AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53546

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53546.t1 MTR_1g045410 99.261 406 3 0 1 406 1 406 0.0 823
MS.gene53546.t1 MTR_5g025120 95.500 400 18 0 7 406 9 408 0.0 744
MS.gene53546.t1 MTR_1g066990 93.564 404 26 0 3 406 2 405 0.0 737
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53546.t1 AT3G09630 83.005 406 69 0 1 406 1 406 0.0 695
MS.gene53546.t1 AT3G09630 82.759 406 69 1 1 406 1 405 0.0 688
MS.gene53546.t1 AT5G02870 82.759 406 70 0 1 406 2 407 0.0 686
MS.gene53546.t1 AT5G02870 82.512 406 70 1 1 406 2 406 0.0 679

Find 124 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGACCGTTGATTGTTTA+TGG 0.172085 1.2:+28346278 MS.gene53546:CDS
CGACCAGAATCTGGCGTAAA+TGG 0.252089 1.2:+28345976 MS.gene53546:CDS
CCTGAATTTCCTCTTGTTGT+TGG 0.273532 1.2:+28346107 MS.gene53546:CDS
GAGAAACTTGATTCGATTTA+CGG 0.289997 1.2:+28346440 MS.gene53546:CDS
GTGCCATTTACGCCAGATTC+TGG 0.296253 1.2:-28345979 None:intergenic
CGAAAGCGGTGAAAGCATTT+AGG 0.315122 1.2:+28346312 MS.gene53546:CDS
CTTGCACCTGGTGGGCATTT+GGG 0.316137 1.2:+28346386 MS.gene53546:CDS
CCCATGACTCGGCGGAGGTT+TGG 0.322077 1.2:-28345845 None:intergenic
CGCTTAACATCCTTCTTAAT+CGG 0.329357 1.2:-28346578 None:intergenic
ACTTGCACCTGGTGGGCATT+TGG 0.330852 1.2:+28346385 MS.gene53546:CDS
GGAGGAAGCTTCTGCCATTA+AGG 0.333983 1.2:+28348305 MS.gene53546:intron
AGGTTTGGTGGCCGGCTTTA+CGG 0.335499 1.2:-28345830 None:intergenic
GTGGACGAAGTTGACGATAT+CGG 0.344158 1.2:-28345766 None:intergenic
GAAGCGTTACGCCGTTGTTT+CGG 0.347922 1.2:+28346022 MS.gene53546:CDS
ATTCCTCGTGTCGCCGGAGG+TGG 0.359384 1.2:+28345891 MS.gene53546:CDS
CGTGCTGGTCAAGCTGCTTT+CGG 0.369434 1.2:+28345921 MS.gene53546:CDS
TTGTTCTAGCTCGTGGTCAT+AGG 0.374699 1.2:+28346072 MS.gene53546:CDS
GAAGAGGAAGACTGTTTCTA+AGG 0.381777 1.2:+28346736 MS.gene53546:CDS
ATTTGGGTAGGTTTGTTGTT+TGG 0.382908 1.2:+28346402 MS.gene53546:CDS
TGTTTGCTCCGACCAGAATC+TGG 0.383149 1.2:+28345967 MS.gene53546:CDS
AGAAACTTGATTCGATTTAC+GGG 0.384012 1.2:+28346441 MS.gene53546:CDS
CCAACAACAAGAGGAAATTC+AGG 0.384867 1.2:-28346107 None:intergenic
GACGAGGGGTCTTGCGGCGG+CGG 0.388200 1.2:-28345658 None:intergenic
CTCTTAGCAGTCTTAGCATA+AGG 0.391556 1.2:-28346656 None:intergenic
GTTTGTTGTTTGGACTAAAT+CGG 0.397101 1.2:+28346412 MS.gene53546:CDS
TGGTAGGGTGGTTGATGAAT+CGG 0.402450 1.2:-28345712 None:intergenic
AGAAAGCGAAAGCGAGTATT+GGG 0.418116 1.2:+28346207 MS.gene53546:CDS
GACAACTTCTCCAAGTGGTT+GGG 0.425629 1.2:+28348384 MS.gene53546:CDS
TGGACGAAGTTGACGATATC+GGG 0.426113 1.2:-28345765 None:intergenic
CCATGTCGGAGTCGAGTGTT+TGG 0.427372 1.2:-28345686 None:intergenic
CTCGGCGGAGGTTTGGTGGC+CGG 0.427401 1.2:-28345838 None:intergenic
TGTTTCGGCTATCGCGGCTT+CGG 0.431114 1.2:+28346037 MS.gene53546:CDS
ATAACATTAAGATTCTTAAG+AGG 0.432087 1.2:-28346620 None:intergenic
CTCCGGCGACACGAGGAATA+CGG 0.433390 1.2:-28345887 None:intergenic
CCAAACCTCCGCCGAGTCAT+GGG 0.437310 1.2:+28345845 MS.gene53546:CDS
AGCACGGTGGGTCCCACCTC+CGG 0.442516 1.2:-28345904 None:intergenic
GCATTACATCGGGGATTGGT+AGG 0.449379 1.2:-28345728 None:intergenic
TGCTGAGGCTGAGAGGGTTA+AGG 0.451803 1.2:+28346691 MS.gene53546:CDS
GGCTGACGGCGTATGGTTGT+CGG 0.459070 1.2:-28345809 None:intergenic
ATTGAAGCTCGCATTACATC+GGG 0.459128 1.2:-28345738 None:intergenic
TCCGCCGAGTCATGGGGAAC+TGG 0.459755 1.2:+28345852 MS.gene53546:CDS
ACCAAACCTCCGCCGAGTCA+TGG 0.466870 1.2:+28345844 MS.gene53546:CDS
TTTCACCGCTTTCGCGCCTT+CGG 0.470737 1.2:-28346303 None:intergenic
GCTTTACGGCTGACGGCGTA+TGG 0.481452 1.2:-28345816 None:intergenic
CACCTGGTGGGCATTTGGGT+AGG 0.482518 1.2:+28346390 MS.gene53546:CDS
GAGAAAGCGAAAGCGAGTAT+TGG 0.484167 1.2:+28346206 MS.gene53546:CDS
GCTGACGGCGTATGGTTGTC+GGG 0.485665 1.2:-28345808 None:intergenic
TCCCGTATTCCTCGTGTCGC+CGG 0.485883 1.2:+28345885 MS.gene53546:CDS
GCTTCTGCCATTAAGGCAGC+TGG 0.495195 1.2:+28348312 MS.gene53546:CDS
TGAGAGGGTTAAGGCTAAGA+AGG 0.498124 1.2:+28346700 MS.gene53546:CDS
TGACAACTTCTCCAAGTGGT+TGG 0.498943 1.2:+28348383 MS.gene53546:CDS
CTTTGCTTGCTGAGGCTGAG+AGG 0.509740 1.2:+28346684 MS.gene53546:CDS
ATGACTCGGCGGAGGTTTGG+TGG 0.511760 1.2:-28345842 None:intergenic
GTTGTTGGTGATTCAGCTGA+AGG 0.512405 1.2:+28346122 MS.gene53546:CDS
AAAAGCTTGGTACAACACTA+TGG 0.513409 1.2:+28348335 MS.gene53546:CDS
CATTACATCGGGGATTGGTA+GGG 0.514894 1.2:-28345727 None:intergenic
GCTCGCATTACATCGGGGAT+TGG 0.515117 1.2:-28345732 None:intergenic
TTTGCTTGCTGAGGCTGAGA+GGG 0.515410 1.2:+28346685 MS.gene53546:CDS
TCCGGCGACACGAGGAATAC+GGG 0.515486 1.2:-28345886 None:intergenic
GAGGATGGCTTTGCTTGCTG+AGG 0.523283 1.2:+28346676 MS.gene53546:CDS
GACGGTGACGAGGGGTCTTG+CGG 0.525418 1.2:-28345664 None:intergenic
TACGCCGTCAGCCGTAAAGC+CGG 0.525800 1.2:+28345819 MS.gene53546:CDS
TACATCGGGGATTGGTAGGG+TGG 0.529351 1.2:-28345724 None:intergenic
GATTGAAGCTCGCATTACAT+CGG 0.530724 1.2:-28345739 None:intergenic
CGTAGGTATATATCGCGAAA+AGG 0.530754 1.2:+28346260 MS.gene53546:CDS
AACCTACCCAAATGCCCACC+AGG 0.531845 1.2:-28346392 None:intergenic
CGAGTGTTTGGACGGTGACG+AGG 0.534152 1.2:-28345674 None:intergenic
TTCCTCGTGTCGCCGGAGGT+GGG 0.534567 1.2:+28345892 MS.gene53546:CDS
TGAAGGAGTTGAGAAAACTA+AGG 0.535005 1.2:+28346139 MS.gene53546:CDS
TGTTGTGAGGCCGATTAAGA+AGG 0.535007 1.2:+28346568 MS.gene53546:CDS
CGCCGTTGTTTCGGCTATCG+CGG 0.536077 1.2:+28346031 MS.gene53546:CDS
AGCGAGTATTGGGATTCGAC+CGG 0.536233 1.2:+28346217 MS.gene53546:CDS
AGTTCCCCATGACTCGGCGG+AGG 0.537274 1.2:-28345850 None:intergenic
AGGAGAAGCTTGATAAGAAG+AGG 0.538354 1.2:+28346720 MS.gene53546:CDS
GCGAGTATTGGGATTCGACC+GGG 0.541420 1.2:+28346218 MS.gene53546:CDS
GGAGGTGGGACCCACCGTGC+TGG 0.542028 1.2:+28345906 MS.gene53546:CDS
TGGTTAATTCTGATTTGACT+AGG 0.548923 1.2:+28346519 MS.gene53546:CDS
CCATCGCTTGTTCTAGCTCG+TGG 0.549509 1.2:+28346065 MS.gene53546:CDS
TTAAGGCAGCTGGAAAAGCT+TGG 0.555525 1.2:+28348322 MS.gene53546:CDS
AGCAGCTTGACCAGCACGGT+GGG 0.557276 1.2:-28345916 None:intergenic
CTGTGAAACACCCAACCACT+TGG 0.559270 1.2:-28348394 None:intergenic
ATTGGGATTCGACCGGGGAA+AGG 0.562650 1.2:+28346224 MS.gene53546:CDS
GAATTAACCATCTTAGCCCT+AGG 0.569796 1.2:-28346506 None:intergenic
TTGATTGTTTATGGTACCGA+AGG 0.570169 1.2:+28346287 MS.gene53546:CDS
CATTTACGCCAGATTCTGGT+CGG 0.570290 1.2:-28345975 None:intergenic
TTACGCATCTTACCTTTCCC+CGG 0.571177 1.2:-28346236 None:intergenic
GTGGCCGGCTTTACGGCTGA+CGG 0.574691 1.2:-28345823 None:intergenic
TGATGAATCGGTGGCCATGT+CGG 0.575317 1.2:-28345700 None:intergenic
GTCGGAGTCGAGTGTTTGGA+CGG 0.584029 1.2:-28345682 None:intergenic
GAATCTGGCGTAAATGGCAC+CGG 0.584056 1.2:+28345982 MS.gene53546:CDS
CTTATGCTAAGACTGCTAAG+AGG 0.584978 1.2:+28346657 MS.gene53546:CDS
TTGGTACAACACTATGGTCT+CGG 0.586235 1.2:+28348341 MS.gene53546:CDS
GCTGAATCACCAACAACAAG+AGG 0.587185 1.2:-28346116 None:intergenic
GGTGACGAGGGGTCTTGCGG+CGG 0.587727 1.2:-28345661 None:intergenic
TGCTAAGACTGCTAAGAGGA+TGG 0.588122 1.2:+28346661 MS.gene53546:CDS
GAGTGTTTGGACGGTGACGA+GGG 0.598147 1.2:-28345673 None:intergenic
CGTATTCCTCGTGTCGCCGG+AGG 0.600022 1.2:+28345888 MS.gene53546:CDS
GACACGAGGAATACGGGAGA+CGG 0.603426 1.2:-28345880 None:intergenic
GTCCCACCTCCGGCGACACG+AGG 0.606474 1.2:-28345894 None:intergenic
ACGACCAGTTCCCCATGACT+CGG 0.621428 1.2:-28345856 None:intergenic
AAGGTAAGATGCGTAACCGT+AGG 0.626245 1.2:+28346243 MS.gene53546:CDS
AATTTGACAACTTCTCCAAG+TGG 0.627357 1.2:+28348379 MS.gene53546:CDS
TTGAGATTACTAATGTTGAG+AGG 0.628464 1.2:+28346348 MS.gene53546:CDS
TCGGTACCATAAACAATCAA+CGG 0.638403 1.2:-28346284 None:intergenic
CCAAACACTCGACTCCGACA+TGG 0.639783 1.2:+28345686 MS.gene53546:CDS
TGGTACCGAAGGCGCGAAAG+CGG 0.639834 1.2:+28346298 MS.gene53546:CDS
TTCGGTAACATGTGCCGTGG+TGG 0.641371 1.2:+28345939 MS.gene53546:CDS
CAAACCTCCGCCGAGTCATG+GGG 0.643143 1.2:+28345846 MS.gene53546:CDS
CCACGAGCTAGAACAAGCGA+TGG 0.643292 1.2:-28346065 None:intergenic
TAGGATTATTAACAGTGATG+AGG 0.647484 1.2:+28346538 MS.gene53546:CDS
AGCCGCGATAGCCGAAACAA+CGG 0.650942 1.2:-28346033 None:intergenic
CGAGTATTGGGATTCGACCG+GGG 0.652213 1.2:+28346219 MS.gene53546:CDS
CGAAAGCAGCTTGACCAGCA+CGG 0.654853 1.2:-28345920 None:intergenic
TAGGGTGGTTGATGAATCGG+TGG 0.659467 1.2:-28345709 None:intergenic
AGTGTTTGGACGGTGACGAG+GGG 0.664474 1.2:-28345672 None:intergenic
AAAGCATTTAGGAATATACC+TGG 0.667521 1.2:+28346323 MS.gene53546:CDS
TTGAAGCTCGCATTACATCG+GGG 0.675972 1.2:-28345737 None:intergenic
CGAAGTTGACGATATCGGGA+CGG 0.678874 1.2:-28345761 None:intergenic
ACATTAGTAATCTCAACACC+AGG 0.679853 1.2:-28346341 None:intergenic
AAGCAGCTTGACCAGCACGG+TGG 0.692670 1.2:-28345917 None:intergenic
ACCAGTTCCCCATGACTCGG+CGG 0.694588 1.2:-28345853 None:intergenic
ATGAGGTGCAGAGTGTTGTG+AGG 0.702312 1.2:+28346555 MS.gene53546:CDS
GCTTTCGGTAACATGTGCCG+TGG 0.703272 1.2:+28345936 MS.gene53546:CDS
GAGCAAACATACGACCACCA+CGG 0.745806 1.2:-28345953 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTATGTTTTATTATT+AGG + chr1.2:28348227-28348246 MS.gene53546:intron 10.0%
!! AATCATATTCCATTTACAAA+AGG + chr1.2:28347783-28347802 MS.gene53546:intron 20.0%
!! ATAACATTAAGATTCTTAAG+AGG - chr1.2:28346623-28346642 None:intergenic 20.0%
!! ATTTGTTTCAATATTGTTCA+TGG + chr1.2:28348251-28348270 MS.gene53546:intron 20.0%
!! CAATGATAGAAATTAAAACA+AGG - chr1.2:28347443-28347462 None:intergenic 20.0%
! ACATAAAACAATTACAATGC+AGG - chr1.2:28348218-28348237 None:intergenic 25.0%
! ATACCAAACAGAAATGTAAA+TGG - chr1.2:28347934-28347953 None:intergenic 25.0%
! ATTCAAATCAAAGTGCAATA+AGG - chr1.2:28346902-28346921 None:intergenic 25.0%
! CAATTAGCACATAATTTCTA+AGG - chr1.2:28347027-28347046 None:intergenic 25.0%
! TAGCAAGTTAGATAGAAATA+TGG + chr1.2:28347815-28347834 MS.gene53546:intron 25.0%
! TTATAACGTTGTTTGATCAA+TGG + chr1.2:28347852-28347871 MS.gene53546:intron 25.0%
! TTCTTCAAATGATGCAATTA+AGG + chr1.2:28347717-28347736 MS.gene53546:intron 25.0%
! TTTCCATTTACATTTCTGTT+TGG + chr1.2:28347928-28347947 MS.gene53546:intron 25.0%
!! TATGTCTTAATGACAACTTT+TGG + chr1.2:28347993-28348012 MS.gene53546:intron 25.0%
!!! ACATTATTTTCCTTTGTTTC+AGG + chr1.2:28348284-28348303 MS.gene53546:intron 25.0%
!!! ATTAGTGTTTTGAAGAAGAT+TGG + chr1.2:28346167-28346186 MS.gene53546:CDS 25.0%
!!! GTTTTGATGTTTCATTTTGT+GGG + chr1.2:28346831-28346850 MS.gene53546:intron 25.0%
!!! TGTTTTGATGTTTCATTTTG+TGG + chr1.2:28346830-28346849 MS.gene53546:intron 25.0%
CATTAACTGCAATTCAATAG+AGG - chr1.2:28348069-28348088 None:intergenic 30.0%
GATAATCAAAAGTGAAGCAT+CGG - chr1.2:28348047-28348066 None:intergenic 30.0%
TAGGATTATTAACAGTGATG+AGG + chr1.2:28346538-28346557 MS.gene53546:CDS 30.0%
TATAGAATAGGCTGCTATTA+CGG + chr1.2:28347534-28347553 MS.gene53546:intron 30.0%
TCTATCATTGACATGTGTTT+AGG + chr1.2:28347453-28347472 MS.gene53546:intron 30.0%
TTGAGATTACTAATGTTGAG+AGG + chr1.2:28346348-28346367 MS.gene53546:CDS 30.0%
! AAAGCATTTAGGAATATACC+TGG + chr1.2:28346323-28346342 MS.gene53546:CDS 30.0%
! AGAAACTTGATTCGATTTAC+GGG + chr1.2:28346441-28346460 MS.gene53546:CDS 30.0%
! AGCTTTTGTGAACATCATTT+AGG - chr1.2:28347197-28347216 None:intergenic 30.0%
! ATTTACGGGACTTTTGATAA+GGG + chr1.2:28346455-28346474 MS.gene53546:CDS 30.0%
! GAGAAACTTGATTCGATTTA+CGG + chr1.2:28346440-28346459 MS.gene53546:CDS 30.0%
! TGGTTAATTCTGATTTGACT+AGG + chr1.2:28346519-28346538 MS.gene53546:CDS 30.0%
!! AAAAAGGGTATGTTTTACCT+AGG + chr1.2:28346489-28346508 MS.gene53546:CDS 30.0%
!! AAAAGGGTATGTTTTACCTA+GGG + chr1.2:28346490-28346509 MS.gene53546:CDS 30.0%
!! GTTTGTTGTTTGGACTAAAT+CGG + chr1.2:28346412-28346431 MS.gene53546:CDS 30.0%
!! TTATTTTCCTTTGTTTCAGG+AGG + chr1.2:28348287-28348306 MS.gene53546:intron 30.0%
!!! TCTGATTCACATTGATTTTC+CGG - chr1.2:28346004-28346023 None:intergenic 30.0%
AAAAGCTTGGTACAACACTA+TGG + chr1.2:28348335-28348354 MS.gene53546:CDS 35.0%
AACCAATCCTTATGTGACTA+AGG - chr1.2:28347888-28347907 None:intergenic 35.0%
AAGGGTAGTGAGAAGAAAAA+AGG + chr1.2:28346473-28346492 MS.gene53546:CDS 35.0%
AATTTGACAACTTCTCCAAG+TGG + chr1.2:28348379-28348398 MS.gene53546:CDS 35.0%
ACATTAGTAATCTCAACACC+AGG - chr1.2:28346344-28346363 None:intergenic 35.0%
ACCAATCCTTATGTGACTAA+GGG - chr1.2:28347887-28347906 None:intergenic 35.0%
ACTGTGATACATAACACTAG+AGG - chr1.2:28347238-28347257 None:intergenic 35.0%
AGGGTAGTGAGAAGAAAAAA+GGG + chr1.2:28346474-28346493 MS.gene53546:CDS 35.0%
AGTTAGTGCTGTCAAATAGA+TGG + chr1.2:28347507-28347526 MS.gene53546:intron 35.0%
CGCTTAACATCCTTCTTAAT+CGG - chr1.2:28346581-28346600 None:intergenic 35.0%
GTGCATAGTGTAATGTTGTT+TGG + chr1.2:28347406-28347425 MS.gene53546:intron 35.0%
TCGGTACCATAAACAATCAA+CGG - chr1.2:28346287-28346306 None:intergenic 35.0%
TCTAGTGTTATGTATCACAG+TGG + chr1.2:28347237-28347256 MS.gene53546:intron 35.0%
TGAAGGAGTTGAGAAAACTA+AGG + chr1.2:28346139-28346158 MS.gene53546:CDS 35.0%
! AAAGGACCGTTGATTGTTTA+TGG + chr1.2:28346278-28346297 MS.gene53546:CDS 35.0%
! ATAGATGGTGGCTATAGAAT+AGG + chr1.2:28347522-28347541 MS.gene53546:intron 35.0%
! ATCTCTGGACCTTTTGTAAA+TGG - chr1.2:28347795-28347814 None:intergenic 35.0%
! CTTTTCGCGATATATACCTA+CGG - chr1.2:28346262-28346281 None:intergenic 35.0%
! GATTTACGGGACTTTTGATA+AGG + chr1.2:28346454-28346473 MS.gene53546:CDS 35.0%
! TGAACTGAGTTTTACTTTGC+TGG + chr1.2:28347362-28347381 MS.gene53546:intron 35.0%
! TTCCAAGTATTGTCAACTAG+TGG + chr1.2:28347567-28347586 MS.gene53546:intron 35.0%
! TTGATTGTTTATGGTACCGA+AGG + chr1.2:28346287-28346306 MS.gene53546:CDS 35.0%
!! AGTTTTTGGAAATGTTGGAG+TGG - chr1.2:28345788-28345807 None:intergenic 35.0%
!! ATTTGGGTAGGTTTGTTGTT+TGG + chr1.2:28346402-28346421 MS.gene53546:CDS 35.0%
!! CTATCTAACTTGCTAATCTC+TGG - chr1.2:28347810-28347829 None:intergenic 35.0%
!!! AATCTTTTGAAACTTGCACC+TGG + chr1.2:28346374-28346393 MS.gene53546:CDS 35.0%
AACCAGTTGGACACTCTATT+TGG + chr1.2:28347077-28347096 MS.gene53546:intron 40.0%
AATGGCATTGCTATGAAACC+GGG - chr1.2:28347677-28347696 None:intergenic 40.0%
ACCAGTTGGACACTCTATTT+GGG + chr1.2:28347078-28347097 MS.gene53546:intron 40.0%
AGAAAGCGAAAGCGAGTATT+GGG + chr1.2:28346207-28346226 MS.gene53546:CDS 40.0%
AGGAGAAGCTTGATAAGAAG+AGG + chr1.2:28346720-28346739 MS.gene53546:CDS 40.0%
ATTGAAGCTCGCATTACATC+GGG - chr1.2:28345741-28345760 None:intergenic 40.0%
CAATCCTTATGTGACTAAGG+GGG - chr1.2:28347885-28347904 None:intergenic 40.0%
CAATGCAGGAAATAGAGCAA+AGG - chr1.2:28348204-28348223 None:intergenic 40.0%
CAATGGCATTGCTATGAAAC+CGG - chr1.2:28347678-28347697 None:intergenic 40.0%
CACCACTAGTTGACAATACT+TGG - chr1.2:28347572-28347591 None:intergenic 40.0%
CCAACAACAAGAGGAAATTC+AGG - chr1.2:28346110-28346129 None:intergenic 40.0%
CCAATCCTTATGTGACTAAG+GGG - chr1.2:28347886-28347905 None:intergenic 40.0%
CGTAGGTATATATCGCGAAA+AGG + chr1.2:28346260-28346279 MS.gene53546:CDS 40.0%
CTCTTAGCAGTCTTAGCATA+AGG - chr1.2:28346659-28346678 None:intergenic 40.0%
CTTATGCTAAGACTGCTAAG+AGG + chr1.2:28346657-28346676 MS.gene53546:CDS 40.0%
GAATTAACCATCTTAGCCCT+AGG - chr1.2:28346509-28346528 None:intergenic 40.0%
GATTGAAGCTCGCATTACAT+CGG - chr1.2:28345742-28345761 None:intergenic 40.0%
TACACTATGCACAGCAGAAT+AGG - chr1.2:28347398-28347417 None:intergenic 40.0%
TAGTGCTGTCAAATAGATGG+TGG + chr1.2:28347510-28347529 MS.gene53546:intron 40.0%
TTGGTACAACACTATGGTCT+CGG + chr1.2:28348341-28348360 MS.gene53546:CDS 40.0%
! CCTGAATTTCCTCTTGTTGT+TGG + chr1.2:28346107-28346126 MS.gene53546:CDS 40.0%
! TGTTTTACCTAGGGCTAAGA+TGG + chr1.2:28346499-28346518 MS.gene53546:CDS 40.0%
!! GAAGAGGAAGACTGTTTCTA+AGG + chr1.2:28346736-28346755 MS.gene53546:CDS 40.0%
!! GTCACATAAGGATTGGTTGT+TGG + chr1.2:28347890-28347909 MS.gene53546:intron 40.0%
!! TCGGGAGTTTTTGGAAATGT+TGG - chr1.2:28345793-28345812 None:intergenic 40.0%
!! TGAAGCATCGGTTGCAATTA+AGG - chr1.2:28348035-28348054 None:intergenic 40.0%
!!! TTTTGAAACTTGCACCTGGT+GGG + chr1.2:28346378-28346397 MS.gene53546:CDS 40.0%
AAGGTAAGATGCGTAACCGT+AGG + chr1.2:28346243-28346262 MS.gene53546:CDS 45.0%
ACACTAGAGGAAGTCTTGCT+TGG - chr1.2:28347225-28347244 None:intergenic 45.0%
ATGGCATTGCTATGAAACCG+GGG - chr1.2:28347676-28347695 None:intergenic 45.0%
CACAACAGAACGAAGCACAA+TGG - chr1.2:28347695-28347714 None:intergenic 45.0%
CATACAATCTCTGATGACCC+CGG + chr1.2:28347656-28347675 MS.gene53546:intron 45.0%
CATTTACGCCAGATTCTGGT+CGG - chr1.2:28345978-28345997 None:intergenic 45.0%
CCCCTTAGTCACATAAGGAT+TGG + chr1.2:28347883-28347902 MS.gene53546:intron 45.0%
CTTATGTGACTAAGGGGGAT+CGG - chr1.2:28347880-28347899 None:intergenic 45.0%
GAAGCTTCCTCCTGAAACAA+AGG - chr1.2:28348297-28348316 None:intergenic 45.0%
GACAACTTCTCCAAGTGGTT+GGG + chr1.2:28348384-28348403 MS.gene53546:CDS 45.0%
GAGAAAGCGAAAGCGAGTAT+TGG + chr1.2:28346206-28346225 MS.gene53546:CDS 45.0%
GCTGAATCACCAACAACAAG+AGG - chr1.2:28346119-28346138 None:intergenic 45.0%
GTGGACGAAGTTGACGATAT+CGG - chr1.2:28345769-28345788 None:intergenic 45.0%
TACATGCATGCACAACCAGT+TGG + chr1.2:28347064-28347083 MS.gene53546:intron 45.0%
TGACAACTTCTCCAAGTGGT+TGG + chr1.2:28348383-28348402 MS.gene53546:CDS 45.0%
TGAGAGGGTTAAGGCTAAGA+AGG + chr1.2:28346700-28346719 MS.gene53546:CDS 45.0%
TGCTAAGACTGCTAAGAGGA+TGG + chr1.2:28346661-28346680 MS.gene53546:CDS 45.0%
TGGACGAAGTTGACGATATC+GGG - chr1.2:28345768-28345787 None:intergenic 45.0%
TGTTGTGAGGCCGATTAAGA+AGG + chr1.2:28346568-28346587 MS.gene53546:CDS 45.0%
TTAAGGCAGCTGGAAAAGCT+TGG + chr1.2:28348322-28348341 MS.gene53546:CDS 45.0%
TTACGCATCTTACCTTTCCC+CGG - chr1.2:28346239-28346258 None:intergenic 45.0%
TTGAAGCTCGCATTACATCG+GGG - chr1.2:28345740-28345759 None:intergenic 45.0%
! AGCTTTTCCAGCTGCCTTAA+TGG - chr1.2:28348322-28348341 None:intergenic 45.0%
! CATTACATCGGGGATTGGTA+GGG - chr1.2:28345730-28345749 None:intergenic 45.0%
! GTATGGTTGTCGGGAGTTTT+TGG - chr1.2:28345802-28345821 None:intergenic 45.0%
!! CGAAAGCGGTGAAAGCATTT+AGG + chr1.2:28346312-28346331 MS.gene53546:CDS 45.0%
!! GTTGTTGGTGATTCAGCTGA+AGG + chr1.2:28346122-28346141 MS.gene53546:CDS 45.0%
!! TGGTAGGGTGGTTGATGAAT+CGG - chr1.2:28345715-28345734 None:intergenic 45.0%
!! TTGTTCTAGCTCGTGGTCAT+AGG + chr1.2:28346072-28346091 MS.gene53546:CDS 45.0%
!!! CTTTTGAAACTTGCACCTGG+TGG + chr1.2:28346377-28346396 MS.gene53546:CDS 45.0%
CGAAGTTGACGATATCGGGA+CGG - chr1.2:28345764-28345783 None:intergenic 50.0%
CGACCAGAATCTGGCGTAAA+TGG + chr1.2:28345976-28345995 MS.gene53546:CDS 50.0%
CGATCCCCCTTAGTCACATA+AGG + chr1.2:28347878-28347897 MS.gene53546:intron 50.0%
CTGTGAAACACCCAACCACT+TGG - chr1.2:28348397-28348416 None:intergenic 50.0%
GAATCTGGCGTAAATGGCAC+CGG + chr1.2:28345982-28346001 MS.gene53546:CDS 50.0%
GAGCAAACATACGACCACCA+CGG - chr1.2:28345956-28345975 None:intergenic 50.0%
GCTACTGCTCTGTATCGTAG+TGG + chr1.2:28347591-28347610 MS.gene53546:intron 50.0%
GGAGGAAGCTTCTGCCATTA+AGG + chr1.2:28348305-28348324 MS.gene53546:intron 50.0%
TGATGAATCGGTGGCCATGT+CGG - chr1.2:28345703-28345722 None:intergenic 50.0%
TGTTTGCTCCGACCAGAATC+TGG + chr1.2:28345967-28345986 MS.gene53546:CDS 50.0%
! GAAGCGTTACGCCGTTGTTT+CGG + chr1.2:28346022-28346041 MS.gene53546:CDS 50.0%
! GCATTACATCGGGGATTGGT+AGG - chr1.2:28345731-28345750 None:intergenic 50.0%
! GCCCAAATAGAGTGTCCAAC+TGG - chr1.2:28347082-28347101 None:intergenic 50.0%
! TTTGCTTGCTGAGGCTGAGA+GGG + chr1.2:28346685-28346704 MS.gene53546:CDS 50.0%
!! AGCGAGTATTGGGATTCGAC+CGG + chr1.2:28346217-28346236 MS.gene53546:CDS 50.0%
!! ATGAGGTGCAGAGTGTTGTG+AGG + chr1.2:28346555-28346574 MS.gene53546:CDS 50.0%
!! GTGCCATTTACGCCAGATTC+TGG - chr1.2:28345982-28346001 None:intergenic 50.0%
!! TAGGGTGGTTGATGAATCGG+TGG - chr1.2:28345712-28345731 None:intergenic 50.0%
AACCTACCCAAATGCCCACC+AGG - chr1.2:28346395-28346414 None:intergenic 55.0%
ACGACCAGTTCCCCATGACT+CGG - chr1.2:28345859-28345878 None:intergenic 55.0%
AGCCGCGATAGCCGAAACAA+CGG - chr1.2:28346036-28346055 None:intergenic 55.0%
AGTGTTTGGACGGTGACGAG+GGG - chr1.2:28345675-28345694 None:intergenic 55.0%
ATTGGGATTCGACCGGGGAA+AGG + chr1.2:28346224-28346243 MS.gene53546:CDS 55.0%
CCAAACACTCGACTCCGACA+TGG + chr1.2:28345686-28345705 MS.gene53546:CDS 55.0%
CCACGAGCTAGAACAAGCGA+TGG - chr1.2:28346068-28346087 None:intergenic 55.0%
CCATGTCGGAGTCGAGTGTT+TGG - chr1.2:28345689-28345708 None:intergenic 55.0%
CGAAAGCAGCTTGACCAGCA+CGG - chr1.2:28345923-28345942 None:intergenic 55.0%
GACACGAGGAATACGGGAGA+CGG - chr1.2:28345883-28345902 None:intergenic 55.0%
GAGTGTTTGGACGGTGACGA+GGG - chr1.2:28345676-28345695 None:intergenic 55.0%
GCTCGCATTACATCGGGGAT+TGG - chr1.2:28345735-28345754 None:intergenic 55.0%
GCTTCTGCCATTAAGGCAGC+TGG + chr1.2:28348312-28348331 MS.gene53546:CDS 55.0%
GCTTTCGGTAACATGTGCCG+TGG + chr1.2:28345936-28345955 MS.gene53546:CDS 55.0%
GTCGGAGTCGAGTGTTTGGA+CGG - chr1.2:28345685-28345704 None:intergenic 55.0%
TGCTGAGGCTGAGAGGGTTA+AGG + chr1.2:28346691-28346710 MS.gene53546:CDS 55.0%
TGTTTCGGCTATCGCGGCTT+CGG + chr1.2:28346037-28346056 MS.gene53546:CDS 55.0%
TTCGGTAACATGTGCCGTGG+TGG + chr1.2:28345939-28345958 MS.gene53546:CDS 55.0%
TTTCACCGCTTTCGCGCCTT+CGG - chr1.2:28346306-28346325 None:intergenic 55.0%
! ACTTGCACCTGGTGGGCATT+TGG + chr1.2:28346385-28346404 MS.gene53546:CDS 55.0%
! CGTGCTGGTCAAGCTGCTTT+CGG + chr1.2:28345921-28345940 MS.gene53546:CDS 55.0%
! CTTGCACCTGGTGGGCATTT+GGG + chr1.2:28346386-28346405 MS.gene53546:CDS 55.0%
! CTTTGCTTGCTGAGGCTGAG+AGG + chr1.2:28346684-28346703 MS.gene53546:CDS 55.0%
! GAGGATGGCTTTGCTTGCTG+AGG + chr1.2:28346676-28346695 MS.gene53546:CDS 55.0%
! TACATCGGGGATTGGTAGGG+TGG - chr1.2:28345727-28345746 None:intergenic 55.0%
!! AGGTTTGGTGGCCGGCTTTA+CGG - chr1.2:28345833-28345852 None:intergenic 55.0%
!! CCATCGCTTGTTCTAGCTCG+TGG + chr1.2:28346065-28346084 MS.gene53546:CDS 55.0%
!! CGAGTATTGGGATTCGACCG+GGG + chr1.2:28346219-28346238 MS.gene53546:CDS 55.0%
!! GCGAGTATTGGGATTCGACC+GGG + chr1.2:28346218-28346237 MS.gene53546:CDS 55.0%
AAGCAGCTTGACCAGCACGG+TGG - chr1.2:28345920-28345939 None:intergenic 60.0%
ACCAAACCTCCGCCGAGTCA+TGG + chr1.2:28345844-28345863 MS.gene53546:CDS 60.0%
AGCAGCTTGACCAGCACGGT+GGG - chr1.2:28345919-28345938 None:intergenic 60.0%
CAAACCTCCGCCGAGTCATG+GGG + chr1.2:28345846-28345865 MS.gene53546:CDS 60.0%
CCAAACCTCCGCCGAGTCAT+GGG + chr1.2:28345845-28345864 MS.gene53546:CDS 60.0%
CGAGTGTTTGGACGGTGACG+AGG - chr1.2:28345677-28345696 None:intergenic 60.0%
CTCCGGCGACACGAGGAATA+CGG - chr1.2:28345890-28345909 None:intergenic 60.0%
GCTGACGGCGTATGGTTGTC+GGG - chr1.2:28345811-28345830 None:intergenic 60.0%
GCTTTACGGCTGACGGCGTA+TGG - chr1.2:28345819-28345838 None:intergenic 60.0%
GGCTGACGGCGTATGGTTGT+CGG - chr1.2:28345812-28345831 None:intergenic 60.0%
TACGCCGTCAGCCGTAAAGC+CGG + chr1.2:28345819-28345838 MS.gene53546:CDS 60.0%
TCCCGTATTCCTCGTGTCGC+CGG + chr1.2:28345885-28345904 MS.gene53546:CDS 60.0%
TCCGGCGACACGAGGAATAC+GGG - chr1.2:28345889-28345908 None:intergenic 60.0%
! ACCAGTTCCCCATGACTCGG+CGG - chr1.2:28345856-28345875 None:intergenic 60.0%
! ATGACTCGGCGGAGGTTTGG+TGG - chr1.2:28345845-28345864 None:intergenic 60.0%
! CACCTGGTGGGCATTTGGGT+AGG + chr1.2:28346390-28346409 MS.gene53546:CDS 60.0%
! CGCCGTTGTTTCGGCTATCG+CGG + chr1.2:28346031-28346050 MS.gene53546:CDS 60.0%
! TGGTACCGAAGGCGCGAAAG+CGG + chr1.2:28346298-28346317 MS.gene53546:CDS 60.0%
ATTCCTCGTGTCGCCGGAGG+TGG + chr1.2:28345891-28345910 MS.gene53546:CDS 65.0%
CGTATTCCTCGTGTCGCCGG+AGG + chr1.2:28345888-28345907 MS.gene53546:CDS 65.0%
GACGGTGACGAGGGGTCTTG+CGG - chr1.2:28345667-28345686 None:intergenic 65.0%
TCCGCCGAGTCATGGGGAAC+TGG + chr1.2:28345852-28345871 MS.gene53546:CDS 65.0%
TTCCTCGTGTCGCCGGAGGT+GGG + chr1.2:28345892-28345911 MS.gene53546:CDS 65.0%
! AGTTCCCCATGACTCGGCGG+AGG - chr1.2:28345853-28345872 None:intergenic 65.0%
! CCCATGACTCGGCGGAGGTT+TGG - chr1.2:28345848-28345867 None:intergenic 65.0%
! GTGGCCGGCTTTACGGCTGA+CGG - chr1.2:28345826-28345845 None:intergenic 65.0%
AGCACGGTGGGTCCCACCTC+CGG - chr1.2:28345907-28345926 None:intergenic 70.0%
GGTGACGAGGGGTCTTGCGG+CGG - chr1.2:28345664-28345683 None:intergenic 70.0%
!! CTCGGCGGAGGTTTGGTGGC+CGG - chr1.2:28345841-28345860 None:intergenic 70.0%
GACGAGGGGTCTTGCGGCGG+CGG - chr1.2:28345661-28345680 None:intergenic 75.0%
GGAGGTGGGACCCACCGTGC+TGG + chr1.2:28345906-28345925 MS.gene53546:CDS 75.0%
GTCCCACCTCCGGCGACACG+AGG - chr1.2:28345897-28345916 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 28345651 28348419 28345651 ID=MS.gene53546
chr1.2 mRNA 28345651 28348419 28345651 ID=MS.gene53546.t1;Parent=MS.gene53546
chr1.2 exon 28345651 28346757 28345651 ID=MS.gene53546.t1.exon1;Parent=MS.gene53546.t1
chr1.2 CDS 28345651 28346757 28345651 ID=cds.MS.gene53546.t1;Parent=MS.gene53546.t1
chr1.2 exon 28348306 28348419 28348306 ID=MS.gene53546.t1.exon2;Parent=MS.gene53546.t1
chr1.2 CDS 28348306 28348419 28348306 ID=cds.MS.gene53546.t1;Parent=MS.gene53546.t1
Gene Sequence

>MS.gene53546

ATGGCTTCCGCCGCCGCAAGACCCCTCGTCACCGTCCAAACACTCGACTCCGACATGGCCACCGATTCATCAACCACCCTACCAATCCCCGATGTAATGCGAGCTTCAATCCGTCCCGATATCGTCAACTTCGTCCACTCCAACATTTCCAAAAACTCCCGACAACCATACGCCGTCAGCCGTAAAGCCGGCCACCAAACCTCCGCCGAGTCATGGGGAACTGGTCGTGCCGTCTCCCGTATTCCTCGTGTCGCCGGAGGTGGGACCCACCGTGCTGGTCAAGCTGCTTTCGGTAACATGTGCCGTGGTGGTCGTATGTTTGCTCCGACCAGAATCTGGCGTAAATGGCACCGGAAAATCAATGTGAATCAGAAGCGTTACGCCGTTGTTTCGGCTATCGCGGCTTCGGCGATTCCATCGCTTGTTCTAGCTCGTGGTCATAGGATTGAAACTGTTCCTGAATTTCCTCTTGTTGTTGGTGATTCAGCTGAAGGAGTTGAGAAAACTAAGGAAGCTATTAGTGTTTTGAAGAAGATTGGAGCATTTGCTGATGCTGAGAAAGCGAAAGCGAGTATTGGGATTCGACCGGGGAAAGGTAAGATGCGTAACCGTAGGTATATATCGCGAAAAGGACCGTTGATTGTTTATGGTACCGAAGGCGCGAAAGCGGTGAAAGCATTTAGGAATATACCTGGTGTTGAGATTACTAATGTTGAGAGGTTGAATCTTTTGAAACTTGCACCTGGTGGGCATTTGGGTAGGTTTGTTGTTTGGACTAAATCGGCTTTTGAGAAACTTGATTCGATTTACGGGACTTTTGATAAGGGTAGTGAGAAGAAAAAAGGGTATGTTTTACCTAGGGCTAAGATGGTTAATTCTGATTTGACTAGGATTATTAACAGTGATGAGGTGCAGAGTGTTGTGAGGCCGATTAAGAAGGATGTTAAGCGCGCTACGCTTAAGAAGAATCCTCTTAAGAATCTTAATGTTATGCTTAGACTTAATCCTTATGCTAAGACTGCTAAGAGGATGGCTTTGCTTGCTGAGGCTGAGAGGGTTAAGGCTAAGAAGGAGAAGCTTGATAAGAAGAGGAAGACTGTTTCTAAGGTGATACTTTTACTTGTATCAATTAAATATTTTGTTCAAAATGTTTATAGTTTGTTACTTGTATGCATATTTTGTTTTGATGTTTCATTTTGTGGGCATGTTCATTTCATTATTACGTTAAATGTTGAATTATGTGCTAAGCCTTATTGCACTTTGATTTGAATCGTACTATTATTACGTTAAATGTTGAATTATGTGCTAAGCCTTATTGCACTTTGATTTGAATCGTACTATAATTTGATTTGTTCTCTGCTAGTTTCTATGTACCTTAGAAATTATGTGCTAATTGCACTTTGATTTGAATTGTACATGCATGCACAACCAGTTGGACACTCTATTTGGGCACACATACATAATTGAGAATATGCAACGAGAACATAGTAAAATAAATGTCTATTTTGAAACTAATTAAATGAAATGTATTTGTCTGTGTGCACCTAAATGATGTTCACAAAAGCTTGTGTCCAAGCAAGACTTCCTCTAGTGTTATGTATCACAGTGGAATTTTGATGTATGTTTGTTCTTAATATTAGTATTATGTTAAATGTTGAATTACGTGCTAATTGCATTTTGATCTCAATAGCACTTTATTTGTATTGTGCTTTGAACTGAGTTTTACTTTGCTGGTTTCTATGTACCTATTCTGCTGTGCATAGTGTAATGTTGTTTGGTTTAGATGTAACCTTGTTTTAATTTCTATCATTGACATGTGTTTAGGTCTGATCATGCAATGTAAAATTTATGATGATAGTTAGTGCTGTCAAATAGATGGTGGCTATAGAATAGGCTGCTATTACGGTGTGATTTAGTTCCAAGTATTGTCAACTAGTGGTGCTACTGCTCTGTATCGTAGTGGAATTTGAACTAACTTCTATTTTCTATAATTTACGATTTACATCATACAATCTCTGATGACCCCGGTTTCATAGCAATGCCATTGTGCTTCGTTCTGTTGTGTTTTCTTCAAATGATGCAATTAAGGATCAATCATTGTTTCAAATATGACTTTTTATGTCATATCATATAATCATATTCCATTTACAAAAGGTCCAGAGATTAGCAAGTTAGATAGAAATATGGTATTTCATATAACATTATAACGTTGTTTGATCAATGGAGCCGATCCCCCTTAGTCACATAAGGATTGGTTGTTGGTGTTGTTGTATCATATTTCCATTTACATTTCTGTTTGGTATATAAGCATACACTGTATTCTTATATCATAAAACTAAGTTTATGTCTTAATGACAACTTTTGGTGCATTTCAATTGTGTCCTTAATTGCAACCGATGCTTCACTTTTGATTATCCTCTATTGAATTGCAGTTAATGATCAAATGTGCTTTAGTATTATATAGATTTAATCATGTATGTAGATATAAGTTAGTTGTGTTCGAGAAATGAAAGATTTTTTGTATGATGTTTCTATGTGTTCTCTCTGATGCCTTTGCTCTATTTCCTGCATTGTAATTGTTTTATGTTTTATTATTAGGCATTTGTTTCAATATTGTTCATGGTTTAAAATTGACATTATTTTCCTTTGTTTCAGGAGGAAGCTTCTGCCATTAAGGCAGCTGGAAAAGCTTGGTACAACACTATGGTCTCGGATTCAGATTATACAGAATTTGACAACTTCTCCAAGTGGTTGGGTGTTTCACAGTGA
Protein sequence

>MS.gene53546.t1

MASAAARPLVTVQTLDSDMATDSSTTLPIPDVMRASIRPDIVNFVHSNISKNSRQPYAVSRKAGHQTSAESWGTGRAVSRIPRVAGGGTHRAGQAAFGNMCRGGRMFAPTRIWRKWHRKINVNQKRYAVVSAIAASAIPSLVLARGHRIETVPEFPLVVGDSAEGVEKTKEAISVLKKIGAFADAEKAKASIGIRPGKGKMRNRRYISRKGPLIVYGTEGAKAVKAFRNIPGVEITNVERLNLLKLAPGGHLGRFVVWTKSAFEKLDSIYGTFDKGSEKKKGYVLPRAKMVNSDLTRIINSDEVQSVVRPIKKDVKRATLKKNPLKNLNVMLRLNPYAKTAKRMALLAEAERVKAKKEKLDKKRKTVSKEEASAIKAAGKAWYNTMVSDSDYTEFDNFSKWLGVSQ