AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88945


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88945.t1 MTR_1g026125 90.365 301 16 4 10 304 5 298 0.0 534
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88945.t1 AT1G76080 63.898 313 93 6 1 304 1 302 2.18e-136 388

Find 48 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGAGATATACCCTTTCT+TGG 0.236936 1.3:+157878 MS.gene88945:CDS
TGTGGTAGGTATGTTGGTTC+AGG 0.274596 1.3:+158383 MS.gene88945:CDS
CGTTGAGTTTCTTCTTGTTA+TGG 0.286019 1.3:+157926 MS.gene88945:CDS
GAGGGATATGGAAGTTGTTC+AGG 0.305188 1.3:+158325 MS.gene88945:CDS
TGAGGGTTTGCATATGAAAT+TGG 0.324749 1.3:-157574 None:intergenic
AGCTGCTGTGCCTGTTGCTT+TGG 0.336628 1.3:-157724 None:intergenic
GTTGAGTTTCTTCTTGTTAT+GGG 0.337593 1.3:+157927 MS.gene88945:CDS
GAATGAAGGGTAAGGAAGTT+AGG 0.357510 1.3:-157621 None:intergenic
AAGCTTATTGTTCTTGATGT+TGG 0.379209 1.3:+158176 MS.gene88945:CDS
CGTCGTTACATGTTCGGCTT+AGG 0.394333 1.3:-157905 None:intergenic
TACTACGGTGATAACCATTC+AGG 0.418710 1.3:+158089 MS.gene88945:CDS
ACATTGTGGGCCATGTGTTA+AGG 0.424051 1.3:+158205 MS.gene88945:CDS
ACATCACCAACAAGCATGTC+TGG 0.428494 1.3:-158062 None:intergenic
GTGGTAGGTATGTTGGTTCA+GGG 0.432018 1.3:+158384 MS.gene88945:CDS
AACTCATTGGTGAAATTCTC+AGG 0.435220 1.3:+158414 MS.gene88945:CDS
ATTAGAGATGGTAAGATTTG+TGG 0.439626 1.3:+158365 MS.gene88945:CDS
GAAATTGAAGGAGGGATTGA+GGG 0.440827 1.3:-157591 None:intergenic
CGGCTTAGGTCAACCAAGAA+AGG 0.444183 1.3:-157891 None:intergenic
AAGATTTGTGGTAGGTATGT+TGG 0.445880 1.3:+158377 MS.gene88945:CDS
TTAGGATGAGTTGAAATTGA+AGG 0.445917 1.3:-157603 None:intergenic
TGAAATTGAAGGAGGGATTG+AGG 0.455754 1.3:-157592 None:intergenic
GTTGTTATCACCAAAGCAAC+AGG 0.459253 1.3:+157714 MS.gene88945:CDS
ATTCATGAAGAAGAAGCTAT+TGG 0.470180 1.3:+158038 MS.gene88945:CDS
AAGAAGAACGAAGAGAATGA+AGG 0.480454 1.3:-157635 None:intergenic
AGGTATGTTGGTTCAGGGAA+AGG 0.482216 1.3:+158389 MS.gene88945:CDS
ACTCAACGTCGTTACATGTT+CGG 0.489256 1.3:-157911 None:intergenic
ATGTTGGTCTTAAACATTGT+GGG 0.490241 1.3:+158192 MS.gene88945:CDS
AGAAGAACGAAGAGAATGAA+GGG 0.507243 1.3:-157634 None:intergenic
AACGAAGAGAATGAAGGGTA+AGG 0.514490 1.3:-157629 None:intergenic
AGAACTCTGCAAAAGAGAAA+AGG 0.515029 1.3:+157971 MS.gene88945:CDS
GGATGAGTTGAAATTGAAGG+AGG 0.516103 1.3:-157600 None:intergenic
AATAAGTAACACGAACTCCT+TGG 0.520146 1.3:-158439 None:intergenic
TCAGGGAAAGGTGAACTCAT+TGG 0.523543 1.3:+158401 MS.gene88945:CDS
CTTGACAGCTTAATCACTGT+CGG 0.540890 1.3:-158233 None:intergenic
ATTGGTCCAGACATGCTTGT+TGG 0.551371 1.3:+158056 MS.gene88945:CDS
GATGAGTTGAAATTGAAGGA+GGG 0.552894 1.3:-157599 None:intergenic
AGATGTTGAGAAACTTATTG+AGG 0.553117 1.3:+158136 MS.gene88945:CDS
ATTCATTCTACTTCTCGGGT+AGG 0.557229 1.3:+157678 MS.gene88945:CDS
GGCTTAGGTCAACCAAGAAA+GGG 0.570139 1.3:-157890 None:intergenic
GATGTTGGTCTTAAACATTG+TGG 0.582468 1.3:+158191 MS.gene88945:CDS
GTTGGTGATGTGTTATACTA+CGG 0.591943 1.3:+158074 MS.gene88945:CDS
GTGAGTGATTGTAGTAGCCA+TGG 0.593491 1.3:-157547 None:intergenic
GAAGTTGCACTACACCTGAA+TGG 0.604581 1.3:-158103 None:intergenic
TGAGTGATTGTAGTAGCCAT+GGG 0.617890 1.3:-157546 None:intergenic
GAGATGGTAAGATTTGTGGT+AGG 0.631571 1.3:+158369 MS.gene88945:CDS
GTCGGATAAACCTTAACACA+TGG 0.636213 1.3:-158215 None:intergenic
GGTGAAATTCTCAGGTACCA+AGG 0.658840 1.3:+158422 MS.gene88945:CDS
CTCTTGTAGAAAGTGAAGTG+TGG 0.692063 1.3:-158005 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAATTCATTCTACTTCTC+GGG + chr1.3:157674-157693 MS.gene88945:CDS 25.0%
! CAAAAATTCATTCTACTTCT+CGG + chr1.3:157673-157692 MS.gene88945:CDS 25.0%
! TCTCTAATGAACAAAAAAGT+AGG - chr1.3:158353-158372 None:intergenic 25.0%
!!! ACTTTTTTGTTCATTAGAGA+TGG + chr1.3:158353-158372 MS.gene88945:CDS 25.0%
!!! ATGATACTGTTATTTTTGCA+AGG + chr1.3:158264-158283 MS.gene88945:CDS 25.0%
AAGCTTATTGTTCTTGATGT+TGG + chr1.3:158176-158195 MS.gene88945:CDS 30.0%
AGATGTTGAGAAACTTATTG+AGG + chr1.3:158136-158155 MS.gene88945:CDS 30.0%
ATTAGAGATGGTAAGATTTG+TGG + chr1.3:158365-158384 MS.gene88945:CDS 30.0%
ATTCATGAAGAAGAAGCTAT+TGG + chr1.3:158038-158057 MS.gene88945:CDS 30.0%
! ATAGTTGTATGCAGTTTTTG+AGG + chr1.3:158306-158325 MS.gene88945:CDS 30.0%
! CGAGAAGTAGAATGAATTTT+TGG - chr1.3:157675-157694 None:intergenic 30.0%
! GTTGAGTTTCTTCTTGTTAT+GGG + chr1.3:157927-157946 MS.gene88945:CDS 30.0%
! TAGTTGTATGCAGTTTTTGA+GGG + chr1.3:158307-158326 MS.gene88945:CDS 30.0%
! TTAGGATGAGTTGAAATTGA+AGG - chr1.3:157606-157625 None:intergenic 30.0%
!! ATGTTGGTCTTAAACATTGT+GGG + chr1.3:158192-158211 MS.gene88945:CDS 30.0%
!!! GTTATTTTTGCAAGGATGAA+TGG + chr1.3:158272-158291 MS.gene88945:CDS 30.0%
AACTCATTGGTGAAATTCTC+AGG + chr1.3:158414-158433 MS.gene88945:CDS 35.0%
AAGAAGAACGAAGAGAATGA+AGG - chr1.3:157638-157657 None:intergenic 35.0%
AAGATTTGTGGTAGGTATGT+TGG + chr1.3:158377-158396 MS.gene88945:CDS 35.0%
AATAAGTAACACGAACTCCT+TGG - chr1.3:158442-158461 None:intergenic 35.0%
AGAACTCTGCAAAAGAGAAA+AGG + chr1.3:157971-157990 MS.gene88945:CDS 35.0%
AGAAGAACGAAGAGAATGAA+GGG - chr1.3:157637-157656 None:intergenic 35.0%
CATTGAGATATACCCTTTCT+TGG + chr1.3:157878-157897 MS.gene88945:CDS 35.0%
CGTTGAGTTTCTTCTTGTTA+TGG + chr1.3:157926-157945 MS.gene88945:CDS 35.0%
TGAGGGTTTGCATATGAAAT+TGG - chr1.3:157577-157596 None:intergenic 35.0%
! GATGAGTTGAAATTGAAGGA+GGG - chr1.3:157602-157621 None:intergenic 35.0%
!! GATGTTGGTCTTAAACATTG+TGG + chr1.3:158191-158210 MS.gene88945:CDS 35.0%
!! GTTGGTGATGTGTTATACTA+CGG + chr1.3:158074-158093 MS.gene88945:CDS 35.0%
!! TATGCAGTTTTTGAGGGATA+TGG + chr1.3:158313-158332 MS.gene88945:CDS 35.0%
AACGAAGAGAATGAAGGGTA+AGG - chr1.3:157632-157651 None:intergenic 40.0%
ACTCAACGTCGTTACATGTT+CGG - chr1.3:157914-157933 None:intergenic 40.0%
ATTCATTCTACTTCTCGGGT+AGG + chr1.3:157678-157697 MS.gene88945:CDS 40.0%
CTCTTGTAGAAAGTGAAGTG+TGG - chr1.3:158008-158027 None:intergenic 40.0%
CTTGACAGCTTAATCACTGT+CGG - chr1.3:158236-158255 None:intergenic 40.0%
GAAATTGAAGGAGGGATTGA+GGG - chr1.3:157594-157613 None:intergenic 40.0%
GAATGAAGGGTAAGGAAGTT+AGG - chr1.3:157624-157643 None:intergenic 40.0%
GAGATGGTAAGATTTGTGGT+AGG + chr1.3:158369-158388 MS.gene88945:CDS 40.0%
GTCGGATAAACCTTAACACA+TGG - chr1.3:158218-158237 None:intergenic 40.0%
GTTGTTATCACCAAAGCAAC+AGG + chr1.3:157714-157733 MS.gene88945:CDS 40.0%
TACTACGGTGATAACCATTC+AGG + chr1.3:158089-158108 MS.gene88945:CDS 40.0%
TGAAATTGAAGGAGGGATTG+AGG - chr1.3:157595-157614 None:intergenic 40.0%
! GGATGAGTTGAAATTGAAGG+AGG - chr1.3:157603-157622 None:intergenic 40.0%
ACATCACCAACAAGCATGTC+TGG - chr1.3:158065-158084 None:intergenic 45.0%
ACATTGTGGGCCATGTGTTA+AGG + chr1.3:158205-158224 MS.gene88945:CDS 45.0%
ATTGGTCCAGACATGCTTGT+TGG + chr1.3:158056-158075 MS.gene88945:CDS 45.0%
GAAGTTGCACTACACCTGAA+TGG - chr1.3:158106-158125 None:intergenic 45.0%
GAGGGATATGGAAGTTGTTC+AGG + chr1.3:158325-158344 MS.gene88945:CDS 45.0%
GGCTTAGGTCAACCAAGAAA+GGG - chr1.3:157893-157912 None:intergenic 45.0%
GGTGAAATTCTCAGGTACCA+AGG + chr1.3:158422-158441 MS.gene88945:CDS 45.0%
TCAGGGAAAGGTGAACTCAT+TGG + chr1.3:158401-158420 MS.gene88945:CDS 45.0%
!! AGGTATGTTGGTTCAGGGAA+AGG + chr1.3:158389-158408 MS.gene88945:CDS 45.0%
!! GTGGTAGGTATGTTGGTTCA+GGG + chr1.3:158384-158403 MS.gene88945:CDS 45.0%
!! TGTGGTAGGTATGTTGGTTC+AGG + chr1.3:158383-158402 MS.gene88945:CDS 45.0%
CGGCTTAGGTCAACCAAGAA+AGG - chr1.3:157894-157913 None:intergenic 50.0%
CGTCGTTACATGTTCGGCTT+AGG - chr1.3:157908-157927 None:intergenic 50.0%
! AGCTGCTGTGCCTGTTGCTT+TGG - chr1.3:157727-157746 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 157549 158463 157549 ID=MS.gene88945
chr1.3 mRNA 157549 158463 157549 ID=MS.gene88945.t1;Parent=MS.gene88945
chr1.3 exon 157549 158463 157549 ID=MS.gene88945.t1.exon1;Parent=MS.gene88945.t1
chr1.3 CDS 157549 158463 157549 ID=cds.MS.gene88945.t1;Parent=MS.gene88945.t1
Gene Sequence

>MS.gene88945

ATGGCTACTACAATCACTCACTGCACCAATTTCATATGCAAACCCTCAATCCCTCCTTCAATTTCAACTCATCCTAACTTCCTTACCCTTCATTCTCTTCGTTCTTCTTCTTCTTCTTCTTTACCAAAAATTCATTCTACTTCTCGGGTAGGACTGCAGAGACGTGTTGTTATCACCAAAGCAACAGGCACAGCAGCTTCAGATGCTAAGAAGAAAAAAGATGAAAGAGTTCAAAGAGTTCACAGCATAGAAGAGTTCGATGCAGCACTTCAATCTGCAAAAGACAAACTCGTTGTTGTTGAGTACGCTACAAGCGACGACTCAGAAAGCATTGAGATATACCCTTTCTTGGTTGACCTAAGCCGAACATGTAACGACGTTGAGTTTCTTCTTGTTATGGGAGATGAATCAGAGAAAACAAAAGAACTCTGCAAAAGAGAAAAGGTTGATAAAGTACCACACTTCACTTTCTACAAGAGCAAAGAGAAGATTCATGAAGAAGAAGCTATTGGTCCAGACATGCTTGTTGGTGATGTGTTATACTACGGTGATAACCATTCAGGTGTAGTGCAACTTCACAACAGAGAAGATGTTGAGAAACTTATTGAGGATCATAAGATTGATCATAAGCTTATTGTTCTTGATGTTGGTCTTAAACATTGTGGGCCATGTGTTAAGGTTTATCCGACAGTGATTAAGCTGTCAAGACAAATGAATGATACTGTTATTTTTGCAAGGATGAATGGTGATGAGAATGATAGTTGTATGCAGTTTTTGAGGGATATGGAAGTTGTTCAGGTTCCTACTTTTTTGTTCATTAGAGATGGTAAGATTTGTGGTAGGTATGTTGGTTCAGGGAAAGGTGAACTCATTGGTGAAATTCTCAGGTACCAAGGAGTTCGTGTTACTTATTAA

Protein sequence

>MS.gene88945.t1

MATTITHCTNFICKPSIPPSISTHPNFLTLHSLRSSSSSSLPKIHSTSRVGLQRRVVITKATGTAASDAKKKKDERVQRVHSIEEFDAALQSAKDKLVVVEYATSDDSESIEIYPFLVDLSRTCNDVEFLLVMGDESEKTKELCKREKVDKVPHFTFYKSKEKIHEEEAIGPDMLVGDVLYYGDNHSGVVQLHNREDVEKLIEDHKIDHKLIVLDVGLKHCGPCVKVYPTVIKLSRQMNDTVIFARMNGDENDSCMQFLRDMEVVQVPTFLFIRDGKICGRYVGSGKGELIGEILRYQGVRVTY