Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09727.t1 | XP_024640428.1 | 97 | 300 | 9 | 0 | 1 | 300 | 23 | 322 | 4.50E-170 | 607.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09727.t1 | O81816 | 32.6 | 310 | 192 | 4 | 2 | 298 | 100 | 405 | 2.4e-42 | 174.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09727.t1 | G7JXH2 | 97.0 | 300 | 9 | 0 | 1 | 300 | 132 | 431 | 3.3e-170 | 607.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene09727 | 0.800047 | 1.73E-48 | -1.69E-46 |
| MS.gene049170 | MS.gene09727 | 0.813292 | 2.76E-51 | -1.69E-46 |
| MS.gene04932 | MS.gene09727 | -0.800351 | 1.50E-48 | -1.69E-46 |
| MS.gene049356 | MS.gene09727 | 0.861806 | 7.92E-64 | -1.69E-46 |
| MS.gene049369 | MS.gene09727 | 0.874212 | 8.05E-68 | -1.69E-46 |
| MS.gene049396 | MS.gene09727 | 0.884863 | 1.33E-71 | -1.69E-46 |
| MS.gene049609 | MS.gene09727 | 0.843556 | 1.30E-58 | -1.69E-46 |
| MS.gene049633 | MS.gene09727 | 0.800526 | 1.38E-48 | -1.69E-46 |
| MS.gene049805 | MS.gene09727 | 0.84408 | 9.43E-59 | -1.69E-46 |
| MS.gene050060 | MS.gene09727 | 0.809741 | 1.63E-50 | -1.69E-46 |
| MS.gene050400 | MS.gene09727 | 0.802628 | 5.13E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene09727 | -0.80273 | 4.88E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene09727 | 0.809974 | 1.45E-50 | -1.69E-46 |
| MS.gene050553 | MS.gene09727 | 0.812756 | 3.61E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene09727 | 0.855826 | 4.86E-62 | -1.69E-46 |
| MS.gene050670 | MS.gene09727 | 0.881585 | 2.12E-70 | -1.69E-46 |
| MS.gene050747 | MS.gene09727 | 0.824898 | 6.29E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene09727 | 0.841835 | 3.73E-58 | -1.69E-46 |
| MS.gene050809 | MS.gene09727 | -0.811484 | 6.84E-51 | -1.69E-46 |
| MS.gene05080 | MS.gene09727 | 0.821136 | 4.74E-53 | -1.69E-46 |
| MS.gene05081 | MS.gene09727 | 0.816179 | 6.32E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene09727 | 0.817459 | 3.26E-52 | -1.69E-46 |
| MS.gene050971 | MS.gene09727 | 0.82528 | 5.11E-54 | -1.69E-46 |
| MS.gene05105 | MS.gene09727 | 0.875079 | 4.09E-68 | -1.69E-46 |
| MS.gene051360 | MS.gene09727 | 0.809904 | 1.50E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene09727 | 0.802956 | 4.39E-49 | -1.69E-46 |
| MS.gene051521 | MS.gene09727 | 0.803198 | 3.91E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene09727 | 0.829825 | 4.14E-55 | -1.69E-46 |
| MS.gene051605 | MS.gene09727 | 0.817632 | 2.98E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene09727 | 0.838944 | 2.13E-57 | -1.69E-46 |
| MS.gene051734 | MS.gene09727 | 0.849905 | 2.39E-60 | -1.69E-46 |
| MS.gene051838 | MS.gene09727 | 0.841055 | 5.99E-58 | -1.69E-46 |
| MS.gene051874 | MS.gene09727 | 0.844008 | 9.86E-59 | -1.69E-46 |
| MS.gene052134 | MS.gene09727 | 0.850558 | 1.57E-60 | -1.69E-46 |
| MS.gene052135 | MS.gene09727 | 0.849072 | 4.09E-60 | -1.69E-46 |
| MS.gene052136 | MS.gene09727 | 0.857807 | 1.27E-62 | -1.69E-46 |
| MS.gene052188 | MS.gene09727 | 0.866326 | 3.10E-65 | -1.69E-46 |
| MS.gene052253 | MS.gene09727 | 0.828638 | 8.04E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene09727 | 0.835473 | 1.64E-56 | -1.69E-46 |
| MS.gene052340 | MS.gene09727 | 0.847297 | 1.26E-59 | -1.69E-46 |
| MS.gene052346 | MS.gene09727 | 0.837241 | 5.84E-57 | -1.69E-46 |
| MS.gene05240 | MS.gene09727 | -0.81624 | 6.12E-52 | -1.69E-46 |
| MS.gene052414 | MS.gene09727 | 0.895716 | 7.36E-76 | -1.69E-46 |
| MS.gene052421 | MS.gene09727 | 0.852375 | 4.81E-61 | -1.69E-46 |
| MS.gene052481 | MS.gene09727 | 0.827117 | 1.87E-54 | -1.69E-46 |
| MS.gene052484 | MS.gene09727 | 0.823741 | 1.18E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene09727 | 0.819376 | 1.20E-52 | -1.69E-46 |
| MS.gene052501 | MS.gene09727 | 0.820332 | 7.25E-53 | -1.69E-46 |
| MS.gene052505 | MS.gene09727 | 0.829078 | 6.30E-55 | -1.69E-46 |
| MS.gene052870 | MS.gene09727 | 0.811067 | 8.43E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene09727 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene09727 | 0.815602 | 8.50E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene09727 | 0.821166 | 4.67E-53 | -1.69E-46 |
| MS.gene053104 | MS.gene09727 | 0.820226 | 7.67E-53 | -1.69E-46 |
| MS.gene053209 | MS.gene09727 | 0.808722 | 2.69E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene09727 | 0.883282 | 5.12E-71 | -1.69E-46 |
| MS.gene053557 | MS.gene09727 | 0.806849 | 6.74E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene09727 | 0.848214 | 7.07E-60 | -1.69E-46 |
| MS.gene053626 | MS.gene09727 | 0.802464 | 5.54E-49 | -1.69E-46 |
| MS.gene053758 | MS.gene09727 | 0.836381 | 9.68E-57 | -1.69E-46 |
| MS.gene053824 | MS.gene09727 | 0.828884 | 7.01E-55 | -1.69E-46 |
| MS.gene053912 | MS.gene09727 | 0.891038 | 5.72E-74 | -1.69E-46 |
| MS.gene053916 | MS.gene09727 | 0.835403 | 1.71E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene09727 | 0.821102 | 4.83E-53 | -1.69E-46 |
| MS.gene054927 | MS.gene09727 | 0.8032 | 3.91E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene09727 | 0.841871 | 3.65E-58 | -1.69E-46 |
| MS.gene054955 | MS.gene09727 | 0.80763 | 4.60E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene09727 | 0.863383 | 2.59E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene09727 | 0.867517 | 1.29E-65 | -1.69E-46 |
| MS.gene055224 | MS.gene09727 | 0.833661 | 4.69E-56 | -1.69E-46 |
| MS.gene055289 | MS.gene09727 | 0.834643 | 2.66E-56 | -1.69E-46 |
| MS.gene055369 | MS.gene09727 | 0.805244 | 1.47E-49 | -1.69E-46 |
| MS.gene055539 | MS.gene09727 | 0.82232 | 2.52E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene09727 | 0.843898 | 1.06E-58 | -1.69E-46 |
| MS.gene055741 | MS.gene09727 | 0.853335 | 2.56E-61 | -1.69E-46 |
| MS.gene055744 | MS.gene09727 | 0.858677 | 6.99E-63 | -1.69E-46 |
| MS.gene055909 | MS.gene09727 | 0.840889 | 6.63E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene09727 | 0.835564 | 1.56E-56 | -1.69E-46 |
| MS.gene05592 | MS.gene09727 | 0.8175 | 3.19E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene09727 | 0.847399 | 1.19E-59 | -1.69E-46 |
| MS.gene056422 | MS.gene09727 | 0.874084 | 8.90E-68 | -1.69E-46 |
| MS.gene056448 | MS.gene09727 | 0.8053 | 1.43E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene09727 | 0.904902 | 7.55E-80 | -1.69E-46 |
| MS.gene056609 | MS.gene09727 | 0.801772 | 7.69E-49 | -1.69E-46 |
| MS.gene05664 | MS.gene09727 | 0.823084 | 1.68E-53 | -1.69E-46 |
| MS.gene05666 | MS.gene09727 | 0.809076 | 2.26E-50 | -1.69E-46 |
| MS.gene056719 | MS.gene09727 | 0.800249 | 1.57E-48 | -1.69E-46 |
| MS.gene056897 | MS.gene09727 | 0.803603 | 3.22E-49 | -1.69E-46 |
| MS.gene057019 | MS.gene09727 | 0.812008 | 5.26E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene09727 | 0.845844 | 3.15E-59 | -1.69E-46 |
| MS.gene057086 | MS.gene09727 | 0.820547 | 6.47E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene09727 | 0.849445 | 3.22E-60 | -1.69E-46 |
| MS.gene057146 | MS.gene09727 | 0.801455 | 8.93E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene09727 | 0.803351 | 3.63E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene09727 | 0.816905 | 4.35E-52 | -1.69E-46 |
| MS.gene05735 | MS.gene09727 | 0.807223 | 5.61E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene09727 | 0.816242 | 6.12E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene09727 | 0.809919 | 1.49E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene09727 | 0.819498 | 1.13E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene09727 | 0.812493 | 4.12E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene09727 | 0.814401 | 1.57E-51 | -1.69E-46 |
| MS.gene057537 | MS.gene09727 | 0.823056 | 1.70E-53 | -1.69E-46 |
| MS.gene057734 | MS.gene09727 | 0.876998 | 8.94E-69 | -1.69E-46 |
| MS.gene057749 | MS.gene09727 | 0.808156 | 3.56E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene09727 | 0.815624 | 8.40E-52 | -1.69E-46 |
| MS.gene057856 | MS.gene09727 | 0.847295 | 1.27E-59 | -1.69E-46 |
| MS.gene057926 | MS.gene09727 | 0.801677 | 8.04E-49 | -1.69E-46 |
| MS.gene057927 | MS.gene09727 | 0.803822 | 2.90E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene09727 | 0.856927 | 2.31E-62 | -1.69E-46 |
| MS.gene058075 | MS.gene09727 | 0.809517 | 1.82E-50 | -1.69E-46 |
| MS.gene058120 | MS.gene09727 | 0.850291 | 1.87E-60 | -1.69E-46 |
| MS.gene058130 | MS.gene09727 | 0.80866 | 2.78E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene09727 | 0.841479 | 4.63E-58 | -1.69E-46 |
| MS.gene058444 | MS.gene09727 | 0.836614 | 8.44E-57 | -1.69E-46 |
| MS.gene058448 | MS.gene09727 | 0.802289 | 6.02E-49 | -1.69E-46 |
| MS.gene058459 | MS.gene09727 | 0.82246 | 2.34E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene09727 | 0.852307 | 5.03E-61 | -1.69E-46 |
| MS.gene058838 | MS.gene09727 | 0.822736 | 2.02E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene09727 | 0.868477 | 6.34E-66 | -1.69E-46 |
| MS.gene058948 | MS.gene09727 | 0.817652 | 2.95E-52 | -1.69E-46 |
| MS.gene05913 | MS.gene09727 | 0.827845 | 1.25E-54 | -1.69E-46 |
| MS.gene059305 | MS.gene09727 | 0.815744 | 7.90E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene09727 | 0.815762 | 7.83E-52 | -1.69E-46 |
| MS.gene059366 | MS.gene09727 | 0.868366 | 6.89E-66 | -1.69E-46 |
| MS.gene059368 | MS.gene09727 | 0.808672 | 2.76E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene09727 | 0.821396 | 4.13E-53 | -1.69E-46 |
| MS.gene059455 | MS.gene09727 | 0.824079 | 9.80E-54 | -1.69E-46 |
| MS.gene059536 | MS.gene09727 | 0.816498 | 5.36E-52 | -1.69E-46 |
| MS.gene059667 | MS.gene09727 | 0.826222 | 3.05E-54 | -1.69E-46 |
| MS.gene059674 | MS.gene09727 | 0.837122 | 6.26E-57 | -1.69E-46 |
| MS.gene059676 | MS.gene09727 | 0.807913 | 4.01E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene09727 | 0.873026 | 2.02E-67 | -1.69E-46 |
| MS.gene059692 | MS.gene09727 | 0.849096 | 4.02E-60 | -1.69E-46 |
| MS.gene059695 | MS.gene09727 | 0.853424 | 2.41E-61 | -1.69E-46 |
| MS.gene059711 | MS.gene09727 | 0.826972 | 2.02E-54 | -1.69E-46 |
| MS.gene059807 | MS.gene09727 | 0.911856 | 3.78E-83 | -1.69E-46 |
| MS.gene059933 | MS.gene09727 | 0.803059 | 4.18E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene09727 | 0.84201 | 3.36E-58 | -1.69E-46 |
| MS.gene060403 | MS.gene09727 | 0.846597 | 1.97E-59 | -1.69E-46 |
| MS.gene060804 | MS.gene09727 | 0.8321 | 1.15E-55 | -1.69E-46 |
| MS.gene060818 | MS.gene09727 | 0.847042 | 1.49E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene09727 | 0.861455 | 1.01E-63 | -1.69E-46 |
| MS.gene060837 | MS.gene09727 | 0.880437 | 5.49E-70 | -1.69E-46 |
| MS.gene06097 | MS.gene09727 | 0.860203 | 2.43E-63 | -1.69E-46 |
| MS.gene060981 | MS.gene09727 | 0.801019 | 1.10E-48 | -1.69E-46 |
| MS.gene061041 | MS.gene09727 | 0.843257 | 1.57E-58 | -1.69E-46 |
| MS.gene061051 | MS.gene09727 | -0.804553 | 2.04E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene09727 | 0.86688 | 2.06E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 96 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATACCTTATTGGAATTCT+TGG | 0.212200 | 5.3:-6148650 | MS.gene09727:CDS |
| ATGTCTAAACAGATTGTAAT+AGG | 0.240740 | 5.3:-6148339 | MS.gene09727:intron |
| TCCAAATGGGGATACCTTAT+TGG | 0.254372 | 5.3:-6148659 | MS.gene09727:CDS |
| TGCCCAAGATTTGGAGTCAT+TGG | 0.307226 | 5.3:+6147816 | None:intergenic |
| CATGCTCCTTGCCCAAGATT+TGG | 0.307845 | 5.3:+6147807 | None:intergenic |
| TTCATTATGTTTAGTAGCTC+TGG | 0.317013 | 5.3:+6147954 | None:intergenic |
| TAGACTTTGGTTGGAGAAAC+AGG | 0.332821 | 5.3:+6148153 | None:intergenic |
| CACCCAATGACTCCAAATCT+TGG | 0.340691 | 5.3:-6147819 | MS.gene09727:CDS |
| AGTGGAGAGGAGGGTACTTT+TGG | 0.353165 | 5.3:-6148752 | MS.gene09727:CDS |
| TCCAATAAGGTATCCCCATT+TGG | 0.359215 | 5.3:+6148658 | None:intergenic |
| TCTCCACTGCACGCACCTCT+TGG | 0.364886 | 5.3:+6148767 | None:intergenic |
| GCCCAAGATTTGGAGTCATT+GGG | 0.380043 | 5.3:+6147817 | None:intergenic |
| CCCATCCAAGAATTCCAATA+AGG | 0.383303 | 5.3:+6148645 | None:intergenic |
| CTAAGGATCTAGTGGAAAAC+TGG | 0.384760 | 5.3:-6147980 | MS.gene09727:CDS |
| GTGAATTACATCTACGGAAA+AGG | 0.386225 | 5.3:-6148195 | MS.gene09727:CDS |
| AGTAAGGCTTGGTCCATTGC+TGG | 0.401844 | 5.3:-6147568 | MS.gene09727:CDS |
| GAAGAATATTGAATGCTATC+AGG | 0.408814 | 5.3:+6148706 | None:intergenic |
| ATAGCATTCAATATTCTTCA+CGG | 0.412492 | 5.3:-6148702 | MS.gene09727:CDS |
| ATGTTTGCCCGTATGGTTAG+TGG | 0.417439 | 5.3:+6147663 | None:intergenic |
| CGTTGGTGGATCGATGGCTT+TGG | 0.417946 | 5.3:-6147899 | MS.gene09727:CDS |
| AAAAGGTTGTCCATCTGAAT+AGG | 0.421657 | 5.3:+6148227 | None:intergenic |
| ACGGGCAAACATTGTGGGTT+CGG | 0.430334 | 5.3:-6147652 | MS.gene09727:CDS |
| ACCCACAATGTTTGCCCGTA+TGG | 0.441085 | 5.3:+6147656 | None:intergenic |
| TGTGGGTTCGGCTTTGCAAT+GGG | 0.445153 | 5.3:-6147640 | MS.gene09727:CDS |
| TTGTCATACCTAAGCTAGTA+AGG | 0.446445 | 5.3:-6147584 | MS.gene09727:CDS |
| ATTTGGAGTCATTGGGTGCC+AGG | 0.447196 | 5.3:+6147824 | None:intergenic |
| AAGATTGAACCAAGTCCAAA+TGG | 0.447760 | 5.3:-6148673 | MS.gene09727:CDS |
| AAGGTATCCCCATTTGGACT+TGG | 0.464282 | 5.3:+6148664 | None:intergenic |
| ACAACTCTACCTGCAGAAAC+AGG | 0.465698 | 5.3:+6147861 | None:intergenic |
| TAATTCACCCGTGCTTCAAA+AGG | 0.468928 | 5.3:+6148210 | None:intergenic |
| ACCTTATTGGAATTCTTGGA+TGG | 0.470171 | 5.3:-6148646 | MS.gene09727:CDS |
| TTTCTCCAACCAAAGTCTAC+TGG | 0.470585 | 5.3:-6148149 | MS.gene09727:CDS |
| TGTCTAAACAGATTGTAATA+GGG | 0.475468 | 5.3:-6148338 | MS.gene09727:intron |
| TGCTTCAACAGTTCATCTCC+AGG | 0.477142 | 5.3:-6148120 | MS.gene09727:intron |
| GGACCAAGCCTTACTAGCTT+AGG | 0.477240 | 5.3:+6147576 | None:intergenic |
| CACCGGATGACACTATAATC+AGG | 0.478910 | 5.3:-6147926 | MS.gene09727:CDS |
| TGAATTACATCTACGGAAAA+GGG | 0.482581 | 5.3:-6148194 | MS.gene09727:CDS |
| ACCCAATGACTCCAAATCTT+GGG | 0.482693 | 5.3:-6147818 | MS.gene09727:CDS |
| ACCATACGGGCAAACATTGT+GGG | 0.483763 | 5.3:-6147657 | MS.gene09727:CDS |
| AGGTGCGTGCAGTGGAGAGG+AGG | 0.485424 | 5.3:-6148762 | MS.gene09727:CDS |
| GCTTGCTTCTTTAGCACCGA+TGG | 0.486635 | 5.3:+6148002 | None:intergenic |
| CTTCTTTAGCACCGATGGCT+CGG | 0.487707 | 5.3:+6148007 | None:intergenic |
| ACCCGCTGGTGTGTTCTGTT+AGG | 0.488168 | 5.3:-6147614 | MS.gene09727:CDS |
| AGATTGAACCAAGTCCAAAT+GGG | 0.490772 | 5.3:-6148672 | MS.gene09727:CDS |
| GCACAATGACCAACAAACTT+TGG | 0.492071 | 5.3:+6148267 | None:intergenic |
| GAGAATATGGCAATGAAAGA+TGG | 0.497368 | 5.3:-6147698 | MS.gene09727:CDS |
| CATTCAATATTCTTCACGGT+TGG | 0.499725 | 5.3:-6148698 | MS.gene09727:CDS |
| TTGTGGGTTCGGCTTTGCAA+TGG | 0.501115 | 5.3:-6147641 | MS.gene09727:CDS |
| GAAACAGGTGGACTTATTGA+TGG | 0.501429 | 5.3:+6147876 | None:intergenic |
| GCAGATAAACCAGTAGACTT+TGG | 0.503117 | 5.3:+6148140 | None:intergenic |
| ATAAACCAGTAGACTTTGGT+TGG | 0.504282 | 5.3:+6148144 | None:intergenic |
| AACCATACGGGCAAACATTG+TGG | 0.523320 | 5.3:-6147658 | MS.gene09727:CDS |
| TTTGCAATGGGACAACCCGC+TGG | 0.523400 | 5.3:-6147628 | MS.gene09727:CDS |
| TATAATCAGGACTCCGTTGG+TGG | 0.527265 | 5.3:-6147913 | MS.gene09727:CDS |
| ATAAGTCCACCTGTTTCTGC+AGG | 0.528684 | 5.3:-6147870 | MS.gene09727:CDS |
| GCATATTTCCACTAACCATA+CGG | 0.529804 | 5.3:-6147671 | MS.gene09727:CDS |
| GTCCTGATTATAGTGTCATC+CGG | 0.533366 | 5.3:+6147924 | None:intergenic |
| TCCTAACAGAACACACCAGC+GGG | 0.539378 | 5.3:+6147613 | None:intergenic |
| CCTTATTGGAATTCTTGGAT+GGG | 0.539919 | 5.3:-6148645 | MS.gene09727:CDS |
| AGGAGCATGTTGTGCTCTTG+AGG | 0.544378 | 5.3:-6147793 | MS.gene09727:CDS |
| GCAGGTAGAGTTGTACTCGT+TGG | 0.545159 | 5.3:-6147852 | MS.gene09727:CDS |
| GAAGCAAGCTAAGGATCTAG+TGG | 0.546205 | 5.3:-6147988 | MS.gene09727:CDS |
| TTGTACTCGTTGGAGACGCC+TGG | 0.546377 | 5.3:-6147842 | MS.gene09727:CDS |
| TCCTAAAATAACCGAGCCAT+CGG | 0.546688 | 5.3:-6148018 | MS.gene09727:CDS |
| AAGAGGTGCGTGCAGTGGAG+AGG | 0.550530 | 5.3:-6148765 | MS.gene09727:CDS |
| CAGCCAAGAGGTGCGTGCAG+TGG | 0.558479 | 5.3:-6148770 | MS.gene09727:CDS |
| ATACCTAAGCTAGTAAGGCT+TGG | 0.561443 | 5.3:-6147579 | MS.gene09727:CDS |
| GGACTCCGTTGGTGGATCGA+TGG | 0.562109 | 5.3:-6147905 | MS.gene09727:CDS |
| AAATTAGTGTGCTCCAGCAA+TGG | 0.570234 | 5.3:+6147555 | None:intergenic |
| GGACTTGCCTCCTATTCAGA+TGG | 0.575892 | 5.3:-6148237 | MS.gene09727:CDS |
| GCACGGGTGAATTACATCTA+CGG | 0.577218 | 5.3:-6148201 | MS.gene09727:CDS |
| TGGGTCTAAACTAGTTGCAA+AGG | 0.577733 | 5.3:-6148626 | MS.gene09727:intron |
| CACTATAATCAGGACTCCGT+TGG | 0.584665 | 5.3:-6147916 | MS.gene09727:CDS |
| ATTATGTTTAGTAGCTCTGG+AGG | 0.585207 | 5.3:+6147957 | None:intergenic |
| AATCCCATCCACTTAGCTAT+CGG | 0.586271 | 5.3:+6148297 | None:intergenic |
| GGTGCGTGCAGTGGAGAGGA+GGG | 0.589579 | 5.3:-6148761 | MS.gene09727:CDS |
| ATCACCGATAGCTAAGTGGA+TGG | 0.589651 | 5.3:-6148301 | MS.gene09727:CDS |
| CATATTTCCACTAACCATAC+GGG | 0.594920 | 5.3:-6147670 | MS.gene09727:CDS |
| ATGACTCCAAATCTTGGGCA+AGG | 0.595031 | 5.3:-6147813 | MS.gene09727:CDS |
| AGGTTGTCCATCTGAATAGG+AGG | 0.597237 | 5.3:+6148230 | None:intergenic |
| TACGGAAAAGGGTTACGCGC+AGG | 0.600680 | 5.3:-6148183 | MS.gene09727:CDS |
| GTTGGTCATTGTGCTTTCCG+TGG | 0.600904 | 5.3:-6148258 | MS.gene09727:CDS |
| CAGATTGTAATAGGGTGCGA+CGG | 0.604784 | 5.3:-6148330 | MS.gene09727:CDS |
| AAACATAATGAATTCTACAC+CGG | 0.605859 | 5.3:-6147943 | MS.gene09727:CDS |
| TTGTGCTCTTGAGGATGCAG+TGG | 0.611787 | 5.3:-6147784 | MS.gene09727:CDS |
| TTCGATCACCGATAGCTAAG+TGG | 0.615756 | 5.3:-6148305 | MS.gene09727:CDS |
| TCACCGATAGCTAAGTGGAT+GGG | 0.628133 | 5.3:-6148300 | MS.gene09727:CDS |
| GGTGCTAAAGAAGCAAGCTA+AGG | 0.629803 | 5.3:-6147997 | MS.gene09727:CDS |
| CAAAGCCATCGATCCACCAA+CGG | 0.639408 | 5.3:+6147900 | None:intergenic |
| GATTGAACCAAGTCCAAATG+GGG | 0.644561 | 5.3:-6148671 | MS.gene09727:CDS |
| ACGGATATTGTGCAGCCAAG+AGG | 0.645003 | 5.3:-6148782 | None:intergenic |
| CTGAATAGGAGGCAAGTCCA+CGG | 0.650582 | 5.3:+6148241 | None:intergenic |
| GAATATTGAATGCTATCAGG+AGG | 0.654073 | 5.3:+6148709 | None:intergenic |
| ACTCTACCTGCAGAAACAGG+TGG | 0.672264 | 5.3:+6147864 | None:intergenic |
| TTCCTAACAGAACACACCAG+CGG | 0.695061 | 5.3:+6147612 | None:intergenic |
| ATGGTTAGTGGAAATATGCG+CGG | 0.713230 | 5.3:+6147675 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTAAATCTAATAAAATCA+TGG | + | chr5.3:6147747-6147766 | None:intergenic | 15.0% |
| !!! | TTTTATTAGATTTACAAACT+TGG | - | chr5.3:6147750-6147769 | MS.gene09727:CDS | 15.0% |
| !!! | TCAATTTGTTAACACTTTTT+AGG | + | chr5.3:6147934-6147953 | None:intergenic | 20.0% |
| ! | AAACATAATGAATTCTACAC+CGG | - | chr5.3:6148354-6148373 | MS.gene09727:intron | 25.0% |
| ! | ATAGCATTCAATATTCTTCA+CGG | - | chr5.3:6147595-6147614 | MS.gene09727:CDS | 25.0% |
| ! | ATGTCTAAACAGATTGTAAT+AGG | - | chr5.3:6147958-6147977 | MS.gene09727:CDS | 25.0% |
| ! | ATTGTAATTTATGGTGTGTT+TGG | - | chr5.3:6147700-6147719 | MS.gene09727:CDS | 25.0% |
| ! | GAATTATGTTTAAACACAGT+CGG | + | chr5.3:6147800-6147819 | None:intergenic | 25.0% |
| ! | GTTACTAATAAGTCCATATA+TGG | - | chr5.3:6148202-6148221 | MS.gene09727:CDS | 25.0% |
| ! | TATTAATCTAACCACATGAT+TGG | - | chr5.3:6147840-6147859 | MS.gene09727:CDS | 25.0% |
| ! | TATTACAATCTGTTTAGACA+TGG | + | chr5.3:6147959-6147978 | None:intergenic | 25.0% |
| ! | TCAGGTAATTTAACCATATA+TGG | + | chr5.3:6148218-6148237 | None:intergenic | 25.0% |
| ! | TGAAAAATAGTTGAGTTCAA+TGG | - | chr5.3:6148252-6148271 | MS.gene09727:CDS | 25.0% |
| ! | TGTCTAAACAGATTGTAATA+GGG | - | chr5.3:6147959-6147978 | MS.gene09727:CDS | 25.0% |
| ! | TTCATATGAACAATAAACTC+AGG | + | chr5.3:6148236-6148255 | None:intergenic | 25.0% |
| !! | AGGTTAGTCATTGTAATTTA+TGG | - | chr5.3:6147691-6147710 | MS.gene09727:CDS | 25.0% |
| AACTTGGTTGACAAAACATT+TGG | - | chr5.3:6147766-6147785 | MS.gene09727:CDS | 30.0% | |
| GAAGAATATTGAATGCTATC+AGG | + | chr5.3:6147594-6147613 | None:intergenic | 30.0% | |
| TGAATTACATCTACGGAAAA+GGG | - | chr5.3:6148103-6148122 | MS.gene09727:intron | 30.0% | |
| TTCATTATGTTTAGTAGCTC+TGG | + | chr5.3:6148346-6148365 | None:intergenic | 30.0% | |
| ! | TTTTGTGCAGTATGTTTTCA+GGG | + | chr5.3:6147872-6147891 | None:intergenic | 30.0% |
| AAAAGGTTGTCCATCTGAAT+AGG | + | chr5.3:6148073-6148092 | None:intergenic | 35.0% | |
| AAATAGTTGAGTTCAATGGC+AGG | - | chr5.3:6148256-6148275 | MS.gene09727:CDS | 35.0% | |
| AAGATTGAACCAAGTCCAAA+TGG | - | chr5.3:6147624-6147643 | MS.gene09727:CDS | 35.0% | |
| ACCTTATTGGAATTCTTGGA+TGG | - | chr5.3:6147651-6147670 | MS.gene09727:CDS | 35.0% | |
| AGATTGAACCAAGTCCAAAT+GGG | - | chr5.3:6147625-6147644 | MS.gene09727:CDS | 35.0% | |
| ATAAACCAGTAGACTTTGGT+TGG | + | chr5.3:6148156-6148175 | None:intergenic | 35.0% | |
| CATATTTCCACTAACCATAC+GGG | - | chr5.3:6148627-6148646 | MS.gene09727:CDS | 35.0% | |
| CATTCAATATTCTTCACGGT+TGG | - | chr5.3:6147599-6147618 | MS.gene09727:CDS | 35.0% | |
| CCTTATTGGAATTCTTGGAT+GGG | - | chr5.3:6147652-6147671 | MS.gene09727:CDS | 35.0% | |
| CTGAAAACATACTGCACAAA+AGG | - | chr5.3:6147871-6147890 | MS.gene09727:CDS | 35.0% | |
| GAATATTGAATGCTATCAGG+AGG | + | chr5.3:6147591-6147610 | None:intergenic | 35.0% | |
| GAGAATATGGCAATGAAAGA+TGG | - | chr5.3:6148599-6148618 | MS.gene09727:intron | 35.0% | |
| GCATATTTCCACTAACCATA+CGG | - | chr5.3:6148626-6148645 | MS.gene09727:intron | 35.0% | |
| GGATACCTTATTGGAATTCT+TGG | - | chr5.3:6147647-6147666 | MS.gene09727:CDS | 35.0% | |
| GTGAATTACATCTACGGAAA+AGG | - | chr5.3:6148102-6148121 | MS.gene09727:intron | 35.0% | |
| TAGGACTAAAGCTTCAGAAT+TGG | + | chr5.3:6147915-6147934 | None:intergenic | 35.0% | |
| TGGACTTATTAGTAACAACC+TGG | + | chr5.3:6148198-6148217 | None:intergenic | 35.0% | |
| TTGTCATACCTAAGCTAGTA+AGG | - | chr5.3:6148713-6148732 | MS.gene09727:CDS | 35.0% | |
| ! | CTTTTGTGCAGTATGTTTTC+AGG | + | chr5.3:6147873-6147892 | None:intergenic | 35.0% |
| ! | TTTTCTGAGCCAAAGTTTGT+TGG | - | chr5.3:6148021-6148040 | MS.gene09727:CDS | 35.0% |
| !! | ATTATGTTTAGTAGCTCTGG+AGG | + | chr5.3:6148343-6148362 | None:intergenic | 35.0% |
| !! | GAAGACGCTTTTAGAGAATA+TGG | - | chr5.3:6148586-6148605 | MS.gene09727:intron | 35.0% |
| AAATTAGTGTGCTCCAGCAA+TGG | + | chr5.3:6148745-6148764 | None:intergenic | 40.0% | |
| AATCCCATCCACTTAGCTAT+CGG | + | chr5.3:6148003-6148022 | None:intergenic | 40.0% | |
| ACCCAATGACTCCAAATCTT+GGG | - | chr5.3:6148479-6148498 | MS.gene09727:intron | 40.0% | |
| ATACCTAAGCTAGTAAGGCT+TGG | - | chr5.3:6148718-6148737 | MS.gene09727:CDS | 40.0% | |
| ATGGTTAGTGGAAATATGCG+CGG | + | chr5.3:6148625-6148644 | None:intergenic | 40.0% | |
| CCCATCCAAGAATTCCAATA+AGG | + | chr5.3:6147655-6147674 | None:intergenic | 40.0% | |
| CTAAGGATCTAGTGGAAAAC+TGG | - | chr5.3:6148317-6148336 | MS.gene09727:CDS | 40.0% | |
| GATTGAACCAAGTCCAAATG+GGG | - | chr5.3:6147626-6147645 | MS.gene09727:CDS | 40.0% | |
| GCACAATGACCAACAAACTT+TGG | + | chr5.3:6148033-6148052 | None:intergenic | 40.0% | |
| GCAGATAAACCAGTAGACTT+TGG | + | chr5.3:6148160-6148179 | None:intergenic | 40.0% | |
| GTCCTGATTATAGTGTCATC+CGG | + | chr5.3:6148376-6148395 | None:intergenic | 40.0% | |
| TAATTCACCCGTGCTTCAAA+AGG | + | chr5.3:6148090-6148109 | None:intergenic | 40.0% | |
| TAGACTTTGGTTGGAGAAAC+AGG | + | chr5.3:6148147-6148166 | None:intergenic | 40.0% | |
| TCCAAATGGGGATACCTTAT+TGG | - | chr5.3:6147638-6147657 | MS.gene09727:CDS | 40.0% | |
| TCCTAAAATAACCGAGCCAT+CGG | - | chr5.3:6148279-6148298 | MS.gene09727:CDS | 40.0% | |
| TGGGTCTAAACTAGTTGCAA+AGG | - | chr5.3:6147671-6147690 | MS.gene09727:CDS | 40.0% | |
| TTTCTCCAACCAAAGTCTAC+TGG | - | chr5.3:6148148-6148167 | MS.gene09727:CDS | 40.0% | |
| ! | GAAACAGGTGGACTTATTGA+TGG | + | chr5.3:6148424-6148443 | None:intergenic | 40.0% |
| ! | TCCAATAAGGTATCCCCATT+TGG | + | chr5.3:6147642-6147661 | None:intergenic | 40.0% |
| !!! | ATGGACAACCTTTTGAAGCA+CGG | - | chr5.3:6148079-6148098 | MS.gene09727:intron | 40.0% |
| !!! | GAGGGTACTTTTGGAAACTT+TGG | - | chr5.3:6147554-6147573 | MS.gene09727:CDS | 40.0% |
| AACCATACGGGCAAACATTG+TGG | - | chr5.3:6148639-6148658 | MS.gene09727:CDS | 45.0% | |
| ACAACTCTACCTGCAGAAAC+AGG | + | chr5.3:6148439-6148458 | None:intergenic | 45.0% | |
| ACCATACGGGCAAACATTGT+GGG | - | chr5.3:6148640-6148659 | MS.gene09727:CDS | 45.0% | |
| AGGTTGTCCATCTGAATAGG+AGG | + | chr5.3:6148070-6148089 | None:intergenic | 45.0% | |
| ATCACCGATAGCTAAGTGGA+TGG | - | chr5.3:6147996-6148015 | MS.gene09727:CDS | 45.0% | |
| ATGACTCCAAATCTTGGGCA+AGG | - | chr5.3:6148484-6148503 | MS.gene09727:intron | 45.0% | |
| ATGTTTGCCCGTATGGTTAG+TGG | + | chr5.3:6148637-6148656 | None:intergenic | 45.0% | |
| CACCCAATGACTCCAAATCT+TGG | - | chr5.3:6148478-6148497 | MS.gene09727:intron | 45.0% | |
| CACCGGATGACACTATAATC+AGG | - | chr5.3:6148371-6148390 | MS.gene09727:intron | 45.0% | |
| CACTATAATCAGGACTCCGT+TGG | - | chr5.3:6148381-6148400 | MS.gene09727:intron | 45.0% | |
| CAGATTGTAATAGGGTGCGA+CGG | - | chr5.3:6147967-6147986 | MS.gene09727:CDS | 45.0% | |
| CAGGGTCATAACCAATCATG+TGG | + | chr5.3:6147854-6147873 | None:intergenic | 45.0% | |
| GAAGCAAGCTAAGGATCTAG+TGG | - | chr5.3:6148309-6148328 | MS.gene09727:CDS | 45.0% | |
| GCACGGGTGAATTACATCTA+CGG | - | chr5.3:6148096-6148115 | MS.gene09727:intron | 45.0% | |
| GGTGCTAAAGAAGCAAGCTA+AGG | - | chr5.3:6148300-6148319 | MS.gene09727:CDS | 45.0% | |
| TATAATCAGGACTCCGTTGG+TGG | - | chr5.3:6148384-6148403 | MS.gene09727:intron | 45.0% | |
| TCACCGATAGCTAAGTGGAT+GGG | - | chr5.3:6147997-6148016 | MS.gene09727:CDS | 45.0% | |
| TGCTTCAACAGTTCATCTCC+AGG | - | chr5.3:6148177-6148196 | MS.gene09727:CDS | 45.0% | |
| TTCCTAACAGAACACACCAG+CGG | + | chr5.3:6148688-6148707 | None:intergenic | 45.0% | |
| TTCGATCACCGATAGCTAAG+TGG | - | chr5.3:6147992-6148011 | MS.gene09727:CDS | 45.0% | |
| ! | AAGGTATCCCCATTTGGACT+TGG | + | chr5.3:6147636-6147655 | None:intergenic | 45.0% |
| ! | GCCCAAGATTTGGAGTCATT+GGG | + | chr5.3:6148483-6148502 | None:intergenic | 45.0% |
| ! | TGCCCAAGATTTGGAGTCAT+TGG | + | chr5.3:6148484-6148503 | None:intergenic | 45.0% |
| !! | ATAAGTCCACCTGTTTCTGC+AGG | - | chr5.3:6148427-6148446 | MS.gene09727:intron | 45.0% |
| !!! | ACCGATGGCTCGGTTATTTT+AGG | + | chr5.3:6148283-6148302 | None:intergenic | 45.0% |
| !!! | TGGACAACCTTTTGAAGCAC+GGG | - | chr5.3:6148080-6148099 | MS.gene09727:intron | 45.0% |
| ACCCACAATGTTTGCCCGTA+TGG | + | chr5.3:6148644-6148663 | None:intergenic | 50.0% | |
| ACGGGCAAACATTGTGGGTT+CGG | - | chr5.3:6148645-6148664 | MS.gene09727:CDS | 50.0% | |
| ACTCTACCTGCAGAAACAGG+TGG | + | chr5.3:6148436-6148455 | None:intergenic | 50.0% | |
| CAAAGCCATCGATCCACCAA+CGG | + | chr5.3:6148400-6148419 | None:intergenic | 50.0% | |
| CATGCTCCTTGCCCAAGATT+TGG | + | chr5.3:6148493-6148512 | None:intergenic | 50.0% | |
| CTGAATAGGAGGCAAGTCCA+CGG | + | chr5.3:6148059-6148078 | None:intergenic | 50.0% | |
| GCAGGTAGAGTTGTACTCGT+TGG | - | chr5.3:6148445-6148464 | MS.gene09727:intron | 50.0% | |
| GGACTTGCCTCCTATTCAGA+TGG | - | chr5.3:6148060-6148079 | MS.gene09727:intron | 50.0% | |
| GTTGGTCATTGTGCTTTCCG+TGG | - | chr5.3:6148039-6148058 | MS.gene09727:intron | 50.0% | |
| TCCTAACAGAACACACCAGC+GGG | + | chr5.3:6148687-6148706 | None:intergenic | 50.0% | |
| TGTGGGTTCGGCTTTGCAAT+GGG | - | chr5.3:6148657-6148676 | MS.gene09727:CDS | 50.0% | |
| TTGTGCTCTTGAGGATGCAG+TGG | - | chr5.3:6148513-6148532 | MS.gene09727:intron | 50.0% | |
| TTGTGGGTTCGGCTTTGCAA+TGG | - | chr5.3:6148656-6148675 | MS.gene09727:CDS | 50.0% | |
| ! | AGGAGCATGTTGTGCTCTTG+AGG | - | chr5.3:6148504-6148523 | MS.gene09727:intron | 50.0% |
| ! | AGTAAGGCTTGGTCCATTGC+TGG | - | chr5.3:6148729-6148748 | MS.gene09727:CDS | 50.0% |
| ! | ATTTGGAGTCATTGGGTGCC+AGG | + | chr5.3:6148476-6148495 | None:intergenic | 50.0% |
| ! | GCTTGCTTCTTTAGCACCGA+TGG | + | chr5.3:6148298-6148317 | None:intergenic | 50.0% |
| ! | GGACCAAGCCTTACTAGCTT+AGG | + | chr5.3:6148724-6148743 | None:intergenic | 50.0% |
| !! | CTTCTTTAGCACCGATGGCT+CGG | + | chr5.3:6148293-6148312 | None:intergenic | 50.0% |
| !!! | AGTGGAGAGGAGGGTACTTT+TGG | - | chr5.3:6147545-6147564 | MS.gene09727:CDS | 50.0% |
| ACCCGCTGGTGTGTTCTGTT+AGG | - | chr5.3:6148683-6148702 | MS.gene09727:CDS | 55.0% | |
| TACGGAAAAGGGTTACGCGC+AGG | - | chr5.3:6148114-6148133 | MS.gene09727:intron | 55.0% | |
| TTGTACTCGTTGGAGACGCC+TGG | - | chr5.3:6148455-6148474 | MS.gene09727:intron | 55.0% | |
| TTTGCAATGGGACAACCCGC+TGG | - | chr5.3:6148669-6148688 | MS.gene09727:CDS | 55.0% | |
| ! | CGTTGGTGGATCGATGGCTT+TGG | - | chr5.3:6148398-6148417 | MS.gene09727:intron | 55.0% |
| TCTCCACTGCACGCACCTCT+TGG | + | chr5.3:6147533-6147552 | None:intergenic | 60.0% | |
| ! | AAGAGGTGCGTGCAGTGGAG+AGG | - | chr5.3:6147532-6147551 | MS.gene09727:CDS | 60.0% |
| ! | GGACTCCGTTGGTGGATCGA+TGG | - | chr5.3:6148392-6148411 | MS.gene09727:intron | 60.0% |
| AGGTGCGTGCAGTGGAGAGG+AGG | - | chr5.3:6147535-6147554 | MS.gene09727:CDS | 65.0% | |
| GGTGCGTGCAGTGGAGAGGA+GGG | - | chr5.3:6147536-6147555 | MS.gene09727:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6147530 | 6148789 | 6147530 | ID=MS.gene09727 |
| chr5.3 | mRNA | 6147530 | 6148789 | 6147530 | ID=MS.gene09727.t1;Parent=MS.gene09727 |
| chr5.3 | exon | 6148627 | 6148789 | 6148627 | ID=MS.gene09727.t1.exon1;Parent=MS.gene09727.t1 |
| chr5.3 | CDS | 6148627 | 6148789 | 6148627 | ID=cds.MS.gene09727.t1;Parent=MS.gene09727.t1 |
| chr5.3 | exon | 6148121 | 6148349 | 6148121 | ID=MS.gene09727.t1.exon2;Parent=MS.gene09727.t1 |
| chr5.3 | CDS | 6148121 | 6148349 | 6148121 | ID=cds.MS.gene09727.t1;Parent=MS.gene09727.t1 |
| chr5.3 | exon | 6147530 | 6148041 | 6147530 | ID=MS.gene09727.t1.exon3;Parent=MS.gene09727.t1 |
| chr5.3 | CDS | 6147530 | 6148041 | 6147530 | ID=cds.MS.gene09727.t1;Parent=MS.gene09727.t1 |
| Gene Sequence |
| Protein sequence |
