AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77034


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77034.t1 MTR_4g029130 88.947 190 21 0 1 190 1 190 2.67e-108 308
MS.gene77034.t1 MTR_7g072540 55.080 187 75 4 1 181 1 184 2.85e-48 157
MS.gene77034.t1 MTR_5g083970 53.608 97 38 2 11 101 15 110 1.07e-23 93.6
MS.gene77034.t1 MTR_3g072360 47.872 94 42 1 11 97 19 112 4.89e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTGCAGTTCCATTTGTT+TGG 0.199775 4.3:-70507221 MS.gene77034:CDS
AAAATCTTCCAAGTATCTTT+TGG 0.274979 4.3:-70507082 MS.gene77034:CDS
GGTCGTCCCCGAGATAATTT+TGG 0.290058 4.3:+70506948 None:intergenic
TGTTGCAGTTCCATTTGTTT+GGG 0.310743 4.3:-70507220 MS.gene77034:CDS
GTCTTTAGAGAATGTGTGTT+TGG 0.374019 4.3:+70507179 None:intergenic
CCAACTTCTACTTTGTGCTT+TGG 0.382263 4.3:-70506847 MS.gene77034:CDS
AATGTGTGTTTGGGAGTTCC+AGG 0.387249 4.3:+70507189 None:intergenic
TCTGAAAGATGGGTGACAAT+TGG 0.414182 4.3:+70507248 None:intergenic
CACGCCTCTTGTAGTTACTT+TGG 0.419541 4.3:+70506791 None:intergenic
AAGCAATGCCAAAAGATACT+TGG 0.426442 4.3:+70507074 None:intergenic
GATAAGAAGGTGGAGGTGTA+AGG 0.431589 4.3:+70507145 None:intergenic
GATTCTTCCAAAATTATCTC+GGG 0.433513 4.3:-70506955 MS.gene77034:CDS
CGTTCGATCAGCTCGAATAG+TGG 0.451934 4.3:-70506817 MS.gene77034:CDS
ATAAGAAGGTGGAGGTGTAA+GGG 0.477682 4.3:+70507146 None:intergenic
TCTTTAGAGAATGTGTGTTT+GGG 0.478199 4.3:+70507180 None:intergenic
AGATTCTTCCAAAATTATCT+CGG 0.480227 4.3:-70506956 MS.gene77034:CDS
GCAACAGAAACTCTGAAAGA+TGG 0.487509 4.3:+70507237 None:intergenic
GGTTGAGATTCCCAAACAAA+TGG 0.489006 4.3:+70507210 None:intergenic
AGAAGAAAATTCTTAAGCTA+TGG 0.489299 4.3:-70506916 MS.gene77034:CDS
TAAGAAGGTGGAGGTGTAAG+GGG 0.511212 4.3:+70507147 None:intergenic
CCAAAGCACAAAGTAGAAGT+TGG 0.519370 4.3:+70506847 None:intergenic
CAACAGAAACTCTGAAAGAT+GGG 0.528072 4.3:+70507238 None:intergenic
TTTCAGCAGACTACTTTCAA+AGG 0.534157 4.3:-70507277 MS.gene77034:CDS
TTCAGGGGTGAAAATGAAGA+TGG 0.539909 4.3:-70506880 MS.gene77034:CDS
TTTGTTTGGGAATCTCAACC+TGG 0.562667 4.3:-70507207 MS.gene77034:CDS
GCATTGTTGAAATGATAAGA+AGG 0.591984 4.3:+70507132 None:intergenic
GAAGGTGGAGGTGTAAGGGG+AGG 0.592725 4.3:+70507150 None:intergenic
AATTATCTCGGGGACGACCA+AGG 0.607137 4.3:-70506944 MS.gene77034:CDS
TTGATGGCAATGAAAACGAA+GGG 0.630243 4.3:+70506980 None:intergenic
GCTTCCAAAGTAACTACAAG+AGG 0.633252 4.3:-70506795 MS.gene77034:CDS
ATTGATGGCAATGAAAACGA+AGG 0.635141 4.3:+70506979 None:intergenic
TTGTTGAAATGATAAGAAGG+TGG 0.647192 4.3:+70507135 None:intergenic
TTGAAATGATAAGAAGGTGG+AGG 0.647831 4.3:+70507138 None:intergenic
ATTCTTCCAAAATTATCTCG+GGG 0.650660 4.3:-70506954 MS.gene77034:CDS
CGATGACAAAGAAGACGATG+AGG 0.693458 4.3:+70507008 None:intergenic
AGACGATGAGGACAAAGACG+AGG 0.726177 4.3:+70507020 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAAAATTCTTAAGCTA+TGG - chr4.3:70507200-70507219 MS.gene77034:CDS 25.0%
! AGATTCTTCCAAAATTATCT+CGG - chr4.3:70507160-70507179 MS.gene77034:CDS 25.0%
!! AAAATCTTCCAAGTATCTTT+TGG - chr4.3:70507034-70507053 MS.gene77034:CDS 25.0%
!!! AAGATTTTGAGTGCTTTTTT+TGG + chr4.3:70507022-70507041 None:intergenic 25.0%
!!! ATTTTGGAAGAATCTATTGA+TGG + chr4.3:70507155-70507174 None:intergenic 25.0%
!!! CTTTTTTTGGTTCTTTTTGT+TGG + chr4.3:70507009-70507028 None:intergenic 25.0%
ATTCTTCCAAAATTATCTCG+GGG - chr4.3:70507162-70507181 MS.gene77034:CDS 30.0%
GATTCTTCCAAAATTATCTC+GGG - chr4.3:70507161-70507180 MS.gene77034:CDS 30.0%
GCATTGTTGAAATGATAAGA+AGG + chr4.3:70506987-70507006 None:intergenic 30.0%
TGCTTCAAAAACTAAACTTC+AGG - chr4.3:70507060-70507079 MS.gene77034:CDS 30.0%
TTGTTGAAATGATAAGAAGG+TGG + chr4.3:70506984-70507003 None:intergenic 30.0%
! GAATTTTCTTCTACTAACCT+TGG + chr4.3:70507192-70507211 None:intergenic 30.0%
! TCTTTAGAGAATGTGTGTTT+GGG + chr4.3:70506939-70506958 None:intergenic 30.0%
!!! ATGGATCTTCTTTTGATTTC+AGG - chr4.3:70507219-70507238 MS.gene77034:CDS 30.0%
!!! TGGATCTTCTTTTGATTTCA+GGG - chr4.3:70507220-70507239 MS.gene77034:CDS 30.0%
AAGCAATGCCAAAAGATACT+TGG + chr4.3:70507045-70507064 None:intergenic 35.0%
TGTTGCAGTTCCATTTGTTT+GGG - chr4.3:70506896-70506915 MS.gene77034:CDS 35.0%
TTGAAATGATAAGAAGGTGG+AGG + chr4.3:70506981-70507000 None:intergenic 35.0%
TTTCAGCAGACTACTTTCAA+AGG - chr4.3:70506839-70506858 MS.gene77034:CDS 35.0%
! ATTGATGGCAATGAAAACGA+AGG + chr4.3:70507140-70507159 None:intergenic 35.0%
! CAACAGAAACTCTGAAAGAT+GGG + chr4.3:70506881-70506900 None:intergenic 35.0%
! GTCTTTAGAGAATGTGTGTT+TGG + chr4.3:70506940-70506959 None:intergenic 35.0%
! TTGATGGCAATGAAAACGAA+GGG + chr4.3:70507139-70507158 None:intergenic 35.0%
!!! GGATCTTCTTTTGATTTCAG+GGG - chr4.3:70507221-70507240 MS.gene77034:CDS 35.0%
CCAAAGCACAAAGTAGAAGT+TGG + chr4.3:70507272-70507291 None:intergenic 40.0%
CCAACTTCTACTTTGTGCTT+TGG - chr4.3:70507269-70507288 MS.gene77034:CDS 40.0%
CTGTTGCAGTTCCATTTGTT+TGG - chr4.3:70506895-70506914 MS.gene77034:CDS 40.0%
GCTTCCAAAGTAACTACAAG+AGG - chr4.3:70507321-70507340 MS.gene77034:CDS 40.0%
GGTTGAGATTCCCAAACAAA+TGG + chr4.3:70506909-70506928 None:intergenic 40.0%
TCTGAAAGATGGGTGACAAT+TGG + chr4.3:70506871-70506890 None:intergenic 40.0%
TTCAGGGGTGAAAATGAAGA+TGG - chr4.3:70507236-70507255 MS.gene77034:CDS 40.0%
TTTGTTTGGGAATCTCAACC+TGG - chr4.3:70506909-70506928 MS.gene77034:CDS 40.0%
! ATAAGAAGGTGGAGGTGTAA+GGG + chr4.3:70506973-70506992 None:intergenic 40.0%
! GCAACAGAAACTCTGAAAGA+TGG + chr4.3:70506882-70506901 None:intergenic 40.0%
AATGTGTGTTTGGGAGTTCC+AGG + chr4.3:70506930-70506949 None:intergenic 45.0%
CACGCCTCTTGTAGTTACTT+TGG + chr4.3:70507328-70507347 None:intergenic 45.0%
CGATGACAAAGAAGACGATG+AGG + chr4.3:70507111-70507130 None:intergenic 45.0%
! GATAAGAAGGTGGAGGTGTA+AGG + chr4.3:70506974-70506993 None:intergenic 45.0%
! TAAGAAGGTGGAGGTGTAAG+GGG + chr4.3:70506972-70506991 None:intergenic 45.0%
AATTATCTCGGGGACGACCA+AGG - chr4.3:70507172-70507191 MS.gene77034:CDS 50.0%
AGACGATGAGGACAAAGACG+AGG + chr4.3:70507099-70507118 None:intergenic 50.0%
CGTTCGATCAGCTCGAATAG+TGG - chr4.3:70507299-70507318 MS.gene77034:CDS 50.0%
! GGTCGTCCCCGAGATAATTT+TGG + chr4.3:70507171-70507190 None:intergenic 50.0%
! GAAGGTGGAGGTGTAAGGGG+AGG + chr4.3:70506969-70506988 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 70506783 70507355 70506783 ID=MS.gene77034
chr4.3 mRNA 70506783 70507355 70506783 ID=MS.gene77034.t1;Parent=MS.gene77034
chr4.3 exon 70506783 70507355 70506783 ID=MS.gene77034.t1.exon1;Parent=MS.gene77034.t1
chr4.3 CDS 70506783 70507355 70506783 ID=cds.MS.gene77034.t1;Parent=MS.gene77034.t1
Gene Sequence

>MS.gene77034

ATGTTCAATTCAACATGTGAGAAGAGTGTTCTTCAAATCAAACAAGATGACAAGTTTTTCAGCAGACTACTTTCAAAGGAAAATTCCAATTGTCACCCATCTTTCAGAGTTTCTGTTGCAGTTCCATTTGTTTGGGAATCTCAACCTGGAACTCCCAAACACACATTCTCTAAAGACATTATTCCTCCCCTTACACCTCCACCTTCTTATCATTTCAACAATGCCAACAAAAAGAACCAAAAAAAGCACTCAAAATCTTCCAAGTATCTTTTGGCATTGCTTCAAAAACTAAACTTCAGGAAGAACAATCAGTCCTCGTCTTTGTCCTCATCGTCTTCTTTGTCATCGTCATACCCTTCGTTTTCATTGCCATCAATAGATTCTTCCAAAATTATCTCGGGGACGACCAAGGTTAGTAGAAGAAAATTCTTAAGCTATGGATCTTCTTTTGATTTCAGGGGTGAAAATGAAGATGGTAATGATTCACCAACTTCTACTTTGTGCTTTGGAATTCATCGTTCGATCAGCTCGAATAGTGGCTTCCAAAGTAACTACAAGAGGCGTGTAAAGTAA

Protein sequence

>MS.gene77034.t1

MFNSTCEKSVLQIKQDDKFFSRLLSKENSNCHPSFRVSVAVPFVWESQPGTPKHTFSKDIIPPLTPPPSYHFNNANKKNQKKHSKSSKYLLALLQKLNFRKNNQSSSLSSSSSLSSSYPSFSLPSIDSSKIISGTTKVSRRKFLSYGSSFDFRGENEDGNDSPTSTLCFGIHRSISSNSGFQSNYKRRVK