Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32556.t1 | RHN80631.1 | 81.8 | 962 | 120 | 2 | 1 | 907 | 33 | 994 | 0.00E+00 | 1375.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32556.t1 | Q9NW13 | 40.7 | 492 | 254 | 8 | 242 | 705 | 112 | 593 | 4.0e-53 | 211.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32556.t1 | G7I6T9 | 81.8 | 962 | 120 | 2 | 1 | 907 | 1 | 962 | 0.0e+00 | 1375.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene32556 | 0.80703 | 6.17E-50 | -1.69E-46 |
| MS.gene049159 | MS.gene32556 | 0.817865 | 2.64E-52 | -1.69E-46 |
| MS.gene049207 | MS.gene32556 | 0.807152 | 5.81E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene32556 | 0.845185 | 4.75E-59 | -1.69E-46 |
| MS.gene049343 | MS.gene32556 | 0.817245 | 3.64E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene32556 | 0.80102 | 1.10E-48 | -1.69E-46 |
| MS.gene049501 | MS.gene32556 | 0.834464 | 2.95E-56 | -1.69E-46 |
| MS.gene049567 | MS.gene32556 | 0.805611 | 1.23E-49 | -1.69E-46 |
| MS.gene049824 | MS.gene32556 | 0.818758 | 1.66E-52 | -1.69E-46 |
| MS.gene049825 | MS.gene32556 | 0.813719 | 2.22E-51 | -1.69E-46 |
| MS.gene049850 | MS.gene32556 | 0.80823 | 3.43E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene32556 | 0.805266 | 1.45E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene32556 | 0.812524 | 4.06E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene32556 | 0.807812 | 4.21E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene32556 | 0.819823 | 9.48E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene32556 | 0.826944 | 2.05E-54 | -1.69E-46 |
| MS.gene050442 | MS.gene32556 | 0.808039 | 3.77E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene32556 | 0.81807 | 2.37E-52 | -1.69E-46 |
| MS.gene050561 | MS.gene32556 | 0.818549 | 1.85E-52 | -1.69E-46 |
| MS.gene050562 | MS.gene32556 | 0.812439 | 4.24E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene32556 | 0.824594 | 7.42E-54 | -1.69E-46 |
| MS.gene050721 | MS.gene32556 | 0.821669 | 3.57E-53 | -1.69E-46 |
| MS.gene050724 | MS.gene32556 | 0.825467 | 4.61E-54 | -1.69E-46 |
| MS.gene050780 | MS.gene32556 | 0.801588 | 8.39E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene32556 | 0.818763 | 1.65E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene32556 | 0.810517 | 1.11E-50 | -1.69E-46 |
| MS.gene051574 | MS.gene32556 | 0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene051700 | MS.gene32556 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene32556 | 0.863143 | 3.07E-64 | -1.69E-46 |
| MS.gene052190 | MS.gene32556 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene32556 | 0.815989 | 6.97E-52 | -1.69E-46 |
| MS.gene052333 | MS.gene32556 | 0.812661 | 3.79E-51 | -1.69E-46 |
| MS.gene052384 | MS.gene32556 | 0.826343 | 2.86E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene32556 | 0.839592 | 1.45E-57 | -1.69E-46 |
| MS.gene052582 | MS.gene32556 | 0.835676 | 1.46E-56 | -1.69E-46 |
| MS.gene052584 | MS.gene32556 | 0.846329 | 2.33E-59 | -1.69E-46 |
| MS.gene052611 | MS.gene32556 | 0.814546 | 1.46E-51 | -1.69E-46 |
| MS.gene052863 | MS.gene32556 | 0.805896 | 1.07E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene32556 | 0.805085 | 1.58E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene32556 | 0.820602 | 6.29E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene32556 | 0.818158 | 2.27E-52 | -1.69E-46 |
| MS.gene053079 | MS.gene32556 | 0.80084 | 1.19E-48 | -1.69E-46 |
| MS.gene053080 | MS.gene32556 | 0.804006 | 2.66E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene32556 | 0.857237 | 1.87E-62 | -1.69E-46 |
| MS.gene053995 | MS.gene32556 | 0.806424 | 8.28E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene32556 | 0.804765 | 1.85E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene32556 | 0.805692 | 1.18E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene32556 | 0.835691 | 1.45E-56 | -1.69E-46 |
| MS.gene054657 | MS.gene32556 | 0.824253 | 8.92E-54 | -1.69E-46 |
| MS.gene054658 | MS.gene32556 | 0.807934 | 3.97E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene32556 | 0.813681 | 2.26E-51 | -1.69E-46 |
| MS.gene055166 | MS.gene32556 | 0.838738 | 2.41E-57 | -1.69E-46 |
| MS.gene055227 | MS.gene32556 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene32556 | 0.823551 | 1.30E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene32556 | 0.807471 | 4.97E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene32556 | 0.826111 | 3.25E-54 | -1.69E-46 |
| MS.gene055589 | MS.gene32556 | 0.82579 | 3.87E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene32556 | 0.833222 | 6.04E-56 | -1.69E-46 |
| MS.gene055818 | MS.gene32556 | 0.833984 | 3.90E-56 | -1.69E-46 |
| MS.gene05604 | MS.gene32556 | 0.832586 | 8.69E-56 | -1.69E-46 |
| MS.gene056050 | MS.gene32556 | 0.825675 | 4.12E-54 | -1.69E-46 |
| MS.gene056366 | MS.gene32556 | 0.810961 | 8.89E-51 | -1.69E-46 |
| MS.gene056403 | MS.gene32556 | 0.810077 | 1.38E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene32556 | 0.850057 | 2.17E-60 | -1.69E-46 |
| MS.gene05659 | MS.gene32556 | 0.809563 | 1.78E-50 | -1.69E-46 |
| MS.gene056605 | MS.gene32556 | 0.801935 | 7.12E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene32556 | 0.811229 | 7.77E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene32556 | 0.843965 | 1.01E-58 | -1.69E-46 |
| MS.gene057060 | MS.gene32556 | 0.807681 | 4.49E-50 | -1.69E-46 |
| MS.gene057314 | MS.gene32556 | 0.856544 | 2.99E-62 | -1.69E-46 |
| MS.gene057915 | MS.gene32556 | 0.844743 | 6.26E-59 | -1.69E-46 |
| MS.gene058010 | MS.gene32556 | 0.807213 | 5.64E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene32556 | 0.850836 | 1.31E-60 | -1.69E-46 |
| MS.gene058548 | MS.gene32556 | 0.813299 | 2.75E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene32556 | 0.831037 | 2.09E-55 | -1.69E-46 |
| MS.gene058829 | MS.gene32556 | 0.845493 | 3.93E-59 | -1.69E-46 |
| MS.gene058979 | MS.gene32556 | 0.838006 | 3.71E-57 | -1.69E-46 |
| MS.gene05954 | MS.gene32556 | 0.824591 | 7.43E-54 | -1.69E-46 |
| MS.gene059570 | MS.gene32556 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene32556 | 0.8157 | 8.08E-52 | -1.69E-46 |
| MS.gene059725 | MS.gene32556 | 0.803044 | 4.21E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene32556 | 0.807091 | 5.99E-50 | -1.69E-46 |
| MS.gene059830 | MS.gene32556 | 0.806037 | 9.99E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene32556 | 0.806691 | 7.28E-50 | -1.69E-46 |
| MS.gene059935 | MS.gene32556 | 0.824845 | 6.47E-54 | -1.69E-46 |
| MS.gene060011 | MS.gene32556 | 0.800467 | 1.42E-48 | -1.69E-46 |
| MS.gene060521 | MS.gene32556 | 0.806356 | 8.56E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene32556 | 0.835337 | 1.78E-56 | -1.69E-46 |
| MS.gene060637 | MS.gene32556 | 0.815808 | 7.65E-52 | -1.69E-46 |
| MS.gene060690 | MS.gene32556 | 0.80137 | 9.30E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene32556 | 0.803934 | 2.75E-49 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32556.t1 | MTR_1g080390 | 89.917 | 962 | 42 | 2 | 1 | 907 | 1 | 962 | 0.0 | 1741 |
| MS.gene32556.t1 | MTR_4g118630 | 40.000 | 75 | 45 | 0 | 246 | 320 | 50 | 124 | 8.89e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32556.t1 | AT2G21440 | 46.626 | 978 | 401 | 22 | 20 | 907 | 57 | 1003 | 0.0 | 725 |
Find 188 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCTTCCTTTGGTTCTTT+TGG | 0.103039 | 5.1:-62154398 | None:intergenic |
| TCTGTTTCCTTGGAAGTTTC+AGG | 0.151548 | 5.1:-62159476 | None:intergenic |
| TGCTTCTGGGATGGGTATTT+TGG | 0.157301 | 5.1:+62159780 | MS.gene32556:CDS |
| CGTTACGAAGTTTCAGTTTC+TGG | 0.161347 | 5.1:-62160975 | None:intergenic |
| CCTTGTTCGCTATTTGAATT+TGG | 0.165477 | 5.1:-62161144 | None:intergenic |
| GGGGTTCTCTTTCTGTCTTT+TGG | 0.176034 | 5.1:-62161208 | None:intergenic |
| ACTGAATCTTTGTTCTTCTT+TGG | 0.190926 | 5.1:-62161462 | None:intergenic |
| GTAACAGTTTCCTTGGATTT+TGG | 0.211651 | 5.1:-62159398 | None:intergenic |
| AAGAAAGAAGACGCGATATT+TGG | 0.226860 | 5.1:+62161384 | MS.gene32556:CDS |
| TCAACTGCCAATACTGCTTC+TGG | 0.228699 | 5.1:+62159766 | MS.gene32556:CDS |
| GGTAAAGGGTAGACCATTAA+AGG | 0.229384 | 5.1:+62159801 | MS.gene32556:CDS |
| GTTGCTAGAACTGTTATATT+TGG | 0.244405 | 5.1:+62154787 | MS.gene32556:CDS |
| AGAGCCTTTAAAACCTTTAA+TGG | 0.253378 | 5.1:-62159814 | None:intergenic |
| TTTCATCTGCGGGGCCTGTA+TGG | 0.269359 | 5.1:+62156412 | MS.gene32556:CDS |
| GATAAAGACAGCGGATTATC+TGG | 0.273260 | 5.1:+62154353 | MS.gene32556:CDS |
| AGGCCAAAGAAAATGAAATA+AGG | 0.273524 | 5.1:+62156382 | MS.gene32556:intron |
| AAACTCAATGGATCGAAGTT+TGG | 0.305477 | 5.1:+62156718 | MS.gene32556:CDS |
| TCCACAATGGGCCGATGTTC+AGG | 0.311484 | 5.1:-62160934 | None:intergenic |
| CACTTCGGAATTTGTACTCT+TGG | 0.314322 | 5.1:-62161229 | None:intergenic |
| ACTTCGGAATTTGTACTCTT+GGG | 0.316199 | 5.1:-62161228 | None:intergenic |
| TTGCCCGCCGAAGACGCTTT+TGG | 0.316252 | 5.1:-62161270 | None:intergenic |
| TCTACGCAACATCGTGGTTT+TGG | 0.321066 | 5.1:+62153019 | MS.gene32556:CDS |
| TAACCAACCTGCCGAATTAC+AGG | 0.332513 | 5.1:-62160456 | None:intergenic |
| CAATCACAACCTTCTTCCTT+TGG | 0.333819 | 5.1:-62154407 | None:intergenic |
| TGTCAGAAACCTTCCTTTCA+AGG | 0.342388 | 5.1:+62156277 | MS.gene32556:CDS |
| CAAATCATCTTCTGTTTCCT+TGG | 0.348953 | 5.1:-62159486 | None:intergenic |
| GTCGTCTCATAGCTGTTGAT+TGG | 0.358840 | 5.1:+62156743 | MS.gene32556:CDS |
| AACTCAATGGATCGAAGTTT+GGG | 0.358983 | 5.1:+62156719 | MS.gene32556:CDS |
| ATTATTTACAGATTCGTCTT+TGG | 0.361831 | 5.1:-62159345 | None:intergenic |
| ATAGTTCCATTGAAAGTAAT+AGG | 0.371133 | 5.1:+62161349 | MS.gene32556:CDS |
| AAGCAGGGGAAAAGGTTTCT+AGG | 0.374787 | 5.1:+62161433 | MS.gene32556:CDS |
| CTATTAAGCATCCCCTTTCA+AGG | 0.383007 | 5.1:+62154878 | MS.gene32556:CDS |
| CACCATGACTCTGGCCATCT+TGG | 0.383423 | 5.1:-62161305 | None:intergenic |
| GCTGAAACCCCTTCGGCAGC+TGG | 0.384100 | 5.1:-62160036 | None:intergenic |
| AGAAAGTCCAAAAGCGTCTT+CGG | 0.389500 | 5.1:+62161263 | MS.gene32556:CDS |
| AGAACCCTTGTGTGAGAACT+TGG | 0.390972 | 5.1:-62161535 | None:intergenic |
| CTCTGAAGCAGGAGCATCAT+TGG | 0.391724 | 5.1:-62156797 | None:intergenic |
| AGCGTAAACTCCTGCATTGT+CGG | 0.396945 | 5.1:-62161061 | None:intergenic |
| CACCGCCAAGCCAGAGAAAT+CGG | 0.405483 | 5.1:+62154844 | MS.gene32556:CDS |
| AAATCGTTGCTTCACTTCTT+CGG | 0.405889 | 5.1:-62159552 | None:intergenic |
| ATCTAATTCAAAGGGAAGAT+TGG | 0.406524 | 5.1:-62159528 | None:intergenic |
| AAGCTTCCATTTCTGAGTCT+TGG | 0.414414 | 5.1:-62156252 | None:intergenic |
| AGACCACCGCAATCTTTACT+TGG | 0.416144 | 5.1:+62159892 | MS.gene32556:CDS |
| TGCACCCGTAACAGTTTCCT+TGG | 0.420231 | 5.1:-62159405 | None:intergenic |
| CCAAGCAAACACCTGTAATT+CGG | 0.424020 | 5.1:+62160445 | MS.gene32556:CDS |
| TTCATCTGCGGGGCCTGTAT+GGG | 0.425013 | 5.1:+62156413 | MS.gene32556:CDS |
| TGAGCTGAAGAACGGTTCAT+TGG | 0.431497 | 5.1:+62154081 | MS.gene32556:CDS |
| GGCCAAAGAAAATGAAATAA+GGG | 0.433746 | 5.1:+62156383 | MS.gene32556:intron |
| TCCTGTGTGCAGTCTTTCGA+AGG | 0.439780 | 5.1:+62156558 | MS.gene32556:intron |
| ACACACAGTGCCGATTTCTC+TGG | 0.447857 | 5.1:-62154854 | None:intergenic |
| TGCCGATTTCTCTGGCTTGG+CGG | 0.449281 | 5.1:-62154846 | None:intergenic |
| TTGGTCACTTGGTGGAGAAC+CGG | 0.449419 | 5.1:-62159605 | None:intergenic |
| CAACTGCCAATACTGCTTCT+GGG | 0.451408 | 5.1:+62159767 | MS.gene32556:CDS |
| AATCTAAACAGCTTAGGAAA+TGG | 0.456031 | 5.1:+62161583 | MS.gene32556:CDS |
| TGAACCGTTCTTCAGCTCAA+TGG | 0.460449 | 5.1:-62154077 | None:intergenic |
| CTAGGACATACTTGGTCACT+TGG | 0.461965 | 5.1:-62159616 | None:intergenic |
| CAGAAGATCATTCCTTGAAA+GGG | 0.462491 | 5.1:-62154890 | None:intergenic |
| GCCAATACTGCTTCTGGGAT+GGG | 0.465705 | 5.1:+62159772 | MS.gene32556:CDS |
| GCGTAAACTCCTGCATTGTC+GGG | 0.469405 | 5.1:-62161060 | None:intergenic |
| TTGTCTTGTGAATTCTGCTA+TGG | 0.470061 | 5.1:+62154810 | MS.gene32556:CDS |
| AAAATCCAAGGAAACTGTTA+CGG | 0.470073 | 5.1:+62159400 | MS.gene32556:CDS |
| CCAAGAACAAGCAGGGGAAA+AGG | 0.470314 | 5.1:+62161425 | MS.gene32556:CDS |
| ACCCATCCCAGAAGCAGTAT+TGG | 0.471074 | 5.1:-62159773 | None:intergenic |
| CCGAATTACAGGTGTTTGCT+TGG | 0.472209 | 5.1:-62160445 | None:intergenic |
| TTTGGGCACGCCAACTTGGT+GGG | 0.473129 | 5.1:+62155546 | MS.gene32556:CDS |
| ACTGTTTGGGCACGCCAACT+TGG | 0.473827 | 5.1:+62155542 | MS.gene32556:CDS |
| CGTTCTTCAGCTCAATGGCT+TGG | 0.475932 | 5.1:-62154072 | None:intergenic |
| TAGGAGGGACTTATTCTTGA+CGG | 0.477564 | 5.1:+62160009 | MS.gene32556:intron |
| CAGGCGATTCGAAAACTCAA+TGG | 0.479592 | 5.1:+62156706 | MS.gene32556:intron |
| TGCAGGCGTCCACGAGGGAC+TGG | 0.480915 | 5.1:+62159700 | MS.gene32556:intron |
| AGAGTTCTTAACAATAATCC+TGG | 0.483615 | 5.1:+62160686 | MS.gene32556:CDS |
| AAATCCAAGGAAACTGTTAC+GGG | 0.485581 | 5.1:+62159401 | MS.gene32556:CDS |
| TACAGATTCGTCTTTGGCAG+AGG | 0.486047 | 5.1:-62159339 | None:intergenic |
| TGCCAATACTGCTTCTGGGA+TGG | 0.486395 | 5.1:+62159771 | MS.gene32556:CDS |
| GTTTGGGCACGCCAACTTGG+TGG | 0.487001 | 5.1:+62155545 | MS.gene32556:CDS |
| TAAGAAAAGCAAAGCAATGA+AGG | 0.487047 | 5.1:+62152651 | MS.gene32556:CDS |
| CAGTGCCGATTTCTCTGGCT+TGG | 0.490360 | 5.1:-62154849 | None:intergenic |
| GATTTCTTTAGGACAACAAA+AGG | 0.491246 | 5.1:+62159139 | MS.gene32556:intron |
| AGCATCAATCAACCTTGATC+CGG | 0.491692 | 5.1:-62154172 | None:intergenic |
| CAAGGTGGTAAAGTGCCAAA+TGG | 0.499530 | 5.1:+62161162 | MS.gene32556:CDS |
| TCGTCTCATAGCTGTTGATT+GGG | 0.499678 | 5.1:+62156744 | MS.gene32556:CDS |
| AAGAACAGCCTTCTTGCAGC+AGG | 0.499780 | 5.1:-62155433 | None:intergenic |
| TTCTCACACAAGGGTTCTCA+AGG | 0.501028 | 5.1:+62161540 | MS.gene32556:CDS |
| ACGGTTCATTGGTTGGTGAC+CGG | 0.501056 | 5.1:+62154092 | MS.gene32556:CDS |
| AGGGAGCCAAGACTCAGAAA+TGG | 0.503890 | 5.1:+62156246 | MS.gene32556:intron |
| GAAAGTAATAGGAAAATCTC+AGG | 0.503966 | 5.1:+62161360 | MS.gene32556:CDS |
| GTAATTTAGCACCATGACTC+TGG | 0.504247 | 5.1:-62161314 | None:intergenic |
| AGAAATATCTGAAACCTTGT+CGG | 0.504739 | 5.1:-62159450 | None:intergenic |
| GGGAAAAGGTTTCTAGGAAA+AGG | 0.509364 | 5.1:+62161439 | MS.gene32556:CDS |
| TGAAAATCGAAGATCAAAAC+CGG | 0.510715 | 5.1:+62154153 | MS.gene32556:CDS |
| ATGCAAAACCAAGAACAAGC+AGG | 0.511718 | 5.1:+62161417 | MS.gene32556:CDS |
| TCTTCGGTATCTAATTCAAA+GGG | 0.513395 | 5.1:-62159536 | None:intergenic |
| CTTCGGAATTTGTACTCTTG+GGG | 0.515980 | 5.1:-62161227 | None:intergenic |
| GCAGAAGATCATTCCTTGAA+AGG | 0.517219 | 5.1:-62154891 | None:intergenic |
| TCAAGAGCAAACTCCACAAT+GGG | 0.517340 | 5.1:-62160946 | None:intergenic |
| TCAACAGATCGAACCTTGAA+AGG | 0.517773 | 5.1:-62156290 | None:intergenic |
| GGAATGAATACCTCCCATAC+AGG | 0.518282 | 5.1:-62156426 | None:intergenic |
| ATTCAAGAAAAGCGACGCCA+CGG | 0.519166 | 5.1:-62160631 | None:intergenic |
| TTCAAGAAAAGCGACGCCAC+GGG | 0.519586 | 5.1:-62160630 | None:intergenic |
| CGAAGATCAAAACCGGATCA+AGG | 0.520422 | 5.1:+62154160 | MS.gene32556:CDS |
| ACTGACAAAGAATCTGATCA+AGG | 0.522199 | 5.1:+62159209 | MS.gene32556:CDS |
| ATCAGTGGCTGAAACCCCTT+CGG | 0.522666 | 5.1:-62160043 | None:intergenic |
| TTCTTCGGTATCTAATTCAA+AGG | 0.523243 | 5.1:-62159537 | None:intergenic |
| GACGGAACTCCAGCTGCCGA+AGG | 0.523716 | 5.1:+62160027 | MS.gene32556:CDS |
| CAATGATGCTCCTGCTTCAG+AGG | 0.523848 | 5.1:+62156798 | MS.gene32556:CDS |
| CAGATCGAACCTTGAAAGGA+AGG | 0.524767 | 5.1:-62156286 | None:intergenic |
| GAAATATTTATAGTTTGGAA+AGG | 0.525585 | 5.1:+62160289 | MS.gene32556:intron |
| TTGTAATGACGCAGCAGATG+AGG | 0.526196 | 5.1:+62154579 | MS.gene32556:CDS |
| GAACAAAGATTCAGTAGGGA+AGG | 0.527716 | 5.1:+62161470 | MS.gene32556:CDS |
| AGTCCAAAAGCGTCTTCGGC+GGG | 0.531268 | 5.1:+62161267 | MS.gene32556:CDS |
| CCAAAAGAACCAAAGGAAGA+AGG | 0.533227 | 5.1:+62154398 | MS.gene32556:CDS |
| AAGACGCGATATTTGGAAAG+AGG | 0.533754 | 5.1:+62161391 | MS.gene32556:CDS |
| AAGAAGAACAAAGATTCAGT+AGG | 0.534680 | 5.1:+62161465 | MS.gene32556:CDS |
| AAATTACCTTCCTCTGAAGC+AGG | 0.535871 | 5.1:-62156808 | None:intergenic |
| TGCATTATCAGCTGCTTCCG+CGG | 0.535875 | 5.1:-62159740 | None:intergenic |
| TGTAATGACGCAGCAGATGA+GGG | 0.537350 | 5.1:+62154580 | MS.gene32556:CDS |
| TTCCCAGGCCTGCTGCAAGA+AGG | 0.537740 | 5.1:+62155425 | MS.gene32556:intron |
| AATGATAAACCCGACAATGC+AGG | 0.540063 | 5.1:+62161051 | MS.gene32556:CDS |
| TTCTTTCGATGATATCACTT+CGG | 0.542100 | 5.1:-62161244 | None:intergenic |
| TTCTAAGCCTGAAACTTCCA+AGG | 0.542108 | 5.1:+62159469 | MS.gene32556:CDS |
| AAGGGTTCTCAAGGAAATGA+TGG | 0.544012 | 5.1:+62161549 | MS.gene32556:CDS |
| CCTGTGTGCAGTCTTTCGAA+GGG | 0.545229 | 5.1:+62156559 | MS.gene32556:intron |
| GACACAATAGAGGGCAATGA+AGG | 0.546836 | 5.1:+62154305 | MS.gene32556:intron |
| GATGCTCCTGCTTCAGAGGA+AGG | 0.546927 | 5.1:+62156802 | MS.gene32556:CDS |
| GGACATACTTGGTCACTTGG+TGG | 0.548184 | 5.1:-62159613 | None:intergenic |
| TGCAAAACCAAGAACAAGCA+GGG | 0.550150 | 5.1:+62161418 | MS.gene32556:CDS |
| AAAGAACAAGCCAAAATCCA+AGG | 0.552898 | 5.1:+62159388 | MS.gene32556:CDS |
| ACGGAACTCCAGCTGCCGAA+GGG | 0.553197 | 5.1:+62160028 | MS.gene32556:CDS |
| TCATTGACCACAGAAACCTT+CGG | 0.555563 | 5.1:+62160910 | MS.gene32556:intron |
| TTGGGCACGCCAACTTGGTG+GGG | 0.556141 | 5.1:+62155547 | MS.gene32556:CDS |
| ACTTTACCTCCCCACCAAGT+TGG | 0.556299 | 5.1:-62155556 | None:intergenic |
| GCAAACACCTGTAATTCGGC+AGG | 0.559184 | 5.1:+62160449 | MS.gene32556:CDS |
| GGCACGCCAACTTGGTGGGG+AGG | 0.559247 | 5.1:+62155550 | MS.gene32556:CDS |
| TTCAGTTCCAAAAGAACCAA+AGG | 0.560178 | 5.1:+62154391 | MS.gene32556:CDS |
| CTGAAGAACGGTTCATTGGT+TGG | 0.563589 | 5.1:+62154085 | MS.gene32556:CDS |
| AGAAGAACAAAGATTCAGTA+GGG | 0.570029 | 5.1:+62161466 | MS.gene32556:CDS |
| GAAAGATGGCAGGAAAGGAA+AGG | 0.571880 | 5.1:+62160586 | MS.gene32556:CDS |
| ATCTGCGGGGCCTGTATGGG+AGG | 0.572499 | 5.1:+62156416 | MS.gene32556:CDS |
| TCCTGAACATCGGCCCATTG+TGG | 0.574904 | 5.1:+62160933 | MS.gene32556:CDS |
| GGGCCGATGTTCAGGACCGA+AGG | 0.576328 | 5.1:-62160926 | None:intergenic |
| CATGGTGCTAAATTACATGA+AGG | 0.582212 | 5.1:+62161321 | MS.gene32556:CDS |
| CCAAATTCAAATAGCGAACA+AGG | 0.584745 | 5.1:+62161144 | MS.gene32556:CDS |
| CTGACAAAGAATCTGATCAA+GGG | 0.585118 | 5.1:+62159210 | MS.gene32556:CDS |
| TAAGAACTCTCAACGCAACA+AGG | 0.587382 | 5.1:-62160673 | None:intergenic |
| CCGCAATCTTTACTTGGCGA+AGG | 0.588434 | 5.1:+62159898 | MS.gene32556:CDS |
| CGGAACTCCAGCTGCCGAAG+GGG | 0.589924 | 5.1:+62160029 | MS.gene32556:CDS |
| AAAGCTACAGTATCAGCAGC+GGG | 0.590881 | 5.1:+62160999 | MS.gene32556:CDS |
| AATGCAGGAGTTTACGCTCA+TGG | 0.594723 | 5.1:+62161066 | MS.gene32556:CDS |
| TGACAAACCAGCAAAAGACT+TGG | 0.595099 | 5.1:+62161119 | MS.gene32556:CDS |
| AACCAAGATGGCCAGAGTCA+TGG | 0.597159 | 5.1:+62161303 | MS.gene32556:CDS |
| AGGAATGATCTTCTGCAGCA+TGG | 0.605435 | 5.1:+62154898 | MS.gene32556:CDS |
| TACCCTGTGTCGGACTTCTG+TGG | 0.606080 | 5.1:-62156447 | None:intergenic |
| TAATGACGCAGCAGATGAGG+GGG | 0.606529 | 5.1:+62154582 | MS.gene32556:CDS |
| ATTCCACAGAAGTCCGACAC+AGG | 0.608649 | 5.1:+62156444 | MS.gene32556:CDS |
| ATCAAGAGCAAACTCCACAA+TGG | 0.611104 | 5.1:-62160947 | None:intergenic |
| AAGTCCAAAAGCGTCTTCGG+CGG | 0.614715 | 5.1:+62161266 | MS.gene32556:CDS |
| TTTCTGTTGTTGCCGTTGTG+AGG | 0.616515 | 5.1:-62161024 | None:intergenic |
| AAGGAATATATACCCTGTGT+CGG | 0.616888 | 5.1:-62156457 | None:intergenic |
| TGACAAAGAATCTGATCAAG+GGG | 0.619765 | 5.1:+62159211 | MS.gene32556:CDS |
| TAGATCCAAGTTCTCACACA+AGG | 0.623451 | 5.1:+62161530 | MS.gene32556:CDS |
| GTAATGACGCAGCAGATGAG+GGG | 0.624059 | 5.1:+62154581 | MS.gene32556:CDS |
| CAAGCCATTGAGCTGAAGAA+CGG | 0.627871 | 5.1:+62154073 | MS.gene32556:CDS |
| CCAAATGGAAAATCTCCTCA+AGG | 0.629918 | 5.1:+62161177 | MS.gene32556:CDS |
| AAGCTGAAAAGTAACCAAGA+TGG | 0.637677 | 5.1:+62161291 | MS.gene32556:CDS |
| AGAAGATCATTCCTTGAAAG+GGG | 0.638195 | 5.1:-62154889 | None:intergenic |
| AAACCTTCGGTCCTGAACAT+CGG | 0.642171 | 5.1:+62160923 | MS.gene32556:CDS |
| CAGCAGCGGGCTCCTCACAA+CGG | 0.643240 | 5.1:+62161012 | MS.gene32556:CDS |
| AGATAGTAGTACTACTACTG+AGG | 0.650531 | 5.1:+62159172 | MS.gene32556:CDS |
| GCAAAACCAAGAACAAGCAG+GGG | 0.651326 | 5.1:+62161419 | MS.gene32556:CDS |
| TAGTGATATTGATAGTGTTG+TGG | 0.658825 | 5.1:+62159238 | MS.gene32556:CDS |
| CTCAATGCAGGCGTCCACGA+GGG | 0.661595 | 5.1:+62159695 | MS.gene32556:intron |
| TGATATTGATAGTGTTGTGG+AGG | 0.663472 | 5.1:+62159241 | MS.gene32556:CDS |
| TTCCACAGAAGTCCGACACA+GGG | 0.667711 | 5.1:+62156445 | MS.gene32556:CDS |
| CAAGAACAGCAGTATTCCCG+TGG | 0.668300 | 5.1:+62160614 | MS.gene32556:CDS |
| TTAAGAAAACCAGTCCCTCG+TGG | 0.669577 | 5.1:-62159709 | None:intergenic |
| TCAGGGTCTACGCAACATCG+TGG | 0.673144 | 5.1:+62153013 | MS.gene32556:intron |
| AACATCTAAAGAGTCCGACA+AGG | 0.679032 | 5.1:+62159436 | MS.gene32556:CDS |
| ACTCAATGCAGGCGTCCACG+AGG | 0.680693 | 5.1:+62159694 | MS.gene32556:intron |
| AAAAGCTACAGTATCAGCAG+CGG | 0.686433 | 5.1:+62160998 | MS.gene32556:CDS |
| GCAATGAAGGAAAATAGCGA+CGG | 0.697380 | 5.1:+62152664 | MS.gene32556:CDS |
| AAGAACTCTCAACGCAACAA+GGG | 0.697627 | 5.1:-62160672 | None:intergenic |
| AGATCCAAGTTCTCACACAA+GGG | 0.701976 | 5.1:+62161531 | MS.gene32556:CDS |
| TCGCCAAGTAAAGATTGCGG+TGG | 0.704028 | 5.1:-62159895 | None:intergenic |
| AGATTCAGTAGGGAAGGACA+TGG | 0.712089 | 5.1:+62161476 | MS.gene32556:CDS |
| AATGGAAAATCTCCTCAAGG+AGG | 0.725686 | 5.1:+62161180 | MS.gene32556:CDS |
| CCTTCGCCAAGTAAAGATTG+CGG | 0.731180 | 5.1:-62159898 | None:intergenic |
| AATGACGCAGCAGATGAGGG+GGG | 0.732596 | 5.1:+62154583 | MS.gene32556:CDS |
| AATTCAAATAGCGAACAAGG+TGG | 0.748003 | 5.1:+62161147 | MS.gene32556:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 62152646 | 62161617 | 62152646 | ID=MS.gene32556 |
| chr5.1 | mRNA | 62152646 | 62161617 | 62152646 | ID=MS.gene32556.t1;Parent=MS.gene32556 |
| chr5.1 | exon | 62152646 | 62152700 | 62152646 | ID=MS.gene32556.t1.exon1;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62152646 | 62152700 | 62152646 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62153017 | 62153053 | 62153017 | ID=MS.gene32556.t1.exon2;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62153017 | 62153053 | 62153017 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62154051 | 62154181 | 62154051 | ID=MS.gene32556.t1.exon3;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62154051 | 62154181 | 62154051 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62154315 | 62154419 | 62154315 | ID=MS.gene32556.t1.exon4;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62154315 | 62154419 | 62154315 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62154515 | 62154623 | 62154515 | ID=MS.gene32556.t1.exon5;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62154515 | 62154623 | 62154515 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62154786 | 62154919 | 62154786 | ID=MS.gene32556.t1.exon6;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62154786 | 62154919 | 62154786 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62155432 | 62155571 | 62155432 | ID=MS.gene32556.t1.exon7;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62155432 | 62155571 | 62155432 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62156248 | 62156298 | 62156248 | ID=MS.gene32556.t1.exon8;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62156248 | 62156298 | 62156248 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62156384 | 62156466 | 62156384 | ID=MS.gene32556.t1.exon9;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62156384 | 62156466 | 62156384 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62156570 | 62156624 | 62156570 | ID=MS.gene32556.t1.exon10;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62156570 | 62156624 | 62156570 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62156709 | 62156823 | 62156709 | ID=MS.gene32556.t1.exon11;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62156709 | 62156823 | 62156709 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62159150 | 62159627 | 62159150 | ID=MS.gene32556.t1.exon12;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62159150 | 62159627 | 62159150 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62159705 | 62159822 | 62159705 | ID=MS.gene32556.t1.exon13;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62159705 | 62159822 | 62159705 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62159875 | 62159919 | 62159875 | ID=MS.gene32556.t1.exon14;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62159875 | 62159919 | 62159875 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62160012 | 62160082 | 62160012 | ID=MS.gene32556.t1.exon15;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62160012 | 62160082 | 62160012 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62160302 | 62160470 | 62160302 | ID=MS.gene32556.t1.exon16;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62160302 | 62160470 | 62160302 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62160575 | 62160707 | 62160575 | ID=MS.gene32556.t1.exon17;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62160575 | 62160707 | 62160575 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| chr5.1 | exon | 62160923 | 62161617 | 62160923 | ID=MS.gene32556.t1.exon18;Parent=MS.gene32556.t1 |
| chr5.1 | CDS | 62160923 | 62161617 | 62160923 | ID=cds.MS.gene32556.t1;Parent=MS.gene32556.t1 |
| Gene Sequence |
| Protein sequence |
