Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64243.t1 | XP_024630994.1 | 96.7 | 61 | 2 | 0 | 1 | 61 | 20 | 80 | 1.10E-22 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64243.t1 | Q9T0H2 | 59.7 | 62 | 24 | 1 | 1 | 61 | 1 | 62 | 1.8e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64243.t1 | A0A1S2XYN4 | 73.8 | 61 | 15 | 1 | 1 | 61 | 1 | 60 | 7.9e-15 | 88.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene64243 | 0.815812 | 7.63E-52 | -1.69E-46 |
| MS.gene049255 | MS.gene64243 | 0.851933 | 6.43E-61 | -1.69E-46 |
| MS.gene049424 | MS.gene64243 | 0.807019 | 6.20E-50 | -1.69E-46 |
| MS.gene049653 | MS.gene64243 | 0.813848 | 2.08E-51 | -1.69E-46 |
| MS.gene049756 | MS.gene64243 | 0.803391 | 3.57E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene64243 | 0.841666 | 4.14E-58 | -1.69E-46 |
| MS.gene050303 | MS.gene64243 | 0.838748 | 2.39E-57 | -1.69E-46 |
| MS.gene050442 | MS.gene64243 | 0.803091 | 4.11E-49 | -1.69E-46 |
| MS.gene050605 | MS.gene64243 | 0.803387 | 3.57E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene64243 | -0.814931 | 1.20E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene64243 | 0.808479 | 3.04E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene64243 | 0.812044 | 5.17E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene64243 | 0.823016 | 1.74E-53 | -1.69E-46 |
| MS.gene050807 | MS.gene64243 | 0.812371 | 4.38E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene64243 | 0.853992 | 1.66E-61 | -1.69E-46 |
| MS.gene050953 | MS.gene64243 | 0.801393 | 9.20E-49 | -1.69E-46 |
| MS.gene050987 | MS.gene64243 | 0.809228 | 2.10E-50 | -1.69E-46 |
| MS.gene051088 | MS.gene64243 | 0.812327 | 4.48E-51 | -1.69E-46 |
| MS.gene051241 | MS.gene64243 | 0.841289 | 5.20E-58 | -1.69E-46 |
| MS.gene051243 | MS.gene64243 | 0.871445 | 6.80E-67 | -1.69E-46 |
| MS.gene051245 | MS.gene64243 | 0.825979 | 3.49E-54 | -1.69E-46 |
| MS.gene051328 | MS.gene64243 | 0.838955 | 2.11E-57 | -1.69E-46 |
| MS.gene051349 | MS.gene64243 | 0.821272 | 4.41E-53 | -1.69E-46 |
| MS.gene05134 | MS.gene64243 | 0.843124 | 1.70E-58 | -1.69E-46 |
| MS.gene051451 | MS.gene64243 | 0.809852 | 1.54E-50 | -1.69E-46 |
| MS.gene051452 | MS.gene64243 | 0.848014 | 8.03E-60 | -1.69E-46 |
| MS.gene051535 | MS.gene64243 | 0.802341 | 5.87E-49 | -1.69E-46 |
| MS.gene051633 | MS.gene64243 | 0.811418 | 7.07E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene64243 | 0.802425 | 5.65E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene64243 | 0.813733 | 2.20E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene64243 | 0.814533 | 1.47E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene64243 | 0.838782 | 2.34E-57 | -1.69E-46 |
| MS.gene052176 | MS.gene64243 | 0.807453 | 5.02E-50 | -1.69E-46 |
| MS.gene052286 | MS.gene64243 | 0.810237 | 1.27E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene64243 | 0.804615 | 1.98E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene64243 | 0.818961 | 1.49E-52 | -1.69E-46 |
| MS.gene052426 | MS.gene64243 | 0.82769 | 1.36E-54 | -1.69E-46 |
| MS.gene052954 | MS.gene64243 | 0.817724 | 2.84E-52 | -1.69E-46 |
| MS.gene053006 | MS.gene64243 | 0.823665 | 1.23E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene64243 | 0.850605 | 1.52E-60 | -1.69E-46 |
| MS.gene053058 | MS.gene64243 | 0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene64243 | 0.817461 | 3.26E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene64243 | 0.809504 | 1.83E-50 | -1.69E-46 |
| MS.gene053931 | MS.gene64243 | 0.807922 | 3.99E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene64243 | 0.855615 | 5.60E-62 | -1.69E-46 |
| MS.gene054658 | MS.gene64243 | 0.835072 | 2.08E-56 | -1.69E-46 |
| MS.gene05510 | MS.gene64243 | 0.82 | 8.64E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene64243 | 0.85525 | 7.16E-62 | -1.69E-46 |
| MS.gene055222 | MS.gene64243 | 0.80352 | 3.35E-49 | -1.69E-46 |
| MS.gene055512 | MS.gene64243 | 0.80746 | 5.00E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene64243 | 0.868734 | 5.24E-66 | -1.69E-46 |
| MS.gene055808 | MS.gene64243 | 0.804977 | 1.67E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene64243 | 0.809588 | 1.76E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene64243 | 0.822344 | 2.49E-53 | -1.69E-46 |
| MS.gene056498 | MS.gene64243 | 0.800288 | 1.55E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene64243 | 0.823605 | 1.27E-53 | -1.69E-46 |
| MS.gene056631 | MS.gene64243 | 0.856977 | 2.23E-62 | -1.69E-46 |
| MS.gene056760 | MS.gene64243 | 0.847127 | 1.41E-59 | -1.69E-46 |
| MS.gene056986 | MS.gene64243 | 0.808835 | 2.55E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene64243 | 0.829889 | 4.00E-55 | -1.69E-46 |
| MS.gene057079 | MS.gene64243 | 0.819874 | 9.24E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene64243 | 0.85373 | 1.97E-61 | -1.69E-46 |
| MS.gene057208 | MS.gene64243 | 0.812127 | 4.96E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene64243 | 0.80444 | 2.16E-49 | -1.69E-46 |
| MS.gene057392 | MS.gene64243 | 0.817235 | 3.66E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene64243 | 0.863579 | 2.25E-64 | -1.69E-46 |
| MS.gene057705 | MS.gene64243 | 0.809324 | 2.00E-50 | -1.69E-46 |
| MS.gene05795 | MS.gene64243 | 0.810664 | 1.03E-50 | -1.69E-46 |
| MS.gene058084 | MS.gene64243 | 0.828797 | 7.36E-55 | -1.69E-46 |
| MS.gene058126 | MS.gene64243 | 0.809844 | 1.55E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene64243 | 0.822347 | 2.49E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene64243 | 0.830094 | 3.56E-55 | -1.69E-46 |
| MS.gene058548 | MS.gene64243 | 0.827718 | 1.34E-54 | -1.69E-46 |
| MS.gene058549 | MS.gene64243 | 0.82925 | 5.72E-55 | -1.69E-46 |
| MS.gene058595 | MS.gene64243 | 0.822043 | 2.92E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene64243 | 0.847682 | 9.91E-60 | -1.69E-46 |
| MS.gene058976 | MS.gene64243 | 0.804633 | 1.97E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene64243 | 0.805784 | 1.13E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene64243 | 0.80169 | 7.99E-49 | -1.69E-46 |
| MS.gene059249 | MS.gene64243 | 0.810161 | 1.32E-50 | -1.69E-46 |
| MS.gene059317 | MS.gene64243 | 0.818235 | 2.18E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene64243 | 0.80441 | 2.19E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene64243 | 0.832985 | 6.91E-56 | -1.69E-46 |
| MS.gene05979 | MS.gene64243 | 0.850551 | 1.58E-60 | -1.69E-46 |
| MS.gene059830 | MS.gene64243 | 0.846744 | 1.79E-59 | -1.69E-46 |
| MS.gene059842 | MS.gene64243 | 0.83887 | 2.22E-57 | -1.69E-46 |
| MS.gene060297 | MS.gene64243 | 0.810782 | 9.72E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene64243 | 0.806522 | 7.90E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene64243 | 0.835646 | 1.49E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene64243 | 0.853338 | 2.55E-61 | -1.69E-46 |
| MS.gene060572 | MS.gene64243 | 0.810341 | 1.21E-50 | -1.69E-46 |
| MS.gene060586 | MS.gene64243 | 0.805089 | 1.58E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene64243 | 0.811985 | 5.32E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene64243 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene64243 | 0.81291 | 3.34E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene64243 | 0.855799 | 4.95E-62 | -1.69E-46 |
| MS.gene060907 | MS.gene64243 | 0.820783 | 5.72E-53 | -1.69E-46 |
| MS.gene060998 | MS.gene64243 | 0.816979 | 4.18E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64243.t1 | MTR_1g045890 | 94.286 | 35 | 2 | 0 | 1 | 35 | 20 | 54 | 6.88e-17 | 67.4 |
| MS.gene64243.t1 | MTR_1g045890 | 75.000 | 48 | 11 | 1 | 1 | 47 | 20 | 67 | 1.01e-16 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCGACTTTGCTATCATTGA+CGG | 0.418660 | 1.3:-27554294 | None:intergenic |
| ATGAGAACAATGGATTTGTT+CGG | 0.449405 | 1.3:+27554188 | MS.gene64243:CDS |
| ATCATTGACGGCAATACCTT+CGG | 0.457365 | 1.3:-27554282 | None:intergenic |
| GGATTTGTTCGGAGAGGAGA+TGG | 0.472087 | 1.3:+27554199 | MS.gene64243:CDS |
| CAGCATCATCGAAATCATCT+TGG | 0.487074 | 1.3:-27554340 | None:intergenic |
| TCAGATAACAACCATGGCTA+TGG | 0.492608 | 1.3:+27554226 | MS.gene64243:CDS |
| ATCATCGAAATCATCTTGGA+AGG | 0.593027 | 1.3:-27554336 | None:intergenic |
| AACAATGGATTTGTTCGGAG+AGG | 0.601463 | 1.3:+27554193 | MS.gene64243:CDS |
| AATTTCGATATCTTATCCGA+AGG | 0.608255 | 1.3:+27554266 | MS.gene64243:CDS |
| TTTGTTCGGAGAGGAGATGG+AGG | 0.616272 | 1.3:+27554202 | MS.gene64243:CDS |
| GTCATCAACATCCATAGCCA+TGG | 0.620045 | 1.3:-27554237 | None:intergenic |
| TAGTCGAAGAATGAGAACAA+TGG | 0.638863 | 1.3:+27554178 | None:intergenic |
| GGAGGATCAGATAACAACCA+TGG | 0.690661 | 1.3:+27554220 | MS.gene64243:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTTCGATATCTTATCCGA+AGG | + | chr1.3:27554266-27554285 | MS.gene64243:CDS | 30.0% | |
| ! | ATGAGAACAATGGATTTGTT+CGG | + | chr1.3:27554188-27554207 | MS.gene64243:CDS | 30.0% |
| ATCATCGAAATCATCTTGGA+AGG | - | chr1.3:27554339-27554358 | None:intergenic | 35.0% | |
| ATCATTGACGGCAATACCTT+CGG | - | chr1.3:27554285-27554304 | None:intergenic | 40.0% | |
| CAGCATCATCGAAATCATCT+TGG | - | chr1.3:27554343-27554362 | None:intergenic | 40.0% | |
| TCAGATAACAACCATGGCTA+TGG | + | chr1.3:27554226-27554245 | MS.gene64243:CDS | 40.0% | |
| ! | AACAATGGATTTGTTCGGAG+AGG | + | chr1.3:27554193-27554212 | MS.gene64243:CDS | 40.0% |
| ! | AGCGACTTTGCTATCATTGA+CGG | - | chr1.3:27554297-27554316 | None:intergenic | 40.0% |
| GGAGGATCAGATAACAACCA+TGG | + | chr1.3:27554220-27554239 | MS.gene64243:CDS | 45.0% | |
| GTCATCAACATCCATAGCCA+TGG | - | chr1.3:27554240-27554259 | None:intergenic | 45.0% | |
| TTTGTTCGGAGAGGAGATGG+AGG | + | chr1.3:27554202-27554221 | MS.gene64243:CDS | 50.0% | |
| ! | GGATTTGTTCGGAGAGGAGA+TGG | + | chr1.3:27554199-27554218 | MS.gene64243:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 27554188 | 27554373 | 27554188 | ID=MS.gene64243 |
| chr1.3 | mRNA | 27554188 | 27554373 | 27554188 | ID=MS.gene64243.t1;Parent=MS.gene64243 |
| chr1.3 | exon | 27554188 | 27554373 | 27554188 | ID=MS.gene64243.t1.exon1;Parent=MS.gene64243.t1 |
| chr1.3 | CDS | 27554188 | 27554373 | 27554188 | ID=cds.MS.gene64243.t1;Parent=MS.gene64243.t1 |
| Gene Sequence |
| Protein sequence |
