 | 5.3:+68725100 | None:intergenic |
| TCTGACATAAAACAACTTAC+AGG | 0.344586 | 5.3:-68725066 | MS.gene29773:intron |
| TTTCTGAGCTTCAAGATTCC+CGG | 0.355151 | 5.3:-68720568 | MS.gene29773:CDS |
| CTTTCAGATTCTCTAACTTC+TGG | 0.356059 | 5.3:+68717909 | None:intergenic |
| CTCTAGACCATGTTCATCTA+AGG | 0.361421 | 5.3:+68717851 | None:intergenic |
| AAAGTTACAGGAGGAGAATC+AGG | 0.371139 | 5.3:-68718791 | MS.gene29773:CDS |
| AAGCTGAAATAGAAGAGTTA+AGG | 0.371256 | 5.3:-68717767 | MS.gene29773:CDS |
| GGAGTTTGCCAGGTGGGAAT+TGG | 0.375620 | 5.3:-68716136 | MS.gene29773:CDS |
| AACCTCGATTTCAGATATAT+AGG | 0.375681 | 5.3:+68717331 | None:intergenic |
| CATCCCCATTTCTTTAGATA+AGG | 0.377185 | 5.3:+68719832 | None:intergenic |
| AGATGAGGCTTCTTCTTGTC+TGG | 0.380963 | 5.3:+68727127 | None:intergenic |
| GCTCTCTCTGCAGGCTTCTC+AGG | 0.381182 | 5.3:+68720734 | None:intergenic |
| CAAAGGATCAACTTCAACAT+TGG | 0.382751 | 5.3:-68720339 | MS.gene29773:CDS |
| TGCAAACAAATGTGCTGAAC+AGG | 0.382951 | 5.3:-68719097 | MS.gene29773:CDS |
| AAAGATATCAAAGCTGAGTT+TGG | 0.385405 | 5.3:-68719869 | MS.gene29773:CDS |
| TTCAGCCTTATCTAAAGAAA+TGG | 0.386599 | 5.3:-68719837 | MS.gene29773:CDS |
| TTCCTTTCCTTCGTGTCCTC+CGG | 0.387864 | 5.3:-68725575 | MS.gene29773:CDS |
| CAGGACTAACATTGCAAAAC+GGG | 0.396999 | 5.3:-68720135 | MS.gene29773:CDS |
| AAAGCAATTTGAGGAACTTC+AGG | 0.401444 | 5.3:-68720525 | MS.gene29773:intron |
| CACTTCCCGAGTATACTCTT+TGG | 0.403272 | 5.3:-68720362 | MS.gene29773:CDS |
| CCTGCCTGTGGAACAGCATT+TGG | 0.404398 | 5.3:-68714834 | MS.gene29773:CDS |
| CAGCCGCCTTGGAGTTTGCC+AGG | 0.406696 | 5.3:-68716146 | MS.gene29773:CDS |
| GTTGATGATATGGCTCTTCT+CGG | 0.410027 | 5.3:-68725757 | MS.gene29773:CDS |
| TCTTCTACTCTCTTCAAGTT+CGG | 0.410715 | 5.3:+68720841 | None:intergenic |
| TCAGCCTTATCTAAAGAAAT+GGG | 0.420197 | 5.3:-68719836 | MS.gene29773:CDS |
| GAAGCTTGAGGAAGAACTAA+TGG | 0.422228 | 5.3:-68715383 | MS.gene29773:CDS |
| AGCTGAAATAGAAGAGTTAA+GGG | 0.422995 | 5.3:-68717766 | MS.gene29773:CDS |
| ACACAGACCAGTATCTTGTT+TGG | 0.429741 | 5.3:+68719968 | None:intergenic |
| TGCTTCCTCCAGGCTGATGC+AGG | 0.438735 | 5.3:-68726102 | MS.gene29773:intron |
| AGATACTGTTGATGATCATA+TGG | 0.440228 | 5.3:-68725419 | MS.gene29773:CDS |
| GCAGACAGAAGTCCAGAAAC+AGG | 0.440620 | 5.3:-68726046 | MS.gene29773:CDS |
| GCTCTTCTCGGGATACAAGC+TGG | 0.449889 | 5.3:-68725745 | MS.gene29773:CDS |
| CTCTTTAGCTTTAATAGCCT+CGG | 0.449984 | 5.3:+68717367 | None:intergenic |
| CAAATGTGCTGAACAGGATT+CGG | 0.450852 | 5.3:-68719091 | MS.gene29773:CDS |
| TCAAGATTTCTTTGTATGCA+TGG | 0.451174 | 5.3:+68714876 | None:intergenic |
| AATATTGACAAAGCAATTTG+AGG | 0.451416 | 5.3:-68720534 | MS.gene29773:CDS |
| ATCTAAAGAAATGGGGATGA+TGG | 0.454064 | 5.3:-68719828 | MS.gene29773:CDS |
| AATTACAAATAGTGAAGAAC+AGG | 0.462184 | 5.3:-68716324 | MS.gene29773:intron |
| GTTCAGCACATTTGTTTGCA+AGG | 0.462913 | 5.3:+68719100 | None:intergenic |
| CGCCTTGGAGTTTGCCAGGT+GGG | 0.462993 | 5.3:-68716142 | MS.gene29773:CDS |
| CGTGAGACGCGGGAAGAAAC+GGG | 0.463036 | 5.3:+68727097 | None:intergenic |
| AATGCAAAAGGATGCAGCTA+TGG | 0.467843 | 5.3:-68720801 | MS.gene29773:CDS |
| TCCGTTCACTGCATCAGCAT+TGG | 0.477019 | 5.3:+68720766 | None:intergenic |
| CCTTTCATTTGCATCAAACT+GGG | 0.477201 | 5.3:+68717743 | None:intergenic |
| TCGTGAGACGCGGGAAGAAA+CGG | 0.486950 | 5.3:+68727096 | None:intergenic |
| CTACATAGAAGTAGATCATC+CGG | 0.493035 | 5.3:+68725556 | None:intergenic |
| TTATATCATGCAGGAGTTCA+CGG | 0.493537 | 5.3:-68715408 | MS.gene29773:intron |
| TTCCACAGGCAGGACATTTG+CGG | 0.497983 | 5.3:+68714844 | None:intergenic |
| GAAGCCTGCAGAGAGAGCTA+TGG | 0.498135 | 5.3:-68720729 | MS.gene29773:CDS |
| ATGGAAAGAATTGAGGATAT+AGG | 0.501506 | 5.3:-68725400 | MS.gene29773:CDS |
| AGGCAGGACATTTGCGGTGA+CGG | 0.501630 | 5.3:+68714850 | None:intergenic |
| CGAGGCTATTAAAGCTAAAG+AGG | 0.502369 | 5.3:-68717366 | MS.gene29773:CDS |
| CTGTCCAAATGCTGTTCCAC+AGG | 0.503225 | 5.3:+68714830 | None:intergenic |
| AAGTACCTTCAGGATTATCA+AGG | 0.503978 | 5.3:+68725689 | None:intergenic |
| CTCTTCTCGGGATACAAGCT+GGG | 0.505460 | 5.3:-68725744 | MS.gene29773:CDS |
| CCTTTGCCAAAGAGTATACT+CGG | 0.508295 | 5.3:+68720356 | None:intergenic |
| AAAACACCTCGCAGCCGCCT+TGG | 0.508645 | 5.3:-68716157 | MS.gene29773:CDS |
| GCAGGACTAACATTGCAAAA+CGG | 0.510569 | 5.3:-68720136 | MS.gene29773:CDS |
| AATCACTTCACGAAAATTGT+CGG | 0.513242 | 5.3:+68725164 | None:intergenic |
| CCCAGTTTGATGCAAATGAA+AGG | 0.514393 | 5.3:-68717743 | MS.gene29773:intron |
| CCAAATGCTGTTCCACAGGC+AGG | 0.515641 | 5.3:+68714834 | None:intergenic |
| GTTCACTGCATCAGCATTGG+AGG | 0.516207 | 5.3:+68720769 | None:intergenic |
| GCCTGGAGGATTATCAGAAA+AGG | 0.516683 | 5.3:-68718741 | MS.gene29773:intron |
| GAAGAGGCCAAACAAGATAC+TGG | 0.517357 | 5.3:-68719975 | MS.gene29773:intron |
| AAAAGGAATTGGATAGTGAA+AGG | 0.522583 | 5.3:-68716020 | MS.gene29773:intron |
| TTCTGAGCTTCAAGATTCCC+GGG | 0.523846 | 5.3:-68720567 | MS.gene29773:CDS |
| ATCATTGACAGAGTCTCTGC+AGG | 0.528509 | 5.3:-68720293 | MS.gene29773:intron |
| AGTCAGTCTGAAAATGCAAA+AGG | 0.529585 | 5.3:-68720813 | MS.gene29773:CDS |
| AAGAATTGAGGATATAGGTC+AGG | 0.532464 | 5.3:-68725395 | MS.gene29773:CDS |
| TGCAAAAGTGTATTAATGAA+AGG | 0.535858 | 5.3:-68720019 | MS.gene29773:CDS |
| TTTGCGTCGGTTATCTACTA+GGG | 0.536181 | 5.3:-68725461 | MS.gene29773:CDS |
| CATATGTATTATACCTTCCG+TGG | 0.537093 | 5.3:+68725354 | None:intergenic |
| AAGCCTGCAGAGAGAGCTAT+GGG | 0.539173 | 5.3:-68720728 | MS.gene29773:CDS |
| AATCAACCAACACTGGGATC+AGG | 0.540393 | 5.3:-68725950 | MS.gene29773:intron |
| TAATTGCAATCAACCAACAC+TGG | 0.541368 | 5.3:-68725957 | MS.gene29773:CDS |
| AAACCCATAGCTCTCTCTGC+AGG | 0.543797 | 5.3:+68720725 | None:intergenic |
| ATGCAAAAGGATGCAGCTAT+GGG | 0.546927 | 5.3:-68720800 | MS.gene29773:CDS |
| TATTAAAGCTAAAGAGGCTG+AGG | 0.549699 | 5.3:-68717360 | MS.gene29773:CDS |
| TCCTAGAGTCAGAAATGTCA+TGG | 0.550226 | 5.3:+68720063 | None:intergenic |
| GGAATTGGCAGATGCTGAGA+AGG | 0.550271 | 5.3:-68716121 | MS.gene29773:CDS |
| TAGATAACCGACGCAAAGCA+AGG | 0.550352 | 5.3:+68725467 | None:intergenic |
| ATCCAGAAAGATTCTGTGGA+GGG | 0.551161 | 5.3:-68725529 | MS.gene29773:CDS |
| GCTTGAATTTCGTGAGACGC+GGG | 0.552167 | 5.3:+68727087 | None:intergenic |
| CTCGGGATACAAGCTGGGAG+AGG | 0.556620 | 5.3:-68725739 | MS.gene29773:CDS |
| TTGATGATATGGCTCTTCTC+GGG | 0.557494 | 5.3:-68725756 | MS.gene29773:CDS |
| AGGAAGCCCTTGCTTTGCGT+CGG | 0.560789 | 5.3:-68725474 | MS.gene29773:CDS |
| CAAAGCTGAGTTTGGTGTGA+TGG | 0.562082 | 5.3:-68719861 | MS.gene29773:CDS |
| TGATCATATGGAAAGAATTG+AGG | 0.563216 | 5.3:-68725407 | MS.gene29773:CDS |
| TCCAATGCTGATGCAGTGAA+CGG | 0.564453 | 5.3:-68720767 | MS.gene29773:CDS |
| GATTACCTTGATAATCCTGA+AGG | 0.565357 | 5.3:-68725694 | MS.gene29773:intron |
| TAACATTGCAAAACGGGAGA+AGG | 0.566402 | 5.3:-68720129 | MS.gene29773:CDS |
| AGCATCTGCCAATTCCCACC+TGG | 0.566723 | 5.3:+68716128 | None:intergenic |
| TCCATGACATTTCTGACTCT+AGG | 0.566902 | 5.3:-68720064 | MS.gene29773:CDS |
| CACGAAGATTTATCCACGGA+AGG | 0.568556 | 5.3:-68725367 | MS.gene29773:intron |
| CGAGATTACTAATTATGTTG+AGG | 0.568617 | 5.3:-68725494 | MS.gene29773:CDS |
| CTGAAGCTGCTTGTGAACAA+AGG | 0.572664 | 5.3:-68717803 | MS.gene29773:CDS |
| GAGGGAGGACTGTAATATCA+AGG | 0.573477 | 5.3:-68717147 | MS.gene29773:intron |
| TTCAATTGAATTCTTCAACT+CGG | 0.577121 | 5.3:+68720700 | None:intergenic |
| CCAACGTCTATTACAGCAAG+TGG | 0.577654 | 5.3:-68717174 | MS.gene29773:CDS |
| CCGAGTATACTCTTTGGCAA+AGG | 0.579298 | 5.3:-68720356 | MS.gene29773:CDS |
| TCTTGAAATCAAGATGGAAG+AGG | 0.579606 | 5.3:-68719991 | MS.gene29773:CDS |
| ATCCGGAGGACACGAAGGAA+AGG | 0.581923 | 5.3:+68725573 | None:intergenic |
| TTCCCACCTGGCAAACTCCA+AGG | 0.583523 | 5.3:+68716140 | None:intergenic |
| ATTACAGCAAGTGGCTGAGA+GGG | 0.584569 | 5.3:-68717165 | MS.gene29773:CDS |
| AATAAAGTCGTCAAAAGCAA+TGG | 0.585648 | 5.3:-68719067 | MS.gene29773:intron |
| GAATGATCTTGAAATCAAGA+TGG | 0.585926 | 5.3:-68719997 | MS.gene29773:CDS |
| TGCTTGAATTTCGTGAGACG+CGG | 0.591340 | 5.3:+68727086 | None:intergenic |
| GAAACGGGAGTGAGAAGATG+AGG | 0.598522 | 5.3:+68727112 | None:intergenic |
| AACCTATATATCTGAAATCG+AGG | 0.599571 | 5.3:-68717333 | MS.gene29773:intron |
| GAATTCAATTGAAGAAGCGA+AGG | 0.600369 | 5.3:-68720690 | MS.gene29773:intron |
| TGGAAACACAGTTGAAGCGA+TGG | 0.602141 | 5.3:-68719808 | MS.gene29773:CDS |
| ATTAATCCAGAAAGATTCTG+TGG | 0.603677 | 5.3:-68725533 | MS.gene29773:CDS |
| CAATAACCTGATCCCAGTGT+TGG | 0.604541 | 5.3:+68725944 | None:intergenic |
| TGGAGTCTGCGGTTAATGCA+AGG | 0.604681 | 5.3:-68720091 | MS.gene29773:CDS |
| AGATAACCGACGCAAAGCAA+GGG | 0.606298 | 5.3:+68725468 | None:intergenic |
| CGAGCAGATTCAGAAAGATG+TGG | 0.606867 | 5.3:-68716052 | MS.gene29773:CDS |
| CACCGCAAATGTCCTGCCTG+TGG | 0.609399 | 5.3:-68714846 | MS.gene29773:CDS |
| AAACACAAGGAGTATACTGT+TGG | 0.609461 | 5.3:-68725126 | MS.gene29773:CDS |
| CTGTTCTGACCGTCCAAAGG+AGG | 0.610548 | 5.3:-68715254 | MS.gene29773:intron |
| TCACTAAGAGAGAAATTGAG+TGG | 0.615962 | 5.3:-68719740 | MS.gene29773:CDS |
| GAATTCTAAATTGGAGTCTG+CGG | 0.618680 | 5.3:-68720102 | MS.gene29773:CDS |
| ACAGCAAGTGGCTGAGAGGG+AGG | 0.618824 | 5.3:-68717162 | MS.gene29773:CDS |
| CCGCCTTGGAGTTTGCCAGG+TGG | 0.620396 | 5.3:-68716143 | MS.gene29773:CDS |
| CACTAAGAGAGAAATTGAGT+GGG | 0.621181 | 5.3:-68719739 | MS.gene29773:CDS |
| CTTTGGACGGTCAGAACAGA+CGG | 0.621473 | 5.3:+68715258 | None:intergenic |
| AAGAGAGAAATTGAGTGGGA+AGG | 0.622883 | 5.3:-68719735 | MS.gene29773:intron |
| AGGAGAGTTTGACGAAATCG+TGG | 0.625770 | 5.3:-68720864 | MS.gene29773:CDS |
| CGTCTGTTCTGACCGTCCAA+AGG | 0.628492 | 5.3:-68715257 | MS.gene29773:CDS |
| AATTGCAATCAACCAACACT+GGG | 0.631357 | 5.3:-68725956 | MS.gene29773:CDS |
| GAGTTCACGGAAGAAGCTTG+AGG | 0.633563 | 5.3:-68715395 | MS.gene29773:CDS |
| GTGAAGGCAAAACATTTACA+TGG | 0.635152 | 5.3:-68716425 | MS.gene29773:CDS |
| CAGAAAGATTCTGTGGAGGG+AGG | 0.638260 | 5.3:-68725526 | MS.gene29773:CDS |
| TTTGGACATGTATGGCCTGG+AGG | 0.642076 | 5.3:-68718755 | MS.gene29773:CDS |
| CAAACTCCAAGGCGGCTGCG+AGG | 0.642342 | 5.3:+68716151 | None:intergenic |
| CAGCCTTATCTAAAGAAATG+GGG | 0.644025 | 5.3:-68719835 | MS.gene29773:CDS |
| AATCCAGAAAGATTCTGTGG+AGG | 0.648780 | 5.3:-68725530 | MS.gene29773:CDS |
| ACAGATTGAAAAGTTACAGG+AGG | 0.653733 | 5.3:-68718800 | MS.gene29773:intron |
| TATTACAGCAAGTGGCTGAG+AGG | 0.658845 | 5.3:-68717166 | MS.gene29773:CDS |
| CCACTTGCTGTAATAGACGT+TGG | 0.660621 | 5.3:+68717174 | None:intergenic |
| AGATCATCCGGAGGACACGA+AGG | 0.660758 | 5.3:+68725568 | None:intergenic |
| TACTCTCCATGCAAAACACA+AGG | 0.662712 | 5.3:-68725139 | MS.gene29773:CDS |
| GCTTGTATCAGAAAGCGTGA+AGG | 0.670954 | 5.3:-68716441 | MS.gene29773:CDS |
| CTTACACGAAGATTTATCCA+CGG | 0.672505 | 5.3:-68725371 | MS.gene29773:CDS |
| CTTTGCCAAAGAGTATACTC+GGG | 0.675113 | 5.3:+68720357 | None:intergenic |
| CATAGAAGTAGATCATCCGG+AGG | 0.734057 | 5.3:+68725559 | None:intergenic |
| CCACCTGGCAAACTCCAAGG+CGG | 0.760540 | 5.3:+68716143 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 68714803 | 68727173 | 68714803 | ID=MS.gene29773 |
| chr5.3 | mRNA | 68714803 | 68727173 | 68714803 | ID=MS.gene29773.t1;Parent=MS.gene29773 |
| chr5.3 | exon | 68727063 | 68727173 | 68727063 | ID=MS.gene29773.t1.exon1;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68727063 | 68727173 | 68727063 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68725951 | 68726112 | 68725951 | ID=MS.gene29773.t1.exon2;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68725951 | 68726112 | 68725951 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68725695 | 68725782 | 68725695 | ID=MS.gene29773.t1.exon3;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68725695 | 68725782 | 68725695 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68725368 | 68725598 | 68725368 | ID=MS.gene29773.t1.exon4;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68725368 | 68725598 | 68725368 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68725067 | 68725234 | 68725067 | ID=MS.gene29773.t1.exon5;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68725067 | 68725234 | 68725067 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68720691 | 68720884 | 68720691 | ID=MS.gene29773.t1.exon6;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68720691 | 68720884 | 68720691 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68720526 | 68720609 | 68720526 | ID=MS.gene29773.t1.exon7;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68720526 | 68720609 | 68720526 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68720294 | 68720413 | 68720294 | ID=MS.gene29773.t1.exon8;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68720294 | 68720413 | 68720294 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68719976 | 68720162 | 68719976 | ID=MS.gene29773.t1.exon9;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68719976 | 68720162 | 68719976 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68719736 | 68719896 | 68719736 | ID=MS.gene29773.t1.exon10;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68719736 | 68719896 | 68719736 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68719068 | 68719139 | 68719068 | ID=MS.gene29773.t1.exon11;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68719068 | 68719139 | 68719068 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68718742 | 68718818 | 68718742 | ID=MS.gene29773.t1.exon12;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68718742 | 68718818 | 68718742 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68717744 | 68717935 | 68717744 | ID=MS.gene29773.t1.exon13;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68717744 | 68717935 | 68717744 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68717334 | 68717406 | 68717334 | ID=MS.gene29773.t1.exon14;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68717334 | 68717406 | 68717334 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68717148 | 68717234 | 68717148 | ID=MS.gene29773.t1.exon15;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68717148 | 68717234 | 68717148 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68716325 | 68716462 | 68716325 | ID=MS.gene29773.t1.exon16;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68716325 | 68716462 | 68716325 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68716021 | 68716223 | 68716021 | ID=MS.gene29773.t1.exon17;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68716021 | 68716223 | 68716021 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68715255 | 68715417 | 68715255 | ID=MS.gene29773.t1.exon18;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68715255 | 68715417 | 68715255 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| chr5.3 | exon | 68714803 | 68714934 | 68714803 | ID=MS.gene29773.t1.exon19;Parent=MS.gene29773.t1 |
| chr5.3 | CDS | 68714803 | 68714934 | 68714803 | ID=cds.MS.gene29773.t1;Parent=MS.gene29773.t1 |
| Gene Sequence |
| Protein sequence |
