AlfalfaGEDB | MS.gene64920

M. sativa cultivar XinJiangDaYe / MS.gene64920

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64920.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64920.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64920.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene64920	0.852156	5.55E-61	-1.69E-46
MS.gene050277	MS.gene64920	0.818773	1.65E-52	-1.69E-46
MS.gene050838	MS.gene64920	0.837528	4.93E-57	-1.69E-46
MS.gene051063	MS.gene64920	0.815235	1.03E-51	-1.69E-46
MS.gene051233	MS.gene64920	0.812606	3.89E-51	-1.69E-46
MS.gene051676	MS.gene64920	0.801489	8.79E-49	-1.69E-46
MS.gene051700	MS.gene64920	0.807156	5.80E-50	-1.69E-46
MS.gene051746	MS.gene64920	0.817018	4.10E-52	-1.69E-46
MS.gene051748	MS.gene64920	0.825869	3.71E-54	-1.69E-46
MS.gene051903	MS.gene64920	0.832006	1.21E-55	-1.69E-46
MS.gene052133	MS.gene64920	0.822733	2.02E-53	-1.69E-46
MS.gene052947	MS.gene64920	0.841043	6.04E-58	-1.69E-46
MS.gene053109	MS.gene64920	0.807814	4.21E-50	-1.69E-46
MS.gene053564	MS.gene64920	0.801945	7.09E-49	-1.69E-46
MS.gene053952	MS.gene64920	0.803039	4.22E-49	-1.69E-46
MS.gene05469	MS.gene64920	0.803395	3.56E-49	-1.69E-46
MS.gene055311	MS.gene64920	0.806932	6.47E-50	-1.69E-46
MS.gene055424	MS.gene64920	0.80905	2.29E-50	-1.69E-46
MS.gene055591	MS.gene64920	-0.808419	3.13E-50	-1.69E-46
MS.gene056215	MS.gene64920	0.809146	2.19E-50	-1.69E-46
MS.gene056580	MS.gene64920	0.830663	2.59E-55	-1.69E-46
MS.gene056631	MS.gene64920	0.819391	1.19E-52	-1.69E-46
MS.gene057057	MS.gene64920	0.855835	4.83E-62	-1.69E-46
MS.gene057094	MS.gene64920	0.82724	1.75E-54	-1.69E-46
MS.gene057234	MS.gene64920	0.833946	3.98E-56	-1.69E-46
MS.gene057705	MS.gene64920	0.823915	1.07E-53	-1.69E-46
MS.gene058055	MS.gene64920	0.810259	1.26E-50	-1.69E-46
MS.gene058074	MS.gene64920	0.814203	1.74E-51	-1.69E-46
MS.gene058238	MS.gene64920	0.813868	2.06E-51	-1.69E-46
MS.gene058906	MS.gene64920	0.820884	5.42E-53	-1.69E-46
MS.gene059171	MS.gene64920	0.804922	1.71E-49	-1.69E-46
MS.gene059898	MS.gene64920	0.80967	1.69E-50	-1.69E-46
MS.gene06001	MS.gene64920	0.855768	5.05E-62	-1.69E-46
MS.gene060463	MS.gene64920	0.809934	1.48E-50	-1.69E-46
MS.gene060465	MS.gene64920	0.814473	1.51E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene64920	MS.gene004124	PPI
MS.gene88680	MS.gene64920	PPI
MS.gene070241	MS.gene64920	PPI
MS.gene64920	MS.gene072493	PPI
MS.gene056215	MS.gene64920	PPI
MS.gene64920	MS.gene04052	PPI
MS.gene64920	MS.gene84536	PPI
MS.gene64920	MS.gene035896	PPI
MS.gene64920	MS.gene061866	PPI
MS.gene64920	MS.gene64650	PPI
MS.gene061876	MS.gene64920	PPI
MS.gene64920	MS.gene048705	PPI
MS.gene64920	MS.gene061874	PPI
MS.gene64920	MS.gene061869	PPI
MS.gene060463	MS.gene64920	PPI
MS.gene64920	MS.gene04972	PPI
MS.gene64920	MS.gene004121	PPI
MS.gene30920	MS.gene64920	PPI
MS.gene64920	MS.gene061867	PPI
MS.gene64920	MS.gene04064	PPI
MS.gene08053	MS.gene64920	PPI
MS.gene64920	MS.gene008276	PPI
MS.gene64920	MS.gene05619	PPI
MS.gene64920	MS.gene04068	PPI
MS.gene64920	MS.gene05623	PPI
MS.gene64920	MS.gene89488	PPI
MS.gene64920	MS.gene66225	PPI
MS.gene64920	MS.gene70955	PPI
MS.gene64920	MS.gene79195	PPI
MS.gene64920	MS.gene61017	PPI
MS.gene64920	MS.gene04576	PPI
MS.gene012391	MS.gene64920	PPI
MS.gene88681	MS.gene64920	PPI
MS.gene64920	MS.gene09871	PPI
MS.gene64920	MS.gene012390	PPI
MS.gene070244	MS.gene64920	PPI
MS.gene64920	MS.gene08121	PPI
MS.gene64920	MS.gene064557	PPI
MS.gene64920	MS.gene04434	PPI
MS.gene009027	MS.gene64920	PPI
MS.gene64920	MS.gene04452	PPI
MS.gene64920	MS.gene04392	PPI
MS.gene31196	MS.gene64920	PPI
MS.gene64920	MS.gene010219	PPI
MS.gene64920	MS.gene040383	PPI
MS.gene060465	MS.gene64920	PPI
MS.gene64920	MS.gene016624	PPI
MS.gene64920	MS.gene73136	PPI
MS.gene64920	MS.gene049821	PPI
MS.gene64920	MS.gene022946	PPI
MS.gene061870	MS.gene64920	PPI
MS.gene64920	MS.gene035720	PPI
MS.gene035718	MS.gene64920	PPI
MS.gene64920	MS.gene035897	PPI
MS.gene64920	MS.gene049705	PPI
MS.gene071136	MS.gene64920	PPI
MS.gene64920	MS.gene070907	PPI
MS.gene78578	MS.gene64920	PPI
MS.gene64920	MS.gene05608	PPI
MS.gene64920	MS.gene68545	PPI
MS.gene64920	MS.gene070242	PPI
MS.gene038469	MS.gene64920	PPI
MS.gene64920	MS.gene82546	PPI
MS.gene028629	MS.gene64920	PPI
MS.gene64920	MS.gene048746	PPI
MS.gene04433	MS.gene64920	PPI
MS.gene64920	MS.gene60093	PPI
MS.gene64920	MS.gene08122	PPI
MS.gene64920	MS.gene09873	PPI
MS.gene035787	MS.gene64920	PPI
MS.gene04067	MS.gene64920	PPI
MS.gene71215	MS.gene64920	PPI
MS.gene64920	MS.gene022932	PPI
MS.gene89487	MS.gene64920	PPI
MS.gene64920	MS.gene043967	PPI
MS.gene64920	MS.gene010220	PPI
MS.gene035719	MS.gene64920	PPI
MS.gene58854	MS.gene64920	PPI
MS.gene64920	MS.gene62520	PPI
MS.gene64920	MS.gene08053	PPI
MS.gene64920	MS.gene018578	PPI
MS.gene64920	MS.gene035721	PPI
MS.gene061872	MS.gene64920	PPI
MS.gene64920	MS.gene018580	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64920.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64920.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene64920.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64920.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64920.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 38 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAACCCTCTACGGATTT+GGG	0.267091	4.4:-43451338	MS.gene64920:CDS
GGAAGAACCCTCTACGGATT+TGG	0.284984	4.4:-43451339	MS.gene64920:CDS
TGATTTCTTATCAAGATGTC+TGG	0.292016	4.4:-43451636	None:intergenic
CTTCTTGGAGAATGTGATTA+GGG	0.347463	4.4:-43451439	MS.gene64920:CDS
TCTTCTTGGAGAATGTGATT+AGG	0.365922	4.4:-43451440	MS.gene64920:CDS
GGAAAGGGTTTGGGAAAGGG+TGG	0.366216	4.4:-43451600	MS.gene64920:CDS
GGAAAAGGAGGAAAGGGTTT+GGG	0.378357	4.4:-43451609	MS.gene64920:CDS
TATCAGTGGTTTGATTTATG+AGG	0.387367	4.4:-43451484	MS.gene64920:CDS
TGGAAAAGGAGGAAAGGGTT+TGG	0.387838	4.4:-43451610	MS.gene64920:CDS
TGGTGTGTTGAAGATCTTCT+TGG	0.431516	4.4:-43451454	MS.gene64920:CDS
AAGATGTCTGGGCGTGGAAA+AGG	0.434173	4.4:-43451624	MS.gene64920:CDS
GGAGGAAAGGGTTTGGGAAA+GGG	0.445686	4.4:-43451603	MS.gene64920:CDS
AGGAGGAAAGGGTTTGGGAA+AGG	0.475381	4.4:-43451604	MS.gene64920:CDS
CTTACACTGAGCACGCTAGA+AGG	0.481731	4.4:-43451407	MS.gene64920:CDS
GACTGTTACCGCCATGGATG+TGG	0.483826	4.4:-43451382	MS.gene64920:CDS
TCGCAGGCTTTGTGATTCCC+TGG	0.493877	4.4:+43451541	None:intergenic
GGTTTATGCTCTCAAGAGAC+AGG	0.499176	4.4:-43451361	MS.gene64920:CDS
GGTGCTTCGTGACAACATCC+AGG	0.512203	4.4:-43451559	MS.gene64920:CDS
GATTTCTTATCAAGATGTCT+GGG	0.518762	4.4:-43451635	None:intergenic
TGTTGTCACGAAGCACCTTA+CGG	0.540622	4.4:+43451565	None:intergenic
CGTCGTCTTGCTCGTAGAGG+AGG	0.557881	4.4:-43451516	MS.gene64920:CDS
AAGAACCCTCTACGGATTTG+GGG	0.565022	4.4:-43451337	MS.gene64920:CDS
TGGAGCAAAACGACACCGTA+AGG	0.575994	4.4:-43451580	MS.gene64920:CDS
CTTATCAAGATGTCTGGGCG+TGG	0.577382	4.4:-43451630	None:intergenic
CGAGCAAGACGACGAATCGC+AGG	0.578319	4.4:+43451525	None:intergenic
GGGCGTGGAAAAGGAGGAAA+GGG	0.587932	4.4:-43451615	MS.gene64920:CDS
AAGGAAGACTGTTACCGCCA+TGG	0.588179	4.4:-43451388	MS.gene64920:CDS
GGAGGAGTGAAGCGTATCAG+TGG	0.591400	4.4:-43451498	MS.gene64920:CDS
ATGTCTGGGCGTGGAAAAGG+AGG	0.593086	4.4:-43451621	MS.gene64920:CDS
AGACAGGGAAGAACCCTCTA+CGG	0.600202	4.4:-43451345	MS.gene64920:CDS
TGGGCGTGGAAAAGGAGGAA+AGG	0.624585	4.4:-43451616	MS.gene64920:CDS
GAGAGCATAAACCACATCCA+TGG	0.625099	4.4:+43451371	None:intergenic
AGAACCCTCTACGGATTTGG+GGG	0.641482	4.4:-43451336	MS.gene64920:CDS
GTGCTTCGTGACAACATCCA+GGG	0.652644	4.4:-43451558	MS.gene64920:CDS
ATTCGTCGTCTTGCTCGTAG+AGG	0.654201	4.4:-43451519	MS.gene64920:CDS
GTTTATGCTCTCAAGAGACA+GGG	0.674567	4.4:-43451360	MS.gene64920:CDS
AGCATAAACCACATCCATGG+CGG	0.701499	4.4:+43451374	None:intergenic
TTGATTTATGAGGAGACACG+TGG	0.706090	4.4:-43451474	MS.gene64920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATCAGTGGTTTGATTTATG+AGG	-	chr4.4:43451469-43451488	MS.gene64920:CDS	30.0%
	CTTCTTGGAGAATGTGATTA+GGG	-	chr4.4:43451514-43451533	MS.gene64920:CDS	35.0%
	TCTTCTTGGAGAATGTGATT+AGG	-	chr4.4:43451513-43451532	MS.gene64920:CDS	35.0%
	GTTTATGCTCTCAAGAGACA+GGG	-	chr4.4:43451593-43451612	MS.gene64920:CDS	40.0%
	TGGTGTGTTGAAGATCTTCT+TGG	-	chr4.4:43451499-43451518	MS.gene64920:CDS	40.0%
	TTGATTTATGAGGAGACACG+TGG	-	chr4.4:43451479-43451498	MS.gene64920:CDS	40.0%
	AAGAACCCTCTACGGATTTG+GGG	-	chr4.4:43451616-43451635	MS.gene64920:CDS	45.0%
	AGCATAAACCACATCCATGG+CGG	+	chr4.4:43451582-43451601	None:intergenic	45.0%
	GAAGAACCCTCTACGGATTT+GGG	-	chr4.4:43451615-43451634	MS.gene64920:CDS	45.0%
	GGAAAAGGAGGAAAGGGTTT+GGG	-	chr4.4:43451344-43451363	MS.gene64920:CDS	45.0%
	GGTTTATGCTCTCAAGAGAC+AGG	-	chr4.4:43451592-43451611	MS.gene64920:CDS	45.0%
	TGGAAAAGGAGGAAAGGGTT+TGG	-	chr4.4:43451343-43451362	MS.gene64920:CDS	45.0%
!	GAGAGCATAAACCACATCCA+TGG	+	chr4.4:43451585-43451604	None:intergenic	45.0%
!	TGTTGTCACGAAGCACCTTA+CGG	+	chr4.4:43451391-43451410	None:intergenic	45.0%
	AAGGAAGACTGTTACCGCCA+TGG	-	chr4.4:43451565-43451584	MS.gene64920:CDS	50.0%
	AGAACCCTCTACGGATTTGG+GGG	-	chr4.4:43451617-43451636	MS.gene64920:CDS	50.0%
	AGACAGGGAAGAACCCTCTA+CGG	-	chr4.4:43451608-43451627	MS.gene64920:CDS	50.0%
	AGGAGGAAAGGGTTTGGGAA+AGG	-	chr4.4:43451349-43451368	MS.gene64920:CDS	50.0%
	ATTCGTCGTCTTGCTCGTAG+AGG	-	chr4.4:43451434-43451453	MS.gene64920:CDS	50.0%
	CTAACCCCCAAATCCGTAGA+GGG	+	chr4.4:43451624-43451643	None:intergenic	50.0%
	CTTACACTGAGCACGCTAGA+AGG	-	chr4.4:43451546-43451565	MS.gene64920:CDS	50.0%
	GGAAGAACCCTCTACGGATT+TGG	-	chr4.4:43451614-43451633	MS.gene64920:CDS	50.0%
	GGAGGAAAGGGTTTGGGAAA+GGG	-	chr4.4:43451350-43451369	MS.gene64920:CDS	50.0%
	GTGCTTCGTGACAACATCCA+GGG	-	chr4.4:43451395-43451414	MS.gene64920:CDS	50.0%
	TGGAGCAAAACGACACCGTA+AGG	-	chr4.4:43451373-43451392	MS.gene64920:CDS	50.0%
	ATGTCTGGGCGTGGAAAAGG+AGG	-	chr4.4:43451332-43451351	MS.gene64920:CDS	55.0%
	GACTGTTACCGCCATGGATG+TGG	-	chr4.4:43451571-43451590	MS.gene64920:CDS	55.0%
	GGAAAGGGTTTGGGAAAGGG+TGG	-	chr4.4:43451353-43451372	MS.gene64920:CDS	55.0%
	GGAGGAGTGAAGCGTATCAG+TGG	-	chr4.4:43451455-43451474	MS.gene64920:CDS	55.0%
	GGGCGTGGAAAAGGAGGAAA+GGG	-	chr4.4:43451338-43451357	MS.gene64920:CDS	55.0%
	GGTGCTTCGTGACAACATCC+AGG	-	chr4.4:43451394-43451413	MS.gene64920:CDS	55.0%
	TGGGCGTGGAAAAGGAGGAA+AGG	-	chr4.4:43451337-43451356	MS.gene64920:CDS	55.0%
!	TCGCAGGCTTTGTGATTCCC+TGG	+	chr4.4:43451415-43451434	None:intergenic	55.0%
	CGAGCAAGACGACGAATCGC+AGG	+	chr4.4:43451431-43451450	None:intergenic	60.0%
	CGTCGTCTTGCTCGTAGAGG+AGG	-	chr4.4:43451437-43451456	MS.gene64920:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	43451332	43451643	43451332	ID=MS.gene64920
chr4.4	mRNA	43451332	43451643	43451332	ID=MS.gene64920.t1;Parent=MS.gene64920
chr4.4	exon	43451332	43451643	43451332	ID=MS.gene64920.t1.exon1;Parent=MS.gene64920.t1
chr4.4	CDS	43451332	43451643	43451332	ID=cds.MS.gene64920.t1;Parent=MS.gene64920.t1

Gene Sequence	>MS.gene64920 ATGTCTGGGCGTGGAAAAGGAGGAAAGGGTTTGGGAAAGGGTGGAGCAAAACGACACCGTAAGGTGCTTCGTGACAACATCCAGGGAATCACAAAGCCTGCGATTCGTCGTCTTGCTCGTAGAGGAGGAGTGAAGCGTATCAGTGGTTTGATTTATGAGGAGACACGTGGTGTGTTGAAGATCTTCTTGGAGAATGTGATTAGGGATGCTGTGACTTACACTGAGCACGCTAGAAGGAAGACTGTTACCGCCATGGATGTGGTTTATGCTCTCAAGAGACAGGGAAGAACCCTCTACGGATTTGGGGGTTAG
Protein sequence	>MS.gene64920.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG