AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52105

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52105.t1 MTR_4g074260 99.614 259 1 0 1 259 1 259 0.0 521
MS.gene52105.t1 MTR_7g027235 93.077 260 17 1 1 259 1 260 6.71e-179 492
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52105.t1 AT3G62870 88.031 259 28 1 1 259 1 256 1.85e-170 471
MS.gene52105.t1 AT2G47610 87.552 241 30 0 19 259 17 257 1.44e-161 448

Find 83 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGTCTTGGCTTGGGATT+TGG 0.209141 4.1:+29666641 None:intergenic
AGGCCATACTTCACATTAAT+AGG 0.264776 4.1:+29667263 None:intergenic
GAGAAGCGTCCAAAGCAATT+TGG 0.271255 4.1:-29668527 MS.gene52105:CDS
CTTTGCCTTGGTCTTGGCTT+GGG 0.278418 4.1:+29666635 None:intergenic
CAGGGTCCTAGAGGCGATTA+AGG 0.309642 4.1:-29666815 MS.gene52105:intron
CCTTTCCTTTGCCTTGGTCT+TGG 0.313711 4.1:+29666629 None:intergenic
CTTCGGCCACTTCACGAATC+TGG 0.319581 4.1:+29668469 None:intergenic
CCTTTGCCTTGGTCTTGGCT+TGG 0.320697 4.1:+29666634 None:intergenic
TTCTTCTTTGAAGCTGCAAC+TGG 0.338108 4.1:+29668674 None:intergenic
TTGCCCTTAACAATACAGTA+TGG 0.340642 4.1:+29667016 None:intergenic
CATTCTCTGGGCAGCCTCCT+TGG 0.347883 4.1:+29666599 None:intergenic
CCTCCAATTCCAAATTGCTT+TGG 0.359017 4.1:+29668518 None:intergenic
AAGCCTATTAATGTGAAGTA+TGG 0.361147 4.1:-29667266 MS.gene52105:CDS
CGTCCAAAGCAATTTGGAAT+TGG 0.369614 4.1:-29668521 MS.gene52105:CDS
TTCTTCTCAGCCTTGTCCTC+AGG 0.377096 4.1:+29667347 None:intergenic
CTCTGCACCTGAACGTTCTT+CGG 0.382996 4.1:+29668452 None:intergenic
GAAGTGGGGTGGTGGAATCA+TGG 0.397393 4.1:-29666667 MS.gene52105:CDS
AATCCCATACTGTATTGTTA+AGG 0.413635 4.1:-29667020 MS.gene52105:CDS
AATAAGCCTTTCCTTTGCCT+TGG 0.418296 4.1:+29666623 None:intergenic
TCTGGCTTCCAGCCTTGTGC+AGG 0.421984 4.1:-29667051 MS.gene52105:CDS
ACAACTCCTAGGTCATTCTC+TGG 0.422899 4.1:+29666586 None:intergenic
TCCATCTTCCTGCACAAGGC+TGG 0.428911 4.1:+29667043 None:intergenic
AAGGGCTCAGGCAGAGGCTG+AGG 0.430646 4.1:-29667309 MS.gene52105:CDS
TACAGGAAGAAGTGGGGTGG+TGG 0.431668 4.1:-29666675 MS.gene52105:CDS
TCCAGCCTTGTGCAGGAAGA+TGG 0.436588 4.1:-29667044 MS.gene52105:CDS
ACCCCATTGAATTAGTTGTC+TGG 0.442276 4.1:-29667069 MS.gene52105:CDS
AGCCAGACAACTAATTCAAT+GGG 0.443985 4.1:+29667067 None:intergenic
TGAGGAGTACAGGAAGAAGT+GGG 0.451890 4.1:-29666682 MS.gene52105:CDS
ATCCCATACTGTATTGTTAA+GGG 0.456742 4.1:-29667019 MS.gene52105:CDS
AAGACAAAATGGAGTTCAGC+AGG 0.470262 4.1:-29666834 MS.gene52105:CDS
ATGAGGAGTACAGGAAGAAG+TGG 0.472066 4.1:-29666683 MS.gene52105:CDS
CGTGAGCAATCACGACAAGC+TGG 0.479843 4.1:+29667098 None:intergenic
CCAAAGCAATTTGGAATTGG+AGG 0.482647 4.1:-29668518 MS.gene52105:CDS
TCAAGATACTTCTGAAGTAC+AGG 0.489642 4.1:-29667370 MS.gene52105:CDS
TTTGAAAAGGGCTCAGGCAG+AGG 0.490294 4.1:-29667315 MS.gene52105:CDS
AAGTGGGGTGGTGGAATCAT+GGG 0.491130 4.1:-29666666 MS.gene52105:CDS
AAGCCAGACAACTAATTCAA+TGG 0.495839 4.1:+29667066 None:intergenic
TGAGGACAAGGCTGAGAAGA+AGG 0.500213 4.1:-29667345 MS.gene52105:CDS
TTAGTGAACTGGTTCAAAGC+TGG 0.502229 4.1:+29668392 None:intergenic
TTGGACGCTTCTCGAATAGC+GGG 0.505390 4.1:+29668537 None:intergenic
GAAGTGGCCGAAGAACGTTC+AGG 0.510705 4.1:-29668459 MS.gene52105:CDS
ATGACAAGTATGAGGAGTAC+AGG 0.517239 4.1:-29666692 MS.gene52105:CDS
GAAGTACAGGCCTGAGGACA+AGG 0.533130 4.1:-29667357 MS.gene52105:CDS
AGGTGCATTGCCGCCGAAGA+GGG 0.544548 4.1:-29668498 MS.gene52105:CDS
ACAACAAACTTACACTTCCT+AGG 0.547646 4.1:+29666987 None:intergenic
TGCCCTTAACAATACAGTAT+GGG 0.553243 4.1:+29667017 None:intergenic
CAAACTTACACTTCCTAGGC+GGG 0.561796 4.1:+29666991 None:intergenic
ATTTGACCAGATTCGTGAAG+TGG 0.563086 4.1:-29668475 MS.gene52105:CDS
AAATTTCAATGACAAGTATG+AGG 0.565347 4.1:-29666700 MS.gene52105:CDS
GTGAACTGGTTCAAAGCTGG+TGG 0.574137 4.1:+29668395 None:intergenic
TTCAGGTGCAGAGGAAGAAG+AGG 0.574820 4.1:-29668442 MS.gene52105:CDS
AGGGCTCAGGCAGAGGCTGA+GGG 0.575749 4.1:-29667308 MS.gene52105:CDS
ATGCCACCTTAATCGCCTCT+AGG 0.580251 4.1:+29666809 None:intergenic
AATCTGGTCAAATCCCTCTT+CGG 0.586903 4.1:+29668485 None:intergenic
GTTAAGGGCAAGGCCCGCCT+AGG 0.589576 4.1:-29667004 MS.gene52105:CDS
GTGAGCAATCACGACAAGCT+GGG 0.590287 4.1:+29667099 None:intergenic
CGAAGAACGTTCAGGTGCAG+AGG 0.592402 4.1:-29668451 MS.gene52105:CDS
TTTGGACGCTTCTCGAATAG+CGG 0.595981 4.1:+29668536 None:intergenic
GAGGTGCATTGCCGCCGAAG+AGG 0.597405 4.1:-29668499 MS.gene52105:CDS
CCAAGCCAAGACCAAGGCAA+AGG 0.598075 4.1:-29666634 MS.gene52105:CDS
GATTTCCATCTTCCTGCACA+AGG 0.600419 4.1:+29667039 None:intergenic
ATACTGTATTGTTAAGGGCA+AGG 0.604440 4.1:-29667014 MS.gene52105:CDS
TGAGGTAGGTAACATGATTG+AGG 0.604638 4.1:+29667243 None:intergenic
ACAAGGCTGAGAAGAAGGAG+AGG 0.612342 4.1:-29667340 MS.gene52105:CDS
AAAGGAAAGGCTTATTGCCA+AGG 0.613232 4.1:-29666616 MS.gene52105:CDS
TGTTACCTACCTCATCGAGC+AGG 0.615302 4.1:-29667234 MS.gene52105:intron
GGCTGCCCAGAGAATGACCT+AGG 0.616298 4.1:-29666592 None:intergenic
AAGTATCTTGAACAAGCTTG+TGG 0.617064 4.1:+29667382 None:intergenic
ACGCTTCTCGAATAGCGGGT+TGG 0.618890 4.1:+29668541 None:intergenic
CCAAGACCAAGGCAAAGGAA+AGG 0.622185 4.1:-29666629 MS.gene52105:CDS
GAGTACAGGAAGAAGTGGGG+TGG 0.625879 4.1:-29666678 MS.gene52105:CDS
GAGGAGTACAGGAAGAAGTG+GGG 0.627370 4.1:-29666681 MS.gene52105:CDS
GGAGTTCAGCAGGGTCCTAG+AGG 0.633517 4.1:-29666824 MS.gene52105:CDS
CTGGTCAAATCCCTCTTCGG+CGG 0.649718 4.1:+29668488 None:intergenic
CAACTCCTAGGTCATTCTCT+GGG 0.652662 4.1:+29666587 None:intergenic
GGAAAGGCTTATTGCCAAGG+AGG 0.665686 4.1:-29666613 MS.gene52105:CDS
CAAATCCCAAGCCAAGACCA+AGG 0.672977 4.1:-29666640 MS.gene52105:CDS
ACAAACTTACACTTCCTAGG+CGG 0.677034 4.1:+29666990 None:intergenic
GAAAGACAACCTGCTCGATG+AGG 0.683651 4.1:+29667225 None:intergenic
GACAACCTGCTCGATGAGGT+AGG 0.683860 4.1:+29667229 None:intergenic
AGACAAAATGGAGTTCAGCA+GGG 0.687168 4.1:-29666833 MS.gene52105:CDS
ACTTCTGAAGTACAGGCCTG+AGG 0.703947 4.1:-29667363 MS.gene52105:CDS
GCCAGACAACTAATTCAATG+GGG 0.748421 4.1:+29667068 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAATTGTAAAATTAATAT+AGG - chr4.1:29667155-29667174 MS.gene52105:intron 10.0%
!! AATTATTGTAATGTTATGTT+TGG - chr4.1:29667065-29667084 MS.gene52105:CDS 15.0%
!! CAGTAAAATAACAAATTAAA+AGG + chr4.1:29668236-29668255 None:intergenic 15.0%
!! GTAAAATTAATATAGGATTT+CGG - chr4.1:29667162-29667181 MS.gene52105:intron 15.0%
!!! TTTTTGTAATATGTTAATTG+TGG - chr4.1:29667133-29667152 MS.gene52105:intron 15.0%
!! AAAACCAACATAAAACATAT+CGG + chr4.1:29668675-29668694 None:intergenic 20.0%
!! AATCAGTATATTGTTATATC+AGG + chr4.1:29667933-29667952 None:intergenic 20.0%
!! ATCAGTATATTGTTATATCA+GGG + chr4.1:29667932-29667951 None:intergenic 20.0%
!! TGTTAAAAATGAAGACAAAA+TGG - chr4.1:29668559-29668578 MS.gene52105:intron 20.0%
!! TTCAGTCATAAATCATATAT+AGG + chr4.1:29668453-29668472 None:intergenic 20.0%
!!! AGTTATTTCAAAAGCTTAAA+TGG + chr4.1:29667618-29667637 None:intergenic 20.0%
!!! ATAAAACATATCGGTTTAAA+TGG + chr4.1:29668666-29668685 None:intergenic 20.0%
!!! GTTATTTCAAAAGCTTAAAT+GGG + chr4.1:29667617-29667636 None:intergenic 20.0%
!!! TAAGCAAATTTTGACTTTTT+AGG + chr4.1:29667489-29667508 None:intergenic 20.0%
!!! TTTTATGTTGGTTTTATTTC+AGG - chr4.1:29668680-29668699 MS.gene52105:CDS 20.0%
! AAATTTCAATGACAAGTATG+AGG - chr4.1:29668704-29668723 MS.gene52105:intron 25.0%
! ACGTTAAACAATTATTCGTT+AGG - chr4.1:29667866-29667885 MS.gene52105:intron 25.0%
! AGTTGAAAACAAAATTAAGC+AGG - chr4.1:29667330-29667349 MS.gene52105:CDS 25.0%
! ATATCAATCTACCATTTCAT+AGG + chr4.1:29667741-29667760 None:intergenic 25.0%
! ATTCTATGATTTCATTGCTT+TGG - chr4.1:29667695-29667714 MS.gene52105:intron 25.0%
! CATTCAATAAATGATGTATG+TGG + chr4.1:29667464-29667483 None:intergenic 25.0%
! CATTCAATAAATGTTGTATG+TGG - chr4.1:29667425-29667444 MS.gene52105:intron 25.0%
! GTTGAAAACAAAATTAAGCA+GGG - chr4.1:29667331-29667350 MS.gene52105:CDS 25.0%
! TTTATGACTGAATTGAATTC+AGG - chr4.1:29668461-29668480 MS.gene52105:CDS 25.0%
!! AATTTGCTTATAATAGTGAC+CGG - chr4.1:29667499-29667518 MS.gene52105:intron 25.0%
!! AATTTGCTTATAATAGTGAC+CGG + chr4.1:29667518-29667499 None:intergenic 25.0%
!! TAAACCGATATGTTTTATGT+TGG - chr4.1:29668668-29668687 MS.gene52105:intron 25.0%
!! TTCATTGTGTTTGATTTCTT+AGG - chr4.1:29666684-29666703 MS.gene52105:CDS 25.0%
!!! TTTGTCTTCATTTTTAACAG+TGG + chr4.1:29668558-29668577 None:intergenic 25.0%
AAAGGAAAAATCAGCAATCT+TGG + chr4.1:29668218-29668237 None:intergenic 30.0%
AAGCCTATTAATGTGAAGTA+TGG - chr4.1:29668138-29668157 MS.gene52105:intron 30.0%
AAGGAAAAATCAGCAATCTT+GGG + chr4.1:29668217-29668236 None:intergenic 30.0%
AATCCCATACTGTATTGTTA+AGG - chr4.1:29668384-29668403 MS.gene52105:CDS 30.0%
AGTATGCAATAAGCTTCAAA+TGG + chr4.1:29667363-29667382 None:intergenic 30.0%
ATCCCATACTGTATTGTTAA+GGG - chr4.1:29668385-29668404 MS.gene52105:CDS 30.0%
TAATCTCTCTACACTTAAAC+AGG - chr4.1:29668496-29668515 MS.gene52105:CDS 30.0%
TCGAAAATCGTAAATGAGAT+TGG + chr4.1:29666779-29666798 None:intergenic 30.0%
! AACAAAATTAAGCAGGGTTT+TGG - chr4.1:29667337-29667356 MS.gene52105:CDS 30.0%
! TGATAAGTACTGTGAAGAAA+TGG + chr4.1:29666638-29666657 None:intergenic 30.0%
!! GCATTTGTTTTCAAATTTGC+AGG - chr4.1:29666831-29666850 MS.gene52105:CDS 30.0%
AAAACCCTCGACAAAAATCT+AGG - chr4.1:29667033-29667052 MS.gene52105:CDS 35.0%
AAATGAGATTGGAATTGACG+CGG + chr4.1:29666768-29666787 None:intergenic 35.0%
AAGCCAGACAACTAATTCAA+TGG + chr4.1:29668341-29668360 None:intergenic 35.0%
AAGTATCTTGAACAAGCTTG+TGG + chr4.1:29668025-29668044 None:intergenic 35.0%
ACAACAAACTTACACTTCCT+AGG + chr4.1:29668420-29668439 None:intergenic 35.0%
AGCCAGACAACTAATTCAAT+GGG + chr4.1:29668340-29668359 None:intergenic 35.0%
AGGCCATACTTCACATTAAT+AGG + chr4.1:29668144-29668163 None:intergenic 35.0%
ATACTGTATTGTTAAGGGCA+AGG - chr4.1:29668390-29668409 MS.gene52105:CDS 35.0%
ATTTCTTAGGCTCCCAAAAA+AGG - chr4.1:29666697-29666716 MS.gene52105:CDS 35.0%
CGTTAGGTTATATTAGAGTC+TGG - chr4.1:29667882-29667901 MS.gene52105:intron 35.0%
GAGGCAGTACTTTATTAGTT+TGG - chr4.1:29667904-29667923 MS.gene52105:intron 35.0%
TAGGTTATATTAGAGTCTGG+AGG - chr4.1:29667885-29667904 MS.gene52105:intron 35.0%
TGCCCTTAACAATACAGTAT+GGG + chr4.1:29668390-29668409 None:intergenic 35.0%
TTAATATAGGATTTCGGCCT+TGG - chr4.1:29667168-29667187 MS.gene52105:intron 35.0%
TTGCCCTTAACAATACAGTA+TGG + chr4.1:29668391-29668410 None:intergenic 35.0%
! AAACAGCAGCAGTTTTCTTA+TGG + chr4.1:29668526-29668545 None:intergenic 35.0%
! ACTGGTTTCTTCCTATGAAA+TGG - chr4.1:29667727-29667746 MS.gene52105:intron 35.0%
! GTAGAGAAGCTTTTTATCCA+AGG + chr4.1:29667188-29667207 None:intergenic 35.0%
! GTTGCTTTCATAAGCTATCT+TGG - chr4.1:29667291-29667310 MS.gene52105:CDS 35.0%
! GTTTTCAAATTTGCAGGAGA+AGG - chr4.1:29666837-29666856 MS.gene52105:CDS 35.0%
! TTGCTTTGGTTTCTTTCTAC+TGG - chr4.1:29667709-29667728 MS.gene52105:intron 35.0%
!! TCAAGATACTTCTGAAGTAC+AGG - chr4.1:29668034-29668053 MS.gene52105:intron 35.0%
!!! GGAGCTTTAACACCTTTTTT+GGG + chr4.1:29666712-29666731 None:intergenic 35.0%
!!! TGGAGCTTTAACACCTTTTT+TGG + chr4.1:29666713-29666732 None:intergenic 35.0%
AAAGGAAAGGCTTATTGCCA+AGG - chr4.1:29668788-29668807 MS.gene52105:intron 40.0%
AAGACAAAATGGAGTTCAGC+AGG - chr4.1:29668570-29668589 MS.gene52105:intron 40.0%
AATAAGCCTTTCCTTTGCCT+TGG + chr4.1:29668784-29668803 None:intergenic 40.0%
AATCTGGTCAAATCCCTCTT+CGG + chr4.1:29666922-29666941 None:intergenic 40.0%
ACAAACTTACACTTCCTAGG+CGG + chr4.1:29668417-29668436 None:intergenic 40.0%
ACCCCATTGAATTAGTTGTC+TGG - chr4.1:29668335-29668354 MS.gene52105:intron 40.0%
AGACAAAATGGAGTTCAGCA+GGG - chr4.1:29668571-29668590 MS.gene52105:intron 40.0%
ATTTGACCAGATTCGTGAAG+TGG - chr4.1:29666929-29666948 MS.gene52105:intron 40.0%
CCTCCAATTCCAAATTGCTT+TGG + chr4.1:29666889-29666908 None:intergenic 40.0%
GCCAGACAACTAATTCAATG+GGG + chr4.1:29668339-29668358 None:intergenic 40.0%
TAATAACCACTTACTCCCTC+CGG + chr4.1:29667521-29667540 None:intergenic 40.0%
TGAGGTAGGTAACATGATTG+AGG + chr4.1:29668164-29668183 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr4.1:29667503-29667522 MS.gene52105:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr4.1:29667522-29667503 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr4.1:29667502-29667521 MS.gene52105:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr4.1:29667521-29667502 None:intergenic 40.0%
! ATGACAAGTATGAGGAGTAC+AGG - chr4.1:29668712-29668731 MS.gene52105:intron 40.0%
! CCAAAGCAATTTGGAATTGG+AGG - chr4.1:29666886-29666905 MS.gene52105:CDS 40.0%
! CGTCCAAAGCAATTTGGAAT+TGG - chr4.1:29666883-29666902 MS.gene52105:CDS 40.0%
! CTGACCTAGATTTTTGTCGA+GGG + chr4.1:29667040-29667059 None:intergenic 40.0%
! GCTTTAAGCTACAACGTGTA+TGG + chr4.1:29667817-29667836 None:intergenic 40.0%
! TCTGACCTAGATTTTTGTCG+AGG + chr4.1:29667041-29667060 None:intergenic 40.0%
! TTAGTGAACTGGTTCAAAGC+TGG + chr4.1:29667015-29667034 None:intergenic 40.0%
!! AGAAGGAGAGGCTTTTGAAA+AGG - chr4.1:29668076-29668095 MS.gene52105:intron 40.0%
!! GAAGGAGAGGCTTTTGAAAA+GGG - chr4.1:29668077-29668096 MS.gene52105:intron 40.0%
!! TTCTTCTTTGAAGCTGCAAC+TGG + chr4.1:29666733-29666752 None:intergenic 40.0%
!!! AGAAGAGGATTTTGAAGCAG+AGG - chr4.1:29666977-29666996 MS.gene52105:intron 40.0%
!!! GATTTTGAAGCAGAGGTTGA+AGG - chr4.1:29666984-29667003 MS.gene52105:intron 40.0%
AGCTTATTGCATACTCCCTC+CGG - chr4.1:29667368-29667387 MS.gene52105:CDS 45.0%
CAAACTTACACTTCCTAGGC+GGG + chr4.1:29668416-29668435 None:intergenic 45.0%
GAATTGACGCGGTTACTTAC+AGG + chr4.1:29666757-29666776 None:intergenic 45.0%
GATTTCCATCTTCCTGCACA+AGG + chr4.1:29668368-29668387 None:intergenic 45.0%
TGCATGTGCTGCATAAATGC+TGG - chr4.1:29667670-29667689 MS.gene52105:intron 45.0%
TTTGGACGCTTCTCGAATAG+CGG + chr4.1:29666871-29666890 None:intergenic 45.0%
! ATGAGGAGTACAGGAAGAAG+TGG - chr4.1:29668721-29668740 MS.gene52105:intron 45.0%
! TGAGGAGTACAGGAAGAAGT+GGG - chr4.1:29668722-29668741 MS.gene52105:intron 45.0%
!! GAGAAGCGTCCAAAGCAATT+TGG - chr4.1:29666877-29666896 MS.gene52105:CDS 45.0%
!! TGTCGAGGGTTTTAGTGAAC+TGG + chr4.1:29667026-29667045 None:intergenic 45.0%
ACAAGGCTGAGAAGAAGGAG+AGG - chr4.1:29668064-29668083 MS.gene52105:intron 50.0%
ATGCCACCTTAATCGCCTCT+AGG + chr4.1:29668598-29668617 None:intergenic 50.0%
CAAATCCCAAGCCAAGACCA+AGG - chr4.1:29668764-29668783 MS.gene52105:intron 50.0%
CCAAGACCAAGGCAAAGGAA+AGG - chr4.1:29668775-29668794 MS.gene52105:intron 50.0%
CCTTTCCTTTGCCTTGGTCT+TGG + chr4.1:29668778-29668797 None:intergenic 50.0%
CTCTGCACCTGAACGTTCTT+CGG + chr4.1:29666955-29666974 None:intergenic 50.0%
GAAAGACAACCTGCTCGATG+AGG + chr4.1:29668182-29668201 None:intergenic 50.0%
GGAAAGGCTTATTGCCAAGG+AGG - chr4.1:29668791-29668810 MS.gene52105:intron 50.0%
GTGAGCAATCACGACAAGCT+GGG + chr4.1:29668308-29668327 None:intergenic 50.0%
TGAGGACAAGGCTGAGAAGA+AGG - chr4.1:29668059-29668078 MS.gene52105:intron 50.0%
TGTTACCTACCTCATCGAGC+AGG - chr4.1:29668170-29668189 MS.gene52105:intron 50.0%
TTCAGGTGCAGAGGAAGAAG+AGG - chr4.1:29666962-29666981 MS.gene52105:intron 50.0%
TTGGACGCTTCTCGAATAGC+GGG + chr4.1:29666870-29666889 None:intergenic 50.0%
TTTGAAAAGGGCTCAGGCAG+AGG - chr4.1:29668089-29668108 MS.gene52105:intron 50.0%
! GAGGAGTACAGGAAGAAGTG+GGG - chr4.1:29668723-29668742 MS.gene52105:intron 50.0%
! GTGAACTGGTTCAAAGCTGG+TGG + chr4.1:29667012-29667031 None:intergenic 50.0%
! TTCTTCTCAGCCTTGTCCTC+AGG + chr4.1:29668060-29668079 None:intergenic 50.0%
!! AAGTGGGGTGGTGGAATCAT+GGG - chr4.1:29668738-29668757 MS.gene52105:intron 50.0%
!! ACTTCTGAAGTACAGGCCTG+AGG - chr4.1:29668041-29668060 MS.gene52105:intron 50.0%
!! CTTGGTCTTGGCTTGGGATT+TGG + chr4.1:29668766-29668785 None:intergenic 50.0%
!! CTTTGCCTTGGTCTTGGCTT+GGG + chr4.1:29668772-29668791 None:intergenic 50.0%
!! GAGGCTTTTGAAAAGGGCTC+AGG - chr4.1:29668083-29668102 MS.gene52105:intron 50.0%
CAGGGTCCTAGAGGCGATTA+AGG - chr4.1:29668589-29668608 MS.gene52105:intron 55.0%
CCAAGCCAAGACCAAGGCAA+AGG - chr4.1:29668770-29668789 MS.gene52105:intron 55.0%
CGAAGAACGTTCAGGTGCAG+AGG - chr4.1:29666953-29666972 MS.gene52105:intron 55.0%
CGTGAGCAATCACGACAAGC+TGG + chr4.1:29668309-29668328 None:intergenic 55.0%
CTGGTCAAATCCCTCTTCGG+CGG + chr4.1:29666919-29666938 None:intergenic 55.0%
CTTCGGCCACTTCACGAATC+TGG + chr4.1:29666938-29666957 None:intergenic 55.0%
GAACAAGCTTGTGGCTGCAG+AGG + chr4.1:29668016-29668035 None:intergenic 55.0%
GAAGTACAGGCCTGAGGACA+AGG - chr4.1:29668047-29668066 MS.gene52105:intron 55.0%
GAAGTGGCCGAAGAACGTTC+AGG - chr4.1:29666945-29666964 MS.gene52105:intron 55.0%
GACAACCTGCTCGATGAGGT+AGG + chr4.1:29668178-29668197 None:intergenic 55.0%
GAGTACAGGAAGAAGTGGGG+TGG - chr4.1:29668726-29668745 MS.gene52105:intron 55.0%
GGTCCTAGAGGCGATTAAGG+TGG - chr4.1:29668592-29668611 MS.gene52105:intron 55.0%
TACAGGAAGAAGTGGGGTGG+TGG - chr4.1:29668729-29668748 MS.gene52105:intron 55.0%
TAGTGACCGGAGGGAGTAAG+TGG - chr4.1:29667512-29667531 MS.gene52105:intron 55.0%
TCCATCTTCCTGCACAAGGC+TGG + chr4.1:29668364-29668383 None:intergenic 55.0%
! TCCAGCCTTGTGCAGGAAGA+TGG - chr4.1:29668360-29668379 MS.gene52105:intron 55.0%
!! ACGCTTCTCGAATAGCGGGT+TGG + chr4.1:29666866-29666885 None:intergenic 55.0%
!! CCTTTGCCTTGGTCTTGGCT+TGG + chr4.1:29668773-29668792 None:intergenic 55.0%
!! GAAGTGGGGTGGTGGAATCA+TGG - chr4.1:29668737-29668756 MS.gene52105:intron 55.0%
AGGTGCATTGCCGCCGAAGA+GGG - chr4.1:29666906-29666925 MS.gene52105:intron 60.0%
CATTCTCTGGGCAGCCTCCT+TGG + chr4.1:29668808-29668827 None:intergenic 60.0%
GGAGTTCAGCAGGGTCCTAG+AGG - chr4.1:29668580-29668599 MS.gene52105:intron 60.0%
! TCTGGCTTCCAGCCTTGTGC+AGG - chr4.1:29668353-29668372 MS.gene52105:intron 60.0%
AAGGGCTCAGGCAGAGGCTG+AGG - chr4.1:29668095-29668114 MS.gene52105:intron 65.0%
AGGGCTCAGGCAGAGGCTGA+GGG - chr4.1:29668096-29668115 MS.gene52105:intron 65.0%
GAGGTGCATTGCCGCCGAAG+AGG - chr4.1:29666905-29666924 MS.gene52105:intron 65.0%
GTTAAGGGCAAGGCCCGCCT+AGG - chr4.1:29668400-29668419 MS.gene52105:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 29666593 29668833 29666593 ID=MS.gene52105
chr4.1 mRNA 29666593 29668833 29666593 ID=MS.gene52105.t1;Parent=MS.gene52105
chr4.1 exon 29668831 29668833 29668831 ID=MS.gene52105.t1.exon1;Parent=MS.gene52105.t1
chr4.1 CDS 29668831 29668833 29668831 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
chr4.1 exon 29668670 29668720 29668670 ID=MS.gene52105.t1.exon2;Parent=MS.gene52105.t1
chr4.1 CDS 29668670 29668720 29668670 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
chr4.1 exon 29668372 29668573 29668372 ID=MS.gene52105.t1.exon3;Parent=MS.gene52105.t1
chr4.1 CDS 29668372 29668573 29668372 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
chr4.1 exon 29667235 29667404 29667235 ID=MS.gene52105.t1.exon4;Parent=MS.gene52105.t1
chr4.1 CDS 29667235 29667404 29667235 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
chr4.1 exon 29667000 29667128 29667000 ID=MS.gene52105.t1.exon5;Parent=MS.gene52105.t1
chr4.1 CDS 29667000 29667128 29667000 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
chr4.1 exon 29666816 29666908 29666816 ID=MS.gene52105.t1.exon6;Parent=MS.gene52105.t1
chr4.1 CDS 29666816 29666908 29666816 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
chr4.1 exon 29666593 29666724 29666593 ID=MS.gene52105.t1.exon7;Parent=MS.gene52105.t1
chr4.1 CDS 29666593 29666724 29666593 ID=cds.MS.gene52105.t1;Parent=MS.gene52105.t1
Gene Sequence

>MS.gene52105

ATGGTGCGTAAACTCTATCTCAATTCAGATCTTCGTTTATCTCCATTTCTTCACAGTACTTATCAATTTTTGTCTCTAATTAACGTTTATCTTCATTGTGTTTGATTTCTTAGGCTCCCAAAAAAGGTGTTAAAGCTCCAGTTGCAGCTTCAAAGAAGAAACCTGTAAGTAACCGCGTCAATTCCAATCTCATTTACGATTTTCGATTCATTTTTTGTATTGTTTTGAAAATGAATTTGCATTTGTTTTCAAATTTGCAGGAGAAGGTTACCAACCCGCTATTCGAGAAGCGTCCAAAGCAATTTGGAATTGGAGGTGCATTGCCGCCGAAGAGGGATTTGACCAGATTCGTGAAGTGGCCGAAGAACGTTCAGGTGCAGAGGAAGAAGAGGATTTTGAAGCAGAGGTTGAAGGTTCCACCAGCTTTGAACCAGTTCACTAAAACCCTCGACAAAAATCTAGGTCAGAAATAAATTATTGTAATGTTATGTTTGGATTTGATTTTGACTGCTATTAGAGTTATTTATGTGTTATTTGCAATTTTTGTAATATGTTAATTGTGGTAAATTGTAAAATTAATATAGGATTTCGGCCTTGGATAAAAAGCTTCTCTACAGCTAATAGCATAAGTGCTTATTTGTATAAGCTATTTCTATAGCAAAAGATAAAACAAAGTTCAATTATTTTTGTATATATAAGTTGCTTTCATAAGCTATCTTGGTGCTCTCAATGAAATAAGTTGAAAACAAAATTAAGCAGGGTTTTGGCCATTTGAAGCTTATTGCATACTCCCTCCGGTCACTATTATAAGCAAATTTCTACTTTTTAGATTCATTCAATAAATGTTGTATGTGGTCTACAATATAGACCACATACATCATTTATTGAATGAACCTAAAAAGTCAAAATTTGCTTATAATAGTGACCGGAGGGAGTAAGTGGTTATTATGATTATCAGCTGTTTGTATAAACTAAAGTTAAGTTGTTTTTGTATAAGCTATTAGCTGTTTTGATAACTTCTCCCATTTAAGCTTTTGAAATAACTTATCAATGCTCATGAAATTAGCTTGATAACTTTGCATGTGCTGCATAAATGCTGGTAATTCTATGATTTCATTGCTTTGGTTTCTTTCTACTGGTTTCTTCCTATGAAATGGTAGATTGATATGCGAGTATGCTGTTACTTTGACAGTTTTTGTTATCTCAAACGTTATTTTACTGCCATACACGTTGTAGCTTAAAGCACTGCTAAGCATTTTACTTGTTTGTTTTGACGTTAAACAATTATTCGTTAGGTTATATTAGAGTCTGGAGGCAGTACTTTATTAGTTTGGTTCCCTGATATAACAATATACTGATTCTTTGTTCATTTTTGTTGCTTTATATTTAATGTGATTTTTTATCATCTTTGATTGTCGTACCTCTGCAGCCACAAGCTTGTTCAAGATACTTCTGAAGTACAGGCCTGAGGACAAGGCTGAGAAGAAGGAGAGGCTTTTGAAAAGGGCTCAGGCAGAGGCTGAGGGCAAAACAGTTGAAACTAAGAAGCCTATTAATGTGAAGTATGGCCTCAATCATGTTACCTACCTCATCGAGCAGGTTGTCTTTCTGTCATGTTACTCCCAAGATTGCTGATTTTTCCTTTTAATTTGTTATTTTACTGTGATATTGATGAATGACTGATTAAGCTTTATTTTCTTTACAGAATAAAGCCCAGCTTGTCGTGATTGCTCACGATGTCGACCCCATTGAATTAGTTGTCTGGCTTCCAGCCTTGTGCAGGAAGATGGAAATCCCATACTGTATTGTTAAGGGCAAGGCCCGCCTAGGAAGTGTAAGTTTGTTGTGATTTGAATACCTATATATGATTTATGACTGAATTGAATTCAGGTGATGACTAATTTAATCTCTCTACACTTAAACAGGTTGTCCATAAGAAAACTGCTGCTGTTTTGTGCTTGACCACTGTTAAAAATGAAGACAAAATGGAGTTCAGCAGGGTCCTAGAGGCGATTAAGGTGGCATATAATTTTATTTTCATTTTTGTTCTCTACTATTTATATACTCTTGCCATTTAAACCGATATGTTTTATGTTGGTTTTATTTCAGGCAAATTTCAATGACAAGTATGAGGAGTACAGGAAGAAGTGGGGTGGTGGAATCATGGGTTCCAAATCCCAAGCCAAGACCAAGGCAAAGGAAAGGCTTATTGCCAAGGAGGCTGCCCAGAGAATGACCTAG
Protein sequence

>MS.gene52105.t1

MAPKKGVKAPVAASKKKPEKVTNPLFEKRPKQFGIGGALPPKRDLTRFVKWPKNVQVQRKKRILKQRLKVPPALNQFTKTLDKNLATSLFKILLKYRPEDKAEKKERLLKRAQAEAEGKTVETKKPINVKYGLNHVTYLIEQNKAQLVVIAHDVDPIELVVWLPALCRKMEIPYCIVKGKARLGSVVHKKTAAVLCLTTVKNEDKMEFSRVLEAIKANFNDKYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT