Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20315.t1 | XP_003611545.1 | 92.3 | 415 | 29 | 2 | 1 | 414 | 1 | 413 | 1.80E-209 | 738.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20315.t1 | Q9SY57 | 45.7 | 411 | 200 | 5 | 3 | 405 | 4 | 399 | 9.0e-93 | 342.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20315.t1 | G7K2F2 | 92.3 | 415 | 29 | 2 | 1 | 414 | 1 | 413 | 1.3e-209 | 738.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene20315 | 0.807308 | 5.39E-50 | -1.69E-46 |
| MS.gene049150 | MS.gene20315 | 0.866455 | 2.82E-65 | -1.69E-46 |
| MS.gene049151 | MS.gene20315 | 0.824444 | 8.04E-54 | -1.69E-46 |
| MS.gene049153 | MS.gene20315 | 0.859644 | 3.59E-63 | -1.69E-46 |
| MS.gene049260 | MS.gene20315 | 0.812476 | 4.16E-51 | -1.69E-46 |
| MS.gene04933 | MS.gene20315 | 0.801947 | 7.08E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene20315 | 0.811735 | 6.03E-51 | -1.69E-46 |
| MS.gene049356 | MS.gene20315 | 0.858177 | 9.85E-63 | -1.69E-46 |
| MS.gene049378 | MS.gene20315 | 0.813694 | 2.25E-51 | -1.69E-46 |
| MS.gene049443 | MS.gene20315 | 0.801311 | 9.56E-49 | -1.69E-46 |
| MS.gene049662 | MS.gene20315 | 0.804659 | 1.94E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene20315 | 0.848776 | 4.94E-60 | -1.69E-46 |
| MS.gene049910 | MS.gene20315 | 0.835015 | 2.15E-56 | -1.69E-46 |
| MS.gene049915 | MS.gene20315 | 0.808953 | 2.40E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene20315 | 0.842708 | 2.19E-58 | -1.69E-46 |
| MS.gene050168 | MS.gene20315 | 0.805384 | 1.37E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene20315 | 0.829564 | 4.80E-55 | -1.69E-46 |
| MS.gene050227 | MS.gene20315 | 0.819406 | 1.18E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene20315 | 0.813893 | 2.03E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene20315 | 0.866501 | 2.72E-65 | -1.69E-46 |
| MS.gene050397 | MS.gene20315 | 0.802099 | 6.59E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene20315 | -0.819878 | 9.22E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene20315 | 0.814157 | 1.78E-51 | -1.69E-46 |
| MS.gene050529 | MS.gene20315 | 0.823918 | 1.07E-53 | -1.69E-46 |
| MS.gene050623 | MS.gene20315 | -0.808563 | 2.91E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene20315 | 0.820013 | 8.58E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene20315 | 0.853411 | 2.43E-61 | -1.69E-46 |
| MS.gene050820 | MS.gene20315 | 0.806256 | 8.99E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene20315 | 0.837624 | 4.66E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene20315 | 0.816209 | 6.22E-52 | -1.69E-46 |
| MS.gene05119 | MS.gene20315 | -0.802967 | 4.36E-49 | -1.69E-46 |
| MS.gene051360 | MS.gene20315 | 0.824491 | 7.84E-54 | -1.69E-46 |
| MS.gene051525 | MS.gene20315 | 0.816875 | 4.41E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene20315 | 0.878086 | 3.73E-69 | -1.69E-46 |
| MS.gene051983 | MS.gene20315 | 0.858183 | 9.81E-63 | -1.69E-46 |
| MS.gene052011 | MS.gene20315 | -0.847944 | 8.39E-60 | -1.69E-46 |
| MS.gene052077 | MS.gene20315 | 0.860741 | 1.67E-63 | -1.69E-46 |
| MS.gene052178 | MS.gene20315 | 0.803402 | 3.55E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene20315 | 0.903323 | 3.91E-79 | -1.69E-46 |
| MS.gene052253 | MS.gene20315 | 0.870801 | 1.11E-66 | -1.69E-46 |
| MS.gene052255 | MS.gene20315 | 0.832321 | 1.01E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene20315 | 0.816279 | 6.00E-52 | -1.69E-46 |
| MS.gene05233 | MS.gene20315 | -0.802583 | 5.24E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene20315 | 0.877978 | 4.07E-69 | -1.69E-46 |
| MS.gene052414 | MS.gene20315 | 0.807655 | 4.55E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene20315 | 0.822358 | 2.47E-53 | -1.69E-46 |
| MS.gene053073 | MS.gene20315 | 0.804322 | 2.28E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene20315 | 0.847646 | 1.01E-59 | -1.69E-46 |
| MS.gene053209 | MS.gene20315 | 0.818098 | 2.34E-52 | -1.69E-46 |
| MS.gene053494 | MS.gene20315 | 0.822336 | 2.50E-53 | -1.69E-46 |
| MS.gene053557 | MS.gene20315 | 0.832541 | 8.91E-56 | -1.69E-46 |
| MS.gene053559 | MS.gene20315 | 0.82061 | 6.26E-53 | -1.69E-46 |
| MS.gene053577 | MS.gene20315 | 0.83207 | 1.17E-55 | -1.69E-46 |
| MS.gene053758 | MS.gene20315 | 0.803568 | 3.28E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene20315 | 0.874265 | 7.73E-68 | -1.69E-46 |
| MS.gene053928 | MS.gene20315 | 0.818875 | 1.56E-52 | -1.69E-46 |
| MS.gene054010 | MS.gene20315 | 0.814274 | 1.68E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene20315 | 0.825682 | 4.10E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene20315 | 0.820128 | 8.08E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene20315 | 0.812497 | 4.12E-51 | -1.69E-46 |
| MS.gene054123 | MS.gene20315 | 0.839075 | 1.97E-57 | -1.69E-46 |
| MS.gene054183 | MS.gene20315 | 0.863095 | 3.18E-64 | -1.69E-46 |
| MS.gene054349 | MS.gene20315 | 0.862128 | 6.31E-64 | -1.69E-46 |
| MS.gene054473 | MS.gene20315 | 0.819022 | 1.44E-52 | -1.69E-46 |
| MS.gene054542 | MS.gene20315 | 0.821546 | 3.81E-53 | -1.69E-46 |
| MS.gene054556 | MS.gene20315 | 0.825301 | 5.05E-54 | -1.69E-46 |
| MS.gene054719 | MS.gene20315 | 0.802671 | 5.02E-49 | -1.69E-46 |
| MS.gene054918 | MS.gene20315 | 0.818001 | 2.46E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene20315 | 0.870212 | 1.73E-66 | -1.69E-46 |
| MS.gene054983 | MS.gene20315 | 0.830942 | 2.21E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene20315 | 0.86131 | 1.12E-63 | -1.69E-46 |
| MS.gene055277 | MS.gene20315 | 0.803561 | 3.29E-49 | -1.69E-46 |
| MS.gene055369 | MS.gene20315 | 0.80041 | 1.46E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene20315 | 0.838884 | 2.21E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene20315 | 0.807892 | 4.05E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene20315 | 0.849324 | 3.48E-60 | -1.69E-46 |
| MS.gene055604 | MS.gene20315 | 0.845299 | 4.43E-59 | -1.69E-46 |
| MS.gene055666 | MS.gene20315 | 0.820602 | 6.29E-53 | -1.69E-46 |
| MS.gene055859 | MS.gene20315 | 0.82135 | 4.23E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene20315 | 0.832496 | 9.15E-56 | -1.69E-46 |
| MS.gene056447 | MS.gene20315 | 0.832371 | 9.82E-56 | -1.69E-46 |
| MS.gene056511 | MS.gene20315 | 0.823255 | 1.53E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene20315 | 0.829841 | 4.11E-55 | -1.69E-46 |
| MS.gene056659 | MS.gene20315 | 0.87052 | 1.37E-66 | -1.69E-46 |
| MS.gene056723 | MS.gene20315 | 0.845212 | 4.67E-59 | -1.69E-46 |
| MS.gene056899 | MS.gene20315 | -0.837026 | 6.63E-57 | -1.69E-46 |
| MS.gene056902 | MS.gene20315 | 0.819831 | 9.45E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene20315 | 0.859444 | 4.12E-63 | -1.69E-46 |
| MS.gene05702 | MS.gene20315 | 0.828592 | 8.25E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene20315 | 0.821168 | 4.66E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene20315 | 0.810015 | 1.42E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene20315 | 0.801899 | 7.24E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene20315 | 0.844666 | 6.56E-59 | -1.69E-46 |
| MS.gene057448 | MS.gene20315 | 0.853065 | 3.06E-61 | -1.69E-46 |
| MS.gene057449 | MS.gene20315 | 0.834657 | 2.64E-56 | -1.69E-46 |
| MS.gene057450 | MS.gene20315 | 0.851564 | 8.17E-61 | -1.69E-46 |
| MS.gene057469 | MS.gene20315 | 0.852275 | 5.14E-61 | -1.69E-46 |
| MS.gene057476 | MS.gene20315 | 0.803471 | 3.43E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene20315 | 0.838071 | 3.57E-57 | -1.69E-46 |
| MS.gene057750 | MS.gene20315 | 0.807509 | 4.88E-50 | -1.69E-46 |
| MS.gene057796 | MS.gene20315 | 0.81752 | 3.16E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene20315 | 0.837101 | 6.34E-57 | -1.69E-46 |
| MS.gene057825 | MS.gene20315 | 0.816219 | 6.19E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene20315 | 0.848641 | 5.38E-60 | -1.69E-46 |
| MS.gene058007 | MS.gene20315 | 0.813593 | 2.37E-51 | -1.69E-46 |
| MS.gene058082 | MS.gene20315 | 0.81665 | 4.96E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene20315 | 0.854074 | 1.57E-61 | -1.69E-46 |
| MS.gene058142 | MS.gene20315 | 0.862458 | 5.00E-64 | -1.69E-46 |
| MS.gene058218 | MS.gene20315 | -0.802082 | 6.64E-49 | -1.69E-46 |
| MS.gene058365 | MS.gene20315 | 0.816813 | 4.56E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene20315 | 0.80423 | 2.39E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene20315 | 0.816074 | 6.67E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene20315 | 0.869336 | 3.34E-66 | -1.69E-46 |
| MS.gene058749 | MS.gene20315 | 0.819052 | 1.42E-52 | -1.69E-46 |
| MS.gene058845 | MS.gene20315 | 0.810743 | 9.90E-51 | -1.69E-46 |
| MS.gene05886 | MS.gene20315 | 0.850202 | 1.98E-60 | -1.69E-46 |
| MS.gene058928 | MS.gene20315 | 0.852158 | 5.55E-61 | -1.69E-46 |
| MS.gene058948 | MS.gene20315 | 0.801974 | 6.99E-49 | -1.69E-46 |
| MS.gene058949 | MS.gene20315 | 0.863108 | 3.15E-64 | -1.69E-46 |
| MS.gene05907 | MS.gene20315 | 0.862882 | 3.70E-64 | -1.69E-46 |
| MS.gene059082 | MS.gene20315 | 0.807459 | 5.00E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene20315 | 0.883661 | 3.71E-71 | -1.69E-46 |
| MS.gene059294 | MS.gene20315 | 0.829432 | 5.17E-55 | -1.69E-46 |
| MS.gene059366 | MS.gene20315 | 0.848774 | 4.95E-60 | -1.69E-46 |
| MS.gene059420 | MS.gene20315 | 0.873884 | 1.04E-67 | -1.69E-46 |
| MS.gene059462 | MS.gene20315 | 0.83623 | 1.06E-56 | -1.69E-46 |
| MS.gene059597 | MS.gene20315 | 0.859568 | 3.78E-63 | -1.69E-46 |
| MS.gene05959 | MS.gene20315 | 0.807813 | 4.21E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene20315 | 0.828407 | 9.15E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene20315 | 0.852473 | 4.51E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene20315 | 0.852091 | 5.79E-61 | -1.69E-46 |
| MS.gene059695 | MS.gene20315 | 0.849356 | 3.41E-60 | -1.69E-46 |
| MS.gene059807 | MS.gene20315 | 0.838712 | 2.44E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene20315 | 0.887139 | 1.85E-72 | -1.69E-46 |
| MS.gene059876 | MS.gene20315 | 0.818748 | 1.67E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene20315 | 0.833025 | 6.76E-56 | -1.69E-46 |
| MS.gene059899 | MS.gene20315 | 0.805654 | 1.20E-49 | -1.69E-46 |
| MS.gene060247 | MS.gene20315 | 0.823166 | 1.60E-53 | -1.69E-46 |
| MS.gene060407 | MS.gene20315 | 0.80903 | 2.31E-50 | -1.69E-46 |
| MS.gene060495 | MS.gene20315 | 0.851985 | 6.21E-61 | -1.69E-46 |
| MS.gene061002 | MS.gene20315 | 0.805767 | 1.14E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene20315 | 0.884698 | 1.54E-71 | -1.69E-46 |
| MS.gene061051 | MS.gene20315 | -0.838084 | 3.55E-57 | -1.69E-46 |
| MS.gene061114 | MS.gene20315 | -0.81163 | 6.36E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene20315 | 0.828645 | 8.01E-55 | -1.69E-46 |
| MS.gene061159 | MS.gene20315 | 0.844874 | 5.77E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20315.t1 | MTR_5g015150 | 92.289 | 415 | 29 | 2 | 1 | 414 | 1 | 413 | 0.0 | 749 |
| MS.gene20315.t1 | MTR_3g103320 | 44.131 | 426 | 197 | 7 | 1 | 414 | 1 | 397 | 9.01e-118 | 349 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20315.t1 | AT1G10150 | 46.135 | 414 | 194 | 6 | 3 | 405 | 4 | 399 | 9.81e-122 | 360 |
| MS.gene20315.t1 | AT1G59510 | 42.965 | 398 | 177 | 9 | 14 | 406 | 15 | 367 | 5.69e-107 | 321 |
| MS.gene20315.t1 | AT3G49790 | 41.832 | 404 | 184 | 5 | 10 | 413 | 12 | 364 | 9.23e-99 | 299 |
Find 75 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTGTTCTGATTTGGATTC+TGG | 0.226641 | 5.1:-7798595 | MS.gene20315:CDS |
| TAACCCTAGTCTCATGTTTC+GGG | 0.244852 | 5.1:+7798419 | None:intergenic |
| GCAATTTGATTCAAACTATT+AGG | 0.252909 | 5.1:+7798898 | None:intergenic |
| CTAACCCTAGTCTCATGTTT+CGG | 0.262134 | 5.1:+7798418 | None:intergenic |
| GATAGTTCAGGTTCAGGTTC+AGG | 0.314416 | 5.1:-7798310 | MS.gene20315:CDS |
| TGAGACAGTTAGATCTTTCA+TGG | 0.315546 | 5.1:-7798113 | MS.gene20315:CDS |
| ATTGTTTACACCGGCTGGTT+CGG | 0.330132 | 5.1:-7798728 | MS.gene20315:CDS |
| TTGTTTACACCGGCTGGTTC+GGG | 0.342746 | 5.1:-7798727 | MS.gene20315:CDS |
| CCCGATTGAAACTAAGATTA+AGG | 0.352373 | 5.1:+7799104 | None:intergenic |
| AGAATCGGGTTGTTCTGATT+TGG | 0.353612 | 5.1:-7798602 | MS.gene20315:CDS |
| TTTGCTAGGAATATTGTTCT+TGG | 0.353935 | 5.1:-7798679 | MS.gene20315:CDS |
| ACCTTAATCTTAGTTTCAAT+CGG | 0.367438 | 5.1:-7799105 | MS.gene20315:CDS |
| TGGATTCTGGTGTGCCGAAA+TGG | 0.379309 | 5.1:-7798582 | MS.gene20315:CDS |
| CAAACAACATCAACCCATTT+CGG | 0.385507 | 5.1:+7798568 | None:intergenic |
| TTGCTAGGAATATTGTTCTT+GGG | 0.385636 | 5.1:-7798678 | MS.gene20315:CDS |
| CATTGGCTATTCCTAGTAAT+AGG | 0.391171 | 5.1:-7798171 | MS.gene20315:CDS |
| CCGAATGATAGTTCAGGTTC+AGG | 0.392010 | 5.1:-7798316 | MS.gene20315:CDS |
| TGCACATAATGGATTGCAAT+TGG | 0.408920 | 5.1:-7797958 | MS.gene20315:CDS |
| GGATTTGCTTCTGTTGTTGT+TGG | 0.421636 | 5.1:-7798706 | MS.gene20315:CDS |
| AATGGATTGCAATTGGGCTT+TGG | 0.425168 | 5.1:-7797951 | MS.gene20315:CDS |
| AAGAGATGTTTGTGATAAAG+AGG | 0.428216 | 5.1:-7798227 | MS.gene20315:CDS |
| TTTCTCTTTCGAGCTATGAA+TGG | 0.431945 | 5.1:+7799045 | None:intergenic |
| CTTGTCAGCAACATTTGAAT+CGG | 0.434555 | 5.1:+7798762 | None:intergenic |
| ATAAGTGTGGGGAGCTGATT+GGG | 0.438595 | 5.1:-7798540 | MS.gene20315:CDS |
| GTTTGTGATAAAGAGGATAA+AGG | 0.441922 | 5.1:-7798220 | MS.gene20315:CDS |
| TCGAACCCGAATGATAGTTC+AGG | 0.449003 | 5.1:-7798322 | MS.gene20315:CDS |
| CCTGAACCTGAACTATCATT+CGG | 0.449100 | 5.1:+7798316 | None:intergenic |
| GGATTCGAGAAGTGGAGAAT+CGG | 0.454786 | 5.1:-7798617 | MS.gene20315:CDS |
| ATGTCCGTAAAAGAAAGAAA+TGG | 0.457589 | 5.1:-7799128 | MS.gene20315:CDS |
| GCACATAATGGATTGCAATT+GGG | 0.461406 | 5.1:-7797957 | MS.gene20315:CDS |
| CGCGTTGTGCCTGCACATAA+TGG | 0.463386 | 5.1:-7797969 | MS.gene20315:CDS |
| CCTTAATCTTAGTTTCAATC+GGG | 0.466170 | 5.1:-7799104 | MS.gene20315:CDS |
| CTGAACCTGAACTATCATTC+GGG | 0.470073 | 5.1:+7798317 | None:intergenic |
| AGACTTGTTCTTGATGTGAC+TGG | 0.473332 | 5.1:-7798148 | MS.gene20315:CDS |
| GACTAGGGTTAGAGAAATGT+TGG | 0.481635 | 5.1:-7798407 | MS.gene20315:CDS |
| GATAAATTGTTTACACCGGC+TGG | 0.484801 | 5.1:-7798733 | MS.gene20315:CDS |
| GGATTCTGGTGTGCCGAAAT+GGG | 0.490037 | 5.1:-7798581 | MS.gene20315:CDS |
| GATAAGTGTGGGGAGCTGAT+TGG | 0.493181 | 5.1:-7798541 | MS.gene20315:CDS |
| AGAACAAGTCTCCTATTACT+AGG | 0.493344 | 5.1:+7798160 | None:intergenic |
| CGATTCAAATGTTGCTGACA+AGG | 0.496180 | 5.1:-7798761 | MS.gene20315:CDS |
| TAAAGAGGATAAAGGTGGTT+GGG | 0.499685 | 5.1:-7798212 | MS.gene20315:CDS |
| GATTCGAGAAGTGGAGAATC+GGG | 0.500290 | 5.1:-7798616 | MS.gene20315:CDS |
| CTAACCCGAAACATGAGACT+AGG | 0.506984 | 5.1:-7798423 | MS.gene20315:CDS |
| CTAGTAACCACCACTTAAGC+AGG | 0.514335 | 5.1:+7797926 | None:intergenic |
| GTGACTGTTGGAATTGTGAA+TGG | 0.524223 | 5.1:-7798820 | MS.gene20315:CDS |
| ATGTTGGTTGATGTTTGTAA+TGG | 0.527695 | 5.1:-7798391 | MS.gene20315:CDS |
| TCAATTGTTTGTTAGTACAT+TGG | 0.532506 | 5.1:-7798509 | MS.gene20315:CDS |
| TTGTTTGTAGTGATAAGTGT+GGG | 0.535485 | 5.1:-7798552 | MS.gene20315:CDS |
| TGGTTATCAATCCATGAGCC+AGG | 0.539703 | 5.1:-7798800 | MS.gene20315:CDS |
| GACTTGTTCTTGATGTGACT+GGG | 0.540843 | 5.1:-7798147 | MS.gene20315:CDS |
| GGAGGGTTGGATTCGAGAAG+TGG | 0.544923 | 5.1:-7798625 | MS.gene20315:CDS |
| TCACAATGTTCACGAGGAAG+TGG | 0.550752 | 5.1:-7798047 | MS.gene20315:CDS |
| AATTGCAATCCATTATGTGC+AGG | 0.556385 | 5.1:+7797960 | None:intergenic |
| ATAAAGAGGATAAAGGTGGT+TGG | 0.557867 | 5.1:-7798213 | MS.gene20315:CDS |
| TGGTAAATGCAGTGGAGGGT+TGG | 0.560656 | 5.1:-7798638 | MS.gene20315:CDS |
| GTTTGATAAATTGTTTACAC+CGG | 0.572672 | 5.1:-7798737 | MS.gene20315:CDS |
| CAGCAAGGTTTCATCCACAT+TGG | 0.581109 | 5.1:-7798188 | MS.gene20315:CDS |
| GTTGTTTGTAGTGATAAGTG+TGG | 0.581476 | 5.1:-7798553 | MS.gene20315:CDS |
| GTGACACGTGCTGTGACTGT+TGG | 0.593196 | 5.1:-7798832 | MS.gene20315:CDS |
| GAAGCAAATCCCGAACCAGC+CGG | 0.594026 | 5.1:+7798718 | None:intergenic |
| TAAAGGTGGTTGGGTCAGCA+AGG | 0.596356 | 5.1:-7798203 | MS.gene20315:CDS |
| AATTGCGAAATTATCGCGCT+CGG | 0.597238 | 5.1:-7798881 | MS.gene20315:CDS |
| TAACCCGAAACATGAGACTA+GGG | 0.605610 | 5.1:-7798422 | MS.gene20315:CDS |
| TCAGAATCTGCAGATACCAT+CGG | 0.615540 | 5.1:-7798973 | MS.gene20315:CDS |
| TTGTTCTTGATGTGACTGGG+AGG | 0.633345 | 5.1:-7798144 | MS.gene20315:CDS |
| TGTTCTTGATGTGACTGGGA+GGG | 0.644691 | 5.1:-7798143 | MS.gene20315:CDS |
| TCTTGTTAAAACGTCTCATG+AGG | 0.649097 | 5.1:-7798356 | MS.gene20315:CDS |
| GCTGGTGGTAAATGCAGTGG+AGG | 0.658148 | 5.1:-7798643 | MS.gene20315:CDS |
| ATTACTAGGAATAGCCAATG+TGG | 0.658823 | 5.1:+7798174 | None:intergenic |
| GATGCTGGTGGTAAATGCAG+TGG | 0.670323 | 5.1:-7798646 | MS.gene20315:CDS |
| TGTGATAAAGAGGATAAAGG+TGG | 0.671559 | 5.1:-7798217 | MS.gene20315:CDS |
| ATGTGCTCACAATGTTCACG+AGG | 0.700633 | 5.1:-7798053 | MS.gene20315:CDS |
| CTGGTGGTAAATGCAGTGGA+GGG | 0.702499 | 5.1:-7798642 | MS.gene20315:CDS |
| TGAATGGTGCGTGATAAGCA+CGG | 0.702699 | 5.1:+7799061 | None:intergenic |
| TGTTTGTAGTGATAAGTGTG+GGG | 0.727200 | 5.1:-7798551 | MS.gene20315:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACCTTAATCTTAGTTTCAAT+CGG | - | chr5.1:7797997-7798016 | MS.gene20315:CDS | 25.0% |
| ! | GCAATTTGATTCAAACTATT+AGG | + | chr5.1:7798207-7798226 | None:intergenic | 25.0% |
| ! | GTTTGATAAATTGTTTACAC+CGG | - | chr5.1:7798365-7798384 | MS.gene20315:CDS | 25.0% |
| !! | ACTTATGATGAATTCTTTTC+TGG | - | chr5.1:7798651-7798670 | MS.gene20315:CDS | 25.0% |
| !! | TCAATTGTTTGTTAGTACAT+TGG | - | chr5.1:7798593-7798612 | MS.gene20315:CDS | 25.0% |
| AAGAGATGTTTGTGATAAAG+AGG | - | chr5.1:7798875-7798894 | MS.gene20315:CDS | 30.0% | |
| ATGTCCGTAAAAGAAAGAAA+TGG | - | chr5.1:7797974-7797993 | MS.gene20315:CDS | 30.0% | |
| CCTTAATCTTAGTTTCAATC+GGG | - | chr5.1:7797998-7798017 | MS.gene20315:CDS | 30.0% | |
| GTTTGTGATAAAGAGGATAA+AGG | - | chr5.1:7798882-7798901 | MS.gene20315:CDS | 30.0% | |
| TTGCTAGGAATATTGTTCTT+GGG | - | chr5.1:7798424-7798443 | MS.gene20315:CDS | 30.0% | |
| TTGTTTGTAGTGATAAGTGT+GGG | - | chr5.1:7798550-7798569 | MS.gene20315:CDS | 30.0% | |
| TTTGCTAGGAATATTGTTCT+TGG | - | chr5.1:7798423-7798442 | MS.gene20315:CDS | 30.0% | |
| ! | AGGTCCATTTCTTTCTTTTA+CGG | + | chr5.1:7797981-7798000 | None:intergenic | 30.0% |
| ! | ATGTTGGTTGATGTTTGTAA+TGG | - | chr5.1:7798711-7798730 | MS.gene20315:CDS | 30.0% |
| !!! | TTTATTTTGCAAACGTTGTG+TGG | - | chr5.1:7799014-7799033 | MS.gene20315:CDS | 30.0% |
| AATTGCAATCCATTATGTGC+AGG | + | chr5.1:7799145-7799164 | None:intergenic | 35.0% | |
| AGAACAAGTCTCCTATTACT+AGG | + | chr5.1:7798945-7798964 | None:intergenic | 35.0% | |
| ATAAAGAGGATAAAGGTGGT+TGG | - | chr5.1:7798889-7798908 | MS.gene20315:CDS | 35.0% | |
| ATTACTAGGAATAGCCAATG+TGG | + | chr5.1:7798931-7798950 | None:intergenic | 35.0% | |
| CAAACAACATCAACCCATTT+CGG | + | chr5.1:7798537-7798556 | None:intergenic | 35.0% | |
| CCCGATTGAAACTAAGATTA+AGG | + | chr5.1:7798001-7798020 | None:intergenic | 35.0% | |
| CTTGTCAGCAACATTTGAAT+CGG | + | chr5.1:7798343-7798362 | None:intergenic | 35.0% | |
| GTTGTTTGTAGTGATAAGTG+TGG | - | chr5.1:7798549-7798568 | MS.gene20315:CDS | 35.0% | |
| TAAAGAGGATAAAGGTGGTT+GGG | - | chr5.1:7798890-7798909 | MS.gene20315:CDS | 35.0% | |
| TCTTGTTAAAACGTCTCATG+AGG | - | chr5.1:7798746-7798765 | MS.gene20315:CDS | 35.0% | |
| TGAGACAGTTAGATCTTTCA+TGG | - | chr5.1:7798989-7799008 | MS.gene20315:CDS | 35.0% | |
| TGTGATAAAGAGGATAAAGG+TGG | - | chr5.1:7798885-7798904 | MS.gene20315:CDS | 35.0% | |
| TGTTTGTAGTGATAAGTGTG+GGG | - | chr5.1:7798551-7798570 | MS.gene20315:CDS | 35.0% | |
| TTTCTCTTTCGAGCTATGAA+TGG | + | chr5.1:7798060-7798079 | None:intergenic | 35.0% | |
| ! | ATCTTTTGTGACAATACCGA+TGG | + | chr5.1:7798148-7798167 | None:intergenic | 35.0% |
| ! | CATTGGCTATTCCTAGTAAT+AGG | - | chr5.1:7798931-7798950 | MS.gene20315:CDS | 35.0% |
| !! | GCACATAATGGATTGCAATT+GGG | - | chr5.1:7799145-7799164 | MS.gene20315:CDS | 35.0% |
| !! | TGCACATAATGGATTGCAAT+TGG | - | chr5.1:7799144-7799163 | MS.gene20315:CDS | 35.0% |
| !! | TTGTTGTTGGAAGTTTTGCT+AGG | - | chr5.1:7798409-7798428 | MS.gene20315:CDS | 35.0% |
| !!! | TCAGGTTCTTATTTTGATGC+TGG | - | chr5.1:7798810-7798829 | MS.gene20315:CDS | 35.0% |
| AATTGCGAAATTATCGCGCT+CGG | - | chr5.1:7798221-7798240 | MS.gene20315:CDS | 40.0% | |
| CCTGAACCTGAACTATCATT+CGG | + | chr5.1:7798789-7798808 | None:intergenic | 40.0% | |
| CGATTCAAATGTTGCTGACA+AGG | - | chr5.1:7798341-7798360 | MS.gene20315:CDS | 40.0% | |
| CTAACCCTAGTCTCATGTTT+CGG | + | chr5.1:7798687-7798706 | None:intergenic | 40.0% | |
| CTGAACCTGAACTATCATTC+GGG | + | chr5.1:7798788-7798807 | None:intergenic | 40.0% | |
| GACTAGGGTTAGAGAAATGT+TGG | - | chr5.1:7798695-7798714 | MS.gene20315:CDS | 40.0% | |
| GATAAATTGTTTACACCGGC+TGG | - | chr5.1:7798369-7798388 | MS.gene20315:CDS | 40.0% | |
| GTGACTGTTGGAATTGTGAA+TGG | - | chr5.1:7798282-7798301 | MS.gene20315:CDS | 40.0% | |
| TAACCCGAAACATGAGACTA+GGG | - | chr5.1:7798680-7798699 | MS.gene20315:CDS | 40.0% | |
| TAACCCTAGTCTCATGTTTC+GGG | + | chr5.1:7798686-7798705 | None:intergenic | 40.0% | |
| TCAGAATCTGCAGATACCAT+CGG | - | chr5.1:7798129-7798148 | MS.gene20315:CDS | 40.0% | |
| ! | AGAATCGGGTTGTTCTGATT+TGG | - | chr5.1:7798500-7798519 | MS.gene20315:CDS | 40.0% |
| ! | AGACTTGTTCTTGATGTGAC+TGG | - | chr5.1:7798954-7798973 | MS.gene20315:CDS | 40.0% |
| ! | GACTTGTTCTTGATGTGACT+GGG | - | chr5.1:7798955-7798974 | MS.gene20315:CDS | 40.0% |
| ! | GGATTTGCTTCTGTTGTTGT+TGG | - | chr5.1:7798396-7798415 | MS.gene20315:CDS | 40.0% |
| !! | AATGGATTGCAATTGGGCTT+TGG | - | chr5.1:7799151-7799170 | MS.gene20315:CDS | 40.0% |
| !! | GGTTGTTCTGATTTGGATTC+TGG | - | chr5.1:7798507-7798526 | MS.gene20315:CDS | 40.0% |
| !!! | CTTGGGTTTTATTCAGATGC+TGG | - | chr5.1:7798441-7798460 | MS.gene20315:CDS | 40.0% |
| ATAAGTGTGGGGAGCTGATT+GGG | - | chr5.1:7798562-7798581 | MS.gene20315:CDS | 45.0% | |
| ATGTGCTCACAATGTTCACG+AGG | - | chr5.1:7799049-7799068 | MS.gene20315:CDS | 45.0% | |
| CAGCAAGGTTTCATCCACAT+TGG | - | chr5.1:7798914-7798933 | MS.gene20315:CDS | 45.0% | |
| CTAACCCGAAACATGAGACT+AGG | - | chr5.1:7798679-7798698 | MS.gene20315:CDS | 45.0% | |
| GATTCGAGAAGTGGAGAATC+GGG | - | chr5.1:7798486-7798505 | MS.gene20315:CDS | 45.0% | |
| GGATTCGAGAAGTGGAGAAT+CGG | - | chr5.1:7798485-7798504 | MS.gene20315:CDS | 45.0% | |
| TCACAATGTTCACGAGGAAG+TGG | - | chr5.1:7799055-7799074 | MS.gene20315:CDS | 45.0% | |
| TCGAACCCGAATGATAGTTC+AGG | - | chr5.1:7798780-7798799 | MS.gene20315:CDS | 45.0% | |
| TGAATGGTGCGTGATAAGCA+CGG | + | chr5.1:7798044-7798063 | None:intergenic | 45.0% | |
| TGGTTATCAATCCATGAGCC+AGG | - | chr5.1:7798302-7798321 | MS.gene20315:CDS | 45.0% | |
| TGTTCTTGATGTGACTGGGA+GGG | - | chr5.1:7798959-7798978 | MS.gene20315:CDS | 45.0% | |
| TTGTTCTTGATGTGACTGGG+AGG | - | chr5.1:7798958-7798977 | MS.gene20315:CDS | 45.0% | |
| ! | ATTGTTTACACCGGCTGGTT+CGG | - | chr5.1:7798374-7798393 | MS.gene20315:CDS | 45.0% |
| ! | ATTTTCATCAGCCTGGCTCA+TGG | + | chr5.1:7798316-7798335 | None:intergenic | 45.0% |
| ! | CCGAATGATAGTTCAGGTTC+AGG | - | chr5.1:7798786-7798805 | MS.gene20315:CDS | 45.0% |
| ! | GATAGTTCAGGTTCAGGTTC+AGG | - | chr5.1:7798792-7798811 | MS.gene20315:CDS | 45.0% |
| !! | CGGATTGATTTTCATCAGCC+TGG | + | chr5.1:7798323-7798342 | None:intergenic | 45.0% |
| !!! | GGAACAGTTTTCGGGTTCTT+TGG | - | chr5.1:7798242-7798261 | MS.gene20315:CDS | 45.0% |
| !!! | GGGTTTTATTCAGATGCTGG+TGG | - | chr5.1:7798444-7798463 | MS.gene20315:CDS | 45.0% |
| CTGGTGGTAAATGCAGTGGA+GGG | - | chr5.1:7798460-7798479 | MS.gene20315:CDS | 50.0% | |
| GATAAGTGTGGGGAGCTGAT+TGG | - | chr5.1:7798561-7798580 | MS.gene20315:CDS | 50.0% | |
| GATGCTGGTGGTAAATGCAG+TGG | - | chr5.1:7798456-7798475 | MS.gene20315:CDS | 50.0% | |
| GGATTCTGGTGTGCCGAAAT+GGG | - | chr5.1:7798521-7798540 | MS.gene20315:CDS | 50.0% | |
| TAAAGGTGGTTGGGTCAGCA+AGG | - | chr5.1:7798899-7798918 | MS.gene20315:CDS | 50.0% | |
| TGGATTCTGGTGTGCCGAAA+TGG | - | chr5.1:7798520-7798539 | MS.gene20315:CDS | 50.0% | |
| TGGTAAATGCAGTGGAGGGT+TGG | - | chr5.1:7798464-7798483 | MS.gene20315:CDS | 50.0% | |
| ! | ATCGCGCTCGGAACAGTTTT+CGG | - | chr5.1:7798233-7798252 | MS.gene20315:CDS | 50.0% |
| ! | TTGTTTACACCGGCTGGTTC+GGG | - | chr5.1:7798375-7798394 | MS.gene20315:CDS | 50.0% |
| CGCGTTGTGCCTGCACATAA+TGG | - | chr5.1:7799133-7799152 | MS.gene20315:CDS | 55.0% | |
| GAAGCAAATCCCGAACCAGC+CGG | + | chr5.1:7798387-7798406 | None:intergenic | 55.0% | |
| GCTGGTGGTAAATGCAGTGG+AGG | - | chr5.1:7798459-7798478 | MS.gene20315:CDS | 55.0% | |
| GGAGGGTTGGATTCGAGAAG+TGG | - | chr5.1:7798477-7798496 | MS.gene20315:CDS | 55.0% | |
| GTGACACGTGCTGTGACTGT+TGG | - | chr5.1:7798270-7798289 | MS.gene20315:CDS | 55.0% | |
| ! | TCGCGCTCGGAACAGTTTTC+GGG | - | chr5.1:7798234-7798253 | MS.gene20315:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 7797940 | 7799184 | 7797940 | ID=MS.gene20315 |
| chr5.1 | mRNA | 7797940 | 7799184 | 7797940 | ID=MS.gene20315.t1;Parent=MS.gene20315 |
| chr5.1 | exon | 7797940 | 7799184 | 7797940 | ID=MS.gene20315.t1.exon1;Parent=MS.gene20315.t1 |
| chr5.1 | CDS | 7797940 | 7799184 | 7797940 | ID=cds.MS.gene20315.t1;Parent=MS.gene20315.t1 |
| Gene Sequence |
| Protein sequence |
